Journal: Cell Death & Disease

Article Title: Reciprocal regulation of oxidative stress and mitochondrial fission augments parvalbumin downregulation through CDK5-DRP1- and GPx1-NF-κB signaling pathways

doi: 10.1038/s41419-024-07050-5

Figure Lengend Snippet: BSO decreases PV and GPx1 expressions, while it increases CDK5 Y15 and DRP1 S616 phosphorylations accompanied by mitochondrial fragmentation without inducing cleaved caspase-3 (cCasp-3) in PV neurons (PV cell degeneration). Roscovitine (Ros) abrogates these phenomena. Mdivi-1 attenuates BSO-induced PV downregulation, DRP1 S616 phosphorylation, reduced GPx1 expression and mitochondrial fission without affecting CDK5 Y15 phosphorylation. a Representative Western blot images of PV, CDK5 Y15, DRP1 S616 and GPx1 levels in the dentate gyrus. b Quantification of PV, CDK5 Y15, DRP1 S616 and GPx1 levels based on Western blot data ( *,#,$ p < 0.05 vs. vehicle, BSO and BSO + Ros-treated animals, n = 7, respectively). c Representative images of cCasp-3 expression in PV neurons. d Representative images of mitochondria in PV neurons. e Quantification of mitochondrial elongation index and mitochondrial network aggregation in PV neurons ( *,#,$ p < 0.05 vs. vehicle, BSO and BSO + Ros-treated animals, n = 35 cells in 7 rats, respectively).

Article Snippet: Mitochondrial marker (mitochondrial complex IV subunit 1, MTCO1) , Mouse , Abcam (#ab14705) , 1:500 (IH).

Techniques: Expressing, Western Blot

Journal: Cell Death & Disease

Article Title: Reciprocal regulation of oxidative stress and mitochondrial fission augments parvalbumin downregulation through CDK5-DRP1- and GPx1-NF-κB signaling pathways

doi: 10.1038/s41419-024-07050-5

Figure Lengend Snippet: WY14643-induced mitochondrial fission decreases PV and GPx1 expressions without altering CDK5 Y15 phosphorylation in PV neurons. Mdivi-1-induced mitochondrial fusion cannot affect PV, GPx1 and CDK5 Y15 intensities in PV neurons. a Representative images of mitochondria, GPx1 expression and CDK5 Y15 phosphorylation in PV neurons. b Quantification of mitochondrial elongation index and mitochondrial network aggregation in PV neurons ( *,# p < 0.05 vs. vehicle and WY14643-treated animals, n = 35 cells in 7 rats, respectively). c Quantification of GPx1 and CDK5 Y15 fluorescent intensity in PV neurons ( *,# p < 0.05 vs. vehicle and WY14643-treated animals, n = 7, respectively).

Article Snippet: Mitochondrial marker (mitochondrial complex IV subunit 1, MTCO1) , Mouse , Abcam (#ab14705) , 1:500 (IH).

Techniques: Expressing

Journal: Cell Death & Disease

Article Title: Reciprocal regulation of oxidative stress and mitochondrial fission augments parvalbumin downregulation through CDK5-DRP1- and GPx1-NF-κB signaling pathways

doi: 10.1038/s41419-024-07050-5

Figure Lengend Snippet: SE decreases the number of PV neurons in the dentate gyrus, accompanied by mitochondrial fragmentation. SE increases CDK5 Y15 and p65 S536 phosphorylation, while it reduces GPx1 expression. NAC ameliorates these post-SE events. a Representative images of PV neurons, mitochondria, CDK5 Y15 phosphorylation, GPx1 expression and p65 S536 phosphorylation in PV neurons. b Quantification of the number of PV neurons in the dentate gyrus ( *,# p < 0.05 vs. control and vehicle-treated animals, n = 7, respectively). c Quantification of mitochondrial elongation index and mitochondrial network aggregation in PV neurons ( *,# p < 0.05 vs. control and vehicle-treated animals, n = 35 cells in 7 rats, respectively). d Quantification of CDK5 Y15, GPx1 and p65 S536 fluorescent intensity in PV neurons ( *,# p < 0.05 vs. control and vehicle-treated animals, n = 7, respectively).

Article Snippet: Mitochondrial marker (mitochondrial complex IV subunit 1, MTCO1) , Mouse , Abcam (#ab14705) , 1:500 (IH).

Techniques: Expressing, Control

Journal: Cell Death & Disease

Article Title: Reciprocal regulation of oxidative stress and mitochondrial fission augments parvalbumin downregulation through CDK5-DRP1- and GPx1-NF-κB signaling pathways

doi: 10.1038/s41419-024-07050-5

Figure Lengend Snippet: Roscovitine (Ros) attenuates SE-induced PV neuronal degeneration, mitochondrial fragmentation, GPx1 downregulation and p65 S536 phosphorylation in PV neurons. a Representative images of PV neurons, mitochondria, GPx1 expression and CDK5 Y15 phosphorylation in PV neurons. b Quantification of the number of PV neurons in the dentate gyrus ( *,# p < 0.05 vs. control and vehicle-treated animals, n = 7, respectively). c Quantification of mitochondrial elongation index and mitochondrial network aggregation in PV neurons ( *,# p < 0.05 vs. control and vehicle-treated animals, n = 35 cells in 7 rats, respectively). d Quantification of GPx1 and p65 S536 fluorescent intensity in PV neurons ( *,# p < 0.05 vs. control and vehicle-treated animals, n = 7, respectively).

Article Snippet: Mitochondrial marker (mitochondrial complex IV subunit 1, MTCO1) , Mouse , Abcam (#ab14705) , 1:500 (IH).

Techniques: Expressing, Control

Journal: Cell Death & Disease

Article Title: Reciprocal regulation of oxidative stress and mitochondrial fission augments parvalbumin downregulation through CDK5-DRP1- and GPx1-NF-κB signaling pathways

doi: 10.1038/s41419-024-07050-5

Figure Lengend Snippet: Mdivi-1 ameliorates SE-induced PV neuronal degeneration, mitochondrial fragmentation, CDK5 Y15 phosphorylation, GPx1 downregulation and p65 S536 phosphorylation in PV neurons. a Representative images of PV neurons, mitochondria, CDK5 Y15 phosphorylation, GPx1 expression and p65 S536 phosphorylation in PV neurons. b Quantification of the number of PV neurons in the dentate gyrus ( *,# p < 0.05 vs. control and vehicle-treated animals, n = 7, respectively). c Quantification of mitochondrial elongation index and mitochondrial network aggregation in PV neurons ( *,# p < 0.05 vs. control and vehicle-treated animals, n = 35 cells in 7 rats, respectively). d Quantification of CDK5 Y15, GPx1 and p65 S536 fluorescent intensity in PV neurons ( *,# p < 0.05 vs. control and vehicle-treated animals, n = 7, respectively).

Article Snippet: Mitochondrial marker (mitochondrial complex IV subunit 1, MTCO1) , Mouse , Abcam (#ab14705) , 1:500 (IH).

Techniques: Expressing, Control

Journal: Cell Death & Disease

Article Title: Reciprocal regulation of oxidative stress and mitochondrial fission augments parvalbumin downregulation through CDK5-DRP1- and GPx1-NF-κB signaling pathways

doi: 10.1038/s41419-024-07050-5

Figure Lengend Snippet: SN50 mitigates SE-induced PV neuronal degeneration without affecting mitochondrial fragmentation, CDK5 Y15 phosphorylation and GPx1 downregulation in PV neurons. a Representative images of PV neurons, mitochondria, CDK5 Y15 phosphorylation and GPx1 expression in PV neurons. b Quantification of the number of PV neurons in the dentate gyrus ( *,# p < 0.05 vs. control and vehicle-treated animals, n = 7, respectively). c Quantification of mitochondrial elongation index and mitochondrial network aggregation in PV neurons ( *,# p < 0.05 vs. control and vehicle-treated animals, n = 35 cells in 7 rats, respectively). d Quantification of CDK5 Y15 and GPx1 fluorescent intensity in PV neurons ( *,# p < 0.05 vs. control and vehicle-treated animals, n = 7, respectively).

Article Snippet: Mitochondrial marker (mitochondrial complex IV subunit 1, MTCO1) , Mouse , Abcam (#ab14705) , 1:500 (IH).

Techniques: Expressing, Control

Journal: Cell Death & Disease

Article Title: Reciprocal regulation of oxidative stress and mitochondrial fission augments parvalbumin downregulation through CDK5-DRP1- and GPx1-NF-κB signaling pathways

doi: 10.1038/s41419-024-07050-5

Figure Lengend Snippet: Under physiological condition, BSO-induced oxidative stress facilitates CDK5-DRP1-mediated mitochondrial fission in PV neurons, which subsequently diminishes PV and GPx1 expressions. Mitochondrial fission-induced oxidative stress also affectes PV and GPx1 expressions. Following SE, mitochondrial fission contributed to PV downregulation and PV neuronal degeneration through CDK5-DRP1- and GPx1-NF-κB-mediated signaling pathways.

Article Snippet: Mitochondrial marker (mitochondrial complex IV subunit 1, MTCO1) , Mouse , Abcam (#ab14705) , 1:500 (IH).

Techniques:

Journal: bioRxiv

Article Title: ExPOSE: A comprehensive toolkit to perform expansion microscopy in plant protoplast systems

doi: 10.1101/2024.07.12.603300

Figure Lengend Snippet: Lattice SIM maximum intensity projection images of Arabidopsis protoplasts pre- and post-expansion with ExPOSE and labeled via immunofluorescence using ( A ) anti-actin and ( B ) anti-mitochondrial matrix marker (GDC-H: H protein of glycine decarboxylase complex (GDC)) antibodies, chlorophyll autofluorescence (magenta). Control antibody treatment consisted of incubating samples with non-immune rabbit IgG labeled with DyLight TM 594. Intensity of antibody channels were matched and leveled. Scale bar; 20 µm.

Article Snippet: The primary antibodies used in this study were anti-actin rabbit polyclonal (AS13 2640, Agrisera, Vannas, Sweden), and mitochondrial matrix marker anti-GDC-H rabbit polyclonal antibody (AS05 074, Agrisera, Vannas, Sweden) at a dilution of 1:250 in blocking buffer.

Techniques: Labeling, Immunofluorescence, Marker, Control