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MitoSciences mitochondrial atpase
Mitochondrial Atpase, supplied by MitoSciences, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mitochondrial atpase/product/MitoSciences
Average 85 stars, based on 1 article reviews
Price from $9.99 to $1999.99
mitochondrial atpase - by Bioz Stars, 2020-05
85/100 stars

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Article Title: Cardioprotection via preserved mitochondrial structure and function in the mPer2-mutant mouse myocardium
Article Snippet: .. Total mitochondrial OXPHOS immunoblots were obtained using whole LV homogenates probed with an antibody cocktail that recognizes subunits of respiratory complexes I-IV and the mitochondrial ATPase (MitoSciences, Eugene, OR). ..

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  • 91
    MitoSciences mouse anti mitochondrial atp synthase subunit α
    PEX5 remains excessively membrane-associated in pex1-1 pex6-1 . Homogenates (H) prepared from 6-d-old dark-grown WT, pex1-1 , pex6-1 , and pex1-1 pex6-1 seedlings were separated by centrifugation to isolate cytosolic supernatant (S) and an organellar pellet, which was resuspended and recentrifuged to provide a final organellar pellet (P) fraction. Fractions were subjected to immunoblotting with the indicated antibodies. HSC70 is cytosolic, and mitochondrial (mito) <t>ATP</t> <t>synthase</t> <t>subunit</t> α and PEX14 localize in the organelle fraction. The positions of molecular mass markers (in kilodaltons) are indicated on the left.
    Mouse Anti Mitochondrial Atp Synthase Subunit α, supplied by MitoSciences, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti mitochondrial atp synthase subunit α/product/MitoSciences
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse anti mitochondrial atp synthase subunit α - by Bioz Stars, 2020-05
    91/100 stars
      Buy from Supplier

    85
    MitoSciences mouse monoclonal antibody against mitochondrial atpase
    Immunofluorescent colocalization analysis of <t>β1-integrin</t> with mitochondrial <t>ATPase</t> (Complex V). Cells from each of the four lines were formalin fixed and permeabilized on a microscope slide and incubated with monoclonal antibodies raised against mitochondrial ATPase and β1-integrin followed by fluorescent labeled secondary antibody. Expression of Complex V is visualized in red while β1-integrin is in green and co-localization of the two proteins is indicated by yellow color in the merged image. Nuclei are visualized using DAPI (blue) and the gray bars represent 20µm as a reference scale.
    Mouse Monoclonal Antibody Against Mitochondrial Atpase, supplied by MitoSciences, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse monoclonal antibody against mitochondrial atpase/product/MitoSciences
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse monoclonal antibody against mitochondrial atpase - by Bioz Stars, 2020-05
    85/100 stars
      Buy from Supplier

    88
    MitoSciences mitochondrial atp synthase subunit
    Immunofluorescent colocalization analysis of <t>β1-integrin</t> with mitochondrial <t>ATPase</t> (Complex V). Cells from each of the four lines were formalin fixed and permeabilized on a microscope slide and incubated with monoclonal antibodies raised against mitochondrial ATPase and β1-integrin followed by fluorescent labeled secondary antibody. Expression of Complex V is visualized in red while β1-integrin is in green and co-localization of the two proteins is indicated by yellow color in the merged image. Nuclei are visualized using DAPI (blue) and the gray bars represent 20µm as a reference scale.
    Mitochondrial Atp Synthase Subunit, supplied by MitoSciences, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mitochondrial atp synthase subunit/product/MitoSciences
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mitochondrial atp synthase subunit - by Bioz Stars, 2020-05
    88/100 stars
      Buy from Supplier

    Image Search Results


    PEX5 remains excessively membrane-associated in pex1-1 pex6-1 . Homogenates (H) prepared from 6-d-old dark-grown WT, pex1-1 , pex6-1 , and pex1-1 pex6-1 seedlings were separated by centrifugation to isolate cytosolic supernatant (S) and an organellar pellet, which was resuspended and recentrifuged to provide a final organellar pellet (P) fraction. Fractions were subjected to immunoblotting with the indicated antibodies. HSC70 is cytosolic, and mitochondrial (mito) ATP synthase subunit α and PEX14 localize in the organelle fraction. The positions of molecular mass markers (in kilodaltons) are indicated on the left.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: A pex1 missense mutation improves peroxisome function in a subset of Arabidopsis pex6 mutants without restoring PEX5 recycling

    doi: 10.1073/pnas.1721279115

    Figure Lengend Snippet: PEX5 remains excessively membrane-associated in pex1-1 pex6-1 . Homogenates (H) prepared from 6-d-old dark-grown WT, pex1-1 , pex6-1 , and pex1-1 pex6-1 seedlings were separated by centrifugation to isolate cytosolic supernatant (S) and an organellar pellet, which was resuspended and recentrifuged to provide a final organellar pellet (P) fraction. Fractions were subjected to immunoblotting with the indicated antibodies. HSC70 is cytosolic, and mitochondrial (mito) ATP synthase subunit α and PEX14 localize in the organelle fraction. The positions of molecular mass markers (in kilodaltons) are indicated on the left.

    Article Snippet: Primary antibodies were incubated with membranes overnight as follows: rabbit anti-PEX1 (1:200; ref. ), anti-PEX5 (1:100; ref. ), anti-PEX6 (1:800–1,000; ref. ), anti-PEX14 (1:10,000; Agrisera AS08), anti-catalase (1:20,000; ref. ), anti-ICL (1:1,000; ref. ), anti-PMDH2 (1:2,000; ref. ), anti-thiolase (1:2,500–1:5,000; ref. ), mouse anti-mitochondrial ATP synthase subunit α (1:2,000; MitoScience MS507) and anti-HSC70 (1:50,000–1:100,000; SPA-817; StressGen Biotechnologies), and rat anti-HA (1:100–1:500; clone 3F10; Roche).

    Techniques: Centrifugation

    PEX5 is predominantly membrane-associated in pex6 mutants Homogenates (H) prepared from seedlings grown for 1 d in light followed by 5 d darkness were separated by centrifugation to isolate an organellar pellet (P) and cytosolic supernatant (S). Fractions were subjected to immunoblotting with the indicated antibodies. HSC70 and PEX7 are predominantly soluble, whereas PEX14 and the mitochondrial (mito) ATP synthase subunit α localize in the organelle fractions. The graph indicates the mean ratio of PEX5 recovered in the supernatant or pellet compared to the amount of PEX5 in the homogenate for that genotype in three biological replicates including the one shown. Error bars indicate SD.

    Journal: The Plant journal : for cell and molecular biology

    Article Title: Disparate peroxisome-related defects in Arabidopsis pex6 and pex26 mutants link peroxisomal retrotranslocation and oil body utilization

    doi: 10.1111/tpj.13641

    Figure Lengend Snippet: PEX5 is predominantly membrane-associated in pex6 mutants Homogenates (H) prepared from seedlings grown for 1 d in light followed by 5 d darkness were separated by centrifugation to isolate an organellar pellet (P) and cytosolic supernatant (S). Fractions were subjected to immunoblotting with the indicated antibodies. HSC70 and PEX7 are predominantly soluble, whereas PEX14 and the mitochondrial (mito) ATP synthase subunit α localize in the organelle fractions. The graph indicates the mean ratio of PEX5 recovered in the supernatant or pellet compared to the amount of PEX5 in the homogenate for that genotype in three biological replicates including the one shown. Error bars indicate SD.

    Article Snippet: Primary antibodies were as follows: rabbit anti-PEX1 (1:200, ), anti-PEX5 (1:100, ), anti-PEX6 (1:800–1000, ), anti-PEX7 (1:2000, ), anti-PEX10 (1:500, ), anti-PEX14 (1:10,000, Agrisera AS08), anti-thiolase (1:2,500-1:5000, ), anti-PMDH2 (1:2,000, ); mouse anti-mitochondrial ATP synthase subunit α (1:2,000, MitoScience MS507) and anti-HSC70 (1:50,000–1:100,000, SPA-817, StressGen Biotechnologies); and rat anti-HA (1:100–1:500, Roche clone 3F10).

    Techniques: Centrifugation

    Immunofluorescent colocalization analysis of β1-integrin with mitochondrial ATPase (Complex V). Cells from each of the four lines were formalin fixed and permeabilized on a microscope slide and incubated with monoclonal antibodies raised against mitochondrial ATPase and β1-integrin followed by fluorescent labeled secondary antibody. Expression of Complex V is visualized in red while β1-integrin is in green and co-localization of the two proteins is indicated by yellow color in the merged image. Nuclei are visualized using DAPI (blue) and the gray bars represent 20µm as a reference scale.

    Journal: PLoS ONE

    Article Title: Integrated Proteomic and Metabolic Analysis of Breast Cancer Progression

    doi: 10.1371/journal.pone.0076220

    Figure Lengend Snippet: Immunofluorescent colocalization analysis of β1-integrin with mitochondrial ATPase (Complex V). Cells from each of the four lines were formalin fixed and permeabilized on a microscope slide and incubated with monoclonal antibodies raised against mitochondrial ATPase and β1-integrin followed by fluorescent labeled secondary antibody. Expression of Complex V is visualized in red while β1-integrin is in green and co-localization of the two proteins is indicated by yellow color in the merged image. Nuclei are visualized using DAPI (blue) and the gray bars represent 20µm as a reference scale.

    Article Snippet: Slides were then incubated overnight at 4°C with primary rabbit monoclonal antibody directed against β1-integrin or mouse monoclonal antibody against mitochondrial ATPase (Mitosciences).

    Techniques: Microscopy, Incubation, Labeling, Expressing