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Illumina Inc mme i
Mme I, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mme i/product/Illumina Inc
Average 84 stars, based on 1 article reviews
Price from $9.99 to $1999.99
mme i - by Bioz Stars, 2019-10
84/100 stars

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Flow Cytometry:

Article Title: Transcriptome assembly and expression profiling of molecular responses to cadmium toxicity in hepatopancreas of the freshwater crab Sinopotamon henanense
Article Snippet: Subsequently, the bead-bound cDNA was digested with Nla III and added Illumina adapter 1, and then digested with Mme I and added Illumina adapter 2 to create the sequencing library. .. After amplification by PCR, cDNA tag library was purified and checked.

Amplification:

Article Title: Histone methylation mediates plasticity of human FOXP3+ regulatory T cells by modulating signature gene expressions
Article Snippet: The resultant cDNA was endonuclease digested ( Nla III), which recognizes and cuts the most 3′ ‘CATG’, and ligated to Illumina adapter 1. .. Following a sequential digestion of the 5′ end with Mme I and ligation to Illumina adapter 2, the DNA fragments (library) were amplified by PCR, gel purified and sequenced on the HiSeq™ 2000 system. .. Raw image data were transformed by base calling into raw sequence data and then transformed into clean tags for alignment to the human reference genome hg19.

Article Title: Transcriptome assembly and expression profiling of molecular responses to cadmium toxicity in hepatopancreas of the freshwater crab Sinopotamon henanense
Article Snippet: Subsequently, the bead-bound cDNA was digested with Nla III and added Illumina adapter 1, and then digested with Mme I and added Illumina adapter 2 to create the sequencing library. .. After amplification by PCR, cDNA tag library was purified and checked.

Sample Prep:

Article Title: Exome Capture Sequencing of Adenoma Reveals Genetic Alterations in Multiple Cellular Pathways at the Early Stage of Colorectal Tumorigenesis
Article Snippet: The libraries for sequencing tags were prepared using Illumina Gene Expression Sample Prep Kit (Illumina, CA, USA) according to manufacturer’s instruction. .. Mme I (an endonuclease provided by Illumina with a different recognition site and a digestion site) cut at 17 bp downstream of the CATG site and subsequently produced tags with adaptor 1.

In Situ:

Article Title: Transcriptome assembly and expression profiling of molecular responses to cadmium toxicity in hepatopancreas of the freshwater crab Sinopotamon henanense
Article Snippet: Subsequently, the bead-bound cDNA was digested with Nla III and added Illumina adapter 1, and then digested with Mme I and added Illumina adapter 2 to create the sequencing library. .. After amplification by PCR, cDNA tag library was purified and checked.

Magnetic Beads:

Article Title: Exome Capture Sequencing of Adenoma Reveals Genetic Alterations in Multiple Cellular Pathways at the Early Stage of Colorectal Tumorigenesis
Article Snippet: Briefly, Messenger RNA was purified using Oligo(dT) magnetic beads (Illumina, CA, USA) from total RNA. .. Mme I (an endonuclease provided by Illumina with a different recognition site and a digestion site) cut at 17 bp downstream of the CATG site and subsequently produced tags with adaptor 1.

Article Title: Histone methylation mediates plasticity of human FOXP3+ regulatory T cells by modulating signature gene expressions
Article Snippet: Briefly, mRNA was first isolated from the total RNA by using Oligo(dT) magnetic beads and then applied as template for cDNA synthesis. .. Following a sequential digestion of the 5′ end with Mme I and ligation to Illumina adapter 2, the DNA fragments (library) were amplified by PCR, gel purified and sequenced on the HiSeq™ 2000 system.

Article Title: Transcriptome assembly and expression profiling of molecular responses to cadmium toxicity in hepatopancreas of the freshwater crab Sinopotamon henanense
Article Snippet: In brief, each isolated mRNA sample was respectively enriched by oligo-dT magnetic beads, and prepared for synthesizing double stranded cDNA. .. Subsequently, the bead-bound cDNA was digested with Nla III and added Illumina adapter 1, and then digested with Mme I and added Illumina adapter 2 to create the sequencing library.

Isolation:

Article Title: Histone methylation mediates plasticity of human FOXP3+ regulatory T cells by modulating signature gene expressions
Article Snippet: Briefly, mRNA was first isolated from the total RNA by using Oligo(dT) magnetic beads and then applied as template for cDNA synthesis. .. Following a sequential digestion of the 5′ end with Mme I and ligation to Illumina adapter 2, the DNA fragments (library) were amplified by PCR, gel purified and sequenced on the HiSeq™ 2000 system.

Article Title: Transcriptome assembly and expression profiling of molecular responses to cadmium toxicity in hepatopancreas of the freshwater crab Sinopotamon henanense
Article Snippet: In brief, each isolated mRNA sample was respectively enriched by oligo-dT magnetic beads, and prepared for synthesizing double stranded cDNA. .. Subsequently, the bead-bound cDNA was digested with Nla III and added Illumina adapter 1, and then digested with Mme I and added Illumina adapter 2 to create the sequencing library.

Synthesized:

Article Title: Exome Capture Sequencing of Adenoma Reveals Genetic Alterations in Multiple Cellular Pathways at the Early Stage of Colorectal Tumorigenesis
Article Snippet: The first and second-strand cDNA were synthesized using Oligo(dT) as the primer. .. Mme I (an endonuclease provided by Illumina with a different recognition site and a digestion site) cut at 17 bp downstream of the CATG site and subsequently produced tags with adaptor 1.

Ligation:

Article Title: Histone methylation mediates plasticity of human FOXP3+ regulatory T cells by modulating signature gene expressions
Article Snippet: The resultant cDNA was endonuclease digested ( Nla III), which recognizes and cuts the most 3′ ‘CATG’, and ligated to Illumina adapter 1. .. Following a sequential digestion of the 5′ end with Mme I and ligation to Illumina adapter 2, the DNA fragments (library) were amplified by PCR, gel purified and sequenced on the HiSeq™ 2000 system. .. Raw image data were transformed by base calling into raw sequence data and then transformed into clean tags for alignment to the human reference genome hg19.

Construct:

Article Title: Transcriptome assembly and expression profiling of molecular responses to cadmium toxicity in hepatopancreas of the freshwater crab Sinopotamon henanense
Article Snippet: The separately stored 4 RNA samples from the control group and three Cd treatment groups (n = 3 in each group) were respectively used to isolate the mRNAs by oligo (dT) beads and Oligotex mRNA Kits (Qiagen, Valencia, CA, USA), and the isolated mRNAs were used to construct 4 differential cDNA libraries in parallel with Digital Gene Expression (DGE) tag profile kit (Illumina, San Diego, CA, USA), according to the manufacturer’s protocol (Version 2.1B). .. Subsequently, the bead-bound cDNA was digested with Nla III and added Illumina adapter 1, and then digested with Mme I and added Illumina adapter 2 to create the sequencing library.

Purification:

Article Title: Exome Capture Sequencing of Adenoma Reveals Genetic Alterations in Multiple Cellular Pathways at the Early Stage of Colorectal Tumorigenesis
Article Snippet: Briefly, Messenger RNA was purified using Oligo(dT) magnetic beads (Illumina, CA, USA) from total RNA. .. Mme I (an endonuclease provided by Illumina with a different recognition site and a digestion site) cut at 17 bp downstream of the CATG site and subsequently produced tags with adaptor 1.

Article Title: Histone methylation mediates plasticity of human FOXP3+ regulatory T cells by modulating signature gene expressions
Article Snippet: The resultant cDNA was endonuclease digested ( Nla III), which recognizes and cuts the most 3′ ‘CATG’, and ligated to Illumina adapter 1. .. Following a sequential digestion of the 5′ end with Mme I and ligation to Illumina adapter 2, the DNA fragments (library) were amplified by PCR, gel purified and sequenced on the HiSeq™ 2000 system. .. Raw image data were transformed by base calling into raw sequence data and then transformed into clean tags for alignment to the human reference genome hg19.

Produced:

Article Title: Exome Capture Sequencing of Adenoma Reveals Genetic Alterations in Multiple Cellular Pathways at the Early Stage of Colorectal Tumorigenesis
Article Snippet: Then, the Illumina adaptor 1 was ligated to the sticky 5' end of the digested fragments. .. Mme I (an endonuclease provided by Illumina with a different recognition site and a digestion site) cut at 17 bp downstream of the CATG site and subsequently produced tags with adaptor 1. .. After removing 3' fragments, Illumina adaptor 2 was ligated to the 3' ends of tags, thereby acquiring tags with different adaptors at both ends to form a tag library.

Sequencing:

Article Title: Exome Capture Sequencing of Adenoma Reveals Genetic Alterations in Multiple Cellular Pathways at the Early Stage of Colorectal Tumorigenesis
Article Snippet: The libraries for sequencing tags were prepared using Illumina Gene Expression Sample Prep Kit (Illumina, CA, USA) according to manufacturer’s instruction. .. Mme I (an endonuclease provided by Illumina with a different recognition site and a digestion site) cut at 17 bp downstream of the CATG site and subsequently produced tags with adaptor 1.

Article Title: Histone methylation mediates plasticity of human FOXP3+ regulatory T cells by modulating signature gene expressions
Article Snippet: Total RNA was extracted from fixed cells using High pure FFPE RNA Micro Kit (Roche Diagnostic Corp., Indianapolis, IN) and submitted to BGI-Shenzhen (China) for lllumina sequencing. .. Following a sequential digestion of the 5′ end with Mme I and ligation to Illumina adapter 2, the DNA fragments (library) were amplified by PCR, gel purified and sequenced on the HiSeq™ 2000 system.

Article Title: Transcriptome assembly and expression profiling of molecular responses to cadmium toxicity in hepatopancreas of the freshwater crab Sinopotamon henanense
Article Snippet: In brief, each isolated mRNA sample was respectively enriched by oligo-dT magnetic beads, and prepared for synthesizing double stranded cDNA. .. Subsequently, the bead-bound cDNA was digested with Nla III and added Illumina adapter 1, and then digested with Mme I and added Illumina adapter 2 to create the sequencing library. .. After amplification by PCR, cDNA tag library was purified and checked.

Formalin-fixed Paraffin-Embedded:

Article Title: Histone methylation mediates plasticity of human FOXP3+ regulatory T cells by modulating signature gene expressions
Article Snippet: Total RNA was extracted from fixed cells using High pure FFPE RNA Micro Kit (Roche Diagnostic Corp., Indianapolis, IN) and submitted to BGI-Shenzhen (China) for lllumina sequencing. .. Following a sequential digestion of the 5′ end with Mme I and ligation to Illumina adapter 2, the DNA fragments (library) were amplified by PCR, gel purified and sequenced on the HiSeq™ 2000 system.

Expressing:

Article Title: Exome Capture Sequencing of Adenoma Reveals Genetic Alterations in Multiple Cellular Pathways at the Early Stage of Colorectal Tumorigenesis
Article Snippet: Paragraph title: Digital Gene Expression Profiling ... Mme I (an endonuclease provided by Illumina with a different recognition site and a digestion site) cut at 17 bp downstream of the CATG site and subsequently produced tags with adaptor 1.

Article Title: Histone methylation mediates plasticity of human FOXP3+ regulatory T cells by modulating signature gene expressions
Article Snippet: Paragraph title: DGE and differential expression analysis ... Following a sequential digestion of the 5′ end with Mme I and ligation to Illumina adapter 2, the DNA fragments (library) were amplified by PCR, gel purified and sequenced on the HiSeq™ 2000 system.

Article Title: Transcriptome assembly and expression profiling of molecular responses to cadmium toxicity in hepatopancreas of the freshwater crab Sinopotamon henanense
Article Snippet: Paragraph title: Differential expression library construction, gene expression profile sequencing and annotation ... Subsequently, the bead-bound cDNA was digested with Nla III and added Illumina adapter 1, and then digested with Mme I and added Illumina adapter 2 to create the sequencing library.

Polymerase Chain Reaction:

Article Title: Histone methylation mediates plasticity of human FOXP3+ regulatory T cells by modulating signature gene expressions
Article Snippet: The resultant cDNA was endonuclease digested ( Nla III), which recognizes and cuts the most 3′ ‘CATG’, and ligated to Illumina adapter 1. .. Following a sequential digestion of the 5′ end with Mme I and ligation to Illumina adapter 2, the DNA fragments (library) were amplified by PCR, gel purified and sequenced on the HiSeq™ 2000 system. .. Raw image data were transformed by base calling into raw sequence data and then transformed into clean tags for alignment to the human reference genome hg19.

Transformation Assay:

Article Title: Transcriptome assembly and expression profiling of molecular responses to cadmium toxicity in hepatopancreas of the freshwater crab Sinopotamon henanense
Article Snippet: Subsequently, the bead-bound cDNA was digested with Nla III and added Illumina adapter 1, and then digested with Mme I and added Illumina adapter 2 to create the sequencing library. .. Subsequently, the bead-bound cDNA was digested with Nla III and added Illumina adapter 1, and then digested with Mme I and added Illumina adapter 2 to create the sequencing library.

Chromatin Immunoprecipitation:

Article Title: Transcriptome assembly and expression profiling of molecular responses to cadmium toxicity in hepatopancreas of the freshwater crab Sinopotamon henanense
Article Snippet: Subsequently, the bead-bound cDNA was digested with Nla III and added Illumina adapter 1, and then digested with Mme I and added Illumina adapter 2 to create the sequencing library. .. After amplification by PCR, cDNA tag library was purified and checked.

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    Illumina Inc mme i
    Mme I, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 84/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mme i/product/Illumina Inc
    Average 84 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    mme i - by Bioz Stars, 2019-10
    84/100 stars
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