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Boehringer Mannheim mnase
Comparison of in vivo nucleosome positions on the wild-type RPS28A promoter and a promoter containing a mutated T-rich element. Nystatin-permeabilized spheroplasts prepared from wild-type (ERS3) and mutant cells (ETS6) were treated with either <t>Mnase</t> ( A ) or <t>DNaseI</t> ( B ). Distances are from the translational start codon ATG. Protected regions of 160–170 bp are interpreted as positioned nucleosomes (closed elipses). The 130 bp Mnase-protected region that covers the Abf1p site ( Xba I) in the wild-type promoter (ERS3) has decreased to 85 bp when the T-rich element is mutated (ERS6). Concomitantly, downstream nucleosomes are shifted towards the Abf1p binding site.
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Article Title: Different roles for Abf1p and a T-rich promoter element in nucleosome organization of the yeast RPS28A gene

Journal: Nucleic Acids Research

doi:

Comparison of in vivo nucleosome positions on the wild-type RPS28A promoter and a promoter containing a mutated T-rich element. Nystatin-permeabilized spheroplasts prepared from wild-type (ERS3) and mutant cells (ETS6) were treated with either Mnase ( A ) or DNaseI ( B ). Distances are from the translational start codon ATG. Protected regions of 160–170 bp are interpreted as positioned nucleosomes (closed elipses). The 130 bp Mnase-protected region that covers the Abf1p site ( Xba I) in the wild-type promoter (ERS3) has decreased to 85 bp when the T-rich element is mutated (ERS6). Concomitantly, downstream nucleosomes are shifted towards the Abf1p binding site.
Figure Legend Snippet: Comparison of in vivo nucleosome positions on the wild-type RPS28A promoter and a promoter containing a mutated T-rich element. Nystatin-permeabilized spheroplasts prepared from wild-type (ERS3) and mutant cells (ETS6) were treated with either Mnase ( A ) or DNaseI ( B ). Distances are from the translational start codon ATG. Protected regions of 160–170 bp are interpreted as positioned nucleosomes (closed elipses). The 130 bp Mnase-protected region that covers the Abf1p site ( Xba I) in the wild-type promoter (ERS3) has decreased to 85 bp when the T-rich element is mutated (ERS6). Concomitantly, downstream nucleosomes are shifted towards the Abf1p binding site.

Techniques Used: In Vivo, Mutagenesis, Binding Assay

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    Boehringer Mannheim mnase
    Comparison of in vivo nucleosome positions on the wild-type RPS28A promoter and a promoter containing a mutated T-rich element. Nystatin-permeabilized spheroplasts prepared from wild-type (ERS3) and mutant cells (ETS6) were treated with either <t>Mnase</t> ( A ) or <t>DNaseI</t> ( B ). Distances are from the translational start codon ATG. Protected regions of 160–170 bp are interpreted as positioned nucleosomes (closed elipses). The 130 bp Mnase-protected region that covers the Abf1p site ( Xba I) in the wild-type promoter (ERS3) has decreased to 85 bp when the T-rich element is mutated (ERS6). Concomitantly, downstream nucleosomes are shifted towards the Abf1p binding site.
    Mnase, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mnase/product/Boehringer Mannheim
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mnase - by Bioz Stars, 2020-05
    92/100 stars
      Buy from Supplier

    90
    Boehringer Mannheim micrococcal nuclease mnase
    Comparison of in vivo nucleosome positions on the wild-type RPS28A promoter and a promoter containing a mutated T-rich element. Nystatin-permeabilized spheroplasts prepared from wild-type (ERS3) and mutant cells (ETS6) were treated with either <t>Mnase</t> ( A ) or <t>DNaseI</t> ( B ). Distances are from the translational start codon ATG. Protected regions of 160–170 bp are interpreted as positioned nucleosomes (closed elipses). The 130 bp Mnase-protected region that covers the Abf1p site ( Xba I) in the wild-type promoter (ERS3) has decreased to 85 bp when the T-rich element is mutated (ERS6). Concomitantly, downstream nucleosomes are shifted towards the Abf1p binding site.
    Micrococcal Nuclease Mnase, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    micrococcal nuclease mnase - by Bioz Stars, 2020-05
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    Boehringer Mannheim s7 micrococcal nuclease
    Comparison of in vivo nucleosome positions on the wild-type RPS28A promoter and a promoter containing a mutated T-rich element. Nystatin-permeabilized spheroplasts prepared from wild-type (ERS3) and mutant cells (ETS6) were treated with either <t>Mnase</t> ( A ) or <t>DNaseI</t> ( B ). Distances are from the translational start codon ATG. Protected regions of 160–170 bp are interpreted as positioned nucleosomes (closed elipses). The 130 bp Mnase-protected region that covers the Abf1p site ( Xba I) in the wild-type promoter (ERS3) has decreased to 85 bp when the T-rich element is mutated (ERS6). Concomitantly, downstream nucleosomes are shifted towards the Abf1p binding site.
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    Comparison of in vivo nucleosome positions on the wild-type RPS28A promoter and a promoter containing a mutated T-rich element. Nystatin-permeabilized spheroplasts prepared from wild-type (ERS3) and mutant cells (ETS6) were treated with either Mnase ( A ) or DNaseI ( B ). Distances are from the translational start codon ATG. Protected regions of 160–170 bp are interpreted as positioned nucleosomes (closed elipses). The 130 bp Mnase-protected region that covers the Abf1p site ( Xba I) in the wild-type promoter (ERS3) has decreased to 85 bp when the T-rich element is mutated (ERS6). Concomitantly, downstream nucleosomes are shifted towards the Abf1p binding site.

    Journal: Nucleic Acids Research

    Article Title: Different roles for Abf1p and a T-rich promoter element in nucleosome organization of the yeast RPS28A gene

    doi:

    Figure Lengend Snippet: Comparison of in vivo nucleosome positions on the wild-type RPS28A promoter and a promoter containing a mutated T-rich element. Nystatin-permeabilized spheroplasts prepared from wild-type (ERS3) and mutant cells (ETS6) were treated with either Mnase ( A ) or DNaseI ( B ). Distances are from the translational start codon ATG. Protected regions of 160–170 bp are interpreted as positioned nucleosomes (closed elipses). The 130 bp Mnase-protected region that covers the Abf1p site ( Xba I) in the wild-type promoter (ERS3) has decreased to 85 bp when the T-rich element is mutated (ERS6). Concomitantly, downstream nucleosomes are shifted towards the Abf1p binding site.

    Article Snippet: For chromatin samples, after the 0 min time sample, either 250 U/4 ml Mnase (micrococcal endonuclease) or 180 U/4 ml DNaseI (both from Boehringer-Mannheim) was added to the nystatin-permeabilized spheroplasts, and 500 µl time samples (1, 2.5, 5, 9, 15 and 25 min) were added to 50 µl StopMnase (10% SDS, 0.1 M EDTA and 50 µg proteinase K).

    Techniques: In Vivo, Mutagenesis, Binding Assay