Structured Review

BioExpress microtubes
Microtubes, supplied by BioExpress, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/microtubes/product/BioExpress
Average 92 stars, based on 4 article reviews
Price from $9.99 to $1999.99
microtubes - by Bioz Stars, 2020-09
92/100 stars

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Related Articles

Flow Cytometry:

Article Title: In vitro Reconstitution of B Cell Receptor Antigen Interactions to Evaluate Potential Vaccine Candidates
Article Snippet: .. Nitrogen cylinder with pressure gauge and regulator Manifold for distributing nitrogen flow for evaporation Liquid nitrogen dewar (Finemech, cat. no. KGW-10215) −2.0 ml microtubes (Bioexpress, cat. no. C-3261–1) Extruder apparatus with 1000 μl Hamilton syringes (Avanti Polar Lipids, cat. no 610000) Extruder filter supports (Whatman, cat. no. 230300) −0.1 μm extruder filter (Whatman, cat. no. 800309) Extruder heating block (Bioexpress, cat. no. D-2000–1B) Beckman Optima MAX ultracentrifuge Thinwall, Ultra-Clear™, 11 × 60 mm ultra centrifuge tubes (Beckman, cat. no. 335649) TH660 rotor (Thermo Scientific, cat. no. 10502) Lipid glassware (e.g. Wheaton® Scintillation Vials with polypropylene cap) −25-gauge (25-G) Needles (BD Medical, cat. no. 305127) −5-ml Plastic syringes (BD Medical, cat. no. 301027) Vortexer (Scientific Industries, cat. no. SI-0236, or equivalent) Slide-A-Lyzer Dialysis Cassette, 10,000 MWCO, (Thermo Scientific, cat. no. 66380) .. −0.45 μM syringe filters (Millipore, cat. no. SLHV033RS) −10 cm culture dishes (Corning, cat. no. 430167) −96 well culture dishes (Corning, cat. no. 353072) −48 well culture dishes (Corning, cat. no. 3548) −24 well culture dishes (Corning, cat. no. 3524) −12 well culture dishes (Corning, cat. no. 3512) −6 well plates (Corning, cat. no. 3506) T25 culture flask (Corning, cat. no. 3056) T75 culture flask (Corning, cat. no. 430641U) −0.22 μM filter (Corning, cat. no. 430758) FACS tubes (Corning, cat. no 353054) −96 well plates for luminescence (PerkinElmer, cat. no. 6005030) Cell Freezing container (Thermo Scientific, cat. no. 5100–0001) Cell culture cryotubes (Thermo Scientific, cat. no. 374081)

Transferring:

Article Title: Transient partial permeabilization with saponin enables cellular barcoding prior to surface marker staining
Article Snippet: .. Barcoding was performed on approximately 1 million fixed cells placed into racked, 1.1mL microtubes (BioExpress, Kaysville, UT, USA) using a multichannel pipette and a multichannel aspirator. .. Fixed cells were washed once in CSM and then washed once in PBS, followed by a second wash in PBS plus 0.02% saponin (Sigma-Aldrich, St. Louis, MO, USA), or PBS plus the indicated saponin concentration for titration experiments (all pre-barcoding saponin washes were performed at 4°C).

Size-exclusion Chromatography:

Article Title: Running throughout middle-age improves memory function, hippocampal neurogenesis and BDNF levels in female C57Bl/6J mice
Article Snippet: .. To assay mature BDNF peptide levels, hippocampal tissue was homogenized in 400 μl of a 1 X RIPA buffer containing protease inhibitors (Complete Mini, Roche Diagnostics) using pestles and microtubes (ISC BioExpress) and then sonicated for 10 sec (Ultrasonic Processor, Model GE70). .. The protein concentrations were then measured using the Bradford method (Bio-Rad).

Article Title: Running is the neurogenic and neurotrophic stimulus in environmental enrichment
Article Snippet: .. To assay mature BDNF peptide levels, hippocampal tissue was homogenized in 500 µL of the 1× RIPA buffer containing protease inhibitors (Complete Mini, Roche Diagnostics) using pestles and microtubes (ISC BioExpress) and then sonicated with four pulses of 10 sec at scale 4 (Ultrosonic Processor, Model GE70) at room temperature. ..

Blocking Assay:

Article Title: In vitro Reconstitution of B Cell Receptor Antigen Interactions to Evaluate Potential Vaccine Candidates
Article Snippet: .. Nitrogen cylinder with pressure gauge and regulator Manifold for distributing nitrogen flow for evaporation Liquid nitrogen dewar (Finemech, cat. no. KGW-10215) −2.0 ml microtubes (Bioexpress, cat. no. C-3261–1) Extruder apparatus with 1000 μl Hamilton syringes (Avanti Polar Lipids, cat. no 610000) Extruder filter supports (Whatman, cat. no. 230300) −0.1 μm extruder filter (Whatman, cat. no. 800309) Extruder heating block (Bioexpress, cat. no. D-2000–1B) Beckman Optima MAX ultracentrifuge Thinwall, Ultra-Clear™, 11 × 60 mm ultra centrifuge tubes (Beckman, cat. no. 335649) TH660 rotor (Thermo Scientific, cat. no. 10502) Lipid glassware (e.g. Wheaton® Scintillation Vials with polypropylene cap) −25-gauge (25-G) Needles (BD Medical, cat. no. 305127) −5-ml Plastic syringes (BD Medical, cat. no. 301027) Vortexer (Scientific Industries, cat. no. SI-0236, or equivalent) Slide-A-Lyzer Dialysis Cassette, 10,000 MWCO, (Thermo Scientific, cat. no. 66380) .. −0.45 μM syringe filters (Millipore, cat. no. SLHV033RS) −10 cm culture dishes (Corning, cat. no. 430167) −96 well culture dishes (Corning, cat. no. 353072) −48 well culture dishes (Corning, cat. no. 3548) −24 well culture dishes (Corning, cat. no. 3524) −12 well culture dishes (Corning, cat. no. 3512) −6 well plates (Corning, cat. no. 3506) T25 culture flask (Corning, cat. no. 3056) T75 culture flask (Corning, cat. no. 430641U) −0.22 μM filter (Corning, cat. no. 430758) FACS tubes (Corning, cat. no 353054) −96 well plates for luminescence (PerkinElmer, cat. no. 6005030) Cell Freezing container (Thermo Scientific, cat. no. 5100–0001) Cell culture cryotubes (Thermo Scientific, cat. no. 374081)

Evaporation:

Article Title: In vitro Reconstitution of B Cell Receptor Antigen Interactions to Evaluate Potential Vaccine Candidates
Article Snippet: .. Nitrogen cylinder with pressure gauge and regulator Manifold for distributing nitrogen flow for evaporation Liquid nitrogen dewar (Finemech, cat. no. KGW-10215) −2.0 ml microtubes (Bioexpress, cat. no. C-3261–1) Extruder apparatus with 1000 μl Hamilton syringes (Avanti Polar Lipids, cat. no 610000) Extruder filter supports (Whatman, cat. no. 230300) −0.1 μm extruder filter (Whatman, cat. no. 800309) Extruder heating block (Bioexpress, cat. no. D-2000–1B) Beckman Optima MAX ultracentrifuge Thinwall, Ultra-Clear™, 11 × 60 mm ultra centrifuge tubes (Beckman, cat. no. 335649) TH660 rotor (Thermo Scientific, cat. no. 10502) Lipid glassware (e.g. Wheaton® Scintillation Vials with polypropylene cap) −25-gauge (25-G) Needles (BD Medical, cat. no. 305127) −5-ml Plastic syringes (BD Medical, cat. no. 301027) Vortexer (Scientific Industries, cat. no. SI-0236, or equivalent) Slide-A-Lyzer Dialysis Cassette, 10,000 MWCO, (Thermo Scientific, cat. no. 66380) .. −0.45 μM syringe filters (Millipore, cat. no. SLHV033RS) −10 cm culture dishes (Corning, cat. no. 430167) −96 well culture dishes (Corning, cat. no. 353072) −48 well culture dishes (Corning, cat. no. 3548) −24 well culture dishes (Corning, cat. no. 3524) −12 well culture dishes (Corning, cat. no. 3512) −6 well plates (Corning, cat. no. 3506) T25 culture flask (Corning, cat. no. 3056) T75 culture flask (Corning, cat. no. 430641U) −0.22 μM filter (Corning, cat. no. 430758) FACS tubes (Corning, cat. no 353054) −96 well plates for luminescence (PerkinElmer, cat. no. 6005030) Cell Freezing container (Thermo Scientific, cat. no. 5100–0001) Cell culture cryotubes (Thermo Scientific, cat. no. 374081)

Cell Culture:

Article Title: Comparison of Chondrogenic Potential in Equine Mesenchymal Stromal Cells Derived from Adipose Tissue and Bone Marrow
Article Snippet: .. Cells were trypsinized and aliquots of 0.25 × 106 cells (P2) were placed into racked microtubes (ISC BioExpress, Kaysville, UT) tubes and centrifuged for 5 minutes at 240 × g. The resulting pellets were then cultured and induced into chondrogenesis using DMEM/high glucose (10%), 1% antibiotic/antifungal solution, dexamethasone (100 nM), ascorbic acid 2-phosphate (50g/mL) and ITS+ (culture supplement containing bovine insulin, transferrin, selenous acid, linoleic acid, and BSA; BD Biosciences, Bedford, MA) with or without TGFβ3 (human recombinant TGFβ3 , R & D Systems Inc., Minneapolis, MN) (10 ng/mL) and BMP factor-6 (human recombinant BMP-6, R & D Systems Inc.) (10 ng/mL). .. Pellets were either papain digested for quantification of total DNA and glycosaminoglycans (GAG) or fixed in 10% formalin and subsequently embedded in paraffin for staining and evaluation with light microscopy.

Lysis:

Article Title: Association of time-dependent changes in mu opioid receptor mRNA, but not BDNF, TrkB, or MeCP2 mRNA and protein expression in the rat nucleus accumbens with incubation of heroin craving
Article Snippet: .. Samples were homogenized using pestles and microtubes (ISC BioExpress) in 100 μl of 1× RIPA lysis buffer (Cell Signaling, Danvers, MA) containing inhibitor cocktails for phosphatases (PhosSTOP, Roche Applied Sciences, Indiananapolis, IN) and proteases (cOmplete, Ultra tablets, EDTA-free, Roche Applied Sciences) and subsequently sonicated for 10 s. Samples were then reduced with 20 mM DTT at 45 °C for 90 min and centrifuged at room temperature for 10 min. Supernatants were collected and protein concentrations were measured using the Bradford method (Bio-Rad Laboratories, Hercules, CA). .. Samples were diluted to a final concentration of 1 μg/μl with the lysis buffer and the 4× LDS NuPAGE sample buffer (Invitrogen-Life Technologies).

Sonication:

Article Title: Association of time-dependent changes in mu opioid receptor mRNA, but not BDNF, TrkB, or MeCP2 mRNA and protein expression in the rat nucleus accumbens with incubation of heroin craving
Article Snippet: .. Samples were homogenized using pestles and microtubes (ISC BioExpress) in 100 μl of 1× RIPA lysis buffer (Cell Signaling, Danvers, MA) containing inhibitor cocktails for phosphatases (PhosSTOP, Roche Applied Sciences, Indiananapolis, IN) and proteases (cOmplete, Ultra tablets, EDTA-free, Roche Applied Sciences) and subsequently sonicated for 10 s. Samples were then reduced with 20 mM DTT at 45 °C for 90 min and centrifuged at room temperature for 10 min. Supernatants were collected and protein concentrations were measured using the Bradford method (Bio-Rad Laboratories, Hercules, CA). .. Samples were diluted to a final concentration of 1 μg/μl with the lysis buffer and the 4× LDS NuPAGE sample buffer (Invitrogen-Life Technologies).

Article Title: Running throughout middle-age improves memory function, hippocampal neurogenesis and BDNF levels in female C57Bl/6J mice
Article Snippet: .. To assay mature BDNF peptide levels, hippocampal tissue was homogenized in 400 μl of a 1 X RIPA buffer containing protease inhibitors (Complete Mini, Roche Diagnostics) using pestles and microtubes (ISC BioExpress) and then sonicated for 10 sec (Ultrasonic Processor, Model GE70). .. The protein concentrations were then measured using the Bradford method (Bio-Rad).

Article Title: Prolonged Running, not Fluoxetine Treatment, Increases Neurogenesis, but does not Alter Neuropathology, in the 3xTg Mouse Model of Alzheimer’s Disease
Article Snippet: .. To measure mature BDNF peptide levels, hippocampal tissue was homogenized in 400 µl of the 1X RIPA buffer containing protease inhibitors (Complete Mini, Roche Diagnostics) using pestles and microtubes (ISC BioExpress) and then sonicated with four pulses of 10 s at scale 4 (Ultrasonic Processor, Model GE70) at RT. ..

Article Title: Running is the neurogenic and neurotrophic stimulus in environmental enrichment
Article Snippet: .. To assay mature BDNF peptide levels, hippocampal tissue was homogenized in 500 µL of the 1× RIPA buffer containing protease inhibitors (Complete Mini, Roche Diagnostics) using pestles and microtubes (ISC BioExpress) and then sonicated with four pulses of 10 sec at scale 4 (Ultrosonic Processor, Model GE70) at room temperature. ..

Recombinant:

Article Title: Comparison of Chondrogenic Potential in Equine Mesenchymal Stromal Cells Derived from Adipose Tissue and Bone Marrow
Article Snippet: .. Cells were trypsinized and aliquots of 0.25 × 106 cells (P2) were placed into racked microtubes (ISC BioExpress, Kaysville, UT) tubes and centrifuged for 5 minutes at 240 × g. The resulting pellets were then cultured and induced into chondrogenesis using DMEM/high glucose (10%), 1% antibiotic/antifungal solution, dexamethasone (100 nM), ascorbic acid 2-phosphate (50g/mL) and ITS+ (culture supplement containing bovine insulin, transferrin, selenous acid, linoleic acid, and BSA; BD Biosciences, Bedford, MA) with or without TGFβ3 (human recombinant TGFβ3 , R & D Systems Inc., Minneapolis, MN) (10 ng/mL) and BMP factor-6 (human recombinant BMP-6, R & D Systems Inc.) (10 ng/mL). .. Pellets were either papain digested for quantification of total DNA and glycosaminoglycans (GAG) or fixed in 10% formalin and subsequently embedded in paraffin for staining and evaluation with light microscopy.

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  • 92
    BioExpress microtubes
    Microtubes, supplied by BioExpress, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/microtubes/product/BioExpress
    Average 92 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    microtubes - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    85
    BioExpress genemate slick low adhesion microtubes
    Genemate Slick Low Adhesion Microtubes, supplied by BioExpress, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/genemate slick low adhesion microtubes/product/BioExpress
    Average 85 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    genemate slick low adhesion microtubes - by Bioz Stars, 2020-09
    85/100 stars
      Buy from Supplier

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