mncs  (Lonza)


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    Name:
    Human Bone Marrow Mononuclear Cells MNCs Cryopreserved Cells
    Description:
    Cryopreserved ampule of Human Bone Marrow Mononuclear Cells containing 25 million cells
    Catalog Number:
    2m-125c
    Price:
    None
    Category:
    Primary and Stem Cells
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    Structured Review

    Lonza mncs
    Effects of RSV on TNF-α-induced adhesion of <t>EPCs</t> and <t>MNCs.</t> TNF-α enhanced EPC adhesion, but adhesion was inhibited by RSV in a concentration dependent fashion. (a) Micrographs depicting MNC adhesion to TNF-α-stimulated EPCs pretreated with various RSV concentrations (100×). Scale bar, 50 μm. C, control; T, TNF-α; R, RSV. (b) EPCs were pretreated with various RSV concentrations (0, 1, 10, 25, and 50 μM) for 18 hours and then stimulated with TNF-α (10 ng/mL) for 6 hours. Adhesion to MNCs was assessed. Data represent means ± SDs, n = 5. # P
    Cryopreserved ampule of Human Bone Marrow Mononuclear Cells containing 25 million cells
    https://www.bioz.com/result/mncs/product/Lonza
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mncs - by Bioz Stars, 2021-03
    95/100 stars

    Images

    1) Product Images from "Resveratrol prevents TNF- α-induced VCAM-1 and ICAM-1 upregulation in endothelial progenitor cells via reduction of NF- κB activation"

    Article Title: Resveratrol prevents TNF- α-induced VCAM-1 and ICAM-1 upregulation in endothelial progenitor cells via reduction of NF- κB activation

    Journal: The Journal of International Medical Research

    doi: 10.1177/0300060520945131

    Effects of RSV on TNF-α-induced adhesion of EPCs and MNCs. TNF-α enhanced EPC adhesion, but adhesion was inhibited by RSV in a concentration dependent fashion. (a) Micrographs depicting MNC adhesion to TNF-α-stimulated EPCs pretreated with various RSV concentrations (100×). Scale bar, 50 μm. C, control; T, TNF-α; R, RSV. (b) EPCs were pretreated with various RSV concentrations (0, 1, 10, 25, and 50 μM) for 18 hours and then stimulated with TNF-α (10 ng/mL) for 6 hours. Adhesion to MNCs was assessed. Data represent means ± SDs, n = 5. # P
    Figure Legend Snippet: Effects of RSV on TNF-α-induced adhesion of EPCs and MNCs. TNF-α enhanced EPC adhesion, but adhesion was inhibited by RSV in a concentration dependent fashion. (a) Micrographs depicting MNC adhesion to TNF-α-stimulated EPCs pretreated with various RSV concentrations (100×). Scale bar, 50 μm. C, control; T, TNF-α; R, RSV. (b) EPCs were pretreated with various RSV concentrations (0, 1, 10, 25, and 50 μM) for 18 hours and then stimulated with TNF-α (10 ng/mL) for 6 hours. Adhesion to MNCs was assessed. Data represent means ± SDs, n = 5. # P

    Techniques Used: Concentration Assay

    Related Articles

    Flow Cytometry:

    Article Title: Androgen action augments ischemia-induced, bone marrow progenitor cell-mediated vasculogenesis
    Article Snippet: Mononuclear cell isolation Mononuclear cells (MNCs) from the bone marrow, spleen and blood were isolated using Lympholytes®-M Cell Separation Media according to manufacturer's protocol (Cedarlane, Burlington, Ontario). .. MNCs were suspended in EGM-2 medium (Lonza, Basel, Switzerland) for flow cytometry analysis. .. Progenitor cell populations were identified by co-expression of the hematopoietic stem cell marker Sca1 and the SDF-1 receptor CXCR4, as previously described , .

    Article Title: NG2+/Nestin+ mesenchymal stem cells dictate DTC dormancy in the bone marrow through TGFβ2
    Article Snippet: Bone marrow (BM) cells were flushed from femurs and tibias, red-blood-cell lysis buffer (Lonza) was used for 2 minutes followed by quantification of the GFP+ cells and normalization to the total number of BM cells. .. Flow cytometry and cell sorting Bone marrow cells were flushed, incubated in red-blood cell lysis buffer (Lonza) for 2 minutes and remaining cells were permeabilized with 0.05% Triton (when using intracellular antibodies) and stained using antibodies and conditions in . .. All experiments were performed using BD FACSAria II equipped with FACS Diva software (BD Biosciences).

    Cytometry:

    Article Title: Androgen action augments ischemia-induced, bone marrow progenitor cell-mediated vasculogenesis
    Article Snippet: Mononuclear cell isolation Mononuclear cells (MNCs) from the bone marrow, spleen and blood were isolated using Lympholytes®-M Cell Separation Media according to manufacturer's protocol (Cedarlane, Burlington, Ontario). .. MNCs were suspended in EGM-2 medium (Lonza, Basel, Switzerland) for flow cytometry analysis. .. Progenitor cell populations were identified by co-expression of the hematopoietic stem cell marker Sca1 and the SDF-1 receptor CXCR4, as previously described , .

    Cell Culture:

    Article Title: Angiogenic Mechanisms of Human CD34+ Stem Cell Exosomes in the Repair of Ischemic Hindlimb
    Article Snippet: The cells were purified from mobilized peripheral-blood mononuclear cells (AllCells LLC, Emeryville, CA, USA) with an Isolex 300i device (Baxter Healthcare); cell purity (85–95%) was determined via flow cytometry. .. Both CD34+ cells and MNCs (250,000 cells/mL) were cultured in X-VIVO 10 serum-free cell-culture medium (Lonza Inc. Allendale, NJ) and ultra-pure exosomes were isolated from the conditioned media by ultracentrifugation and separated from the soluble protein fraction on a sucrose gradient . .. The exosomes from both cell types were characterized using dynamic light scattering, flow cytometery analysis, electron microscopy and immunoblotting as described before , .

    Article Title: A peptide derived from the core β-sheet region of TIRAP decoys TLR4 and reduces inflammatory and autoimmune symptoms in murine models
    Article Snippet: HEK-Blue™ hTLR4 cells (InvivoGen, San Diego, CA, USA) were cultured in high-glucose DMEM containing 1% of the penicillin/streptomycin solution, 10% of FBS, and 0·2% of normocin. .. Human peripheral blood mononuclear cells (hPBMCs) and human mononuclear cells (hMNCs) were purchased from Lonza Inc. (Allendale, NJ, USA) and cultured in RPMI 1640 containing 2·05 mM l -glutamine, 1% of the penicillin/streptomycin solution, and 10% of FBS. .. THP-1 cells (kindly gifted by Dr. Chang-Hee Suh, Ajou University, Medical Centre, Suwon, Korea) were cultured in the RPMI 1640 medium supplemented with 1% of the penicillin/streptomycin solution and 10% of FBS and differentiated into macrophages using 80 nM phorbol 12-myristate 13-acetate (PMA; Sigma-Aldrich Co., St. Louis, MO, USA) for 24 h. All the cells were incubated in a humidified atmosphere containing 5% of CO2 at 37 °C (Thermo Fisher Scientific, Inc.) and the media were refreshed after 18 h of incubation.

    Article Title: Mesodermal Progenitor Cells (MPCs) Differentiate into Mesenchymal Stromal Cells (MSCs) by Activation of Wnt5/Calmodulin Signalling Pathway
    Article Snippet: MSC cultures were obtained from bone marrow mononuclear cells (BMMNCs) grown under standard conditions, using DMEM (Invitrogen, Carlsband CA-USA) supplemented with 10% FBS (Invitrogen). .. MPCs were isolated from BMMNCs cultured in DMEM supplemented with 10% pooled human AB serum, obtained from male donors only (PhABS, Lonza, Walkersville MD-USA), as previously described . .. Media were changed every 48 h and cultures maintained at 37°C and 5% CO2 for 10–12 days, then detached by TrypLE Select® (Invitrogen) digestion and processed for characterization and mRNA extraction.

    Isolation:

    Article Title: Angiogenic Mechanisms of Human CD34+ Stem Cell Exosomes in the Repair of Ischemic Hindlimb
    Article Snippet: The cells were purified from mobilized peripheral-blood mononuclear cells (AllCells LLC, Emeryville, CA, USA) with an Isolex 300i device (Baxter Healthcare); cell purity (85–95%) was determined via flow cytometry. .. Both CD34+ cells and MNCs (250,000 cells/mL) were cultured in X-VIVO 10 serum-free cell-culture medium (Lonza Inc. Allendale, NJ) and ultra-pure exosomes were isolated from the conditioned media by ultracentrifugation and separated from the soluble protein fraction on a sucrose gradient . .. The exosomes from both cell types were characterized using dynamic light scattering, flow cytometery analysis, electron microscopy and immunoblotting as described before , .

    Article Title: S100A9-induced overexpression of PD-1/PD-L1 contributes to ineffective hematopoiesis in myelodysplastic syndromes
    Article Snippet: .. BM-mononuclear cells (MNC) were isolated from heparinized MDS BM and from heparinized BM of healthy human donors (purchased from Lonza-Walkersville). ..

    Article Title: S100A9-induced overexpression of PD-1/PD-L1 contributes to ineffective hematopoiesis in myelodysplastic syndromes
    Article Snippet: .. BM-MNC were isolated from heparinized MDS BM and from heparinized BM of healthy human donors (purchased from Lonza-Walkersville). ..

    Article Title: Mesodermal Progenitor Cells (MPCs) Differentiate into Mesenchymal Stromal Cells (MSCs) by Activation of Wnt5/Calmodulin Signalling Pathway
    Article Snippet: MSC cultures were obtained from bone marrow mononuclear cells (BMMNCs) grown under standard conditions, using DMEM (Invitrogen, Carlsband CA-USA) supplemented with 10% FBS (Invitrogen). .. MPCs were isolated from BMMNCs cultured in DMEM supplemented with 10% pooled human AB serum, obtained from male donors only (PhABS, Lonza, Walkersville MD-USA), as previously described . .. Media were changed every 48 h and cultures maintained at 37°C and 5% CO2 for 10–12 days, then detached by TrypLE Select® (Invitrogen) digestion and processed for characterization and mRNA extraction.

    FACS:

    Article Title: NG2+/Nestin+ mesenchymal stem cells dictate DTC dormancy in the bone marrow through TGFβ2
    Article Snippet: Bone marrow (BM) cells were flushed from femurs and tibias, red-blood-cell lysis buffer (Lonza) was used for 2 minutes followed by quantification of the GFP+ cells and normalization to the total number of BM cells. .. Flow cytometry and cell sorting Bone marrow cells were flushed, incubated in red-blood cell lysis buffer (Lonza) for 2 minutes and remaining cells were permeabilized with 0.05% Triton (when using intracellular antibodies) and stained using antibodies and conditions in . .. All experiments were performed using BD FACSAria II equipped with FACS Diva software (BD Biosciences).

    Incubation:

    Article Title: NG2+/Nestin+ mesenchymal stem cells dictate DTC dormancy in the bone marrow through TGFβ2
    Article Snippet: Bone marrow (BM) cells were flushed from femurs and tibias, red-blood-cell lysis buffer (Lonza) was used for 2 minutes followed by quantification of the GFP+ cells and normalization to the total number of BM cells. .. Flow cytometry and cell sorting Bone marrow cells were flushed, incubated in red-blood cell lysis buffer (Lonza) for 2 minutes and remaining cells were permeabilized with 0.05% Triton (when using intracellular antibodies) and stained using antibodies and conditions in . .. All experiments were performed using BD FACSAria II equipped with FACS Diva software (BD Biosciences).

    Lysis:

    Article Title: NG2+/Nestin+ mesenchymal stem cells dictate DTC dormancy in the bone marrow through TGFβ2
    Article Snippet: Bone marrow (BM) cells were flushed from femurs and tibias, red-blood-cell lysis buffer (Lonza) was used for 2 minutes followed by quantification of the GFP+ cells and normalization to the total number of BM cells. .. Flow cytometry and cell sorting Bone marrow cells were flushed, incubated in red-blood cell lysis buffer (Lonza) for 2 minutes and remaining cells were permeabilized with 0.05% Triton (when using intracellular antibodies) and stained using antibodies and conditions in . .. All experiments were performed using BD FACSAria II equipped with FACS Diva software (BD Biosciences).

    Staining:

    Article Title: NG2+/Nestin+ mesenchymal stem cells dictate DTC dormancy in the bone marrow through TGFβ2
    Article Snippet: Bone marrow (BM) cells were flushed from femurs and tibias, red-blood-cell lysis buffer (Lonza) was used for 2 minutes followed by quantification of the GFP+ cells and normalization to the total number of BM cells. .. Flow cytometry and cell sorting Bone marrow cells were flushed, incubated in red-blood cell lysis buffer (Lonza) for 2 minutes and remaining cells were permeabilized with 0.05% Triton (when using intracellular antibodies) and stained using antibodies and conditions in . .. All experiments were performed using BD FACSAria II equipped with FACS Diva software (BD Biosciences).

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    • mncs  (Lonza)
    94
    Lonza mncs
    CD34Exo carry pro-angiogenic miRNA. Human angiogenesis protein array comparing CD34Exo and MNCExo ( A ); quantification of proteins present in CD34Exo and MNCExo using human angiogenesis protein array ( B ); miRNA expression profiles comparing <t>CD34</t> + cells with <t>MNCs</t> and CD34Exo with MNCExo, shown using heat map expression analysis ( C ).
    Mncs, supplied by Lonza, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mncs/product/Lonza
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mncs - by Bioz Stars, 2021-03
    94/100 stars
    		  Buy from Supplier
    peripheral blood mncs  (Lonza)
    94
    Lonza peripheral blood mncs
    The representative images of eEPCs (A–C) and <t>ECFCs</t> (D–F) derived from peripheral blood <t>MNCs</t> obtained from healthy and diabetic individuals. Images C and D , and E and F , were derived from two different diabetic individuals, respectively, and showed inconsistency in the generation of eEPCs and ECFCs. Scale bar measures 200 microns.
    Peripheral Blood Mncs, supplied by Lonza, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/peripheral blood mncs/product/Lonza
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    peripheral blood mncs - by Bioz Stars, 2021-03
    94/100 stars
    		  Buy from Supplier

    Image Search Results


    CD34Exo carry pro-angiogenic miRNA. Human angiogenesis protein array comparing CD34Exo and MNCExo ( A ); quantification of proteins present in CD34Exo and MNCExo using human angiogenesis protein array ( B ); miRNA expression profiles comparing CD34 + cells with MNCs and CD34Exo with MNCExo, shown using heat map expression analysis ( C ).

    Journal: Circulation research

    Article Title: Angiogenic Mechanisms of Human CD34+ Stem Cell Exosomes in the Repair of Ischemic Hindlimb

    doi: 10.1161/CIRCRESAHA.116.310557

    Figure Lengend Snippet: CD34Exo carry pro-angiogenic miRNA. Human angiogenesis protein array comparing CD34Exo and MNCExo ( A ); quantification of proteins present in CD34Exo and MNCExo using human angiogenesis protein array ( B ); miRNA expression profiles comparing CD34 + cells with MNCs and CD34Exo with MNCExo, shown using heat map expression analysis ( C ).

    Article Snippet: Both CD34+ cells and MNCs (250,000 cells/mL) were cultured in X-VIVO 10 serum-free cell-culture medium (Lonza Inc. Allendale, NJ) and ultra-pure exosomes were isolated from the conditioned media by ultracentrifugation and separated from the soluble protein fraction on a sucrose gradient .

    Techniques: Protein Array, Expressing

    Characterisation of the TLR-antagonistic effects of TIP3 on primary cells. (a) TIP3 was found to be safe for THP-1 cells at multiple concentrations (≤ 50 μM) for 24 h. (b, c) The protein expression was measured by western blot analyses using hPBMCs. The amounts of p-p65, Iκ-Bα, p-IRF3, ATF3, p-ERK, ERK, p-JNK, JNK, p-p38 and p38 were evaluated in the total-protein extract. β-Actin served as a loading control. (d, e) The secretion levels of TNF-α and IL-6 were measured by ELISA after the cells were stimulated with LPS and then treated with TIP3. (f–i) The effects of TIP3 on the LPS-induced mRNA expression levels of IL-6, TNF-α, IL-8, and IL-1β after 4 h. The mRNA levels of these genes were substantially downregulated by TIP3. Values are indicated as fold changes (relative quantification; RQ) in mRNA levels, normalized to GAPDH. (j–m) The LPS-stimulated mBMDMs were treated with TIP3 for 1 h and the secretion levels of (j) IL-6 and (k) TNF-α were measured by an ELISA, and (m) the production of NO was evaluated using NO secretion kit. (l) The, IFN-β level was measured in poly(I:C)-stimulated mBMDMs through ELISA. (n-p) The LPS-stimulated hMNCs were also utilized to evaluate the secretion levels of (o) IL-6 and (p) TNF-α by ELISA. (q) The poly (I:C)-stimulated hMNCs were employed to assess the TLR3-inhibitory effect of TIP3 by measuring the TNF-α secretion by an ELISA. Effects similar to those of in RAW264.7 cells were detected. The data shown represent at least three independent experiments ( n ≥ 3), and bars denote mean ± SEM (* P

    Journal: EBioMedicine

    Article Title: A peptide derived from the core β-sheet region of TIRAP decoys TLR4 and reduces inflammatory and autoimmune symptoms in murine models

    doi: 10.1016/j.ebiom.2020.102645

    Figure Lengend Snippet: Characterisation of the TLR-antagonistic effects of TIP3 on primary cells. (a) TIP3 was found to be safe for THP-1 cells at multiple concentrations (≤ 50 μM) for 24 h. (b, c) The protein expression was measured by western blot analyses using hPBMCs. The amounts of p-p65, Iκ-Bα, p-IRF3, ATF3, p-ERK, ERK, p-JNK, JNK, p-p38 and p38 were evaluated in the total-protein extract. β-Actin served as a loading control. (d, e) The secretion levels of TNF-α and IL-6 were measured by ELISA after the cells were stimulated with LPS and then treated with TIP3. (f–i) The effects of TIP3 on the LPS-induced mRNA expression levels of IL-6, TNF-α, IL-8, and IL-1β after 4 h. The mRNA levels of these genes were substantially downregulated by TIP3. Values are indicated as fold changes (relative quantification; RQ) in mRNA levels, normalized to GAPDH. (j–m) The LPS-stimulated mBMDMs were treated with TIP3 for 1 h and the secretion levels of (j) IL-6 and (k) TNF-α were measured by an ELISA, and (m) the production of NO was evaluated using NO secretion kit. (l) The, IFN-β level was measured in poly(I:C)-stimulated mBMDMs through ELISA. (n-p) The LPS-stimulated hMNCs were also utilized to evaluate the secretion levels of (o) IL-6 and (p) TNF-α by ELISA. (q) The poly (I:C)-stimulated hMNCs were employed to assess the TLR3-inhibitory effect of TIP3 by measuring the TNF-α secretion by an ELISA. Effects similar to those of in RAW264.7 cells were detected. The data shown represent at least three independent experiments ( n ≥ 3), and bars denote mean ± SEM (* P

    Article Snippet: Human peripheral blood mononuclear cells (hPBMCs) and human mononuclear cells (hMNCs) were purchased from Lonza Inc. (Allendale, NJ, USA) and cultured in RPMI 1640 containing 2·05 mM l -glutamine, 1% of the penicillin/streptomycin solution, and 10% of FBS.

    Techniques: Expressing, Western Blot, Enzyme-linked Immunosorbent Assay

    TRIF and TRAF6 are degraded through non-canonical autophagy. a HeLa cells were transfected with A20 siRNA for 24 h. Cells were pretreated with or without 25 μM Z-VAD-fmk for 1 h and then stimulated with 50 μg/ml poly (I:C) for the indicated periods. Cell lysates were analyzed by IB with antibodies to TRIF , TRAF6 , TRAF3 and GAPDH . b HeLa cells were transfected with A20 siRNA for 24 h. Cells were pretreated with either 20 μM MG-132 or 10 mM 3-MA for 3 h and then stimulated with 50 μg/ml poly (I:C) for 6 h. Cell lysates were analyzed by IB with antibodies to TRIF , TRAF6 , TRAF3 and GAPDH . c HEK293T cells were transfected with Flag-TRIF for 18 h. Cells were then treated with either 20 μM MG-132 or 10 mM 3-MA for 6 h. Cell lysates were analyzed by IB with antibodies to Flag epitope, TRAF6 , TRAF3 and GAPDH . d HeLa cells were transfected with A20 siRNA for 24 h. Cells were pretreated with 30 μM E-64, 30 μM Pepstatin A, 30 μM Leupeptin or 100 nM Bafilomycin A1 for 4 h. Then cells were stimulated with 50 μg/ml poly (I:C) for 6 h. Cell lysates were analyzed by IB with antibodies to TRIF , TRAF6 , TRAF3 and GAPDH . e Human bone marrow-derived primary mononuclear cells were transfected with A20 siRNA and maintained in medium supplemented with 50 ng/ml M-CSF for 48 h. Cells were stimulated with 50 μg/ml poly(I:C) for 6 h. Then the cells were assessed by electron microscopy. Arrows autophagic vacuoles. Arrowheads autophagosomes. The magnified photo represents the area indicated by the square . For each cell, the autophagic area was calculated by expressing the total area of autophagic vacuoles as a percentage of the cytoplasmic area. Scale bar 2 μm. Original magnification, ×3,000. Error bars ±SD ( n = 5). * P ≤ 0.01. f HeLa cells were transfected with control siRNA or A20 siRNA for 24 h. Cells were then stimulated with 50 μg/ml poly (I:C) for 6 h. Cell lysates were analyzed by IB with antibodies to LC3 , GAPDH and A20 . g HeLa cells were cotransfected with A20 siRNA and GFP-LC3 for 24 h. Cells were then stimulated with 50 μg/ml poly (I:C) for 6 h. Cell lysates were analyzed by IB with antibodies to GFP and GAPDH . h HeLa cells were transfected with A20 siRNA together with control siRNA or Beclin-1 siRNA for 24 h. Cells were then stimulated with 50 μg/ml poly (I:C) for the indicated periods. Cell lysates were analyzed by IB with antibodies to TRIF , TRAF6 , TRAF3 , Beclin- 1 and GAPDH

    Journal: Cellular and Molecular Life Sciences

    Article Title: Regulation of Toll-like receptor signaling by NDP52-mediated selective autophagy is normally inactivated by A20

    doi: 10.1007/s00018-011-0819-y

    Figure Lengend Snippet: TRIF and TRAF6 are degraded through non-canonical autophagy. a HeLa cells were transfected with A20 siRNA for 24 h. Cells were pretreated with or without 25 μM Z-VAD-fmk for 1 h and then stimulated with 50 μg/ml poly (I:C) for the indicated periods. Cell lysates were analyzed by IB with antibodies to TRIF , TRAF6 , TRAF3 and GAPDH . b HeLa cells were transfected with A20 siRNA for 24 h. Cells were pretreated with either 20 μM MG-132 or 10 mM 3-MA for 3 h and then stimulated with 50 μg/ml poly (I:C) for 6 h. Cell lysates were analyzed by IB with antibodies to TRIF , TRAF6 , TRAF3 and GAPDH . c HEK293T cells were transfected with Flag-TRIF for 18 h. Cells were then treated with either 20 μM MG-132 or 10 mM 3-MA for 6 h. Cell lysates were analyzed by IB with antibodies to Flag epitope, TRAF6 , TRAF3 and GAPDH . d HeLa cells were transfected with A20 siRNA for 24 h. Cells were pretreated with 30 μM E-64, 30 μM Pepstatin A, 30 μM Leupeptin or 100 nM Bafilomycin A1 for 4 h. Then cells were stimulated with 50 μg/ml poly (I:C) for 6 h. Cell lysates were analyzed by IB with antibodies to TRIF , TRAF6 , TRAF3 and GAPDH . e Human bone marrow-derived primary mononuclear cells were transfected with A20 siRNA and maintained in medium supplemented with 50 ng/ml M-CSF for 48 h. Cells were stimulated with 50 μg/ml poly(I:C) for 6 h. Then the cells were assessed by electron microscopy. Arrows autophagic vacuoles. Arrowheads autophagosomes. The magnified photo represents the area indicated by the square . For each cell, the autophagic area was calculated by expressing the total area of autophagic vacuoles as a percentage of the cytoplasmic area. Scale bar 2 μm. Original magnification, ×3,000. Error bars ±SD ( n = 5). * P ≤ 0.01. f HeLa cells were transfected with control siRNA or A20 siRNA for 24 h. Cells were then stimulated with 50 μg/ml poly (I:C) for 6 h. Cell lysates were analyzed by IB with antibodies to LC3 , GAPDH and A20 . g HeLa cells were cotransfected with A20 siRNA and GFP-LC3 for 24 h. Cells were then stimulated with 50 μg/ml poly (I:C) for 6 h. Cell lysates were analyzed by IB with antibodies to GFP and GAPDH . h HeLa cells were transfected with A20 siRNA together with control siRNA or Beclin-1 siRNA for 24 h. Cells were then stimulated with 50 μg/ml poly (I:C) for the indicated periods. Cell lysates were analyzed by IB with antibodies to TRIF , TRAF6 , TRAF3 , Beclin- 1 and GAPDH

    Article Snippet: Human bone marrow-derived primary mononuclear cells were obtained from Lonza (2M-125C).

    Techniques: Transfection, FLAG-tag, Derivative Assay, Electron Microscopy, Expressing

    The representative images of eEPCs (A–C) and ECFCs (D–F) derived from peripheral blood MNCs obtained from healthy and diabetic individuals. Images C and D , and E and F , were derived from two different diabetic individuals, respectively, and showed inconsistency in the generation of eEPCs and ECFCs. Scale bar measures 200 microns.

    Journal: PLoS ONE

    Article Title: Vasoreparative Dysfunction of CD34+ Cells in Diabetic Individuals Involves Hypoxic Desensitization and Impaired Autocrine/Paracrine Mechanisms

    doi: 10.1371/journal.pone.0093965

    Figure Lengend Snippet: The representative images of eEPCs (A–C) and ECFCs (D–F) derived from peripheral blood MNCs obtained from healthy and diabetic individuals. Images C and D , and E and F , were derived from two different diabetic individuals, respectively, and showed inconsistency in the generation of eEPCs and ECFCs. Scale bar measures 200 microns.

    Article Snippet: Culturing ECFCs and eEPCs For ECFCs, peripheral blood MNCs were suspended in EBM-2 medium supplemented with EGM-2MV single quotes (Lonza).

    Techniques: Derivative Assay