s enteritidis  (ATCC)


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    ATCC s enteritidis
    S Enteritidis, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    s enteritidis  (ATCC)


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    ATCC s enteritidis
    S Enteritidis, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    s enteritidis  (ATCC)


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    ATCC s enteritidis
    S Enteritidis, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    tachyplesin 1 against a baumanii xdr ci 2675  (ATCC)


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    ATCC tachyplesin 1 against a baumanii xdr ci 2675
    Minimum inhibitory concentration (MIC) of peptides against Gram-positive and Gram-negative bacteria.
    Tachyplesin 1 Against A Baumanii Xdr Ci 2675, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Mechanism of Action and Therapeutic Potential of the β-Hairpin Antimicrobial Peptide Capitellacin from the Marine Polychaeta Capitella teleta"

    Article Title: Mechanism of Action and Therapeutic Potential of the β-Hairpin Antimicrobial Peptide Capitellacin from the Marine Polychaeta Capitella teleta

    Journal: Marine Drugs

    doi: 10.3390/md20030167

    Minimum inhibitory concentration (MIC) of peptides against Gram-positive and Gram-negative bacteria.
    Figure Legend Snippet: Minimum inhibitory concentration (MIC) of peptides against Gram-positive and Gram-negative bacteria.

    Techniques Used: Concentration Assay

    ( A ) Hemolytic effect on human red blood cells after 1.5 h exposure of capitellacins and tachyplesin-1; ( B ) the effect of the peptides at different concentrations on the permeability of the cytoplasmic membrane of E. coli ML-35p after 1.5 h (ONPG assay). Data are the mean ± SD of three independent experiments.
    Figure Legend Snippet: ( A ) Hemolytic effect on human red blood cells after 1.5 h exposure of capitellacins and tachyplesin-1; ( B ) the effect of the peptides at different concentrations on the permeability of the cytoplasmic membrane of E. coli ML-35p after 1.5 h (ONPG assay). Data are the mean ± SD of three independent experiments.

    Techniques Used: Permeability

    Induction of E. coli MDR CI 1057 resistance to capitellacin, tachyplesin-1, and polymyxin B in Mueller–Hinton medium containing added NaCl. Initial MIC values were: capitellacin 1 (0.5 µM), tachyplesin-1 (0.125 µM), and polymyxin B (0.125 µM). Bacteria that grew at the highest concentration of AMPs on the final passage (day 21) were passaged a further 3 times on drug-free agar plates before determining the final MIC value (“Post”).
    Figure Legend Snippet: Induction of E. coli MDR CI 1057 resistance to capitellacin, tachyplesin-1, and polymyxin B in Mueller–Hinton medium containing added NaCl. Initial MIC values were: capitellacin 1 (0.5 µM), tachyplesin-1 (0.125 µM), and polymyxin B (0.125 µM). Bacteria that grew at the highest concentration of AMPs on the final passage (day 21) were passaged a further 3 times on drug-free agar plates before determining the final MIC value (“Post”).

    Techniques Used: Concentration Assay

    The peptide-induced changes in conductance after an addition of tachyplesin-1 and capitellacin. Zoomed-in records of single fluctuations of conductance induced by the peptides are presented in the right panel. Each peptide was added to both sides of BLM in the experiment. The applied voltage was 50 mV and the buffer solution contained 0.154 M NaCl and 5 mM HEPES (pH 7.4).
    Figure Legend Snippet: The peptide-induced changes in conductance after an addition of tachyplesin-1 and capitellacin. Zoomed-in records of single fluctuations of conductance induced by the peptides are presented in the right panel. Each peptide was added to both sides of BLM in the experiment. The applied voltage was 50 mV and the buffer solution contained 0.154 M NaCl and 5 mM HEPES (pH 7.4).

    Techniques Used:

    Evaluation of the increase in membrane permeability of E. coli ML-35p caused by peptides at a concentration of 1 µM using chromogenic markers: ( A ) the products of o-nitrophenyl-D-galactoside (ONPG, abs 405 nm) hydrolysis; ( B ) nitrocefin, abs 540 nm. Control measurements were carried out without peptides and showed no penetration of substrates through bacterial membranes. The experiment was carried out twice; the curve behaviors were similar. ( C ) Comparison of hemolytic activity of capitellacin and tachyplesin-1 at a concentration 128 µM against erythrocytes from human after 2 h and 24 h incubation (hemoglobin release assay). ±SD of at least two independent experiments, *** p ≤ 0.001.
    Figure Legend Snippet: Evaluation of the increase in membrane permeability of E. coli ML-35p caused by peptides at a concentration of 1 µM using chromogenic markers: ( A ) the products of o-nitrophenyl-D-galactoside (ONPG, abs 405 nm) hydrolysis; ( B ) nitrocefin, abs 540 nm. Control measurements were carried out without peptides and showed no penetration of substrates through bacterial membranes. The experiment was carried out twice; the curve behaviors were similar. ( C ) Comparison of hemolytic activity of capitellacin and tachyplesin-1 at a concentration 128 µM against erythrocytes from human after 2 h and 24 h incubation (hemoglobin release assay). ±SD of at least two independent experiments, *** p ≤ 0.001.

    Techniques Used: Permeability, Concentration Assay, Activity Assay, Incubation, Release Assay

    Anti-biofilm activity of capitellacin and tachyplesin-1 against E. coli SBS 1936. ( A ) Biofilm formation was established using the crystal violet stain technique. Sterile water was used as solvent for serial dilutions of peptides. Up arrow is MIC of peptides. The ability of capitellacin ( B ) and tachyplesin-1 ( C ) to impact on preformed biofilms of E. coli SBS 1936 after overnight exposure. Evaluation of mass of biofilm was assessed using the crystal violet stain technique. The change in the percentage of surviving bacteria in the biofilm was controlled using MTT assay (statistically significant differences vs. the control: * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001).
    Figure Legend Snippet: Anti-biofilm activity of capitellacin and tachyplesin-1 against E. coli SBS 1936. ( A ) Biofilm formation was established using the crystal violet stain technique. Sterile water was used as solvent for serial dilutions of peptides. Up arrow is MIC of peptides. The ability of capitellacin ( B ) and tachyplesin-1 ( C ) to impact on preformed biofilms of E. coli SBS 1936 after overnight exposure. Evaluation of mass of biofilm was assessed using the crystal violet stain technique. The change in the percentage of surviving bacteria in the biofilm was controlled using MTT assay (statistically significant differences vs. the control: * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001).

    Techniques Used: Activity Assay, Staining, MTT Assay

    pp 2675  (ATCC)


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    ATCC pp 2675
    Pp 2675, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    pp 2675  (ATCC)


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    ATCC pp 2675
    Global analysis of alcohol metabolism in P. putida. (A) Pairwise comparisons of Pearson correlations of fitness data from P. putida KT2440 RB–Tn-Seq libraries grown on alcohols as well as glucose, grouped by overall similarity. The legend at the top left shows the Pearson coefficient, with 1 indicating greater similarity and 0 indicating greater dissimilarity. (B) Heat map showing the fitness scores of all alcohol dehydrogenases annotated in the BioCyc database, as well as cytochrome c <t>PP_2675,</t> when grown on various alcohols and glucose. (C) Operonic diagram of the pqq cluster in P. putida and the corresponding biosynthetic pathway for the PQQ cofactor showing how PQQ cofactors are regenerated by cytochrome c. The heat map shows fitness scores for individual pqq cluster genes when grown on alcohols and glucose.
    Pp 2675, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Fatty Acid and Alcohol Metabolism in Pseudomonas putida : Functional Analysis Using Random Barcode Transposon Sequencing"

    Article Title: Fatty Acid and Alcohol Metabolism in Pseudomonas putida : Functional Analysis Using Random Barcode Transposon Sequencing

    Journal: Applied and Environmental Microbiology

    doi: 10.1128/AEM.01665-20

    Global analysis of alcohol metabolism in P. putida. (A) Pairwise comparisons of Pearson correlations of fitness data from P. putida KT2440 RB–Tn-Seq libraries grown on alcohols as well as glucose, grouped by overall similarity. The legend at the top left shows the Pearson coefficient, with 1 indicating greater similarity and 0 indicating greater dissimilarity. (B) Heat map showing the fitness scores of all alcohol dehydrogenases annotated in the BioCyc database, as well as cytochrome c PP_2675, when grown on various alcohols and glucose. (C) Operonic diagram of the pqq cluster in P. putida and the corresponding biosynthetic pathway for the PQQ cofactor showing how PQQ cofactors are regenerated by cytochrome c. The heat map shows fitness scores for individual pqq cluster genes when grown on alcohols and glucose.
    Figure Legend Snippet: Global analysis of alcohol metabolism in P. putida. (A) Pairwise comparisons of Pearson correlations of fitness data from P. putida KT2440 RB–Tn-Seq libraries grown on alcohols as well as glucose, grouped by overall similarity. The legend at the top left shows the Pearson coefficient, with 1 indicating greater similarity and 0 indicating greater dissimilarity. (B) Heat map showing the fitness scores of all alcohol dehydrogenases annotated in the BioCyc database, as well as cytochrome c PP_2675, when grown on various alcohols and glucose. (C) Operonic diagram of the pqq cluster in P. putida and the corresponding biosynthetic pathway for the PQQ cofactor showing how PQQ cofactors are regenerated by cytochrome c. The heat map shows fitness scores for individual pqq cluster genes when grown on alcohols and glucose.

    Techniques Used:

    Analysis of short-chain-alcohol metabolism in P. putida. (A) Putative genes involved in the initial oxidation steps of short-chain-alcohol assimilation in P. putida. PP_2675 (PedF) is involved in the regeneration of the PQQ cofactor predicted to be necessary for these oxidation reactions of PP_2764 (PedE) and PP_2769 (PedH). Average fitness scores for two biological repetitions are shown next to each gene for ethanol (black), butanol (green), and pentanol (blue). (B to D) Scatterplots showing global fitness scores for ethanol versus acetate (B), butanol versus butyrate (C), and pentanol versus valerate (D).
    Figure Legend Snippet: Analysis of short-chain-alcohol metabolism in P. putida. (A) Putative genes involved in the initial oxidation steps of short-chain-alcohol assimilation in P. putida. PP_2675 (PedF) is involved in the regeneration of the PQQ cofactor predicted to be necessary for these oxidation reactions of PP_2764 (PedE) and PP_2769 (PedH). Average fitness scores for two biological repetitions are shown next to each gene for ethanol (black), butanol (green), and pentanol (blue). (B to D) Scatterplots showing global fitness scores for ethanol versus acetate (B), butanol versus butyrate (C), and pentanol versus valerate (D).

    Techniques Used:

    Putative routes of 1,4-butanediol catabolism in P. putida. Shown are the putative genes involved in catabolism of 1,4-butanediol in P. putida. Average fitness scores for two biological repetitions are shown next to each gene. The three CoA ligases shown were proposed by Li et al. (30); there were no CoA ligases that showed significant fitness defects on 1,4-butanediol. *, PP_2675 (PedF) is involved in the regeneration of the PQQ cofactor predicted to be necessary for these oxidation reactions of PP_2764 (PedE) and PP_2769 (PedH).
    Figure Legend Snippet: Putative routes of 1,4-butanediol catabolism in P. putida. Shown are the putative genes involved in catabolism of 1,4-butanediol in P. putida. Average fitness scores for two biological repetitions are shown next to each gene. The three CoA ligases shown were proposed by Li et al. (30); there were no CoA ligases that showed significant fitness defects on 1,4-butanediol. *, PP_2675 (PedF) is involved in the regeneration of the PQQ cofactor predicted to be necessary for these oxidation reactions of PP_2764 (PedE) and PP_2769 (PedH).

    Techniques Used:

    pp 2675  (ATCC)


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    ATCC pp 2675
    Global analysis of alcohol metabolism in P. putida. (A) Pairwise comparisons of Pearson correlations of fitness data from P. putida KT2440 RB–Tn-Seq libraries grown on alcohols as well as glucose, grouped by overall similarity. The legend at the top left shows the Pearson coefficient, with 1 indicating greater similarity and 0 indicating greater dissimilarity. (B) Heat map showing the fitness scores of all alcohol dehydrogenases annotated in the BioCyc database, as well as cytochrome c <t>PP_2675,</t> when grown on various alcohols and glucose. (C) Operonic diagram of the pqq cluster in P. putida and the corresponding biosynthetic pathway for the PQQ cofactor showing how PQQ cofactors are regenerated by cytochrome c. The heat map shows fitness scores for individual pqq cluster genes when grown on alcohols and glucose.
    Pp 2675, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Fatty Acid and Alcohol Metabolism in Pseudomonas putida : Functional Analysis Using Random Barcode Transposon Sequencing"

    Article Title: Fatty Acid and Alcohol Metabolism in Pseudomonas putida : Functional Analysis Using Random Barcode Transposon Sequencing

    Journal: Applied and Environmental Microbiology

    doi: 10.1128/AEM.01665-20

    Global analysis of alcohol metabolism in P. putida. (A) Pairwise comparisons of Pearson correlations of fitness data from P. putida KT2440 RB–Tn-Seq libraries grown on alcohols as well as glucose, grouped by overall similarity. The legend at the top left shows the Pearson coefficient, with 1 indicating greater similarity and 0 indicating greater dissimilarity. (B) Heat map showing the fitness scores of all alcohol dehydrogenases annotated in the BioCyc database, as well as cytochrome c PP_2675, when grown on various alcohols and glucose. (C) Operonic diagram of the pqq cluster in P. putida and the corresponding biosynthetic pathway for the PQQ cofactor showing how PQQ cofactors are regenerated by cytochrome c. The heat map shows fitness scores for individual pqq cluster genes when grown on alcohols and glucose.
    Figure Legend Snippet: Global analysis of alcohol metabolism in P. putida. (A) Pairwise comparisons of Pearson correlations of fitness data from P. putida KT2440 RB–Tn-Seq libraries grown on alcohols as well as glucose, grouped by overall similarity. The legend at the top left shows the Pearson coefficient, with 1 indicating greater similarity and 0 indicating greater dissimilarity. (B) Heat map showing the fitness scores of all alcohol dehydrogenases annotated in the BioCyc database, as well as cytochrome c PP_2675, when grown on various alcohols and glucose. (C) Operonic diagram of the pqq cluster in P. putida and the corresponding biosynthetic pathway for the PQQ cofactor showing how PQQ cofactors are regenerated by cytochrome c. The heat map shows fitness scores for individual pqq cluster genes when grown on alcohols and glucose.

    Techniques Used:

    Analysis of short-chain-alcohol metabolism in P. putida. (A) Putative genes involved in the initial oxidation steps of short-chain-alcohol assimilation in P. putida. PP_2675 (PedF) is involved in the regeneration of the PQQ cofactor predicted to be necessary for these oxidation reactions of PP_2764 (PedE) and PP_2769 (PedH). Average fitness scores for two biological repetitions are shown next to each gene for ethanol (black), butanol (green), and pentanol (blue). (B to D) Scatterplots showing global fitness scores for ethanol versus acetate (B), butanol versus butyrate (C), and pentanol versus valerate (D).
    Figure Legend Snippet: Analysis of short-chain-alcohol metabolism in P. putida. (A) Putative genes involved in the initial oxidation steps of short-chain-alcohol assimilation in P. putida. PP_2675 (PedF) is involved in the regeneration of the PQQ cofactor predicted to be necessary for these oxidation reactions of PP_2764 (PedE) and PP_2769 (PedH). Average fitness scores for two biological repetitions are shown next to each gene for ethanol (black), butanol (green), and pentanol (blue). (B to D) Scatterplots showing global fitness scores for ethanol versus acetate (B), butanol versus butyrate (C), and pentanol versus valerate (D).

    Techniques Used:

    Putative routes of 1,4-butanediol catabolism in P. putida. Shown are the putative genes involved in catabolism of 1,4-butanediol in P. putida. Average fitness scores for two biological repetitions are shown next to each gene. The three CoA ligases shown were proposed by Li et al. (30); there were no CoA ligases that showed significant fitness defects on 1,4-butanediol. *, PP_2675 (PedF) is involved in the regeneration of the PQQ cofactor predicted to be necessary for these oxidation reactions of PP_2764 (PedE) and PP_2769 (PedH).
    Figure Legend Snippet: Putative routes of 1,4-butanediol catabolism in P. putida. Shown are the putative genes involved in catabolism of 1,4-butanediol in P. putida. Average fitness scores for two biological repetitions are shown next to each gene. The three CoA ligases shown were proposed by Li et al. (30); there were no CoA ligases that showed significant fitness defects on 1,4-butanediol. *, PP_2675 (PedF) is involved in the regeneration of the PQQ cofactor predicted to be necessary for these oxidation reactions of PP_2764 (PedE) and PP_2769 (PedH).

    Techniques Used:

    pp 2675  (ATCC)


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    ATCC pp 2675
    Pp 2675, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    pp 2675  (ATCC)


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    ATCC pp 2675
    Global analysis of alcohol metabolism in P. putida. (A) Pairwise comparisons of Pearson correlations of fitness data from P. putida KT2440 RB–Tn-Seq libraries grown on alcohols as well as glucose, grouped by overall similarity. The legend at the top left shows the Pearson coefficient, with 1 indicating greater similarity and 0 indicating greater dissimilarity. (B) Heat map showing the fitness scores of all alcohol dehydrogenases annotated in the BioCyc database, as well as cytochrome c <t>PP_2675,</t> when grown on various alcohols and glucose. (C) Operonic diagram of the pqq cluster in P. putida and the corresponding biosynthetic pathway for the PQQ cofactor showing how PQQ cofactors are regenerated by cytochrome c. The heat map shows fitness scores for individual pqq cluster genes when grown on alcohols and glucose.
    Pp 2675, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pp 2675/product/ATCC
    Average 94 stars, based on 1 article reviews
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    pp 2675 - by Bioz Stars, 2023-02
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    Images

    1) Product Images from "Fatty Acid and Alcohol Metabolism in Pseudomonas putida : Functional Analysis Using Random Barcode Transposon Sequencing"

    Article Title: Fatty Acid and Alcohol Metabolism in Pseudomonas putida : Functional Analysis Using Random Barcode Transposon Sequencing

    Journal: Applied and Environmental Microbiology

    doi: 10.1128/AEM.01665-20

    Global analysis of alcohol metabolism in P. putida. (A) Pairwise comparisons of Pearson correlations of fitness data from P. putida KT2440 RB–Tn-Seq libraries grown on alcohols as well as glucose, grouped by overall similarity. The legend at the top left shows the Pearson coefficient, with 1 indicating greater similarity and 0 indicating greater dissimilarity. (B) Heat map showing the fitness scores of all alcohol dehydrogenases annotated in the BioCyc database, as well as cytochrome c PP_2675, when grown on various alcohols and glucose. (C) Operonic diagram of the pqq cluster in P. putida and the corresponding biosynthetic pathway for the PQQ cofactor showing how PQQ cofactors are regenerated by cytochrome c. The heat map shows fitness scores for individual pqq cluster genes when grown on alcohols and glucose.
    Figure Legend Snippet: Global analysis of alcohol metabolism in P. putida. (A) Pairwise comparisons of Pearson correlations of fitness data from P. putida KT2440 RB–Tn-Seq libraries grown on alcohols as well as glucose, grouped by overall similarity. The legend at the top left shows the Pearson coefficient, with 1 indicating greater similarity and 0 indicating greater dissimilarity. (B) Heat map showing the fitness scores of all alcohol dehydrogenases annotated in the BioCyc database, as well as cytochrome c PP_2675, when grown on various alcohols and glucose. (C) Operonic diagram of the pqq cluster in P. putida and the corresponding biosynthetic pathway for the PQQ cofactor showing how PQQ cofactors are regenerated by cytochrome c. The heat map shows fitness scores for individual pqq cluster genes when grown on alcohols and glucose.

    Techniques Used:

    Analysis of short-chain-alcohol metabolism in P. putida. (A) Putative genes involved in the initial oxidation steps of short-chain-alcohol assimilation in P. putida. PP_2675 (PedF) is involved in the regeneration of the PQQ cofactor predicted to be necessary for these oxidation reactions of PP_2764 (PedE) and PP_2769 (PedH). Average fitness scores for two biological repetitions are shown next to each gene for ethanol (black), butanol (green), and pentanol (blue). (B to D) Scatterplots showing global fitness scores for ethanol versus acetate (B), butanol versus butyrate (C), and pentanol versus valerate (D).
    Figure Legend Snippet: Analysis of short-chain-alcohol metabolism in P. putida. (A) Putative genes involved in the initial oxidation steps of short-chain-alcohol assimilation in P. putida. PP_2675 (PedF) is involved in the regeneration of the PQQ cofactor predicted to be necessary for these oxidation reactions of PP_2764 (PedE) and PP_2769 (PedH). Average fitness scores for two biological repetitions are shown next to each gene for ethanol (black), butanol (green), and pentanol (blue). (B to D) Scatterplots showing global fitness scores for ethanol versus acetate (B), butanol versus butyrate (C), and pentanol versus valerate (D).

    Techniques Used:

    Putative routes of 1,4-butanediol catabolism in P. putida. Shown are the putative genes involved in catabolism of 1,4-butanediol in P. putida. Average fitness scores for two biological repetitions are shown next to each gene. The three CoA ligases shown were proposed by Li et al. (30); there were no CoA ligases that showed significant fitness defects on 1,4-butanediol. *, PP_2675 (PedF) is involved in the regeneration of the PQQ cofactor predicted to be necessary for these oxidation reactions of PP_2764 (PedE) and PP_2769 (PedH).
    Figure Legend Snippet: Putative routes of 1,4-butanediol catabolism in P. putida. Shown are the putative genes involved in catabolism of 1,4-butanediol in P. putida. Average fitness scores for two biological repetitions are shown next to each gene. The three CoA ligases shown were proposed by Li et al. (30); there were no CoA ligases that showed significant fitness defects on 1,4-butanediol. *, PP_2675 (PedF) is involved in the regeneration of the PQQ cofactor predicted to be necessary for these oxidation reactions of PP_2764 (PedE) and PP_2769 (PedH).

    Techniques Used:

    uterine lavage  (ATCC)


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    ATCC uterine lavage
    Uterine Lavage, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    mink neovison vison  (ATCC)


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    ATCC mink neovison vison
    Mink Neovison Vison, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC s enteritidis
    S Enteritidis, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC tachyplesin 1 against a baumanii xdr ci 2675
    Minimum inhibitory concentration (MIC) of peptides against Gram-positive and Gram-negative bacteria.
    Tachyplesin 1 Against A Baumanii Xdr Ci 2675, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC pp 2675
    Minimum inhibitory concentration (MIC) of peptides against Gram-positive and Gram-negative bacteria.
    Pp 2675, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC uterine lavage
    Minimum inhibitory concentration (MIC) of peptides against Gram-positive and Gram-negative bacteria.
    Uterine Lavage, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC mink neovison vison
    Minimum inhibitory concentration (MIC) of peptides against Gram-positive and Gram-negative bacteria.
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    Image Search Results


    Minimum inhibitory concentration (MIC) of peptides against Gram-positive and Gram-negative bacteria.

    Journal: Marine Drugs

    Article Title: Mechanism of Action and Therapeutic Potential of the β-Hairpin Antimicrobial Peptide Capitellacin from the Marine Polychaeta Capitella teleta

    doi: 10.3390/md20030167

    Figure Lengend Snippet: Minimum inhibitory concentration (MIC) of peptides against Gram-positive and Gram-negative bacteria.

    Article Snippet: The following supporting information can be downloaded at: https://www.mdpi.com/article/10.3390/md20030167/s1 , Figure S1: Reverse-phase high-performance liquid chromatography purification of peptides; Figure S2: Repurification of peptides was performed using the analytical column; Figure S3: Anti-biofilm activity of capitellacin and tachyplesin-1 against A. baumanii XDR CI 2675, K. pneumonia ATCC 700603 and P. aeruginosa XDR CI 1995.

    Techniques: Concentration Assay

    ( A ) Hemolytic effect on human red blood cells after 1.5 h exposure of capitellacins and tachyplesin-1; ( B ) the effect of the peptides at different concentrations on the permeability of the cytoplasmic membrane of E. coli ML-35p after 1.5 h (ONPG assay). Data are the mean ± SD of three independent experiments.

    Journal: Marine Drugs

    Article Title: Mechanism of Action and Therapeutic Potential of the β-Hairpin Antimicrobial Peptide Capitellacin from the Marine Polychaeta Capitella teleta

    doi: 10.3390/md20030167

    Figure Lengend Snippet: ( A ) Hemolytic effect on human red blood cells after 1.5 h exposure of capitellacins and tachyplesin-1; ( B ) the effect of the peptides at different concentrations on the permeability of the cytoplasmic membrane of E. coli ML-35p after 1.5 h (ONPG assay). Data are the mean ± SD of three independent experiments.

    Article Snippet: The following supporting information can be downloaded at: https://www.mdpi.com/article/10.3390/md20030167/s1 , Figure S1: Reverse-phase high-performance liquid chromatography purification of peptides; Figure S2: Repurification of peptides was performed using the analytical column; Figure S3: Anti-biofilm activity of capitellacin and tachyplesin-1 against A. baumanii XDR CI 2675, K. pneumonia ATCC 700603 and P. aeruginosa XDR CI 1995.

    Techniques: Permeability

    Induction of E. coli MDR CI 1057 resistance to capitellacin, tachyplesin-1, and polymyxin B in Mueller–Hinton medium containing added NaCl. Initial MIC values were: capitellacin 1 (0.5 µM), tachyplesin-1 (0.125 µM), and polymyxin B (0.125 µM). Bacteria that grew at the highest concentration of AMPs on the final passage (day 21) were passaged a further 3 times on drug-free agar plates before determining the final MIC value (“Post”).

    Journal: Marine Drugs

    Article Title: Mechanism of Action and Therapeutic Potential of the β-Hairpin Antimicrobial Peptide Capitellacin from the Marine Polychaeta Capitella teleta

    doi: 10.3390/md20030167

    Figure Lengend Snippet: Induction of E. coli MDR CI 1057 resistance to capitellacin, tachyplesin-1, and polymyxin B in Mueller–Hinton medium containing added NaCl. Initial MIC values were: capitellacin 1 (0.5 µM), tachyplesin-1 (0.125 µM), and polymyxin B (0.125 µM). Bacteria that grew at the highest concentration of AMPs on the final passage (day 21) were passaged a further 3 times on drug-free agar plates before determining the final MIC value (“Post”).

    Article Snippet: The following supporting information can be downloaded at: https://www.mdpi.com/article/10.3390/md20030167/s1 , Figure S1: Reverse-phase high-performance liquid chromatography purification of peptides; Figure S2: Repurification of peptides was performed using the analytical column; Figure S3: Anti-biofilm activity of capitellacin and tachyplesin-1 against A. baumanii XDR CI 2675, K. pneumonia ATCC 700603 and P. aeruginosa XDR CI 1995.

    Techniques: Concentration Assay

    The peptide-induced changes in conductance after an addition of tachyplesin-1 and capitellacin. Zoomed-in records of single fluctuations of conductance induced by the peptides are presented in the right panel. Each peptide was added to both sides of BLM in the experiment. The applied voltage was 50 mV and the buffer solution contained 0.154 M NaCl and 5 mM HEPES (pH 7.4).

    Journal: Marine Drugs

    Article Title: Mechanism of Action and Therapeutic Potential of the β-Hairpin Antimicrobial Peptide Capitellacin from the Marine Polychaeta Capitella teleta

    doi: 10.3390/md20030167

    Figure Lengend Snippet: The peptide-induced changes in conductance after an addition of tachyplesin-1 and capitellacin. Zoomed-in records of single fluctuations of conductance induced by the peptides are presented in the right panel. Each peptide was added to both sides of BLM in the experiment. The applied voltage was 50 mV and the buffer solution contained 0.154 M NaCl and 5 mM HEPES (pH 7.4).

    Article Snippet: The following supporting information can be downloaded at: https://www.mdpi.com/article/10.3390/md20030167/s1 , Figure S1: Reverse-phase high-performance liquid chromatography purification of peptides; Figure S2: Repurification of peptides was performed using the analytical column; Figure S3: Anti-biofilm activity of capitellacin and tachyplesin-1 against A. baumanii XDR CI 2675, K. pneumonia ATCC 700603 and P. aeruginosa XDR CI 1995.

    Techniques:

    Evaluation of the increase in membrane permeability of E. coli ML-35p caused by peptides at a concentration of 1 µM using chromogenic markers: ( A ) the products of o-nitrophenyl-D-galactoside (ONPG, abs 405 nm) hydrolysis; ( B ) nitrocefin, abs 540 nm. Control measurements were carried out without peptides and showed no penetration of substrates through bacterial membranes. The experiment was carried out twice; the curve behaviors were similar. ( C ) Comparison of hemolytic activity of capitellacin and tachyplesin-1 at a concentration 128 µM against erythrocytes from human after 2 h and 24 h incubation (hemoglobin release assay). ±SD of at least two independent experiments, *** p ≤ 0.001.

    Journal: Marine Drugs

    Article Title: Mechanism of Action and Therapeutic Potential of the β-Hairpin Antimicrobial Peptide Capitellacin from the Marine Polychaeta Capitella teleta

    doi: 10.3390/md20030167

    Figure Lengend Snippet: Evaluation of the increase in membrane permeability of E. coli ML-35p caused by peptides at a concentration of 1 µM using chromogenic markers: ( A ) the products of o-nitrophenyl-D-galactoside (ONPG, abs 405 nm) hydrolysis; ( B ) nitrocefin, abs 540 nm. Control measurements were carried out without peptides and showed no penetration of substrates through bacterial membranes. The experiment was carried out twice; the curve behaviors were similar. ( C ) Comparison of hemolytic activity of capitellacin and tachyplesin-1 at a concentration 128 µM against erythrocytes from human after 2 h and 24 h incubation (hemoglobin release assay). ±SD of at least two independent experiments, *** p ≤ 0.001.

    Article Snippet: The following supporting information can be downloaded at: https://www.mdpi.com/article/10.3390/md20030167/s1 , Figure S1: Reverse-phase high-performance liquid chromatography purification of peptides; Figure S2: Repurification of peptides was performed using the analytical column; Figure S3: Anti-biofilm activity of capitellacin and tachyplesin-1 against A. baumanii XDR CI 2675, K. pneumonia ATCC 700603 and P. aeruginosa XDR CI 1995.

    Techniques: Permeability, Concentration Assay, Activity Assay, Incubation, Release Assay

    Anti-biofilm activity of capitellacin and tachyplesin-1 against E. coli SBS 1936. ( A ) Biofilm formation was established using the crystal violet stain technique. Sterile water was used as solvent for serial dilutions of peptides. Up arrow is MIC of peptides. The ability of capitellacin ( B ) and tachyplesin-1 ( C ) to impact on preformed biofilms of E. coli SBS 1936 after overnight exposure. Evaluation of mass of biofilm was assessed using the crystal violet stain technique. The change in the percentage of surviving bacteria in the biofilm was controlled using MTT assay (statistically significant differences vs. the control: * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001).

    Journal: Marine Drugs

    Article Title: Mechanism of Action and Therapeutic Potential of the β-Hairpin Antimicrobial Peptide Capitellacin from the Marine Polychaeta Capitella teleta

    doi: 10.3390/md20030167

    Figure Lengend Snippet: Anti-biofilm activity of capitellacin and tachyplesin-1 against E. coli SBS 1936. ( A ) Biofilm formation was established using the crystal violet stain technique. Sterile water was used as solvent for serial dilutions of peptides. Up arrow is MIC of peptides. The ability of capitellacin ( B ) and tachyplesin-1 ( C ) to impact on preformed biofilms of E. coli SBS 1936 after overnight exposure. Evaluation of mass of biofilm was assessed using the crystal violet stain technique. The change in the percentage of surviving bacteria in the biofilm was controlled using MTT assay (statistically significant differences vs. the control: * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001).

    Article Snippet: The following supporting information can be downloaded at: https://www.mdpi.com/article/10.3390/md20030167/s1 , Figure S1: Reverse-phase high-performance liquid chromatography purification of peptides; Figure S2: Repurification of peptides was performed using the analytical column; Figure S3: Anti-biofilm activity of capitellacin and tachyplesin-1 against A. baumanii XDR CI 2675, K. pneumonia ATCC 700603 and P. aeruginosa XDR CI 1995.

    Techniques: Activity Assay, Staining, MTT Assay