minelute columns  (Qiagen)

 
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    MinElute Gel Extraction Kit
    Description:
    For gel extraction of up to 5 μg DNA fragments 70 bp to 4 kb in low elution volumes Kit contents Qiagen MinElute Gel Extraction Kit 50 MinElute Spin Columns 5g Binding Capacity 10L Elution Volume Tube Format Gel Extraction Technology 70 bp to 4 kb Fragment Size Manual Processing DNA Sample Fast Procedure and Easy Handling High Reproducible Recoveries For Gel Extraction of up to 5μg DNA fragments in Low Elution Volumes Includes 50 MinElute Spin Columns Buffers Collection Tubes 2mL Benefits Very small elution volumes Fast procedure and easy handling High reproducible recoveries Gel loading dye for convenient sample analysis
    Catalog Number:
    28604
    Price:
    136
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    MinElute Gel Extraction Kit
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    Qiagen minelute columns
    MinElute Gel Extraction Kit
    For gel extraction of up to 5 μg DNA fragments 70 bp to 4 kb in low elution volumes Kit contents Qiagen MinElute Gel Extraction Kit 50 MinElute Spin Columns 5g Binding Capacity 10L Elution Volume Tube Format Gel Extraction Technology 70 bp to 4 kb Fragment Size Manual Processing DNA Sample Fast Procedure and Easy Handling High Reproducible Recoveries For Gel Extraction of up to 5μg DNA fragments in Low Elution Volumes Includes 50 MinElute Spin Columns Buffers Collection Tubes 2mL Benefits Very small elution volumes Fast procedure and easy handling High reproducible recoveries Gel loading dye for convenient sample analysis
    https://www.bioz.com/result/minelute columns/product/Qiagen
    Average 99 stars, based on 247 article reviews
    Price from $9.99 to $1999.99
    minelute columns - by Bioz Stars, 2020-07
    99/100 stars

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    Related Articles

    Nucleic Acid Electrophoresis:

    Article Title: Field resistance to Fusarium oxysporum and Verticillium dahliae in transgenic cotton expressing the plant defensin NaD1
    Article Snippet: .. Amplified fragments were separated by gel electrophoresis, gel-purified (MinElute gel extraction kit, Qiagen), and directly sequenced using the second-round oligonucleotides (Macrogen, South Korea). .. Detection of NaD1 by immunoblot analysis Total soluble proteins were extracted from plant tissue in 50mM H2 SO4 plus 2% (w/v) polyvinylpyrrolidone (PVPP; 1g fresh weight tissue (1.2 ml buffer)–1 ) and insoluble material was removed by centrifugation (18,400 g , 10min).

    Amplification:

    Article Title: Field resistance to Fusarium oxysporum and Verticillium dahliae in transgenic cotton expressing the plant defensin NaD1
    Article Snippet: .. Amplified fragments were separated by gel electrophoresis, gel-purified (MinElute gel extraction kit, Qiagen), and directly sequenced using the second-round oligonucleotides (Macrogen, South Korea). .. Detection of NaD1 by immunoblot analysis Total soluble proteins were extracted from plant tissue in 50mM H2 SO4 plus 2% (w/v) polyvinylpyrrolidone (PVPP; 1g fresh weight tissue (1.2 ml buffer)–1 ) and insoluble material was removed by centrifugation (18,400 g , 10min).

    Agarose Gel Electrophoresis:

    Article Title: Expression, purification of metallothionein genes from freshwater crab (Sinopotamon yangtsekiense) and development of an anti-metallothionein ELISA
    Article Snippet: .. Reaction was carried out with an initial denaturation step at 95°C for 5 min, followed by 30 cycles of 94°C for 30 s, 65°C for 30 s, 70°C for 1 min, and a final step of 70°C for 10 min. PCR fragments were detected by agarose gel electrophoresis, and extracted using DNA Gel Extraction Kit (Qiagen, Hilden, Germany). .. By T4 DNA ligase (New England Biolabs, Ipswich, MA), the extracted fragment digested with NcoI and BamHI was ligased to vector phoA at 4°C, and was transformed into DH5α competent cells.

    Purification:

    Article Title: Multiplex CRISPR/Cas9-Based Genome Editing for Correction of Dystrophin Mutations that Cause Duchenne Muscular Dystrophy
    Article Snippet: .. PCR amplicons corresponding to the expected length of predicted translocations and only present in cells treated with sgRNA were purified (QIAGEN Gel Extraction kit) and analyzed by Sanger sequencing. .. mRNA analysis Immortalized myoblasts were differentiated into myofibers by replacing the growth medium with DMEM supplemented with 1% insulin-transferrin-selenium (Invitrogen #51500056) and 1% penicillin/streptomycin (Invitrogen #15140) for 5 days before the cells were trypsinized and collected.

    Article Title: Genetic transformation of the dinoflagellate chloroplast
    Article Snippet: .. PCR products were purified from excised gel pieces using the MinElute gel extraction kit (Qiagen). .. Some PCR products were directly sequenced after gel extraction whilst others were ligated into the pGEM-T Easy plasmid vector (Promega), following the manufacturer’s instructions.

    Article Title: Catalysts from synthetic genetic polymers
    Article Snippet: .. Bands of appropriate size were purified using a gel extraction kit (Qiagen, Netherlands) as per manufacturer’s instructions. .. Purified DNA was used as the polyclonal template for either sequencing library PCR (see below) or large scale preparative PCR (2ml) for generation of DNA templates for XNA synthesis.

    Article Title: Biogeography of Soil Bacterial Networks along a Gradient of Cropping Intensity
    Article Snippet: .. Crude DNA was then purified using a MinElute gel extraction kit (Qiagen, France) and quantified using a QuantiFluor staining kit (Promega, USA), prior to further investigations. .. PCR amplification and pyrosequencing of 16S rRNA gene sequences A 16S rRNA gene fragment targeting the V3-V4 regions to characterize bacterial diversity was amplified using the primers F479 (5′-CAGCMGCYGCNGTAANAC-3′) and R888 (5′-CCGYCAATTCMTTTRAGT-3′) with the method described previously .

    Polymerase Chain Reaction:

    Article Title: Multiplex CRISPR/Cas9-Based Genome Editing for Correction of Dystrophin Mutations that Cause Duchenne Muscular Dystrophy
    Article Snippet: .. PCR amplicons corresponding to the expected length of predicted translocations and only present in cells treated with sgRNA were purified (QIAGEN Gel Extraction kit) and analyzed by Sanger sequencing. .. mRNA analysis Immortalized myoblasts were differentiated into myofibers by replacing the growth medium with DMEM supplemented with 1% insulin-transferrin-selenium (Invitrogen #51500056) and 1% penicillin/streptomycin (Invitrogen #15140) for 5 days before the cells were trypsinized and collected.

    Article Title: Expression, purification of metallothionein genes from freshwater crab (Sinopotamon yangtsekiense) and development of an anti-metallothionein ELISA
    Article Snippet: .. Reaction was carried out with an initial denaturation step at 95°C for 5 min, followed by 30 cycles of 94°C for 30 s, 65°C for 30 s, 70°C for 1 min, and a final step of 70°C for 10 min. PCR fragments were detected by agarose gel electrophoresis, and extracted using DNA Gel Extraction Kit (Qiagen, Hilden, Germany). .. By T4 DNA ligase (New England Biolabs, Ipswich, MA), the extracted fragment digested with NcoI and BamHI was ligased to vector phoA at 4°C, and was transformed into DH5α competent cells.

    Article Title: Genetic transformation of the dinoflagellate chloroplast
    Article Snippet: .. PCR products were purified from excised gel pieces using the MinElute gel extraction kit (Qiagen). .. Some PCR products were directly sequenced after gel extraction whilst others were ligated into the pGEM-T Easy plasmid vector (Promega), following the manufacturer’s instructions.

    Article Title: HLA genotyping by next-generation sequencing of complementary DNA
    Article Snippet: .. The following cycling conditions were used: 1 cycle of 94 °C for 30 s, 40 cycles of 94 °C for 30 s, 65 °C for 30 s, and 68 °C for 1 min. After mixing each PCR product, gel extraction (range from 200 bp to 500 bp) was performed using the MinElute Gel Extraction Kit (Qiagen, Hilden, Germany). .. The concentration of the purified products was measured using the Quanti-iT™ dsDNA Assay Kit, Broad range.

    Gel Extraction:

    Article Title: Multiplex CRISPR/Cas9-Based Genome Editing for Correction of Dystrophin Mutations that Cause Duchenne Muscular Dystrophy
    Article Snippet: .. PCR amplicons corresponding to the expected length of predicted translocations and only present in cells treated with sgRNA were purified (QIAGEN Gel Extraction kit) and analyzed by Sanger sequencing. .. mRNA analysis Immortalized myoblasts were differentiated into myofibers by replacing the growth medium with DMEM supplemented with 1% insulin-transferrin-selenium (Invitrogen #51500056) and 1% penicillin/streptomycin (Invitrogen #15140) for 5 days before the cells were trypsinized and collected.

    Article Title: Field resistance to Fusarium oxysporum and Verticillium dahliae in transgenic cotton expressing the plant defensin NaD1
    Article Snippet: .. Amplified fragments were separated by gel electrophoresis, gel-purified (MinElute gel extraction kit, Qiagen), and directly sequenced using the second-round oligonucleotides (Macrogen, South Korea). .. Detection of NaD1 by immunoblot analysis Total soluble proteins were extracted from plant tissue in 50mM H2 SO4 plus 2% (w/v) polyvinylpyrrolidone (PVPP; 1g fresh weight tissue (1.2 ml buffer)–1 ) and insoluble material was removed by centrifugation (18,400 g , 10min).

    Article Title: Expression, purification of metallothionein genes from freshwater crab (Sinopotamon yangtsekiense) and development of an anti-metallothionein ELISA
    Article Snippet: .. Reaction was carried out with an initial denaturation step at 95°C for 5 min, followed by 30 cycles of 94°C for 30 s, 65°C for 30 s, 70°C for 1 min, and a final step of 70°C for 10 min. PCR fragments were detected by agarose gel electrophoresis, and extracted using DNA Gel Extraction Kit (Qiagen, Hilden, Germany). .. By T4 DNA ligase (New England Biolabs, Ipswich, MA), the extracted fragment digested with NcoI and BamHI was ligased to vector phoA at 4°C, and was transformed into DH5α competent cells.

    Article Title: Genetic transformation of the dinoflagellate chloroplast
    Article Snippet: .. PCR products were purified from excised gel pieces using the MinElute gel extraction kit (Qiagen). .. Some PCR products were directly sequenced after gel extraction whilst others were ligated into the pGEM-T Easy plasmid vector (Promega), following the manufacturer’s instructions.

    Article Title: Global Analysis of Transcription Factor-Binding Sites in Yeast Using ChIP-Seq
    Article Snippet: .. MinElute and QIAquick gel extraction kits (Qiagen). .. Illumina Genome Analyzer sequencer, with reagents, flowcell, and cluster station.

    Article Title: Catalysts from synthetic genetic polymers
    Article Snippet: .. Bands of appropriate size were purified using a gel extraction kit (Qiagen, Netherlands) as per manufacturer’s instructions. .. Purified DNA was used as the polyclonal template for either sequencing library PCR (see below) or large scale preparative PCR (2ml) for generation of DNA templates for XNA synthesis.

    Article Title: Biogeography of Soil Bacterial Networks along a Gradient of Cropping Intensity
    Article Snippet: .. Crude DNA was then purified using a MinElute gel extraction kit (Qiagen, France) and quantified using a QuantiFluor staining kit (Promega, USA), prior to further investigations. .. PCR amplification and pyrosequencing of 16S rRNA gene sequences A 16S rRNA gene fragment targeting the V3-V4 regions to characterize bacterial diversity was amplified using the primers F479 (5′-CAGCMGCYGCNGTAANAC-3′) and R888 (5′-CCGYCAATTCMTTTRAGT-3′) with the method described previously .

    Article Title: HLA genotyping by next-generation sequencing of complementary DNA
    Article Snippet: .. The following cycling conditions were used: 1 cycle of 94 °C for 30 s, 40 cycles of 94 °C for 30 s, 65 °C for 30 s, and 68 °C for 1 min. After mixing each PCR product, gel extraction (range from 200 bp to 500 bp) was performed using the MinElute Gel Extraction Kit (Qiagen, Hilden, Germany). .. The concentration of the purified products was measured using the Quanti-iT™ dsDNA Assay Kit, Broad range.

    Sequencing:

    Article Title: Multiplex CRISPR/Cas9-Based Genome Editing for Correction of Dystrophin Mutations that Cause Duchenne Muscular Dystrophy
    Article Snippet: .. PCR amplicons corresponding to the expected length of predicted translocations and only present in cells treated with sgRNA were purified (QIAGEN Gel Extraction kit) and analyzed by Sanger sequencing. .. mRNA analysis Immortalized myoblasts were differentiated into myofibers by replacing the growth medium with DMEM supplemented with 1% insulin-transferrin-selenium (Invitrogen #51500056) and 1% penicillin/streptomycin (Invitrogen #15140) for 5 days before the cells were trypsinized and collected.

    Staining:

    Article Title: Biogeography of Soil Bacterial Networks along a Gradient of Cropping Intensity
    Article Snippet: .. Crude DNA was then purified using a MinElute gel extraction kit (Qiagen, France) and quantified using a QuantiFluor staining kit (Promega, USA), prior to further investigations. .. PCR amplification and pyrosequencing of 16S rRNA gene sequences A 16S rRNA gene fragment targeting the V3-V4 regions to characterize bacterial diversity was amplified using the primers F479 (5′-CAGCMGCYGCNGTAANAC-3′) and R888 (5′-CCGYCAATTCMTTTRAGT-3′) with the method described previously .

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    Qiagen pcr purification kit
    Screening animals for ZFN-targeted mutation at the Nr2f2 locus (A) Tail <t>DNA</t> samples from pups born post-microinjection of custom ZFNs targeting exon 2 of the Nr2f2 locus were screened by <t>PCR</t> amplification with primers designed to amplify rat genomic fragments encompassing the ZFN-targeted site. Deviations from the expected product size of 360bp were inferred as genomic DNA from rats with mutations at the Nr2f2 locus. (B) Representative sequencing results from the PCR products shown in panel A detected a 15 bp deletion in the mRNA from mutant rats. Also represented are the corresponding translated peptide sequences as a result of the nucleotide variations.
    Pcr Purification Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 5966 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr purification kit/product/Qiagen
    Average 99 stars, based on 5966 article reviews
    Price from $9.99 to $1999.99
    pcr purification kit - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    88
    Qiagen rna affinity columns
    Functional and structural validation of newly discovered HIV-1 <t>RNA</t> motifs ( a ) Scheme for simultaneous deconvolution and structural analysis of a mixture of native sequence and U3 PK mutant genomes. (b) SHAPE profiles for the U3 PK pseudoknot bridging U3 and R. The experiment simultaneously probed a mixture of viruses with native sequence and mutant U3 PK <t>RNAs.</t> Secondary structure for the native sequence is shown as arcs below the y-axis intercept. Significant SHAPE reactivity differences are emphasized with yellow vertical lines (see Online Methods). (c) Direct growth competition and viral spread for U3 PK mutant and native sequence NL4-3 HIV-1 virions in Jurkat cells. Percentage of mutant in the initial inoculum is presented as a grey square at day 0. p24 levels correspond to the amount of HIV-1 capsid protein. ( d ) SHAPE profiles for the RT PK pseudoknot within the reverse transcriptase coding region. In this case, SHAPE data were obtained in separate experiments for each virus. ( e ) Viral spread and direct growth competition for RT PK mutant and native sequence NL4-3 HIV-1 virions in Jurkat cells. For the competition data, y-axes are shown on an expanded scale for clarity.
    Rna Affinity Columns, supplied by Qiagen, used in various techniques. Bioz Stars score: 88/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rna affinity columns/product/Qiagen
    Average 88 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    rna affinity columns - by Bioz Stars, 2020-07
    88/100 stars
      Buy from Supplier

    Image Search Results


    Screening animals for ZFN-targeted mutation at the Nr2f2 locus (A) Tail DNA samples from pups born post-microinjection of custom ZFNs targeting exon 2 of the Nr2f2 locus were screened by PCR amplification with primers designed to amplify rat genomic fragments encompassing the ZFN-targeted site. Deviations from the expected product size of 360bp were inferred as genomic DNA from rats with mutations at the Nr2f2 locus. (B) Representative sequencing results from the PCR products shown in panel A detected a 15 bp deletion in the mRNA from mutant rats. Also represented are the corresponding translated peptide sequences as a result of the nucleotide variations.

    Journal: Nature communications

    Article Title: Mutation within the hinge region of the transcription factor Nr2f2 attenuates salt-sensitive hypertension

    doi: 10.1038/ncomms7252

    Figure Lengend Snippet: Screening animals for ZFN-targeted mutation at the Nr2f2 locus (A) Tail DNA samples from pups born post-microinjection of custom ZFNs targeting exon 2 of the Nr2f2 locus were screened by PCR amplification with primers designed to amplify rat genomic fragments encompassing the ZFN-targeted site. Deviations from the expected product size of 360bp were inferred as genomic DNA from rats with mutations at the Nr2f2 locus. (B) Representative sequencing results from the PCR products shown in panel A detected a 15 bp deletion in the mRNA from mutant rats. Also represented are the corresponding translated peptide sequences as a result of the nucleotide variations.

    Article Snippet: Chromatin was treated with RNase A, proteinase K, and the DNA was column purified (PCR Purification kit, Qiagen).

    Techniques: Mutagenesis, Polymerase Chain Reaction, Amplification, Sequencing

    Figure 5. Nested PCR products of 345bp assessed by electrophoresis in a 1.2% Agarose gel. L = 100bp DNA ladder (Promega, UK), (2–6) CS-scars, (7) Positive control using placental tissue and (8) Negative control using nulliparous skin.

    Journal: Chimerism

    Article Title: Microchimeric fetal cells play a role in maternal wound healing after pregnancy

    doi: 10.4161/chim.28746

    Figure Lengend Snippet: Figure 5. Nested PCR products of 345bp assessed by electrophoresis in a 1.2% Agarose gel. L = 100bp DNA ladder (Promega, UK), (2–6) CS-scars, (7) Positive control using placental tissue and (8) Negative control using nulliparous skin.

    Article Snippet: The PCR product generated after the first reaction was then column purified using the Min Elute PCR purification kit (Qiagen) and DNA was eluted using 40 µl of DEPC-treated water.

    Techniques: Nested PCR, Electrophoresis, Agarose Gel Electrophoresis, Positive Control, Negative Control

    Schematic of the single-cell Quartz-Seq and Quartz-Chip methods . All of the steps of the whole-transcript amplification were executed in a single PCR tube. The first-strand cDNA was synthesized using the reverse transcription (RT) primer, which contains oligo-dT24, the T7 promoter (T7) and the PCR target region (M) sequences. After the first-strand synthesis, the majority of the RT primer was digested by exonuclease I, although it was not possible to eliminate the RT primer completely using this procedure. A poly-A tail was then added to the 3' ends of the first-strand cDNA and to any surviving RT primer. After the second-strand synthesis with the tagging primer, the resulting cDNA and the byproducts from the surviving primers contained the whole-transcript amplification (WTA) adaptor sequences, which include the RT primer sequence and the tagging primer sequence. These DNAs were used for the suppression PCR, which used the suppression PCR primer. Enrichment of the short DNA fragments, such as the byproducts, was suppressed. After the enrichment, the high-quality cDNA, which did not contain any byproducts, was obtained. The amplified cDNAs then had the T7 promoter sequence at the 3' ends of the DNA. These cDNAs were used for the Illumina sequencing and microarray experiments.

    Journal: Genome Biology

    Article Title: Quartz-Seq: a highly reproducible and sensitive single-cell RNA sequencing method, reveals non-genetic gene-expression heterogeneity

    doi: 10.1186/gb-2013-14-4-r31

    Figure Lengend Snippet: Schematic of the single-cell Quartz-Seq and Quartz-Chip methods . All of the steps of the whole-transcript amplification were executed in a single PCR tube. The first-strand cDNA was synthesized using the reverse transcription (RT) primer, which contains oligo-dT24, the T7 promoter (T7) and the PCR target region (M) sequences. After the first-strand synthesis, the majority of the RT primer was digested by exonuclease I, although it was not possible to eliminate the RT primer completely using this procedure. A poly-A tail was then added to the 3' ends of the first-strand cDNA and to any surviving RT primer. After the second-strand synthesis with the tagging primer, the resulting cDNA and the byproducts from the surviving primers contained the whole-transcript amplification (WTA) adaptor sequences, which include the RT primer sequence and the tagging primer sequence. These DNAs were used for the suppression PCR, which used the suppression PCR primer. Enrichment of the short DNA fragments, such as the byproducts, was suppressed. After the enrichment, the high-quality cDNA, which did not contain any byproducts, was obtained. The amplified cDNAs then had the T7 promoter sequence at the 3' ends of the DNA. These cDNAs were used for the Illumina sequencing and microarray experiments.

    Article Snippet: The amplified cDNA was purified using a PCR purification column (MinElute; Qiagen) or a PCR purification bead system (Agencourt AMPure XP; Beckman Coulter Inc., Brea, CA, USA).

    Techniques: Chromatin Immunoprecipitation, Amplification, Polymerase Chain Reaction, Synthesized, Sequencing, Microarray

    Functional and structural validation of newly discovered HIV-1 RNA motifs ( a ) Scheme for simultaneous deconvolution and structural analysis of a mixture of native sequence and U3 PK mutant genomes. (b) SHAPE profiles for the U3 PK pseudoknot bridging U3 and R. The experiment simultaneously probed a mixture of viruses with native sequence and mutant U3 PK RNAs. Secondary structure for the native sequence is shown as arcs below the y-axis intercept. Significant SHAPE reactivity differences are emphasized with yellow vertical lines (see Online Methods). (c) Direct growth competition and viral spread for U3 PK mutant and native sequence NL4-3 HIV-1 virions in Jurkat cells. Percentage of mutant in the initial inoculum is presented as a grey square at day 0. p24 levels correspond to the amount of HIV-1 capsid protein. ( d ) SHAPE profiles for the RT PK pseudoknot within the reverse transcriptase coding region. In this case, SHAPE data were obtained in separate experiments for each virus. ( e ) Viral spread and direct growth competition for RT PK mutant and native sequence NL4-3 HIV-1 virions in Jurkat cells. For the competition data, y-axes are shown on an expanded scale for clarity.

    Journal: Nature methods

    Article Title: RNA motif discovery by SHAPE and mutational profiling (SHAPE-MaP)

    doi: 10.1038/nmeth.3029

    Figure Lengend Snippet: Functional and structural validation of newly discovered HIV-1 RNA motifs ( a ) Scheme for simultaneous deconvolution and structural analysis of a mixture of native sequence and U3 PK mutant genomes. (b) SHAPE profiles for the U3 PK pseudoknot bridging U3 and R. The experiment simultaneously probed a mixture of viruses with native sequence and mutant U3 PK RNAs. Secondary structure for the native sequence is shown as arcs below the y-axis intercept. Significant SHAPE reactivity differences are emphasized with yellow vertical lines (see Online Methods). (c) Direct growth competition and viral spread for U3 PK mutant and native sequence NL4-3 HIV-1 virions in Jurkat cells. Percentage of mutant in the initial inoculum is presented as a grey square at day 0. p24 levels correspond to the amount of HIV-1 capsid protein. ( d ) SHAPE profiles for the RT PK pseudoknot within the reverse transcriptase coding region. In this case, SHAPE data were obtained in separate experiments for each virus. ( e ) Viral spread and direct growth competition for RT PK mutant and native sequence NL4-3 HIV-1 virions in Jurkat cells. For the competition data, y-axes are shown on an expanded scale for clarity.

    Article Snippet: Following modification, RNAs were isolated using either RNA affinity columns (RNeasy MinElute; Qiagen) or G-50 spin columns (GE Healthcare).

    Techniques: Functional Assay, Sequencing, Mutagenesis