Structured Review

Millipore mnase i
Mnase I, supplied by Millipore, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mnase i/product/Millipore
Average 88 stars, based on 1 article reviews
Price from $9.99 to $1999.99
mnase i - by Bioz Stars, 2020-08
88/100 stars

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Related Articles

Staining:

Article Title: The Interaction of NSBP1/HMGN5 with Nucleosomes in Euchromatin Counteracts Linker Histone-Mediated Chromatin Compaction and Modulates Transcription
Article Snippet: .. After 48 hr, the cells were washed and the nuclei isolated and digested with 30 units of MNase I (Sigma) for 1, 2, 4, 8, 16, and 24 min. Isolated DNA were analyzed by 1.5% TAE agarose gel and ethidium bromide staining. .. Stable AtT20 clones overexpressing NSBP1-FLHA, NSBP1S17/121E FLHA, or control empty retroviral vector and AtT20 cells treated with specific or control siRNA were collected for RNA extraction.

Centrifugation:

Article Title: Defective histone supply causes changes in RNA polymerase II elongation rate and cotranscriptional pre-mRNA splicing
Article Snippet: .. Nuclei were obtained after centrifugation and were digested with 2 U of MNase I (Sigma) per 4 × 106 nuclei for 2 or 4 min at room temperature in buffer A supplemented with 10 mM CaCl2 . .. After DNA purification, samples were run in a 1.2% agarose gel, and densitometry was determined in a Fuji FLA5100 fluorescent image analyzer with the Image Gauge analysis program.

Agarose Gel Electrophoresis:

Article Title: The Interaction of NSBP1/HMGN5 with Nucleosomes in Euchromatin Counteracts Linker Histone-Mediated Chromatin Compaction and Modulates Transcription
Article Snippet: .. After 48 hr, the cells were washed and the nuclei isolated and digested with 30 units of MNase I (Sigma) for 1, 2, 4, 8, 16, and 24 min. Isolated DNA were analyzed by 1.5% TAE agarose gel and ethidium bromide staining. .. Stable AtT20 clones overexpressing NSBP1-FLHA, NSBP1S17/121E FLHA, or control empty retroviral vector and AtT20 cells treated with specific or control siRNA were collected for RNA extraction.

Isolation:

Article Title: The Interaction of NSBP1/HMGN5 with Nucleosomes in Euchromatin Counteracts Linker Histone-Mediated Chromatin Compaction and Modulates Transcription
Article Snippet: .. After 48 hr, the cells were washed and the nuclei isolated and digested with 30 units of MNase I (Sigma) for 1, 2, 4, 8, 16, and 24 min. Isolated DNA were analyzed by 1.5% TAE agarose gel and ethidium bromide staining. .. Stable AtT20 clones overexpressing NSBP1-FLHA, NSBP1S17/121E FLHA, or control empty retroviral vector and AtT20 cells treated with specific or control siRNA were collected for RNA extraction.

Concentration Assay:

Article Title: UpSET recruits HDAC complexes and restricts chromatin accessibility and histone acetylation at promoter regions
Article Snippet: .. Nuclei were treated with MNase I (1.6 U/ml, Sigma) for 10min at 37°C and the reaction stopped by adding EDTA to a final 20mM concentration. ..

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  • 99
    Millipore rapamycin
    IFN-γ suppresses mTORC1 activation and downstream functions ( a-c ) Immunoblot analysis of whole cell lysates from control or IFN-γ-treated macrophages stimulated with Pam3CSK4 (10 ng/ml) for the indicated times and probed with antibodies against p-4E-BP1 ( a , b ) or p-p70S6K ( c ). In ( b ), mTOR inhibitors PP242 (50 nM ), Torin1 (50 nM) or <t>Rapamycin</t> (500 nM) were added for 30 min. ( d ) Immunoblot analysis of LC3A and LC3B in control or IFN-γ-primed macrophages. ( e ) Upper: Immunofluorescence images of LAMP1 (red) and mTOR (green) co-staining in control or IFN-γ-primed macrophages stimulated with Pam3CSK4 (10 ng/ml) for 4 h; nuclei were stained with DAPI (blue). Quantitation of co-localization (lower panel) between LAMP1 and mTOR; data are presented as mean ± SEM of the percentage of co-localized cells from 600 cells analyzed in three independent experiments; * p = 0.0001 by unpaired student t test. ( f ) Immunoblot analysis of HES1 in human primary macrophages pretreated for 30min with vehicle control DMSO or increasing concentrations of Rapamycin (0 nM, 500 nM, 1 μM), and then stimulated with Pam3CSK4 (10 ng/ml) for 0, 2, or 4h; p38α serves as loading control. Data are representative of at least three independent experiments ( a-e ).
    Rapamycin, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 458 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Millipore lysine 4
    IFN-γ suppresses mTORC1 activation and downstream functions ( a-c ) Immunoblot analysis of whole cell lysates from control or IFN-γ-treated macrophages stimulated with Pam3CSK4 (10 ng/ml) for the indicated times and probed with antibodies against p-4E-BP1 ( a , b ) or p-p70S6K ( c ). In ( b ), mTOR inhibitors PP242 (50 nM ), Torin1 (50 nM) or <t>Rapamycin</t> (500 nM) were added for 30 min. ( d ) Immunoblot analysis of LC3A and LC3B in control or IFN-γ-primed macrophages. ( e ) Upper: Immunofluorescence images of LAMP1 (red) and mTOR (green) co-staining in control or IFN-γ-primed macrophages stimulated with Pam3CSK4 (10 ng/ml) for 4 h; nuclei were stained with DAPI (blue). Quantitation of co-localization (lower panel) between LAMP1 and mTOR; data are presented as mean ± SEM of the percentage of co-localized cells from 600 cells analyzed in three independent experiments; * p = 0.0001 by unpaired student t test. ( f ) Immunoblot analysis of HES1 in human primary macrophages pretreated for 30min with vehicle control DMSO or increasing concentrations of Rapamycin (0 nM, 500 nM, 1 μM), and then stimulated with Pam3CSK4 (10 ng/ml) for 0, 2, or 4h; p38α serves as loading control. Data are representative of at least three independent experiments ( a-e ).
    Lysine 4, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Millipore antiphospho h2ax
    IFN-γ suppresses mTORC1 activation and downstream functions ( a-c ) Immunoblot analysis of whole cell lysates from control or IFN-γ-treated macrophages stimulated with Pam3CSK4 (10 ng/ml) for the indicated times and probed with antibodies against p-4E-BP1 ( a , b ) or p-p70S6K ( c ). In ( b ), mTOR inhibitors PP242 (50 nM ), Torin1 (50 nM) or <t>Rapamycin</t> (500 nM) were added for 30 min. ( d ) Immunoblot analysis of LC3A and LC3B in control or IFN-γ-primed macrophages. ( e ) Upper: Immunofluorescence images of LAMP1 (red) and mTOR (green) co-staining in control or IFN-γ-primed macrophages stimulated with Pam3CSK4 (10 ng/ml) for 4 h; nuclei were stained with DAPI (blue). Quantitation of co-localization (lower panel) between LAMP1 and mTOR; data are presented as mean ± SEM of the percentage of co-localized cells from 600 cells analyzed in three independent experiments; * p = 0.0001 by unpaired student t test. ( f ) Immunoblot analysis of HES1 in human primary macrophages pretreated for 30min with vehicle control DMSO or increasing concentrations of Rapamycin (0 nM, 500 nM, 1 μM), and then stimulated with Pam3CSK4 (10 ng/ml) for 0, 2, or 4h; p38α serves as loading control. Data are representative of at least three independent experiments ( a-e ).
    Antiphospho H2ax, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    IFN-γ suppresses mTORC1 activation and downstream functions ( a-c ) Immunoblot analysis of whole cell lysates from control or IFN-γ-treated macrophages stimulated with Pam3CSK4 (10 ng/ml) for the indicated times and probed with antibodies against p-4E-BP1 ( a , b ) or p-p70S6K ( c ). In ( b ), mTOR inhibitors PP242 (50 nM ), Torin1 (50 nM) or Rapamycin (500 nM) were added for 30 min. ( d ) Immunoblot analysis of LC3A and LC3B in control or IFN-γ-primed macrophages. ( e ) Upper: Immunofluorescence images of LAMP1 (red) and mTOR (green) co-staining in control or IFN-γ-primed macrophages stimulated with Pam3CSK4 (10 ng/ml) for 4 h; nuclei were stained with DAPI (blue). Quantitation of co-localization (lower panel) between LAMP1 and mTOR; data are presented as mean ± SEM of the percentage of co-localized cells from 600 cells analyzed in three independent experiments; * p = 0.0001 by unpaired student t test. ( f ) Immunoblot analysis of HES1 in human primary macrophages pretreated for 30min with vehicle control DMSO or increasing concentrations of Rapamycin (0 nM, 500 nM, 1 μM), and then stimulated with Pam3CSK4 (10 ng/ml) for 0, 2, or 4h; p38α serves as loading control. Data are representative of at least three independent experiments ( a-e ).

    Journal: Nature immunology

    Article Title: Interferon-γ regulates cellular metabolism and mRNA translation to potentiate macrophage activation

    doi: 10.1038/ni.3205

    Figure Lengend Snippet: IFN-γ suppresses mTORC1 activation and downstream functions ( a-c ) Immunoblot analysis of whole cell lysates from control or IFN-γ-treated macrophages stimulated with Pam3CSK4 (10 ng/ml) for the indicated times and probed with antibodies against p-4E-BP1 ( a , b ) or p-p70S6K ( c ). In ( b ), mTOR inhibitors PP242 (50 nM ), Torin1 (50 nM) or Rapamycin (500 nM) were added for 30 min. ( d ) Immunoblot analysis of LC3A and LC3B in control or IFN-γ-primed macrophages. ( e ) Upper: Immunofluorescence images of LAMP1 (red) and mTOR (green) co-staining in control or IFN-γ-primed macrophages stimulated with Pam3CSK4 (10 ng/ml) for 4 h; nuclei were stained with DAPI (blue). Quantitation of co-localization (lower panel) between LAMP1 and mTOR; data are presented as mean ± SEM of the percentage of co-localized cells from 600 cells analyzed in three independent experiments; * p = 0.0001 by unpaired student t test. ( f ) Immunoblot analysis of HES1 in human primary macrophages pretreated for 30min with vehicle control DMSO or increasing concentrations of Rapamycin (0 nM, 500 nM, 1 μM), and then stimulated with Pam3CSK4 (10 ng/ml) for 0, 2, or 4h; p38α serves as loading control. Data are representative of at least three independent experiments ( a-e ).

    Article Snippet: MG132, CGP57380, Rapamycin and PP242 were purchased from Calbiochem; Cycloheximide, Bafilomycin A1, 1-Methyl-D-tryptophan (1-D-MT), L-Tryptophan and 10058-F4 were from Sigma; Torin 1 was from R & D Systems; LY 294002 was from EMD Millipore.

    Techniques: Activation Assay, Immunofluorescence, Staining, Quantitation Assay