mnase digestion buffer  (Thermo Fisher)


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    Structured Review

    Thermo Fisher mnase digestion buffer
    Mnase Digestion Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mnase digestion buffer/product/Thermo Fisher
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mnase digestion buffer - by Bioz Stars, 2020-08
    93/100 stars

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    Related Articles

    Methylation Sequencing:

    Article Title: Histone-Acetylated Control of Fibroblast Growth Factor Receptor 2 Intron 2 Polymorphisms and Isoform Splicing in Breast Cancer
    Article Snippet: .. Two rounds of PCRs were carried out for bisulfite sequencing, both for 10 min at 95 C followed by 40 cycles of 30 sec at 95 C, 45 sec at Tm and 45 sec at 72 C, followed by a 10-min extension at 72 C in a reaction mixture containing 1.5 m m MgCl2 , 0.2 m m dNTP, 0.2 m m of each primer, and 0.25 U/10 μl of Ampli Taq Gold polymerase (Applied Biosystems). .. Final PCR products were cut from 2.0% agarose gels, extracted, and cloned into the TA cloning system (Invitrogen) for automated sequencing.

    Synthesized:

    Article Title: Adrenomedullin2 (ADM2)/Intermedin (IMD) in Rat Ovary: Changes in Estrous Cycle and Pregnancy and Its Role in Ovulation and Steroidogenesis 1
    Article Snippet: .. Using total RNA, first-strand complementary DNA (cDNA) was synthesized by reverse transcription in a 20 μl reaction volume containing 1× PCR buffer, 2 μg RNA, 5 mM MgCl2 , 1 mM dNTP mixture, and random primers as described by the supplier (Ambion). ..

    Overlay Assay:

    Article Title: Transgenic Overexpression of LARGE Induces ?-Dystroglycan Hyperglycosylation in Skeletal and Cardiac Muscle
    Article Snippet: .. For the laminin overlay assay, nitrocellulose membranes were blocked for 1 hour in laminin binding buffer (LBB: 10 mM triethanolamine, 140 mM NaCl, 1 mM MgCl2, 1 mM CaCl2, pH 7.6) containing 5% non-fat dry milk followed by incubation of mouse Engelbreth-Holm-Swarm laminin (Invitrogen,USA) overnight at 4°C in LBB. .. Membranes were washed and incubated with anti rabbit laminin (Sigma, USA) followed by HRP-anti rabbit IgG (Jackson ImmunoResearch, USA).

    Article Title: The transgenic expression of LARGE exacerbates the muscle phenotype of dystroglycanopathy mice
    Article Snippet: .. For the laminin overlay assay, nitrocellulose membranes were blocked for 1 hour in laminin binding buffer (LBB: 10 mM triethanolamine, 140 mM NaCl, 1 mM MgCl2 , 1 mM CaCl2 , pH 7.6) containing 5% non-fat dry milk followed by incubation of mouse Engelbreth-Holm-Swarm laminin (Invitrogen,USA) overnight at 4°C in LBB. .. Membranes were washed and incubated with anti rabbit laminin (Sigma, USA) followed by HRP-anti rabbit IgG (Jackson ImmunoResearch, USA).

    Article Title: Prenatal muscle development in a mouse model for the secondary dystroglycanopathies
    Article Snippet: .. For the laminin overlay assay, PVDF membranes were blocked for 1 h in laminin-binding buffer (LBB; 10 mM triethanolamine, 140 mM NaCl, 1 mM MgCl2, 1 mM CaCl2, pH 7.6) containing 5 % non-fat dry milk followed by incubation of 1 μg/ml mouse Engelbreth–Holm–Swarm (EHS) laminin protein (Invitrogen) in LBB overnight on a roller at 4 °C. .. The membranes were washed and incubated with rabbit anti-laminin (Sigma) followed by HRP-anti-rabbit IgG (Jackson ImmunoResearch).

    Size-exclusion Chromatography:

    Article Title: Histone-Acetylated Control of Fibroblast Growth Factor Receptor 2 Intron 2 Polymorphisms and Isoform Splicing in Breast Cancer
    Article Snippet: .. Two rounds of PCRs were carried out for bisulfite sequencing, both for 10 min at 95 C followed by 40 cycles of 30 sec at 95 C, 45 sec at Tm and 45 sec at 72 C, followed by a 10-min extension at 72 C in a reaction mixture containing 1.5 m m MgCl2 , 0.2 m m dNTP, 0.2 m m of each primer, and 0.25 U/10 μl of Ampli Taq Gold polymerase (Applied Biosystems). .. Final PCR products were cut from 2.0% agarose gels, extracted, and cloned into the TA cloning system (Invitrogen) for automated sequencing.

    Concentration Assay:

    Article Title: Pumilio Homologue from Saprolegnia parasitica Specifically Expressed in Undifferentiated Spore Cysts
    Article Snippet: .. Each 12-μl PCR mixture contained cDNA corresponding to 25 ng of RNA, 1.5 mM MgCl2 , a 0.2 mM concentration of each deoxynucleoside triphosphate, a 0.4 mM concentration of each primer, and 1 U of Taq polymerase (Gibco) mixed with Platinum Antitaq antibodies (Gibco). ..

    Incubation:

    Article Title: Transgenic Overexpression of LARGE Induces ?-Dystroglycan Hyperglycosylation in Skeletal and Cardiac Muscle
    Article Snippet: .. For the laminin overlay assay, nitrocellulose membranes were blocked for 1 hour in laminin binding buffer (LBB: 10 mM triethanolamine, 140 mM NaCl, 1 mM MgCl2, 1 mM CaCl2, pH 7.6) containing 5% non-fat dry milk followed by incubation of mouse Engelbreth-Holm-Swarm laminin (Invitrogen,USA) overnight at 4°C in LBB. .. Membranes were washed and incubated with anti rabbit laminin (Sigma, USA) followed by HRP-anti rabbit IgG (Jackson ImmunoResearch, USA).

    Article Title: Down-Regulation of Telomerase Activity and Activation of Caspase-3 Are Responsible for Tanshinone I-Induced Apoptosis in Monocyte Leukemia Cells in Vitro
    Article Snippet: .. To generate hTERT cDNA, a 20-μL reaction containing 1μg of total RNA, 25 mmol/L MgCl2 , 10 × PCR buffer, 10 mmol/LDntp, 0.1 MDTT, 1 unit/μL of RNase inhibitor, 200 units of murine leukemia virus reverse transcriptase (Life Technologies, Gaithersburg, Md., USA), and 2.5 μmol/L of random hexamers was incubated for 10 min at 65 °C. ..

    Article Title: The transgenic expression of LARGE exacerbates the muscle phenotype of dystroglycanopathy mice
    Article Snippet: .. For the laminin overlay assay, nitrocellulose membranes were blocked for 1 hour in laminin binding buffer (LBB: 10 mM triethanolamine, 140 mM NaCl, 1 mM MgCl2 , 1 mM CaCl2 , pH 7.6) containing 5% non-fat dry milk followed by incubation of mouse Engelbreth-Holm-Swarm laminin (Invitrogen,USA) overnight at 4°C in LBB. .. Membranes were washed and incubated with anti rabbit laminin (Sigma, USA) followed by HRP-anti rabbit IgG (Jackson ImmunoResearch, USA).

    Article Title: Prenatal muscle development in a mouse model for the secondary dystroglycanopathies
    Article Snippet: .. For the laminin overlay assay, PVDF membranes were blocked for 1 h in laminin-binding buffer (LBB; 10 mM triethanolamine, 140 mM NaCl, 1 mM MgCl2, 1 mM CaCl2, pH 7.6) containing 5 % non-fat dry milk followed by incubation of 1 μg/ml mouse Engelbreth–Holm–Swarm (EHS) laminin protein (Invitrogen) in LBB overnight on a roller at 4 °C. .. The membranes were washed and incubated with rabbit anti-laminin (Sigma) followed by HRP-anti-rabbit IgG (Jackson ImmunoResearch).

    Polymerase Chain Reaction:

    Article Title: Adrenomedullin2 (ADM2)/Intermedin (IMD) in Rat Ovary: Changes in Estrous Cycle and Pregnancy and Its Role in Ovulation and Steroidogenesis 1
    Article Snippet: .. Using total RNA, first-strand complementary DNA (cDNA) was synthesized by reverse transcription in a 20 μl reaction volume containing 1× PCR buffer, 2 μg RNA, 5 mM MgCl2 , 1 mM dNTP mixture, and random primers as described by the supplier (Ambion). ..

    Article Title: Down-Regulation of Telomerase Activity and Activation of Caspase-3 Are Responsible for Tanshinone I-Induced Apoptosis in Monocyte Leukemia Cells in Vitro
    Article Snippet: .. To generate hTERT cDNA, a 20-μL reaction containing 1μg of total RNA, 25 mmol/L MgCl2 , 10 × PCR buffer, 10 mmol/LDntp, 0.1 MDTT, 1 unit/μL of RNase inhibitor, 200 units of murine leukemia virus reverse transcriptase (Life Technologies, Gaithersburg, Md., USA), and 2.5 μmol/L of random hexamers was incubated for 10 min at 65 °C. ..

    Article Title: Pumilio Homologue from Saprolegnia parasitica Specifically Expressed in Undifferentiated Spore Cysts
    Article Snippet: .. Each 12-μl PCR mixture contained cDNA corresponding to 25 ng of RNA, 1.5 mM MgCl2 , a 0.2 mM concentration of each deoxynucleoside triphosphate, a 0.4 mM concentration of each primer, and 1 U of Taq polymerase (Gibco) mixed with Platinum Antitaq antibodies (Gibco). ..

    Binding Assay:

    Article Title: Transgenic Overexpression of LARGE Induces ?-Dystroglycan Hyperglycosylation in Skeletal and Cardiac Muscle
    Article Snippet: .. For the laminin overlay assay, nitrocellulose membranes were blocked for 1 hour in laminin binding buffer (LBB: 10 mM triethanolamine, 140 mM NaCl, 1 mM MgCl2, 1 mM CaCl2, pH 7.6) containing 5% non-fat dry milk followed by incubation of mouse Engelbreth-Holm-Swarm laminin (Invitrogen,USA) overnight at 4°C in LBB. .. Membranes were washed and incubated with anti rabbit laminin (Sigma, USA) followed by HRP-anti rabbit IgG (Jackson ImmunoResearch, USA).

    Article Title: The transgenic expression of LARGE exacerbates the muscle phenotype of dystroglycanopathy mice
    Article Snippet: .. For the laminin overlay assay, nitrocellulose membranes were blocked for 1 hour in laminin binding buffer (LBB: 10 mM triethanolamine, 140 mM NaCl, 1 mM MgCl2 , 1 mM CaCl2 , pH 7.6) containing 5% non-fat dry milk followed by incubation of mouse Engelbreth-Holm-Swarm laminin (Invitrogen,USA) overnight at 4°C in LBB. .. Membranes were washed and incubated with anti rabbit laminin (Sigma, USA) followed by HRP-anti rabbit IgG (Jackson ImmunoResearch, USA).

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  • News
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  • Team
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  • Bioz Stars
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  • 91
    Thermo Fisher mnase digestion buffer
    Mnase Digestion Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mnase digestion buffer/product/Thermo Fisher
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mnase digestion buffer - by Bioz Stars, 2020-08
    91/100 stars
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