minelute qiagen pcr purification kit  (Qiagen)


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    Name:
    MinElute PCR Purification Kit
    Description:
    For purification of up to 5 μg PCR products 70 bp to 4 kb in low elution volumes Kit contents Qiagen MinElute PCR Purification Kit 50 MinElute Spin Columns 5g Binding Capacity 10L Elution Volume Tube Format Silica Technology 70 bp to 4 kb Fragment Size Manual Processing DNA Sample Fast Procedure and Easy Handling High Reproducible Recoveries For Purification of up to 5μg PCR Products in Low Elution Volumes Includes 50 MinElute Spin Columns Buffers 2mL Collection Tubes Benefits Very small elution volumes Fast procedure and easy handling High reproducible recoveries Gel loading dye for convenient sample analysis
    Catalog Number:
    28004
    Price:
    134
    Category:
    MinElute PCR Purification Kit
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    Structured Review

    Qiagen minelute qiagen pcr purification kit
    MinElute PCR Purification Kit
    For purification of up to 5 μg PCR products 70 bp to 4 kb in low elution volumes Kit contents Qiagen MinElute PCR Purification Kit 50 MinElute Spin Columns 5g Binding Capacity 10L Elution Volume Tube Format Silica Technology 70 bp to 4 kb Fragment Size Manual Processing DNA Sample Fast Procedure and Easy Handling High Reproducible Recoveries For Purification of up to 5μg PCR Products in Low Elution Volumes Includes 50 MinElute Spin Columns Buffers 2mL Collection Tubes Benefits Very small elution volumes Fast procedure and easy handling High reproducible recoveries Gel loading dye for convenient sample analysis
    https://www.bioz.com/result/minelute qiagen pcr purification kit/product/Qiagen
    Average 95 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    minelute qiagen pcr purification kit - by Bioz Stars, 2020-02
    95/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Identification and partial characterization of a novel serpin from Eudiplozoon nipponicum (Monogenea, Polyopisthocotylea)
    Article Snippet: .. The PCR products (1200 bp) were resolved in 1% agarose gel, purified by a MinElute PCR Purification Kit (Qiagen) and sub-cloned into a pJET1.2 cloning vector (CloneJET PCR Cloning Kit, Thermo Scientific), which was used to transform E. coli XL-1 Blue (Novagen). pJET1.2 constructs were isolated using a High Pure Plasmid Isolation Kit (Roche) and sequenced with pJET1.2 forward and reverse primers from the kit (DNA Sequencing Laboratory, Faculty of Science, Charles University, Prague). .. Expression vector pET-22b(+) containing the EnSerp1 insert (1200 bp) was synthesised by GeneScript company, with the coding sequence for His-tag on C-terminus.

    Article Title: Compound heterozygous variants in the multiple PDZ domain protein (MPDZ) cause a case of mild non-progressive communicating hydrocephalus
    Article Snippet: To demonstrate the splice-effect of the identified variant (c.394G > A) on the mRNA level, exon 5 and parts of its flanking introns were sub-cloned into the mini-cassette vector using BSTEII ( GGTCACC) and NheI ( GCTAGC) restriction enzymes (New England Biolabs), they were included in the following cloning primers: Forward primer: 5’-ATGTG GGTCACC ACACTTACTAAG-3′, Reverse primer: 5′- TATTATGGGTCATT GCTAGC ATGG-3′. .. After amplifying the desired genomic fragment of MPDZ gene which was around 647 bps, it was further purified using MinElute PCR Purification Kit (Qiagen.

    Article Title: Biosynthetically-Intriguing Chlorinated Lipophilic Metabolites from Geographically Distant Tropical Marine Cyanobacteria
    Article Snippet: .. PCR products were purified using a MinElute® PCR Purification Kit (Qiagen) before subcloning using the Zero Blunt® TOPO® PCR Cloning Kit (Invitrogen) following the manufacturer’s specifications. .. Plasmid DNA was isolated using the QIAprep® Spin Miniprep Kit (Qiagen) and sequenced with M13 primers.

    Centrifugation:

    Article Title: Disruption of mesoderm formation during cardiac differentiation due to developmental exposure to 13-cis-retinoic acid
    Article Snippet: Cell pellets were re-suspended in 25 μl of ice-cold lysis buffer (10 mM Tris-HCl, 10 mM NaCl, 3 mM MgCl2 , and 0.1% Igepal CA-630, pH 7.4), and nuclei were pelleted by centrifugation for 30 min at 500 g and 4 °C. .. The reaction was incubated at 37 °C for 30 min, and subsequently the reaction mixture was purified using MinElute PCR Purification Kit (Qiagen).

    Amplification:

    Article Title: Interleukin 2 modulates thymic-derived regulatory T cell epigenetic landscape
    Article Snippet: Transposed DNA fragments were purified using Qiagen Mini-Elute Kit and PCR amplified using NEB Next High Fidelity 2× PCR master mix (New England Labs) with dual indexes primers (Illumina Nextera).). .. SATB1-bound DNA was then eluted from the beads and reverse cross-linked by incubating the beads pellet in 200 µl of IP elution buffer (1% SDS and 0.1 M NaHCO3 ) at 65 °C overnight and further purified with MinElute PCR Purification Kit (QIAGEN).

    Article Title: Pseudomonas-Specific NGS Assay Provides Insight Into Abundance and Dynamics of Pseudomonas Species Including P. aeruginosa in a Cooling Tower
    Article Snippet: After the second PCR amplification, the libraries were size-selected by gel electrophoresis on a 2% agarose gel pre-stained with GelRed Nucleic Acid Gel Stain (Biotium, Hayward, CA, United States), and recovered using a slightly modified QIAquick Gel Extraction Kit (Qiagen, Hilden, Germany) protocol ( ). .. Subsequently, pooled libraries were purified by the MinElute PCR Purification Kit (Qiagen, Hilden, Germany).

    Article Title: Disruption of mesoderm formation during cardiac differentiation due to developmental exposure to 13-cis-retinoic acid
    Article Snippet: The reaction was incubated at 37 °C for 30 min, and subsequently the reaction mixture was purified using MinElute PCR Purification Kit (Qiagen). .. The purified transposed DNA was amplified with NEBNext High-Fidelity 2 X PCR Master Mix (New England Biolabs) and custom-designed primers with barcodes .

    Article Title: First record of the nematode Libyostrongylus dentatus Hoberg, Lloyd Omar, 1995 (Trichostrongylidae) in ostriches (Struthio camelus Linnaeus, 1758) (Struthionidae) outside the Americas
    Article Snippet: Paragraph title: DNA extraction, PCR amplification and sequencing ... For PCR product purification, a MinElute PCR purification kit (Qiagen) was used according to the manufacturer’s protocol.

    Article Title: Biosynthetically-Intriguing Chlorinated Lipophilic Metabolites from Geographically Distant Tropical Marine Cyanobacteria
    Article Snippet: The PCR reactions were performed using the following gradient: initial denaturation for 2 min at 95°C, 25 cycles of amplification, followed by 20 sec at 95°C, 20 sec at 55°C and 1.5 min at 72 °C, and final elongation for 3 min at 72°C. .. PCR products were purified using a MinElute® PCR Purification Kit (Qiagen) before subcloning using the Zero Blunt® TOPO® PCR Cloning Kit (Invitrogen) following the manufacturer’s specifications.

    Article Title: Nitrate decreases ruminal methane production with slight changes to ruminal methanogen composition of nitrate-adapted steers
    Article Snippet: .. Amplicon purification was performed using a Qiagen MinElute PCR purification kit (Qiagen, Valencia, CA, USA) and the quantity of amplicon libraries was estimated with the Qubitds DNA HS assay using a Qubit 2.0 instrument (Life Technologies, USA). .. Subsequently, Illumina paired-end sequencing libraries were constructed from the purified PCR products using a NEB Next® Ultra™ DNA Library Prep Kit for Illumina (NEB, USA).

    Article Title: A phylogenetic framework facilitates Y-STR variant discovery and classification via massively parallel sequencing
    Article Snippet: .. Amplified products were purified using the MinElute® PCR purification kit (Qiagen), then quantified using the Qubit® dsDNA BR kit on the Qubit® 2.0 fluorometer. .. Library preparation was performed on ∼500 ng product per sample using the TruSeq® DNA PCR-free LT (24-plex) and HT (96-plex) sample preparation reagents (Illumina).

    Article Title: Multiple zoonotic helminth infections in domestic dogs in a rural area of Khuzestan Province in Iran
    Article Snippet: For species identification of amplified 267 and 117 bp fragments, PCR-positive samples were subjected to single PCR using Cest3 and Cest5 primer pairs for Taenia spp. and Cest4 and Cest5 primer pairs for E. granulosus . .. PCR products were purified using the MinElute PCR purification Kit (Qiagen, Germany) according to the manufacturer’s instructions and directly sequenced at the Bioneer Co. (Daejeon, South Korea) with the primers Cest4 and Cest5seq for E. granulosus s.s. and Taenia spp., respectively.

    Synthesized:

    Article Title: Pseudomonas-Specific NGS Assay Provides Insight Into Abundance and Dynamics of Pseudomonas Species Including P. aeruginosa in a Cooling Tower
    Article Snippet: All primers were synthesized and HPSF purified by Eurofins MWG Operon (Ebersberg, Germany). .. Subsequently, pooled libraries were purified by the MinElute PCR Purification Kit (Qiagen, Hilden, Germany).

    Picogreen Assay:

    Article Title: Pseudomonas-Specific NGS Assay Provides Insight Into Abundance and Dynamics of Pseudomonas Species Including P. aeruginosa in a Cooling Tower
    Article Snippet: The DNA concentration of the extracted amplicons was quantified by Quant-iT Picogreen dsDNA assay kit (Life Technologies, Oregon, United States) on a VICTOR X 3 2030 Multilabel Plate Reader (Perkin Elmer, Germany) to allow, when required, equimolar pooling. .. Subsequently, pooled libraries were purified by the MinElute PCR Purification Kit (Qiagen, Hilden, Germany).

    Quantitative RT-PCR:

    Article Title: An HIV-Encoded Antisense Long Noncoding RNA Epigenetically Regulates Viral Transcription
    Article Snippet: To determine induction of a nonspecific immune response, RNA was collected as described above 24 hours following treatment with 10 μg/ml poly(I:C; Sigma, MO, USA) and IFN-β induction was determined by qRT-PCR using the IFN-β specific primers: IFN-β F: 5′-tccaaattgctctcctgttgtgct-3′ and IFN-β R: 5′-ccacaggagcttctgacactgaaaa-3′. p24 analysis. .. ChIP eluates were purified using the MinElute PCR purification kit (Qiagen) and analyzed by qPCR.

    Real-time Polymerase Chain Reaction:

    Article Title: An HIV-Encoded Antisense Long Noncoding RNA Epigenetically Regulates Viral Transcription
    Article Snippet: .. ChIP eluates were purified using the MinElute PCR purification kit (Qiagen) and analyzed by qPCR. ..

    Article Title: A phylogenetic framework facilitates Y-STR variant discovery and classification via massively parallel sequencing
    Article Snippet: Amplified products were purified using the MinElute® PCR purification kit (Qiagen), then quantified using the Qubit® dsDNA BR kit on the Qubit® 2.0 fluorometer. .. Prepared libraries were quantified using the KAPA Library Quantification Kit for Illumina® platforms (KAPA Biosystems) with the LightCycler®480 (Roche) real-time PCR system following the manufacturers' recommendations.

    Article Title: Assessment of the gorilla gut virome in association with natural simian immunodeficiency virus infection
    Article Snippet: The MinElute PCR Purification Kit (QIAGEN) and the Agencourt AMPure XP System: PCR Purification kit (Beckman Coulter, CA, USA) were used to purify PCR-amplified products whenever requested by the ScriptSeq™ kit protocol. .. Quality control and absolute quantification of libraries were carried out in the ECO™ system (Eco Real-Time PCR System, Illumina® ) using the KAPA Library Quantification kit (KAPA Biosystems, MA, USA), according to the manufacturers’ specifications.

    Incubation:

    Article Title: Interleukin 2 modulates thymic-derived regulatory T cell epigenetic landscape
    Article Snippet: Sorted cells were next cross-linked in 1% (wt/vol) formaldehyde solution for 30 min. Cross-linked DNA was lysed in Farnham lysis buffer (5 mM PIPES pH 8.0, 85 mM KCl and 0.5% NP-40), fragmented in RIPA buffer (1% NP-40, 0.5% sodium deoxycholate, and 0.1% SDS in 1×PBS) using Bioruptor (Diagenode), and incubated overnight at 4 °C with 100 µL of DynaBeads M-280 Sheep anti-Rabbit IgG magnet beads (Invitrogen) preincubated with 20 µg of anti-SATB1 antibody (Abcam, ab70004). .. SATB1-bound DNA was then eluted from the beads and reverse cross-linked by incubating the beads pellet in 200 µl of IP elution buffer (1% SDS and 0.1 M NaHCO3 ) at 65 °C overnight and further purified with MinElute PCR Purification Kit (QIAGEN).

    Article Title: Disruption of mesoderm formation during cardiac differentiation due to developmental exposure to 13-cis-retinoic acid
    Article Snippet: .. The reaction was incubated at 37 °C for 30 min, and subsequently the reaction mixture was purified using MinElute PCR Purification Kit (Qiagen). .. The purified transposed DNA was amplified with NEBNext High-Fidelity 2 X PCR Master Mix (New England Biolabs) and custom-designed primers with barcodes .

    Article Title: Biosynthetically-Intriguing Chlorinated Lipophilic Metabolites from Geographically Distant Tropical Marine Cyanobacteria
    Article Snippet: The biomass was pretreated using TE (10 mM Tris; 0.1M EDTA; 0.5 % SDS; 20 µg/mL RNase)/lysozyme (1 mg/mL) at 37°C for 30 min followed by incubation with proteinase K (0.5 mg/mL) at 50°C for 1 h. Genomic DNA was extracted using the Wizard® Genomic DNA Purification Kit (Promega) following the manufacturer’s specifications. .. PCR products were purified using a MinElute® PCR Purification Kit (Qiagen) before subcloning using the Zero Blunt® TOPO® PCR Cloning Kit (Invitrogen) following the manufacturer’s specifications.

    Article Title: DNMT3A and TET1 cooperate to regulate promoter epigenetic landscapes in mouse embryonic stem cells
    Article Snippet: After incubation of chromatin and Dynabeads Protein A-antibody complex overnight at 4 °C, washes were done in the following order: 1 × low salt buffer (20 mM Tris-HCl pH 8.0, 0.1% SDS, 2 mM EDTA, 150 mM NaCl, 1% Triton X-100), 2 × high salt buffer (same as above), 2 × LiCl buffer (same as above), and 1 × TE buffer. .. Then cross-linking was reversed at 68 °C and immunoprecipitated DNA was purified by MinElute PCR purification Kit (Qiagen).

    Article Title: CRISPR-Cap: multiplexed double-stranded DNA enrichment based on the CRISPR system
    Article Snippet: .. We incubated the solution containing the released DNA for 5 min at room temperature and then purified the DNA using the MinElute PCR Purification Kit (QIAGEN) to remove any remaining SDS. ..

    Gel Extraction:

    Article Title: Pseudomonas-Specific NGS Assay Provides Insight Into Abundance and Dynamics of Pseudomonas Species Including P. aeruginosa in a Cooling Tower
    Article Snippet: After the second PCR amplification, the libraries were size-selected by gel electrophoresis on a 2% agarose gel pre-stained with GelRed Nucleic Acid Gel Stain (Biotium, Hayward, CA, United States), and recovered using a slightly modified QIAquick Gel Extraction Kit (Qiagen, Hilden, Germany) protocol ( ). .. Subsequently, pooled libraries were purified by the MinElute PCR Purification Kit (Qiagen, Hilden, Germany).

    Article Title: Compound heterozygous variants in the multiple PDZ domain protein (MPDZ) cause a case of mild non-progressive communicating hydrocephalus
    Article Snippet: After amplifying the desired genomic fragment of MPDZ gene which was around 647 bps, it was further purified using MinElute PCR Purification Kit (Qiagen. .. The digested vector was then gel-purified using MinElute Gel Extraction Kit (Qiagen.

    Expressing:

    Article Title: Identification and partial characterization of a novel serpin from Eudiplozoon nipponicum (Monogenea, Polyopisthocotylea)
    Article Snippet: The PCR products (1200 bp) were resolved in 1% agarose gel, purified by a MinElute PCR Purification Kit (Qiagen) and sub-cloned into a pJET1.2 cloning vector (CloneJET PCR Cloning Kit, Thermo Scientific), which was used to transform E. coli XL-1 Blue (Novagen). pJET1.2 constructs were isolated using a High Pure Plasmid Isolation Kit (Roche) and sequenced with pJET1.2 forward and reverse primers from the kit (DNA Sequencing Laboratory, Faculty of Science, Charles University, Prague). .. Expression vector pET-22b(+) containing the EnSerp1 insert (1200 bp) was synthesised by GeneScript company, with the coding sequence for His-tag on C-terminus.

    Modification:

    Article Title: Pseudomonas-Specific NGS Assay Provides Insight Into Abundance and Dynamics of Pseudomonas Species Including P. aeruginosa in a Cooling Tower
    Article Snippet: After the second PCR amplification, the libraries were size-selected by gel electrophoresis on a 2% agarose gel pre-stained with GelRed Nucleic Acid Gel Stain (Biotium, Hayward, CA, United States), and recovered using a slightly modified QIAquick Gel Extraction Kit (Qiagen, Hilden, Germany) protocol ( ). .. Subsequently, pooled libraries were purified by the MinElute PCR Purification Kit (Qiagen, Hilden, Germany).

    Article Title: Biosynthetically-Intriguing Chlorinated Lipophilic Metabolites from Geographically Distant Tropical Marine Cyanobacteria
    Article Snippet: The 16S rRNA genes were PCR-amplified from isolated DNA using the modified lineage-specific primers, CYA106F 5’-CGGACGGGTGAGTAACGCGTGA -3’ and CSL1445R 5’-GGTAACGACTTCGGGCGTG -3’. .. PCR products were purified using a MinElute® PCR Purification Kit (Qiagen) before subcloning using the Zero Blunt® TOPO® PCR Cloning Kit (Invitrogen) following the manufacturer’s specifications.

    Transformation Assay:

    Article Title: Identification and partial characterization of a novel serpin from Eudiplozoon nipponicum (Monogenea, Polyopisthocotylea)
    Article Snippet: The PCR products (1200 bp) were resolved in 1% agarose gel, purified by a MinElute PCR Purification Kit (Qiagen) and sub-cloned into a pJET1.2 cloning vector (CloneJET PCR Cloning Kit, Thermo Scientific), which was used to transform E. coli XL-1 Blue (Novagen). pJET1.2 constructs were isolated using a High Pure Plasmid Isolation Kit (Roche) and sequenced with pJET1.2 forward and reverse primers from the kit (DNA Sequencing Laboratory, Faculty of Science, Charles University, Prague). .. E. coli TOP10 cells were transformed by this construct for DNA plasmid propagation (heat shock 42 °C for 45 s).

    Flow Cytometry:

    Article Title: Interleukin 2 modulates thymic-derived regulatory T cell epigenetic landscape
    Article Snippet: After staining the remaining cells with fluorescent labeled antibodies against CD4 (GK1.5) and CD8α (53-6.7), we flow-sorted 2 × 106 CD4+ CD8αneg SP thymocytes. .. SATB1-bound DNA was then eluted from the beads and reverse cross-linked by incubating the beads pellet in 200 µl of IP elution buffer (1% SDS and 0.1 M NaHCO3 ) at 65 °C overnight and further purified with MinElute PCR Purification Kit (QIAGEN).

    Concentration Assay:

    Article Title: Pseudomonas-Specific NGS Assay Provides Insight Into Abundance and Dynamics of Pseudomonas Species Including P. aeruginosa in a Cooling Tower
    Article Snippet: The DNA concentration of the extracted amplicons was quantified by Quant-iT Picogreen dsDNA assay kit (Life Technologies, Oregon, United States) on a VICTOR X 3 2030 Multilabel Plate Reader (Perkin Elmer, Germany) to allow, when required, equimolar pooling. .. Subsequently, pooled libraries were purified by the MinElute PCR Purification Kit (Qiagen, Hilden, Germany).

    Article Title: An HIV-Encoded Antisense Long Noncoding RNA Epigenetically Regulates Viral Transcription
    Article Snippet: Samples were neutralized by lysis in Triton X-100 at a final concentration of 0.5%. .. ChIP eluates were purified using the MinElute PCR purification kit (Qiagen) and analyzed by qPCR.

    Article Title: Disruption of mesoderm formation during cardiac differentiation due to developmental exposure to 13-cis-retinoic acid
    Article Snippet: The reaction was incubated at 37 °C for 30 min, and subsequently the reaction mixture was purified using MinElute PCR Purification Kit (Qiagen). .. DNA concentration was measured with a Qubit Fluorometer (Thermo Fisher Scientific) and library sizes were determined using an Agilent High Sensitivity DNA kit on an Agilent 2100 Bioanalyzer.

    DNA HS Assay:

    Article Title: Nitrate decreases ruminal methane production with slight changes to ruminal methanogen composition of nitrate-adapted steers
    Article Snippet: .. Amplicon purification was performed using a Qiagen MinElute PCR purification kit (Qiagen, Valencia, CA, USA) and the quantity of amplicon libraries was estimated with the Qubitds DNA HS assay using a Qubit 2.0 instrument (Life Technologies, USA). .. Subsequently, Illumina paired-end sequencing libraries were constructed from the purified PCR products using a NEB Next® Ultra™ DNA Library Prep Kit for Illumina (NEB, USA).

    DNA Sequencing:

    Article Title: Identification and partial characterization of a novel serpin from Eudiplozoon nipponicum (Monogenea, Polyopisthocotylea)
    Article Snippet: .. The PCR products (1200 bp) were resolved in 1% agarose gel, purified by a MinElute PCR Purification Kit (Qiagen) and sub-cloned into a pJET1.2 cloning vector (CloneJET PCR Cloning Kit, Thermo Scientific), which was used to transform E. coli XL-1 Blue (Novagen). pJET1.2 constructs were isolated using a High Pure Plasmid Isolation Kit (Roche) and sequenced with pJET1.2 forward and reverse primers from the kit (DNA Sequencing Laboratory, Faculty of Science, Charles University, Prague). .. Expression vector pET-22b(+) containing the EnSerp1 insert (1200 bp) was synthesised by GeneScript company, with the coding sequence for His-tag on C-terminus.

    Sequencing:

    Article Title: Pseudomonas-Specific NGS Assay Provides Insight Into Abundance and Dynamics of Pseudomonas Species Including P. aeruginosa in a Cooling Tower
    Article Snippet: Subsequently, pooled libraries were purified by the MinElute PCR Purification Kit (Qiagen, Hilden, Germany). .. The sequencing of the libraries was completed by the Genome Analysis Department of the HZI, using the Illumina MiSeq platform (V2 chemistry, 250 bp paired-end run).

    Article Title: Identification and partial characterization of a novel serpin from Eudiplozoon nipponicum (Monogenea, Polyopisthocotylea)
    Article Snippet: The PCR products (1200 bp) were resolved in 1% agarose gel, purified by a MinElute PCR Purification Kit (Qiagen) and sub-cloned into a pJET1.2 cloning vector (CloneJET PCR Cloning Kit, Thermo Scientific), which was used to transform E. coli XL-1 Blue (Novagen). pJET1.2 constructs were isolated using a High Pure Plasmid Isolation Kit (Roche) and sequenced with pJET1.2 forward and reverse primers from the kit (DNA Sequencing Laboratory, Faculty of Science, Charles University, Prague). .. Expression vector pET-22b(+) containing the EnSerp1 insert (1200 bp) was synthesised by GeneScript company, with the coding sequence for His-tag on C-terminus.

    Article Title: First record of the nematode Libyostrongylus dentatus Hoberg, Lloyd Omar, 1995 (Trichostrongylidae) in ostriches (Struthio camelus Linnaeus, 1758) (Struthionidae) outside the Americas
    Article Snippet: Paragraph title: DNA extraction, PCR amplification and sequencing ... For PCR product purification, a MinElute PCR purification kit (Qiagen) was used according to the manufacturer’s protocol.

    Article Title: Nitrate decreases ruminal methane production with slight changes to ruminal methanogen composition of nitrate-adapted steers
    Article Snippet: Paragraph title: Hiseq sequencing and data analyses ... Amplicon purification was performed using a Qiagen MinElute PCR purification kit (Qiagen, Valencia, CA, USA) and the quantity of amplicon libraries was estimated with the Qubitds DNA HS assay using a Qubit 2.0 instrument (Life Technologies, USA).

    Article Title: A phylogenetic framework facilitates Y-STR variant discovery and classification via massively parallel sequencing
    Article Snippet: Paragraph title: 2.3. Library preparation and sequencing ... Amplified products were purified using the MinElute® PCR purification kit (Qiagen), then quantified using the Qubit® dsDNA BR kit on the Qubit® 2.0 fluorometer.

    Article Title: Multiple zoonotic helminth infections in domestic dogs in a rural area of Khuzestan Province in Iran
    Article Snippet: Paragraph title: Sequencing ... PCR products were purified using the MinElute PCR purification Kit (Qiagen, Germany) according to the manufacturer’s instructions and directly sequenced at the Bioneer Co. (Daejeon, South Korea) with the primers Cest4 and Cest5seq for E. granulosus s.s. and Taenia spp., respectively.

    Binding Assay:

    Article Title: CRISPR-Cap: multiplexed double-stranded DNA enrichment based on the CRISPR system
    Article Snippet: We mixed the washed beads directly with the product from the cleavage step and incubated the mixture for 10 min at room temperature to allow binding of the Cas9–DNA complexes to the streptavidin. .. We incubated the solution containing the released DNA for 5 min at room temperature and then purified the DNA using the MinElute PCR Purification Kit (QIAGEN) to remove any remaining SDS.

    Methylated DNA Immunoprecipitation:

    Article Title: Comprehensive whole DNA methylome analysis by integrating MeDIP-seq and MRE-seq
    Article Snippet: Paragraph title: 3.1.4. MeDIP ... Purify DNA with Qiagen MinElute PCR Purification Kit.

    Multiplexing:

    Article Title: Pseudomonas-Specific NGS Assay Provides Insight Into Abundance and Dynamics of Pseudomonas Species Including P. aeruginosa in a Cooling Tower
    Article Snippet: The reverse primer integrated an unique index selected from twelve used in this study, which are detailed in Illumina library preparation protocols, in order to allow multiplexing of samples. .. Subsequently, pooled libraries were purified by the MinElute PCR Purification Kit (Qiagen, Hilden, Germany).

    Nucleic Acid Electrophoresis:

    Article Title: Pseudomonas-Specific NGS Assay Provides Insight Into Abundance and Dynamics of Pseudomonas Species Including P. aeruginosa in a Cooling Tower
    Article Snippet: After the second PCR amplification, the libraries were size-selected by gel electrophoresis on a 2% agarose gel pre-stained with GelRed Nucleic Acid Gel Stain (Biotium, Hayward, CA, United States), and recovered using a slightly modified QIAquick Gel Extraction Kit (Qiagen, Hilden, Germany) protocol ( ). .. Subsequently, pooled libraries were purified by the MinElute PCR Purification Kit (Qiagen, Hilden, Germany).

    Article Title: Disruption of mesoderm formation during cardiac differentiation due to developmental exposure to 13-cis-retinoic acid
    Article Snippet: The reaction was incubated at 37 °C for 30 min, and subsequently the reaction mixture was purified using MinElute PCR Purification Kit (Qiagen). .. Gel electrophoresis was used to remove primer dimers from the PCR products with 2% E-Gel EX Agarose Gels (Thermo Fisher Scientific), and then the PCR products were purified using QIAquick PCR Purification Kit (Qiagen).

    Magnetic Beads:

    Article Title: DNMT3A and TET1 cooperate to regulate promoter epigenetic landscapes in mouse embryonic stem cells
    Article Snippet: Immunoprecipitation was performed overnight at 4 °C using M280-streptavidin (Thermo Fisher) magnetic beads. .. Then cross-linking was reversed at 68 °C and immunoprecipitated DNA was purified by MinElute PCR purification Kit (Qiagen).

    Article Title: CRISPR-Cap: multiplexed double-stranded DNA enrichment based on the CRISPR system
    Article Snippet: Paragraph title: Sorting step of CRISPR-Cap using streptavidin magnetic beads ... We incubated the solution containing the released DNA for 5 min at room temperature and then purified the DNA using the MinElute PCR Purification Kit (QIAGEN) to remove any remaining SDS.

    Mutagenesis:

    Article Title: Compound heterozygous variants in the multiple PDZ domain protein (MPDZ) cause a case of mild non-progressive communicating hydrocephalus
    Article Snippet: Paragraph title: Generation of the minigene construct, mutagenesis and transfection ... After amplifying the desired genomic fragment of MPDZ gene which was around 647 bps, it was further purified using MinElute PCR Purification Kit (Qiagen.

    Isolation:

    Article Title: An HIV-Encoded Antisense Long Noncoding RNA Epigenetically Regulates Viral Transcription
    Article Snippet: ChIP eluates were purified using the MinElute PCR purification kit (Qiagen) and analyzed by qPCR. .. Directional RT primer sequences are listed in the RNA isolation and analysis methods section above. qPCR to detect enrichment at the 5′LTR utilized primer set 3: primer set 3 F: 5′-ctttccgctggggactttccagg-3′; primer set 3 R: 5′-ccagagagacccagtacaggcaaaaagcag-3′.

    Article Title: Identification and partial characterization of a novel serpin from Eudiplozoon nipponicum (Monogenea, Polyopisthocotylea)
    Article Snippet: .. The PCR products (1200 bp) were resolved in 1% agarose gel, purified by a MinElute PCR Purification Kit (Qiagen) and sub-cloned into a pJET1.2 cloning vector (CloneJET PCR Cloning Kit, Thermo Scientific), which was used to transform E. coli XL-1 Blue (Novagen). pJET1.2 constructs were isolated using a High Pure Plasmid Isolation Kit (Roche) and sequenced with pJET1.2 forward and reverse primers from the kit (DNA Sequencing Laboratory, Faculty of Science, Charles University, Prague). .. Expression vector pET-22b(+) containing the EnSerp1 insert (1200 bp) was synthesised by GeneScript company, with the coding sequence for His-tag on C-terminus.

    Article Title: Biosynthetically-Intriguing Chlorinated Lipophilic Metabolites from Geographically Distant Tropical Marine Cyanobacteria
    Article Snippet: The 16S rRNA genes were PCR-amplified from isolated DNA using the modified lineage-specific primers, CYA106F 5’-CGGACGGGTGAGTAACGCGTGA -3’ and CSL1445R 5’-GGTAACGACTTCGGGCGTG -3’. .. PCR products were purified using a MinElute® PCR Purification Kit (Qiagen) before subcloning using the Zero Blunt® TOPO® PCR Cloning Kit (Invitrogen) following the manufacturer’s specifications.

    Article Title: CRISPR-Cap: multiplexed double-stranded DNA enrichment based on the CRISPR system
    Article Snippet: We then isolated the beads using a magnetic stand and discarded the supernatant. .. We incubated the solution containing the released DNA for 5 min at room temperature and then purified the DNA using the MinElute PCR Purification Kit (QIAGEN) to remove any remaining SDS.

    Subcloning:

    Article Title: Biosynthetically-Intriguing Chlorinated Lipophilic Metabolites from Geographically Distant Tropical Marine Cyanobacteria
    Article Snippet: .. PCR products were purified using a MinElute® PCR Purification Kit (Qiagen) before subcloning using the Zero Blunt® TOPO® PCR Cloning Kit (Invitrogen) following the manufacturer’s specifications. .. Plasmid DNA was isolated using the QIAprep® Spin Miniprep Kit (Qiagen) and sequenced with M13 primers.

    Transfection:

    Article Title: Compound heterozygous variants in the multiple PDZ domain protein (MPDZ) cause a case of mild non-progressive communicating hydrocephalus
    Article Snippet: Paragraph title: Generation of the minigene construct, mutagenesis and transfection ... After amplifying the desired genomic fragment of MPDZ gene which was around 647 bps, it was further purified using MinElute PCR Purification Kit (Qiagen.

    Labeling:

    Article Title: Interleukin 2 modulates thymic-derived regulatory T cell epigenetic landscape
    Article Snippet: After staining the remaining cells with fluorescent labeled antibodies against CD4 (GK1.5) and CD8α (53-6.7), we flow-sorted 2 × 106 CD4+ CD8αneg SP thymocytes. .. SATB1-bound DNA was then eluted from the beads and reverse cross-linked by incubating the beads pellet in 200 µl of IP elution buffer (1% SDS and 0.1 M NaHCO3 ) at 65 °C overnight and further purified with MinElute PCR Purification Kit (QIAGEN).

    Purification:

    Article Title: Interleukin 2 modulates thymic-derived regulatory T cell epigenetic landscape
    Article Snippet: .. SATB1-bound DNA was then eluted from the beads and reverse cross-linked by incubating the beads pellet in 200 µl of IP elution buffer (1% SDS and 0.1 M NaHCO3 ) at 65 °C overnight and further purified with MinElute PCR Purification Kit (QIAGEN). ..

    Article Title: Pseudomonas-Specific NGS Assay Provides Insight Into Abundance and Dynamics of Pseudomonas Species Including P. aeruginosa in a Cooling Tower
    Article Snippet: .. Subsequently, pooled libraries were purified by the MinElute PCR Purification Kit (Qiagen, Hilden, Germany). .. Molarity was quantified and library fragment size confirmed with Agilent Bioanalyzer.

    Article Title: An HIV-Encoded Antisense Long Noncoding RNA Epigenetically Regulates Viral Transcription
    Article Snippet: .. ChIP eluates were purified using the MinElute PCR purification kit (Qiagen) and analyzed by qPCR. ..

    Article Title: Disruption of mesoderm formation during cardiac differentiation due to developmental exposure to 13-cis-retinoic acid
    Article Snippet: .. The reaction was incubated at 37 °C for 30 min, and subsequently the reaction mixture was purified using MinElute PCR Purification Kit (Qiagen). .. The purified transposed DNA was amplified with NEBNext High-Fidelity 2 X PCR Master Mix (New England Biolabs) and custom-designed primers with barcodes .

    Article Title: Identification and partial characterization of a novel serpin from Eudiplozoon nipponicum (Monogenea, Polyopisthocotylea)
    Article Snippet: .. The PCR products (1200 bp) were resolved in 1% agarose gel, purified by a MinElute PCR Purification Kit (Qiagen) and sub-cloned into a pJET1.2 cloning vector (CloneJET PCR Cloning Kit, Thermo Scientific), which was used to transform E. coli XL-1 Blue (Novagen). pJET1.2 constructs were isolated using a High Pure Plasmid Isolation Kit (Roche) and sequenced with pJET1.2 forward and reverse primers from the kit (DNA Sequencing Laboratory, Faculty of Science, Charles University, Prague). .. Expression vector pET-22b(+) containing the EnSerp1 insert (1200 bp) was synthesised by GeneScript company, with the coding sequence for His-tag on C-terminus.

    Article Title: Comprehensive whole DNA methylome analysis by integrating MeDIP-seq and MRE-seq
    Article Snippet: .. Purify DNA with Qiagen MinElute PCR Purification Kit. ..

    Article Title: First record of the nematode Libyostrongylus dentatus Hoberg, Lloyd Omar, 1995 (Trichostrongylidae) in ostriches (Struthio camelus Linnaeus, 1758) (Struthionidae) outside the Americas
    Article Snippet: .. For PCR product purification, a MinElute PCR purification kit (Qiagen) was used according to the manufacturer’s protocol. .. Amplicons were directly sequenced in both directions using Big Dye Terminator v3.1 Cycle Sequencing Ready reaction kit (Applied Biosystems, Foster City, USA) as recommended by the supplier in a 3100 Automated DNA Sequencer (Applied Biosystems) located on the RPT01A/IOC- Fiocruz sequencing platform.

    Article Title: Compound heterozygous variants in the multiple PDZ domain protein (MPDZ) cause a case of mild non-progressive communicating hydrocephalus
    Article Snippet: .. After amplifying the desired genomic fragment of MPDZ gene which was around 647 bps, it was further purified using MinElute PCR Purification Kit (Qiagen. ..

    Article Title: Biosynthetically-Intriguing Chlorinated Lipophilic Metabolites from Geographically Distant Tropical Marine Cyanobacteria
    Article Snippet: .. PCR products were purified using a MinElute® PCR Purification Kit (Qiagen) before subcloning using the Zero Blunt® TOPO® PCR Cloning Kit (Invitrogen) following the manufacturer’s specifications. .. Plasmid DNA was isolated using the QIAprep® Spin Miniprep Kit (Qiagen) and sequenced with M13 primers.

    Article Title: Nitrate decreases ruminal methane production with slight changes to ruminal methanogen composition of nitrate-adapted steers
    Article Snippet: .. Amplicon purification was performed using a Qiagen MinElute PCR purification kit (Qiagen, Valencia, CA, USA) and the quantity of amplicon libraries was estimated with the Qubitds DNA HS assay using a Qubit 2.0 instrument (Life Technologies, USA). .. Subsequently, Illumina paired-end sequencing libraries were constructed from the purified PCR products using a NEB Next® Ultra™ DNA Library Prep Kit for Illumina (NEB, USA).

    Article Title: A phylogenetic framework facilitates Y-STR variant discovery and classification via massively parallel sequencing
    Article Snippet: .. Amplified products were purified using the MinElute® PCR purification kit (Qiagen), then quantified using the Qubit® dsDNA BR kit on the Qubit® 2.0 fluorometer. .. Library preparation was performed on ∼500 ng product per sample using the TruSeq® DNA PCR-free LT (24-plex) and HT (96-plex) sample preparation reagents (Illumina).

    Article Title: Multiple zoonotic helminth infections in domestic dogs in a rural area of Khuzestan Province in Iran
    Article Snippet: .. PCR products were purified using the MinElute PCR purification Kit (Qiagen, Germany) according to the manufacturer’s instructions and directly sequenced at the Bioneer Co. (Daejeon, South Korea) with the primers Cest4 and Cest5seq for E. granulosus s.s. and Taenia spp., respectively. .. The frequency of parasite occurrence in the samples and respective 95% confidence intervals (CI) were calculated using SPSS 18 software (SPSS Inc., Chicago, IL, USA).

    Article Title: DNMT3A and TET1 cooperate to regulate promoter epigenetic landscapes in mouse embryonic stem cells
    Article Snippet: .. Then cross-linking was reversed at 68 °C and immunoprecipitated DNA was purified by MinElute PCR purification Kit (Qiagen). .. H3K4me3 (Millipore, 07–473) and H3K27me3 (Millipore, 07–449; Cell signaling, 9733S) ChIP were performed as previously described [ ], with 3 million cells.

    Article Title: Assessment of the gorilla gut virome in association with natural simian immunodeficiency virus infection
    Article Snippet: .. The MinElute PCR Purification Kit (QIAGEN) and the Agencourt AMPure XP System: PCR Purification kit (Beckman Coulter, CA, USA) were used to purify PCR-amplified products whenever requested by the ScriptSeq™ kit protocol. .. DNA libraries were constructed using the Nextera® DNA Sample Preparation kit (Illumina® , CA, USA), following the manufacturer’s instructions.

    Article Title: CRISPR-Cap: multiplexed double-stranded DNA enrichment based on the CRISPR system
    Article Snippet: .. We incubated the solution containing the released DNA for 5 min at room temperature and then purified the DNA using the MinElute PCR Purification Kit (QIAGEN) to remove any remaining SDS. ..

    Polymerase Chain Reaction:

    Article Title: Interleukin 2 modulates thymic-derived regulatory T cell epigenetic landscape
    Article Snippet: .. SATB1-bound DNA was then eluted from the beads and reverse cross-linked by incubating the beads pellet in 200 µl of IP elution buffer (1% SDS and 0.1 M NaHCO3 ) at 65 °C overnight and further purified with MinElute PCR Purification Kit (QIAGEN). ..

    Article Title: Pseudomonas-Specific NGS Assay Provides Insight Into Abundance and Dynamics of Pseudomonas Species Including P. aeruginosa in a Cooling Tower
    Article Snippet: .. Subsequently, pooled libraries were purified by the MinElute PCR Purification Kit (Qiagen, Hilden, Germany). .. Molarity was quantified and library fragment size confirmed with Agilent Bioanalyzer.

    Article Title: An HIV-Encoded Antisense Long Noncoding RNA Epigenetically Regulates Viral Transcription
    Article Snippet: .. ChIP eluates were purified using the MinElute PCR purification kit (Qiagen) and analyzed by qPCR. ..

    Article Title: Disruption of mesoderm formation during cardiac differentiation due to developmental exposure to 13-cis-retinoic acid
    Article Snippet: .. The reaction was incubated at 37 °C for 30 min, and subsequently the reaction mixture was purified using MinElute PCR Purification Kit (Qiagen). .. The purified transposed DNA was amplified with NEBNext High-Fidelity 2 X PCR Master Mix (New England Biolabs) and custom-designed primers with barcodes .

    Article Title: Identification and partial characterization of a novel serpin from Eudiplozoon nipponicum (Monogenea, Polyopisthocotylea)
    Article Snippet: .. The PCR products (1200 bp) were resolved in 1% agarose gel, purified by a MinElute PCR Purification Kit (Qiagen) and sub-cloned into a pJET1.2 cloning vector (CloneJET PCR Cloning Kit, Thermo Scientific), which was used to transform E. coli XL-1 Blue (Novagen). pJET1.2 constructs were isolated using a High Pure Plasmid Isolation Kit (Roche) and sequenced with pJET1.2 forward and reverse primers from the kit (DNA Sequencing Laboratory, Faculty of Science, Charles University, Prague). .. Expression vector pET-22b(+) containing the EnSerp1 insert (1200 bp) was synthesised by GeneScript company, with the coding sequence for His-tag on C-terminus.

    Article Title: Comprehensive whole DNA methylome analysis by integrating MeDIP-seq and MRE-seq
    Article Snippet: .. Purify DNA with Qiagen MinElute PCR Purification Kit. ..

    Article Title: First record of the nematode Libyostrongylus dentatus Hoberg, Lloyd Omar, 1995 (Trichostrongylidae) in ostriches (Struthio camelus Linnaeus, 1758) (Struthionidae) outside the Americas
    Article Snippet: .. For PCR product purification, a MinElute PCR purification kit (Qiagen) was used according to the manufacturer’s protocol. .. Amplicons were directly sequenced in both directions using Big Dye Terminator v3.1 Cycle Sequencing Ready reaction kit (Applied Biosystems, Foster City, USA) as recommended by the supplier in a 3100 Automated DNA Sequencer (Applied Biosystems) located on the RPT01A/IOC- Fiocruz sequencing platform.

    Article Title: Compound heterozygous variants in the multiple PDZ domain protein (MPDZ) cause a case of mild non-progressive communicating hydrocephalus
    Article Snippet: .. After amplifying the desired genomic fragment of MPDZ gene which was around 647 bps, it was further purified using MinElute PCR Purification Kit (Qiagen. ..

    Article Title: Biosynthetically-Intriguing Chlorinated Lipophilic Metabolites from Geographically Distant Tropical Marine Cyanobacteria
    Article Snippet: .. PCR products were purified using a MinElute® PCR Purification Kit (Qiagen) before subcloning using the Zero Blunt® TOPO® PCR Cloning Kit (Invitrogen) following the manufacturer’s specifications. .. Plasmid DNA was isolated using the QIAprep® Spin Miniprep Kit (Qiagen) and sequenced with M13 primers.

    Article Title: Nitrate decreases ruminal methane production with slight changes to ruminal methanogen composition of nitrate-adapted steers
    Article Snippet: .. Amplicon purification was performed using a Qiagen MinElute PCR purification kit (Qiagen, Valencia, CA, USA) and the quantity of amplicon libraries was estimated with the Qubitds DNA HS assay using a Qubit 2.0 instrument (Life Technologies, USA). .. Subsequently, Illumina paired-end sequencing libraries were constructed from the purified PCR products using a NEB Next® Ultra™ DNA Library Prep Kit for Illumina (NEB, USA).

    Article Title: A phylogenetic framework facilitates Y-STR variant discovery and classification via massively parallel sequencing
    Article Snippet: .. Amplified products were purified using the MinElute® PCR purification kit (Qiagen), then quantified using the Qubit® dsDNA BR kit on the Qubit® 2.0 fluorometer. .. Library preparation was performed on ∼500 ng product per sample using the TruSeq® DNA PCR-free LT (24-plex) and HT (96-plex) sample preparation reagents (Illumina).

    Article Title: Multiple zoonotic helminth infections in domestic dogs in a rural area of Khuzestan Province in Iran
    Article Snippet: .. PCR products were purified using the MinElute PCR purification Kit (Qiagen, Germany) according to the manufacturer’s instructions and directly sequenced at the Bioneer Co. (Daejeon, South Korea) with the primers Cest4 and Cest5seq for E. granulosus s.s. and Taenia spp., respectively. .. The frequency of parasite occurrence in the samples and respective 95% confidence intervals (CI) were calculated using SPSS 18 software (SPSS Inc., Chicago, IL, USA).

    Article Title: DNMT3A and TET1 cooperate to regulate promoter epigenetic landscapes in mouse embryonic stem cells
    Article Snippet: .. Then cross-linking was reversed at 68 °C and immunoprecipitated DNA was purified by MinElute PCR purification Kit (Qiagen). .. H3K4me3 (Millipore, 07–473) and H3K27me3 (Millipore, 07–449; Cell signaling, 9733S) ChIP were performed as previously described [ ], with 3 million cells.

    Article Title: Assessment of the gorilla gut virome in association with natural simian immunodeficiency virus infection
    Article Snippet: .. The MinElute PCR Purification Kit (QIAGEN) and the Agencourt AMPure XP System: PCR Purification kit (Beckman Coulter, CA, USA) were used to purify PCR-amplified products whenever requested by the ScriptSeq™ kit protocol. .. DNA libraries were constructed using the Nextera® DNA Sample Preparation kit (Illumina® , CA, USA), following the manufacturer’s instructions.

    Article Title: CRISPR-Cap: multiplexed double-stranded DNA enrichment based on the CRISPR system
    Article Snippet: .. We incubated the solution containing the released DNA for 5 min at room temperature and then purified the DNA using the MinElute PCR Purification Kit (QIAGEN) to remove any remaining SDS. ..

    Selection:

    Article Title: Interleukin 2 modulates thymic-derived regulatory T cell epigenetic landscape
    Article Snippet: Single positive (SP) CD4+ thymocytes from thymus of WT and Il2ra mut/mut mice on two biological replicates were pre-enriched by negative selection using anti-CD8β (H35), anti-MHC-II (M5/114), anti-CD11b (M1/70) and anti-TER119. .. SATB1-bound DNA was then eluted from the beads and reverse cross-linked by incubating the beads pellet in 200 µl of IP elution buffer (1% SDS and 0.1 M NaHCO3 ) at 65 °C overnight and further purified with MinElute PCR Purification Kit (QIAGEN).

    Article Title: A phylogenetic framework facilitates Y-STR variant discovery and classification via massively parallel sequencing
    Article Snippet: Amplified products were purified using the MinElute® PCR purification kit (Qiagen), then quantified using the Qubit® dsDNA BR kit on the Qubit® 2.0 fluorometer. .. The manufacturer’s protocol was used, with an adjustment for the PowerSeq™ System (Promega), namely the use of the MinElute® PCR purification kit for size selection of amplicons.

    Construct:

    Article Title: Identification and partial characterization of a novel serpin from Eudiplozoon nipponicum (Monogenea, Polyopisthocotylea)
    Article Snippet: .. The PCR products (1200 bp) were resolved in 1% agarose gel, purified by a MinElute PCR Purification Kit (Qiagen) and sub-cloned into a pJET1.2 cloning vector (CloneJET PCR Cloning Kit, Thermo Scientific), which was used to transform E. coli XL-1 Blue (Novagen). pJET1.2 constructs were isolated using a High Pure Plasmid Isolation Kit (Roche) and sequenced with pJET1.2 forward and reverse primers from the kit (DNA Sequencing Laboratory, Faculty of Science, Charles University, Prague). .. Expression vector pET-22b(+) containing the EnSerp1 insert (1200 bp) was synthesised by GeneScript company, with the coding sequence for His-tag on C-terminus.

    Article Title: Compound heterozygous variants in the multiple PDZ domain protein (MPDZ) cause a case of mild non-progressive communicating hydrocephalus
    Article Snippet: Paragraph title: Generation of the minigene construct, mutagenesis and transfection ... After amplifying the desired genomic fragment of MPDZ gene which was around 647 bps, it was further purified using MinElute PCR Purification Kit (Qiagen.

    Article Title: Nitrate decreases ruminal methane production with slight changes to ruminal methanogen composition of nitrate-adapted steers
    Article Snippet: Amplicon purification was performed using a Qiagen MinElute PCR purification kit (Qiagen, Valencia, CA, USA) and the quantity of amplicon libraries was estimated with the Qubitds DNA HS assay using a Qubit 2.0 instrument (Life Technologies, USA). .. Subsequently, Illumina paired-end sequencing libraries were constructed from the purified PCR products using a NEB Next® Ultra™ DNA Library Prep Kit for Illumina (NEB, USA).

    Article Title: Assessment of the gorilla gut virome in association with natural simian immunodeficiency virus infection
    Article Snippet: The MinElute PCR Purification Kit (QIAGEN) and the Agencourt AMPure XP System: PCR Purification kit (Beckman Coulter, CA, USA) were used to purify PCR-amplified products whenever requested by the ScriptSeq™ kit protocol. .. DNA libraries were constructed using the Nextera® DNA Sample Preparation kit (Illumina® , CA, USA), following the manufacturer’s instructions.

    CRISPR:

    Article Title: CRISPR-Cap: multiplexed double-stranded DNA enrichment based on the CRISPR system
    Article Snippet: Paragraph title: Sorting step of CRISPR-Cap using streptavidin magnetic beads ... We incubated the solution containing the released DNA for 5 min at room temperature and then purified the DNA using the MinElute PCR Purification Kit (QIAGEN) to remove any remaining SDS.

    Agarose Gel Electrophoresis:

    Article Title: Pseudomonas-Specific NGS Assay Provides Insight Into Abundance and Dynamics of Pseudomonas Species Including P. aeruginosa in a Cooling Tower
    Article Snippet: After the second PCR amplification, the libraries were size-selected by gel electrophoresis on a 2% agarose gel pre-stained with GelRed Nucleic Acid Gel Stain (Biotium, Hayward, CA, United States), and recovered using a slightly modified QIAquick Gel Extraction Kit (Qiagen, Hilden, Germany) protocol ( ). .. Subsequently, pooled libraries were purified by the MinElute PCR Purification Kit (Qiagen, Hilden, Germany).

    Article Title: Identification and partial characterization of a novel serpin from Eudiplozoon nipponicum (Monogenea, Polyopisthocotylea)
    Article Snippet: .. The PCR products (1200 bp) were resolved in 1% agarose gel, purified by a MinElute PCR Purification Kit (Qiagen) and sub-cloned into a pJET1.2 cloning vector (CloneJET PCR Cloning Kit, Thermo Scientific), which was used to transform E. coli XL-1 Blue (Novagen). pJET1.2 constructs were isolated using a High Pure Plasmid Isolation Kit (Roche) and sequenced with pJET1.2 forward and reverse primers from the kit (DNA Sequencing Laboratory, Faculty of Science, Charles University, Prague). .. Expression vector pET-22b(+) containing the EnSerp1 insert (1200 bp) was synthesised by GeneScript company, with the coding sequence for His-tag on C-terminus.

    Mouse Assay:

    Article Title: Interleukin 2 modulates thymic-derived regulatory T cell epigenetic landscape
    Article Snippet: Single positive (SP) CD4+ thymocytes from thymus of WT and Il2ra mut/mut mice on two biological replicates were pre-enriched by negative selection using anti-CD8β (H35), anti-MHC-II (M5/114), anti-CD11b (M1/70) and anti-TER119. .. SATB1-bound DNA was then eluted from the beads and reverse cross-linked by incubating the beads pellet in 200 µl of IP elution buffer (1% SDS and 0.1 M NaHCO3 ) at 65 °C overnight and further purified with MinElute PCR Purification Kit (QIAGEN).

    Chromatin Immunoprecipitation:

    Article Title: An HIV-Encoded Antisense Long Noncoding RNA Epigenetically Regulates Viral Transcription
    Article Snippet: .. ChIP eluates were purified using the MinElute PCR purification kit (Qiagen) and analyzed by qPCR. ..

    Article Title: DNMT3A and TET1 cooperate to regulate promoter epigenetic landscapes in mouse embryonic stem cells
    Article Snippet: Paragraph title: Chromatin immunoprecipitation ... Then cross-linking was reversed at 68 °C and immunoprecipitated DNA was purified by MinElute PCR purification Kit (Qiagen).

    Plasmid Preparation:

    Article Title: Identification and partial characterization of a novel serpin from Eudiplozoon nipponicum (Monogenea, Polyopisthocotylea)
    Article Snippet: .. The PCR products (1200 bp) were resolved in 1% agarose gel, purified by a MinElute PCR Purification Kit (Qiagen) and sub-cloned into a pJET1.2 cloning vector (CloneJET PCR Cloning Kit, Thermo Scientific), which was used to transform E. coli XL-1 Blue (Novagen). pJET1.2 constructs were isolated using a High Pure Plasmid Isolation Kit (Roche) and sequenced with pJET1.2 forward and reverse primers from the kit (DNA Sequencing Laboratory, Faculty of Science, Charles University, Prague). .. Expression vector pET-22b(+) containing the EnSerp1 insert (1200 bp) was synthesised by GeneScript company, with the coding sequence for His-tag on C-terminus.

    Article Title: Compound heterozygous variants in the multiple PDZ domain protein (MPDZ) cause a case of mild non-progressive communicating hydrocephalus
    Article Snippet: To demonstrate the splice-effect of the identified variant (c.394G > A) on the mRNA level, exon 5 and parts of its flanking introns were sub-cloned into the mini-cassette vector using BSTEII ( GGTCACC) and NheI ( GCTAGC) restriction enzymes (New England Biolabs), they were included in the following cloning primers: Forward primer: 5’-ATGTG GGTCACC ACACTTACTAAG-3′, Reverse primer: 5′- TATTATGGGTCATT GCTAGC ATGG-3′. .. After amplifying the desired genomic fragment of MPDZ gene which was around 647 bps, it was further purified using MinElute PCR Purification Kit (Qiagen.

    Article Title: Biosynthetically-Intriguing Chlorinated Lipophilic Metabolites from Geographically Distant Tropical Marine Cyanobacteria
    Article Snippet: PCR products were purified using a MinElute® PCR Purification Kit (Qiagen) before subcloning using the Zero Blunt® TOPO® PCR Cloning Kit (Invitrogen) following the manufacturer’s specifications. .. Plasmid DNA was isolated using the QIAprep® Spin Miniprep Kit (Qiagen) and sequenced with M13 primers.

    Enzyme-linked Immunosorbent Assay:

    Article Title: An HIV-Encoded Antisense Long Noncoding RNA Epigenetically Regulates Viral Transcription
    Article Snippet: Viral p24 analysis was performed by the Translational Virology Core at the University of California San Diego Center for AIDS Research using the PerkinElmer Extended Curve p24 enzyme-linked immunosorbent assay. .. ChIP eluates were purified using the MinElute PCR purification kit (Qiagen) and analyzed by qPCR.

    Positron Emission Tomography:

    Article Title: Identification and partial characterization of a novel serpin from Eudiplozoon nipponicum (Monogenea, Polyopisthocotylea)
    Article Snippet: The PCR products (1200 bp) were resolved in 1% agarose gel, purified by a MinElute PCR Purification Kit (Qiagen) and sub-cloned into a pJET1.2 cloning vector (CloneJET PCR Cloning Kit, Thermo Scientific), which was used to transform E. coli XL-1 Blue (Novagen). pJET1.2 constructs were isolated using a High Pure Plasmid Isolation Kit (Roche) and sequenced with pJET1.2 forward and reverse primers from the kit (DNA Sequencing Laboratory, Faculty of Science, Charles University, Prague). .. Expression vector pET-22b(+) containing the EnSerp1 insert (1200 bp) was synthesised by GeneScript company, with the coding sequence for His-tag on C-terminus.

    Sample Prep:

    Article Title: A phylogenetic framework facilitates Y-STR variant discovery and classification via massively parallel sequencing
    Article Snippet: Amplified products were purified using the MinElute® PCR purification kit (Qiagen), then quantified using the Qubit® dsDNA BR kit on the Qubit® 2.0 fluorometer. .. Library preparation was performed on ∼500 ng product per sample using the TruSeq® DNA PCR-free LT (24-plex) and HT (96-plex) sample preparation reagents (Illumina).

    Article Title: Assessment of the gorilla gut virome in association with natural simian immunodeficiency virus infection
    Article Snippet: The MinElute PCR Purification Kit (QIAGEN) and the Agencourt AMPure XP System: PCR Purification kit (Beckman Coulter, CA, USA) were used to purify PCR-amplified products whenever requested by the ScriptSeq™ kit protocol. .. DNA libraries were constructed using the Nextera® DNA Sample Preparation kit (Illumina® , CA, USA), following the manufacturer’s instructions.

    Article Title: CRISPR-Cap: multiplexed double-stranded DNA enrichment based on the CRISPR system
    Article Snippet: We incubated the solution containing the released DNA for 5 min at room temperature and then purified the DNA using the MinElute PCR Purification Kit (QIAGEN) to remove any remaining SDS. .. We used the column-purified DNA or heat-released DNA for NGS sample preparation.

    Size-exclusion Chromatography:

    Article Title: Biosynthetically-Intriguing Chlorinated Lipophilic Metabolites from Geographically Distant Tropical Marine Cyanobacteria
    Article Snippet: The PCR reactions were performed using the following gradient: initial denaturation for 2 min at 95°C, 25 cycles of amplification, followed by 20 sec at 95°C, 20 sec at 55°C and 1.5 min at 72 °C, and final elongation for 3 min at 72°C. .. PCR products were purified using a MinElute® PCR Purification Kit (Qiagen) before subcloning using the Zero Blunt® TOPO® PCR Cloning Kit (Invitrogen) following the manufacturer’s specifications.

    Next-Generation Sequencing:

    Article Title: Pseudomonas-Specific NGS Assay Provides Insight Into Abundance and Dynamics of Pseudomonas Species Including P. aeruginosa in a Cooling Tower
    Article Snippet: Paragraph title: Design of Pseudomonas NGS Libraries ... Subsequently, pooled libraries were purified by the MinElute PCR Purification Kit (Qiagen, Hilden, Germany).

    Article Title: Assessment of the gorilla gut virome in association with natural simian immunodeficiency virus infection
    Article Snippet: Paragraph title: Library preparation, quantification and NGS ... The MinElute PCR Purification Kit (QIAGEN) and the Agencourt AMPure XP System: PCR Purification kit (Beckman Coulter, CA, USA) were used to purify PCR-amplified products whenever requested by the ScriptSeq™ kit protocol.

    Article Title: CRISPR-Cap: multiplexed double-stranded DNA enrichment based on the CRISPR system
    Article Snippet: We incubated the solution containing the released DNA for 5 min at room temperature and then purified the DNA using the MinElute PCR Purification Kit (QIAGEN) to remove any remaining SDS. .. We used the column-purified DNA or heat-released DNA for NGS sample preparation.

    Spectrophotometry:

    Article Title: First record of the nematode Libyostrongylus dentatus Hoberg, Lloyd Omar, 1995 (Trichostrongylidae) in ostriches (Struthio camelus Linnaeus, 1758) (Struthionidae) outside the Americas
    Article Snippet: The DNA was quantified in a ND-1000 NanoDrop Spectrophotometer (Thermo Fisher Scientific, Wilmington, USA). .. For PCR product purification, a MinElute PCR purification kit (Qiagen) was used according to the manufacturer’s protocol.

    DNA Extraction:

    Article Title: First record of the nematode Libyostrongylus dentatus Hoberg, Lloyd Omar, 1995 (Trichostrongylidae) in ostriches (Struthio camelus Linnaeus, 1758) (Struthionidae) outside the Americas
    Article Snippet: Paragraph title: DNA extraction, PCR amplification and sequencing ... For PCR product purification, a MinElute PCR purification kit (Qiagen) was used according to the manufacturer’s protocol.

    Article Title: Biosynthetically-Intriguing Chlorinated Lipophilic Metabolites from Geographically Distant Tropical Marine Cyanobacteria
    Article Snippet: Paragraph title: DNA Extraction, PCR-amplification, and Cloning ... PCR products were purified using a MinElute® PCR Purification Kit (Qiagen) before subcloning using the Zero Blunt® TOPO® PCR Cloning Kit (Invitrogen) following the manufacturer’s specifications.

    Immunoprecipitation:

    Article Title: An HIV-Encoded Antisense Long Noncoding RNA Epigenetically Regulates Viral Transcription
    Article Snippet: ChIP analyses and RNA immunoprecipitation (RNA-IP) analyses were performed by standard protocols adapted for automation using the epMotion 5070 automated pipetting system (Eppendorf, Hamburg, Germany). .. ChIP eluates were purified using the MinElute PCR purification kit (Qiagen) and analyzed by qPCR.

    Article Title: DNMT3A and TET1 cooperate to regulate promoter epigenetic landscapes in mouse embryonic stem cells
    Article Snippet: .. Then cross-linking was reversed at 68 °C and immunoprecipitated DNA was purified by MinElute PCR purification Kit (Qiagen). .. H3K4me3 (Millipore, 07–473) and H3K27me3 (Millipore, 07–449; Cell signaling, 9733S) ChIP were performed as previously described [ ], with 3 million cells.

    DNA Purification:

    Article Title: Biosynthetically-Intriguing Chlorinated Lipophilic Metabolites from Geographically Distant Tropical Marine Cyanobacteria
    Article Snippet: The biomass was pretreated using TE (10 mM Tris; 0.1M EDTA; 0.5 % SDS; 20 µg/mL RNase)/lysozyme (1 mg/mL) at 37°C for 30 min followed by incubation with proteinase K (0.5 mg/mL) at 50°C for 1 h. Genomic DNA was extracted using the Wizard® Genomic DNA Purification Kit (Promega) following the manufacturer’s specifications. .. PCR products were purified using a MinElute® PCR Purification Kit (Qiagen) before subcloning using the Zero Blunt® TOPO® PCR Cloning Kit (Invitrogen) following the manufacturer’s specifications.

    Lysis:

    Article Title: Interleukin 2 modulates thymic-derived regulatory T cell epigenetic landscape
    Article Snippet: Sorted cells were next cross-linked in 1% (wt/vol) formaldehyde solution for 30 min. Cross-linked DNA was lysed in Farnham lysis buffer (5 mM PIPES pH 8.0, 85 mM KCl and 0.5% NP-40), fragmented in RIPA buffer (1% NP-40, 0.5% sodium deoxycholate, and 0.1% SDS in 1×PBS) using Bioruptor (Diagenode), and incubated overnight at 4 °C with 100 µL of DynaBeads M-280 Sheep anti-Rabbit IgG magnet beads (Invitrogen) preincubated with 20 µg of anti-SATB1 antibody (Abcam, ab70004). .. SATB1-bound DNA was then eluted from the beads and reverse cross-linked by incubating the beads pellet in 200 µl of IP elution buffer (1% SDS and 0.1 M NaHCO3 ) at 65 °C overnight and further purified with MinElute PCR Purification Kit (QIAGEN).

    Article Title: An HIV-Encoded Antisense Long Noncoding RNA Epigenetically Regulates Viral Transcription
    Article Snippet: Samples were neutralized by lysis in Triton X-100 at a final concentration of 0.5%. .. ChIP eluates were purified using the MinElute PCR purification kit (Qiagen) and analyzed by qPCR.

    Article Title: Disruption of mesoderm formation during cardiac differentiation due to developmental exposure to 13-cis-retinoic acid
    Article Snippet: Cell pellets were re-suspended in 25 μl of ice-cold lysis buffer (10 mM Tris-HCl, 10 mM NaCl, 3 mM MgCl2 , and 0.1% Igepal CA-630, pH 7.4), and nuclei were pelleted by centrifugation for 30 min at 500 g and 4 °C. .. The reaction was incubated at 37 °C for 30 min, and subsequently the reaction mixture was purified using MinElute PCR Purification Kit (Qiagen).

    Staining:

    Article Title: Interleukin 2 modulates thymic-derived regulatory T cell epigenetic landscape
    Article Snippet: After staining the remaining cells with fluorescent labeled antibodies against CD4 (GK1.5) and CD8α (53-6.7), we flow-sorted 2 × 106 CD4+ CD8αneg SP thymocytes. .. SATB1-bound DNA was then eluted from the beads and reverse cross-linked by incubating the beads pellet in 200 µl of IP elution buffer (1% SDS and 0.1 M NaHCO3 ) at 65 °C overnight and further purified with MinElute PCR Purification Kit (QIAGEN).

    Article Title: Pseudomonas-Specific NGS Assay Provides Insight Into Abundance and Dynamics of Pseudomonas Species Including P. aeruginosa in a Cooling Tower
    Article Snippet: After the second PCR amplification, the libraries were size-selected by gel electrophoresis on a 2% agarose gel pre-stained with GelRed Nucleic Acid Gel Stain (Biotium, Hayward, CA, United States), and recovered using a slightly modified QIAquick Gel Extraction Kit (Qiagen, Hilden, Germany) protocol ( ). .. Subsequently, pooled libraries were purified by the MinElute PCR Purification Kit (Qiagen, Hilden, Germany).

    Article Title: First record of the nematode Libyostrongylus dentatus Hoberg, Lloyd Omar, 1995 (Trichostrongylidae) in ostriches (Struthio camelus Linnaeus, 1758) (Struthionidae) outside the Americas
    Article Snippet: PCR products were electrophoresed on 2.0% agarose gels and visualized using GelRed nucleic acid gel stain (Biotium, Hayward, USA). .. For PCR product purification, a MinElute PCR purification kit (Qiagen) was used according to the manufacturer’s protocol.

    Variant Assay:

    Article Title: Compound heterozygous variants in the multiple PDZ domain protein (MPDZ) cause a case of mild non-progressive communicating hydrocephalus
    Article Snippet: To demonstrate the splice-effect of the identified variant (c.394G > A) on the mRNA level, exon 5 and parts of its flanking introns were sub-cloned into the mini-cassette vector using BSTEII ( GGTCACC) and NheI ( GCTAGC) restriction enzymes (New England Biolabs), they were included in the following cloning primers: Forward primer: 5’-ATGTG GGTCACC ACACTTACTAAG-3′, Reverse primer: 5′- TATTATGGGTCATT GCTAGC ATGG-3′. .. After amplifying the desired genomic fragment of MPDZ gene which was around 647 bps, it was further purified using MinElute PCR Purification Kit (Qiagen.

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    Qiagen minelute pcr purification kit
    DNA purification reagents vary in their ability to recover low amounts of DNA from de-crosslinked chromatin. a Recovered DNA amount by different DNA purification reagents from de-crosslinked chromatin. De-crosslinked chromatin estimated to include 1 ng range DNA in ChIP elution buffer was purified following the manufacturer’s instructions. The data were generated from triplicate DNA samples derived from three independent preparations. Zy, ChIP DNA Clean Concentrator™ (Zymo Research); Pr, Wizard® SV Gel and <t>PCR</t> Clean-Up System (Promega); Th, GeneJET PCR Purification Kit (Thermo Fisher Scientific); In, PureLink® PCR Purification Kit (Invitrogen); Ne, Monarch® PCR DNA Cleanup Kit (New England Biolabs); Am, Chromatin IP DNA Purification Kit (Active Motif); Qp, QIAquick PCR Purification Kit (Qiagen); Qm, <t>MinElute</t> PCR Purification Kit (Qiagen); Ba, Agencourt AMPure XP kit (Beckman, chromatin to beads ratio from 1:1.25 to 1:2); Br, RNAClean™ XP kit (Beckman, chromatin to beads ratio from 1:1.25 to 1:2); PC, phenol/chloroform extraction. b Interference of PCR amplification by purified eluent of purification reagents. 9 μL eluent was mixed with 1 μL 166 bp of Drosophila probe DNA (0.0001 ng), and the resulting mixture was used as the template in 20 μl of real-time PCR reaction. The Ct value for Drosophila probe DNA from TE buffer was set as 100%. The experiment was repeated 3 times using de-crosslinked chromatin estimated to include 1 ng of DNA. c Size profiles of DNA purified by different reagents. The DNAs purified from de-crosslinked chromatin estimated to include 50 ng range DNA was analyzed by AATI Fragment Analyzer. DNA size (bp) is shown
    Minelute Pcr Purification Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 95/100, based on 2289 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    DNA purification reagents vary in their ability to recover low amounts of DNA from de-crosslinked chromatin. a Recovered DNA amount by different DNA purification reagents from de-crosslinked chromatin. De-crosslinked chromatin estimated to include 1 ng range DNA in ChIP elution buffer was purified following the manufacturer’s instructions. The data were generated from triplicate DNA samples derived from three independent preparations. Zy, ChIP DNA Clean Concentrator™ (Zymo Research); Pr, Wizard® SV Gel and PCR Clean-Up System (Promega); Th, GeneJET PCR Purification Kit (Thermo Fisher Scientific); In, PureLink® PCR Purification Kit (Invitrogen); Ne, Monarch® PCR DNA Cleanup Kit (New England Biolabs); Am, Chromatin IP DNA Purification Kit (Active Motif); Qp, QIAquick PCR Purification Kit (Qiagen); Qm, MinElute PCR Purification Kit (Qiagen); Ba, Agencourt AMPure XP kit (Beckman, chromatin to beads ratio from 1:1.25 to 1:2); Br, RNAClean™ XP kit (Beckman, chromatin to beads ratio from 1:1.25 to 1:2); PC, phenol/chloroform extraction. b Interference of PCR amplification by purified eluent of purification reagents. 9 μL eluent was mixed with 1 μL 166 bp of Drosophila probe DNA (0.0001 ng), and the resulting mixture was used as the template in 20 μl of real-time PCR reaction. The Ct value for Drosophila probe DNA from TE buffer was set as 100%. The experiment was repeated 3 times using de-crosslinked chromatin estimated to include 1 ng of DNA. c Size profiles of DNA purified by different reagents. The DNAs purified from de-crosslinked chromatin estimated to include 50 ng range DNA was analyzed by AATI Fragment Analyzer. DNA size (bp) is shown

    Journal: BMC Genomics

    Article Title: Purification of nanogram-range immunoprecipitated DNA in ChIP-seq application

    doi: 10.1186/s12864-017-4371-5

    Figure Lengend Snippet: DNA purification reagents vary in their ability to recover low amounts of DNA from de-crosslinked chromatin. a Recovered DNA amount by different DNA purification reagents from de-crosslinked chromatin. De-crosslinked chromatin estimated to include 1 ng range DNA in ChIP elution buffer was purified following the manufacturer’s instructions. The data were generated from triplicate DNA samples derived from three independent preparations. Zy, ChIP DNA Clean Concentrator™ (Zymo Research); Pr, Wizard® SV Gel and PCR Clean-Up System (Promega); Th, GeneJET PCR Purification Kit (Thermo Fisher Scientific); In, PureLink® PCR Purification Kit (Invitrogen); Ne, Monarch® PCR DNA Cleanup Kit (New England Biolabs); Am, Chromatin IP DNA Purification Kit (Active Motif); Qp, QIAquick PCR Purification Kit (Qiagen); Qm, MinElute PCR Purification Kit (Qiagen); Ba, Agencourt AMPure XP kit (Beckman, chromatin to beads ratio from 1:1.25 to 1:2); Br, RNAClean™ XP kit (Beckman, chromatin to beads ratio from 1:1.25 to 1:2); PC, phenol/chloroform extraction. b Interference of PCR amplification by purified eluent of purification reagents. 9 μL eluent was mixed with 1 μL 166 bp of Drosophila probe DNA (0.0001 ng), and the resulting mixture was used as the template in 20 μl of real-time PCR reaction. The Ct value for Drosophila probe DNA from TE buffer was set as 100%. The experiment was repeated 3 times using de-crosslinked chromatin estimated to include 1 ng of DNA. c Size profiles of DNA purified by different reagents. The DNAs purified from de-crosslinked chromatin estimated to include 50 ng range DNA was analyzed by AATI Fragment Analyzer. DNA size (bp) is shown

    Article Snippet: The ChIP DNA Clean & Concentrator™ (Zymo Research; Zy), the Monarch® PCR & DNA Cleanup Kit (New England Biolabs; Ne), the MinElute PCR Purification Kit (Qiagen, Qm), the QIAquick PCR Purification Kit (Qiagen; Qp), the Agencourt AMPure XP kit (Beckman; Ba) and the RNAClean™ XP kit (Beckman; Br), and phenol/chloroform extraction (Invitrogen; PC) performed well with de-crosslinked chromatin.

    Techniques: DNA Purification, Chromatin Immunoprecipitation, Purification, Generated, Derivative Assay, Polymerase Chain Reaction, Amplification, Real-time Polymerase Chain Reaction