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1) Product Images from "Different roles for Abf1p and a T-rich promoter element in nucleosome organization of the yeast RPS28A gene"
Article Title: Different roles for Abf1p and a T-rich promoter element in nucleosome organization of the yeast RPS28A gene
Journal: Nucleic Acids Research
Figure Legend Snippet: Comparison of in vivo nucleosome positions on the wild-type RPS28A promoter and a promoter containing a mutated T-rich element. Nystatin-permeabilized spheroplasts prepared from wild-type (ERS3) and mutant cells (ETS6) were treated with either Mnase ( A ) or DNaseI ( B ). Distances are from the translational start codon ATG. Protected regions of 160–170 bp are interpreted as positioned nucleosomes (closed elipses). The 130 bp Mnase-protected region that covers the Abf1p site ( Xba I) in the wild-type promoter (ERS3) has decreased to 85 bp when the T-rich element is mutated (ERS6). Concomitantly, downstream nucleosomes are shifted towards the Abf1p binding site.
Techniques Used: In Vivo, Mutagenesis, Binding Assay