mmp 9  (Boster Bio)


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    Boster Bio mmp 9
    FOXP3 promotes the migratory and invasive abilities of A549 cells. (A) Reverse transcription quantitative PCR assessing the efficacy of FOXP3 knockdown using specific siRNAs in A549 cells. (B) Western blotting assessing the efficacy of FOXP3 knockdown using specific siRNAs in A549 cells. (C) Transwell assays assessing the cell migratory and invasive abilities of A549 following FOXP3 knockdown. (D and E) ELISA detection of MMP-2 and <t>MMP-9</t> secretion from A549 cells 72 h following FOXP3 knockdown. ** P
    Mmp 9, supplied by Boster Bio, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    mmp 9 - by Bioz Stars, 2022-07
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    Images

    1) Product Images from "FOXP3 facilitates the invasion and metastasis of non-small cell lung cancer cells through regulating VEGF, EMT and the Notch1/Hes1 pathway"

    Article Title: FOXP3 facilitates the invasion and metastasis of non-small cell lung cancer cells through regulating VEGF, EMT and the Notch1/Hes1 pathway

    Journal: Experimental and Therapeutic Medicine

    doi: 10.3892/etm.2021.10390

    FOXP3 promotes the migratory and invasive abilities of A549 cells. (A) Reverse transcription quantitative PCR assessing the efficacy of FOXP3 knockdown using specific siRNAs in A549 cells. (B) Western blotting assessing the efficacy of FOXP3 knockdown using specific siRNAs in A549 cells. (C) Transwell assays assessing the cell migratory and invasive abilities of A549 following FOXP3 knockdown. (D and E) ELISA detection of MMP-2 and MMP-9 secretion from A549 cells 72 h following FOXP3 knockdown. ** P
    Figure Legend Snippet: FOXP3 promotes the migratory and invasive abilities of A549 cells. (A) Reverse transcription quantitative PCR assessing the efficacy of FOXP3 knockdown using specific siRNAs in A549 cells. (B) Western blotting assessing the efficacy of FOXP3 knockdown using specific siRNAs in A549 cells. (C) Transwell assays assessing the cell migratory and invasive abilities of A549 following FOXP3 knockdown. (D and E) ELISA detection of MMP-2 and MMP-9 secretion from A549 cells 72 h following FOXP3 knockdown. ** P

    Techniques Used: Real-time Polymerase Chain Reaction, Western Blot, Enzyme-linked Immunosorbent Assay

    2) Product Images from "Inhibition of acute lung injury by rubriflordilactone in LPS-induced rat model through suppression of inflammatory factor expression"

    Article Title: Inhibition of acute lung injury by rubriflordilactone in LPS-induced rat model through suppression of inflammatory factor expression

    Journal: International Journal of Clinical and Experimental Pathology

    doi:

    Inhibition of factors involved in inflammatory processes by rubriflordilactone treatment in LPS administered MLE-15 cells. In MLE-15 cells LPS exposure increased expression of MMP-9, however, rubriflordilactone treated suppressed the effect of LPS onMMP-9 expression. Rubriflordilactone inhibited the LPS induced increase in the expression of mRNA corresponding to iNOS, MMP-9 and IL-6. Rubriflordilactone inhibited the LPS induced reduction in the expression of Sirt1 in MLE-15 cells. Rub, LPS, Sirt1, MMP-9, IL and iNOS stands for rubriflordilactone, lipopolysaccharide, sirtuin 1, matrix metalloproteinase-9, interleukin and inducible nitric oxide synthase.
    Figure Legend Snippet: Inhibition of factors involved in inflammatory processes by rubriflordilactone treatment in LPS administered MLE-15 cells. In MLE-15 cells LPS exposure increased expression of MMP-9, however, rubriflordilactone treated suppressed the effect of LPS onMMP-9 expression. Rubriflordilactone inhibited the LPS induced increase in the expression of mRNA corresponding to iNOS, MMP-9 and IL-6. Rubriflordilactone inhibited the LPS induced reduction in the expression of Sirt1 in MLE-15 cells. Rub, LPS, Sirt1, MMP-9, IL and iNOS stands for rubriflordilactone, lipopolysaccharide, sirtuin 1, matrix metalloproteinase-9, interleukin and inducible nitric oxide synthase.

    Techniques Used: Inhibition, Expressing

    Effect of silencer RNA for Sirt1 on inhibition of acute lung injury by rubriflordilactone inMLE-15 cells. The cells transfected with siRNA for Sirt1 were exposed to LPS after rubriflordilactone treatment and then analyzed by western blot analysis. Rub, LPS, Sirt1, MMP-9, IL and iNOS stand for rubriflordilactone, lipopolysaccharide, sirtuin 1, matrix metalloproteinase-9, interleukin and inducible nitric oxide synthase.
    Figure Legend Snippet: Effect of silencer RNA for Sirt1 on inhibition of acute lung injury by rubriflordilactone inMLE-15 cells. The cells transfected with siRNA for Sirt1 were exposed to LPS after rubriflordilactone treatment and then analyzed by western blot analysis. Rub, LPS, Sirt1, MMP-9, IL and iNOS stand for rubriflordilactone, lipopolysaccharide, sirtuin 1, matrix metalloproteinase-9, interleukin and inducible nitric oxide synthase.

    Techniques Used: Inhibition, Transfection, Western Blot

    3) Product Images from "Inhibition of acute lung injury by rubriflordilactone in LPS-induced rat model through suppression of inflammatory factor expression"

    Article Title: Inhibition of acute lung injury by rubriflordilactone in LPS-induced rat model through suppression of inflammatory factor expression

    Journal: International Journal of Clinical and Experimental Pathology

    doi:

    Inhibition of factors involved in inflammatory processes by rubriflordilactone treatment in LPS administered MLE-15 cells. In MLE-15 cells LPS exposure increased expression of MMP-9, however, rubriflordilactone treated suppressed the effect of LPS onMMP-9 expression. Rubriflordilactone inhibited the LPS induced increase in the expression of mRNA corresponding to iNOS, MMP-9 and IL-6. Rubriflordilactone inhibited the LPS induced reduction in the expression of Sirt1 in MLE-15 cells. Rub, LPS, Sirt1, MMP-9, IL and iNOS stands for rubriflordilactone, lipopolysaccharide, sirtuin 1, matrix metalloproteinase-9, interleukin and inducible nitric oxide synthase.
    Figure Legend Snippet: Inhibition of factors involved in inflammatory processes by rubriflordilactone treatment in LPS administered MLE-15 cells. In MLE-15 cells LPS exposure increased expression of MMP-9, however, rubriflordilactone treated suppressed the effect of LPS onMMP-9 expression. Rubriflordilactone inhibited the LPS induced increase in the expression of mRNA corresponding to iNOS, MMP-9 and IL-6. Rubriflordilactone inhibited the LPS induced reduction in the expression of Sirt1 in MLE-15 cells. Rub, LPS, Sirt1, MMP-9, IL and iNOS stands for rubriflordilactone, lipopolysaccharide, sirtuin 1, matrix metalloproteinase-9, interleukin and inducible nitric oxide synthase.

    Techniques Used: Inhibition, Expressing

    Effect of silencer RNA for Sirt1 on inhibition of acute lung injury by rubriflordilactone inMLE-15 cells. The cells transfected with siRNA for Sirt1 were exposed to LPS after rubriflordilactone treatment and then analyzed by western blot analysis. Rub, LPS, Sirt1, MMP-9, IL and iNOS stand for rubriflordilactone, lipopolysaccharide, sirtuin 1, matrix metalloproteinase-9, interleukin and inducible nitric oxide synthase.
    Figure Legend Snippet: Effect of silencer RNA for Sirt1 on inhibition of acute lung injury by rubriflordilactone inMLE-15 cells. The cells transfected with siRNA for Sirt1 were exposed to LPS after rubriflordilactone treatment and then analyzed by western blot analysis. Rub, LPS, Sirt1, MMP-9, IL and iNOS stand for rubriflordilactone, lipopolysaccharide, sirtuin 1, matrix metalloproteinase-9, interleukin and inducible nitric oxide synthase.

    Techniques Used: Inhibition, Transfection, Western Blot

    4) Product Images from "Expression levels of matrix metalloproteinase-9 in human gastric carcinoma"

    Article Title: Expression levels of matrix metalloproteinase-9 in human gastric carcinoma

    Journal: Oncology Letters

    doi: 10.3892/ol.2014.2768

    mRNA expression of MMP-9 and β-actin extracted from gastric carcinoma tissue (ca), distal gastric mucosa tissue (n1) and healthy gastric tissue (n2). MMP-9, matrix metalloproteinase-9.
    Figure Legend Snippet: mRNA expression of MMP-9 and β-actin extracted from gastric carcinoma tissue (ca), distal gastric mucosa tissue (n1) and healthy gastric tissue (n2). MMP-9, matrix metalloproteinase-9.

    Techniques Used: Expressing

    5) Product Images from "Peptoanaerobacter stomatis Primes Human Neutrophils and Induces Granule Exocytosis"

    Article Title: Peptoanaerobacter stomatis Primes Human Neutrophils and Induces Granule Exocytosis

    Journal: Infection and Immunity

    doi: 10.1128/IAI.01043-16

    (A) P. stomatis induces the exocytosis of secretory vesicles and gelatinase granules. Neutrophils were unchallenged (basal) or challenged with fMLF (5 min), with P. stomatis (30 min), or with P. gingivalis (30 min). (A and B) Secretory vesicle exocytosis was determined by the increase in plasma membrane expression of CD35 by flow cytometry. Data are expressed as the means ± SEM of the mean channel of fluorescence (mcf) from 3 independent experiments. (C) Gelatinase granule exocytosis was measured as levels of matrix metalloproteinase 9 (MMP-9) present in the supernatants collected from the different experimental conditions by ELISA. Data are expressed as means ± SEM of MMP-9 release (in nanograms per 4 × 10 6 cells) from 4 independent experiments. (D) The activities of MMP-9 on the supernatants from the same experimental conditions were analyzed by gelatin zymogen assays. The supernatant from P. stomatis growth media (GS) was also analyzed. The protein standard is shown on the left. MMP-9 isoforms are indicated. (E and F) The densitometry profile of the degraded gelatin zymography 92-kDa band was analyzed by ImageJ software from the gels of 4 independent experiments (E) or from 3 independent experiments (F). *, P
    Figure Legend Snippet: (A) P. stomatis induces the exocytosis of secretory vesicles and gelatinase granules. Neutrophils were unchallenged (basal) or challenged with fMLF (5 min), with P. stomatis (30 min), or with P. gingivalis (30 min). (A and B) Secretory vesicle exocytosis was determined by the increase in plasma membrane expression of CD35 by flow cytometry. Data are expressed as the means ± SEM of the mean channel of fluorescence (mcf) from 3 independent experiments. (C) Gelatinase granule exocytosis was measured as levels of matrix metalloproteinase 9 (MMP-9) present in the supernatants collected from the different experimental conditions by ELISA. Data are expressed as means ± SEM of MMP-9 release (in nanograms per 4 × 10 6 cells) from 4 independent experiments. (D) The activities of MMP-9 on the supernatants from the same experimental conditions were analyzed by gelatin zymogen assays. The supernatant from P. stomatis growth media (GS) was also analyzed. The protein standard is shown on the left. MMP-9 isoforms are indicated. (E and F) The densitometry profile of the degraded gelatin zymography 92-kDa band was analyzed by ImageJ software from the gels of 4 independent experiments (E) or from 3 independent experiments (F). *, P

    Techniques Used: Expressing, Flow Cytometry, Cytometry, Fluorescence, Enzyme-linked Immunosorbent Assay, Zymography, Software

    6) Product Images from "Orcinol Glucoside Improves Senile Osteoporosis through Attenuating Oxidative Stress and Autophagy of Osteoclast via Activating Nrf2/Keap1 and mTOR Signaling Pathway"

    Article Title: Orcinol Glucoside Improves Senile Osteoporosis through Attenuating Oxidative Stress and Autophagy of Osteoclast via Activating Nrf2/Keap1 and mTOR Signaling Pathway

    Journal: Oxidative Medicine and Cellular Longevity

    doi: 10.1155/2022/5410377

    OG suppresses bone resorption in SAMP6 mice and osteoclast derived from RAW264.7 cells. (a) Representative Western blot analysis and the quantification of NFATc1, c-FOS, CTSK, and MMP9 expression in bone tissue of SAMP6 mice. (b) Osteoclasts were labeled with phalloidin (F-actin ring) and DAPI (nuclei). Scale bars: 20 μ m. (c, d) The content of Ca 2+ and CTX-1 in the coculture medium of osteoclast and bone slices. (e) Representative Western blot analysis and the quantification of NFATc1, c-FOS, CTSK, and MMP9 expression in osteoclast. Images presented are representative of ≥3 sections for each group or independent experiments, and each point represents mean ± SD. ∗ P
    Figure Legend Snippet: OG suppresses bone resorption in SAMP6 mice and osteoclast derived from RAW264.7 cells. (a) Representative Western blot analysis and the quantification of NFATc1, c-FOS, CTSK, and MMP9 expression in bone tissue of SAMP6 mice. (b) Osteoclasts were labeled with phalloidin (F-actin ring) and DAPI (nuclei). Scale bars: 20 μ m. (c, d) The content of Ca 2+ and CTX-1 in the coculture medium of osteoclast and bone slices. (e) Representative Western blot analysis and the quantification of NFATc1, c-FOS, CTSK, and MMP9 expression in osteoclast. Images presented are representative of ≥3 sections for each group or independent experiments, and each point represents mean ± SD. ∗ P

    Techniques Used: Mouse Assay, Derivative Assay, Western Blot, Expressing, Labeling

    7) Product Images from "Clinical value of matrix metalloproteinase-2 and -9 in ultrasound-guided radiofrequency ablation treatment for papillary thyroid carcinoma"

    Article Title: Clinical value of matrix metalloproteinase-2 and -9 in ultrasound-guided radiofrequency ablation treatment for papillary thyroid carcinoma

    Journal: The Journal of International Medical Research

    doi: 10.1177/0300060520917581

    ROC analyses of serum MMP-2 and MMP-9 levels. MMP, matrix metalloproteinase.
    Figure Legend Snippet: ROC analyses of serum MMP-2 and MMP-9 levels. MMP, matrix metalloproteinase.

    Techniques Used:

    8) Product Images from "Pancreatic cancer-derived exosomes promote tumor metastasis and liver pre-metastatic niche formation"

    Article Title: Pancreatic cancer-derived exosomes promote tumor metastasis and liver pre-metastatic niche formation

    Journal: Oncotarget

    doi: 10.18632/oncotarget.18831

    Panc02-H7-derived exosomes promote metastatis-related characteristics in vitro Panc02 cells tookup PKH67-labeled Panc02-H7EXOs. Numerous green fluorescently-labeled exosomes were observed inside cells after 5 h (400× magnification). (A) The MTT cell adhesion assay indicated that Panc02-H7 EXOs decrease Panc02 cell adhesion. (B) Wound-healing assays indicated that Panc02-H7 EXOs enhanced Panc02 cell migration (200×magnification). (C) Transwell chamber invasion assays showed that Panc02-H7 EXOs increased Panc02 cell invasion (200×magnification). (D) Western blotting indicated that Panc02-H7 EXOs increased Panc02 cell migration and invasion via CXCR4 and MMP-9 signaling. (E) n=3/group.*P
    Figure Legend Snippet: Panc02-H7-derived exosomes promote metastatis-related characteristics in vitro Panc02 cells tookup PKH67-labeled Panc02-H7EXOs. Numerous green fluorescently-labeled exosomes were observed inside cells after 5 h (400× magnification). (A) The MTT cell adhesion assay indicated that Panc02-H7 EXOs decrease Panc02 cell adhesion. (B) Wound-healing assays indicated that Panc02-H7 EXOs enhanced Panc02 cell migration (200×magnification). (C) Transwell chamber invasion assays showed that Panc02-H7 EXOs increased Panc02 cell invasion (200×magnification). (D) Western blotting indicated that Panc02-H7 EXOs increased Panc02 cell migration and invasion via CXCR4 and MMP-9 signaling. (E) n=3/group.*P

    Techniques Used: Derivative Assay, In Vitro, Labeling, MTT Assay, Cell Adhesion Assay, Migration, Western Blot

    9) Product Images from "Effects of resveratrol and genistein on nuclear factor-κB, tumor necrosis factor-α and matrix metalloproteinase-9 in patients with chronic obstructive pulmonary disease"

    Article Title: Effects of resveratrol and genistein on nuclear factor-κB, tumor necrosis factor-α and matrix metalloproteinase-9 in patients with chronic obstructive pulmonary disease

    Journal: Molecular Medicine Reports

    doi: 10.3892/mmr.2016.5057

    Effects of Res and Gen on (A) NF-κB, (B) TNF-α and (C) MMP-9 in patients with chronic obstructive pulmonary disease. ** P
    Figure Legend Snippet: Effects of Res and Gen on (A) NF-κB, (B) TNF-α and (C) MMP-9 in patients with chronic obstructive pulmonary disease. ** P

    Techniques Used:

    Concentration-effect curves of resveratrol (upper panels) and genistein (lower panels) on the percentage of (A) NF-κB-positive cells, and the levels of (B) TNF-α and (C) MMP-9 in patients with chronic obstructive pulmonary disease. * P
    Figure Legend Snippet: Concentration-effect curves of resveratrol (upper panels) and genistein (lower panels) on the percentage of (A) NF-κB-positive cells, and the levels of (B) TNF-α and (C) MMP-9 in patients with chronic obstructive pulmonary disease. * P

    Techniques Used: Concentration Assay

    10) Product Images from "Filifactor alocis modulates human neutrophil antimicrobial functional responses"

    Article Title: Filifactor alocis modulates human neutrophil antimicrobial functional responses

    Journal: Cellular microbiology

    doi: 10.1111/cmi.12829

    Opsonized F. alocis induces exocytosis of three of the four neutrophil granule subtypes Neutrophils were unchallenged (basal), challenged with fMLF (5 min), or challenged with live or heat killed (HK) opsonized F. alocis (MOI 10, 30 min). A-B . Secretory vesicle and specific granule exocytosis was determined via flow cytometry by measuring the increase in plasma membrane expression of CD35 or CD66b respectively. Data are expressed as the mean ± SEM of the mean channel of fluorescence (mcf) from 6 independent experiments. In A , ns: non-significant compared to basal. C . Gelatinase granule exocytosis was measured by ELISA as levels of matrix metalloproteinase 9 (MMP-9) present in the supernatants collected from the different experimental conditions. Data are expressed as mean ± SEM of MMP-9 release in ng/4 × 10 6 cells, from 5 independent experiments. D. Gelatin zymography gels were used to determine the activity of MMP-9 in the supernatants from the same experimental conditions described above. The protein standard is shown on the left side, and the different MMP-9 isoforms are also indicated. E. Quantification of the densitometry values of the gel degradation corresponding to the 92 kDa band was analyzed by ImageJ software, from the gels of 4 independent experiments. F. Azurophil granule exocytosis was determined by the increase in plasma membrane expression of the CD63 marker by flow cytometry. Data are expressed as the mean ± SEM of the mean channel of fluorescence (mcf) from 5 independent experiments. *p
    Figure Legend Snippet: Opsonized F. alocis induces exocytosis of three of the four neutrophil granule subtypes Neutrophils were unchallenged (basal), challenged with fMLF (5 min), or challenged with live or heat killed (HK) opsonized F. alocis (MOI 10, 30 min). A-B . Secretory vesicle and specific granule exocytosis was determined via flow cytometry by measuring the increase in plasma membrane expression of CD35 or CD66b respectively. Data are expressed as the mean ± SEM of the mean channel of fluorescence (mcf) from 6 independent experiments. In A , ns: non-significant compared to basal. C . Gelatinase granule exocytosis was measured by ELISA as levels of matrix metalloproteinase 9 (MMP-9) present in the supernatants collected from the different experimental conditions. Data are expressed as mean ± SEM of MMP-9 release in ng/4 × 10 6 cells, from 5 independent experiments. D. Gelatin zymography gels were used to determine the activity of MMP-9 in the supernatants from the same experimental conditions described above. The protein standard is shown on the left side, and the different MMP-9 isoforms are also indicated. E. Quantification of the densitometry values of the gel degradation corresponding to the 92 kDa band was analyzed by ImageJ software, from the gels of 4 independent experiments. F. Azurophil granule exocytosis was determined by the increase in plasma membrane expression of the CD63 marker by flow cytometry. Data are expressed as the mean ± SEM of the mean channel of fluorescence (mcf) from 5 independent experiments. *p

    Techniques Used: Flow Cytometry, Cytometry, Expressing, Fluorescence, Enzyme-linked Immunosorbent Assay, Zymography, Activity Assay, Software, Marker

    11) Product Images from "Elevation of Matrix Metalloproteinase-9 Level in Cerebrospinal Fluid of Tick-Borne Encephalitis Patients Is Associated with IgG Extravassation and Disease Severity"

    Article Title: Elevation of Matrix Metalloproteinase-9 Level in Cerebrospinal Fluid of Tick-Borne Encephalitis Patients Is Associated with IgG Extravassation and Disease Severity

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0077427

    MMP-9 concentrations in TBEV IgG-positive and -negative groups. Diluted MMP-9 standards and CSF samples were added to commercial ELISA plates for MMP-9 detection. After incubation and washing, biotin-conjugated monoclonal antibody was used to bind the MMP-9 in the samples. Horseradish-peroxidase-conjugated avidin was used to amplify the signals. Then a substrate solution capable of reacting with horseradish peroxidase, was added to the wells to produce a color reaction proportional to the amount of MMP-9 present. The MMP-9 concentration in each sample was calculated by comparing it to a MMP-9 standard curve. All of the CSF samples were divided into two groups, TBEV IgG-positive samples and TBEV IgG-negative samples. The difference in the level of MMP-9 expression was analyzed using student t test between the two groups. The limit of detection of this MMP-9 ELISA kit was 1.25 ng/mL.
    Figure Legend Snippet: MMP-9 concentrations in TBEV IgG-positive and -negative groups. Diluted MMP-9 standards and CSF samples were added to commercial ELISA plates for MMP-9 detection. After incubation and washing, biotin-conjugated monoclonal antibody was used to bind the MMP-9 in the samples. Horseradish-peroxidase-conjugated avidin was used to amplify the signals. Then a substrate solution capable of reacting with horseradish peroxidase, was added to the wells to produce a color reaction proportional to the amount of MMP-9 present. The MMP-9 concentration in each sample was calculated by comparing it to a MMP-9 standard curve. All of the CSF samples were divided into two groups, TBEV IgG-positive samples and TBEV IgG-negative samples. The difference in the level of MMP-9 expression was analyzed using student t test between the two groups. The limit of detection of this MMP-9 ELISA kit was 1.25 ng/mL.

    Techniques Used: Enzyme-linked Immunosorbent Assay, Incubation, Avidin-Biotin Assay, Concentration Assay, Expressing

    Correlations among the concentrations of MMP-9 and IL-6. The concentrations of IL-6 and MMP-9 were detected using the commercial ELISA kit, and the detection limits were 1.25 ng/mL for MMP-9 and 0.0375 ng/ml for IL-6. The correlation between the concentrations of MMP-9 and IL-6 was analyzed using Pearson statistics.
    Figure Legend Snippet: Correlations among the concentrations of MMP-9 and IL-6. The concentrations of IL-6 and MMP-9 were detected using the commercial ELISA kit, and the detection limits were 1.25 ng/mL for MMP-9 and 0.0375 ng/ml for IL-6. The correlation between the concentrations of MMP-9 and IL-6 was analyzed using Pearson statistics.

    Techniques Used: Enzyme-linked Immunosorbent Assay

    12) Product Images from "Peptoanaerobacter stomatis Primes Human Neutrophils and Induces Granule Exocytosis"

    Article Title: Peptoanaerobacter stomatis Primes Human Neutrophils and Induces Granule Exocytosis

    Journal: Infection and Immunity

    doi: 10.1128/IAI.01043-16

    (A) P. stomatis induces the exocytosis of secretory vesicles and gelatinase granules. Neutrophils were unchallenged (basal) or challenged with fMLF (5 min), with P. stomatis (30 min), or with P. gingivalis (30 min). (A and B) Secretory vesicle exocytosis was determined by the increase in plasma membrane expression of CD35 by flow cytometry. Data are expressed as the means ± SEM of the mean channel of fluorescence (mcf) from 3 independent experiments. (C) Gelatinase granule exocytosis was measured as levels of matrix metalloproteinase 9 (MMP-9) present in the supernatants collected from the different experimental conditions by ELISA. Data are expressed as means ± SEM of MMP-9 release (in nanograms per 4 × 10 6 cells) from 4 independent experiments. (D) The activities of MMP-9 on the supernatants from the same experimental conditions were analyzed by gelatin zymogen assays. The supernatant from P. stomatis growth media (GS) was also analyzed. The protein standard is shown on the left. MMP-9 isoforms are indicated. (E and F) The densitometry profile of the degraded gelatin zymography 92-kDa band was analyzed by ImageJ software from the gels of 4 independent experiments (E) or from 3 independent experiments (F). *, P
    Figure Legend Snippet: (A) P. stomatis induces the exocytosis of secretory vesicles and gelatinase granules. Neutrophils were unchallenged (basal) or challenged with fMLF (5 min), with P. stomatis (30 min), or with P. gingivalis (30 min). (A and B) Secretory vesicle exocytosis was determined by the increase in plasma membrane expression of CD35 by flow cytometry. Data are expressed as the means ± SEM of the mean channel of fluorescence (mcf) from 3 independent experiments. (C) Gelatinase granule exocytosis was measured as levels of matrix metalloproteinase 9 (MMP-9) present in the supernatants collected from the different experimental conditions by ELISA. Data are expressed as means ± SEM of MMP-9 release (in nanograms per 4 × 10 6 cells) from 4 independent experiments. (D) The activities of MMP-9 on the supernatants from the same experimental conditions were analyzed by gelatin zymogen assays. The supernatant from P. stomatis growth media (GS) was also analyzed. The protein standard is shown on the left. MMP-9 isoforms are indicated. (E and F) The densitometry profile of the degraded gelatin zymography 92-kDa band was analyzed by ImageJ software from the gels of 4 independent experiments (E) or from 3 independent experiments (F). *, P

    Techniques Used: Expressing, Flow Cytometry, Cytometry, Fluorescence, Enzyme-linked Immunosorbent Assay, Zymography, Software

    13) Product Images from "Elevation of Matrix Metalloproteinase-9 Level in Cerebrospinal Fluid of Tick-Borne Encephalitis Patients Is Associated with IgG Extravassation and Disease Severity"

    Article Title: Elevation of Matrix Metalloproteinase-9 Level in Cerebrospinal Fluid of Tick-Borne Encephalitis Patients Is Associated with IgG Extravassation and Disease Severity

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0077427

    MMP-9 concentrations in TBEV IgG-positive and -negative groups. Diluted MMP-9 standards and CSF samples were added to commercial ELISA plates for MMP-9 detection. After incubation and washing, biotin-conjugated monoclonal antibody was used to bind the MMP-9 in the samples. Horseradish-peroxidase-conjugated avidin was used to amplify the signals. Then a substrate solution capable of reacting with horseradish peroxidase, was added to the wells to produce a color reaction proportional to the amount of MMP-9 present. The MMP-9 concentration in each sample was calculated by comparing it to a MMP-9 standard curve. All of the CSF samples were divided into two groups, TBEV IgG-positive samples and TBEV IgG-negative samples. The difference in the level of MMP-9 expression was analyzed using student t test between the two groups. The limit of detection of this MMP-9 ELISA kit was 1.25 ng/mL.
    Figure Legend Snippet: MMP-9 concentrations in TBEV IgG-positive and -negative groups. Diluted MMP-9 standards and CSF samples were added to commercial ELISA plates for MMP-9 detection. After incubation and washing, biotin-conjugated monoclonal antibody was used to bind the MMP-9 in the samples. Horseradish-peroxidase-conjugated avidin was used to amplify the signals. Then a substrate solution capable of reacting with horseradish peroxidase, was added to the wells to produce a color reaction proportional to the amount of MMP-9 present. The MMP-9 concentration in each sample was calculated by comparing it to a MMP-9 standard curve. All of the CSF samples were divided into two groups, TBEV IgG-positive samples and TBEV IgG-negative samples. The difference in the level of MMP-9 expression was analyzed using student t test between the two groups. The limit of detection of this MMP-9 ELISA kit was 1.25 ng/mL.

    Techniques Used: Enzyme-linked Immunosorbent Assay, Incubation, Avidin-Biotin Assay, Concentration Assay, Expressing

    Correlations among the concentrations of MMP-9 and IL-6. The concentrations of IL-6 and MMP-9 were detected using the commercial ELISA kit, and the detection limits were 1.25 ng/mL for MMP-9 and 0.0375 ng/ml for IL-6. The correlation between the concentrations of MMP-9 and IL-6 was analyzed using Pearson statistics.
    Figure Legend Snippet: Correlations among the concentrations of MMP-9 and IL-6. The concentrations of IL-6 and MMP-9 were detected using the commercial ELISA kit, and the detection limits were 1.25 ng/mL for MMP-9 and 0.0375 ng/ml for IL-6. The correlation between the concentrations of MMP-9 and IL-6 was analyzed using Pearson statistics.

    Techniques Used: Enzyme-linked Immunosorbent Assay

    14) Product Images from "Thalidomide influences growth and vasculogenic mimicry channel formation in melanoma"

    Article Title: Thalidomide influences growth and vasculogenic mimicry channel formation in melanoma

    Journal: Journal of Experimental & Clinical Cancer Research : CR

    doi: 10.1186/1756-9966-27-60

    Immunohistochemical staining results of VEGF, NF-κB, MMP-2, MMP-9, and PCNA. VEGF, NF-κB, PCNA, MMP-2, MMP-9 protein expression including the number and staining intensity of positive cells in the treatment group were significantly lower than those in the control groups. A. VEGF expression in the control group. Tumor cells showed brown cytoplasmic staining. IHC, 400×. B. VEGF expression in the treatment group. IHC, 400×. C. NF-κB expression in the control group. Tumor cells showed brown cytoplasmic staining. The immune reaction in the nuclear was rare in this study. IHC, 400×. D. NF-κB expression in the treatment group. IHC, 400×. E. MMP-2 expression in the control group. Tumor cells showed brown cytoplasmic staining. IHC, 200×. F. MMP-2 expression in the treatment group. IHC, 200×. G. MMP-9 expression in the control group. Tumor cells showed brown cytoplasmic staining. IHC, 200×. H. MMP-9 expression in the treatment group. IHC, 200×. I. PCNA expression in the control group. Tumor cells showed brown nuclear staining. IHC, 200×. J: PCNA expression in the treatment group. IHC, 200×.
    Figure Legend Snippet: Immunohistochemical staining results of VEGF, NF-κB, MMP-2, MMP-9, and PCNA. VEGF, NF-κB, PCNA, MMP-2, MMP-9 protein expression including the number and staining intensity of positive cells in the treatment group were significantly lower than those in the control groups. A. VEGF expression in the control group. Tumor cells showed brown cytoplasmic staining. IHC, 400×. B. VEGF expression in the treatment group. IHC, 400×. C. NF-κB expression in the control group. Tumor cells showed brown cytoplasmic staining. The immune reaction in the nuclear was rare in this study. IHC, 400×. D. NF-κB expression in the treatment group. IHC, 400×. E. MMP-2 expression in the control group. Tumor cells showed brown cytoplasmic staining. IHC, 200×. F. MMP-2 expression in the treatment group. IHC, 200×. G. MMP-9 expression in the control group. Tumor cells showed brown cytoplasmic staining. IHC, 200×. H. MMP-9 expression in the treatment group. IHC, 200×. I. PCNA expression in the control group. Tumor cells showed brown nuclear staining. IHC, 200×. J: PCNA expression in the treatment group. IHC, 200×.

    Techniques Used: Immunohistochemistry, Staining, Expressing

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  • 86
    Boster Bio mmp 9
    FOXP3 promotes the migratory and invasive abilities of A549 cells. (A) Reverse transcription quantitative PCR assessing the efficacy of FOXP3 knockdown using specific siRNAs in A549 cells. (B) Western blotting assessing the efficacy of FOXP3 knockdown using specific siRNAs in A549 cells. (C) Transwell assays assessing the cell migratory and invasive abilities of A549 following FOXP3 knockdown. (D and E) ELISA detection of MMP-2 and <t>MMP-9</t> secretion from A549 cells 72 h following FOXP3 knockdown. ** P
    Mmp 9, supplied by Boster Bio, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mmp 9/product/Boster Bio
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mmp 9 - by Bioz Stars, 2022-07
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    93
    Boster Bio anti mmp 9
    The effects of IHH on MMP-2/-9/-14 and TIMP-2 expression in aortas from ApoE -/- mice. (A) Representative immunohistochemical stains showing the expression of MMP-2, <t>MMP-9,</t> MMP-14, and TIMP-1 in the aorta. (B) The levels of protein expression were expressed
    Anti Mmp 9, supplied by Boster Bio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti mmp 9/product/Boster Bio
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti mmp 9 - by Bioz Stars, 2022-07
    93/100 stars
      Buy from Supplier

    91
    Boster Bio mouse anti human mmp 9 monoclonal antibody
    <t>MMP-9</t> staining of gliomas. MMP-9 was detected in the cytoplasm of tumor cells (brown, short arrow), as well as the cytoplasm of vascular endothelial cells (brown particles, long arrow). (A) High-grade and (B) low-grade glioma.
    Mouse Anti Human Mmp 9 Monoclonal Antibody, supplied by Boster Bio, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti human mmp 9 monoclonal antibody/product/Boster Bio
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse anti human mmp 9 monoclonal antibody - by Bioz Stars, 2022-07
    91/100 stars
      Buy from Supplier

    Image Search Results


    FOXP3 promotes the migratory and invasive abilities of A549 cells. (A) Reverse transcription quantitative PCR assessing the efficacy of FOXP3 knockdown using specific siRNAs in A549 cells. (B) Western blotting assessing the efficacy of FOXP3 knockdown using specific siRNAs in A549 cells. (C) Transwell assays assessing the cell migratory and invasive abilities of A549 following FOXP3 knockdown. (D and E) ELISA detection of MMP-2 and MMP-9 secretion from A549 cells 72 h following FOXP3 knockdown. ** P

    Journal: Experimental and Therapeutic Medicine

    Article Title: FOXP3 facilitates the invasion and metastasis of non-small cell lung cancer cells through regulating VEGF, EMT and the Notch1/Hes1 pathway

    doi: 10.3892/etm.2021.10390

    Figure Lengend Snippet: FOXP3 promotes the migratory and invasive abilities of A549 cells. (A) Reverse transcription quantitative PCR assessing the efficacy of FOXP3 knockdown using specific siRNAs in A549 cells. (B) Western blotting assessing the efficacy of FOXP3 knockdown using specific siRNAs in A549 cells. (C) Transwell assays assessing the cell migratory and invasive abilities of A549 following FOXP3 knockdown. (D and E) ELISA detection of MMP-2 and MMP-9 secretion from A549 cells 72 h following FOXP3 knockdown. ** P

    Article Snippet: The concentrations of matrix metalloproteinase-2 (MMP-2), MMP-9 and VEGF in the culture supernatant were detected using ELISA kits (cat. no. EK0459; cat. no. EK0465; cat. no. EK0539; Boster Biological Technology) according to the manufacturers' protocol.

    Techniques: Real-time Polymerase Chain Reaction, Western Blot, Enzyme-linked Immunosorbent Assay

    Inhibition of factors involved in inflammatory processes by rubriflordilactone treatment in LPS administered MLE-15 cells. In MLE-15 cells LPS exposure increased expression of MMP-9, however, rubriflordilactone treated suppressed the effect of LPS onMMP-9 expression. Rubriflordilactone inhibited the LPS induced increase in the expression of mRNA corresponding to iNOS, MMP-9 and IL-6. Rubriflordilactone inhibited the LPS induced reduction in the expression of Sirt1 in MLE-15 cells. Rub, LPS, Sirt1, MMP-9, IL and iNOS stands for rubriflordilactone, lipopolysaccharide, sirtuin 1, matrix metalloproteinase-9, interleukin and inducible nitric oxide synthase.

    Journal: International Journal of Clinical and Experimental Pathology

    Article Title: Inhibition of acute lung injury by rubriflordilactone in LPS-induced rat model through suppression of inflammatory factor expression

    doi:

    Figure Lengend Snippet: Inhibition of factors involved in inflammatory processes by rubriflordilactone treatment in LPS administered MLE-15 cells. In MLE-15 cells LPS exposure increased expression of MMP-9, however, rubriflordilactone treated suppressed the effect of LPS onMMP-9 expression. Rubriflordilactone inhibited the LPS induced increase in the expression of mRNA corresponding to iNOS, MMP-9 and IL-6. Rubriflordilactone inhibited the LPS induced reduction in the expression of Sirt1 in MLE-15 cells. Rub, LPS, Sirt1, MMP-9, IL and iNOS stands for rubriflordilactone, lipopolysaccharide, sirtuin 1, matrix metalloproteinase-9, interleukin and inducible nitric oxide synthase.

    Article Snippet: In consistence with these reports, our results showed a marked increase in the expression level of MMP-9 in LPS administered rats.

    Techniques: Inhibition, Expressing

    Effect of silencer RNA for Sirt1 on inhibition of acute lung injury by rubriflordilactone inMLE-15 cells. The cells transfected with siRNA for Sirt1 were exposed to LPS after rubriflordilactone treatment and then analyzed by western blot analysis. Rub, LPS, Sirt1, MMP-9, IL and iNOS stand for rubriflordilactone, lipopolysaccharide, sirtuin 1, matrix metalloproteinase-9, interleukin and inducible nitric oxide synthase.

    Journal: International Journal of Clinical and Experimental Pathology

    Article Title: Inhibition of acute lung injury by rubriflordilactone in LPS-induced rat model through suppression of inflammatory factor expression

    doi:

    Figure Lengend Snippet: Effect of silencer RNA for Sirt1 on inhibition of acute lung injury by rubriflordilactone inMLE-15 cells. The cells transfected with siRNA for Sirt1 were exposed to LPS after rubriflordilactone treatment and then analyzed by western blot analysis. Rub, LPS, Sirt1, MMP-9, IL and iNOS stand for rubriflordilactone, lipopolysaccharide, sirtuin 1, matrix metalloproteinase-9, interleukin and inducible nitric oxide synthase.

    Article Snippet: In consistence with these reports, our results showed a marked increase in the expression level of MMP-9 in LPS administered rats.

    Techniques: Inhibition, Transfection, Western Blot

    The effects of IHH on MMP-2/-9/-14 and TIMP-2 expression in aortas from ApoE -/- mice. (A) Representative immunohistochemical stains showing the expression of MMP-2, MMP-9, MMP-14, and TIMP-1 in the aorta. (B) The levels of protein expression were expressed

    Journal: High Altitude Medicine & Biology

    Article Title: Intermittent Hypobaric Hypoxia Promotes Atherosclerotic Plaque Instability in ApoE-Deficient Mice

    doi: 10.1089/ham.2012.1083

    Figure Lengend Snippet: The effects of IHH on MMP-2/-9/-14 and TIMP-2 expression in aortas from ApoE -/- mice. (A) Representative immunohistochemical stains showing the expression of MMP-2, MMP-9, MMP-14, and TIMP-1 in the aorta. (B) The levels of protein expression were expressed

    Article Snippet: The sections were incubated with anti-MMP-2, anti-MMP-3, anti-MMP-9, anti-MMP-14, anti-TIMP-1, and anti-TIMP-2 antibodies (diluted in 1:50 dilution, Boster Bioengineering Co., Wuhan, China).

    Techniques: Expressing, Mouse Assay, Immunohistochemistry

    MMP-9 staining of gliomas. MMP-9 was detected in the cytoplasm of tumor cells (brown, short arrow), as well as the cytoplasm of vascular endothelial cells (brown particles, long arrow). (A) High-grade and (B) low-grade glioma.

    Journal: Oncology Letters

    Article Title: Expression of VEGF and MMP-9 and MRI imaging changes in cerebral glioma

    doi: 10.3892/ol.2011.384

    Figure Lengend Snippet: MMP-9 staining of gliomas. MMP-9 was detected in the cytoplasm of tumor cells (brown, short arrow), as well as the cytoplasm of vascular endothelial cells (brown particles, long arrow). (A) High-grade and (B) low-grade glioma.

    Article Snippet: Other sections were used to perform immunohistochemical staining with mouse anti-human VEGF monoclonal antibody and mouse anti-human MMP-9 monoclonal antibody (Wuhan Boster Biological Technology Co., Ltd., China) detected by SP or DAB kits, according to the manufacturer’s instructions (Wuhan Boster Biological Technology Co., Ltd.).

    Techniques: Staining

    Correlation between VEGF and MMP-9 expression in gliomas and peritumoral EI, EP and tumor size

    Journal: Oncology Letters

    Article Title: Expression of VEGF and MMP-9 and MRI imaging changes in cerebral glioma

    doi: 10.3892/ol.2011.384

    Figure Lengend Snippet: Correlation between VEGF and MMP-9 expression in gliomas and peritumoral EI, EP and tumor size

    Article Snippet: Other sections were used to perform immunohistochemical staining with mouse anti-human VEGF monoclonal antibody and mouse anti-human MMP-9 monoclonal antibody (Wuhan Boster Biological Technology Co., Ltd., China) detected by SP or DAB kits, according to the manufacturer’s instructions (Wuhan Boster Biological Technology Co., Ltd.).

    Techniques: Expressing