Structured Review

Millipore milli q water
NIRS-based discrimination of <t>Milli-Q</t> water and cis-syn T
Milli Q Water, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 84 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Images

1) Product Images from "Detection of UV-induced cyclobutane pyrimidine dimers by near-infrared spectroscopy and aquaphotomics"

Article Title: Detection of UV-induced cyclobutane pyrimidine dimers by near-infrared spectroscopy and aquaphotomics

Journal: Scientific Reports

doi: 10.1038/srep11808

NIRS-based discrimination of Milli-Q water and cis-syn T
Figure Legend Snippet: NIRS-based discrimination of Milli-Q water and cis-syn T

Techniques Used:

2) Product Images from "Chemical and Electrochemical Synthesis of Polypyrrole Using Carrageenan as a Dopant: Polypyrrole/Multi-Walled Carbon Nanotube Nanocomposites"

Article Title: Chemical and Electrochemical Synthesis of Polypyrrole Using Carrageenan as a Dopant: Polypyrrole/Multi-Walled Carbon Nanotube Nanocomposites

Journal: Polymers

doi: 10.3390/polym10060632

( a ) The UV-Vis absorption of PPy-IC, PPy-KC, PPy-IC/MWNT and PPy-KC/MWNT composite solutions at a concentration of 1.0 mg/mL in Milli-Q water; ( b ) Stability of PPy-IC, PPy-KC, PPy-IC/MWNT and PPy-KC/MWNT composite solutions at a concentration of 2.0 mg/mL in Milli-Q water.
Figure Legend Snippet: ( a ) The UV-Vis absorption of PPy-IC, PPy-KC, PPy-IC/MWNT and PPy-KC/MWNT composite solutions at a concentration of 1.0 mg/mL in Milli-Q water; ( b ) Stability of PPy-IC, PPy-KC, PPy-IC/MWNT and PPy-KC/MWNT composite solutions at a concentration of 2.0 mg/mL in Milli-Q water.

Techniques Used: Concentration Assay

( a ) UV-Vis absorption spectra of PPy-IC at different concentrations in Milli-Q water and ( b ) the absorbance at 970 nm of different concentrations of PPy-IC.
Figure Legend Snippet: ( a ) UV-Vis absorption spectra of PPy-IC at different concentrations in Milli-Q water and ( b ) the absorbance at 970 nm of different concentrations of PPy-IC.

Techniques Used:

3) Product Images from "The Impacts of Phosphorus Deficiency on the Photosynthetic Electron Transport Chain 1The Impacts of Phosphorus Deficiency on the Photosynthetic Electron Transport Chain 1 [OPEN]"

Article Title: The Impacts of Phosphorus Deficiency on the Photosynthetic Electron Transport Chain 1The Impacts of Phosphorus Deficiency on the Photosynthetic Electron Transport Chain 1 [OPEN]

Journal: Plant Physiology

doi: 10.1104/pp.17.01624

OJIP transients recorded from the youngest, fully expanded barley leaves. A, Main plot. Transients were recorded just before P resupply at 21 DAP. The inset shows transients recorded 7 d after P resupply at 28 DAP. The slope of the quenching curve was calculated between the two dashed vertical lines (between 2 and 10 s). B, Transients recorded for P-deficient leaves immersed in Milli-Q water (P deficient) or P solution (P resupply) for 60 min. All transients were averaged (A, n = 5; B, n = 4, each with more than four technical replicates) and doubled normalized between F 0 and F m . A.U., Arbitrary units.
Figure Legend Snippet: OJIP transients recorded from the youngest, fully expanded barley leaves. A, Main plot. Transients were recorded just before P resupply at 21 DAP. The inset shows transients recorded 7 d after P resupply at 28 DAP. The slope of the quenching curve was calculated between the two dashed vertical lines (between 2 and 10 s). B, Transients recorded for P-deficient leaves immersed in Milli-Q water (P deficient) or P solution (P resupply) for 60 min. All transients were averaged (A, n = 5; B, n = 4, each with more than four technical replicates) and doubled normalized between F 0 and F m . A.U., Arbitrary units.

Techniques Used:

4) Product Images from "Mix‐ ‐Read Determination of Mercury(II) at Trace Levels with Hybrid Mesoporous Silica Materials Incorporating Fluorescent Probes by a Simple Mix‐ ‐Load Technique"

Article Title: Mix‐ ‐Read Determination of Mercury(II) at Trace Levels with Hybrid Mesoporous Silica Materials Incorporating Fluorescent Probes by a Simple Mix‐ ‐Load Technique

Journal: ChemistryOpen

doi: 10.1002/open.201800111

a) Intensity of fluorescence registered at λ =538 nm ( λ ex =490 nm) of xPRO‐SBA‐ I suspended in Milli‐Q water (0.11 mg mL −1 ; pH 7) in the presence of 6 μ m of Mn II , Co II , Zn II , Cd II , Mg II , Fe II , Cu II , Ni II , Hg II , and Ag I . b) Fluorescence enhancement in the corresponding suspensions as a function of the concentration of Ag I , Hg II , Ni II , and Cu II .
Figure Legend Snippet: a) Intensity of fluorescence registered at λ =538 nm ( λ ex =490 nm) of xPRO‐SBA‐ I suspended in Milli‐Q water (0.11 mg mL −1 ; pH 7) in the presence of 6 μ m of Mn II , Co II , Zn II , Cd II , Mg II , Fe II , Cu II , Ni II , Hg II , and Ag I . b) Fluorescence enhancement in the corresponding suspensions as a function of the concentration of Ag I , Hg II , Ni II , and Cu II .

Techniques Used: Fluorescence, Concentration Assay

a) Fluorescence intensity at λ =540 nm of I in water (1.7 μ m in H 2 O at pH 7; λ ex =490 nm) registered as a function of time while stirring the solution in a cuvette in the absence (blue line) and presence (black line) of Hg II (500 ppb). Red lines correspond to fits of the data to a first‐order kinetic reaction model. b) Absorption spectra of I (1.7 μ m , Milli‐Q water, pH 7) in the absence and in the presence of Hg II (0–3 ppm). Inset: Photograph showing the color changing from pink to yellow; see also Figure S1 for a larger version. From left to right, c Hg =0, 0.1, and 0.8 ppm. c) Corresponding fluorescence spectra ( λ ex =490 nm). d) Normalized excitation (d.1, top; λ em =564 nm) and emission (d.2, bottom; λ ex =490 nm) titration spectra. e) Plot of fluorescence enhancement ratio, Δ F / F 0 , that is, ( F − F 0 )/ F 0 , registered at λ =538 nm ( λ ex =490 nm) as a function of Hg II added. Inset: Photograph showing change in fluorescence under UV light ( λ ex =365 nm) in the absence and in the presence of Hg II (2 ppm).
Figure Legend Snippet: a) Fluorescence intensity at λ =540 nm of I in water (1.7 μ m in H 2 O at pH 7; λ ex =490 nm) registered as a function of time while stirring the solution in a cuvette in the absence (blue line) and presence (black line) of Hg II (500 ppb). Red lines correspond to fits of the data to a first‐order kinetic reaction model. b) Absorption spectra of I (1.7 μ m , Milli‐Q water, pH 7) in the absence and in the presence of Hg II (0–3 ppm). Inset: Photograph showing the color changing from pink to yellow; see also Figure S1 for a larger version. From left to right, c Hg =0, 0.1, and 0.8 ppm. c) Corresponding fluorescence spectra ( λ ex =490 nm). d) Normalized excitation (d.1, top; λ em =564 nm) and emission (d.2, bottom; λ ex =490 nm) titration spectra. e) Plot of fluorescence enhancement ratio, Δ F / F 0 , that is, ( F − F 0 )/ F 0 , registered at λ =538 nm ( λ ex =490 nm) as a function of Hg II added. Inset: Photograph showing change in fluorescence under UV light ( λ ex =365 nm) in the absence and in the presence of Hg II (2 ppm).

Techniques Used: Fluorescence, Titration

a) Absorption spectrum (red) and fluorescence excitation (solid black; λ em =564 nm) and emission (dashed black; λ ex =490 nm) spectra of xPRO‐SBA‐ I′ in Milli‐Q water (0.11 mg mL −1 ; pH 7). b) Normalized absorption spectra of a suspension of xPRO‐SBA‐ I′ in the presence of various amounts of Hg II , increasing from red to blue. c) Fluorescence titration spectra ( λ ex =490 nm) of xPRO‐SBA‐ I′ suspended in Milli‐Q water (0.11 mg mL −1 ; pH 7) upon the addition of Hg II . Inset: Corresponding fluorescence excitation spectra ( λ em =564 nm). d) Corresponding fluorescence enhancement ratio (Δ F / F 0 ) registered at λ =538 nm ( λ ex =490 nm) for xPRO‐SBA‐ I′ suspended in Milli‐Q water (0.11 mg mL −1 ; pH 7) in the presence of increasing amounts of Hg II . Inset: Magnification of the low‐concentration range.
Figure Legend Snippet: a) Absorption spectrum (red) and fluorescence excitation (solid black; λ em =564 nm) and emission (dashed black; λ ex =490 nm) spectra of xPRO‐SBA‐ I′ in Milli‐Q water (0.11 mg mL −1 ; pH 7). b) Normalized absorption spectra of a suspension of xPRO‐SBA‐ I′ in the presence of various amounts of Hg II , increasing from red to blue. c) Fluorescence titration spectra ( λ ex =490 nm) of xPRO‐SBA‐ I′ suspended in Milli‐Q water (0.11 mg mL −1 ; pH 7) upon the addition of Hg II . Inset: Corresponding fluorescence excitation spectra ( λ em =564 nm). d) Corresponding fluorescence enhancement ratio (Δ F / F 0 ) registered at λ =538 nm ( λ ex =490 nm) for xPRO‐SBA‐ I′ suspended in Milli‐Q water (0.11 mg mL −1 ; pH 7) in the presence of increasing amounts of Hg II . Inset: Magnification of the low‐concentration range.

Techniques Used: Fluorescence, Titration, Concentration Assay

a) Absorption spectra (solid lines) and fluorescence excitation spectra ( λ em =564 nm; dashed lines) of BODIPY dye I ( c= 1.6 μ m ) in MeCN (black) and in Milli‐Q water at pH 7 (red). b) Corresponding fluorescence emission spectra ( λ ex =490 nm) of I in MeCN (black) and Milli‐Q water at pH 7 (red).
Figure Legend Snippet: a) Absorption spectra (solid lines) and fluorescence excitation spectra ( λ em =564 nm; dashed lines) of BODIPY dye I ( c= 1.6 μ m ) in MeCN (black) and in Milli‐Q water at pH 7 (red). b) Corresponding fluorescence emission spectra ( λ ex =490 nm) of I in MeCN (black) and Milli‐Q water at pH 7 (red).

Techniques Used: Fluorescence

a) Absorption (solid red line) and fluorescence excitation ( λ em =564 nm) and emission spectra ( λ ex =490 nm; solid black lines) of xPRO‐SBA‐ I in Milli‐Q water (0.11 mg mL −1 , pH 7). b) Fluorescence intensity registered at λ =538 nm ( λ ex =490 nm) of xPRO‐SBA‐ I in Milli‐Q water (0.11 mg mL −1 , pH 7) as a function of time in the absence (blue line) and presence (black line) of Hg II (500 ppb). Note that a suspension of blank xPRO‐SBA (containing no dye) at the respective concentration was used to correct for scattered light in the absorption spectrum.
Figure Legend Snippet: a) Absorption (solid red line) and fluorescence excitation ( λ em =564 nm) and emission spectra ( λ ex =490 nm; solid black lines) of xPRO‐SBA‐ I in Milli‐Q water (0.11 mg mL −1 , pH 7). b) Fluorescence intensity registered at λ =538 nm ( λ ex =490 nm) of xPRO‐SBA‐ I in Milli‐Q water (0.11 mg mL −1 , pH 7) as a function of time in the absence (blue line) and presence (black line) of Hg II (500 ppb). Note that a suspension of blank xPRO‐SBA (containing no dye) at the respective concentration was used to correct for scattered light in the absorption spectrum.

Techniques Used: Fluorescence, Concentration Assay

a) Fluorescence emission spectra ( λ ex =490 nm) of xPRO‐SBA‐ I (0.11 mg mL −1 in Milli‐Q water, pH 7) in the presence of different amounts of Hg II . Inset: Corresponding normalized absorption spectra. b) Corresponding fluorescence enhancement ratio (Δ F / F 0 ) registered at λ =538 nm ( λ ex =490 nm) as a function of Hg II added in Milli‐Q Water (black line, pH 7), acetate buffer (red line; 10 m m , pH 4), and phosphate buffer (blue line; 10 m m . pH 7). Inset: Magnification of the low‐concentration range.
Figure Legend Snippet: a) Fluorescence emission spectra ( λ ex =490 nm) of xPRO‐SBA‐ I (0.11 mg mL −1 in Milli‐Q water, pH 7) in the presence of different amounts of Hg II . Inset: Corresponding normalized absorption spectra. b) Corresponding fluorescence enhancement ratio (Δ F / F 0 ) registered at λ =538 nm ( λ ex =490 nm) as a function of Hg II added in Milli‐Q Water (black line, pH 7), acetate buffer (red line; 10 m m , pH 4), and phosphate buffer (blue line; 10 m m . pH 7). Inset: Magnification of the low‐concentration range.

Techniques Used: Fluorescence, Concentration Assay

Absorption spectra of BODIPY dye I (1.1 μ m ; black lines) in a) water and c) MeCN in the absence and in the presence of 1 ppm of cations Hg II (green line), Ag I (red line), Ni II (blue line), and Cu II (orange line; the bleaching behavior of Cu II , which only occurs in MeCN yet not in H 2 O or aq. MeCN, was observed before, see Ref. 41 ). Corresponding fluorescence emission spectra ( λ ex =490 nm) of I (1.1 μ m ) in b) Milli‐Q water at pH 7 and d) MeCN. Inset bottom: Corresponding photographs under UV light ( λ ex =365 nm) of an initial solution of BODIPY I dye (1.1 μ m ) in the presence of (from left to right) 1 ppm of Hg II , Ag I , Ni II , and Cu II .
Figure Legend Snippet: Absorption spectra of BODIPY dye I (1.1 μ m ; black lines) in a) water and c) MeCN in the absence and in the presence of 1 ppm of cations Hg II (green line), Ag I (red line), Ni II (blue line), and Cu II (orange line; the bleaching behavior of Cu II , which only occurs in MeCN yet not in H 2 O or aq. MeCN, was observed before, see Ref. 41 ). Corresponding fluorescence emission spectra ( λ ex =490 nm) of I (1.1 μ m ) in b) Milli‐Q water at pH 7 and d) MeCN. Inset bottom: Corresponding photographs under UV light ( λ ex =365 nm) of an initial solution of BODIPY I dye (1.1 μ m ) in the presence of (from left to right) 1 ppm of Hg II , Ag I , Ni II , and Cu II .

Techniques Used: Fluorescence

5) Product Images from "Evaluation of the stability of linezolid in aqueous solution and commonly used intravenous fluids"

Article Title: Evaluation of the stability of linezolid in aqueous solution and commonly used intravenous fluids

Journal: Drug Design, Development and Therapy

doi: 10.2147/DDDT.S136335

Forced degradation of linezolid ([i] initial concentration and [ii] concentration after 24 hours) in 0.1 M sodium hydroxide solution ( A ), 0.1 M hydrochloric acid ( B ), 0.6% hydrogen peroxide ( C ), or Milli-Q water ( D ).
Figure Legend Snippet: Forced degradation of linezolid ([i] initial concentration and [ii] concentration after 24 hours) in 0.1 M sodium hydroxide solution ( A ), 0.1 M hydrochloric acid ( B ), 0.6% hydrogen peroxide ( C ), or Milli-Q water ( D ).

Techniques Used: Concentration Assay

6) Product Images from "Mix‐ ‐Read Determination of Mercury(II) at Trace Levels with Hybrid Mesoporous Silica Materials Incorporating Fluorescent Probes by a Simple Mix‐ ‐Load Technique"

Article Title: Mix‐ ‐Read Determination of Mercury(II) at Trace Levels with Hybrid Mesoporous Silica Materials Incorporating Fluorescent Probes by a Simple Mix‐ ‐Load Technique

Journal: ChemistryOpen

doi: 10.1002/open.201800111

a) Absorption spectrum (red) and fluorescence excitation (solid black; λ em =564 nm) and emission (dashed black; λ ex =490 nm) spectra of xPRO‐SBA‐ I′ in Milli‐Q water (0.11 mg mL −1 ; pH 7). b) Normalized absorption spectra of a suspension of xPRO‐SBA‐ I′ in the presence of various amounts of Hg II , increasing from red to blue. c) Fluorescence titration spectra ( λ ex =490 nm) of xPRO‐SBA‐ I′ suspended in Milli‐Q water (0.11 mg mL −1 ; pH 7) upon the addition of Hg II . Inset: Corresponding fluorescence excitation spectra ( λ em =564 nm). d) Corresponding fluorescence enhancement ratio (Δ F / F 0 ) registered at λ =538 nm ( λ ex =490 nm) for xPRO‐SBA‐ I′ suspended in Milli‐Q water (0.11 mg mL −1 ; pH 7) in the presence of increasing amounts of Hg II . Inset: Magnification of the low‐concentration range.
Figure Legend Snippet: a) Absorption spectrum (red) and fluorescence excitation (solid black; λ em =564 nm) and emission (dashed black; λ ex =490 nm) spectra of xPRO‐SBA‐ I′ in Milli‐Q water (0.11 mg mL −1 ; pH 7). b) Normalized absorption spectra of a suspension of xPRO‐SBA‐ I′ in the presence of various amounts of Hg II , increasing from red to blue. c) Fluorescence titration spectra ( λ ex =490 nm) of xPRO‐SBA‐ I′ suspended in Milli‐Q water (0.11 mg mL −1 ; pH 7) upon the addition of Hg II . Inset: Corresponding fluorescence excitation spectra ( λ em =564 nm). d) Corresponding fluorescence enhancement ratio (Δ F / F 0 ) registered at λ =538 nm ( λ ex =490 nm) for xPRO‐SBA‐ I′ suspended in Milli‐Q water (0.11 mg mL −1 ; pH 7) in the presence of increasing amounts of Hg II . Inset: Magnification of the low‐concentration range.

Techniques Used: Fluorescence, Titration, Concentration Assay

a) Absorption (solid red line) and fluorescence excitation ( λ em =564 nm) and emission spectra ( λ ex =490 nm; solid black lines) of xPRO‐SBA‐ I in Milli‐Q water (0.11 mg mL −1 , pH 7). b) Fluorescence intensity registered at λ =538 nm ( λ ex =490 nm) of xPRO‐SBA‐ I in Milli‐Q water (0.11 mg mL −1 , pH 7) as a function of time in the absence (blue line) and presence (black line) of Hg II (500 ppb). Note that a suspension of blank xPRO‐SBA (containing no dye) at the respective concentration was used to correct for scattered light in the absorption spectrum.
Figure Legend Snippet: a) Absorption (solid red line) and fluorescence excitation ( λ em =564 nm) and emission spectra ( λ ex =490 nm; solid black lines) of xPRO‐SBA‐ I in Milli‐Q water (0.11 mg mL −1 , pH 7). b) Fluorescence intensity registered at λ =538 nm ( λ ex =490 nm) of xPRO‐SBA‐ I in Milli‐Q water (0.11 mg mL −1 , pH 7) as a function of time in the absence (blue line) and presence (black line) of Hg II (500 ppb). Note that a suspension of blank xPRO‐SBA (containing no dye) at the respective concentration was used to correct for scattered light in the absorption spectrum.

Techniques Used: Fluorescence, Concentration Assay

7) Product Images from "Mapping of Enzyme Kinetics on a Microfluidic Device"

Article Title: Mapping of Enzyme Kinetics on a Microfluidic Device

Journal: PLoS ONE

doi: 10.1371/journal.pone.0153437

Generation of concentration gradient of fluorescein. (A) Design of the compositions of solutions in 36 reactors. Each reactor consists of four loading sites for enzyme (orange), substrate I (yellow), substrate II (blue), and buffer (green). (B) Fluorescence intensities of 36 reactors with concentration gradients of fluorescein as a negative control. The gradient of concentration of fluorescein was obtained by loading fluorescein solution into only one loading site while Milli-Q water was introduced into the other three sites. The obtained fluorescence intensities measured in the 36 reactors match with the target compositions (error bars represent the standard deviation from three measurements).
Figure Legend Snippet: Generation of concentration gradient of fluorescein. (A) Design of the compositions of solutions in 36 reactors. Each reactor consists of four loading sites for enzyme (orange), substrate I (yellow), substrate II (blue), and buffer (green). (B) Fluorescence intensities of 36 reactors with concentration gradients of fluorescein as a negative control. The gradient of concentration of fluorescein was obtained by loading fluorescein solution into only one loading site while Milli-Q water was introduced into the other three sites. The obtained fluorescence intensities measured in the 36 reactors match with the target compositions (error bars represent the standard deviation from three measurements).

Techniques Used: Concentration Assay, Fluorescence, Negative Control, Standard Deviation

8) Product Images from "Characterization of protein immobilization on nanoporous gold using atomic force microscopy and scanning electron microscopy †"

Article Title: Characterization of protein immobilization on nanoporous gold using atomic force microscopy and scanning electron microscopy †

Journal: Nanoscale

doi: 10.1039/c1nr10427f

Tapping mode-AFM topographs of the EDC functionalized rough gold surface after adsorption of bovine serum albumin. (A) 2000 nm × 2000 nm, EDC treated rough gold surface without exposure to BSA. Similarly, 2000 nm × 2000 nm, AFM topographic images of EDC functionalized rough gold surface after exposure to 1.0 μg ml −1 BSA, for (B) 1 min, (C) 10 min, and (D) 15 min. (E–F) Higher resolution, AFM topographic images, 500 nm × 500 nm, of the areas indicated by the boxes in (A–D), respectively. The sample was thoroughly rinsed with 1% Tween 20, and Milli-Q water, before images were acquired using AFM.
Figure Legend Snippet: Tapping mode-AFM topographs of the EDC functionalized rough gold surface after adsorption of bovine serum albumin. (A) 2000 nm × 2000 nm, EDC treated rough gold surface without exposure to BSA. Similarly, 2000 nm × 2000 nm, AFM topographic images of EDC functionalized rough gold surface after exposure to 1.0 μg ml −1 BSA, for (B) 1 min, (C) 10 min, and (D) 15 min. (E–F) Higher resolution, AFM topographic images, 500 nm × 500 nm, of the areas indicated by the boxes in (A–D), respectively. The sample was thoroughly rinsed with 1% Tween 20, and Milli-Q water, before images were acquired using AFM.

Techniques Used: Adsorption

Tapping mode-AFM topographs of the NHS functionalized rough gold surface after adsorption of bovine serum albumin. (A) 2000 nm × 2000 nm, NHS treated rough gold surface without exposure to BSA. Similarly, 2000 nm × 2000 nm, AFM topographic images of NHS active esters rough gold surface after exposure to 1.0 μg ml −1 BSA, for (B) 1 min, (C) 10 min, and (D) 15 min. (E–H) Higher resolution, AFM topographic images, 500 nm × 500 nm, of the areas indicated by the boxes in (A–D), respectively. The sample was thoroughly rinsed with 1% Tween 20, and Milli-Q water, before images were acquired using AFM.
Figure Legend Snippet: Tapping mode-AFM topographs of the NHS functionalized rough gold surface after adsorption of bovine serum albumin. (A) 2000 nm × 2000 nm, NHS treated rough gold surface without exposure to BSA. Similarly, 2000 nm × 2000 nm, AFM topographic images of NHS active esters rough gold surface after exposure to 1.0 μg ml −1 BSA, for (B) 1 min, (C) 10 min, and (D) 15 min. (E–H) Higher resolution, AFM topographic images, 500 nm × 500 nm, of the areas indicated by the boxes in (A–D), respectively. The sample was thoroughly rinsed with 1% Tween 20, and Milli-Q water, before images were acquired using AFM.

Techniques Used: Adsorption

9) Product Images from "Spectrophotometric Online Detection of Drinking Water Disinfectant: A Machine Learning Approach"

Article Title: Spectrophotometric Online Detection of Drinking Water Disinfectant: A Machine Learning Approach

Journal: Sensors (Basel, Switzerland)

doi: 10.3390/s20226671

( a ) Monochloramine spectra in Milli-Q water with different levels of concentration. ( b ) First derivative of spectra. ( c ) Uncompensated spectra, and ( d ) particle compensated spectra.
Figure Legend Snippet: ( a ) Monochloramine spectra in Milli-Q water with different levels of concentration. ( b ) First derivative of spectra. ( c ) Uncompensated spectra, and ( d ) particle compensated spectra.

Techniques Used: Concentration Assay

10) Product Images from "Rational design of sequestered DNAzyme beacons to enable flexible control of catalytic activities †"

Article Title: Rational design of sequestered DNAzyme beacons to enable flexible control of catalytic activities †

Journal: RSC Advances

doi: 10.1039/c8ra05757e

Sensing system for the detection of arbitrary target sequence. (A) Schematic representation of the general target sensing strategy. A metastable dual stem-loop structure probe was designed by combining recognition and DNAzyme modules. (B) Time-dependent fluorescence response on varying concentrations of target. The curves show the change in fluorescence in the presence of different concentrations of the target (nM): 0, 0.5, 1, 2, 5, 10, 15, 20, 50, and 100 from bottom to top, respectively. (C) The linear responses of fluorescence enhancement at low target concentrations. (D) Fluorescence enhancement of the sensing system for a series of targets (15 nM): target sequence, single-base mutant sequences, control sample, and target sequence spiked or unspiked in 5-fold diluted HeLa cell lysates, respectively. The error bars represent the standard deviation of three measurements.
Figure Legend Snippet: Sensing system for the detection of arbitrary target sequence. (A) Schematic representation of the general target sensing strategy. A metastable dual stem-loop structure probe was designed by combining recognition and DNAzyme modules. (B) Time-dependent fluorescence response on varying concentrations of target. The curves show the change in fluorescence in the presence of different concentrations of the target (nM): 0, 0.5, 1, 2, 5, 10, 15, 20, 50, and 100 from bottom to top, respectively. (C) The linear responses of fluorescence enhancement at low target concentrations. (D) Fluorescence enhancement of the sensing system for a series of targets (15 nM): target sequence, single-base mutant sequences, control sample, and target sequence spiked or unspiked in 5-fold diluted HeLa cell lysates, respectively. The error bars represent the standard deviation of three measurements.

Techniques Used: Sequencing, Fluorescence, Mutagenesis, Standard Deviation

Mechanism proposed for the sequestered DNAzyme beacons (SDBs) with controllable catalytic activities. Inset shows the secondary structure of Zn( ii )-dependent deoxyribozyme–substrate complex 17E/DS. SDB is constructed by 17E (blue domain) with an extended blocking sequence (red domain) attached at the 3′ end. The catalytic core of 17E is controllably sequestered in the stem of the hairpin structure. The hairpin structure of SDB can be reversibly unfolded. The 17E in SDB can be then liberated and hybridize with a fluorophore and quencher labelled substrate to form a catalytic system. The nucleotide sequences of all strands are provided in Table S1 (ESI † ).
Figure Legend Snippet: Mechanism proposed for the sequestered DNAzyme beacons (SDBs) with controllable catalytic activities. Inset shows the secondary structure of Zn( ii )-dependent deoxyribozyme–substrate complex 17E/DS. SDB is constructed by 17E (blue domain) with an extended blocking sequence (red domain) attached at the 3′ end. The catalytic core of 17E is controllably sequestered in the stem of the hairpin structure. The hairpin structure of SDB can be reversibly unfolded. The 17E in SDB can be then liberated and hybridize with a fluorophore and quencher labelled substrate to form a catalytic system. The nucleotide sequences of all strands are provided in Table S1 (ESI † ).

Techniques Used: Construct, Blocking Assay, Sequencing

11) Product Images from "Tumor-specific hyperthermia with aptamer-tagged superparamagnetic nanoparticles"

Article Title: Tumor-specific hyperthermia with aptamer-tagged superparamagnetic nanoparticles

Journal: International Journal of Nanomedicine

doi: 10.2147/IJN.S52539

Dynamic light scattering of dextran-coated magnetic nanoparticles. Notes: Dynamic light scattering measured in H 2 O Milli-Q (EMD Millipore, Billerica, MA, USA) at 25°C.
Figure Legend Snippet: Dynamic light scattering of dextran-coated magnetic nanoparticles. Notes: Dynamic light scattering measured in H 2 O Milli-Q (EMD Millipore, Billerica, MA, USA) at 25°C.

Techniques Used:

12) Product Images from "Anisotropic Polymer Adsorption on Molybdenite Basal and Edge Surfaces and Interaction Mechanism With Air Bubbles"

Article Title: Anisotropic Polymer Adsorption on Molybdenite Basal and Edge Surfaces and Interaction Mechanism With Air Bubbles

Journal: Frontiers in Chemistry

doi: 10.3389/fchem.2018.00361

AFM height ( Left ) and phase ( Right ) images of MoS 2 basal plane conditioned at different CMC concentrations in Milli-Q water at pH 9 for 30 min: (A) 0 ppm (5 × 5 μm 2 ), (B) 5 ppm (5 × 5 μm 2 ), (C) 10 ppm (5 × 5 μm 2 ), (D) 100 ppm (5 × 5 μm 2 ), and (E) 100 ppm (2 × 2 μm 2 ).
Figure Legend Snippet: AFM height ( Left ) and phase ( Right ) images of MoS 2 basal plane conditioned at different CMC concentrations in Milli-Q water at pH 9 for 30 min: (A) 0 ppm (5 × 5 μm 2 ), (B) 5 ppm (5 × 5 μm 2 ), (C) 10 ppm (5 × 5 μm 2 ), (D) 100 ppm (5 × 5 μm 2 ), and (E) 100 ppm (2 × 2 μm 2 ).

Techniques Used:

AFM height images (5 × 5 μm 2 ) of MoS 2 edge plane conditioned in 100 ppm CMC solution in Milli-Q water at pH 9 for different adsorption times: (A) 0 min, (B) 30 min, (C) 60 min, (D) 90 min, (E) 120 min, and (F) 180 min.
Figure Legend Snippet: AFM height images (5 × 5 μm 2 ) of MoS 2 edge plane conditioned in 100 ppm CMC solution in Milli-Q water at pH 9 for different adsorption times: (A) 0 min, (B) 30 min, (C) 60 min, (D) 90 min, (E) 120 min, and (F) 180 min.

Techniques Used: Adsorption

13) Product Images from "Tumor-specific hyperthermia with aptamer-tagged superparamagnetic nanoparticles"

Article Title: Tumor-specific hyperthermia with aptamer-tagged superparamagnetic nanoparticles

Journal: International Journal of Nanomedicine

doi: 10.2147/IJN.S52539

Dynamic light scattering of dextran-coated magnetic nanoparticles. Notes: Dynamic light scattering measured in H 2 O Milli-Q (EMD Millipore, Billerica, MA, USA) at 25°C.
Figure Legend Snippet: Dynamic light scattering of dextran-coated magnetic nanoparticles. Notes: Dynamic light scattering measured in H 2 O Milli-Q (EMD Millipore, Billerica, MA, USA) at 25°C.

Techniques Used:

14) Product Images from "A Novel Tool for Visualization of Water Molecular Structure and Its Changes, Expressed on the Scale of Temperature Influence"

Article Title: A Novel Tool for Visualization of Water Molecular Structure and Its Changes, Expressed on the Scale of Temperature Influence

Journal: Molecules

doi: 10.3390/molecules25092234

Aquagrams with 95% confidence intervals of Milli-Q water ( n = 150) and 0.001–0.01 M KCl solutions ( n = 60) calculated with the temperature based aquagram calculation method on the individual concentrations (UCL—upper confidence level, LCL—lower confidence level).
Figure Legend Snippet: Aquagrams with 95% confidence intervals of Milli-Q water ( n = 150) and 0.001–0.01 M KCl solutions ( n = 60) calculated with the temperature based aquagram calculation method on the individual concentrations (UCL—upper confidence level, LCL—lower confidence level).

Techniques Used:

Raw and 2nd derivative (calculated with Savitzky–Golay filter using 2nd order polynomial and 21 points) absorbance (logT −1 ) spectra in the spectral range of 1300–1600 nm (OH first overtone) of Milli-Q water in the temperature range of 20–70 °C ( n = 78).
Figure Legend Snippet: Raw and 2nd derivative (calculated with Savitzky–Golay filter using 2nd order polynomial and 21 points) absorbance (logT −1 ) spectra in the spectral range of 1300–1600 nm (OH first overtone) of Milli-Q water in the temperature range of 20–70 °C ( n = 78).

Techniques Used:

Aquagrams with 95% confidence intervals of Milli-Q water ( n = 150) and 0.001–1 M KCl solutions ( n = 180) calculated with the classic ( a ) or the temperature based aquagram ( b ) calculation methods on the individual concentrations (UCL—upper confidence level, LCL—lower confidence level).
Figure Legend Snippet: Aquagrams with 95% confidence intervals of Milli-Q water ( n = 150) and 0.001–1 M KCl solutions ( n = 180) calculated with the classic ( a ) or the temperature based aquagram ( b ) calculation methods on the individual concentrations (UCL—upper confidence level, LCL—lower confidence level).

Techniques Used:

Aquagrams of Milli-Q water in the temperature range of 20–70 °C, ( a , b ) with 95% confidence intervals calculated with temperature-based aquagram calculation method, ( c , d ) calculated with the classic calculation method, ( a , c ) all the 26 temperature steps ( n = 78) and ( b , d ) on three selected temperature steps ( n = 9) to show the stability of the methods (UCL—upper confidence level, LCL—lower confidence level).
Figure Legend Snippet: Aquagrams of Milli-Q water in the temperature range of 20–70 °C, ( a , b ) with 95% confidence intervals calculated with temperature-based aquagram calculation method, ( c , d ) calculated with the classic calculation method, ( a , c ) all the 26 temperature steps ( n = 78) and ( b , d ) on three selected temperature steps ( n = 9) to show the stability of the methods (UCL—upper confidence level, LCL—lower confidence level).

Techniques Used:

Aquagrams with 95% confidence intervals of Milli-Q water ( n = 150) and 0.001–0.1 M KCl solutions ( n = 120) calculated with the temperature based aquagram calculation method on the individual concentrations (UCL—upper confidence level, LCL—lower confidence level).
Figure Legend Snippet: Aquagrams with 95% confidence intervals of Milli-Q water ( n = 150) and 0.001–0.1 M KCl solutions ( n = 120) calculated with the temperature based aquagram calculation method on the individual concentrations (UCL—upper confidence level, LCL—lower confidence level).

Techniques Used:

15) Product Images from "A Novel Tool for Visualization of Water Molecular Structure and Its Changes, Expressed on the Scale of Temperature Influence"

Article Title: A Novel Tool for Visualization of Water Molecular Structure and Its Changes, Expressed on the Scale of Temperature Influence

Journal: Molecules

doi: 10.3390/molecules25092234

Aquagrams with 95% confidence intervals of Milli-Q water ( n = 150) and 0.001–0.01 M KCl solutions ( n = 60) calculated with the temperature based aquagram calculation method on the individual concentrations (UCL—upper confidence level, LCL—lower confidence level).
Figure Legend Snippet: Aquagrams with 95% confidence intervals of Milli-Q water ( n = 150) and 0.001–0.01 M KCl solutions ( n = 60) calculated with the temperature based aquagram calculation method on the individual concentrations (UCL—upper confidence level, LCL—lower confidence level).

Techniques Used:

Raw and 2nd derivative (calculated with Savitzky–Golay filter using 2nd order polynomial and 21 points) absorbance (logT −1 ) spectra in the spectral range of 1300–1600 nm (OH first overtone) of Milli-Q water in the temperature range of 20–70 °C ( n = 78).
Figure Legend Snippet: Raw and 2nd derivative (calculated with Savitzky–Golay filter using 2nd order polynomial and 21 points) absorbance (logT −1 ) spectra in the spectral range of 1300–1600 nm (OH first overtone) of Milli-Q water in the temperature range of 20–70 °C ( n = 78).

Techniques Used:

Aquagrams with 95% confidence intervals of Milli-Q water ( n = 150) and 0.001–1 M KCl solutions ( n = 180) calculated with the classic ( a ) or the temperature based aquagram ( b ) calculation methods on the individual concentrations (UCL—upper confidence level, LCL—lower confidence level).
Figure Legend Snippet: Aquagrams with 95% confidence intervals of Milli-Q water ( n = 150) and 0.001–1 M KCl solutions ( n = 180) calculated with the classic ( a ) or the temperature based aquagram ( b ) calculation methods on the individual concentrations (UCL—upper confidence level, LCL—lower confidence level).

Techniques Used:

Aquagrams of Milli-Q water in the temperature range of 20–70 °C, ( a , b ) with 95% confidence intervals calculated with temperature-based aquagram calculation method, ( c , d ) calculated with the classic calculation method, ( a , c ) all the 26 temperature steps ( n = 78) and ( b , d ) on three selected temperature steps ( n = 9) to show the stability of the methods (UCL—upper confidence level, LCL—lower confidence level).
Figure Legend Snippet: Aquagrams of Milli-Q water in the temperature range of 20–70 °C, ( a , b ) with 95% confidence intervals calculated with temperature-based aquagram calculation method, ( c , d ) calculated with the classic calculation method, ( a , c ) all the 26 temperature steps ( n = 78) and ( b , d ) on three selected temperature steps ( n = 9) to show the stability of the methods (UCL—upper confidence level, LCL—lower confidence level).

Techniques Used:

Aquagrams with 95% confidence intervals of Milli-Q water ( n = 150) and 0.001–0.1 M KCl solutions ( n = 120) calculated with the temperature based aquagram calculation method on the individual concentrations (UCL—upper confidence level, LCL—lower confidence level).
Figure Legend Snippet: Aquagrams with 95% confidence intervals of Milli-Q water ( n = 150) and 0.001–0.1 M KCl solutions ( n = 120) calculated with the temperature based aquagram calculation method on the individual concentrations (UCL—upper confidence level, LCL—lower confidence level).

Techniques Used:

16) Product Images from "Mix‐ ‐Read Determination of Mercury(II) at Trace Levels with Hybrid Mesoporous Silica Materials Incorporating Fluorescent Probes by a Simple Mix‐ ‐Load Technique"

Article Title: Mix‐ ‐Read Determination of Mercury(II) at Trace Levels with Hybrid Mesoporous Silica Materials Incorporating Fluorescent Probes by a Simple Mix‐ ‐Load Technique

Journal: ChemistryOpen

doi: 10.1002/open.201800111

a) Intensity of fluorescence registered at λ =538 nm ( λ ex =490 nm) of xPRO‐SBA‐ I suspended in Milli‐Q water (0.11 mg mL −1 ; pH 7) in the presence of 6 μ m of Mn II , Co II , Zn II , Cd II , Mg II , Fe II , Cu II , Ni II , Hg II , and Ag I . b) Fluorescence enhancement in the corresponding suspensions as a function of the concentration of Ag I , Hg II , Ni II , and Cu II .
Figure Legend Snippet: a) Intensity of fluorescence registered at λ =538 nm ( λ ex =490 nm) of xPRO‐SBA‐ I suspended in Milli‐Q water (0.11 mg mL −1 ; pH 7) in the presence of 6 μ m of Mn II , Co II , Zn II , Cd II , Mg II , Fe II , Cu II , Ni II , Hg II , and Ag I . b) Fluorescence enhancement in the corresponding suspensions as a function of the concentration of Ag I , Hg II , Ni II , and Cu II .

Techniques Used: Fluorescence, Concentration Assay

a) Fluorescence intensity at λ =540 nm of I in water (1.7 μ m in H 2 O at pH 7; λ ex =490 nm) registered as a function of time while stirring the solution in a cuvette in the absence (blue line) and presence (black line) of Hg II (500 ppb). Red lines correspond to fits of the data to a first‐order kinetic reaction model. b) Absorption spectra of I (1.7 μ m , Milli‐Q water, pH 7) in the absence and in the presence of Hg II (0–3 ppm). Inset: Photograph showing the color changing from pink to yellow; see also Figure S1 for a larger version. From left to right, c Hg =0, 0.1, and 0.8 ppm. c) Corresponding fluorescence spectra ( λ ex =490 nm). d) Normalized excitation (d.1, top; λ em =564 nm) and emission (d.2, bottom; λ ex =490 nm) titration spectra. e) Plot of fluorescence enhancement ratio, Δ F / F 0 , that is, ( F − F 0 )/ F 0 , registered at λ =538 nm ( λ ex =490 nm) as a function of Hg II added. Inset: Photograph showing change in fluorescence under UV light ( λ ex =365 nm) in the absence and in the presence of Hg II (2 ppm).
Figure Legend Snippet: a) Fluorescence intensity at λ =540 nm of I in water (1.7 μ m in H 2 O at pH 7; λ ex =490 nm) registered as a function of time while stirring the solution in a cuvette in the absence (blue line) and presence (black line) of Hg II (500 ppb). Red lines correspond to fits of the data to a first‐order kinetic reaction model. b) Absorption spectra of I (1.7 μ m , Milli‐Q water, pH 7) in the absence and in the presence of Hg II (0–3 ppm). Inset: Photograph showing the color changing from pink to yellow; see also Figure S1 for a larger version. From left to right, c Hg =0, 0.1, and 0.8 ppm. c) Corresponding fluorescence spectra ( λ ex =490 nm). d) Normalized excitation (d.1, top; λ em =564 nm) and emission (d.2, bottom; λ ex =490 nm) titration spectra. e) Plot of fluorescence enhancement ratio, Δ F / F 0 , that is, ( F − F 0 )/ F 0 , registered at λ =538 nm ( λ ex =490 nm) as a function of Hg II added. Inset: Photograph showing change in fluorescence under UV light ( λ ex =365 nm) in the absence and in the presence of Hg II (2 ppm).

Techniques Used: Fluorescence, Titration

a) Absorption spectrum (red) and fluorescence excitation (solid black; λ em =564 nm) and emission (dashed black; λ ex =490 nm) spectra of xPRO‐SBA‐ I′ in Milli‐Q water (0.11 mg mL −1 ; pH 7). b) Normalized absorption spectra of a suspension of xPRO‐SBA‐ I′ in the presence of various amounts of Hg II , increasing from red to blue. c) Fluorescence titration spectra ( λ ex =490 nm) of xPRO‐SBA‐ I′ suspended in Milli‐Q water (0.11 mg mL −1 ; pH 7) upon the addition of Hg II . Inset: Corresponding fluorescence excitation spectra ( λ em =564 nm). d) Corresponding fluorescence enhancement ratio (Δ F / F 0 ) registered at λ =538 nm ( λ ex =490 nm) for xPRO‐SBA‐ I′ suspended in Milli‐Q water (0.11 mg mL −1 ; pH 7) in the presence of increasing amounts of Hg II . Inset: Magnification of the low‐concentration range.
Figure Legend Snippet: a) Absorption spectrum (red) and fluorescence excitation (solid black; λ em =564 nm) and emission (dashed black; λ ex =490 nm) spectra of xPRO‐SBA‐ I′ in Milli‐Q water (0.11 mg mL −1 ; pH 7). b) Normalized absorption spectra of a suspension of xPRO‐SBA‐ I′ in the presence of various amounts of Hg II , increasing from red to blue. c) Fluorescence titration spectra ( λ ex =490 nm) of xPRO‐SBA‐ I′ suspended in Milli‐Q water (0.11 mg mL −1 ; pH 7) upon the addition of Hg II . Inset: Corresponding fluorescence excitation spectra ( λ em =564 nm). d) Corresponding fluorescence enhancement ratio (Δ F / F 0 ) registered at λ =538 nm ( λ ex =490 nm) for xPRO‐SBA‐ I′ suspended in Milli‐Q water (0.11 mg mL −1 ; pH 7) in the presence of increasing amounts of Hg II . Inset: Magnification of the low‐concentration range.

Techniques Used: Fluorescence, Titration, Concentration Assay

a) Absorption spectra (solid lines) and fluorescence excitation spectra ( λ em =564 nm; dashed lines) of BODIPY dye I ( c= 1.6 μ m ) in MeCN (black) and in Milli‐Q water at pH 7 (red). b) Corresponding fluorescence emission spectra ( λ ex =490 nm) of I in MeCN (black) and Milli‐Q water at pH 7 (red).
Figure Legend Snippet: a) Absorption spectra (solid lines) and fluorescence excitation spectra ( λ em =564 nm; dashed lines) of BODIPY dye I ( c= 1.6 μ m ) in MeCN (black) and in Milli‐Q water at pH 7 (red). b) Corresponding fluorescence emission spectra ( λ ex =490 nm) of I in MeCN (black) and Milli‐Q water at pH 7 (red).

Techniques Used: Fluorescence

a) Absorption (solid red line) and fluorescence excitation ( λ em =564 nm) and emission spectra ( λ ex =490 nm; solid black lines) of xPRO‐SBA‐ I in Milli‐Q water (0.11 mg mL −1 , pH 7). b) Fluorescence intensity registered at λ =538 nm ( λ ex =490 nm) of xPRO‐SBA‐ I in Milli‐Q water (0.11 mg mL −1 , pH 7) as a function of time in the absence (blue line) and presence (black line) of Hg II (500 ppb). Note that a suspension of blank xPRO‐SBA (containing no dye) at the respective concentration was used to correct for scattered light in the absorption spectrum.
Figure Legend Snippet: a) Absorption (solid red line) and fluorescence excitation ( λ em =564 nm) and emission spectra ( λ ex =490 nm; solid black lines) of xPRO‐SBA‐ I in Milli‐Q water (0.11 mg mL −1 , pH 7). b) Fluorescence intensity registered at λ =538 nm ( λ ex =490 nm) of xPRO‐SBA‐ I in Milli‐Q water (0.11 mg mL −1 , pH 7) as a function of time in the absence (blue line) and presence (black line) of Hg II (500 ppb). Note that a suspension of blank xPRO‐SBA (containing no dye) at the respective concentration was used to correct for scattered light in the absorption spectrum.

Techniques Used: Fluorescence, Concentration Assay

a) Fluorescence emission spectra ( λ ex =490 nm) of xPRO‐SBA‐ I (0.11 mg mL −1 in Milli‐Q water, pH 7) in the presence of different amounts of Hg II . Inset: Corresponding normalized absorption spectra. b) Corresponding fluorescence enhancement ratio (Δ F / F 0 ) registered at λ =538 nm ( λ ex =490 nm) as a function of Hg II added in Milli‐Q Water (black line, pH 7), acetate buffer (red line; 10 m m , pH 4), and phosphate buffer (blue line; 10 m m . pH 7). Inset: Magnification of the low‐concentration range.
Figure Legend Snippet: a) Fluorescence emission spectra ( λ ex =490 nm) of xPRO‐SBA‐ I (0.11 mg mL −1 in Milli‐Q water, pH 7) in the presence of different amounts of Hg II . Inset: Corresponding normalized absorption spectra. b) Corresponding fluorescence enhancement ratio (Δ F / F 0 ) registered at λ =538 nm ( λ ex =490 nm) as a function of Hg II added in Milli‐Q Water (black line, pH 7), acetate buffer (red line; 10 m m , pH 4), and phosphate buffer (blue line; 10 m m . pH 7). Inset: Magnification of the low‐concentration range.

Techniques Used: Fluorescence, Concentration Assay

Absorption spectra of BODIPY dye I (1.1 μ m ; black lines) in a) water and c) MeCN in the absence and in the presence of 1 ppm of cations Hg II (green line), Ag I (red line), Ni II (blue line), and Cu II (orange line; the bleaching behavior of Cu II , which only occurs in MeCN yet not in H 2 O or aq. MeCN, was observed before, see Ref. 41 ). Corresponding fluorescence emission spectra ( λ ex =490 nm) of I (1.1 μ m ) in b) Milli‐Q water at pH 7 and d) MeCN. Inset bottom: Corresponding photographs under UV light ( λ ex =365 nm) of an initial solution of BODIPY I dye (1.1 μ m ) in the presence of (from left to right) 1 ppm of Hg II , Ag I , Ni II , and Cu II .
Figure Legend Snippet: Absorption spectra of BODIPY dye I (1.1 μ m ; black lines) in a) water and c) MeCN in the absence and in the presence of 1 ppm of cations Hg II (green line), Ag I (red line), Ni II (blue line), and Cu II (orange line; the bleaching behavior of Cu II , which only occurs in MeCN yet not in H 2 O or aq. MeCN, was observed before, see Ref. 41 ). Corresponding fluorescence emission spectra ( λ ex =490 nm) of I (1.1 μ m ) in b) Milli‐Q water at pH 7 and d) MeCN. Inset bottom: Corresponding photographs under UV light ( λ ex =365 nm) of an initial solution of BODIPY I dye (1.1 μ m ) in the presence of (from left to right) 1 ppm of Hg II , Ag I , Ni II , and Cu II .

Techniques Used: Fluorescence

17) Product Images from "Detection of UV-induced cyclobutane pyrimidine dimers by near-infrared spectroscopy and aquaphotomics"

Article Title: Detection of UV-induced cyclobutane pyrimidine dimers by near-infrared spectroscopy and aquaphotomics

Journal: Scientific Reports

doi: 10.1038/srep11808

NIRS-based discrimination of Milli-Q water and cis-syn T
Figure Legend Snippet: NIRS-based discrimination of Milli-Q water and cis-syn T

Techniques Used:

18) Product Images from "Essentials of Aquaphotomics and Its Chemometrics Approaches"

Article Title: Essentials of Aquaphotomics and Its Chemometrics Approaches

Journal: Frontiers in Chemistry

doi: 10.3389/fchem.2018.00363

Aquagrams without confidence intervals of Milli-Q water and aqueous solutions of potassium-chloride in the concentration range of 10–100 mM calculated on the MSC transformed absorbance (logT-1) spectra in the spectral range of 1,300–1,600 nm (OH first overtone) using the “temperature-based” mode.
Figure Legend Snippet: Aquagrams without confidence intervals of Milli-Q water and aqueous solutions of potassium-chloride in the concentration range of 10–100 mM calculated on the MSC transformed absorbance (logT-1) spectra in the spectral range of 1,300–1,600 nm (OH first overtone) using the “temperature-based” mode.

Techniques Used: Concentration Assay, Transformation Assay

Aquagrams with 95% confidence intervals of Milli-Q water and aqueous solutions of potassium-chloride in the concentration range of 10–100 mM calculated on the MSC transformed absorbance (logT-1) spectra in the spectral range of 1,300–1,600 nm (OH first overtone) using the linearized version of the “temperature-based” mode with average values.
Figure Legend Snippet: Aquagrams with 95% confidence intervals of Milli-Q water and aqueous solutions of potassium-chloride in the concentration range of 10–100 mM calculated on the MSC transformed absorbance (logT-1) spectra in the spectral range of 1,300–1,600 nm (OH first overtone) using the linearized version of the “temperature-based” mode with average values.

Techniques Used: Concentration Assay, Transformation Assay

Smoothed (calculated with a Savitzky-Golay filter using 21 points) absorbance (logT-1) spectra in the spectral range of 1,300–1,600 nm (OH first overtone) of Milli-Q water and aqueous solutions of potassium-chloride in the concentration range of 10–100 mM.
Figure Legend Snippet: Smoothed (calculated with a Savitzky-Golay filter using 21 points) absorbance (logT-1) spectra in the spectral range of 1,300–1,600 nm (OH first overtone) of Milli-Q water and aqueous solutions of potassium-chloride in the concentration range of 10–100 mM.

Techniques Used: Concentration Assay

PCA analysis of Milli-Q water and aqueous solutions of potassium-chloride in the concentration range of 10–100 mM derived from the smoothed (calculated with a Savitzky-Golay filter using 2nd order polynomial and 21 points) and MSC transformed absorbance (logT-1) spectra in the spectral range of 1,300–1,600 nm (OH first overtone)—Loadings plot.
Figure Legend Snippet: PCA analysis of Milli-Q water and aqueous solutions of potassium-chloride in the concentration range of 10–100 mM derived from the smoothed (calculated with a Savitzky-Golay filter using 2nd order polynomial and 21 points) and MSC transformed absorbance (logT-1) spectra in the spectral range of 1,300–1,600 nm (OH first overtone)—Loadings plot.

Techniques Used: Concentration Assay, Derivative Assay, Transformation Assay

Raw absorbance (logT-1) spectra in the entire spectral range of Milli-Q water and aqueous solutions of potassium-chloride in the concentration range of 10–100 mM.
Figure Legend Snippet: Raw absorbance (logT-1) spectra in the entire spectral range of Milli-Q water and aqueous solutions of potassium-chloride in the concentration range of 10–100 mM.

Techniques Used: Concentration Assay

Aquagrams without confidence intervals of Milli-Q water and aqueous solutions of potassium-chloride in the concentration range of 10–100 mM calculated on the MSC transformed absorbance (logT-1) spectra in the spectral range of 1,300–1,600 nm (OH first overtone) using the “classic” mode.
Figure Legend Snippet: Aquagrams without confidence intervals of Milli-Q water and aqueous solutions of potassium-chloride in the concentration range of 10–100 mM calculated on the MSC transformed absorbance (logT-1) spectra in the spectral range of 1,300–1,600 nm (OH first overtone) using the “classic” mode.

Techniques Used: Concentration Assay, Transformation Assay

PCA analysis of Milli-Q water and aqueous solutions of potassium-chloride in the concentration range of 10–100 mM derived from the smoothed (calculated with a Savitzky-Golay filter using 2nd order polynomial and 21 points) and MSC transformed absorbance (logT-1) spectra in the spectral range of 1,300–1,600 nm (OH first overtone)—Scores plots for the first six principal components.
Figure Legend Snippet: PCA analysis of Milli-Q water and aqueous solutions of potassium-chloride in the concentration range of 10–100 mM derived from the smoothed (calculated with a Savitzky-Golay filter using 2nd order polynomial and 21 points) and MSC transformed absorbance (logT-1) spectra in the spectral range of 1,300–1,600 nm (OH first overtone)—Scores plots for the first six principal components.

Techniques Used: Concentration Assay, Derivative Assay, Transformation Assay

PLSR analysis of Milli-Q water and aqueous solutions of potassium-chloride in the concentration range of 10–100 mM derived from the smoothed (calculated with a Savitzky-Golay filter using 2nd order polynomial and 21 points) and MSC transformed absorbance (logT-1) spectra in the spectral range of 1,300–1,600 nm (OH first overtone) built for the prediction of potassium-chloride concentration: Y fit of training and one-sample-out cross-validation.
Figure Legend Snippet: PLSR analysis of Milli-Q water and aqueous solutions of potassium-chloride in the concentration range of 10–100 mM derived from the smoothed (calculated with a Savitzky-Golay filter using 2nd order polynomial and 21 points) and MSC transformed absorbance (logT-1) spectra in the spectral range of 1,300–1,600 nm (OH first overtone) built for the prediction of potassium-chloride concentration: Y fit of training and one-sample-out cross-validation.

Techniques Used: Concentration Assay, Derivative Assay, Transformation Assay

Aquagrams with 95% confidence intervals of Milli-Q water and aqueous solutions of potassium-chloride in the concentration range of 10–100 mM calculated on the MSC transformed absorbance (logT-1) spectra in the spectral range of 1,300–1,600 nm (OH first overtone) using the “classic” mode.
Figure Legend Snippet: Aquagrams with 95% confidence intervals of Milli-Q water and aqueous solutions of potassium-chloride in the concentration range of 10–100 mM calculated on the MSC transformed absorbance (logT-1) spectra in the spectral range of 1,300–1,600 nm (OH first overtone) using the “classic” mode.

Techniques Used: Concentration Assay, Transformation Assay

2nd derivative (calculated with a Savitzky-Golay filter using 2nd order polynomial and 21 points) average absorbance (logT-1) spectra in the spectral range of 1,300–1,600 nm (OH first overtone) of Milli-Q water and aqueous solutions of potassium-chloride in the concentration range of 10–100 mM.
Figure Legend Snippet: 2nd derivative (calculated with a Savitzky-Golay filter using 2nd order polynomial and 21 points) average absorbance (logT-1) spectra in the spectral range of 1,300–1,600 nm (OH first overtone) of Milli-Q water and aqueous solutions of potassium-chloride in the concentration range of 10–100 mM.

Techniques Used: Concentration Assay

Smoothed (calculated with a Savitzky-Golay filter using 21 points) average difference absorbance (logT-1) spectra in the spectral range of 1,300–1,600 nm (OH first overtone) of Milli-Q water and aqueous solutions of potassium-chloride in the concentration range of 10–100 mM. Average spectrum of Milli-Q water was subtracted from the spectra of potassium-chloride solutions.
Figure Legend Snippet: Smoothed (calculated with a Savitzky-Golay filter using 21 points) average difference absorbance (logT-1) spectra in the spectral range of 1,300–1,600 nm (OH first overtone) of Milli-Q water and aqueous solutions of potassium-chloride in the concentration range of 10–100 mM. Average spectrum of Milli-Q water was subtracted from the spectra of potassium-chloride solutions.

Techniques Used: Concentration Assay

PCA analysis of Milli-Q water and aqueous solutions of potassium-chloride in the concentration range of 10–100 mM derived from the smoothed (calculated with a Savitzky-Golay filter using 2nd order polynomial and 21 points) and MSC transformed absorbance (logT-1) spectra in the spectral range of 1,300–1,600 nm (OH first overtone)—Scores plots for the first two principal components.
Figure Legend Snippet: PCA analysis of Milli-Q water and aqueous solutions of potassium-chloride in the concentration range of 10–100 mM derived from the smoothed (calculated with a Savitzky-Golay filter using 2nd order polynomial and 21 points) and MSC transformed absorbance (logT-1) spectra in the spectral range of 1,300–1,600 nm (OH first overtone)—Scores plots for the first two principal components.

Techniques Used: Concentration Assay, Derivative Assay, Transformation Assay

Aquagrams with 95% confidence intervals of Milli-Q water and aqueous solutions of potassium-chloride in the concentration range of 10–100 mM calculated on the MSC transformed absorbance (logT-1) spectra in the spectral range of 1,300–1,600 nm (OH first overtone) using the “temperature-based” mode.
Figure Legend Snippet: Aquagrams with 95% confidence intervals of Milli-Q water and aqueous solutions of potassium-chloride in the concentration range of 10–100 mM calculated on the MSC transformed absorbance (logT-1) spectra in the spectral range of 1,300–1,600 nm (OH first overtone) using the “temperature-based” mode.

Techniques Used: Concentration Assay, Transformation Assay

PLSR analysis of Milli-Q water and aqueous solutions of potassium-chloride in the concentration range of 10–100 mM derived from the smoothed (calculated with a Savitzky-Golay filter using 2nd order polynomial and 21 points) and MSC transformed absorbance (logT-1) spectra in the spectral range of 1,300–1,600 nm (OH first overtone) built for the prediction of potassium-chloride concentration: Regression vector.
Figure Legend Snippet: PLSR analysis of Milli-Q water and aqueous solutions of potassium-chloride in the concentration range of 10–100 mM derived from the smoothed (calculated with a Savitzky-Golay filter using 2nd order polynomial and 21 points) and MSC transformed absorbance (logT-1) spectra in the spectral range of 1,300–1,600 nm (OH first overtone) built for the prediction of potassium-chloride concentration: Regression vector.

Techniques Used: Concentration Assay, Derivative Assay, Transformation Assay, Plasmid Preparation

19) Product Images from "Effect of papain-based gel on type I collagen - spectroscopy applied for microstructural analysis"

Article Title: Effect of papain-based gel on type I collagen - spectroscopy applied for microstructural analysis

Journal: Scientific Reports

doi: 10.1038/srep11448

Mean infrared type I collagen spectrum obtained for dry membranes and membranes washed with Milli-Q water (hydrated) in region from 3800 to 800 cm −1 .
Figure Legend Snippet: Mean infrared type I collagen spectrum obtained for dry membranes and membranes washed with Milli-Q water (hydrated) in region from 3800 to 800 cm −1 .

Techniques Used:

20) Product Images from "Mix‐ ‐Read Determination of Mercury(II) at Trace Levels with Hybrid Mesoporous Silica Materials Incorporating Fluorescent Probes by a Simple Mix‐ ‐Load Technique"

Article Title: Mix‐ ‐Read Determination of Mercury(II) at Trace Levels with Hybrid Mesoporous Silica Materials Incorporating Fluorescent Probes by a Simple Mix‐ ‐Load Technique

Journal: ChemistryOpen

doi: 10.1002/open.201800277

a) Absorption spectrum (red) and fluorescence excitation (solid black; λ em =564 nm) and emission (dashed black; λ ex =490 nm) spectra of xPRO‐SBA‐ I′ in Milli‐Q water (0.11 mg mL −1 ; pH 7). b) Normalized absorption spectra of a suspension of xPRO‐SBA‐ I′ in the presence of various amounts of Hg II , increasing from red to blue. c) Fluorescence titration spectra ( λ ex =490 nm) of xPRO‐SBA‐ I′ suspended in Milli‐Q water (0.11 mg mL −1 ; pH 7) upon the addition of Hg II . Inset: Corresponding fluorescence excitation spectra ( λ em =564 nm). d) Corresponding fluorescence enhancement ratio (Δ F / F 0 ) registered at λ =538 nm ( λ ex =490 nm) for xPRO‐SBA‐ I′ suspended in Milli‐Q water (0.11 mg mL −1 ; pH 7) in the presence of increasing amounts of Hg II . Inset: Magnification of the low‐concentration range.
Figure Legend Snippet: a) Absorption spectrum (red) and fluorescence excitation (solid black; λ em =564 nm) and emission (dashed black; λ ex =490 nm) spectra of xPRO‐SBA‐ I′ in Milli‐Q water (0.11 mg mL −1 ; pH 7). b) Normalized absorption spectra of a suspension of xPRO‐SBA‐ I′ in the presence of various amounts of Hg II , increasing from red to blue. c) Fluorescence titration spectra ( λ ex =490 nm) of xPRO‐SBA‐ I′ suspended in Milli‐Q water (0.11 mg mL −1 ; pH 7) upon the addition of Hg II . Inset: Corresponding fluorescence excitation spectra ( λ em =564 nm). d) Corresponding fluorescence enhancement ratio (Δ F / F 0 ) registered at λ =538 nm ( λ ex =490 nm) for xPRO‐SBA‐ I′ suspended in Milli‐Q water (0.11 mg mL −1 ; pH 7) in the presence of increasing amounts of Hg II . Inset: Magnification of the low‐concentration range.

Techniques Used: Fluorescence, Titration, Concentration Assay

a) Absorption (solid red line) and fluorescence excitation ( λ em =564 nm) and emission spectra ( λ ex =490 nm; solid black lines) of xPRO‐SBA‐ I in Milli‐Q water (0.11 mg mL −1 , pH 7). b) Fluorescence intensity registered at λ =538 nm ( λ ex =490 nm) of xPRO‐SBA‐ I in Milli‐Q water (0.11 mg mL −1 , pH 7) as a function of time in the absence (blue line) and presence (black line) of Hg II (500 ppb). Note that a suspension of blank xPRO‐SBA (containing no dye) at the respective concentration was used to correct for scattered light in the absorption spectrum.
Figure Legend Snippet: a) Absorption (solid red line) and fluorescence excitation ( λ em =564 nm) and emission spectra ( λ ex =490 nm; solid black lines) of xPRO‐SBA‐ I in Milli‐Q water (0.11 mg mL −1 , pH 7). b) Fluorescence intensity registered at λ =538 nm ( λ ex =490 nm) of xPRO‐SBA‐ I in Milli‐Q water (0.11 mg mL −1 , pH 7) as a function of time in the absence (blue line) and presence (black line) of Hg II (500 ppb). Note that a suspension of blank xPRO‐SBA (containing no dye) at the respective concentration was used to correct for scattered light in the absorption spectrum.

Techniques Used: Fluorescence, Concentration Assay

21) Product Images from "Mapping of Enzyme Kinetics on a Microfluidic Device"

Article Title: Mapping of Enzyme Kinetics on a Microfluidic Device

Journal: PLoS ONE

doi: 10.1371/journal.pone.0153437

Generation of concentration gradient of fluorescein. (A) Design of the compositions of solutions in 36 reactors. Each reactor consists of four loading sites for enzyme (orange), substrate I (yellow), substrate II (blue), and buffer (green). (B) Fluorescence intensities of 36 reactors with concentration gradients of fluorescein as a negative control. The gradient of concentration of fluorescein was obtained by loading fluorescein solution into only one loading site while Milli-Q water was introduced into the other three sites. The obtained fluorescence intensities measured in the 36 reactors match with the target compositions (error bars represent the standard deviation from three measurements).
Figure Legend Snippet: Generation of concentration gradient of fluorescein. (A) Design of the compositions of solutions in 36 reactors. Each reactor consists of four loading sites for enzyme (orange), substrate I (yellow), substrate II (blue), and buffer (green). (B) Fluorescence intensities of 36 reactors with concentration gradients of fluorescein as a negative control. The gradient of concentration of fluorescein was obtained by loading fluorescein solution into only one loading site while Milli-Q water was introduced into the other three sites. The obtained fluorescence intensities measured in the 36 reactors match with the target compositions (error bars represent the standard deviation from three measurements).

Techniques Used: Concentration Assay, Fluorescence, Negative Control, Standard Deviation

22) Product Images from "Effect of papain-based gel on type I collagen - spectroscopy applied for microstructural analysis"

Article Title: Effect of papain-based gel on type I collagen - spectroscopy applied for microstructural analysis

Journal: Scientific Reports

doi: 10.1038/srep11448

Mean infrared type I collagen spectrum obtained for dry membranes and membranes washed with Milli-Q water (hydrated) in region from 3800 to 800 cm −1 .
Figure Legend Snippet: Mean infrared type I collagen spectrum obtained for dry membranes and membranes washed with Milli-Q water (hydrated) in region from 3800 to 800 cm −1 .

Techniques Used:

23) Product Images from "Anisotropic Polymer Adsorption on Molybdenite Basal and Edge Surfaces and Interaction Mechanism With Air Bubbles"

Article Title: Anisotropic Polymer Adsorption on Molybdenite Basal and Edge Surfaces and Interaction Mechanism With Air Bubbles

Journal: Frontiers in Chemistry

doi: 10.3389/fchem.2018.00361

AFM height ( Left ) and phase ( Right ) images of MoS 2 basal plane conditioned at different CMC concentrations in Milli-Q water at pH 9 for 30 min: (A) 0 ppm (5 × 5 μm 2 ), (B) 5 ppm (5 × 5 μm 2 ), (C) 10 ppm (5 × 5 μm 2 ), (D) 100 ppm (5 × 5 μm 2 ), and (E) 100 ppm (2 × 2 μm 2 ).
Figure Legend Snippet: AFM height ( Left ) and phase ( Right ) images of MoS 2 basal plane conditioned at different CMC concentrations in Milli-Q water at pH 9 for 30 min: (A) 0 ppm (5 × 5 μm 2 ), (B) 5 ppm (5 × 5 μm 2 ), (C) 10 ppm (5 × 5 μm 2 ), (D) 100 ppm (5 × 5 μm 2 ), and (E) 100 ppm (2 × 2 μm 2 ).

Techniques Used:

AFM height images (5 × 5 μm 2 ) of MoS 2 edge plane conditioned in 100 ppm CMC solution in Milli-Q water at pH 9 for different adsorption times: (A) 0 min, (B) 30 min, (C) 60 min, (D) 90 min, (E) 120 min, and (F) 180 min.
Figure Legend Snippet: AFM height images (5 × 5 μm 2 ) of MoS 2 edge plane conditioned in 100 ppm CMC solution in Milli-Q water at pH 9 for different adsorption times: (A) 0 min, (B) 30 min, (C) 60 min, (D) 90 min, (E) 120 min, and (F) 180 min.

Techniques Used: Adsorption

24) Product Images from "Purification and identification of antioxidative peptides from mackerel (Pneumatophorus japonicus) protein"

Article Title: Purification and identification of antioxidative peptides from mackerel (Pneumatophorus japonicus) protein

Journal: RSC Advances

doi: 10.1039/c8ra03350a

Response surface plots showing the effects of variables on the cellular antioxidant activity of MPH. X -axis and Y -axis: A (enzyme concentration); B (extraction time); C (pH); D (water/material ratio); E (extraction temperature). Z -axis: cellular antioxidant activity (%).
Figure Legend Snippet: Response surface plots showing the effects of variables on the cellular antioxidant activity of MPH. X -axis and Y -axis: A (enzyme concentration); B (extraction time); C (pH); D (water/material ratio); E (extraction temperature). Z -axis: cellular antioxidant activity (%).

Techniques Used: Antioxidant Activity Assay, Concentration Assay

25) Product Images from "Kinetic and mechanistic investigations of the degradation of propranolol in heat activated persulfate process †"

Article Title: Kinetic and mechanistic investigations of the degradation of propranolol in heat activated persulfate process †

Journal: RSC Advances

doi: 10.1039/c8ra08488b

The removal of PRO by heat/PS in real water matrix. Conditions: [PRO] 0 = 0.025 mM, [PS] 0 = 1 mM, T = 60 °C.
Figure Legend Snippet: The removal of PRO by heat/PS in real water matrix. Conditions: [PRO] 0 = 0.025 mM, [PS] 0 = 1 mM, T = 60 °C.

Techniques Used:

(a) EPR spectra obtained from DMPO experiments after 5 min. (b) Identification of predominant radical species in heat/PS system. Conditions: [PRO] 0 = 0.025 mM, [PS] 0 = 1 mM, pH = 7, T = 60 °C, [DMPO] 0 = 0.1 M, [Scavenger] 0 /[PS] 0 = 400 : 1.
Figure Legend Snippet: (a) EPR spectra obtained from DMPO experiments after 5 min. (b) Identification of predominant radical species in heat/PS system. Conditions: [PRO] 0 = 0.025 mM, [PS] 0 = 1 mM, pH = 7, T = 60 °C, [DMPO] 0 = 0.1 M, [Scavenger] 0 /[PS] 0 = 400 : 1.

Techniques Used:

26) Product Images from "Anisotropic Polymer Adsorption on Molybdenite Basal and Edge Surfaces and Interaction Mechanism With Air Bubbles"

Article Title: Anisotropic Polymer Adsorption on Molybdenite Basal and Edge Surfaces and Interaction Mechanism With Air Bubbles

Journal: Frontiers in Chemistry

doi: 10.3389/fchem.2018.00361

AFM height ( Left ) and phase ( Right ) images of MoS 2 basal plane conditioned at different CMC concentrations in Milli-Q water at pH 9 for 30 min: (A) 0 ppm (5 × 5 μm 2 ), (B) 5 ppm (5 × 5 μm 2 ), (C) 10 ppm (5 × 5 μm 2 ), (D) 100 ppm (5 × 5 μm 2 ), and (E) 100 ppm (2 × 2 μm 2 ).
Figure Legend Snippet: AFM height ( Left ) and phase ( Right ) images of MoS 2 basal plane conditioned at different CMC concentrations in Milli-Q water at pH 9 for 30 min: (A) 0 ppm (5 × 5 μm 2 ), (B) 5 ppm (5 × 5 μm 2 ), (C) 10 ppm (5 × 5 μm 2 ), (D) 100 ppm (5 × 5 μm 2 ), and (E) 100 ppm (2 × 2 μm 2 ).

Techniques Used:

AFM height images (5 × 5 μm 2 ) of MoS 2 edge plane conditioned in 100 ppm CMC solution in Milli-Q water at pH 9 for different adsorption times: (A) 0 min, (B) 30 min, (C) 60 min, (D) 90 min, (E) 120 min, and (F) 180 min.
Figure Legend Snippet: AFM height images (5 × 5 μm 2 ) of MoS 2 edge plane conditioned in 100 ppm CMC solution in Milli-Q water at pH 9 for different adsorption times: (A) 0 min, (B) 30 min, (C) 60 min, (D) 90 min, (E) 120 min, and (F) 180 min.

Techniques Used: Adsorption

27) Product Images from "Methacrylic-based nanogels for the pH-sensitive delivery of 5-Fluorouracil in the colon"

Article Title: Methacrylic-based nanogels for the pH-sensitive delivery of 5-Fluorouracil in the colon

Journal: International Journal of Nanomedicine

doi: 10.2147/IJN.S31201

AFM image of MAEHA nanogels. ( A and C ) Phase images; ( B and D ) amplitude images. Notes: For AFM, the sample of 1 μL of MAEHA nanogel suspension was dropped onto freshly cleaved mica surface and washed with 50 μL of Milli-Q ® water. Measurements were taken only after the sample had completely dried. Abbreviations: AFM, atomic force microscopy; MAEHA, methacrylic acid-co-2-ethyl hexyl acrylate.
Figure Legend Snippet: AFM image of MAEHA nanogels. ( A and C ) Phase images; ( B and D ) amplitude images. Notes: For AFM, the sample of 1 μL of MAEHA nanogel suspension was dropped onto freshly cleaved mica surface and washed with 50 μL of Milli-Q ® water. Measurements were taken only after the sample had completely dried. Abbreviations: AFM, atomic force microscopy; MAEHA, methacrylic acid-co-2-ethyl hexyl acrylate.

Techniques Used: Microscopy

TEM image of MAEHA nanogels. Notes: For TEM, the sample of MAEHA nanogel suspension in Milli-Q ® water was dropped onto formvar-coated copper grids without being negatively stained. Measurements were taken only after the sample had completely dried. Abbreviations: TEM, transmission electron microscopy; MAEHA, methacrylic acid-co-2-ethyl hexyl acrylate.
Figure Legend Snippet: TEM image of MAEHA nanogels. Notes: For TEM, the sample of MAEHA nanogel suspension in Milli-Q ® water was dropped onto formvar-coated copper grids without being negatively stained. Measurements were taken only after the sample had completely dried. Abbreviations: TEM, transmission electron microscopy; MAEHA, methacrylic acid-co-2-ethyl hexyl acrylate.

Techniques Used: Transmission Electron Microscopy, Staining, Transmission Assay, Electron Microscopy

28) Product Images from "Determination of Sulfonamides in Feeds by High-Performance Liquid Chromatography after Fluorescamine Precolumn Derivatization"

Article Title: Determination of Sulfonamides in Feeds by High-Performance Liquid Chromatography after Fluorescamine Precolumn Derivatization

Journal: Molecules

doi: 10.3390/molecules24030452

Chromatogram of feed sample spiked with five SAs at a concentration of 200 µg/kg on a Zorbax Eclipse XDB C18 column with a mobile phase of 0.1% acetic acid in Milli-Q water/acetonitrile/methanol.
Figure Legend Snippet: Chromatogram of feed sample spiked with five SAs at a concentration of 200 µg/kg on a Zorbax Eclipse XDB C18 column with a mobile phase of 0.1% acetic acid in Milli-Q water/acetonitrile/methanol.

Techniques Used: Concentration Assay

Chromatogram of feed sample spiked with five SAs at a concentration of 200 µg/kg on a Zorbax Eclipse XDB C18 column with a mobile phase of 0.1% formic acid in Milli-Q water/acetonitrile/methanol.
Figure Legend Snippet: Chromatogram of feed sample spiked with five SAs at a concentration of 200 µg/kg on a Zorbax Eclipse XDB C18 column with a mobile phase of 0.1% formic acid in Milli-Q water/acetonitrile/methanol.

Techniques Used: Concentration Assay

Chromatogram of feed sample spiked with five SAs at a concentration of 200 µg/kg on a Zorbax Eclipse XDB C18 column with a mobile phase of 0.08% acetic acid in Milli-Q water/acetonitrilemethanol.
Figure Legend Snippet: Chromatogram of feed sample spiked with five SAs at a concentration of 200 µg/kg on a Zorbax Eclipse XDB C18 column with a mobile phase of 0.08% acetic acid in Milli-Q water/acetonitrilemethanol.

Techniques Used: Concentration Assay

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    Millipore milli q water
    NIRS-based discrimination of <t>Milli-Q</t> water and cis-syn T
    Milli Q Water, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 67 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    NIRS-based discrimination of Milli-Q water and cis-syn T

    Journal: Scientific Reports

    Article Title: Detection of UV-induced cyclobutane pyrimidine dimers by near-infrared spectroscopy and aquaphotomics

    doi: 10.1038/srep11808

    Figure Lengend Snippet: NIRS-based discrimination of Milli-Q water and cis-syn T

    Article Snippet: After UVC exposure, 1 mL Milli-Q water was added to the experimental DNA solutions in order to reach the volume required for NIRS measurements.

    Techniques:

    ( a ) The UV-Vis absorption of PPy-IC, PPy-KC, PPy-IC/MWNT and PPy-KC/MWNT composite solutions at a concentration of 1.0 mg/mL in Milli-Q water; ( b ) Stability of PPy-IC, PPy-KC, PPy-IC/MWNT and PPy-KC/MWNT composite solutions at a concentration of 2.0 mg/mL in Milli-Q water.

    Journal: Polymers

    Article Title: Chemical and Electrochemical Synthesis of Polypyrrole Using Carrageenan as a Dopant: Polypyrrole/Multi-Walled Carbon Nanotube Nanocomposites

    doi: 10.3390/polym10060632

    Figure Lengend Snippet: ( a ) The UV-Vis absorption of PPy-IC, PPy-KC, PPy-IC/MWNT and PPy-KC/MWNT composite solutions at a concentration of 1.0 mg/mL in Milli-Q water; ( b ) Stability of PPy-IC, PPy-KC, PPy-IC/MWNT and PPy-KC/MWNT composite solutions at a concentration of 2.0 mg/mL in Milli-Q water.

    Article Snippet: Milli-Q water (resistivity =18.2 MΩ·cm) from a Millipore Q water purification system was used in all experiments.

    Techniques: Concentration Assay

    ( a ) UV-Vis absorption spectra of PPy-IC at different concentrations in Milli-Q water and ( b ) the absorbance at 970 nm of different concentrations of PPy-IC.

    Journal: Polymers

    Article Title: Chemical and Electrochemical Synthesis of Polypyrrole Using Carrageenan as a Dopant: Polypyrrole/Multi-Walled Carbon Nanotube Nanocomposites

    doi: 10.3390/polym10060632

    Figure Lengend Snippet: ( a ) UV-Vis absorption spectra of PPy-IC at different concentrations in Milli-Q water and ( b ) the absorbance at 970 nm of different concentrations of PPy-IC.

    Article Snippet: Milli-Q water (resistivity =18.2 MΩ·cm) from a Millipore Q water purification system was used in all experiments.

    Techniques:

    OJIP transients recorded from the youngest, fully expanded barley leaves. A, Main plot. Transients were recorded just before P resupply at 21 DAP. The inset shows transients recorded 7 d after P resupply at 28 DAP. The slope of the quenching curve was calculated between the two dashed vertical lines (between 2 and 10 s). B, Transients recorded for P-deficient leaves immersed in Milli-Q water (P deficient) or P solution (P resupply) for 60 min. All transients were averaged (A, n = 5; B, n = 4, each with more than four technical replicates) and doubled normalized between F 0 and F m . A.U., Arbitrary units.

    Journal: Plant Physiology

    Article Title: The Impacts of Phosphorus Deficiency on the Photosynthetic Electron Transport Chain 1The Impacts of Phosphorus Deficiency on the Photosynthetic Electron Transport Chain 1 [OPEN]

    doi: 10.1104/pp.17.01624

    Figure Lengend Snippet: OJIP transients recorded from the youngest, fully expanded barley leaves. A, Main plot. Transients were recorded just before P resupply at 21 DAP. The inset shows transients recorded 7 d after P resupply at 28 DAP. The slope of the quenching curve was calculated between the two dashed vertical lines (between 2 and 10 s). B, Transients recorded for P-deficient leaves immersed in Milli-Q water (P deficient) or P solution (P resupply) for 60 min. All transients were averaged (A, n = 5; B, n = 4, each with more than four technical replicates) and doubled normalized between F 0 and F m . A.U., Arbitrary units.

    Article Snippet: Then, 1 mL of HS buffer was added, and the suspension was layered onto a precooled Percoll pad containing 2 mL of 5× HS buffer, 3.5 mL of Percoll, and 4.5 mL of Milli-Q water and centrifuged at 1,400 g for 8 min in a swing rotor.

    Techniques:

    a) Intensity of fluorescence registered at λ =538 nm ( λ ex =490 nm) of xPRO‐SBA‐ I suspended in Milli‐Q water (0.11 mg mL −1 ; pH 7) in the presence of 6 μ m of Mn II , Co II , Zn II , Cd II , Mg II , Fe II , Cu II , Ni II , Hg II , and Ag I . b) Fluorescence enhancement in the corresponding suspensions as a function of the concentration of Ag I , Hg II , Ni II , and Cu II .

    Journal: ChemistryOpen

    Article Title: Mix‐ ‐Read Determination of Mercury(II) at Trace Levels with Hybrid Mesoporous Silica Materials Incorporating Fluorescent Probes by a Simple Mix‐ ‐Load Technique

    doi: 10.1002/open.201800111

    Figure Lengend Snippet: a) Intensity of fluorescence registered at λ =538 nm ( λ ex =490 nm) of xPRO‐SBA‐ I suspended in Milli‐Q water (0.11 mg mL −1 ; pH 7) in the presence of 6 μ m of Mn II , Co II , Zn II , Cd II , Mg II , Fe II , Cu II , Ni II , Hg II , and Ag I . b) Fluorescence enhancement in the corresponding suspensions as a function of the concentration of Ag I , Hg II , Ni II , and Cu II .

    Article Snippet: Phosphate buffer and acetate buffer solutions (10 mm ) were prepared with ultrapure reagent water, which was obtained by running demineralized water (by ion exchange) through a Milli‐Q ultrapure water purification system (Millipore Synthesis A10).

    Techniques: Fluorescence, Concentration Assay

    a) Fluorescence intensity at λ =540 nm of I in water (1.7 μ m in H 2 O at pH 7; λ ex =490 nm) registered as a function of time while stirring the solution in a cuvette in the absence (blue line) and presence (black line) of Hg II (500 ppb). Red lines correspond to fits of the data to a first‐order kinetic reaction model. b) Absorption spectra of I (1.7 μ m , Milli‐Q water, pH 7) in the absence and in the presence of Hg II (0–3 ppm). Inset: Photograph showing the color changing from pink to yellow; see also Figure S1 for a larger version. From left to right, c Hg =0, 0.1, and 0.8 ppm. c) Corresponding fluorescence spectra ( λ ex =490 nm). d) Normalized excitation (d.1, top; λ em =564 nm) and emission (d.2, bottom; λ ex =490 nm) titration spectra. e) Plot of fluorescence enhancement ratio, Δ F / F 0 , that is, ( F − F 0 )/ F 0 , registered at λ =538 nm ( λ ex =490 nm) as a function of Hg II added. Inset: Photograph showing change in fluorescence under UV light ( λ ex =365 nm) in the absence and in the presence of Hg II (2 ppm).

    Journal: ChemistryOpen

    Article Title: Mix‐ ‐Read Determination of Mercury(II) at Trace Levels with Hybrid Mesoporous Silica Materials Incorporating Fluorescent Probes by a Simple Mix‐ ‐Load Technique

    doi: 10.1002/open.201800111

    Figure Lengend Snippet: a) Fluorescence intensity at λ =540 nm of I in water (1.7 μ m in H 2 O at pH 7; λ ex =490 nm) registered as a function of time while stirring the solution in a cuvette in the absence (blue line) and presence (black line) of Hg II (500 ppb). Red lines correspond to fits of the data to a first‐order kinetic reaction model. b) Absorption spectra of I (1.7 μ m , Milli‐Q water, pH 7) in the absence and in the presence of Hg II (0–3 ppm). Inset: Photograph showing the color changing from pink to yellow; see also Figure S1 for a larger version. From left to right, c Hg =0, 0.1, and 0.8 ppm. c) Corresponding fluorescence spectra ( λ ex =490 nm). d) Normalized excitation (d.1, top; λ em =564 nm) and emission (d.2, bottom; λ ex =490 nm) titration spectra. e) Plot of fluorescence enhancement ratio, Δ F / F 0 , that is, ( F − F 0 )/ F 0 , registered at λ =538 nm ( λ ex =490 nm) as a function of Hg II added. Inset: Photograph showing change in fluorescence under UV light ( λ ex =365 nm) in the absence and in the presence of Hg II (2 ppm).

    Article Snippet: Phosphate buffer and acetate buffer solutions (10 mm ) were prepared with ultrapure reagent water, which was obtained by running demineralized water (by ion exchange) through a Milli‐Q ultrapure water purification system (Millipore Synthesis A10).

    Techniques: Fluorescence, Titration

    a) Absorption spectrum (red) and fluorescence excitation (solid black; λ em =564 nm) and emission (dashed black; λ ex =490 nm) spectra of xPRO‐SBA‐ I′ in Milli‐Q water (0.11 mg mL −1 ; pH 7). b) Normalized absorption spectra of a suspension of xPRO‐SBA‐ I′ in the presence of various amounts of Hg II , increasing from red to blue. c) Fluorescence titration spectra ( λ ex =490 nm) of xPRO‐SBA‐ I′ suspended in Milli‐Q water (0.11 mg mL −1 ; pH 7) upon the addition of Hg II . Inset: Corresponding fluorescence excitation spectra ( λ em =564 nm). d) Corresponding fluorescence enhancement ratio (Δ F / F 0 ) registered at λ =538 nm ( λ ex =490 nm) for xPRO‐SBA‐ I′ suspended in Milli‐Q water (0.11 mg mL −1 ; pH 7) in the presence of increasing amounts of Hg II . Inset: Magnification of the low‐concentration range.

    Journal: ChemistryOpen

    Article Title: Mix‐ ‐Read Determination of Mercury(II) at Trace Levels with Hybrid Mesoporous Silica Materials Incorporating Fluorescent Probes by a Simple Mix‐ ‐Load Technique

    doi: 10.1002/open.201800111

    Figure Lengend Snippet: a) Absorption spectrum (red) and fluorescence excitation (solid black; λ em =564 nm) and emission (dashed black; λ ex =490 nm) spectra of xPRO‐SBA‐ I′ in Milli‐Q water (0.11 mg mL −1 ; pH 7). b) Normalized absorption spectra of a suspension of xPRO‐SBA‐ I′ in the presence of various amounts of Hg II , increasing from red to blue. c) Fluorescence titration spectra ( λ ex =490 nm) of xPRO‐SBA‐ I′ suspended in Milli‐Q water (0.11 mg mL −1 ; pH 7) upon the addition of Hg II . Inset: Corresponding fluorescence excitation spectra ( λ em =564 nm). d) Corresponding fluorescence enhancement ratio (Δ F / F 0 ) registered at λ =538 nm ( λ ex =490 nm) for xPRO‐SBA‐ I′ suspended in Milli‐Q water (0.11 mg mL −1 ; pH 7) in the presence of increasing amounts of Hg II . Inset: Magnification of the low‐concentration range.

    Article Snippet: Phosphate buffer and acetate buffer solutions (10 mm ) were prepared with ultrapure reagent water, which was obtained by running demineralized water (by ion exchange) through a Milli‐Q ultrapure water purification system (Millipore Synthesis A10).

    Techniques: Fluorescence, Titration, Concentration Assay

    a) Absorption spectra (solid lines) and fluorescence excitation spectra ( λ em =564 nm; dashed lines) of BODIPY dye I ( c= 1.6 μ m ) in MeCN (black) and in Milli‐Q water at pH 7 (red). b) Corresponding fluorescence emission spectra ( λ ex =490 nm) of I in MeCN (black) and Milli‐Q water at pH 7 (red).

    Journal: ChemistryOpen

    Article Title: Mix‐ ‐Read Determination of Mercury(II) at Trace Levels with Hybrid Mesoporous Silica Materials Incorporating Fluorescent Probes by a Simple Mix‐ ‐Load Technique

    doi: 10.1002/open.201800111

    Figure Lengend Snippet: a) Absorption spectra (solid lines) and fluorescence excitation spectra ( λ em =564 nm; dashed lines) of BODIPY dye I ( c= 1.6 μ m ) in MeCN (black) and in Milli‐Q water at pH 7 (red). b) Corresponding fluorescence emission spectra ( λ ex =490 nm) of I in MeCN (black) and Milli‐Q water at pH 7 (red).

    Article Snippet: Phosphate buffer and acetate buffer solutions (10 mm ) were prepared with ultrapure reagent water, which was obtained by running demineralized water (by ion exchange) through a Milli‐Q ultrapure water purification system (Millipore Synthesis A10).

    Techniques: Fluorescence

    a) Absorption (solid red line) and fluorescence excitation ( λ em =564 nm) and emission spectra ( λ ex =490 nm; solid black lines) of xPRO‐SBA‐ I in Milli‐Q water (0.11 mg mL −1 , pH 7). b) Fluorescence intensity registered at λ =538 nm ( λ ex =490 nm) of xPRO‐SBA‐ I in Milli‐Q water (0.11 mg mL −1 , pH 7) as a function of time in the absence (blue line) and presence (black line) of Hg II (500 ppb). Note that a suspension of blank xPRO‐SBA (containing no dye) at the respective concentration was used to correct for scattered light in the absorption spectrum.

    Journal: ChemistryOpen

    Article Title: Mix‐ ‐Read Determination of Mercury(II) at Trace Levels with Hybrid Mesoporous Silica Materials Incorporating Fluorescent Probes by a Simple Mix‐ ‐Load Technique

    doi: 10.1002/open.201800111

    Figure Lengend Snippet: a) Absorption (solid red line) and fluorescence excitation ( λ em =564 nm) and emission spectra ( λ ex =490 nm; solid black lines) of xPRO‐SBA‐ I in Milli‐Q water (0.11 mg mL −1 , pH 7). b) Fluorescence intensity registered at λ =538 nm ( λ ex =490 nm) of xPRO‐SBA‐ I in Milli‐Q water (0.11 mg mL −1 , pH 7) as a function of time in the absence (blue line) and presence (black line) of Hg II (500 ppb). Note that a suspension of blank xPRO‐SBA (containing no dye) at the respective concentration was used to correct for scattered light in the absorption spectrum.

    Article Snippet: Phosphate buffer and acetate buffer solutions (10 mm ) were prepared with ultrapure reagent water, which was obtained by running demineralized water (by ion exchange) through a Milli‐Q ultrapure water purification system (Millipore Synthesis A10).

    Techniques: Fluorescence, Concentration Assay

    a) Fluorescence emission spectra ( λ ex =490 nm) of xPRO‐SBA‐ I (0.11 mg mL −1 in Milli‐Q water, pH 7) in the presence of different amounts of Hg II . Inset: Corresponding normalized absorption spectra. b) Corresponding fluorescence enhancement ratio (Δ F / F 0 ) registered at λ =538 nm ( λ ex =490 nm) as a function of Hg II added in Milli‐Q Water (black line, pH 7), acetate buffer (red line; 10 m m , pH 4), and phosphate buffer (blue line; 10 m m . pH 7). Inset: Magnification of the low‐concentration range.

    Journal: ChemistryOpen

    Article Title: Mix‐ ‐Read Determination of Mercury(II) at Trace Levels with Hybrid Mesoporous Silica Materials Incorporating Fluorescent Probes by a Simple Mix‐ ‐Load Technique

    doi: 10.1002/open.201800111

    Figure Lengend Snippet: a) Fluorescence emission spectra ( λ ex =490 nm) of xPRO‐SBA‐ I (0.11 mg mL −1 in Milli‐Q water, pH 7) in the presence of different amounts of Hg II . Inset: Corresponding normalized absorption spectra. b) Corresponding fluorescence enhancement ratio (Δ F / F 0 ) registered at λ =538 nm ( λ ex =490 nm) as a function of Hg II added in Milli‐Q Water (black line, pH 7), acetate buffer (red line; 10 m m , pH 4), and phosphate buffer (blue line; 10 m m . pH 7). Inset: Magnification of the low‐concentration range.

    Article Snippet: Phosphate buffer and acetate buffer solutions (10 mm ) were prepared with ultrapure reagent water, which was obtained by running demineralized water (by ion exchange) through a Milli‐Q ultrapure water purification system (Millipore Synthesis A10).

    Techniques: Fluorescence, Concentration Assay

    Absorption spectra of BODIPY dye I (1.1 μ m ; black lines) in a) water and c) MeCN in the absence and in the presence of 1 ppm of cations Hg II (green line), Ag I (red line), Ni II (blue line), and Cu II (orange line; the bleaching behavior of Cu II , which only occurs in MeCN yet not in H 2 O or aq. MeCN, was observed before, see Ref. 41 ). Corresponding fluorescence emission spectra ( λ ex =490 nm) of I (1.1 μ m ) in b) Milli‐Q water at pH 7 and d) MeCN. Inset bottom: Corresponding photographs under UV light ( λ ex =365 nm) of an initial solution of BODIPY I dye (1.1 μ m ) in the presence of (from left to right) 1 ppm of Hg II , Ag I , Ni II , and Cu II .

    Journal: ChemistryOpen

    Article Title: Mix‐ ‐Read Determination of Mercury(II) at Trace Levels with Hybrid Mesoporous Silica Materials Incorporating Fluorescent Probes by a Simple Mix‐ ‐Load Technique

    doi: 10.1002/open.201800111

    Figure Lengend Snippet: Absorption spectra of BODIPY dye I (1.1 μ m ; black lines) in a) water and c) MeCN in the absence and in the presence of 1 ppm of cations Hg II (green line), Ag I (red line), Ni II (blue line), and Cu II (orange line; the bleaching behavior of Cu II , which only occurs in MeCN yet not in H 2 O or aq. MeCN, was observed before, see Ref. 41 ). Corresponding fluorescence emission spectra ( λ ex =490 nm) of I (1.1 μ m ) in b) Milli‐Q water at pH 7 and d) MeCN. Inset bottom: Corresponding photographs under UV light ( λ ex =365 nm) of an initial solution of BODIPY I dye (1.1 μ m ) in the presence of (from left to right) 1 ppm of Hg II , Ag I , Ni II , and Cu II .

    Article Snippet: Phosphate buffer and acetate buffer solutions (10 mm ) were prepared with ultrapure reagent water, which was obtained by running demineralized water (by ion exchange) through a Milli‐Q ultrapure water purification system (Millipore Synthesis A10).

    Techniques: Fluorescence