Journal: Cancer cell

Article Title: Therapeutic Targeting of RNA Splicing Catalysis through Inhibition of Protein Arginine Methylation

doi: 10.1016/j.ccell.2019.07.003

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: After lyophilisation, each concatenated fraction was dissolved in 250 μl of 1x immuno-Affinity Purification Buffer (IAP buffer, #9993, Cell Signaling Technologies, CST) and subjected to two consecutive steps of methyl-R-peptides enrichment using the SDMA antibody-conjugated beads (PTMScan [sdme-R] Kit #13563, Cell Signaling Technologies) and the MMA antibody-conjugated beads (PTMScan Mono-Methyl Arginine Motif [mme-RG] Kit #12235, Cell Signaling Technologies), following the manufacturer’s instructions.

Techniques: Recombinant, Luminescence Assay, MTS Assay, Knock-In, Transgenic Assay, Sequencing, Software