Journal: Mediators of Inflammation

Article Title: Study of the Correlations among Some Parameters of the Oxidative Status, Gelatinases, and Their Inhibitors in a Group of Subjects with Metabolic Syndrome

doi: 10.1155/2014/510619

Figure Lengend Snippet: Medians (interquartile ranges) of gelatinase and inhibitor plasma concentrations in normal controls, in the whole group of MS patients, and in the two subgroups, respectively, with and without diabetes mellitus.

Article Snippet: Plasma concentrations of gelatinases (MMP-2 and MMP-9) and their inhibitors (TIMP-1 and TIMP-2) were determined using, respectively, the Human MMP-2 ELISA and Human MMP-9 ELISA kit (Boster Biological Technology, Ltd.) and the Human TIMP-1 ELISA and Human TIMP-2 ELISA kit (Boster Biological Technology, Ltd.).

Techniques:

Journal: Mediators of Inflammation

Article Title: Study of the Correlations among Some Parameters of the Oxidative Status, Gelatinases, and Their Inhibitors in a Group of Subjects with Metabolic Syndrome

doi: 10.1155/2014/510619

Figure Lengend Snippet: Correlations between oxidative parameters and gelatinases in diabetic MS subjects.

Article Snippet: Plasma concentrations of gelatinases (MMP-2 and MMP-9) and their inhibitors (TIMP-1 and TIMP-2) were determined using, respectively, the Human MMP-2 ELISA and Human MMP-9 ELISA kit (Boster Biological Technology, Ltd.) and the Human TIMP-1 ELISA and Human TIMP-2 ELISA kit (Boster Biological Technology, Ltd.).

Techniques:

Journal: Mediators of Inflammation

Article Title: Study of the Correlations among Some Parameters of the Oxidative Status, Gelatinases, and Their Inhibitors in a Group of Subjects with Metabolic Syndrome

doi: 10.1155/2014/510619

Figure Lengend Snippet: Correlations between oxidative parameters and gelatinases in all MS subjects.

Article Snippet: Plasma concentrations of gelatinases (MMP-2 and MMP-9) and their inhibitors (TIMP-1 and TIMP-2) were determined using, respectively, the Human MMP-2 ELISA and Human MMP-9 ELISA kit (Boster Biological Technology, Ltd.) and the Human TIMP-1 ELISA and Human TIMP-2 ELISA kit (Boster Biological Technology, Ltd.).

Techniques:

Journal: Mediators of Inflammation

Article Title: Study of the Correlations among Some Parameters of the Oxidative Status, Gelatinases, and Their Inhibitors in a Group of Subjects with Metabolic Syndrome

doi: 10.1155/2014/510619

Figure Lengend Snippet: Correlations between oxidative parameters and gelatinases in nondiabetic MS subjects.

Article Snippet: Plasma concentrations of gelatinases (MMP-2 and MMP-9) and their inhibitors (TIMP-1 and TIMP-2) were determined using, respectively, the Human MMP-2 ELISA and Human MMP-9 ELISA kit (Boster Biological Technology, Ltd.) and the Human TIMP-1 ELISA and Human TIMP-2 ELISA kit (Boster Biological Technology, Ltd.).

Techniques:

Journal: Journal of Immunology Research

Article Title: Effect of SIS3 on Extracellular Matrix Remodeling and Repair in a Lipopolysaccharide-Induced ARDS Rat Model

doi: 10.1155/2020/6644687

Figure Lengend Snippet: The rat primer sequences used in this study.

Article Snippet: RAGE, TGF- β 1, MMP2, and MMP9 concentrations in the rat sera and BALF were determined using a rat RAGE ELISA kit (EK0971 from Boster Biological Technology, China), a rat TGF- β 1 ELISA kit (MB100B, R&D Systems), a rat MMP2 ELISA kit (MMP200, R&D Systems), and a rat MMP9 ELISA kit (CSB-E08008r Cusabio, Wuhan, China), respectively, following the manufacturer's protocols.

Techniques:

Journal: Journal of Immunology Research

Article Title: Effect of SIS3 on Extracellular Matrix Remodeling and Repair in a Lipopolysaccharide-Induced ARDS Rat Model

doi: 10.1155/2020/6644687

Figure Lengend Snippet: SIS3 inhibits mRNA expression of RAGE, TGF- β 1, MMP2, and MMP9 in lung homogenates of ARDS rats. The mRNA levels of RAGE, TGF- β 1, MMP2, and MMP9 were determined using real-time PCR and were standardized to β -actin. The results suggested that SIS3 inhibited the increase of (a) RAGE, (b) TGF- β 1, (c) MMP2, and (d) MMP9 in LPS-induced lung homogenates of ARDS rats, while no effect was seen upon pretreatment with PBS. The data are presented as the means ± SD ( n = 10). ★ P < 0.05 vs. CTL; # P < 0.05 vs. ARDS.

Article Snippet: RAGE, TGF- β 1, MMP2, and MMP9 concentrations in the rat sera and BALF were determined using a rat RAGE ELISA kit (EK0971 from Boster Biological Technology, China), a rat TGF- β 1 ELISA kit (MB100B, R&D Systems), a rat MMP2 ELISA kit (MMP200, R&D Systems), and a rat MMP9 ELISA kit (CSB-E08008r Cusabio, Wuhan, China), respectively, following the manufacturer's protocols.

Techniques: Expressing, Real-time Polymerase Chain Reaction

Journal: Journal of Immunology Research

Article Title: Effect of SIS3 on Extracellular Matrix Remodeling and Repair in a Lipopolysaccharide-Induced ARDS Rat Model

doi: 10.1155/2020/6644687

Figure Lengend Snippet: SIS3 pretreatment decreases the protein expression and localization of RAGE, TGF- β 1, MMP2, and MMP9 in ARDS rats. Immunohistochemical staining of lung tissue sections showed that RAGE, TGF- β 1, MMP2, and MMP9 were expressed in the bronchial smooth muscle, airways, and alveolar epithelial cells of rats. The expression levels of RAGE, TGF- β 1, MMP2, and MMP9 in the ECM of ARDS rats induced by LPS were significantly higher than those of the control group. RAGE, TGF- β 1, MMP2, and MMP9 were reduced by pretreatment with SIS3, and no effect was found upon pretreatment with PBS. The micrographs were magnified at 400x. The red triangles indicate infiltrated leukocytes.

Article Snippet: RAGE, TGF- β 1, MMP2, and MMP9 concentrations in the rat sera and BALF were determined using a rat RAGE ELISA kit (EK0971 from Boster Biological Technology, China), a rat TGF- β 1 ELISA kit (MB100B, R&D Systems), a rat MMP2 ELISA kit (MMP200, R&D Systems), and a rat MMP9 ELISA kit (CSB-E08008r Cusabio, Wuhan, China), respectively, following the manufacturer's protocols.

Techniques: Expressing, Immunohistochemical staining, Staining

Journal: Journal of Immunology Research

Article Title: Effect of SIS3 on Extracellular Matrix Remodeling and Repair in a Lipopolysaccharide-Induced ARDS Rat Model

doi: 10.1155/2020/6644687

Figure Lengend Snippet: SIS3 inhibited the protein expression of RAGE, TGF- β 1, MMP2, and MMP9 proteins in lung homogenates acquired from ARDS rats. The expression levels of RAGE, TGF- β 1, MMP2, and MMP9 proteins in lung tissue homogenates, sera, and BALF of ARDS rats and were determined using western blotting analysis at 24 h after LPS intervention. The results of western blotting showed that SIS3 inhibited LPS-induced (a) RAGE, (b) TGF- β 1, (c) MMP2, and (d) and MMP9 protein expression in the lung homogenates. The data are presented as the means ± SD ( n = 10). ★ P < 0.05 vs. CTL; # P < 0.05 vs. ARDS.

Article Snippet: RAGE, TGF- β 1, MMP2, and MMP9 concentrations in the rat sera and BALF were determined using a rat RAGE ELISA kit (EK0971 from Boster Biological Technology, China), a rat TGF- β 1 ELISA kit (MB100B, R&D Systems), a rat MMP2 ELISA kit (MMP200, R&D Systems), and a rat MMP9 ELISA kit (CSB-E08008r Cusabio, Wuhan, China), respectively, following the manufacturer's protocols.

Techniques: Expressing, Western Blot

Journal: Journal of Immunology Research

Article Title: Effect of SIS3 on Extracellular Matrix Remodeling and Repair in a Lipopolysaccharide-Induced ARDS Rat Model

doi: 10.1155/2020/6644687

Figure Lengend Snippet: SIS3 pretreatment prevented the reduction of the expression of RAGE, TGF- β 1, MMP2, and MMP9 in LPS-induced ARDS. The effects of SIS3 on the protein expression levels of (a) RAGE, (b) TGF- β 1, (c) MMP2, and (d) MMP9 in the BALF and sera of the ARDS rats were determined using ELISA. The results demonstrated that the levels of RAGE, TGF- β 1, MMP2, and MMP9 proteins were significantly higher in the ARDS group than in the control group, while the levels of RAGE, TGF- β 1, MMP2, and MMP9 in the SIS3 group were lower than those in the ARDS group. The protein levels of RAGE, TGF- β 1, MMP2, and MMP9 in LPS-administered pretreatment with PBS were not different from those of the ARDS group. The data are presented as the means ± SD of three independent experiments in triplicate ( n = 10). ★ P < 0.05 vs. CTL; # P < 0.05 vs. ARDS.

Article Snippet: RAGE, TGF- β 1, MMP2, and MMP9 concentrations in the rat sera and BALF were determined using a rat RAGE ELISA kit (EK0971 from Boster Biological Technology, China), a rat TGF- β 1 ELISA kit (MB100B, R&D Systems), a rat MMP2 ELISA kit (MMP200, R&D Systems), and a rat MMP9 ELISA kit (CSB-E08008r Cusabio, Wuhan, China), respectively, following the manufacturer's protocols.

Techniques: Expressing, Enzyme-linked Immunosorbent Assay

Journal: Experimental and Therapeutic Medicine

Article Title: FOXP3 facilitates the invasion and metastasis of non-small cell lung cancer cells through regulating VEGF, EMT and the Notch1/Hes1 pathway

doi: 10.3892/etm.2021.10390

Figure Lengend Snippet: FOXP3 promotes the migratory and invasive abilities of A549 cells. (A) Reverse transcription quantitative PCR assessing the efficacy of FOXP3 knockdown using specific siRNAs in A549 cells. (B) Western blotting assessing the efficacy of FOXP3 knockdown using specific siRNAs in A549 cells. (C) Transwell assays assessing the cell migratory and invasive abilities of A549 following FOXP3 knockdown. (D and E) ELISA detection of MMP-2 and MMP-9 secretion from A549 cells 72 h following FOXP3 knockdown. ** P<0.01. FOXP3, forkhead box P3; MMP-2, matrix metalloproteinase-2; MMP-9, matrix metalloproteinase-9; si, small interfering; NC, negative control.

Article Snippet: The concentrations of matrix metalloproteinase-2 (MMP-2), MMP-9 and VEGF in the culture supernatant were detected using ELISA kits (cat. no. EK0459; cat. no. EK0465; cat. no. EK0539; Boster Biological Technology) according to the manufacturers' protocol.

Techniques: Real-time Polymerase Chain Reaction, Western Blot, Enzyme-linked Immunosorbent Assay, Negative Control

Journal: Experimental and Therapeutic Medicine

Article Title: FOXP3 facilitates the invasion and metastasis of non-small cell lung cancer cells through regulating VEGF, EMT and the Notch1/Hes1 pathway

doi: 10.3892/etm.2021.10390

Figure Lengend Snippet: Expression of FOXP3 and CD31 in NSCLC tissues and regulation of VEGF expression by FOXP3 in A549 cells. (A) FOXP3 expression was positively associated with CD31 + vascular endothelial cells in individual NSCLC tissue samples according to immunohistochemistry staining (magnification, x400). a and c show positive expression of FOXP3 and CD31; b and d show negative expression of FOXP3 and CD31. (B) Expression levels of VEGF were detected by reverse transcription quantitative PCR 48 h following FOXP3 knockdown in A549 cells. (C) Expression levels of VEGF were detected by ELISA assay 72 h following FOXP3 knockdown in A549 cells. ** P<0.01. FOXP3, forkhead box P3; VEGF, vascular endothelial growth factor; NSCLC, non-small cell lung cancer; si, small interfering; NC, negative control.

Article Snippet: The concentrations of matrix metalloproteinase-2 (MMP-2), MMP-9 and VEGF in the culture supernatant were detected using ELISA kits (cat. no. EK0459; cat. no. EK0465; cat. no. EK0539; Boster Biological Technology) according to the manufacturers' protocol.

Techniques: Expressing, Immunohistochemistry, Staining, Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay, Negative Control

Journal: International Journal of Molecular Sciences

Article Title: HGF and TSG-6 Released by Mesenchymal Stem Cells Attenuate Colon Radiation-Induced Fibrosis

doi: 10.3390/ijms22041790

Figure Lengend Snippet: Effect of MSC transplantation on radiation-induced ECM remodeling. ( A ) Timeline of irradiation and MSC transplantation protocol. ( B ) mRNA fold changes of genes 4 weeks after irradiation (red dots) and 1 week after MSC injection (blue dots) that are normalized to Ywhaz and standardized against Control group. mRNA fold changes of genes encoding CTGF and ECM components indicate suppression of pro-fibrotic signals by MSCs. ( C ) Five weeks after irradiation: MSCs increase secretion of MMP-2 in the irradiated colon–rectum area. ( D ) Percentage of fibrosis area quantified on Picrosirius Red-stained slides. MSCs significantly inhibit ECM deposition in the colon–rectum area of irradiated animals as observed 7 weeks after irradiation. ( E ) Representative images showing ECM (black arrow) stained with Picrosirius red (original magnification: 40×) in the colon–rectum area of irradiated + MSCs group, 7 weeks after irradiation (original magnification: 40×). Each group of animals was composed of 6 rats. The results of each group were compared as follows: Irradiated group was compared to Control group; Irradiated + MSCs group was compared to control and Irradiated group. Two-group comparisons were performed with t -tests, while one-way ANOVAs, followed by Bonferroni t -tests, were used for multiple group comparisons. The results are expressed as mean ± SD. *: p < 0.05; **: p < 0.01; ***: p ≤ 0.001.

Article Snippet: ELISAs for rat MMP-2 (ref: EK0639, Boster Biological Technology Co., Ltd., Pleasanton, CA, USA), MMP-9 (ref: E-EL-R0624, Elabscience Co., Ltd., Wuhan Shi, China) and TGF-β1 (ref: EK0514, Boster Biological Technology Co., Ltd.) were performed as instructed by their respective manufacturers.

Techniques: Transplantation Assay, Irradiation, Injection, Staining

Journal: Aging (Albany NY)

Article Title: The effect of swimming exercise and diet on the hypothalamic inflammation of ApoE-/- mice based on SIRT1-NF-κB-GnRH expression

doi: 10.18632/aging.103323

Figure Lengend Snippet: Serum IL-1β, TNFα, MMP-2, MMP-9 and SIRT1 expression. ( A ) The levels of IL-1β in serum. ( B ) The levels of TNFα in serum. ( C ) The levels of MMP-2 in serum. ( D ) The levels of MMP-9 in serum. vs control group, ( E ) The levels of SIRT1 in serum. ▲▲ p < 0.01. vs HFD group, * p < 0.05, ** p < 0.01. vs DC group, # p < 0.05.

Article Snippet: ELISA Kits of mouse MMP-2, MMP-9, TNF-α and IL-1β (The catalog numbers: EK0460, EK0466, EK0527, EK0394) were purchased from BOSTER Biological Technology.co.ltd (Wuhan, China).

Techniques: Expressing

Journal: Aging (Albany NY)

Article Title: The effect of swimming exercise and diet on the hypothalamic inflammation of ApoE-/- mice based on SIRT1-NF-κB-GnRH expression

doi: 10.18632/aging.103323

Figure Lengend Snippet: The expression of SIRT1, NF-κB, TNFα, and IL-1β proteins in the hypothalamus. ( A ) Western blot to detect the expression of SIRT1, NF-κB p65, TNF-α and IL-1β of the hypothalamus. ( B ) The expression of SIRT1 in the hypothalamus. ( C ) The expression of NF-κB p65 in the hypothalamus. ( D ) The expression of TNF-α in the hypothalamus. ( E ) The expression of IL-1β in the hypothalamus. vs control group, ▲▲ p < 0.01. vs HFD group, * p < 0.05, ** p < 0.01. vs DC group, # p < 0.05, ## p < 0.01.

Article Snippet: ELISA Kits of mouse MMP-2, MMP-9, TNF-α and IL-1β (The catalog numbers: EK0460, EK0466, EK0527, EK0394) were purchased from BOSTER Biological Technology.co.ltd (Wuhan, China).

Techniques: Expressing, Western Blot

Journal: The Journal of International Medical Research

Article Title: Levels of matrix metalloproteinase-2 in committed differentiation of bone marrow mesenchymal stem cells induced by kartogenin

doi: 10.1177/0300060519853399

Figure Lengend Snippet: Matrix metalloproteinase-2 (MMP-2) concentrations in the culture supernatant of the different human mesenchymal stem cell treatment groups as detected by an enzyme-linked immunosorbent assay and presented as optical intensity (A) values. The results showed that the concentration of MMP-2 in the cell culture supernatant increased after induction with 1 µM kartogenin (KGN) compared with the control group ( P < 0.05), but the MMP-2 concentration in the culture supernatant decreased significantly when the KGN concentration was increased to 10 µM compared with the 1 µM KGN group ( P < 0.05); Kruskal–Wallis test. The colour version of this figure is available at: http://imr.sagepub.com .

Article Snippet: An enzyme-linked immunosorbent assay (ELISA) was used to determine the concentration of MMP-2 in the cell culture supernatant according to the manufacturer’s instructions (human total MMP-2 ELISA Kit; Boster Biological Technology, Pleasanton, CA, USA) using an ELISA microplate reader (Beijing Perlong New Technology, Beijing, China).

Techniques: Enzyme-linked Immunosorbent Assay, Concentration Assay, Cell Culture

Journal: The Journal of International Medical Research

Article Title: Levels of matrix metalloproteinase-2 in committed differentiation of bone marrow mesenchymal stem cells induced by kartogenin

doi: 10.1177/0300060519853399

Figure Lengend Snippet: Matrix metalloproteinase-2 (MMP-2) concentrations in the culture supernatant of the different treatment groups as detected by an enzyme-linked immunosorbent assay.

Article Snippet: An enzyme-linked immunosorbent assay (ELISA) was used to determine the concentration of MMP-2 in the cell culture supernatant according to the manufacturer’s instructions (human total MMP-2 ELISA Kit; Boster Biological Technology, Pleasanton, CA, USA) using an ELISA microplate reader (Beijing Perlong New Technology, Beijing, China).

Techniques: Concentration Assay