millipore assay buffer  (Millipore)


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    Structured Review

    Millipore millipore assay buffer
    Millipore Assay Buffer, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/millipore assay buffer/product/Millipore
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    millipore assay buffer - by Bioz Stars, 2020-04
    94/100 stars

    Related Products / Commonly Used Together

    nasosorption matrix
    1c22‐mp
    isotyping kit

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    Related Articles

    Centrifugation:

    Article Title: Biphasic activation of complement and fibrinolysis during the human nasal allergic response
    Article Snippet: .. Fluid was eluted from the nasosorption matrix using 300 μL of Millipore assay buffer (Millipore, Billerica, Mass) and centrifugation at 16000 g for 20 minutes, at 4°C. .. Samples for measurement of TF were eluted using the same protocol, with the exception that Tris-buffered saline replaced Millipore assay buffer.

    Article Title: Biphasic activation of complement and fibrinolysis during the human nasal allergic response
    Article Snippet: .. Fluid was eluted from the nasosorption matrix using 300 μL of Millipore assay buffer (Millipore, Billerica, Mass) and centrifugation at 16000g for 20 minutes, at 4°C. .. Samples for measurement of TF were eluted using the same protocol, with the exception that Tris-buffered saline replaced Millipore assay buffer.

    other:

    Article Title: Complex sex-biased antibody responses: estrogen receptors bind estrogen response elements centered within immunoglobulin heavy chain gene enhancers
    Article Snippet: The isotyping kit and procedures described above were used, except that supernatants were first mixed and diluted 1:5 in Millipore assay buffer.

    Sampling:

    Article Title: Biphasic activation of complement and fibrinolysis during the human nasal allergic response
    Article Snippet: Paragraph title: Sampling ... Fluid was eluted from the nasosorption matrix using 300 μL of Millipore assay buffer (Millipore, Billerica, Mass) and centrifugation at 16000 g for 20 minutes, at 4°C.

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  • 95
    Millipore hat assay buffer
    Inhibitory effects of TA on <t>HAT</t> activity. (A) A colorimetric assay was performed to measure HAT activity in the presence of TA at the indicated concentrations. The results are presented as percentages relative to the control sample, which was incubated without TA (left panel). The IC 50 value of TA inhibition of HAT activity was calculated using Prism software (right panel). (B) An in vitro HAT assay using autoradiography was performed to evaluate the effect of the anti-HAT activity of TA on the acetylation of a synthetic H4 peptide by a <t>HeLa</t> NE. The arrow indicates histone H4 (left panel). The autoradiography was quantified using Image J software (right panel). The values presented are the means ± SD of two independent experiments. Means with different superscript letters are significantly different, p
    Hat Assay Buffer, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hat assay buffer/product/Millipore
    Average 95 stars, based on 15 article reviews
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    99
    Millipore sds loading buffer
    RanBP3L inhibits BMP-induced transcriptional responses. (A) Schematic diagram of RanBP3L and RanBP3. RanBP3L contains a Ran-binding domain (RanBD) with 71% identity to that of RanBP3. (B) RanBP3L binds with RanGTP. HEK293T cells were cotransfected with Myc-RanBP3L and FLAG-RanQ69L. RanBP3L was immunoprecipitated (IP) with anti-Myc antibody and then subjected to <t>SDS-PAGE</t> and Western blotting to detect RanBP3L-bound RanQ69L. WCL, whole-cell lysate. (C to E) RanBP3L inhibits BMP2-induced Id1-luc reporter activity. C3H10T1/2 (C), C2C12 (D), and ATDC5 cells (E) were transfected with RanBP3L, PPM1A, or RanBP3 together with Id1-luc reporter plasmids. BMP2 treatment and luciferase assays were done as described in Materials and Methods. RLU, relative light units. (F) RanBP3L does not affect TGF-β-induced SBE-luc reporter activity in HaCaT cells. HaCaT cells were transfected with RanBP3 or RanBP3L together with the SBE-luc reporter plasmid and then treated with 2 ng/ml TGF-β for 12 h. The experiment was carried out as described above for panels C to E. (G and H) Stable expression of hRanBP3L in C2C12 cells decreases mRNA levels of Id1 (G) and Id2 (H). Cells were treated with LDN193189 (BMP type I receptor inhibitor) (LDN) or BMP2 (50 ng/ml) for 6 h, and total RNA was extracted for qRT-PCR analysis. (I) RanBP3L knockdown efficacy was measured in C2C12 cells. C2C12 cells were transfected with RanBP3L siRNAs or control siRNA (siCTR), and total RNA was extracted for qRT-PCR analysis. (J) Knockdown of RanBP3L enhances BMP2-induced Id1-luc reporter activity. C2C12 cells were transfected with RanBP3L siRNAs or control siRNA, together with the Id1-luc reporter plasmid. (K and L) Knockdown of RanBP3L increases Id1 mRNA expression (K) and Id2 mRNA expression (L). C2C12 cells were transfected with the indicated siRNAs and treated with BMP2 (50 ng/ml) for 6 h or not treated.
    Sds Loading Buffer, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 172 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 172 article reviews
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    91
    Millipore n2b27 media
    (A) Experimental analysis of cell viability from the six-chamber microfluidic device where cells were washed at 0, 5, 20, 60, 180, and 720 minute intervals. Results include both those for cells cultured with the serum-containing medium and cells cultured with the defined, <t>N2B27</t> serum-deficient medium. The data are presented as the means of three separate experiments, with the error bars representing the standard error of the mean. (B) Twelve hour time course of the viability of cells under each of the washing regimes, with a three hour initial attachment period ( i.e. , the cells are exposed to different washing regimes at 3 h and thereafter).
    N2b27 Media, supplied by Millipore, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 91 stars, based on 2 article reviews
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    96
    Millipore t75 flask
    PIA and Per cause nanovesicle release in vivo . ( a ) Athymic mice bearing H157 xenografts were given a single i.p. injection of vehicle or 90 mg/kg P5. After 1 h, cardiac puncture was performed; plasma was isolated and subjected to differential centrifugation as in the Materials and methods section. Shown are the 100 000 × g plasma pellets from two vehicle (V), or three P5-treated (P) mice. ( b ) PIA and Per-induced nanovesicles are internalized. Photomicrographs of H157 cells incubated for 72 h with nanovesicle pellet from 1 h DMSO or PIA, or Per-treated EGFR-GFP H157 donor cells. ( c ) The 100 000 × g media pellet from treated donor cells causes dose-dependent inhibition of proliferation in recipient cells. Shown is the growth of recipient cells compared with untreated control after 72-h incubation with 100 000 × g media pellet from 1 h PIA or Per-treated EGFR-GFP H157 donor cells. The amount of pellet added to the recipient cells is reflected in the legend. × 1, media pellet from one <t>T75</t> donor flask, × 3, media pellet from three T75 donor flasks. ( d ) The 100 000 × g media pellet from 1 h PIA- or Per-treated cells increases ceramide (red color) in recipient cells that is blocked by a SMPD3 inhibitor
    T75 Flask, supplied by Millipore, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Inhibitory effects of TA on HAT activity. (A) A colorimetric assay was performed to measure HAT activity in the presence of TA at the indicated concentrations. The results are presented as percentages relative to the control sample, which was incubated without TA (left panel). The IC 50 value of TA inhibition of HAT activity was calculated using Prism software (right panel). (B) An in vitro HAT assay using autoradiography was performed to evaluate the effect of the anti-HAT activity of TA on the acetylation of a synthetic H4 peptide by a HeLa NE. The arrow indicates histone H4 (left panel). The autoradiography was quantified using Image J software (right panel). The values presented are the means ± SD of two independent experiments. Means with different superscript letters are significantly different, p

    Journal: Molecular Metabolism

    Article Title: Tannic acid, a novel histone acetyltransferase inhibitor, prevents non-alcoholic fatty liver disease both in vivo and in vitro model

    doi: 10.1016/j.molmet.2018.11.001

    Figure Lengend Snippet: Inhibitory effects of TA on HAT activity. (A) A colorimetric assay was performed to measure HAT activity in the presence of TA at the indicated concentrations. The results are presented as percentages relative to the control sample, which was incubated without TA (left panel). The IC 50 value of TA inhibition of HAT activity was calculated using Prism software (right panel). (B) An in vitro HAT assay using autoradiography was performed to evaluate the effect of the anti-HAT activity of TA on the acetylation of a synthetic H4 peptide by a HeLa NE. The arrow indicates histone H4 (left panel). The autoradiography was quantified using Image J software (right panel). The values presented are the means ± SD of two independent experiments. Means with different superscript letters are significantly different, p

    Article Snippet: For autoradiography-based in vitro HAT activity assays, HeLa cell nuclear extract was incubated with HAT assay buffer (50 mM HEPES, pH 8.0; 10% glycerol; 1 mM DTT; 1 mM phenylmethylsulfonyl fluoride; and 10 mM sodium butyrate), 1 μL [3 H] acetyl-CoA, and 5 μg of biotinylated-H4 peptide (Millipore, Billerica, MA, USA) along with TA at the indicated concentrations at 30 °C for 1 h. The reactions were stopped by adding 5 × sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS–PAGE) sample buffer and samples were separated on 15% SDS–PAGE gels, followed by autoradiographic analysis.

    Techniques: HAT Assay, Activity Assay, Colorimetric Assay, Incubation, Inhibition, Software, In Vitro, Autoradiography

    RanBP3L inhibits BMP-induced transcriptional responses. (A) Schematic diagram of RanBP3L and RanBP3. RanBP3L contains a Ran-binding domain (RanBD) with 71% identity to that of RanBP3. (B) RanBP3L binds with RanGTP. HEK293T cells were cotransfected with Myc-RanBP3L and FLAG-RanQ69L. RanBP3L was immunoprecipitated (IP) with anti-Myc antibody and then subjected to SDS-PAGE and Western blotting to detect RanBP3L-bound RanQ69L. WCL, whole-cell lysate. (C to E) RanBP3L inhibits BMP2-induced Id1-luc reporter activity. C3H10T1/2 (C), C2C12 (D), and ATDC5 cells (E) were transfected with RanBP3L, PPM1A, or RanBP3 together with Id1-luc reporter plasmids. BMP2 treatment and luciferase assays were done as described in Materials and Methods. RLU, relative light units. (F) RanBP3L does not affect TGF-β-induced SBE-luc reporter activity in HaCaT cells. HaCaT cells were transfected with RanBP3 or RanBP3L together with the SBE-luc reporter plasmid and then treated with 2 ng/ml TGF-β for 12 h. The experiment was carried out as described above for panels C to E. (G and H) Stable expression of hRanBP3L in C2C12 cells decreases mRNA levels of Id1 (G) and Id2 (H). Cells were treated with LDN193189 (BMP type I receptor inhibitor) (LDN) or BMP2 (50 ng/ml) for 6 h, and total RNA was extracted for qRT-PCR analysis. (I) RanBP3L knockdown efficacy was measured in C2C12 cells. C2C12 cells were transfected with RanBP3L siRNAs or control siRNA (siCTR), and total RNA was extracted for qRT-PCR analysis. (J) Knockdown of RanBP3L enhances BMP2-induced Id1-luc reporter activity. C2C12 cells were transfected with RanBP3L siRNAs or control siRNA, together with the Id1-luc reporter plasmid. (K and L) Knockdown of RanBP3L increases Id1 mRNA expression (K) and Id2 mRNA expression (L). C2C12 cells were transfected with the indicated siRNAs and treated with BMP2 (50 ng/ml) for 6 h or not treated.

    Journal: Molecular and Cellular Biology

    Article Title: Nuclear Export of Smads by RanBP3L Regulates Bone Morphogenetic Protein Signaling and Mesenchymal Stem Cell Differentiation

    doi: 10.1128/MCB.00121-15

    Figure Lengend Snippet: RanBP3L inhibits BMP-induced transcriptional responses. (A) Schematic diagram of RanBP3L and RanBP3. RanBP3L contains a Ran-binding domain (RanBD) with 71% identity to that of RanBP3. (B) RanBP3L binds with RanGTP. HEK293T cells were cotransfected with Myc-RanBP3L and FLAG-RanQ69L. RanBP3L was immunoprecipitated (IP) with anti-Myc antibody and then subjected to SDS-PAGE and Western blotting to detect RanBP3L-bound RanQ69L. WCL, whole-cell lysate. (C to E) RanBP3L inhibits BMP2-induced Id1-luc reporter activity. C3H10T1/2 (C), C2C12 (D), and ATDC5 cells (E) were transfected with RanBP3L, PPM1A, or RanBP3 together with Id1-luc reporter plasmids. BMP2 treatment and luciferase assays were done as described in Materials and Methods. RLU, relative light units. (F) RanBP3L does not affect TGF-β-induced SBE-luc reporter activity in HaCaT cells. HaCaT cells were transfected with RanBP3 or RanBP3L together with the SBE-luc reporter plasmid and then treated with 2 ng/ml TGF-β for 12 h. The experiment was carried out as described above for panels C to E. (G and H) Stable expression of hRanBP3L in C2C12 cells decreases mRNA levels of Id1 (G) and Id2 (H). Cells were treated with LDN193189 (BMP type I receptor inhibitor) (LDN) or BMP2 (50 ng/ml) for 6 h, and total RNA was extracted for qRT-PCR analysis. (I) RanBP3L knockdown efficacy was measured in C2C12 cells. C2C12 cells were transfected with RanBP3L siRNAs or control siRNA (siCTR), and total RNA was extracted for qRT-PCR analysis. (J) Knockdown of RanBP3L enhances BMP2-induced Id1-luc reporter activity. C2C12 cells were transfected with RanBP3L siRNAs or control siRNA, together with the Id1-luc reporter plasmid. (K and L) Knockdown of RanBP3L increases Id1 mRNA expression (K) and Id2 mRNA expression (L). C2C12 cells were transfected with the indicated siRNAs and treated with BMP2 (50 ng/ml) for 6 h or not treated.

    Article Snippet: After several washes, precipitated proteins were eluted in SDS loading buffer, separated by SDS-PAGE, transferred onto polyvinylidene difluoride (PVDF) membranes (Millipore), and detected by Western blotting with appropriate antibodies.

    Techniques: Binding Assay, Immunoprecipitation, SDS Page, Western Blot, Activity Assay, Transfection, Luciferase, Plasmid Preparation, Expressing, Quantitative RT-PCR

    (A) Experimental analysis of cell viability from the six-chamber microfluidic device where cells were washed at 0, 5, 20, 60, 180, and 720 minute intervals. Results include both those for cells cultured with the serum-containing medium and cells cultured with the defined, N2B27 serum-deficient medium. The data are presented as the means of three separate experiments, with the error bars representing the standard error of the mean. (B) Twelve hour time course of the viability of cells under each of the washing regimes, with a three hour initial attachment period ( i.e. , the cells are exposed to different washing regimes at 3 h and thereafter).

    Journal: Molecular bioSystems

    Article Title: Computational model and microfluidic platform for the investigation of paracrine and autocrine signaling in mouse embryonic stem cells

    doi: 10.1039/b905602e

    Figure Lengend Snippet: (A) Experimental analysis of cell viability from the six-chamber microfluidic device where cells were washed at 0, 5, 20, 60, 180, and 720 minute intervals. Results include both those for cells cultured with the serum-containing medium and cells cultured with the defined, N2B27 serum-deficient medium. The data are presented as the means of three separate experiments, with the error bars representing the standard error of the mean. (B) Twelve hour time course of the viability of cells under each of the washing regimes, with a three hour initial attachment period ( i.e. , the cells are exposed to different washing regimes at 3 h and thereafter).

    Article Snippet: N2B27 media supplemented with 10 ng mL−1 LIF (ESGRO, Millipore) and 10 ng mL−1 BMP4 (R & D Systems) was used and the cells were passaged every 2–4 days using either enzyme-free cell dissociation buffer (Invitrogen) or 0.05% trypsin (Invitrogen).

    Techniques: Cell Culture

    PIA and Per cause nanovesicle release in vivo . ( a ) Athymic mice bearing H157 xenografts were given a single i.p. injection of vehicle or 90 mg/kg P5. After 1 h, cardiac puncture was performed; plasma was isolated and subjected to differential centrifugation as in the Materials and methods section. Shown are the 100 000 × g plasma pellets from two vehicle (V), or three P5-treated (P) mice. ( b ) PIA and Per-induced nanovesicles are internalized. Photomicrographs of H157 cells incubated for 72 h with nanovesicle pellet from 1 h DMSO or PIA, or Per-treated EGFR-GFP H157 donor cells. ( c ) The 100 000 × g media pellet from treated donor cells causes dose-dependent inhibition of proliferation in recipient cells. Shown is the growth of recipient cells compared with untreated control after 72-h incubation with 100 000 × g media pellet from 1 h PIA or Per-treated EGFR-GFP H157 donor cells. The amount of pellet added to the recipient cells is reflected in the legend. × 1, media pellet from one T75 donor flask, × 3, media pellet from three T75 donor flasks. ( d ) The 100 000 × g media pellet from 1 h PIA- or Per-treated cells increases ceramide (red color) in recipient cells that is blocked by a SMPD3 inhibitor

    Journal: Cell Death & Disease

    Article Title: Ceramide mediates nanovesicle shedding and cell death in response to phosphatidylinositol ether lipid analogs and perifosine

    doi: 10.1038/cddis.2012.72

    Figure Lengend Snippet: PIA and Per cause nanovesicle release in vivo . ( a ) Athymic mice bearing H157 xenografts were given a single i.p. injection of vehicle or 90 mg/kg P5. After 1 h, cardiac puncture was performed; plasma was isolated and subjected to differential centrifugation as in the Materials and methods section. Shown are the 100 000 × g plasma pellets from two vehicle (V), or three P5-treated (P) mice. ( b ) PIA and Per-induced nanovesicles are internalized. Photomicrographs of H157 cells incubated for 72 h with nanovesicle pellet from 1 h DMSO or PIA, or Per-treated EGFR-GFP H157 donor cells. ( c ) The 100 000 × g media pellet from treated donor cells causes dose-dependent inhibition of proliferation in recipient cells. Shown is the growth of recipient cells compared with untreated control after 72-h incubation with 100 000 × g media pellet from 1 h PIA or Per-treated EGFR-GFP H157 donor cells. The amount of pellet added to the recipient cells is reflected in the legend. × 1, media pellet from one T75 donor flask, × 3, media pellet from three T75 donor flasks. ( d ) The 100 000 × g media pellet from 1 h PIA- or Per-treated cells increases ceramide (red color) in recipient cells that is blocked by a SMPD3 inhibitor

    Article Snippet: Cell culture media concentration and fractionation (nanovesicle isolation) For the initial assessment of cell culture media, media from a T75 flask were concentrated (∼30 × reduction in volume) using a Centricon Ultracel YM-10 filter unit (Millipore), and immunoblot analysis was performed on 1/10 of the remaining media volume.

    Techniques: In Vivo, Mouse Assay, Injection, Isolation, Centrifugation, Incubation, Inhibition