mlui  (New England Biolabs)


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  • 99
    Name:
    MluI
    Description:
    MluI 5 000 units
    Catalog Number:
    r0198l
    Price:
    264
    Size:
    5 000 units
    Category:
    Restriction Enzymes
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    Structured Review

    New England Biolabs mlui
    MluI
    MluI 5 000 units
    https://www.bioz.com/result/mlui/product/New England Biolabs
    Average 99 stars, based on 68 article reviews
    Price from $9.99 to $1999.99
    mlui - by Bioz Stars, 2020-01
    99/100 stars

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    Related Articles

    Amplification:

    Article Title: Molecular architecture of G-quadruplex structures generated on duplex Rif1-binding sequences
    Article Snippet: .. The amplified Rif1BSI:2663 fragment was digested by MluI, calf intestinal alkaline phosphatase (New England Biolabs)-treated and end-labeled with 32 P, followed by digestion with BssHII to remove the other end, to obtain 32 P-labeled G-strand. ..

    Purification:

    Article Title: Molecular architecture of G-quadruplex structures generated on duplex Rif1-binding sequences
    Article Snippet: All of the oligonucleotides, OPC column–purified, were further purified by denaturing polyacrylamide gel (containing 8 m urea) if their lengths exceeded 50 nt. .. The amplified Rif1BSI:2663 fragment was digested by MluI, calf intestinal alkaline phosphatase (New England Biolabs)-treated and end-labeled with 32 P, followed by digestion with BssHII to remove the other end, to obtain 32 P-labeled G-strand.

    Incubation:

    Article Title: Molecular architecture of G-quadruplex structures generated on duplex Rif1-binding sequences
    Article Snippet: After incubation at 37 °C for 4 h, the oligonucleotide solution was passed through a Spin-X centrifuge tube filter (Corning), ethanol-precipitated, and resuspended in TE buffer. .. The amplified Rif1BSI:2663 fragment was digested by MluI, calf intestinal alkaline phosphatase (New England Biolabs)-treated and end-labeled with 32 P, followed by digestion with BssHII to remove the other end, to obtain 32 P-labeled G-strand.

    Polyacrylamide Gel Electrophoresis:

    Article Title: Molecular architecture of G-quadruplex structures generated on duplex Rif1-binding sequences
    Article Snippet: Specifically, oligonucleotides, denatured by heating in 95% formamide and 5 m m EDTA, were run on 10% PAGE containing 8 m urea and eluted from the gel by crushing the gel pieces containing the DNA in elution buffer (0.5 m ammonium acetate, 10 m m magnesium acetate, 1 m m EDTA, and 0.1% SDS). .. The amplified Rif1BSI:2663 fragment was digested by MluI, calf intestinal alkaline phosphatase (New England Biolabs)-treated and end-labeled with 32 P, followed by digestion with BssHII to remove the other end, to obtain 32 P-labeled G-strand.

    Polymerase Chain Reaction:

    Article Title: Molecular architecture of G-quadruplex structures generated on duplex Rif1-binding sequences
    Article Snippet: Rif1BS duplex DNAs were PCR-amplified on the fission yeast genomic DNA by using Rif1bs-s-chr1(MluI) (gcgacgcgtCGCATGTACGTATTTCTTTTTACA) + Rif1bs-as-chr1(BssHII) (gttggcgcgcGGGTAAGTCTATTCCGTCTCACTT) for Rif1BSI:2663 and Rif1bs-s-chr2(BssHII) (gttggcgcgcCGCTTTCGGTTTTTATAGAAGCT) + Rif1bs-ss-chr2(MluI) (gcgacgcGTACTCATAATTCCACTATTCCT) for Rif1BSII:4255 . .. The amplified Rif1BSI:2663 fragment was digested by MluI, calf intestinal alkaline phosphatase (New England Biolabs)-treated and end-labeled with 32 P, followed by digestion with BssHII to remove the other end, to obtain 32 P-labeled G-strand.

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  • 90
    New England Biolabs r3198s
    R3198s, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 90/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/r3198s/product/New England Biolabs
    Average 90 stars, based on 10 article reviews
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