minelute qiagen pcr purification kit  (Qiagen)


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    Name:
    MinElute PCR Purification Kit
    Description:
    For purification of up to 5 μg PCR products 70 bp to 4 kb in low elution volumes Kit contents Qiagen MinElute PCR Purification Kit 50 MinElute Spin Columns 5g Binding Capacity 10L Elution Volume Tube Format Silica Technology 70 bp to 4 kb Fragment Size Manual Processing DNA Sample Fast Procedure and Easy Handling High Reproducible Recoveries For Purification of up to 5μg PCR Products in Low Elution Volumes Includes 50 MinElute Spin Columns Buffers 2mL Collection Tubes Benefits Very small elution volumes Fast procedure and easy handling High reproducible recoveries Gel loading dye for convenient sample analysis
    Catalog Number:
    28004
    Price:
    134
    Category:
    MinElute PCR Purification Kit
    Buy from Supplier


    Structured Review

    Qiagen minelute qiagen pcr purification kit
    MinElute PCR Purification Kit
    For purification of up to 5 μg PCR products 70 bp to 4 kb in low elution volumes Kit contents Qiagen MinElute PCR Purification Kit 50 MinElute Spin Columns 5g Binding Capacity 10L Elution Volume Tube Format Silica Technology 70 bp to 4 kb Fragment Size Manual Processing DNA Sample Fast Procedure and Easy Handling High Reproducible Recoveries For Purification of up to 5μg PCR Products in Low Elution Volumes Includes 50 MinElute Spin Columns Buffers 2mL Collection Tubes Benefits Very small elution volumes Fast procedure and easy handling High reproducible recoveries Gel loading dye for convenient sample analysis
    https://www.bioz.com/result/minelute qiagen pcr purification kit/product/Qiagen
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    minelute qiagen pcr purification kit - by Bioz Stars, 2020-04
    99/100 stars

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    Related Articles

    Amplification:

    Article Title: Protocol for Metagenomic Virus Detection in Clinical Specimens 1
    Article Snippet: Second-strand synthesis was performed by using the NEBNext mRNA Second Strand Synthesis Module (New England Biolabs) and purified by using the MinElute PCR Purification Kit (QIAGEN). .. The sequencing library was then amplified by using an emulsion-based clonal amplification PCR in the Ion OneTouch 200 Template v2 DL Kit and enriched by using an Ion OneTouch Enrichment System.

    Article Title: Dysregulated immune system networks in war veterans with PTSD is an outcome of altered miRNA expression and DNA methylation
    Article Snippet: After DNA fragments were purified using Qiagen PCR purification kit (Qiagen catalogue #28004), an “A” base was added to the 3′ end of the blunt DNA fragment by Klenow fragment. .. The libraries were then amplified by limited PCR (15 cycles) using primers provided by the kit.

    Real-time Polymerase Chain Reaction:

    Article Title: Purification of nanogram-range immunoprecipitated DNA in ChIP-seq application
    Article Snippet: The ChIP DNA Clean & Concentrator™ (Zymo Research; Zy), the Monarch® PCR & DNA Cleanup Kit (New England Biolabs; Ne), the MinElute PCR Purification Kit (Qiagen, Qm), the QIAquick PCR Purification Kit (Qiagen; Qp), the Agencourt AMPure XP kit (Beckman; Ba) and the RNAClean™ XP kit (Beckman; Br), and phenol/chloroform extraction (Invitrogen; PC) performed well with de-crosslinked chromatin. .. We utilized qPCR approach to check the potential interference of each purification reagent in the downstream application.

    Incubation:

    Article Title: Purification of nanogram-range immunoprecipitated DNA in ChIP-seq application
    Article Snippet: After adding 30 μl of prewashed protein G-magnetic beads, the reaction was further incubated for 3 h. The beads were extensively washed with ChIP buffer, high salt buffer, LiCl2 buffer, and TE buffer. .. After treatment with RNase A and proteinase K, DNA was purified by Qiagen MinElute PCR Purification Kit (Cat. # 28006, Valencia, CA) and quantified using Qubit dsDNA High Sensitivity assay (Invitrogen, Q32851).

    Expressing:

    Article Title: Components of the interleukin-33/ST2 system are differentially expressed and regulated in human cardiac cells and in cells of the cardiac vasculature
    Article Snippet: 2.4 Total RNA purification and cDNA preparation Cells were treated as described (for IL-33 mRNA determination) or left untreated (for measurement of basal total ST2, ST2L, or sST2 mRNA expression), supernatants were removed and total cellular RNA was isolated using High Pure RNA Isolation Kit (Roche, Basel, Switzerland) according to the manufacturer's instructions. .. For PCR of ST2 isoforms, cDNA was additionally eluted with MinElute PCR Purification Kit (QIAGEN GmbH, Hilden, Germany).

    Derivative Assay:

    Article Title: Purification of nanogram-range immunoprecipitated DNA in ChIP-seq application
    Article Snippet: Similarly, DNAs were purified from de-crosslinked chromatin estimated to include 1 ng, 5 ng, 10 ng, and 50 ng of DNA after treatment of RNase A and proteinase K. The following reagents were used in the experiment: ChIP DNA Clean & Concentrator™ (Cat. # D5205) from Zymo Research (Zy) (Irvine, CA); Wizard® SV Gel and PCR Clean-Up System (Cat. # A9281) from Promega (Pr) (Fitchburg, WI); GeneJET PCR Purification Kit (Cat. # K0701) from Thermo Fisher Scientific (Th) (Waltham, MA); PureLink® PCR Purification Kit (Cat. # K310001) from Invitrogen (In) (Carlsbad, CA); Monarch® PCR & DNA Cleanup Kit (Cat. # T1030S) from New England Biolabs (Ne) (Ipswich, MA); Chromatin IP DNA Purification Kit (Cat. # 58002) from Active Motif (Am) (Carlsbad, CA); QIAquick PCR Purification Kit (Cat. # 28106) from Qiagen (Qp) (Valencia, CA), MinElute PCR Purification Kit (Cat. # 28006) from Qiagen (Qm); Agencourt AMPure XP (Cat. # A63881) from Beckman (Ba) (Indianapolis, IN), RNAClean™ XP (Cat. # A63987) from Beckman (Br), and phenol/chloroform extraction (PC) (Additional file ). .. Each purification reagent was tested in triplicate DNA samples derived from 3 independent experiments.

    Generated:

    Article Title: Purification of nanogram-range immunoprecipitated DNA in ChIP-seq application
    Article Snippet: The anti-H3K27me3 antibody (Cat. #9733, Lot #8) was purchased from Cell Signaling Technology (Danvers, MA) and the purified anti-H3K4me3 antibody was generated in-house (EDL Lot 1). .. After treatment with RNase A and proteinase K, DNA was purified by Qiagen MinElute PCR Purification Kit (Cat. # 28006, Valencia, CA) and quantified using Qubit dsDNA High Sensitivity assay (Invitrogen, Q32851).

    Polymerase Chain Reaction:

    Article Title: Purification of nanogram-range immunoprecipitated DNA in ChIP-seq application
    Article Snippet: .. Similarly, DNAs were purified from de-crosslinked chromatin estimated to include 1 ng, 5 ng, 10 ng, and 50 ng of DNA after treatment of RNase A and proteinase K. The following reagents were used in the experiment: ChIP DNA Clean & Concentrator™ (Cat. # D5205) from Zymo Research (Zy) (Irvine, CA); Wizard® SV Gel and PCR Clean-Up System (Cat. # A9281) from Promega (Pr) (Fitchburg, WI); GeneJET PCR Purification Kit (Cat. # K0701) from Thermo Fisher Scientific (Th) (Waltham, MA); PureLink® PCR Purification Kit (Cat. # K310001) from Invitrogen (In) (Carlsbad, CA); Monarch® PCR & DNA Cleanup Kit (Cat. # T1030S) from New England Biolabs (Ne) (Ipswich, MA); Chromatin IP DNA Purification Kit (Cat. # 58002) from Active Motif (Am) (Carlsbad, CA); QIAquick PCR Purification Kit (Cat. # 28106) from Qiagen (Qp) (Valencia, CA), MinElute PCR Purification Kit (Cat. # 28006) from Qiagen (Qm); Agencourt AMPure XP (Cat. # A63881) from Beckman (Ba) (Indianapolis, IN), RNAClean™ XP (Cat. # A63987) from Beckman (Br), and phenol/chloroform extraction (PC) (Additional file ). ..

    Article Title: Purification of nanogram-range immunoprecipitated DNA in ChIP-seq application
    Article Snippet: .. Lane 1 (EDL St), the library from 1 ng of purified ChIP DNA from aliquot A; Lane 2 (1 ng QmM), the library from stored DNA in MaxyClear tube after purification by MinElute PCR Purification Kit from aliquot B; Lane 3 (1 ng QmP), the library from stored DNA in Premium Tube after purification by MinElute PCR Purification Kit from aliquot B; Lane 4 (Input), the library from 1 ng of purified input DNA from the aliquot A. .. Left panel is the profile of H3K4me3 libraries, and right panel is the profile of H3K27me3 libraries. (PDF 123 kb) Mapping results of ChIP-seq reads generated through different purification reagents. (PDF 94 kb) Measuring global ChIP enrichment by FRiP (fraction of reads in peaks).

    Article Title: Purification of nanogram-range immunoprecipitated DNA in ChIP-seq application
    Article Snippet: .. DNA was purified using MinElute PCR Purification Kit after treatment of RNase A and proteinase K. DNA was quantified using Qubit dsDNA High Sensitivity assay and adjusted to 1 ng/μL with TE buffer. .. DNAs were prepared to final 1 ng, 5 ng, 10 ng and 50 ng in 100 μL ChIP elution buffer and were purified by 11 different purification reagents as suggested by the manufacturer except for the elution volume, which was fixed at 16 μL.

    Article Title: Purification of nanogram-range immunoprecipitated DNA in ChIP-seq application
    Article Snippet: .. The ChIP DNA Clean & Concentrator™ (Zymo Research; Zy), the Monarch® PCR & DNA Cleanup Kit (New England Biolabs; Ne), the MinElute PCR Purification Kit (Qiagen, Qm), the QIAquick PCR Purification Kit (Qiagen; Qp), the Agencourt AMPure XP kit (Beckman; Ba) and the RNAClean™ XP kit (Beckman; Br), and phenol/chloroform extraction (Invitrogen; PC) performed well with de-crosslinked chromatin. .. These reagents recovered 78.1% to 95.7% with 10–50 ng of purified DNA, 81.7% to 96.8% with 5 ng of DNA, and 68.1% to 82.9% with 1 ng of DNA except phenol/chloroform extraction with over 100%.

    Article Title: A retrospective study of community-acquired Clostridium difficile infection in southwest China
    Article Snippet: .. PCR ribotyping reaction products were concentrated using a Qiagen Min-Elute PCR purification kit (QIAGEN) and run on a QIAxcel capillary electrophoresis platform (QIAGEN). .. Visualization of PCR products was performed with QIAxcel ScreenGel software (v1.3.0; QIAGEN).

    Article Title: Purification of nanogram-range immunoprecipitated DNA in ChIP-seq application
    Article Snippet: .. For the MinElute PCR Purification Kit, DNA was eluted in Maxyclear and Premium tubes. ..

    Article Title: Purification of nanogram-range immunoprecipitated DNA in ChIP-seq application
    Article Snippet: .. Aliquot A was purified by the lab standard protocol using MinElute PCR Purification Kit in a MaxyClear tube. .. The ChIP enrichment was analyzed by quantitative real-time PCR in positive and negative genomic loci (Additional file ).

    Article Title: Components of the interleukin-33/ST2 system are differentially expressed and regulated in human cardiac cells and in cells of the cardiac vasculature
    Article Snippet: .. For PCR of ST2 isoforms, cDNA was additionally eluted with MinElute PCR Purification Kit (QIAGEN GmbH, Hilden, Germany). .. 2.5 RealTime-PCR RealTime-PCR was performed using LightCycler® TaqMan® Master (Roche) according to the manufacturer's instructions.

    Article Title: Protocol for Metagenomic Virus Detection in Clinical Specimens 1
    Article Snippet: .. Second-strand synthesis was performed by using the NEBNext mRNA Second Strand Synthesis Module (New England Biolabs) and purified by using the MinElute PCR Purification Kit (QIAGEN). .. Double-stranded cDNA, DNA, and random PCR products were quantified by using the Qubit HS dsDNA Kit (Invitrogen Life Technologies).

    Article Title: Purification of nanogram-range immunoprecipitated DNA in ChIP-seq application
    Article Snippet: .. After treatment with RNase A and proteinase K, DNA was purified by Qiagen MinElute PCR Purification Kit (Cat. # 28006, Valencia, CA) and quantified using Qubit dsDNA High Sensitivity assay (Invitrogen, Q32851). .. To check the size of input chromatin, purified input DNA was analyzed by Fragment Analyzer (Advanced Analytical Technologies; AATI; Ankeny, IA) using the High Sensitivity NGS Fragment Analysis Kit (Cat. # DNF-486).

    Article Title: Dysregulated immune system networks in war veterans with PTSD is an outcome of altered miRNA expression and DNA methylation
    Article Snippet: .. After DNA fragments were purified using Qiagen PCR purification kit (Qiagen catalogue #28004), an “A” base was added to the 3′ end of the blunt DNA fragment by Klenow fragment. .. Sequencing adapters were ligated to the ends of DNA fragments using DNA ligase.

    Sonication:

    Article Title: Purification of nanogram-range immunoprecipitated DNA in ChIP-seq application
    Article Snippet: After adding the same volume of sonication buffer (100 mM Tris-HCl, pH 8.1, 20 mM EDTA, 200 mM NaCl, 2% Triton X-100, 0.2% sodium deoxycholate), the lysate was sonicated for 15 cycles (30 s on, 30 s off) using a Diagenode Bioruptor and centrifuged at 15,000 rpm for 10 min. .. After treatment with RNase A and proteinase K, DNA was purified by Qiagen MinElute PCR Purification Kit (Cat. # 28006, Valencia, CA) and quantified using Qubit dsDNA High Sensitivity assay (Invitrogen, Q32851).

    DNA Extraction:

    Article Title: A retrospective study of community-acquired Clostridium difficile infection in southwest China
    Article Snippet: Paragraph title: Bacterial DNA isolation, PCR-ribotyping and toxin gene profiling ... PCR ribotyping reaction products were concentrated using a Qiagen Min-Elute PCR purification kit (QIAGEN) and run on a QIAxcel capillary electrophoresis platform (QIAGEN).

    RNA Sequencing Assay:

    Article Title: Dysregulated immune system networks in war veterans with PTSD is an outcome of altered miRNA expression and DNA methylation
    Article Snippet: Paragraph title: RNA-sequencing (RNA-Seq) ... After DNA fragments were purified using Qiagen PCR purification kit (Qiagen catalogue #28004), an “A” base was added to the 3′ end of the blunt DNA fragment by Klenow fragment.

    Sensitive Assay:

    Article Title: Purification of nanogram-range immunoprecipitated DNA in ChIP-seq application
    Article Snippet: DNA was purified using MinElute PCR Purification Kit after treatment of RNase A and proteinase K. DNA was quantified using Qubit dsDNA High Sensitivity assay and adjusted to 1 ng/μL with TE buffer. .. Similarly, DNAs were purified from de-crosslinked chromatin estimated to include 1 ng, 5 ng, 10 ng, and 50 ng of DNA after treatment of RNase A and proteinase K. The following reagents were used in the experiment: ChIP DNA Clean & Concentrator™ (Cat. # D5205) from Zymo Research (Zy) (Irvine, CA); Wizard® SV Gel and PCR Clean-Up System (Cat. # A9281) from Promega (Pr) (Fitchburg, WI); GeneJET PCR Purification Kit (Cat. # K0701) from Thermo Fisher Scientific (Th) (Waltham, MA); PureLink® PCR Purification Kit (Cat. # K310001) from Invitrogen (In) (Carlsbad, CA); Monarch® PCR & DNA Cleanup Kit (Cat. # T1030S) from New England Biolabs (Ne) (Ipswich, MA); Chromatin IP DNA Purification Kit (Cat. # 58002) from Active Motif (Am) (Carlsbad, CA); QIAquick PCR Purification Kit (Cat. # 28106) from Qiagen (Qp) (Valencia, CA), MinElute PCR Purification Kit (Cat. # 28006) from Qiagen (Qm); Agencourt AMPure XP (Cat. # A63881) from Beckman (Ba) (Indianapolis, IN), RNAClean™ XP (Cat. # A63987) from Beckman (Br), and phenol/chloroform extraction (PC) (Additional file ).

    Article Title: Purification of nanogram-range immunoprecipitated DNA in ChIP-seq application
    Article Snippet: .. DNA was purified using MinElute PCR Purification Kit after treatment of RNase A and proteinase K. DNA was quantified using Qubit dsDNA High Sensitivity assay and adjusted to 1 ng/μL with TE buffer. .. DNAs were prepared to final 1 ng, 5 ng, 10 ng and 50 ng in 100 μL ChIP elution buffer and were purified by 11 different purification reagents as suggested by the manufacturer except for the elution volume, which was fixed at 16 μL.

    Article Title: Purification of nanogram-range immunoprecipitated DNA in ChIP-seq application
    Article Snippet: .. After treatment with RNase A and proteinase K, DNA was purified by Qiagen MinElute PCR Purification Kit (Cat. # 28006, Valencia, CA) and quantified using Qubit dsDNA High Sensitivity assay (Invitrogen, Q32851). .. To check the size of input chromatin, purified input DNA was analyzed by Fragment Analyzer (Advanced Analytical Technologies; AATI; Ankeny, IA) using the High Sensitivity NGS Fragment Analysis Kit (Cat. # DNF-486).

    Isolation:

    Article Title: Components of the interleukin-33/ST2 system are differentially expressed and regulated in human cardiac cells and in cells of the cardiac vasculature
    Article Snippet: Frozen human myocardial tissue was homogenized using a ball mill (Retsch, Haan, Germany), and mRNA was isolated using High Pure RNA Tissue Kit (Roche). .. For PCR of ST2 isoforms, cDNA was additionally eluted with MinElute PCR Purification Kit (QIAGEN GmbH, Hilden, Germany).

    Purification:

    Article Title: Purification of nanogram-range immunoprecipitated DNA in ChIP-seq application
    Article Snippet: .. Similarly, DNAs were purified from de-crosslinked chromatin estimated to include 1 ng, 5 ng, 10 ng, and 50 ng of DNA after treatment of RNase A and proteinase K. The following reagents were used in the experiment: ChIP DNA Clean & Concentrator™ (Cat. # D5205) from Zymo Research (Zy) (Irvine, CA); Wizard® SV Gel and PCR Clean-Up System (Cat. # A9281) from Promega (Pr) (Fitchburg, WI); GeneJET PCR Purification Kit (Cat. # K0701) from Thermo Fisher Scientific (Th) (Waltham, MA); PureLink® PCR Purification Kit (Cat. # K310001) from Invitrogen (In) (Carlsbad, CA); Monarch® PCR & DNA Cleanup Kit (Cat. # T1030S) from New England Biolabs (Ne) (Ipswich, MA); Chromatin IP DNA Purification Kit (Cat. # 58002) from Active Motif (Am) (Carlsbad, CA); QIAquick PCR Purification Kit (Cat. # 28106) from Qiagen (Qp) (Valencia, CA), MinElute PCR Purification Kit (Cat. # 28006) from Qiagen (Qm); Agencourt AMPure XP (Cat. # A63881) from Beckman (Ba) (Indianapolis, IN), RNAClean™ XP (Cat. # A63987) from Beckman (Br), and phenol/chloroform extraction (PC) (Additional file ). ..

    Article Title: Purification of nanogram-range immunoprecipitated DNA in ChIP-seq application
    Article Snippet: .. Lane 1 (EDL St), the library from 1 ng of purified ChIP DNA from aliquot A; Lane 2 (1 ng QmM), the library from stored DNA in MaxyClear tube after purification by MinElute PCR Purification Kit from aliquot B; Lane 3 (1 ng QmP), the library from stored DNA in Premium Tube after purification by MinElute PCR Purification Kit from aliquot B; Lane 4 (Input), the library from 1 ng of purified input DNA from the aliquot A. .. Left panel is the profile of H3K4me3 libraries, and right panel is the profile of H3K27me3 libraries. (PDF 123 kb) Mapping results of ChIP-seq reads generated through different purification reagents. (PDF 94 kb) Measuring global ChIP enrichment by FRiP (fraction of reads in peaks).

    Article Title: Purification of nanogram-range immunoprecipitated DNA in ChIP-seq application
    Article Snippet: .. DNA was purified using MinElute PCR Purification Kit after treatment of RNase A and proteinase K. DNA was quantified using Qubit dsDNA High Sensitivity assay and adjusted to 1 ng/μL with TE buffer. .. DNAs were prepared to final 1 ng, 5 ng, 10 ng and 50 ng in 100 μL ChIP elution buffer and were purified by 11 different purification reagents as suggested by the manufacturer except for the elution volume, which was fixed at 16 μL.

    Article Title: Purification of nanogram-range immunoprecipitated DNA in ChIP-seq application
    Article Snippet: .. The ChIP DNA Clean & Concentrator™ (Zymo Research; Zy), the Monarch® PCR & DNA Cleanup Kit (New England Biolabs; Ne), the MinElute PCR Purification Kit (Qiagen, Qm), the QIAquick PCR Purification Kit (Qiagen; Qp), the Agencourt AMPure XP kit (Beckman; Ba) and the RNAClean™ XP kit (Beckman; Br), and phenol/chloroform extraction (Invitrogen; PC) performed well with de-crosslinked chromatin. .. These reagents recovered 78.1% to 95.7% with 10–50 ng of purified DNA, 81.7% to 96.8% with 5 ng of DNA, and 68.1% to 82.9% with 1 ng of DNA except phenol/chloroform extraction with over 100%.

    Article Title: A retrospective study of community-acquired Clostridium difficile infection in southwest China
    Article Snippet: .. PCR ribotyping reaction products were concentrated using a Qiagen Min-Elute PCR purification kit (QIAGEN) and run on a QIAxcel capillary electrophoresis platform (QIAGEN). .. Visualization of PCR products was performed with QIAxcel ScreenGel software (v1.3.0; QIAGEN).

    Article Title: Purification of nanogram-range immunoprecipitated DNA in ChIP-seq application
    Article Snippet: .. For the MinElute PCR Purification Kit, DNA was eluted in Maxyclear and Premium tubes. ..

    Article Title: Purification of nanogram-range immunoprecipitated DNA in ChIP-seq application
    Article Snippet: .. Aliquot A was purified by the lab standard protocol using MinElute PCR Purification Kit in a MaxyClear tube. .. The ChIP enrichment was analyzed by quantitative real-time PCR in positive and negative genomic loci (Additional file ).

    Article Title: Components of the interleukin-33/ST2 system are differentially expressed and regulated in human cardiac cells and in cells of the cardiac vasculature
    Article Snippet: .. For PCR of ST2 isoforms, cDNA was additionally eluted with MinElute PCR Purification Kit (QIAGEN GmbH, Hilden, Germany). .. 2.5 RealTime-PCR RealTime-PCR was performed using LightCycler® TaqMan® Master (Roche) according to the manufacturer's instructions.

    Article Title: Protocol for Metagenomic Virus Detection in Clinical Specimens 1
    Article Snippet: .. Second-strand synthesis was performed by using the NEBNext mRNA Second Strand Synthesis Module (New England Biolabs) and purified by using the MinElute PCR Purification Kit (QIAGEN). .. Double-stranded cDNA, DNA, and random PCR products were quantified by using the Qubit HS dsDNA Kit (Invitrogen Life Technologies).

    Article Title: Purification of nanogram-range immunoprecipitated DNA in ChIP-seq application
    Article Snippet: .. After treatment with RNase A and proteinase K, DNA was purified by Qiagen MinElute PCR Purification Kit (Cat. # 28006, Valencia, CA) and quantified using Qubit dsDNA High Sensitivity assay (Invitrogen, Q32851). .. To check the size of input chromatin, purified input DNA was analyzed by Fragment Analyzer (Advanced Analytical Technologies; AATI; Ankeny, IA) using the High Sensitivity NGS Fragment Analysis Kit (Cat. # DNF-486).

    Article Title: Dysregulated immune system networks in war veterans with PTSD is an outcome of altered miRNA expression and DNA methylation
    Article Snippet: .. After DNA fragments were purified using Qiagen PCR purification kit (Qiagen catalogue #28004), an “A” base was added to the 3′ end of the blunt DNA fragment by Klenow fragment. .. Sequencing adapters were ligated to the ends of DNA fragments using DNA ligase.

    Sequencing:

    Article Title: Protocol for Metagenomic Virus Detection in Clinical Specimens 1
    Article Snippet: Second-strand synthesis was performed by using the NEBNext mRNA Second Strand Synthesis Module (New England Biolabs) and purified by using the MinElute PCR Purification Kit (QIAGEN). .. Sequencing libraries were established by Ion Xpress Plus Fragment Library Kit (without chemical fragmentation) with indices (Ion Xpress Barcode Adapters 1–16 Kit).

    Article Title: Dysregulated immune system networks in war veterans with PTSD is an outcome of altered miRNA expression and DNA methylation
    Article Snippet: After DNA fragments were purified using Qiagen PCR purification kit (Qiagen catalogue #28004), an “A” base was added to the 3′ end of the blunt DNA fragment by Klenow fragment. .. Sequencing adapters were ligated to the ends of DNA fragments using DNA ligase.

    IA:

    Article Title: Purification of nanogram-range immunoprecipitated DNA in ChIP-seq application
    Article Snippet: After treatment with RNase A and proteinase K, DNA was purified by Qiagen MinElute PCR Purification Kit (Cat. # 28006, Valencia, CA) and quantified using Qubit dsDNA High Sensitivity assay (Invitrogen, Q32851). .. To check the size of input chromatin, purified input DNA was analyzed by Fragment Analyzer (Advanced Analytical Technologies; AATI; Ankeny, IA) using the High Sensitivity NGS Fragment Analysis Kit (Cat. # DNF-486).

    Chromatin Immunoprecipitation:

    Article Title: Purification of nanogram-range immunoprecipitated DNA in ChIP-seq application
    Article Snippet: .. Similarly, DNAs were purified from de-crosslinked chromatin estimated to include 1 ng, 5 ng, 10 ng, and 50 ng of DNA after treatment of RNase A and proteinase K. The following reagents were used in the experiment: ChIP DNA Clean & Concentrator™ (Cat. # D5205) from Zymo Research (Zy) (Irvine, CA); Wizard® SV Gel and PCR Clean-Up System (Cat. # A9281) from Promega (Pr) (Fitchburg, WI); GeneJET PCR Purification Kit (Cat. # K0701) from Thermo Fisher Scientific (Th) (Waltham, MA); PureLink® PCR Purification Kit (Cat. # K310001) from Invitrogen (In) (Carlsbad, CA); Monarch® PCR & DNA Cleanup Kit (Cat. # T1030S) from New England Biolabs (Ne) (Ipswich, MA); Chromatin IP DNA Purification Kit (Cat. # 58002) from Active Motif (Am) (Carlsbad, CA); QIAquick PCR Purification Kit (Cat. # 28106) from Qiagen (Qp) (Valencia, CA), MinElute PCR Purification Kit (Cat. # 28006) from Qiagen (Qm); Agencourt AMPure XP (Cat. # A63881) from Beckman (Ba) (Indianapolis, IN), RNAClean™ XP (Cat. # A63987) from Beckman (Br), and phenol/chloroform extraction (PC) (Additional file ). ..

    Article Title: Purification of nanogram-range immunoprecipitated DNA in ChIP-seq application
    Article Snippet: .. Lane 1 (EDL St), the library from 1 ng of purified ChIP DNA from aliquot A; Lane 2 (1 ng QmM), the library from stored DNA in MaxyClear tube after purification by MinElute PCR Purification Kit from aliquot B; Lane 3 (1 ng QmP), the library from stored DNA in Premium Tube after purification by MinElute PCR Purification Kit from aliquot B; Lane 4 (Input), the library from 1 ng of purified input DNA from the aliquot A. .. Left panel is the profile of H3K4me3 libraries, and right panel is the profile of H3K27me3 libraries. (PDF 123 kb) Mapping results of ChIP-seq reads generated through different purification reagents. (PDF 94 kb) Measuring global ChIP enrichment by FRiP (fraction of reads in peaks).

    Article Title: Purification of nanogram-range immunoprecipitated DNA in ChIP-seq application
    Article Snippet: .. The ChIP DNA Clean & Concentrator™ (Zymo Research; Zy), the Monarch® PCR & DNA Cleanup Kit (New England Biolabs; Ne), the MinElute PCR Purification Kit (Qiagen, Qm), the QIAquick PCR Purification Kit (Qiagen; Qp), the Agencourt AMPure XP kit (Beckman; Ba) and the RNAClean™ XP kit (Beckman; Br), and phenol/chloroform extraction (Invitrogen; PC) performed well with de-crosslinked chromatin. .. These reagents recovered 78.1% to 95.7% with 10–50 ng of purified DNA, 81.7% to 96.8% with 5 ng of DNA, and 68.1% to 82.9% with 1 ng of DNA except phenol/chloroform extraction with over 100%.

    Article Title: Purification of nanogram-range immunoprecipitated DNA in ChIP-seq application
    Article Snippet: Paragraph title: Chromatin immunoprecipitation ... After treatment with RNase A and proteinase K, DNA was purified by Qiagen MinElute PCR Purification Kit (Cat. # 28006, Valencia, CA) and quantified using Qubit dsDNA High Sensitivity assay (Invitrogen, Q32851).

    Software:

    Article Title: A retrospective study of community-acquired Clostridium difficile infection in southwest China
    Article Snippet: PCR ribotyping reaction products were concentrated using a Qiagen Min-Elute PCR purification kit (QIAGEN) and run on a QIAxcel capillary electrophoresis platform (QIAGEN). .. Visualization of PCR products was performed with QIAxcel ScreenGel software (v1.3.0; QIAGEN).

    Electrophoresis:

    Article Title: A retrospective study of community-acquired Clostridium difficile infection in southwest China
    Article Snippet: .. PCR ribotyping reaction products were concentrated using a Qiagen Min-Elute PCR purification kit (QIAGEN) and run on a QIAxcel capillary electrophoresis platform (QIAGEN). .. Visualization of PCR products was performed with QIAxcel ScreenGel software (v1.3.0; QIAGEN).

    Agarose Gel Electrophoresis:

    Article Title: Dysregulated immune system networks in war veterans with PTSD is an outcome of altered miRNA expression and DNA methylation
    Article Snippet: After DNA fragments were purified using Qiagen PCR purification kit (Qiagen catalogue #28004), an “A” base was added to the 3′ end of the blunt DNA fragment by Klenow fragment. .. The PCR products were then separated by 2% agarose gel electrophoresis and fragments with sizes ranging from 250 bp to 400 bp were excised and purified using the QIAquick Gel Extraction Kit (Qiagen catalogue #28704).

    Next-Generation Sequencing:

    Article Title: Protocol for Metagenomic Virus Detection in Clinical Specimens 1
    Article Snippet: Paragraph title: NGS ... Second-strand synthesis was performed by using the NEBNext mRNA Second Strand Synthesis Module (New England Biolabs) and purified by using the MinElute PCR Purification Kit (QIAGEN).

    Article Title: Purification of nanogram-range immunoprecipitated DNA in ChIP-seq application
    Article Snippet: After treatment with RNase A and proteinase K, DNA was purified by Qiagen MinElute PCR Purification Kit (Cat. # 28006, Valencia, CA) and quantified using Qubit dsDNA High Sensitivity assay (Invitrogen, Q32851). .. To check the size of input chromatin, purified input DNA was analyzed by Fragment Analyzer (Advanced Analytical Technologies; AATI; Ankeny, IA) using the High Sensitivity NGS Fragment Analysis Kit (Cat. # DNF-486).

    DNA Purification:

    Article Title: Purification of nanogram-range immunoprecipitated DNA in ChIP-seq application
    Article Snippet: .. Similarly, DNAs were purified from de-crosslinked chromatin estimated to include 1 ng, 5 ng, 10 ng, and 50 ng of DNA after treatment of RNase A and proteinase K. The following reagents were used in the experiment: ChIP DNA Clean & Concentrator™ (Cat. # D5205) from Zymo Research (Zy) (Irvine, CA); Wizard® SV Gel and PCR Clean-Up System (Cat. # A9281) from Promega (Pr) (Fitchburg, WI); GeneJET PCR Purification Kit (Cat. # K0701) from Thermo Fisher Scientific (Th) (Waltham, MA); PureLink® PCR Purification Kit (Cat. # K310001) from Invitrogen (In) (Carlsbad, CA); Monarch® PCR & DNA Cleanup Kit (Cat. # T1030S) from New England Biolabs (Ne) (Ipswich, MA); Chromatin IP DNA Purification Kit (Cat. # 58002) from Active Motif (Am) (Carlsbad, CA); QIAquick PCR Purification Kit (Cat. # 28106) from Qiagen (Qp) (Valencia, CA), MinElute PCR Purification Kit (Cat. # 28006) from Qiagen (Qm); Agencourt AMPure XP (Cat. # A63881) from Beckman (Ba) (Indianapolis, IN), RNAClean™ XP (Cat. # A63987) from Beckman (Br), and phenol/chloroform extraction (PC) (Additional file ). ..

    Article Title: Purification of nanogram-range immunoprecipitated DNA in ChIP-seq application
    Article Snippet: The Wizard® SV Gel and PCR Clean-Up System (Promega; Pr), the GeneJET PCR Purification Kit (Thermo Fisher Scientific; Th), the PureLink® PCR Purification Kit (Invitrogen; In) and the Chromatin IP DNA Purification Kit (Active Motif; Am) performed poorly with de-crosslinked chromatin. .. The ChIP DNA Clean & Concentrator™ (Zymo Research; Zy), the Monarch® PCR & DNA Cleanup Kit (New England Biolabs; Ne), the MinElute PCR Purification Kit (Qiagen, Qm), the QIAquick PCR Purification Kit (Qiagen; Qp), the Agencourt AMPure XP kit (Beckman; Ba) and the RNAClean™ XP kit (Beckman; Br), and phenol/chloroform extraction (Invitrogen; PC) performed well with de-crosslinked chromatin.

    Gel Extraction:

    Article Title: Dysregulated immune system networks in war veterans with PTSD is an outcome of altered miRNA expression and DNA methylation
    Article Snippet: After DNA fragments were purified using Qiagen PCR purification kit (Qiagen catalogue #28004), an “A” base was added to the 3′ end of the blunt DNA fragment by Klenow fragment. .. The PCR products were then separated by 2% agarose gel electrophoresis and fragments with sizes ranging from 250 bp to 400 bp were excised and purified using the QIAquick Gel Extraction Kit (Qiagen catalogue #28704).

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    Qiagen minelute pcr purification kit
    DNA purification reagents vary in their ability to recover low amounts of DNA from de-crosslinked chromatin. a Recovered DNA amount by different DNA purification reagents from de-crosslinked chromatin. De-crosslinked chromatin estimated to include 1 ng range DNA in ChIP elution buffer was purified following the manufacturer’s instructions. The data were generated from triplicate DNA samples derived from three independent preparations. Zy, ChIP DNA Clean Concentrator™ (Zymo Research); Pr, Wizard® SV Gel and <t>PCR</t> Clean-Up System (Promega); Th, GeneJET PCR Purification Kit (Thermo Fisher Scientific); In, PureLink® PCR Purification Kit (Invitrogen); Ne, Monarch® PCR DNA Cleanup Kit (New England Biolabs); Am, Chromatin IP DNA Purification Kit (Active Motif); Qp, QIAquick PCR Purification Kit (Qiagen); Qm, <t>MinElute</t> PCR Purification Kit (Qiagen); Ba, Agencourt AMPure XP kit (Beckman, chromatin to beads ratio from 1:1.25 to 1:2); Br, RNAClean™ XP kit (Beckman, chromatin to beads ratio from 1:1.25 to 1:2); PC, phenol/chloroform extraction. b Interference of PCR amplification by purified eluent of purification reagents. 9 μL eluent was mixed with 1 μL 166 bp of Drosophila probe DNA (0.0001 ng), and the resulting mixture was used as the template in 20 μl of real-time PCR reaction. The Ct value for Drosophila probe DNA from TE buffer was set as 100%. The experiment was repeated 3 times using de-crosslinked chromatin estimated to include 1 ng of DNA. c Size profiles of DNA purified by different reagents. The DNAs purified from de-crosslinked chromatin estimated to include 50 ng range DNA was analyzed by AATI Fragment Analyzer. DNA size (bp) is shown
    Minelute Pcr Purification Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 2289 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    DNA purification reagents vary in their ability to recover low amounts of DNA from de-crosslinked chromatin. a Recovered DNA amount by different DNA purification reagents from de-crosslinked chromatin. De-crosslinked chromatin estimated to include 1 ng range DNA in ChIP elution buffer was purified following the manufacturer’s instructions. The data were generated from triplicate DNA samples derived from three independent preparations. Zy, ChIP DNA Clean Concentrator™ (Zymo Research); Pr, Wizard® SV Gel and PCR Clean-Up System (Promega); Th, GeneJET PCR Purification Kit (Thermo Fisher Scientific); In, PureLink® PCR Purification Kit (Invitrogen); Ne, Monarch® PCR DNA Cleanup Kit (New England Biolabs); Am, Chromatin IP DNA Purification Kit (Active Motif); Qp, QIAquick PCR Purification Kit (Qiagen); Qm, MinElute PCR Purification Kit (Qiagen); Ba, Agencourt AMPure XP kit (Beckman, chromatin to beads ratio from 1:1.25 to 1:2); Br, RNAClean™ XP kit (Beckman, chromatin to beads ratio from 1:1.25 to 1:2); PC, phenol/chloroform extraction. b Interference of PCR amplification by purified eluent of purification reagents. 9 μL eluent was mixed with 1 μL 166 bp of Drosophila probe DNA (0.0001 ng), and the resulting mixture was used as the template in 20 μl of real-time PCR reaction. The Ct value for Drosophila probe DNA from TE buffer was set as 100%. The experiment was repeated 3 times using de-crosslinked chromatin estimated to include 1 ng of DNA. c Size profiles of DNA purified by different reagents. The DNAs purified from de-crosslinked chromatin estimated to include 50 ng range DNA was analyzed by AATI Fragment Analyzer. DNA size (bp) is shown

    Journal: BMC Genomics

    Article Title: Purification of nanogram-range immunoprecipitated DNA in ChIP-seq application

    doi: 10.1186/s12864-017-4371-5

    Figure Lengend Snippet: DNA purification reagents vary in their ability to recover low amounts of DNA from de-crosslinked chromatin. a Recovered DNA amount by different DNA purification reagents from de-crosslinked chromatin. De-crosslinked chromatin estimated to include 1 ng range DNA in ChIP elution buffer was purified following the manufacturer’s instructions. The data were generated from triplicate DNA samples derived from three independent preparations. Zy, ChIP DNA Clean Concentrator™ (Zymo Research); Pr, Wizard® SV Gel and PCR Clean-Up System (Promega); Th, GeneJET PCR Purification Kit (Thermo Fisher Scientific); In, PureLink® PCR Purification Kit (Invitrogen); Ne, Monarch® PCR DNA Cleanup Kit (New England Biolabs); Am, Chromatin IP DNA Purification Kit (Active Motif); Qp, QIAquick PCR Purification Kit (Qiagen); Qm, MinElute PCR Purification Kit (Qiagen); Ba, Agencourt AMPure XP kit (Beckman, chromatin to beads ratio from 1:1.25 to 1:2); Br, RNAClean™ XP kit (Beckman, chromatin to beads ratio from 1:1.25 to 1:2); PC, phenol/chloroform extraction. b Interference of PCR amplification by purified eluent of purification reagents. 9 μL eluent was mixed with 1 μL 166 bp of Drosophila probe DNA (0.0001 ng), and the resulting mixture was used as the template in 20 μl of real-time PCR reaction. The Ct value for Drosophila probe DNA from TE buffer was set as 100%. The experiment was repeated 3 times using de-crosslinked chromatin estimated to include 1 ng of DNA. c Size profiles of DNA purified by different reagents. The DNAs purified from de-crosslinked chromatin estimated to include 50 ng range DNA was analyzed by AATI Fragment Analyzer. DNA size (bp) is shown

    Article Snippet: The ChIP DNA Clean & Concentrator™ (Zymo Research; Zy), the Monarch® PCR & DNA Cleanup Kit (New England Biolabs; Ne), the MinElute PCR Purification Kit (Qiagen, Qm), the QIAquick PCR Purification Kit (Qiagen; Qp), the Agencourt AMPure XP kit (Beckman; Ba) and the RNAClean™ XP kit (Beckman; Br), and phenol/chloroform extraction (Invitrogen; PC) performed well with de-crosslinked chromatin.

    Techniques: DNA Purification, Chromatin Immunoprecipitation, Purification, Generated, Derivative Assay, Polymerase Chain Reaction, Amplification, Real-time Polymerase Chain Reaction