Structured Review

Diagenode mnase
Mnase, supplied by Diagenode, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mnase/product/Diagenode
Average 92 stars, based on 1 article reviews
Price from $9.99 to $1999.99
mnase - by Bioz Stars, 2020-05
92/100 stars

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Related Articles

Isolation:

Article Title: Ezh2 Regulates Activation-Induced CD8+ T Cell Cycle Progression via Repressing Cdkn2a and Cdkn1c Expression
Article Snippet: .. Chromatin Immunoprecipitation Naïve CD8+ T cells were isolated freshly and stimulated with anti-CD3/CD28 in vitro for 72 h. The cells were digested by 0.2U MNase per million cells for 10 min and sonicated (Diagenode) with a power setting of 5 for 4 repeats of 30 s followed by 30 s break between each repeat in ice-cold water to obtain the average size between 100 and 300 bps. .. The sonicated products were dialyzed with 10000 MWCO Cassette G2 (Thermo-Fisher) in 400 ml RIPA buffer for 2 h at 4°C and then incubated overnight with 2 µg anti-H3K27me3 antibody (Millipore, 07-449) or IgG-conjugated Dynabeads Protein G (Thermo-Fisher).

Chromatin Immunoprecipitation:

Article Title: Ezh2 Regulates Activation-Induced CD8+ T Cell Cycle Progression via Repressing Cdkn2a and Cdkn1c Expression
Article Snippet: .. Chromatin Immunoprecipitation Naïve CD8+ T cells were isolated freshly and stimulated with anti-CD3/CD28 in vitro for 72 h. The cells were digested by 0.2U MNase per million cells for 10 min and sonicated (Diagenode) with a power setting of 5 for 4 repeats of 30 s followed by 30 s break between each repeat in ice-cold water to obtain the average size between 100 and 300 bps. .. The sonicated products were dialyzed with 10000 MWCO Cassette G2 (Thermo-Fisher) in 400 ml RIPA buffer for 2 h at 4°C and then incubated overnight with 2 µg anti-H3K27me3 antibody (Millipore, 07-449) or IgG-conjugated Dynabeads Protein G (Thermo-Fisher).

In Vitro:

Article Title: Ezh2 Regulates Activation-Induced CD8+ T Cell Cycle Progression via Repressing Cdkn2a and Cdkn1c Expression
Article Snippet: .. Chromatin Immunoprecipitation Naïve CD8+ T cells were isolated freshly and stimulated with anti-CD3/CD28 in vitro for 72 h. The cells were digested by 0.2U MNase per million cells for 10 min and sonicated (Diagenode) with a power setting of 5 for 4 repeats of 30 s followed by 30 s break between each repeat in ice-cold water to obtain the average size between 100 and 300 bps. .. The sonicated products were dialyzed with 10000 MWCO Cassette G2 (Thermo-Fisher) in 400 ml RIPA buffer for 2 h at 4°C and then incubated overnight with 2 µg anti-H3K27me3 antibody (Millipore, 07-449) or IgG-conjugated Dynabeads Protein G (Thermo-Fisher).

Sonication:

Article Title: Ezh2 Regulates Activation-Induced CD8+ T Cell Cycle Progression via Repressing Cdkn2a and Cdkn1c Expression
Article Snippet: .. Chromatin Immunoprecipitation Naïve CD8+ T cells were isolated freshly and stimulated with anti-CD3/CD28 in vitro for 72 h. The cells were digested by 0.2U MNase per million cells for 10 min and sonicated (Diagenode) with a power setting of 5 for 4 repeats of 30 s followed by 30 s break between each repeat in ice-cold water to obtain the average size between 100 and 300 bps. .. The sonicated products were dialyzed with 10000 MWCO Cassette G2 (Thermo-Fisher) in 400 ml RIPA buffer for 2 h at 4°C and then incubated overnight with 2 µg anti-H3K27me3 antibody (Millipore, 07-449) or IgG-conjugated Dynabeads Protein G (Thermo-Fisher).

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    Diagenode mnase
    Mnase, supplied by Diagenode, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mnase/product/Diagenode
    Average 92 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    mnase - by Bioz Stars, 2020-05
    92/100 stars
      Buy from Supplier

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