molecular grade cacl2  (Millipore)


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    Structured Review

    Millipore molecular grade cacl2
    Molecular Grade Cacl2, supplied by Millipore, used in various techniques. Bioz Stars score: 78/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/molecular grade cacl2/product/Millipore
    Average 78 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    molecular grade cacl2 - by Bioz Stars, 2020-02
    78/100 stars

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    High Performance Liquid Chromatography:

    Article Title: Top-Down Proteomic Identification of Furin-Cleaved α-Subunit of Shiga Toxin 2 from Escherichia coli O157:H7 Using MALDI-TOF-TOF-MS/MS
    Article Snippet: .. In Vitro Furin-Induced Cleavage of α -Stx2 One μ L of bacterial cells was harvested with a sterile 1 μ L transfer loop and transferred to a sterile 2 mL microcentrifuge tube containing 300 μ L of a sterile solution of 3 mM molecular grade CaCl2 (Sigma-Aldrich, St. Louis, MO) in HPLC grade water (Burdick and Jackson) and approximately 100 mg of 0.1 mm zirconia-silica beads (BioSpec Products, Bartlesville, OK). .. The tubes were bead-beat for 60 seconds on a reciprocating shaker (Mini-Beadbeater; Biospec Products, Bartlesville, OK) after which they were centrifuged at 14,000 rpm for 2 minutes.

    Polymerase Chain Reaction:

    Article Title: Top-Down Proteomic Identification of Furin-Cleaved α-Subunit of Shiga Toxin 2 from Escherichia coli O157:H7 Using MALDI-TOF-TOF-MS/MS
    Article Snippet: In Vitro Furin-Induced Cleavage of α -Stx2 One μ L of bacterial cells was harvested with a sterile 1 μ L transfer loop and transferred to a sterile 2 mL microcentrifuge tube containing 300 μ L of a sterile solution of 3 mM molecular grade CaCl2 (Sigma-Aldrich, St. Louis, MO) in HPLC grade water (Burdick and Jackson) and approximately 100 mg of 0.1 mm zirconia-silica beads (BioSpec Products, Bartlesville, OK). .. One μ L of furin enzyme (2000 units/mL, New England BioLabs, Ipswich, MA) was added to 30 μ L of bacterial cell supernatant in a sterile 500 μ L thin-walled PCR tube and incubated at 37°C for 10, 30, or 60 minutes.

    Incubation:

    Article Title: Top-Down Proteomic Identification of Furin-Cleaved α-Subunit of Shiga Toxin 2 from Escherichia coli O157:H7 Using MALDI-TOF-TOF-MS/MS
    Article Snippet: In Vitro Furin-Induced Cleavage of α -Stx2 One μ L of bacterial cells was harvested with a sterile 1 μ L transfer loop and transferred to a sterile 2 mL microcentrifuge tube containing 300 μ L of a sterile solution of 3 mM molecular grade CaCl2 (Sigma-Aldrich, St. Louis, MO) in HPLC grade water (Burdick and Jackson) and approximately 100 mg of 0.1 mm zirconia-silica beads (BioSpec Products, Bartlesville, OK). .. One μ L of furin enzyme (2000 units/mL, New England BioLabs, Ipswich, MA) was added to 30 μ L of bacterial cell supernatant in a sterile 500 μ L thin-walled PCR tube and incubated at 37°C for 10, 30, or 60 minutes.

    In Vitro:

    Article Title: Top-Down Proteomic Identification of Furin-Cleaved α-Subunit of Shiga Toxin 2 from Escherichia coli O157:H7 Using MALDI-TOF-TOF-MS/MS
    Article Snippet: .. In Vitro Furin-Induced Cleavage of α -Stx2 One μ L of bacterial cells was harvested with a sterile 1 μ L transfer loop and transferred to a sterile 2 mL microcentrifuge tube containing 300 μ L of a sterile solution of 3 mM molecular grade CaCl2 (Sigma-Aldrich, St. Louis, MO) in HPLC grade water (Burdick and Jackson) and approximately 100 mg of 0.1 mm zirconia-silica beads (BioSpec Products, Bartlesville, OK). .. The tubes were bead-beat for 60 seconds on a reciprocating shaker (Mini-Beadbeater; Biospec Products, Bartlesville, OK) after which they were centrifuged at 14,000 rpm for 2 minutes.

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    Millipore tppe digestion buffer
    <t>TPPE</t> cleaves the reduced pro-BTH6 in the thionin domain. LC-ESI-MS analysis of the digestion of <t>carboxymethylated</t> myc-proBTH6-strep with recombinant TPPE. The acidic domain is cleaved several times with additional sites compared with the oxidized proprotein.
    Tppe Digestion Buffer, supplied by Millipore, used in various techniques. Bioz Stars score: 79/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tppe digestion buffer/product/Millipore
    Average 79 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tppe digestion buffer - by Bioz Stars, 2020-02
    79/100 stars
      Buy from Supplier

    79
    Millipore cytokine homogenate buffer
    Leptin and <t>cytokine</t> levels (IL-6 and TNF- α ) after 4 and 6 weeks on the diets. Consistent with the weight gain and epididymal fat pad weights, leptin levels were significantly higher after 4 and 6 weeks in the HAGE-HF group compared to the other groups. Also consistent with the liver histology and presence of inflammation after 6 weeks on the diet, IL-6 and TNF- α levels were significantly higher in the HAGE-HF group after 6 weeks on the diet.
    Cytokine Homogenate Buffer, supplied by Millipore, used in various techniques. Bioz Stars score: 79/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cytokine homogenate buffer/product/Millipore
    Average 79 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cytokine homogenate buffer - by Bioz Stars, 2020-02
    79/100 stars
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    TPPE cleaves the reduced pro-BTH6 in the thionin domain. LC-ESI-MS analysis of the digestion of carboxymethylated myc-proBTH6-strep with recombinant TPPE. The acidic domain is cleaved several times with additional sites compared with the oxidized proprotein.

    Journal: The Journal of Biological Chemistry

    Article Title: Isolation and Characterization of a Thionin Proprotein-processing Enzyme from Barley *

    doi: 10.1074/jbc.M115.647859

    Figure Lengend Snippet: TPPE cleaves the reduced pro-BTH6 in the thionin domain. LC-ESI-MS analysis of the digestion of carboxymethylated myc-proBTH6-strep with recombinant TPPE. The acidic domain is cleaved several times with additional sites compared with the oxidized proprotein.

    Article Snippet: The reduced and cysteine-carboxymethylated protein samples were dialyzed against TPPE digestion buffer (25 m m MES, 100 m m NaCl, 10 m m CaCl2 , pH 6.5) using Amicon Ultra 10K ultrafiltration centrifugal filters (Millipore) according to the recommendations of the manufacturer.

    Techniques: Mass Spectrometry, Recombinant

    Leptin and cytokine levels (IL-6 and TNF- α ) after 4 and 6 weeks on the diets. Consistent with the weight gain and epididymal fat pad weights, leptin levels were significantly higher after 4 and 6 weeks in the HAGE-HF group compared to the other groups. Also consistent with the liver histology and presence of inflammation after 6 weeks on the diet, IL-6 and TNF- α levels were significantly higher in the HAGE-HF group after 6 weeks on the diet.

    Journal: BioMed Research International

    Article Title: Advanced Glycation End Products Induce Obesity and Hepatosteatosis in CD-1 Wild-Type Mice

    doi: 10.1155/2016/7867852

    Figure Lengend Snippet: Leptin and cytokine levels (IL-6 and TNF- α ) after 4 and 6 weeks on the diets. Consistent with the weight gain and epididymal fat pad weights, leptin levels were significantly higher after 4 and 6 weeks in the HAGE-HF group compared to the other groups. Also consistent with the liver histology and presence of inflammation after 6 weeks on the diet, IL-6 and TNF- α levels were significantly higher in the HAGE-HF group after 6 weeks on the diet.

    Article Snippet: The remaining liver was washed with cold HBSS and suspended in cytokine homogenate buffer (150 mM NaCl, 15 mM Tris, 1 mM CaCl2 ·2H2 O, and 1 mM MgCl2 ·6H2 O, adjusted to pH 7.4) plus 100x protease inhibitor cocktail 1 (Calbiochem, La Jolla, California) to a total volume of 10 mL and homogenized on ice using a Polytron® Homogenizer (Kinematica Inc., Bohemia, NY).

    Techniques: