mirneasy mini kit  (Qiagen)


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    Structured Review

    Qiagen mirneasy mini kit
    Mirneasy Mini Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 967 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mirneasy mini kit/product/Qiagen
    Average 99 stars, based on 967 article reviews
    Price from $9.99 to $1999.99
    mirneasy mini kit - by Bioz Stars, 2020-01
    99/100 stars

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    Related Articles

    Transfection:

    Article Title: 6mer seed toxicity in tumor suppressive microRNAs
    Article Snippet: Cells were lysed 48 h after transfection using Qiazol. .. Total RNA was isolated using the miRNeasy Mini Kit (Qiagen, Cat.No# 74004) following the manufacturer’s instructions.

    Article Title: Induction of DISE in ovarian cancer cells in vivo
    Article Snippet: .. Total RNA was isolated 48 hours after initial transfection using the miRNeasy Mini Kit (Qiagen, Cat.No. .. An on column digestion step using the RNAse-free DNAse Set (Qiagen, Cat.No.

    Article Title: Many si/shRNAs can kill cancer cells by targeting multiple survival genes through an off-target mechanism
    Article Snippet: The transfection mix was removed after 9 hrs and RNA harvested 48 hrs after transfection. .. Total RNA was isolated using the miRNeasy Mini Kit (Qiagen, Cat.No.

    Article Title: Small interfering RNAs based on huntingtin trinucleotide repeats are highly toxic to cancer cells
    Article Snippet: .. The transfection mix was removed after 9 h. Total RNA was isolated 48 h after transfection using the miRNeasy Mini Kit (Qiagen, Cat.No. .. An on‐column digestion step using the RNase‐free DNase Set (Qiagen, Cat.No.

    Amplification:

    Article Title: Intermittent Hypoxia Activates Duration-Dependent Protective and Injurious Mechanisms in Mouse Lung Endothelial Cells
    Article Snippet: After isolating miRNAs using the miRNeasy mini kit (Qiagen, Netherlands; cat.nr. .. 217004), the miRNAs were polyadenylated and amplified using a sequence specific primer in addition to an universal primer for qRT-PCR.

    RNA Sequencing Assay:

    Article Title: 6mer seed toxicity in tumor suppressive microRNAs
    Article Snippet: Paragraph title: RNA-Seq analysis ... Total RNA was isolated using the miRNeasy Mini Kit (Qiagen, Cat.No# 74004) following the manufacturer’s instructions.

    Article Title: Induction of DISE in ovarian cancer cells in vivo
    Article Snippet: Paragraph title: Total RNA isolation and RNA-seq analysis ... Total RNA was isolated 48 hours after initial transfection using the miRNeasy Mini Kit (Qiagen, Cat.No.

    Article Title: Many si/shRNAs can kill cancer cells by targeting multiple survival genes through an off-target mechanism
    Article Snippet: Paragraph title: RNA-Seq analysis ... Total RNA was isolated using the miRNeasy Mini Kit (Qiagen, Cat.No.

    Article Title: Small interfering RNAs based on huntingtin trinucleotide repeats are highly toxic to cancer cells
    Article Snippet: Paragraph title: Total RNA isolation and RNA‐Seq analysis ... The transfection mix was removed after 9 h. Total RNA was isolated 48 h after transfection using the miRNeasy Mini Kit (Qiagen, Cat.No.

    Isolation:

    Article Title: 6mer seed toxicity in tumor suppressive microRNAs
    Article Snippet: .. Total RNA was isolated using the miRNeasy Mini Kit (Qiagen, Cat.No# 74004) following the manufacturer’s instructions. .. An on-column digestion step using the RNAse-free DNAse Set (Qiagen, Cat.No# 79254) was included for all RNA-Seq samples.

    Article Title: Induction of DISE in ovarian cancer cells in vivo
    Article Snippet: .. Total RNA was isolated 48 hours after initial transfection using the miRNeasy Mini Kit (Qiagen, Cat.No. .. An on column digestion step using the RNAse-free DNAse Set (Qiagen, Cat.No.

    Article Title: Many si/shRNAs can kill cancer cells by targeting multiple survival genes through an off-target mechanism
    Article Snippet: .. Total RNA was isolated using the miRNeasy Mini Kit (Qiagen, Cat.No. .. An on-column digestion step using the RNAse-free DNAse Set (Qiagen, Cat.No.

    Article Title: Small interfering RNAs based on huntingtin trinucleotide repeats are highly toxic to cancer cells
    Article Snippet: .. The transfection mix was removed after 9 h. Total RNA was isolated 48 h after transfection using the miRNeasy Mini Kit (Qiagen, Cat.No. .. An on‐column digestion step using the RNase‐free DNase Set (Qiagen, Cat.No.

    Next-Generation Sequencing:

    Article Title: Induction of DISE in ovarian cancer cells in vivo
    Article Snippet: Total RNA was isolated 48 hours after initial transfection using the miRNeasy Mini Kit (Qiagen, Cat.No. .. NGS RNA-SEQ library making and sequencing was performed by the University of Chicago Genomics Facility.

    Article Title: Small interfering RNAs based on huntingtin trinucleotide repeats are highly toxic to cancer cells
    Article Snippet: The transfection mix was removed after 9 h. Total RNA was isolated 48 h after transfection using the miRNeasy Mini Kit (Qiagen, Cat.No. .. NGS RNA‐Seq library construction and sequencing was performed by the University of Chicago Genomics Facility.

    Incubation:

    Article Title: 6mer seed toxicity in tumor suppressive microRNAs
    Article Snippet: Cells were lysed after 80 h drug incubation using Qiazol. .. Total RNA was isolated using the miRNeasy Mini Kit (Qiagen, Cat.No# 74004) following the manufacturer’s instructions.

    Infection:

    Article Title: Many si/shRNAs can kill cancer cells by targeting multiple survival genes through an off-target mechanism
    Article Snippet: In addition, 293T cells were infected with either pLKO-shScr or pLKO-shL1 and RNA was isolated (100 hrs after plating) as described above for the infection with shR6. .. Total RNA was isolated using the miRNeasy Mini Kit (Qiagen, Cat.No.

    Sequencing:

    Article Title: Induction of DISE in ovarian cancer cells in vivo
    Article Snippet: Total RNA was isolated 48 hours after initial transfection using the miRNeasy Mini Kit (Qiagen, Cat.No. .. NGS RNA-SEQ library making and sequencing was performed by the University of Chicago Genomics Facility.

    Article Title: Intermittent Hypoxia Activates Duration-Dependent Protective and Injurious Mechanisms in Mouse Lung Endothelial Cells
    Article Snippet: After isolating miRNAs using the miRNeasy mini kit (Qiagen, Netherlands; cat.nr. .. 217004), the miRNAs were polyadenylated and amplified using a sequence specific primer in addition to an universal primer for qRT-PCR.

    Article Title: Small interfering RNAs based on huntingtin trinucleotide repeats are highly toxic to cancer cells
    Article Snippet: The transfection mix was removed after 9 h. Total RNA was isolated 48 h after transfection using the miRNeasy Mini Kit (Qiagen, Cat.No. .. NGS RNA‐Seq library construction and sequencing was performed by the University of Chicago Genomics Facility.

    Generated:

    Article Title: 6mer seed toxicity in tumor suppressive microRNAs
    Article Snippet: Total RNA was isolated using the miRNeasy Mini Kit (Qiagen, Cat.No# 74004) following the manufacturer’s instructions. .. RNA libraries were generated and sequenced (Genomics Core facility at the University of Chicago).

    Article Title: Many si/shRNAs can kill cancer cells by targeting multiple survival genes through an off-target mechanism
    Article Snippet: Total RNA was isolated using the miRNeasy Mini Kit (Qiagen, Cat.No. .. RNA libraries were generated and sequenced (Genomics Core facility at the University of Chicago).

    Quantitative RT-PCR:

    Article Title: Intermittent Hypoxia Activates Duration-Dependent Protective and Injurious Mechanisms in Mouse Lung Endothelial Cells
    Article Snippet: After isolating miRNAs using the miRNeasy mini kit (Qiagen, Netherlands; cat.nr. .. 217004), the miRNAs were polyadenylated and amplified using a sequence specific primer in addition to an universal primer for qRT-PCR.

    Expressing:

    Article Title: Intermittent Hypoxia Activates Duration-Dependent Protective and Injurious Mechanisms in Mouse Lung Endothelial Cells
    Article Snippet: Paragraph title: Analysis of Expression Levels of miRNAs ... After isolating miRNAs using the miRNeasy mini kit (Qiagen, Netherlands; cat.nr.

    Polymerase Chain Reaction:

    Article Title: Intermittent Hypoxia Activates Duration-Dependent Protective and Injurious Mechanisms in Mouse Lung Endothelial Cells
    Article Snippet: Analysis of Expression Levels of miRNAs For miRNA expression profiling we used the miScript miRNA PCR Array Mouse miFinder (Qiagen, Netherlands; cat.nr. .. After isolating miRNAs using the miRNeasy mini kit (Qiagen, Netherlands; cat.nr.

    Chloramphenicol Acetyltransferase Assay:

    Article Title: 6mer seed toxicity in tumor suppressive microRNAs
    Article Snippet: .. Total RNA was isolated using the miRNeasy Mini Kit (Qiagen, Cat.No# 74004) following the manufacturer’s instructions. .. An on-column digestion step using the RNAse-free DNAse Set (Qiagen, Cat.No# 79254) was included for all RNA-Seq samples.

    Article Title: Induction of DISE in ovarian cancer cells in vivo
    Article Snippet: .. Total RNA was isolated 48 hours after initial transfection using the miRNeasy Mini Kit (Qiagen, Cat.No. .. An on column digestion step using the RNAse-free DNAse Set (Qiagen, Cat.No.

    Article Title: Many si/shRNAs can kill cancer cells by targeting multiple survival genes through an off-target mechanism
    Article Snippet: .. Total RNA was isolated using the miRNeasy Mini Kit (Qiagen, Cat.No. .. An on-column digestion step using the RNAse-free DNAse Set (Qiagen, Cat.No.

    Article Title: Intermittent Hypoxia Activates Duration-Dependent Protective and Injurious Mechanisms in Mouse Lung Endothelial Cells
    Article Snippet: .. After isolating miRNAs using the miRNeasy mini kit (Qiagen, Netherlands; cat.nr. .. 217004), the miRNAs were polyadenylated and amplified using a sequence specific primer in addition to an universal primer for qRT-PCR.

    Article Title: Small interfering RNAs based on huntingtin trinucleotide repeats are highly toxic to cancer cells
    Article Snippet: .. The transfection mix was removed after 9 h. Total RNA was isolated 48 h after transfection using the miRNeasy Mini Kit (Qiagen, Cat.No. .. An on‐column digestion step using the RNase‐free DNase Set (Qiagen, Cat.No.

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  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 90
    Qiagen mirneasy mini kit
    Mirneasy Mini Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 967 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mirneasy mini kit/product/Qiagen
    Average 90 stars, based on 967 article reviews
    Price from $9.99 to $1999.99
    mirneasy mini kit - by Bioz Stars, 2020-01
    90/100 stars
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