minelute gel extraction kit  (Qiagen)

 
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    Name:
    MinElute Gel Extraction Kit
    Description:
    For gel extraction of up to 5 μg DNA fragments 70 bp to 4 kb in low elution volumes Kit contents Qiagen MinElute Gel Extraction Kit 50 MinElute Spin Columns 5g Binding Capacity 10L Elution Volume Tube Format Gel Extraction Technology 70 bp to 4 kb Fragment Size Manual Processing DNA Sample Fast Procedure and Easy Handling High Reproducible Recoveries For Gel Extraction of up to 5μg DNA fragments in Low Elution Volumes Includes 50 MinElute Spin Columns Buffers Collection Tubes 2mL Benefits Very small elution volumes Fast procedure and easy handling High reproducible recoveries Gel loading dye for convenient sample analysis
    Catalog Number:
    28604
    Price:
    136
    Category:
    MinElute Gel Extraction Kit
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    Qiagen minelute gel extraction kit
    MinElute Gel Extraction Kit
    For gel extraction of up to 5 μg DNA fragments 70 bp to 4 kb in low elution volumes Kit contents Qiagen MinElute Gel Extraction Kit 50 MinElute Spin Columns 5g Binding Capacity 10L Elution Volume Tube Format Gel Extraction Technology 70 bp to 4 kb Fragment Size Manual Processing DNA Sample Fast Procedure and Easy Handling High Reproducible Recoveries For Gel Extraction of up to 5μg DNA fragments in Low Elution Volumes Includes 50 MinElute Spin Columns Buffers Collection Tubes 2mL Benefits Very small elution volumes Fast procedure and easy handling High reproducible recoveries Gel loading dye for convenient sample analysis
    https://www.bioz.com/result/minelute gel extraction kit/product/Qiagen
    Average 99 stars, based on 1114 article reviews
    Price from $9.99 to $1999.99
    minelute gel extraction kit - by Bioz Stars, 2020-07
    99/100 stars

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    Related Articles

    Nucleic Acid Electrophoresis:

    Article Title: Field resistance to Fusarium oxysporum and Verticillium dahliae in transgenic cotton expressing the plant defensin NaD1
    Article Snippet: .. Amplified fragments were separated by gel electrophoresis, gel-purified (MinElute gel extraction kit, Qiagen), and directly sequenced using the second-round oligonucleotides (Macrogen, South Korea). .. Detection of NaD1 by immunoblot analysis Total soluble proteins were extracted from plant tissue in 50mM H2 SO4 plus 2% (w/v) polyvinylpyrrolidone (PVPP; 1g fresh weight tissue (1.2 ml buffer)–1 ) and insoluble material was removed by centrifugation (18,400 g , 10min).

    Amplification:

    Article Title: Field resistance to Fusarium oxysporum and Verticillium dahliae in transgenic cotton expressing the plant defensin NaD1
    Article Snippet: .. Amplified fragments were separated by gel electrophoresis, gel-purified (MinElute gel extraction kit, Qiagen), and directly sequenced using the second-round oligonucleotides (Macrogen, South Korea). .. Detection of NaD1 by immunoblot analysis Total soluble proteins were extracted from plant tissue in 50mM H2 SO4 plus 2% (w/v) polyvinylpyrrolidone (PVPP; 1g fresh weight tissue (1.2 ml buffer)–1 ) and insoluble material was removed by centrifugation (18,400 g , 10min).

    Agarose Gel Electrophoresis:

    Article Title: Expression, purification of metallothionein genes from freshwater crab (Sinopotamon yangtsekiense) and development of an anti-metallothionein ELISA
    Article Snippet: .. Reaction was carried out with an initial denaturation step at 95°C for 5 min, followed by 30 cycles of 94°C for 30 s, 65°C for 30 s, 70°C for 1 min, and a final step of 70°C for 10 min. PCR fragments were detected by agarose gel electrophoresis, and extracted using DNA Gel Extraction Kit (Qiagen, Hilden, Germany). .. By T4 DNA ligase (New England Biolabs, Ipswich, MA), the extracted fragment digested with NcoI and BamHI was ligased to vector phoA at 4°C, and was transformed into DH5α competent cells.

    Purification:

    Article Title: Multiplex CRISPR/Cas9-Based Genome Editing for Correction of Dystrophin Mutations that Cause Duchenne Muscular Dystrophy
    Article Snippet: .. PCR amplicons corresponding to the expected length of predicted translocations and only present in cells treated with sgRNA were purified (QIAGEN Gel Extraction kit) and analyzed by Sanger sequencing. .. mRNA analysis Immortalized myoblasts were differentiated into myofibers by replacing the growth medium with DMEM supplemented with 1% insulin-transferrin-selenium (Invitrogen #51500056) and 1% penicillin/streptomycin (Invitrogen #15140) for 5 days before the cells were trypsinized and collected.

    Article Title: Genetic transformation of the dinoflagellate chloroplast
    Article Snippet: .. PCR products were purified from excised gel pieces using the MinElute gel extraction kit (Qiagen). .. Some PCR products were directly sequenced after gel extraction whilst others were ligated into the pGEM-T Easy plasmid vector (Promega), following the manufacturer’s instructions.

    Article Title: Catalysts from synthetic genetic polymers
    Article Snippet: .. Bands of appropriate size were purified using a gel extraction kit (Qiagen, Netherlands) as per manufacturer’s instructions. .. Purified DNA was used as the polyclonal template for either sequencing library PCR (see below) or large scale preparative PCR (2ml) for generation of DNA templates for XNA synthesis.

    Article Title: Biogeography of Soil Bacterial Networks along a Gradient of Cropping Intensity
    Article Snippet: .. Crude DNA was then purified using a MinElute gel extraction kit (Qiagen, France) and quantified using a QuantiFluor staining kit (Promega, USA), prior to further investigations. .. PCR amplification and pyrosequencing of 16S rRNA gene sequences A 16S rRNA gene fragment targeting the V3-V4 regions to characterize bacterial diversity was amplified using the primers F479 (5′-CAGCMGCYGCNGTAANAC-3′) and R888 (5′-CCGYCAATTCMTTTRAGT-3′) with the method described previously .

    Polymerase Chain Reaction:

    Article Title: Multiplex CRISPR/Cas9-Based Genome Editing for Correction of Dystrophin Mutations that Cause Duchenne Muscular Dystrophy
    Article Snippet: .. PCR amplicons corresponding to the expected length of predicted translocations and only present in cells treated with sgRNA were purified (QIAGEN Gel Extraction kit) and analyzed by Sanger sequencing. .. mRNA analysis Immortalized myoblasts were differentiated into myofibers by replacing the growth medium with DMEM supplemented with 1% insulin-transferrin-selenium (Invitrogen #51500056) and 1% penicillin/streptomycin (Invitrogen #15140) for 5 days before the cells were trypsinized and collected.

    Article Title: Expression, purification of metallothionein genes from freshwater crab (Sinopotamon yangtsekiense) and development of an anti-metallothionein ELISA
    Article Snippet: .. Reaction was carried out with an initial denaturation step at 95°C for 5 min, followed by 30 cycles of 94°C for 30 s, 65°C for 30 s, 70°C for 1 min, and a final step of 70°C for 10 min. PCR fragments were detected by agarose gel electrophoresis, and extracted using DNA Gel Extraction Kit (Qiagen, Hilden, Germany). .. By T4 DNA ligase (New England Biolabs, Ipswich, MA), the extracted fragment digested with NcoI and BamHI was ligased to vector phoA at 4°C, and was transformed into DH5α competent cells.

    Article Title: Genetic transformation of the dinoflagellate chloroplast
    Article Snippet: .. PCR products were purified from excised gel pieces using the MinElute gel extraction kit (Qiagen). .. Some PCR products were directly sequenced after gel extraction whilst others were ligated into the pGEM-T Easy plasmid vector (Promega), following the manufacturer’s instructions.

    Article Title: HLA genotyping by next-generation sequencing of complementary DNA
    Article Snippet: .. The following cycling conditions were used: 1 cycle of 94 °C for 30 s, 40 cycles of 94 °C for 30 s, 65 °C for 30 s, and 68 °C for 1 min. After mixing each PCR product, gel extraction (range from 200 bp to 500 bp) was performed using the MinElute Gel Extraction Kit (Qiagen, Hilden, Germany). .. The concentration of the purified products was measured using the Quanti-iT™ dsDNA Assay Kit, Broad range.

    Gel Extraction:

    Article Title: Multiplex CRISPR/Cas9-Based Genome Editing for Correction of Dystrophin Mutations that Cause Duchenne Muscular Dystrophy
    Article Snippet: .. PCR amplicons corresponding to the expected length of predicted translocations and only present in cells treated with sgRNA were purified (QIAGEN Gel Extraction kit) and analyzed by Sanger sequencing. .. mRNA analysis Immortalized myoblasts were differentiated into myofibers by replacing the growth medium with DMEM supplemented with 1% insulin-transferrin-selenium (Invitrogen #51500056) and 1% penicillin/streptomycin (Invitrogen #15140) for 5 days before the cells were trypsinized and collected.

    Article Title: Field resistance to Fusarium oxysporum and Verticillium dahliae in transgenic cotton expressing the plant defensin NaD1
    Article Snippet: .. Amplified fragments were separated by gel electrophoresis, gel-purified (MinElute gel extraction kit, Qiagen), and directly sequenced using the second-round oligonucleotides (Macrogen, South Korea). .. Detection of NaD1 by immunoblot analysis Total soluble proteins were extracted from plant tissue in 50mM H2 SO4 plus 2% (w/v) polyvinylpyrrolidone (PVPP; 1g fresh weight tissue (1.2 ml buffer)–1 ) and insoluble material was removed by centrifugation (18,400 g , 10min).

    Article Title: Expression, purification of metallothionein genes from freshwater crab (Sinopotamon yangtsekiense) and development of an anti-metallothionein ELISA
    Article Snippet: .. Reaction was carried out with an initial denaturation step at 95°C for 5 min, followed by 30 cycles of 94°C for 30 s, 65°C for 30 s, 70°C for 1 min, and a final step of 70°C for 10 min. PCR fragments were detected by agarose gel electrophoresis, and extracted using DNA Gel Extraction Kit (Qiagen, Hilden, Germany). .. By T4 DNA ligase (New England Biolabs, Ipswich, MA), the extracted fragment digested with NcoI and BamHI was ligased to vector phoA at 4°C, and was transformed into DH5α competent cells.

    Article Title: Genetic transformation of the dinoflagellate chloroplast
    Article Snippet: .. PCR products were purified from excised gel pieces using the MinElute gel extraction kit (Qiagen). .. Some PCR products were directly sequenced after gel extraction whilst others were ligated into the pGEM-T Easy plasmid vector (Promega), following the manufacturer’s instructions.

    Article Title: Global Analysis of Transcription Factor-Binding Sites in Yeast Using ChIP-Seq
    Article Snippet: .. MinElute and QIAquick gel extraction kits (Qiagen). .. Illumina Genome Analyzer sequencer, with reagents, flowcell, and cluster station.

    Article Title: Catalysts from synthetic genetic polymers
    Article Snippet: .. Bands of appropriate size were purified using a gel extraction kit (Qiagen, Netherlands) as per manufacturer’s instructions. .. Purified DNA was used as the polyclonal template for either sequencing library PCR (see below) or large scale preparative PCR (2ml) for generation of DNA templates for XNA synthesis.

    Article Title: Biogeography of Soil Bacterial Networks along a Gradient of Cropping Intensity
    Article Snippet: .. Crude DNA was then purified using a MinElute gel extraction kit (Qiagen, France) and quantified using a QuantiFluor staining kit (Promega, USA), prior to further investigations. .. PCR amplification and pyrosequencing of 16S rRNA gene sequences A 16S rRNA gene fragment targeting the V3-V4 regions to characterize bacterial diversity was amplified using the primers F479 (5′-CAGCMGCYGCNGTAANAC-3′) and R888 (5′-CCGYCAATTCMTTTRAGT-3′) with the method described previously .

    Article Title: HLA genotyping by next-generation sequencing of complementary DNA
    Article Snippet: .. The following cycling conditions were used: 1 cycle of 94 °C for 30 s, 40 cycles of 94 °C for 30 s, 65 °C for 30 s, and 68 °C for 1 min. After mixing each PCR product, gel extraction (range from 200 bp to 500 bp) was performed using the MinElute Gel Extraction Kit (Qiagen, Hilden, Germany). .. The concentration of the purified products was measured using the Quanti-iT™ dsDNA Assay Kit, Broad range.

    Sequencing:

    Article Title: Multiplex CRISPR/Cas9-Based Genome Editing for Correction of Dystrophin Mutations that Cause Duchenne Muscular Dystrophy
    Article Snippet: .. PCR amplicons corresponding to the expected length of predicted translocations and only present in cells treated with sgRNA were purified (QIAGEN Gel Extraction kit) and analyzed by Sanger sequencing. .. mRNA analysis Immortalized myoblasts were differentiated into myofibers by replacing the growth medium with DMEM supplemented with 1% insulin-transferrin-selenium (Invitrogen #51500056) and 1% penicillin/streptomycin (Invitrogen #15140) for 5 days before the cells were trypsinized and collected.

    Staining:

    Article Title: Biogeography of Soil Bacterial Networks along a Gradient of Cropping Intensity
    Article Snippet: .. Crude DNA was then purified using a MinElute gel extraction kit (Qiagen, France) and quantified using a QuantiFluor staining kit (Promega, USA), prior to further investigations. .. PCR amplification and pyrosequencing of 16S rRNA gene sequences A 16S rRNA gene fragment targeting the V3-V4 regions to characterize bacterial diversity was amplified using the primers F479 (5′-CAGCMGCYGCNGTAANAC-3′) and R888 (5′-CCGYCAATTCMTTTRAGT-3′) with the method described previously .

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  • 99
    Qiagen minelute gel extraction kit
    Minelute Gel Extraction Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 1114 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/minelute gel extraction kit/product/Qiagen
    Average 99 stars, based on 1114 article reviews
    Price from $9.99 to $1999.99
    minelute gel extraction kit - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

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