Journal: International Journal of Molecular Sciences

Article Title: Downregulation of miR-140-3p Contributes to Upregulation of CD38 Protein in Bronchial Smooth Muscle Cells

doi: 10.3390/ijms21217982

Figure Lengend Snippet: List of differentially expressed microRNAs (miRNAs) in bronchial smooth muscle of antigen-induced airway hyperresponsive (AHR) mice.

Article Snippet: miRNA microarray services were contracted out to Cosmo Bio Co., Ltd. (Tokyo, Japan).

Techniques: Sequencing, Expressing

Journal: International Journal of Molecular Sciences

Article Title: Downregulation of miR-140-3p Contributes to Upregulation of CD38 Protein in Bronchial Smooth Muscle Cells

doi: 10.3390/ijms21217982

Figure Lengend Snippet: Downregulation of miR-140-3p in bronchial smooth muscles of antigen-induced airway hyperresponsive (AHR) mice. The ovalbumin (OA)-immunized animals were repeatedly challenged with aerosolized OA solution, and total RNAs including miRNAs were extracted from the main bronchi 24 h after the last challenge. The miR-140-3p levels were determined by quantitative real-time reverse transcriptase-polymerase chain reaction. The relative expression of miR-140-3p to U6 snRNA was calculated by the 2 − ΔΔCT methods as described in the methods section. Results are presented as mean ± S.E. from six animals, respectively. ** p < 0.01 versus control animal (Cont) by unpaired Student’s t -test.

Article Snippet: miRNA microarray services were contracted out to Cosmo Bio Co., Ltd. (Tokyo, Japan).

Techniques: Muscles, Reverse Transcription, Polymerase Chain Reaction, Expressing, Control

Journal: International Journal of Molecular Sciences

Article Title: Downregulation of miR-140-3p Contributes to Upregulation of CD38 Protein in Bronchial Smooth Muscle Cells

doi: 10.3390/ijms21217982

Figure Lengend Snippet: Upregulation of CD38 protein ( A ) but not mRNA ( B ) in bronchial smooth muscles of antigen-induced airway hyperresponsive (AHR) mice. The ovalbumin (OA)-immunized animals were repeatedly challenged with aerosolized OA solution, and proteins and total RNAs including miRNAs were extracted from the main bronchi 24 h after the last challenge. The protein ( A ) and mRNA ( B ) levels of CD38 were determined by immunoblotting and quantitative real-time reverse transcriptase-polymerase chain reaction, respectively. The relative expressions of CD38 to glyceraldehyde 3-phosphate dehydrogenase (GAPDH; ( A , B )) were calculated as described in the methods section. Results are presented as mean ± S.E. from six animals, respectively. ** p < 0.01 versus control animal (Cont) by unpaired Student’s t -test.

Article Snippet: miRNA microarray services were contracted out to Cosmo Bio Co., Ltd. (Tokyo, Japan).

Techniques: Muscles, Western Blot, Reverse Transcription, Polymerase Chain Reaction, Control

Journal: International Journal of Molecular Sciences

Article Title: Downregulation of miR-140-3p Contributes to Upregulation of CD38 Protein in Bronchial Smooth Muscle Cells

doi: 10.3390/ijms21217982

Figure Lengend Snippet: Effects of interleukin-13 (IL-13) on the expressions of miR-140-3p ( A ) and CD38 mRNA ( B ) in cultured human bronchial smooth muscle cells. Cells were treated with IL-13 (100 ng/mL) or its vehicle (Veh) for 24 h and total RNAs including miRNAs were extracted. The gene expressions were determined by quantitative real-time reverse transcriptase-polymerase chain reaction. The relative gene expressions of miR-140-3p to U6 snRNA ( A ) and CD38 to GAPDH mRNAs ( B ) were calculated by the 2 − ΔΔCT methods as described in the methods section. Results are presented as mean ± S.E. from six independent experiments. ** p < 0.01 versus Veh by unpaired Student’s t -test.

Article Snippet: miRNA microarray services were contracted out to Cosmo Bio Co., Ltd. (Tokyo, Japan).

Techniques: Cell Culture, Reverse Transcription, Polymerase Chain Reaction

Journal: The FASEB Journal

Article Title: Extracellular vesicles extracted from young donor serum attenuate inflammaging via partially rejuvenating aged T-cell immunotolerance

doi: 10.1096/fj.201800059R

Figure Lengend Snippet: Characteristics of serum-extracted EVs. A) Comparison of the sizes of EVs extracted from young murine serum by the ExoQuick reagent pretreated with and without using 0.2-μm filters. B) Morphology of EVs from young murine serum used in this project for rejuvenation of inflammaging, photographed by atomic force microscopy (AFM). C, D) Different miRNA expression profiles in heatmap (C) and quantified summary (D) of EVs from young vs. old murine serum, analyzed by murine miRNA microarray with Mus musculus miRBase version-21 arrays that contained 1900 unique mature miRNA probes (miRNA microarray service via LC Sciences).

Article Snippet: Then, 1–3 μg of total RNAs for each sample were used for customized miRNA microarray service from LC Sciences (Houston, TX, USA).

Techniques: Comparison, Microscopy, Expressing, Microarray