Structured Review

New England Biolabs mmei
Mmei, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mmei/product/New England Biolabs
Average 99 stars, based on 30 article reviews
Price from $9.99 to $1999.99
mmei - by Bioz Stars, 2020-01
99/100 stars

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Polymerase Chain Reaction:

Article Title: Rapid identification of heterozygous mutations in Drosophila melanogaster using genomic capture sequencing
Article Snippet: .. To prepare Illumina libraries, purified PCR amplicons were first digested with MmeI: 16 units of MmeI (2 U/μL; New England BioLabs), 100 μM SAM in 1× New England BioLabs Buffer 4 at 37°C for 1 h. Digestions were again column purified and digested with 3 U of USER enzyme (1 U/μL; New England BioLabs) at 37°C for 2 h, then with 10 U of S1 nuclease (10 U/μL; Invitrogen) in 1× S1 nuclease buffer at 37°C for 10 min. .. The fragmented DNA was column purified and used as DNA template for the Illumina library.

Generated:

Article Title: Rapid identification of heterozygous mutations in Drosophila melanogaster using genomic capture sequencing
Article Snippet: To prepare Illumina libraries, purified PCR amplicons were first digested with MmeI: 16 units of MmeI (2 U/μL; New England BioLabs), 100 μM SAM in 1× New England BioLabs Buffer 4 at 37°C for 1 h. Digestions were again column purified and digested with 3 U of USER enzyme (1 U/μL; New England BioLabs) at 37°C for 2 h, then with 10 U of S1 nuclease (10 U/μL; Invitrogen) in 1× S1 nuclease buffer at 37°C for 10 min. .. Illumina libraries were generated by following the Illumina pair-end library preparation protocol.

Purification:

Article Title: Rapid identification of heterozygous mutations in Drosophila melanogaster using genomic capture sequencing
Article Snippet: .. To prepare Illumina libraries, purified PCR amplicons were first digested with MmeI: 16 units of MmeI (2 U/μL; New England BioLabs), 100 μM SAM in 1× New England BioLabs Buffer 4 at 37°C for 1 h. Digestions were again column purified and digested with 3 U of USER enzyme (1 U/μL; New England BioLabs) at 37°C for 2 h, then with 10 U of S1 nuclease (10 U/μL; Invitrogen) in 1× S1 nuclease buffer at 37°C for 10 min. .. The fragmented DNA was column purified and used as DNA template for the Illumina library.

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    New England Biolabs enzymes sfi1 mme1
    Enzymes Sfi1 Mme1, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 90/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/enzymes sfi1 mme1/product/New England Biolabs
    Average 90 stars, based on 14 article reviews
    Price from $9.99 to $1999.99
    enzymes sfi1 mme1 - by Bioz Stars, 2020-01
    90/100 stars
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