mnase digestion buffer  (Thermo Fisher)


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    Structured Review

    Thermo Fisher mnase digestion buffer
    Mnase Digestion Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mnase digestion buffer/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mnase digestion buffer - by Bioz Stars, 2021-01
    99/100 stars

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    Related Articles

    Overlay Assay:

    Article Title: Transgenic Overexpression of LARGE Induces ?-Dystroglycan Hyperglycosylation in Skeletal and Cardiac Muscle
    Article Snippet: .. For the laminin overlay assay, nitrocellulose membranes were blocked for 1 hour in laminin binding buffer (LBB: 10 mM triethanolamine, 140 mM NaCl, 1 mM MgCl2, 1 mM CaCl2, pH 7.6) containing 5% non-fat dry milk followed by incubation of mouse Engelbreth-Holm-Swarm laminin (Invitrogen,USA) overnight at 4°C in LBB. .. Membranes were washed and incubated with anti rabbit laminin (Sigma, USA) followed by HRP-anti rabbit IgG (Jackson ImmunoResearch, USA).

    Article Title: The transgenic expression of LARGE exacerbates the muscle phenotype of dystroglycanopathy mice
    Article Snippet: .. For the laminin overlay assay, nitrocellulose membranes were blocked for 1 hour in laminin binding buffer (LBB: 10 mM triethanolamine, 140 mM NaCl, 1 mM MgCl2 , 1 mM CaCl2 , pH 7.6) containing 5% non-fat dry milk followed by incubation of mouse Engelbreth-Holm-Swarm laminin (Invitrogen,USA) overnight at 4°C in LBB. .. Membranes were washed and incubated with anti rabbit laminin (Sigma, USA) followed by HRP-anti rabbit IgG (Jackson ImmunoResearch, USA).

    Article Title: Prenatal muscle development in a mouse model for the secondary dystroglycanopathies
    Article Snippet: .. For the laminin overlay assay, PVDF membranes were blocked for 1 h in laminin-binding buffer (LBB; 10 mM triethanolamine, 140 mM NaCl, 1 mM MgCl2, 1 mM CaCl2, pH 7.6) containing 5 % non-fat dry milk followed by incubation of 1 μg/ml mouse Engelbreth–Holm–Swarm (EHS) laminin protein (Invitrogen) in LBB overnight on a roller at 4 °C. .. The membranes were washed and incubated with rabbit anti-laminin (Sigma) followed by HRP-anti-rabbit IgG (Jackson ImmunoResearch).

    Concentration Assay:

    Article Title: Pumilio Homologue from Saprolegnia parasitica Specifically Expressed in Undifferentiated Spore Cysts
    Article Snippet: .. Each 12-μl PCR mixture contained cDNA corresponding to 25 ng of RNA, 1.5 mM MgCl2 , a 0.2 mM concentration of each deoxynucleoside triphosphate, a 0.4 mM concentration of each primer, and 1 U of Taq polymerase (Gibco) mixed with Platinum Antitaq antibodies (Gibco). ..

    Incubation:

    Article Title: Transgenic Overexpression of LARGE Induces ?-Dystroglycan Hyperglycosylation in Skeletal and Cardiac Muscle
    Article Snippet: .. For the laminin overlay assay, nitrocellulose membranes were blocked for 1 hour in laminin binding buffer (LBB: 10 mM triethanolamine, 140 mM NaCl, 1 mM MgCl2, 1 mM CaCl2, pH 7.6) containing 5% non-fat dry milk followed by incubation of mouse Engelbreth-Holm-Swarm laminin (Invitrogen,USA) overnight at 4°C in LBB. .. Membranes were washed and incubated with anti rabbit laminin (Sigma, USA) followed by HRP-anti rabbit IgG (Jackson ImmunoResearch, USA).

    Article Title: A unique insertion in STARD9's motor domain regulates its stability
    Article Snippet: .. After 3 h, a master mix containing all the ubiquitination components (ubiquitin [Enzo Life Sciences], ROC1, E1, E2, Skp1, with or without β-TrCP, with or without CUL1, in a buffer containing 20 mM HEPES, 5 mM NaCl, 5 mM MgCl2 , DTT, MG132, and protease and phosphatase inhibitor cocktail (Thermo Scientific) and ATP regeneration system was added to the tubes and further incubated for 90 min at 30°C. ..

    Article Title: Down-Regulation of Telomerase Activity and Activation of Caspase-3 Are Responsible for Tanshinone I-Induced Apoptosis in Monocyte Leukemia Cells in Vitro
    Article Snippet: .. To generate hTERT cDNA, a 20-μL reaction containing 1μg of total RNA, 25 mmol/L MgCl2 , 10 × PCR buffer, 10 mmol/LDntp, 0.1 MDTT, 1 unit/μL of RNase inhibitor, 200 units of murine leukemia virus reverse transcriptase (Life Technologies, Gaithersburg, Md., USA), and 2.5 μmol/L of random hexamers was incubated for 10 min at 65 °C. ..

    Article Title: The transgenic expression of LARGE exacerbates the muscle phenotype of dystroglycanopathy mice
    Article Snippet: .. For the laminin overlay assay, nitrocellulose membranes were blocked for 1 hour in laminin binding buffer (LBB: 10 mM triethanolamine, 140 mM NaCl, 1 mM MgCl2 , 1 mM CaCl2 , pH 7.6) containing 5% non-fat dry milk followed by incubation of mouse Engelbreth-Holm-Swarm laminin (Invitrogen,USA) overnight at 4°C in LBB. .. Membranes were washed and incubated with anti rabbit laminin (Sigma, USA) followed by HRP-anti rabbit IgG (Jackson ImmunoResearch, USA).

    Article Title: Prenatal muscle development in a mouse model for the secondary dystroglycanopathies
    Article Snippet: .. For the laminin overlay assay, PVDF membranes were blocked for 1 h in laminin-binding buffer (LBB; 10 mM triethanolamine, 140 mM NaCl, 1 mM MgCl2, 1 mM CaCl2, pH 7.6) containing 5 % non-fat dry milk followed by incubation of 1 μg/ml mouse Engelbreth–Holm–Swarm (EHS) laminin protein (Invitrogen) in LBB overnight on a roller at 4 °C. .. The membranes were washed and incubated with rabbit anti-laminin (Sigma) followed by HRP-anti-rabbit IgG (Jackson ImmunoResearch).

    Mass Spectrometry:

    Article Title: Selective ORAI1 inhibition ameliorates autoimmune CNS inflammation by suppressing effector but not regulatory T cell function
    Article Snippet: .. The MS result tables were exported and the concentrations of AMG1 were then calculated from a standard curve fitted by a weighted (1/x2 ) linear regression in Watson software (version 7.4; Thermo Fisher Scientific). ..

    Polymerase Chain Reaction:

    Article Title: Down-Regulation of Telomerase Activity and Activation of Caspase-3 Are Responsible for Tanshinone I-Induced Apoptosis in Monocyte Leukemia Cells in Vitro
    Article Snippet: .. To generate hTERT cDNA, a 20-μL reaction containing 1μg of total RNA, 25 mmol/L MgCl2 , 10 × PCR buffer, 10 mmol/LDntp, 0.1 MDTT, 1 unit/μL of RNase inhibitor, 200 units of murine leukemia virus reverse transcriptase (Life Technologies, Gaithersburg, Md., USA), and 2.5 μmol/L of random hexamers was incubated for 10 min at 65 °C. ..

    Article Title: Pumilio Homologue from Saprolegnia parasitica Specifically Expressed in Undifferentiated Spore Cysts
    Article Snippet: .. Each 12-μl PCR mixture contained cDNA corresponding to 25 ng of RNA, 1.5 mM MgCl2 , a 0.2 mM concentration of each deoxynucleoside triphosphate, a 0.4 mM concentration of each primer, and 1 U of Taq polymerase (Gibco) mixed with Platinum Antitaq antibodies (Gibco). ..

    Binding Assay:

    Article Title: Transgenic Overexpression of LARGE Induces ?-Dystroglycan Hyperglycosylation in Skeletal and Cardiac Muscle
    Article Snippet: .. For the laminin overlay assay, nitrocellulose membranes were blocked for 1 hour in laminin binding buffer (LBB: 10 mM triethanolamine, 140 mM NaCl, 1 mM MgCl2, 1 mM CaCl2, pH 7.6) containing 5% non-fat dry milk followed by incubation of mouse Engelbreth-Holm-Swarm laminin (Invitrogen,USA) overnight at 4°C in LBB. .. Membranes were washed and incubated with anti rabbit laminin (Sigma, USA) followed by HRP-anti rabbit IgG (Jackson ImmunoResearch, USA).

    Article Title: The transgenic expression of LARGE exacerbates the muscle phenotype of dystroglycanopathy mice
    Article Snippet: .. For the laminin overlay assay, nitrocellulose membranes were blocked for 1 hour in laminin binding buffer (LBB: 10 mM triethanolamine, 140 mM NaCl, 1 mM MgCl2 , 1 mM CaCl2 , pH 7.6) containing 5% non-fat dry milk followed by incubation of mouse Engelbreth-Holm-Swarm laminin (Invitrogen,USA) overnight at 4°C in LBB. .. Membranes were washed and incubated with anti rabbit laminin (Sigma, USA) followed by HRP-anti rabbit IgG (Jackson ImmunoResearch, USA).

    Software:

    Article Title: Selective ORAI1 inhibition ameliorates autoimmune CNS inflammation by suppressing effector but not regulatory T cell function
    Article Snippet: .. The MS result tables were exported and the concentrations of AMG1 were then calculated from a standard curve fitted by a weighted (1/x2 ) linear regression in Watson software (version 7.4; Thermo Fisher Scientific). ..

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  • 90
    Thermo Fisher mnase digestion buffer
    Mnase Digestion Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mnase digestion buffer/product/Thermo Fisher
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mnase digestion buffer - by Bioz Stars, 2021-01
    90/100 stars
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