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Journal: STAR Protocols

Article Title: Protocol for establishing knockout cell clones by deletion of a large gene fragment using CRISPR-Cas9 with multiple guide RNAs

doi: 10.1016/j.xpro.2024.103179

Figure Lengend Snippet:

Article Snippet: The primers used are listed in the . h. Run 4 μL of the PCR product on a 2% agarose gel. i. Expose the gel with a transilluminator (MaestroGen) and detect the bands.

Techniques: Virus, Recombinant, Modification, Saline, Electron Microscopy, Membrane, Western Blot, Plasmid Preparation, Knock-Out, Software, CRISPR, Blocking Assay, Cell Counting, Electrophoresis, Fluorescence, Microscopy