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AMS Biotechnology miliunits heparinase iii
Heparan sulfates are crucial for SARS-CoV-2 binding and infection. ( A-B ) Huh 7.5 cells were preincubated with neutralizing antibody toACE2 and SARS-CoV-2 pseudovirus was pre-incubated with mAb COVA1-18, COVA1-21 and COVA2-15 or UF heparin (250IU). Cells were infected with SARS-CoV-2 pseudovirus and binding was determined by ELISA. ( C ) Heparan sulfates were removed from Huh 7.5 cells by <t>heparinase</t> treatment and SARS-CoV-2 pseudovirus binding was determined. Error bars are the mean ± SEM from <t>three</t> independent experiments. ( D ) Huh 7.5 cells were infected with SARS-CoV-2 pseudovirus and infection was measured after 5 days of culture by luciferase assay. Virus was pretreated with heparin (2501U). Data show the mean values and error bars are the SEM. Statistical analysis was performed using ( A ) ordinary one-way ANOVAwith Tukey multiple-comparison test. ***p= 0.0006, ***p= 0.0005, **p= 0.0018, **p= 0.0036 (n = 3), ( B ) **p= 0.0010, *p= 0.0255 (n=4), ( C ) two-tailed, unpaired Student’s t-test with Welch’s correction **p= 0.0012 (n = 3), ( D ) ordinary one-way ANOVAwith Tukey multiple-comparison test. **p= 0.0085 (n=6 measured in triplicate). RLU: relative light units.
Miliunits Heparinase Iii, supplied by AMS Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
Price from $9.99 to $1999.99
miliunits heparinase iii - by Bioz Stars, 2020-11
94/100 stars

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Article Title: SARS-CoV-2 Infection and Transmission Depends on Heparan Sulfates and Is Blocked by Low Molecular Weight Heparins

Journal: bioRxiv

doi: 10.1101/2020.08.18.255810

Heparan sulfates are crucial for SARS-CoV-2 binding and infection. ( A-B ) Huh 7.5 cells were preincubated with neutralizing antibody toACE2 and SARS-CoV-2 pseudovirus was pre-incubated with mAb COVA1-18, COVA1-21 and COVA2-15 or UF heparin (250IU). Cells were infected with SARS-CoV-2 pseudovirus and binding was determined by ELISA. ( C ) Heparan sulfates were removed from Huh 7.5 cells by heparinase treatment and SARS-CoV-2 pseudovirus binding was determined. Error bars are the mean ± SEM from three independent experiments. ( D ) Huh 7.5 cells were infected with SARS-CoV-2 pseudovirus and infection was measured after 5 days of culture by luciferase assay. Virus was pretreated with heparin (2501U). Data show the mean values and error bars are the SEM. Statistical analysis was performed using ( A ) ordinary one-way ANOVAwith Tukey multiple-comparison test. ***p= 0.0006, ***p= 0.0005, **p= 0.0018, **p= 0.0036 (n = 3), ( B ) **p= 0.0010, *p= 0.0255 (n=4), ( C ) two-tailed, unpaired Student’s t-test with Welch’s correction **p= 0.0012 (n = 3), ( D ) ordinary one-way ANOVAwith Tukey multiple-comparison test. **p= 0.0085 (n=6 measured in triplicate). RLU: relative light units.
Figure Legend Snippet: Heparan sulfates are crucial for SARS-CoV-2 binding and infection. ( A-B ) Huh 7.5 cells were preincubated with neutralizing antibody toACE2 and SARS-CoV-2 pseudovirus was pre-incubated with mAb COVA1-18, COVA1-21 and COVA2-15 or UF heparin (250IU). Cells were infected with SARS-CoV-2 pseudovirus and binding was determined by ELISA. ( C ) Heparan sulfates were removed from Huh 7.5 cells by heparinase treatment and SARS-CoV-2 pseudovirus binding was determined. Error bars are the mean ± SEM from three independent experiments. ( D ) Huh 7.5 cells were infected with SARS-CoV-2 pseudovirus and infection was measured after 5 days of culture by luciferase assay. Virus was pretreated with heparin (2501U). Data show the mean values and error bars are the SEM. Statistical analysis was performed using ( A ) ordinary one-way ANOVAwith Tukey multiple-comparison test. ***p= 0.0006, ***p= 0.0005, **p= 0.0018, **p= 0.0036 (n = 3), ( B ) **p= 0.0010, *p= 0.0255 (n=4), ( C ) two-tailed, unpaired Student’s t-test with Welch’s correction **p= 0.0012 (n = 3), ( D ) ordinary one-way ANOVAwith Tukey multiple-comparison test. **p= 0.0085 (n=6 measured in triplicate). RLU: relative light units.

Techniques Used: Binding Assay, Infection, Incubation, Enzyme-linked Immunosorbent Assay, Luciferase, Two Tailed Test

( A ) ACE2 cell surface expression on Namatwa cell line, DCs, LCs and Huh 7.5. Representative data for an experiment repeated more than three times with similar results. ( B ) Huh7.5 were left untreated or treated with heparinase for 1 hand heparan sulfate or digested heparan sulfate expression was determined by flow cytometry. One representative donor out of 3 is depicted. ( C ) Cell surface expression of ACE2 on 293T and Caco2 cell lines was determined by real-time PCR. ( D ) LC were stained with antibodies against CD207 and CD1 a and analysed by flow cytometry. The histogram shows the cell surface expression of the receptor. Representative data for an experiment repeated more than three times with similar results. Data show the mean values and error bars are the SEM. Statistical analysis was performed using ( A ) ordinary one-way ANOVA with Tukey’s multiple-comparison test. ****p
Figure Legend Snippet: ( A ) ACE2 cell surface expression on Namatwa cell line, DCs, LCs and Huh 7.5. Representative data for an experiment repeated more than three times with similar results. ( B ) Huh7.5 were left untreated or treated with heparinase for 1 hand heparan sulfate or digested heparan sulfate expression was determined by flow cytometry. One representative donor out of 3 is depicted. ( C ) Cell surface expression of ACE2 on 293T and Caco2 cell lines was determined by real-time PCR. ( D ) LC were stained with antibodies against CD207 and CD1 a and analysed by flow cytometry. The histogram shows the cell surface expression of the receptor. Representative data for an experiment repeated more than three times with similar results. Data show the mean values and error bars are the SEM. Statistical analysis was performed using ( A ) ordinary one-way ANOVA with Tukey’s multiple-comparison test. ****p

Techniques Used: Expressing, Flow Cytometry, Real-time Polymerase Chain Reaction, Staining

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Article Title: SARS-CoV-2 Infection and Transmission Depends on Heparan Sulfates and Is Blocked by Low Molecular Weight Heparins
Article Snippet: .. Biosynthesis inhibition and enzymatic treatmentHuH7.5 cells were treated in D-PBS/0.25% BSA with 46 miliunits heparinase III (Amsbio) for 1 hour at 37°C, washed and used in subsequent experiments. .. Enzymatic digestion was verified by flow cytometry using antibodies directed against heparan sulfates and digested heparan sulfates.

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    AMS Biotechnology miliunits heparinase iii
    Heparan sulfates are crucial for SARS-CoV-2 binding and infection. ( A-B ) Huh 7.5 cells were preincubated with neutralizing antibody toACE2 and SARS-CoV-2 pseudovirus was pre-incubated with mAb COVA1-18, COVA1-21 and COVA2-15 or UF heparin (250IU). Cells were infected with SARS-CoV-2 pseudovirus and binding was determined by ELISA. ( C ) Heparan sulfates were removed from Huh 7.5 cells by <t>heparinase</t> treatment and SARS-CoV-2 pseudovirus binding was determined. Error bars are the mean ± SEM from <t>three</t> independent experiments. ( D ) Huh 7.5 cells were infected with SARS-CoV-2 pseudovirus and infection was measured after 5 days of culture by luciferase assay. Virus was pretreated with heparin (2501U). Data show the mean values and error bars are the SEM. Statistical analysis was performed using ( A ) ordinary one-way ANOVAwith Tukey multiple-comparison test. ***p= 0.0006, ***p= 0.0005, **p= 0.0018, **p= 0.0036 (n = 3), ( B ) **p= 0.0010, *p= 0.0255 (n=4), ( C ) two-tailed, unpaired Student’s t-test with Welch’s correction **p= 0.0012 (n = 3), ( D ) ordinary one-way ANOVAwith Tukey multiple-comparison test. **p= 0.0085 (n=6 measured in triplicate). RLU: relative light units.
    Miliunits Heparinase Iii, supplied by AMS Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/miliunits heparinase iii/product/AMS Biotechnology
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    miliunits heparinase iii - by Bioz Stars, 2020-11
    94/100 stars
      Buy from Supplier

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    Heparan sulfates are crucial for SARS-CoV-2 binding and infection. ( A-B ) Huh 7.5 cells were preincubated with neutralizing antibody toACE2 and SARS-CoV-2 pseudovirus was pre-incubated with mAb COVA1-18, COVA1-21 and COVA2-15 or UF heparin (250IU). Cells were infected with SARS-CoV-2 pseudovirus and binding was determined by ELISA. ( C ) Heparan sulfates were removed from Huh 7.5 cells by heparinase treatment and SARS-CoV-2 pseudovirus binding was determined. Error bars are the mean ± SEM from three independent experiments. ( D ) Huh 7.5 cells were infected with SARS-CoV-2 pseudovirus and infection was measured after 5 days of culture by luciferase assay. Virus was pretreated with heparin (2501U). Data show the mean values and error bars are the SEM. Statistical analysis was performed using ( A ) ordinary one-way ANOVAwith Tukey multiple-comparison test. ***p= 0.0006, ***p= 0.0005, **p= 0.0018, **p= 0.0036 (n = 3), ( B ) **p= 0.0010, *p= 0.0255 (n=4), ( C ) two-tailed, unpaired Student’s t-test with Welch’s correction **p= 0.0012 (n = 3), ( D ) ordinary one-way ANOVAwith Tukey multiple-comparison test. **p= 0.0085 (n=6 measured in triplicate). RLU: relative light units.

    Journal: bioRxiv

    Article Title: SARS-CoV-2 Infection and Transmission Depends on Heparan Sulfates and Is Blocked by Low Molecular Weight Heparins

    doi: 10.1101/2020.08.18.255810

    Figure Lengend Snippet: Heparan sulfates are crucial for SARS-CoV-2 binding and infection. ( A-B ) Huh 7.5 cells were preincubated with neutralizing antibody toACE2 and SARS-CoV-2 pseudovirus was pre-incubated with mAb COVA1-18, COVA1-21 and COVA2-15 or UF heparin (250IU). Cells were infected with SARS-CoV-2 pseudovirus and binding was determined by ELISA. ( C ) Heparan sulfates were removed from Huh 7.5 cells by heparinase treatment and SARS-CoV-2 pseudovirus binding was determined. Error bars are the mean ± SEM from three independent experiments. ( D ) Huh 7.5 cells were infected with SARS-CoV-2 pseudovirus and infection was measured after 5 days of culture by luciferase assay. Virus was pretreated with heparin (2501U). Data show the mean values and error bars are the SEM. Statistical analysis was performed using ( A ) ordinary one-way ANOVAwith Tukey multiple-comparison test. ***p= 0.0006, ***p= 0.0005, **p= 0.0018, **p= 0.0036 (n = 3), ( B ) **p= 0.0010, *p= 0.0255 (n=4), ( C ) two-tailed, unpaired Student’s t-test with Welch’s correction **p= 0.0012 (n = 3), ( D ) ordinary one-way ANOVAwith Tukey multiple-comparison test. **p= 0.0085 (n=6 measured in triplicate). RLU: relative light units.

    Article Snippet: Biosynthesis inhibition and enzymatic treatmentHuH7.5 cells were treated in D-PBS/0.25% BSA with 46 miliunits heparinase III (Amsbio) for 1 hour at 37°C, washed and used in subsequent experiments.

    Techniques: Binding Assay, Infection, Incubation, Enzyme-linked Immunosorbent Assay, Luciferase, Two Tailed Test

    ( A ) ACE2 cell surface expression on Namatwa cell line, DCs, LCs and Huh 7.5. Representative data for an experiment repeated more than three times with similar results. ( B ) Huh7.5 were left untreated or treated with heparinase for 1 hand heparan sulfate or digested heparan sulfate expression was determined by flow cytometry. One representative donor out of 3 is depicted. ( C ) Cell surface expression of ACE2 on 293T and Caco2 cell lines was determined by real-time PCR. ( D ) LC were stained with antibodies against CD207 and CD1 a and analysed by flow cytometry. The histogram shows the cell surface expression of the receptor. Representative data for an experiment repeated more than three times with similar results. Data show the mean values and error bars are the SEM. Statistical analysis was performed using ( A ) ordinary one-way ANOVA with Tukey’s multiple-comparison test. ****p

    Journal: bioRxiv

    Article Title: SARS-CoV-2 Infection and Transmission Depends on Heparan Sulfates and Is Blocked by Low Molecular Weight Heparins

    doi: 10.1101/2020.08.18.255810

    Figure Lengend Snippet: ( A ) ACE2 cell surface expression on Namatwa cell line, DCs, LCs and Huh 7.5. Representative data for an experiment repeated more than three times with similar results. ( B ) Huh7.5 were left untreated or treated with heparinase for 1 hand heparan sulfate or digested heparan sulfate expression was determined by flow cytometry. One representative donor out of 3 is depicted. ( C ) Cell surface expression of ACE2 on 293T and Caco2 cell lines was determined by real-time PCR. ( D ) LC were stained with antibodies against CD207 and CD1 a and analysed by flow cytometry. The histogram shows the cell surface expression of the receptor. Representative data for an experiment repeated more than three times with similar results. Data show the mean values and error bars are the SEM. Statistical analysis was performed using ( A ) ordinary one-way ANOVA with Tukey’s multiple-comparison test. ****p

    Article Snippet: Biosynthesis inhibition and enzymatic treatmentHuH7.5 cells were treated in D-PBS/0.25% BSA with 46 miliunits heparinase III (Amsbio) for 1 hour at 37°C, washed and used in subsequent experiments.

    Techniques: Expressing, Flow Cytometry, Real-time Polymerase Chain Reaction, Staining