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Illumina Inc mme i
Mme I, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mme i/product/Illumina Inc
Average 88 stars, based on 1 article reviews
Price from $9.99 to $1999.99
mme i - by Bioz Stars, 2020-05
88/100 stars

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Related Articles

Amplification:

Article Title: Histone methylation mediates plasticity of human FOXP3+ regulatory T cells by modulating signature gene expressions
Article Snippet: .. Following a sequential digestion of the 5′ end with Mme I and ligation to Illumina adapter 2, the DNA fragments (library) were amplified by PCR, gel purified and sequenced on the HiSeq™ 2000 system. .. Raw image data were transformed by base calling into raw sequence data and then transformed into clean tags for alignment to the human reference genome hg19.

Ligation:

Article Title: Histone methylation mediates plasticity of human FOXP3+ regulatory T cells by modulating signature gene expressions
Article Snippet: .. Following a sequential digestion of the 5′ end with Mme I and ligation to Illumina adapter 2, the DNA fragments (library) were amplified by PCR, gel purified and sequenced on the HiSeq™ 2000 system. .. Raw image data were transformed by base calling into raw sequence data and then transformed into clean tags for alignment to the human reference genome hg19.

Construct:

Article Title: Transcriptome profiling reveals the genetic basis of alkalinity tolerance in wheat
Article Snippet: .. The fragments were further digested with Mme I (this enzyme cleaves 17 bp downstream of the CATG site), then ligated to Illumina adaptor 2 to construct a 21 bp tag library with different adaptors at both ends. .. After linear PCR amplification based on the primer pair corresponding to the adaptors, the amplified samples were purified by polyacrylamide gel electrophoresis.

Purification:

Article Title: Histone methylation mediates plasticity of human FOXP3+ regulatory T cells by modulating signature gene expressions
Article Snippet: .. Following a sequential digestion of the 5′ end with Mme I and ligation to Illumina adapter 2, the DNA fragments (library) were amplified by PCR, gel purified and sequenced on the HiSeq™ 2000 system. .. Raw image data were transformed by base calling into raw sequence data and then transformed into clean tags for alignment to the human reference genome hg19.

Produced:

Article Title: Exome Capture Sequencing of Adenoma Reveals Genetic Alterations in Multiple Cellular Pathways at the Early Stage of Colorectal Tumorigenesis
Article Snippet: .. Mme I (an endonuclease provided by Illumina with a different recognition site and a digestion site) cut at 17 bp downstream of the CATG site and subsequently produced tags with adaptor 1. .. After removing 3' fragments, Illumina adaptor 2 was ligated to the 3' ends of tags, thereby acquiring tags with different adaptors at both ends to form a tag library.

Sequencing:

Article Title: Transcriptome analysis of stem development in the tumourous stem mustard Brassica juncea var. tumida Tsen et Lee by RNA sequencing
Article Snippet: .. After digestion with Mme I, which recognises the junction between Illumina adaptor 1 and the sequence CATG, 21-bp tags containing adaptor 2 were ligated to the 3' ends of the tags to create a tag library. .. The library was amplified by PCR over 15 cycles, and 85-bp strips were purified by PAGE.

Article Title: Transcriptome assembly and expression profiling of molecular responses to cadmium toxicity in hepatopancreas of the freshwater crab Sinopotamon henanense
Article Snippet: .. Subsequently, the bead-bound cDNA was digested with Nla III and added Illumina adapter 1, and then digested with Mme I and added Illumina adapter 2 to create the sequencing library. .. After amplification by PCR, cDNA tag library was purified and checked.

Polymerase Chain Reaction:

Article Title: Histone methylation mediates plasticity of human FOXP3+ regulatory T cells by modulating signature gene expressions
Article Snippet: .. Following a sequential digestion of the 5′ end with Mme I and ligation to Illumina adapter 2, the DNA fragments (library) were amplified by PCR, gel purified and sequenced on the HiSeq™ 2000 system. .. Raw image data were transformed by base calling into raw sequence data and then transformed into clean tags for alignment to the human reference genome hg19.

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  • 88
    Illumina Inc mme i digestion
    Schematic of ChIA-PET analysis . (a) The ChIA-PET experimental protocol, which includes chromatin preparation, ChIP, linker ligation, proximity ligation, <t>Mme</t> I restriction digestion, and DNA sequencing. (b) The ChIA-PET constructs prepared for sequencing analysis. Each PET construct involves a pair of tags (20 bp each) and a linker (38 bp) between the tag pairs. This full-length linker is derived from ligation of two half-linkers, A or B, each with a unique barcode nucleotide (CG for half-linker A and AT for half-linker B). The barcode nucleotides are highlighted as red letters. Linkers with AB barcodes are considered to be non-specific chimeric proximity ligation products. (c) Mapping tags of PET sequences to reference genome. The categories of 'self-ligation PETs' and 'inter-ligation PETs' were assigned. (d) Clustering of overlapping PET sequences in the same genomic regions to identify enriched protein binding sites by overlapping 'self-ligation PETs' and long-range chromatin interactions by overlapping 'inter-ligation PETs'.
    Mme I Digestion, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 88/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mme i digestion/product/Illumina Inc
    Average 88 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    mme i digestion - by Bioz Stars, 2020-05
    88/100 stars
      Buy from Supplier

    88
    Illumina Inc mme i
    Schematic of ChIA-PET analysis . (a) The ChIA-PET experimental protocol, which includes chromatin preparation, ChIP, linker ligation, proximity ligation, <t>Mme</t> I restriction digestion, and DNA sequencing. (b) The ChIA-PET constructs prepared for sequencing analysis. Each PET construct involves a pair of tags (20 bp each) and a linker (38 bp) between the tag pairs. This full-length linker is derived from ligation of two half-linkers, A or B, each with a unique barcode nucleotide (CG for half-linker A and AT for half-linker B). The barcode nucleotides are highlighted as red letters. Linkers with AB barcodes are considered to be non-specific chimeric proximity ligation products. (c) Mapping tags of PET sequences to reference genome. The categories of 'self-ligation PETs' and 'inter-ligation PETs' were assigned. (d) Clustering of overlapping PET sequences in the same genomic regions to identify enriched protein binding sites by overlapping 'self-ligation PETs' and long-range chromatin interactions by overlapping 'inter-ligation PETs'.
    Mme I, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mme i/product/Illumina Inc
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mme i - by Bioz Stars, 2020-05
    88/100 stars
      Buy from Supplier

    Image Search Results


    Schematic of ChIA-PET analysis . (a) The ChIA-PET experimental protocol, which includes chromatin preparation, ChIP, linker ligation, proximity ligation, Mme I restriction digestion, and DNA sequencing. (b) The ChIA-PET constructs prepared for sequencing analysis. Each PET construct involves a pair of tags (20 bp each) and a linker (38 bp) between the tag pairs. This full-length linker is derived from ligation of two half-linkers, A or B, each with a unique barcode nucleotide (CG for half-linker A and AT for half-linker B). The barcode nucleotides are highlighted as red letters. Linkers with AB barcodes are considered to be non-specific chimeric proximity ligation products. (c) Mapping tags of PET sequences to reference genome. The categories of 'self-ligation PETs' and 'inter-ligation PETs' were assigned. (d) Clustering of overlapping PET sequences in the same genomic regions to identify enriched protein binding sites by overlapping 'self-ligation PETs' and long-range chromatin interactions by overlapping 'inter-ligation PETs'.

    Journal: Genome Biology

    Article Title: ChIA-PET tool for comprehensive chromatin interaction analysis with paired-end tag sequencing

    doi: 10.1186/gb-2010-11-2-r22

    Figure Lengend Snippet: Schematic of ChIA-PET analysis . (a) The ChIA-PET experimental protocol, which includes chromatin preparation, ChIP, linker ligation, proximity ligation, Mme I restriction digestion, and DNA sequencing. (b) The ChIA-PET constructs prepared for sequencing analysis. Each PET construct involves a pair of tags (20 bp each) and a linker (38 bp) between the tag pairs. This full-length linker is derived from ligation of two half-linkers, A or B, each with a unique barcode nucleotide (CG for half-linker A and AT for half-linker B). The barcode nucleotides are highlighted as red letters. Linkers with AB barcodes are considered to be non-specific chimeric proximity ligation products. (c) Mapping tags of PET sequences to reference genome. The categories of 'self-ligation PETs' and 'inter-ligation PETs' were assigned. (d) Clustering of overlapping PET sequences in the same genomic regions to identify enriched protein binding sites by overlapping 'self-ligation PETs' and long-range chromatin interactions by overlapping 'inter-ligation PETs'.

    Article Snippet: Upon Mme I digestion, the resulting PET construct contains a 20 bp head tag, a 38 bp linker sequence, and a 20 bp tail tag, which is the template for next generation paired-end sequencing, for example, Illumina paired-end sequencing from the two ends in opposite directions (Figure ).

    Techniques: ChIA Pet Assay, Chromatin Immunoprecipitation, Ligation, DNA Sequencing, Construct, Sequencing, Positron Emission Tomography, Derivative Assay, Protein Binding