mini protease inhibitor cocktail roche 11836153001  (Roche)

 
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    Structured Review

    Roche mini protease inhibitor cocktail roche 11836153001
    Mini Protease Inhibitor Cocktail Roche 11836153001, supplied by Roche, used in various techniques. Bioz Stars score: 83/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mini protease inhibitor cocktail roche 11836153001/product/Roche
    Average 83 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mini protease inhibitor cocktail roche 11836153001 - by Bioz Stars, 2020-03
    83/100 stars

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    Related Articles

    Transduction:

    Article Title: In trans paired nicking triggers seamless genome editing without double-stranded DNA cutting
    Article Snippet: Transduction experiments were carried out in duplicate to generate parallel samples for genotyping and western blot analysis. .. The lysis buffer was supplemented with the protease inhibitors present in the cOmpleteTM , Mini Protease Inhibitor Cocktail Roche-11836153001 (Sigma-Aldrich).

    Lysis:

    Article Title: In trans paired nicking triggers seamless genome editing without double-stranded DNA cutting
    Article Snippet: .. The lysis buffer was supplemented with the protease inhibitors present in the cOmpleteTM , Mini Protease Inhibitor Cocktail Roche-11836153001 (Sigma-Aldrich). .. Protein concentrations were determined by using the BCA protein assay kit (ThermoFisher Scientific) and a Precisely 1420 Multilabel Plate Counter (PerkinElmer) with the absorption wavelength set at λ = 545 nm.

    Western Blot:

    Article Title: In trans paired nicking triggers seamless genome editing without double-stranded DNA cutting
    Article Snippet: Likewise, at 3 days post-transduction, protein lysates were prepared under ice-cold conditions for western blot analysis as follows. .. The lysis buffer was supplemented with the protease inhibitors present in the cOmpleteTM , Mini Protease Inhibitor Cocktail Roche-11836153001 (Sigma-Aldrich).

    Protease Inhibitor:

    Article Title: In trans paired nicking triggers seamless genome editing without double-stranded DNA cutting
    Article Snippet: .. The lysis buffer was supplemented with the protease inhibitors present in the cOmpleteTM , Mini Protease Inhibitor Cocktail Roche-11836153001 (Sigma-Aldrich). .. Protein concentrations were determined by using the BCA protein assay kit (ThermoFisher Scientific) and a Precisely 1420 Multilabel Plate Counter (PerkinElmer) with the absorption wavelength set at λ = 545 nm.

    BIA-KA:

    Article Title: In trans paired nicking triggers seamless genome editing without double-stranded DNA cutting
    Article Snippet: The lysis buffer was supplemented with the protease inhibitors present in the cOmpleteTM , Mini Protease Inhibitor Cocktail Roche-11836153001 (Sigma-Aldrich). .. Protein concentrations were determined by using the BCA protein assay kit (ThermoFisher Scientific) and a Precisely 1420 Multilabel Plate Counter (PerkinElmer) with the absorption wavelength set at λ = 545 nm.

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  • 99
    Roche complete mini protease inhibitor cocktail
    CRISPR/Cas9 mutagenesis of lamc3 causes defects in parachordal chain (PAC) development. ( A ) Design of sgRNAs targeted to exon1 of the lamc3 gene. Each sgRNA targets a restriction endonuclease site used for genotyping and is upstream of a protospacer adjustment motif (PAM). ( B – D ) Cas9/sgRNA injected embryos (as labelled) at 50 hpf. ( B – D ) Brightfield images show no developmental defects or developmental delay. ( B′ – D′ ) Whole embryo images of Tg( fli1a :egfp) fluorescence area indicated by white dotted line is enlarged in ( B′′ – D′′ ) the PAC in each hemisegment is indicated with a red asterisk. ( E ) Quantification of Tg( fli1a :egfp) embryos at 50 hpf with PAC defects. P-values determined by Fisher’s exact Two-tailed test compared to Cas9 alone controls. ( F – I ) 2% agarose genotyping gels with 100 bp ladder. ( F ) Schematic diagram of the region amplified by PCR contains two SphI sites (orange), which would digest into three fragments of 83, 164 and 263 bp in length. Predicted mutation of one of these sites will produce two bands of 83 and 427 bp in length. A natural variant contains a SNP in the second SphI site, <t>complete</t> digestion of this variant would produce two bands of 164 and 346 bp in length. Mutation of the target SphI site would prevent digestion of the fragment. Digestion of the amplicon with Taq α I (blue) in wild type embryos will yield two fragments of 204 and 306 bp in size. ( G ) Restriction endonuclease digest of target region shows undigested mutant products (black asterisk) at a size of 427/510 bp in sgRNA1-injected embryos. ( H ) Fewer undigested mutant products (510 bp) were observed in sgRNA2-injected embryos. ( I ) Brightfield images sgRNA1/Cas9 injected embryo at 5 dpf shows severe oedema. ( J ) Genotyping of 3-month adult fish. A, anterior; b/bp, base pairs; d, days post fertilisation; D, dorsal; kb, kilobases; mpf, months post fertilisation; n, number of embryos; PAC, parachordal chain; PAM, protospacer adjustment motif; SBMO, splice-blocking morpholino; sgRNA, short guide RNA; U, undigested control;.
    Complete Mini Protease Inhibitor Cocktail, supplied by Roche, used in various techniques. Bioz Stars score: 99/100, based on 350 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/complete mini protease inhibitor cocktail/product/Roche
    Average 99 stars, based on 350 article reviews
    Price from $9.99 to $1999.99
    complete mini protease inhibitor cocktail - by Bioz Stars, 2020-03
    99/100 stars
      Buy from Supplier

    99
    Roche protease inhibitor cocktail
    CRISPR/Cas9 mutagenesis of lamc3 causes defects in parachordal chain (PAC) development. ( A ) Design of sgRNAs targeted to exon1 of the lamc3 gene. Each sgRNA targets a restriction endonuclease site used for genotyping and is upstream of a protospacer adjustment motif (PAM). ( B – D ) Cas9/sgRNA injected embryos (as labelled) at 50 hpf. ( B – D ) Brightfield images show no developmental defects or developmental delay. ( B′ – D′ ) Whole embryo images of Tg( fli1a :egfp) fluorescence area indicated by white dotted line is enlarged in ( B′′ – D′′ ) the PAC in each hemisegment is indicated with a red asterisk. ( E ) Quantification of Tg( fli1a :egfp) embryos at 50 hpf with PAC defects. P-values determined by Fisher’s exact Two-tailed test compared to Cas9 alone controls. ( F – I ) 2% agarose genotyping gels with 100 bp ladder. ( F ) Schematic diagram of the region amplified by PCR contains two SphI sites (orange), which would digest into three fragments of 83, 164 and 263 bp in length. Predicted mutation of one of these sites will produce two bands of 83 and 427 bp in length. A natural variant contains a SNP in the second SphI site, <t>complete</t> digestion of this variant would produce two bands of 164 and 346 bp in length. Mutation of the target SphI site would prevent digestion of the fragment. Digestion of the amplicon with Taq α I (blue) in wild type embryos will yield two fragments of 204 and 306 bp in size. ( G ) Restriction endonuclease digest of target region shows undigested mutant products (black asterisk) at a size of 427/510 bp in sgRNA1-injected embryos. ( H ) Fewer undigested mutant products (510 bp) were observed in sgRNA2-injected embryos. ( I ) Brightfield images sgRNA1/Cas9 injected embryo at 5 dpf shows severe oedema. ( J ) Genotyping of 3-month adult fish. A, anterior; b/bp, base pairs; d, days post fertilisation; D, dorsal; kb, kilobases; mpf, months post fertilisation; n, number of embryos; PAC, parachordal chain; PAM, protospacer adjustment motif; SBMO, splice-blocking morpholino; sgRNA, short guide RNA; U, undigested control;.
    Protease Inhibitor Cocktail, supplied by Roche, used in various techniques. Bioz Stars score: 99/100, based on 8944 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/protease inhibitor cocktail/product/Roche
    Average 99 stars, based on 8944 article reviews
    Price from $9.99 to $1999.99
    protease inhibitor cocktail - by Bioz Stars, 2020-03
    99/100 stars
      Buy from Supplier

    Image Search Results


    CRISPR/Cas9 mutagenesis of lamc3 causes defects in parachordal chain (PAC) development. ( A ) Design of sgRNAs targeted to exon1 of the lamc3 gene. Each sgRNA targets a restriction endonuclease site used for genotyping and is upstream of a protospacer adjustment motif (PAM). ( B – D ) Cas9/sgRNA injected embryos (as labelled) at 50 hpf. ( B – D ) Brightfield images show no developmental defects or developmental delay. ( B′ – D′ ) Whole embryo images of Tg( fli1a :egfp) fluorescence area indicated by white dotted line is enlarged in ( B′′ – D′′ ) the PAC in each hemisegment is indicated with a red asterisk. ( E ) Quantification of Tg( fli1a :egfp) embryos at 50 hpf with PAC defects. P-values determined by Fisher’s exact Two-tailed test compared to Cas9 alone controls. ( F – I ) 2% agarose genotyping gels with 100 bp ladder. ( F ) Schematic diagram of the region amplified by PCR contains two SphI sites (orange), which would digest into three fragments of 83, 164 and 263 bp in length. Predicted mutation of one of these sites will produce two bands of 83 and 427 bp in length. A natural variant contains a SNP in the second SphI site, complete digestion of this variant would produce two bands of 164 and 346 bp in length. Mutation of the target SphI site would prevent digestion of the fragment. Digestion of the amplicon with Taq α I (blue) in wild type embryos will yield two fragments of 204 and 306 bp in size. ( G ) Restriction endonuclease digest of target region shows undigested mutant products (black asterisk) at a size of 427/510 bp in sgRNA1-injected embryos. ( H ) Fewer undigested mutant products (510 bp) were observed in sgRNA2-injected embryos. ( I ) Brightfield images sgRNA1/Cas9 injected embryo at 5 dpf shows severe oedema. ( J ) Genotyping of 3-month adult fish. A, anterior; b/bp, base pairs; d, days post fertilisation; D, dorsal; kb, kilobases; mpf, months post fertilisation; n, number of embryos; PAC, parachordal chain; PAM, protospacer adjustment motif; SBMO, splice-blocking morpholino; sgRNA, short guide RNA; U, undigested control;.

    Journal: Wellcome Open Research

    Article Title: Knockdown of Laminin gamma-3 (Lamc3) impairs motoneuron guidance in the zebrafish embryo

    doi: 10.12688/wellcomeopenres.12394.1

    Figure Lengend Snippet: CRISPR/Cas9 mutagenesis of lamc3 causes defects in parachordal chain (PAC) development. ( A ) Design of sgRNAs targeted to exon1 of the lamc3 gene. Each sgRNA targets a restriction endonuclease site used for genotyping and is upstream of a protospacer adjustment motif (PAM). ( B – D ) Cas9/sgRNA injected embryos (as labelled) at 50 hpf. ( B – D ) Brightfield images show no developmental defects or developmental delay. ( B′ – D′ ) Whole embryo images of Tg( fli1a :egfp) fluorescence area indicated by white dotted line is enlarged in ( B′′ – D′′ ) the PAC in each hemisegment is indicated with a red asterisk. ( E ) Quantification of Tg( fli1a :egfp) embryos at 50 hpf with PAC defects. P-values determined by Fisher’s exact Two-tailed test compared to Cas9 alone controls. ( F – I ) 2% agarose genotyping gels with 100 bp ladder. ( F ) Schematic diagram of the region amplified by PCR contains two SphI sites (orange), which would digest into three fragments of 83, 164 and 263 bp in length. Predicted mutation of one of these sites will produce two bands of 83 and 427 bp in length. A natural variant contains a SNP in the second SphI site, complete digestion of this variant would produce two bands of 164 and 346 bp in length. Mutation of the target SphI site would prevent digestion of the fragment. Digestion of the amplicon with Taq α I (blue) in wild type embryos will yield two fragments of 204 and 306 bp in size. ( G ) Restriction endonuclease digest of target region shows undigested mutant products (black asterisk) at a size of 427/510 bp in sgRNA1-injected embryos. ( H ) Fewer undigested mutant products (510 bp) were observed in sgRNA2-injected embryos. ( I ) Brightfield images sgRNA1/Cas9 injected embryo at 5 dpf shows severe oedema. ( J ) Genotyping of 3-month adult fish. A, anterior; b/bp, base pairs; d, days post fertilisation; D, dorsal; kb, kilobases; mpf, months post fertilisation; n, number of embryos; PAC, parachordal chain; PAM, protospacer adjustment motif; SBMO, splice-blocking morpholino; sgRNA, short guide RNA; U, undigested control;.

    Article Snippet: Frozen embryos were thawed and homogenised in 100 μl of protein extraction buffer (1% IGEPAL, 150 mM NaCl, 20 mM Tris pH 7.5, 2 mM EDTA, 50 mM NaF, 1mM sodium pyrophosphate) with 1× cOmplete Mini Protease Inhibitor Cocktail (1183615300, Roche) overnight at 4°C with rotation.

    Techniques: CRISPR, Mutagenesis, Injection, Fluorescence, Two Tailed Test, Amplification, Polymerase Chain Reaction, Variant Assay, Fluorescence In Situ Hybridization, Blocking Assay