minelute gel extration kit  (Qiagen)

 
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    Name:
    MinElute Gel Extraction Kit
    Description:
    For gel extraction of up to 5 μg DNA fragments 70 bp to 4 kb in low elution volumes Kit contents Qiagen MinElute Gel Extraction Kit 50 MinElute Spin Columns 5g Binding Capacity 10L Elution Volume Tube Format Gel Extraction Technology 70 bp to 4 kb Fragment Size Manual Processing DNA Sample Fast Procedure and Easy Handling High Reproducible Recoveries For Gel Extraction of up to 5μg DNA fragments in Low Elution Volumes Includes 50 MinElute Spin Columns Buffers Collection Tubes 2mL Benefits Very small elution volumes Fast procedure and easy handling High reproducible recoveries Gel loading dye for convenient sample analysis
    Catalog Number:
    28604
    Price:
    136
    Category:
    MinElute Gel Extraction Kit
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    Structured Review

    Qiagen minelute gel extration kit
    MinElute Gel Extraction Kit
    For gel extraction of up to 5 μg DNA fragments 70 bp to 4 kb in low elution volumes Kit contents Qiagen MinElute Gel Extraction Kit 50 MinElute Spin Columns 5g Binding Capacity 10L Elution Volume Tube Format Gel Extraction Technology 70 bp to 4 kb Fragment Size Manual Processing DNA Sample Fast Procedure and Easy Handling High Reproducible Recoveries For Gel Extraction of up to 5μg DNA fragments in Low Elution Volumes Includes 50 MinElute Spin Columns Buffers Collection Tubes 2mL Benefits Very small elution volumes Fast procedure and easy handling High reproducible recoveries Gel loading dye for convenient sample analysis
    https://www.bioz.com/result/minelute gel extration kit/product/Qiagen
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    minelute gel extration kit - by Bioz Stars, 2020-09
    99/100 stars

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    Related Articles

    Nucleic Acid Electrophoresis:

    Article Title: The DN2 Myeloid-T (DN2mt) Progenitor is a Target Cell for Leukemic Transformation by the TLX1 Oncogene
    Article Snippet: .. Nested PCR products were separated by gel electrophoresis on 1% agarose gels in TAE buffer and bands excised and purified using the Qiagen MinElute Gel Extraction Kit (Qiagen). .. All PCR amplifications were performed using Platinum Taq (Invitrogen).

    Amplification:

    Article Title: How a Retrotransposon Exploits the Plant's Heat Stress Response for Its Activation
    Article Snippet: .. After a PCR amplification step, using ONSEN -SNPs F and R primers (Supplementary ), the PCR product was purified with the MinElute Gel Extraction Kit (Qiagen) and ligated using the InsTAclone PCR Cloning Kit (Thermo Scientific). .. Individual clones were Sanger-sequenced.

    Article Title: Transglycosylation by Chitinase D from Serratia proteamaculans
    Article Snippet: .. Agarose gel electrophoresis was performed, and the amplicon of desired size was gel-eluted using the gel extraction kit from Qiagen (Duesseldorf, Germany). .. Both the gel-eluted amplicons and expression vector pET-22b(+) were subjected to double digestion with NcoI and XhoI at 37 °C for 6 h, followed by PCR clean up using the Qiagen gel clean up kit.

    Agarose Gel Electrophoresis:

    Article Title: Expression, purification of metallothionein genes from freshwater crab (Sinopotamon yangtsekiense) and development of an anti-metallothionein ELISA
    Article Snippet: .. Reaction was carried out with an initial denaturation step at 95°C for 5 min, followed by 30 cycles of 94°C for 30 s, 65°C for 30 s, 70°C for 1 min, and a final step of 70°C for 10 min. PCR fragments were detected by agarose gel electrophoresis, and extracted using DNA Gel Extraction Kit (Qiagen, Hilden, Germany). .. By T4 DNA ligase (New England Biolabs, Ipswich, MA), the extracted fragment digested with NcoI and BamHI was ligased to vector phoA at 4°C, and was transformed into DH5α competent cells.

    Article Title: Transglycosylation by Chitinase D from Serratia proteamaculans
    Article Snippet: .. Agarose gel electrophoresis was performed, and the amplicon of desired size was gel-eluted using the gel extraction kit from Qiagen (Duesseldorf, Germany). .. Both the gel-eluted amplicons and expression vector pET-22b(+) were subjected to double digestion with NcoI and XhoI at 37 °C for 6 h, followed by PCR clean up using the Qiagen gel clean up kit.

    Clone Assay:

    Article Title: How a Retrotransposon Exploits the Plant's Heat Stress Response for Its Activation
    Article Snippet: .. After a PCR amplification step, using ONSEN -SNPs F and R primers (Supplementary ), the PCR product was purified with the MinElute Gel Extraction Kit (Qiagen) and ligated using the InsTAclone PCR Cloning Kit (Thermo Scientific). .. Individual clones were Sanger-sequenced.

    TA Cloning:

    Article Title: mRNA Distribution and Heterologous Expression of Orphan Cytochrome P450 20A1
    Article Snippet: .. The PCR products were purified using a QIAGEN Gel Extraction Kit™ (Qiagen, Valencia, CA) and ligated into a PCR2.1 TOPO vector (TOPO TA Cloning Kit™ for sequencing, Invitrogen). .. Chemically-competent One-shot™ E. coli cells (Invitrogen) were transformed, and single clones were used to prepare plasmid DNA.

    Purification:

    Article Title: mRNA Distribution and Heterologous Expression of Orphan Cytochrome P450 20A1
    Article Snippet: .. The PCR products were purified using a QIAGEN Gel Extraction Kit™ (Qiagen, Valencia, CA) and ligated into a PCR2.1 TOPO vector (TOPO TA Cloning Kit™ for sequencing, Invitrogen). .. Chemically-competent One-shot™ E. coli cells (Invitrogen) were transformed, and single clones were used to prepare plasmid DNA.

    Article Title: The DN2 Myeloid-T (DN2mt) Progenitor is a Target Cell for Leukemic Transformation by the TLX1 Oncogene
    Article Snippet: .. Nested PCR products were separated by gel electrophoresis on 1% agarose gels in TAE buffer and bands excised and purified using the Qiagen MinElute Gel Extraction Kit (Qiagen). .. All PCR amplifications were performed using Platinum Taq (Invitrogen).

    Article Title: How a Retrotransposon Exploits the Plant's Heat Stress Response for Its Activation
    Article Snippet: .. After a PCR amplification step, using ONSEN -SNPs F and R primers (Supplementary ), the PCR product was purified with the MinElute Gel Extraction Kit (Qiagen) and ligated using the InsTAclone PCR Cloning Kit (Thermo Scientific). .. Individual clones were Sanger-sequenced.

    Polymerase Chain Reaction:

    Article Title: mRNA Distribution and Heterologous Expression of Orphan Cytochrome P450 20A1
    Article Snippet: .. The PCR products were purified using a QIAGEN Gel Extraction Kit™ (Qiagen, Valencia, CA) and ligated into a PCR2.1 TOPO vector (TOPO TA Cloning Kit™ for sequencing, Invitrogen). .. Chemically-competent One-shot™ E. coli cells (Invitrogen) were transformed, and single clones were used to prepare plasmid DNA.

    Article Title: How a Retrotransposon Exploits the Plant's Heat Stress Response for Its Activation
    Article Snippet: .. After a PCR amplification step, using ONSEN -SNPs F and R primers (Supplementary ), the PCR product was purified with the MinElute Gel Extraction Kit (Qiagen) and ligated using the InsTAclone PCR Cloning Kit (Thermo Scientific). .. Individual clones were Sanger-sequenced.

    Article Title: Expression, purification of metallothionein genes from freshwater crab (Sinopotamon yangtsekiense) and development of an anti-metallothionein ELISA
    Article Snippet: .. Reaction was carried out with an initial denaturation step at 95°C for 5 min, followed by 30 cycles of 94°C for 30 s, 65°C for 30 s, 70°C for 1 min, and a final step of 70°C for 10 min. PCR fragments were detected by agarose gel electrophoresis, and extracted using DNA Gel Extraction Kit (Qiagen, Hilden, Germany). .. By T4 DNA ligase (New England Biolabs, Ipswich, MA), the extracted fragment digested with NcoI and BamHI was ligased to vector phoA at 4°C, and was transformed into DH5α competent cells.

    Sequencing:

    Article Title: mRNA Distribution and Heterologous Expression of Orphan Cytochrome P450 20A1
    Article Snippet: .. The PCR products were purified using a QIAGEN Gel Extraction Kit™ (Qiagen, Valencia, CA) and ligated into a PCR2.1 TOPO vector (TOPO TA Cloning Kit™ for sequencing, Invitrogen). .. Chemically-competent One-shot™ E. coli cells (Invitrogen) were transformed, and single clones were used to prepare plasmid DNA.

    Gel Extraction:

    Article Title: mRNA Distribution and Heterologous Expression of Orphan Cytochrome P450 20A1
    Article Snippet: .. The PCR products were purified using a QIAGEN Gel Extraction Kit™ (Qiagen, Valencia, CA) and ligated into a PCR2.1 TOPO vector (TOPO TA Cloning Kit™ for sequencing, Invitrogen). .. Chemically-competent One-shot™ E. coli cells (Invitrogen) were transformed, and single clones were used to prepare plasmid DNA.

    Article Title: Dual fluorescence detection of protein and RNA in Drosophila tissues
    Article Snippet: .. 2 Gel-purify the linearized DNA template with the Qiagen gel extraction kit according to the manufacturer’s protocol. ..

    Article Title: The DN2 Myeloid-T (DN2mt) Progenitor is a Target Cell for Leukemic Transformation by the TLX1 Oncogene
    Article Snippet: .. Nested PCR products were separated by gel electrophoresis on 1% agarose gels in TAE buffer and bands excised and purified using the Qiagen MinElute Gel Extraction Kit (Qiagen). .. All PCR amplifications were performed using Platinum Taq (Invitrogen).

    Article Title: How a Retrotransposon Exploits the Plant's Heat Stress Response for Its Activation
    Article Snippet: .. After a PCR amplification step, using ONSEN -SNPs F and R primers (Supplementary ), the PCR product was purified with the MinElute Gel Extraction Kit (Qiagen) and ligated using the InsTAclone PCR Cloning Kit (Thermo Scientific). .. Individual clones were Sanger-sequenced.

    Article Title: Expression, purification of metallothionein genes from freshwater crab (Sinopotamon yangtsekiense) and development of an anti-metallothionein ELISA
    Article Snippet: .. Reaction was carried out with an initial denaturation step at 95°C for 5 min, followed by 30 cycles of 94°C for 30 s, 65°C for 30 s, 70°C for 1 min, and a final step of 70°C for 10 min. PCR fragments were detected by agarose gel electrophoresis, and extracted using DNA Gel Extraction Kit (Qiagen, Hilden, Germany). .. By T4 DNA ligase (New England Biolabs, Ipswich, MA), the extracted fragment digested with NcoI and BamHI was ligased to vector phoA at 4°C, and was transformed into DH5α competent cells.

    Article Title: Global Analysis of Transcription Factor-Binding Sites in Yeast Using ChIP-Seq
    Article Snippet: .. MinElute and QIAquick gel extraction kits (Qiagen). .. Illumina Genome Analyzer sequencer, with reagents, flowcell, and cluster station.

    Article Title: Transglycosylation by Chitinase D from Serratia proteamaculans
    Article Snippet: .. Agarose gel electrophoresis was performed, and the amplicon of desired size was gel-eluted using the gel extraction kit from Qiagen (Duesseldorf, Germany). .. Both the gel-eluted amplicons and expression vector pET-22b(+) were subjected to double digestion with NcoI and XhoI at 37 °C for 6 h, followed by PCR clean up using the Qiagen gel clean up kit.

    Nested PCR:

    Article Title: The DN2 Myeloid-T (DN2mt) Progenitor is a Target Cell for Leukemic Transformation by the TLX1 Oncogene
    Article Snippet: .. Nested PCR products were separated by gel electrophoresis on 1% agarose gels in TAE buffer and bands excised and purified using the Qiagen MinElute Gel Extraction Kit (Qiagen). .. All PCR amplifications were performed using Platinum Taq (Invitrogen).

    Plasmid Preparation:

    Article Title: mRNA Distribution and Heterologous Expression of Orphan Cytochrome P450 20A1
    Article Snippet: .. The PCR products were purified using a QIAGEN Gel Extraction Kit™ (Qiagen, Valencia, CA) and ligated into a PCR2.1 TOPO vector (TOPO TA Cloning Kit™ for sequencing, Invitrogen). .. Chemically-competent One-shot™ E. coli cells (Invitrogen) were transformed, and single clones were used to prepare plasmid DNA.

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  • 99
    Qiagen minelute gel extraction kit
    Minelute Gel Extraction Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 12804 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/minelute gel extraction kit/product/Qiagen
    Average 99 stars, based on 12804 article reviews
    Price from $9.99 to $1999.99
    minelute gel extraction kit - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

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