model lsm510 laser scanning system  (Carl Zeiss)

 
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    Name:
    Scanning Stage 130x85 MAT CAN D
    Description:
    Scanning Stage 130x85 MAT CAN D for Axio Observer 3 5 7 materials stepper motor drive with 2 mm spindle pitch and integrated controller direct connection via CAN bus flat surface for huge specimen travel range 130 mm x 85 mm 86 mm x 50 mm with HD objectives adjustable limit switches max speed 20 mm s resolution 0 1 µm reproducibility 1 µm absolute Accuracy 5 µm tiltable holder for Electronic Coaxial Drive incl CAN cable for mounting frames K 160 x 110 mm and Z Piezo compatible with Axio Observer A1m D1m Z1m and Axiovert 100 135 200
    Catalog Number:
    432034-9001-000
    Price:
    None
    Buy from Supplier


    Structured Review

    Carl Zeiss model lsm510 laser scanning system
    Detection of GFP-L2 fusion protein bound to HeLa cells by fluorescence. (A) Phase-contrast microscopy of a HeLa cell cultured in a spinner flask. HeLa cells cultured in a spinner flask were incubated with GFP-L2(108–126) at 4°C for 1 h (B) and with GFP-L2(108–126) at 4°C for 1 h and at 37°C for 4 h (C). (B and C) The section near the center of a round cell cultured in suspension is presented. HeLa cells cultured on a glass slide were incubated with plain GFP at 4°C for 1 h (D), with GFP-L2(108–126) at 4°C for 1 h (E), and with GFP-L2(108–126) at 4°C for 1 h and at 37°C for 4 h (F). (E) The section near the surfaces of flat cells showed fluorescent dots with parts of nuclei. (F) The section near the centers of cells showed cytoplasmic fluorescence. Fluorescence was examined with a Carl Zeiss <t>LSM510</t> laser scanning confocal system. (D, E, and F) Nuclei were stained with propidium iodide.
    Scanning Stage 130x85 MAT CAN D for Axio Observer 3 5 7 materials stepper motor drive with 2 mm spindle pitch and integrated controller direct connection via CAN bus flat surface for huge specimen travel range 130 mm x 85 mm 86 mm x 50 mm with HD objectives adjustable limit switches max speed 20 mm s resolution 0 1 µm reproducibility 1 µm absolute Accuracy 5 µm tiltable holder for Electronic Coaxial Drive incl CAN cable for mounting frames K 160 x 110 mm and Z Piezo compatible with Axio Observer A1m D1m Z1m and Axiovert 100 135 200
    https://www.bioz.com/result/model lsm510 laser scanning system/product/Carl Zeiss
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    model lsm510 laser scanning system - by Bioz Stars, 2021-06
    97/100 stars

    Images

    1) Product Images from "Human Papillomavirus Type 16 Minor Capsid Protein L2 N-Terminal Region Containing a Common Neutralization Epitope Binds to the Cell Surface and Enters the Cytoplasm"

    Article Title: Human Papillomavirus Type 16 Minor Capsid Protein L2 N-Terminal Region Containing a Common Neutralization Epitope Binds to the Cell Surface and Enters the Cytoplasm

    Journal: Journal of Virology

    doi: 10.1128/JVI.75.5.2331-2336.2001

    Detection of GFP-L2 fusion protein bound to HeLa cells by fluorescence. (A) Phase-contrast microscopy of a HeLa cell cultured in a spinner flask. HeLa cells cultured in a spinner flask were incubated with GFP-L2(108–126) at 4°C for 1 h (B) and with GFP-L2(108–126) at 4°C for 1 h and at 37°C for 4 h (C). (B and C) The section near the center of a round cell cultured in suspension is presented. HeLa cells cultured on a glass slide were incubated with plain GFP at 4°C for 1 h (D), with GFP-L2(108–126) at 4°C for 1 h (E), and with GFP-L2(108–126) at 4°C for 1 h and at 37°C for 4 h (F). (E) The section near the surfaces of flat cells showed fluorescent dots with parts of nuclei. (F) The section near the centers of cells showed cytoplasmic fluorescence. Fluorescence was examined with a Carl Zeiss LSM510 laser scanning confocal system. (D, E, and F) Nuclei were stained with propidium iodide.
    Figure Legend Snippet: Detection of GFP-L2 fusion protein bound to HeLa cells by fluorescence. (A) Phase-contrast microscopy of a HeLa cell cultured in a spinner flask. HeLa cells cultured in a spinner flask were incubated with GFP-L2(108–126) at 4°C for 1 h (B) and with GFP-L2(108–126) at 4°C for 1 h and at 37°C for 4 h (C). (B and C) The section near the center of a round cell cultured in suspension is presented. HeLa cells cultured on a glass slide were incubated with plain GFP at 4°C for 1 h (D), with GFP-L2(108–126) at 4°C for 1 h (E), and with GFP-L2(108–126) at 4°C for 1 h and at 37°C for 4 h (F). (E) The section near the surfaces of flat cells showed fluorescent dots with parts of nuclei. (F) The section near the centers of cells showed cytoplasmic fluorescence. Fluorescence was examined with a Carl Zeiss LSM510 laser scanning confocal system. (D, E, and F) Nuclei were stained with propidium iodide.

    Techniques Used: Fluorescence, Microscopy, Cell Culture, Incubation, Staining

    Related Articles

    Plasmid Preparation:

    Article Title: Technique of retinal gene therapy: delivery of viral vector into the subretinal space
    Article Snippet: The sensitivity of OCT also meant foveal detachment could be achieved with shallow subretinal fluid only (rather than gross foveal elevation visible under the microscope), which minimised foveal stretch. .. Furthermore, an intraoperative OCT volume scan taken at the end of vector injection would enable the calculation of final bleb volume, giving an indication of the dose given. ..

    Injection:

    Article Title: Technique of retinal gene therapy: delivery of viral vector into the subretinal space
    Article Snippet: The sensitivity of OCT also meant foveal detachment could be achieved with shallow subretinal fluid only (rather than gross foveal elevation visible under the microscope), which minimised foveal stretch. .. Furthermore, an intraoperative OCT volume scan taken at the end of vector injection would enable the calculation of final bleb volume, giving an indication of the dose given. ..

    other:

    Article Title: Evaluation and comparison of the new swept source OCT-based IOLMaster 700 with the IOLMaster 500
    Article Snippet: On the IOLMaster 700, the operator can see the whole scan image and visually check the eye geometry and axis of the measurements; also the foveal scan checks the correct fixation by the patient.

    Confocal Microscopy:

    Article Title: Alpha lipoic acid attenuates ER stress and improves glucose uptake through DNAJB3 cochaperone
    Article Snippet: .. Images were acquired using a 25 ×/0.8 numerical aperture (NA) objective (LD LCI Plan‐Apochromat; Carl Zeiss Inc., Oberkochen, Germany) using confocal microscopy on a laser scanning microscope (LSM 780; Carl Zeiss Inc., Oberkochen, Germany), mounted on a laser scanning microscope (LSM) (Zeiss LSM 780; Carl Zeiss Inc.,). .. Images were analyzed using ZEN imaging software (Carl Zeiss Inc.).

    Laser-Scanning Microscopy:

    Article Title: Alpha lipoic acid attenuates ER stress and improves glucose uptake through DNAJB3 cochaperone
    Article Snippet: .. Images were acquired using a 25 ×/0.8 numerical aperture (NA) objective (LD LCI Plan‐Apochromat; Carl Zeiss Inc., Oberkochen, Germany) using confocal microscopy on a laser scanning microscope (LSM 780; Carl Zeiss Inc., Oberkochen, Germany), mounted on a laser scanning microscope (LSM) (Zeiss LSM 780; Carl Zeiss Inc.,). .. Images were analyzed using ZEN imaging software (Carl Zeiss Inc.).

    Article Title: A New Domain in the Toll/IL-1R Domain-Containing Adaptor Inducing IFN-β Factor (TRIF) Protein Amino Terminus Is Important for TRAF3 Association, Protein Stabilization, and Interferon Signaling
    Article Snippet: Alternatively, HeLa cells were co-transfected with expression constructs encoding mCherry-wt TRIF and eGFP-(Δ160-181) TRIF fusion proteins. .. Cells were kept in a humidified, C02 -fed stage-top chamber, and live cell imaging was acquired using the Zeiss LSM510 META laser-scanning microscope with images collected using the same confocal optical slice parameters. .. Using an expression cDNA library constructed from an adult T cell leukemia (ATL) patient-derived cell line CaGT, we isolated a novel cDNA encoding a variant TRIF protein.

    Article Title: GCN5 Regulates FGF Signaling and Activates Selective MYC Target Genes during Early Embryoid Body Differentiation
    Article Snippet: The slides then were washed with PBT and mounted with coverslips using ProLong Gold Antifade mounting medium (Invitrogen, P36930). .. The slides were imaged on a Zeiss LSM 880 laser-scanning microscope. ..

    Live Cell Imaging:

    Article Title: A New Domain in the Toll/IL-1R Domain-Containing Adaptor Inducing IFN-β Factor (TRIF) Protein Amino Terminus Is Important for TRAF3 Association, Protein Stabilization, and Interferon Signaling
    Article Snippet: Alternatively, HeLa cells were co-transfected with expression constructs encoding mCherry-wt TRIF and eGFP-(Δ160-181) TRIF fusion proteins. .. Cells were kept in a humidified, C02 -fed stage-top chamber, and live cell imaging was acquired using the Zeiss LSM510 META laser-scanning microscope with images collected using the same confocal optical slice parameters. .. Using an expression cDNA library constructed from an adult T cell leukemia (ATL) patient-derived cell line CaGT, we isolated a novel cDNA encoding a variant TRIF protein.

    Imaging:

    Article Title: Quantitative secondary electron imaging for work function extraction at atomic level and layer identification of graphene
    Article Snippet: .. In the HIM imaging process, SE images were obtained from a Carl Zeiss Orion Plus scanning helium ion microscope. ..

    Microscopy:

    Article Title: Quantitative secondary electron imaging for work function extraction at atomic level and layer identification of graphene
    Article Snippet: .. In the HIM imaging process, SE images were obtained from a Carl Zeiss Orion Plus scanning helium ion microscope. ..

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  • 99
    Carl Zeiss confocal laser scanning microscope
    Effect of 1/4MIC of 6a and N , O acetal naphthoquinone derivatives 7a , 7b , and 7c on biofilm development. ( A ) Biofilm formed on surfaces and stained with crystal violet. ( B ) <t>Confocal</t> <t>laser</t> <t>scanning</t> microscopy (CLSM). Effects of 1/4MIC of the derivatives 7b (32 µg/mL) and 7c (16 µg/mL) on biofilm formed by the MRSA strain BMB9393, compared with the untreated biofilm (C neg ). Van vancomycin (1/4 MIC; 0.5 µg/mL). NS not significant; ** p value
    Confocal Laser Scanning Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/confocal laser scanning microscope/product/Carl Zeiss
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    confocal laser scanning microscope - by Bioz Stars, 2021-06
    99/100 stars
      Buy from Supplier

    97
    Carl Zeiss model lsm510 laser scanning system
    Detection of GFP-L2 fusion protein bound to HeLa cells by fluorescence. (A) Phase-contrast microscopy of a HeLa cell cultured in a spinner flask. HeLa cells cultured in a spinner flask were incubated with GFP-L2(108–126) at 4°C for 1 h (B) and with GFP-L2(108–126) at 4°C for 1 h and at 37°C for 4 h (C). (B and C) The section near the center of a round cell cultured in suspension is presented. HeLa cells cultured on a glass slide were incubated with plain GFP at 4°C for 1 h (D), with GFP-L2(108–126) at 4°C for 1 h (E), and with GFP-L2(108–126) at 4°C for 1 h and at 37°C for 4 h (F). (E) The section near the surfaces of flat cells showed fluorescent dots with parts of nuclei. (F) The section near the centers of cells showed cytoplasmic fluorescence. Fluorescence was examined with a Carl Zeiss <t>LSM510</t> laser scanning confocal system. (D, E, and F) Nuclei were stained with propidium iodide.
    Model Lsm510 Laser Scanning System, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/model lsm510 laser scanning system/product/Carl Zeiss
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    model lsm510 laser scanning system - by Bioz Stars, 2021-06
    97/100 stars
      Buy from Supplier

    Image Search Results


    Effect of 1/4MIC of 6a and N , O acetal naphthoquinone derivatives 7a , 7b , and 7c on biofilm development. ( A ) Biofilm formed on surfaces and stained with crystal violet. ( B ) Confocal laser scanning microscopy (CLSM). Effects of 1/4MIC of the derivatives 7b (32 µg/mL) and 7c (16 µg/mL) on biofilm formed by the MRSA strain BMB9393, compared with the untreated biofilm (C neg ). Van vancomycin (1/4 MIC; 0.5 µg/mL). NS not significant; ** p value

    Journal: Scientific Reports

    Article Title: Antibiofilm effects of N,O-acetals derived from 2-amino-1,4-naphthoquinone are associated with downregulation of important global virulence regulators in methicillin-resistant Staphylococcus aureus

    doi: 10.1038/s41598-020-76372-z

    Figure Lengend Snippet: Effect of 1/4MIC of 6a and N , O acetal naphthoquinone derivatives 7a , 7b , and 7c on biofilm development. ( A ) Biofilm formed on surfaces and stained with crystal violet. ( B ) Confocal laser scanning microscopy (CLSM). Effects of 1/4MIC of the derivatives 7b (32 µg/mL) and 7c (16 µg/mL) on biofilm formed by the MRSA strain BMB9393, compared with the untreated biofilm (C neg ). Van vancomycin (1/4 MIC; 0.5 µg/mL). NS not significant; ** p value

    Article Snippet: Before visualization, SYTO 9 solution was removed and the material visualized using a confocal laser scanning microscope (Model LSM510 Carl Zeiss Meditec; Jena, Germany).

    Techniques: Staining, Confocal Laser Scanning Microscopy

    Detection of GFP-L2 fusion protein bound to HeLa cells by fluorescence. (A) Phase-contrast microscopy of a HeLa cell cultured in a spinner flask. HeLa cells cultured in a spinner flask were incubated with GFP-L2(108–126) at 4°C for 1 h (B) and with GFP-L2(108–126) at 4°C for 1 h and at 37°C for 4 h (C). (B and C) The section near the center of a round cell cultured in suspension is presented. HeLa cells cultured on a glass slide were incubated with plain GFP at 4°C for 1 h (D), with GFP-L2(108–126) at 4°C for 1 h (E), and with GFP-L2(108–126) at 4°C for 1 h and at 37°C for 4 h (F). (E) The section near the surfaces of flat cells showed fluorescent dots with parts of nuclei. (F) The section near the centers of cells showed cytoplasmic fluorescence. Fluorescence was examined with a Carl Zeiss LSM510 laser scanning confocal system. (D, E, and F) Nuclei were stained with propidium iodide.

    Journal: Journal of Virology

    Article Title: Human Papillomavirus Type 16 Minor Capsid Protein L2 N-Terminal Region Containing a Common Neutralization Epitope Binds to the Cell Surface and Enters the Cytoplasm

    doi: 10.1128/JVI.75.5.2331-2336.2001

    Figure Lengend Snippet: Detection of GFP-L2 fusion protein bound to HeLa cells by fluorescence. (A) Phase-contrast microscopy of a HeLa cell cultured in a spinner flask. HeLa cells cultured in a spinner flask were incubated with GFP-L2(108–126) at 4°C for 1 h (B) and with GFP-L2(108–126) at 4°C for 1 h and at 37°C for 4 h (C). (B and C) The section near the center of a round cell cultured in suspension is presented. HeLa cells cultured on a glass slide were incubated with plain GFP at 4°C for 1 h (D), with GFP-L2(108–126) at 4°C for 1 h (E), and with GFP-L2(108–126) at 4°C for 1 h and at 37°C for 4 h (F). (E) The section near the surfaces of flat cells showed fluorescent dots with parts of nuclei. (F) The section near the centers of cells showed cytoplasmic fluorescence. Fluorescence was examined with a Carl Zeiss LSM510 laser scanning confocal system. (D, E, and F) Nuclei were stained with propidium iodide.

    Article Snippet: Fluorescence was examined with a model LSM510 laser scanning system (Carl Zeiss Co. Ltd., Oberkochen, Germany).

    Techniques: Fluorescence, Microscopy, Cell Culture, Incubation, Staining