microtube  (Thermo Fisher)


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    Name:
    Microtubes for Microtube System
    Description:
    The 1 1 mL microtubes resemble our 1 mL pipette tips except that they have round bottoms Like a box of 1 mL pipette tips the racked microtubes match multi channel pipettors providing convenience for a variety of reagent and sample dispensing procedures Features • Available individually or in an 8 x 12 microtitration plate format • The 8 x 12 format simplifies and speeds applications in which a multi channel pipettor is used • Format is compatible with robotic workstations • MicroTube System can be centrifuged Related Products 1 1 mL Microtube System Racked In Line Caps for 1 1 mL Microtube System
    Catalog Number:
    15086
    Price:
    None
    Applications:
    Protein Assays and Analysis|Protein Biology
    Category:
    Lab Supplies Plastics Glassware
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    Structured Review

    Thermo Fisher microtube
    The 1 1 mL microtubes resemble our 1 mL pipette tips except that they have round bottoms Like a box of 1 mL pipette tips the racked microtubes match multi channel pipettors providing convenience for a variety of reagent and sample dispensing procedures Features • Available individually or in an 8 x 12 microtitration plate format • The 8 x 12 format simplifies and speeds applications in which a multi channel pipettor is used • Format is compatible with robotic workstations • MicroTube System can be centrifuged Related Products 1 1 mL Microtube System Racked In Line Caps for 1 1 mL Microtube System
    https://www.bioz.com/result/microtube/product/Thermo Fisher
    Average 99 stars, based on 17 article reviews
    Price from $9.99 to $1999.99
    microtube - by Bioz Stars, 2020-09
    99/100 stars

    Images

    Related Articles

    Polymerase Chain Reaction:

    Article Title: A new lizard malaria parasite Plasmodium intabazwe n. sp. (Apicomplexa: Haemospororida: Plasmodiidae) in the Afromontane Pseudocordylus melanotus (Sauria: Cordylidae) with a review of African saurian malaria parasites
    Article Snippet: .. The PCR conditions were as follows: initial denaturation at 94 °C for 3 min, followed by 35 cycles, entailing a 94 °C denaturation for 30 s, annealing at 50 °C for 30 s with an end extension at 72 °C for 45 s, and following the cycles a final extension of 72 °C for 10 min. All PCR reactions were performed in a 25 μl volume microtube, using 12.5 μl Thermo Scientific DreamTaq PCR master mix (2×) (2× DreamTaq buffer, 0.4 mM of each dNTP, and 4 mM MgCl2), 1.25 μl of each primer, and at least 25 ng of genomic DNA for the first PCR, and 1 μl of the PCR product from the initial PCR for the second PCR. .. The remaining volume was made up of PCR-grade nuclease-free water (Thermo Scientific, Vilnius, Lithuania).

    Incubation:

    Article Title: Sustained release of BMP-2 in a lipid-based microtube vehicle
    Article Snippet: .. To monitor the degradation, microtubes were incubated at 37°C in 500 μl of phosphate-buffered saline (PBS) or minimal essential medium (α-MEM; Invitrogen, Carlsbad, CA, USA) with 15% fetal calf serum (FBS; Atlanta Biologicals, Atlanta, GA, USA), 2 mM L-glutamine, 100 units of penicillin and 100 μg of streptomycin (Invitrogen). .. At various time points microtube samples were collected and theit lengths measured using the light microscope.

    Article Title: Conjugative Selectivity of Plasmids Is Affected by Coexisting Recipient Candidates
    Article Snippet: .. After suspension of the cells in the culture supernatants, the mating mixtures were sealed in 2-ml microtubes with a gas-permeable adhesive seal (Thermo Fisher Scientific) and were incubated at 30°C for 3 h. TFs were calculated as described under “Mating assay” above. ..

    Article Title: Conjugative Selectivity of Plasmids Is Affected by Coexisting Recipient Candidates
    Article Snippet: .. Donor and recipient cells were mixed in 2-ml microtubes sealed with a gas-permeable adhesive seal (Thermo Fisher Scientific, Waltham, MA, USA) and incubated for 1, 3, or 16 h at 30°C for liquid mating. .. A mixture of donor and recipient cells was transferred onto a 0.22-μm-pore-size membrane filter (Millipore, Billerica, MA, USA) using glass microanalysis filter holders and filtering flasks (Millipore) for filter mating.

    Activity Assay:

    Article Title: Biodegradable Porous Silk Microtubes for Tissue Vascularization
    Article Snippet: .. To assess changes and differences in cellular activity, the different microtubes were assayed every 3 days using Alamar Blue (Invitrogen Corp, Grand Island, NY). .. Briefly, a 9:1 (v/v) dilution of Alamar Blue to cell culture medium was added directly to the samples and then incubated for 4 hours.

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  • 99
    Thermo Fisher microtubes
    Transfer frequency (TF) of each plasmid in the 1:1 mating (upper panels) and 1:2 mating (lower panels) assays of liquid mating. Plasmid-harboring strains of Pseudomonas putida (A) or P. resinovorans (B) were used as donors. As the recipient strain(s), P. putida (or P. resinovorans ) and both strains were used in 1:1 and 1:2 mating assays, respectively. Cell mixtures were incubated in <t>microtubes</t> containing LB for 3 h at 30°C to allow mating. Bars show the mean TFs (transconjugants/donor) calculated from triplicate assays (shown by white diamonds). White bars show TFs of plasmids to P. putida , and black bars show TFs of plasmids to P. resinovorans . All experiments were performed twice, and their reproducibility was confirmed. Asterisks indicate significant differences between two conditions as assessed by Student's t test ( P
    Microtubes, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/microtubes/product/Thermo Fisher
    Average 99 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    microtubes - by Bioz Stars, 2020-09
    99/100 stars
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    94
    Thermo Fisher e254a microtubules
    Characterization of EB3 binding to wildtype and GTPase deficient human microtubules. ( a ) Coomassie Blue-stained SDS gel of purified recombinant mGFP-EB3 and Alexa647-labeled SNAP-EB3 (Alexa647-EB3). ( b, c ) Comparative iSCAT/TIRF microscopy images of 20 nM mGFP-EB3 binding to ( b ) unlabeled <t>E254A</t> microtubules (E254A MTs) and ( c ) wildtype microtubules polymerized in the presence of GMPCPP (WT GMPCPP MTs), attached to a Kin1 rigor surface. EB3 binds strongly to E254A microtubules, but not to GMPCPP microtubules. ( d ) Comparative TIRF microscopy images of 25 nM mGFP-EB3 binding to unlabeled E254A microtubules polymerized in the presence of GTP (E254A GTP MTs, ) or in the presence of GMPCPP (E254A GMPCPP MTs). Microtubules were attached to a Kin1 rigor surface. EB3 binds strongly to E254A microtubules polymerized in the presence of GTP, but not to E254A microtubules polymerized in the presence of GMPCPP. Two different exposure conditions (50 ms and 2000 ms) are shown for GMPCPP-bound E254A microtubules to visualize the faintly bound mGFP-EB3. ( e ) TIRF microscopy images of 500 nM mGFP-TPX2 micro binding to a mixture of fluorescently labeled GMPCPP-bound porcine brain microtubules (porcine brain GMPCPP MTs, magenta) and unlabeled GTP-bound E254A microtubules (E254A MTs). mGFP-TPX2 micro binds strongly to GMPCPP microtubules (cyan arrowheads) and faintly to GTP-bound E254A microtubules (yellow arrowheads). ( f ) EB3 washout experiment: TIRF microscopy images of mGFP-EB3 at 2.5 nM bound to unlabeled E254A microtubules (E254A MTs) immobilized on a Kin1 rigor surface before (left) and after (right) washing out mGFP-EB3 from the flow chamber. ( g ) Steady-state bleaching curves for 2.5 nM mGFP-EB3 bound to Kin1 rigor surface-immobilized E254A microtubules (E254A MTs), imaged with a 60 ms exposure time and different frame intervals, as indicated. Dashed magenta lines are a fit to the data (Materials and methods) revealing a dwell time of mGFP-EB3 molecules of 83 s (and bleaching times of 45 s, 83 s, and 171 s for imaging with a time lapse of 60 ms (single molecule imaging condition), 100 ms, and 200 ms, respectively). The obtained dwell time and bleaching time for the 60 ms time lapse are consistent with the measured apparent mean dwell time of 1/ (0.029 s −1 ) = 34 s for single mGFP-EB3 molecules on E254A microtubules ( Figure 2k ). This single molecule dwell time is affected by bleaching. Its inverse value is expected to be the sum of the inverse real dwell time and the inverse bleaching time obtained from the steady state bleaching experiment here in ( g ), which is the case. Scale bars as indicated, time is in min:sec.
    E254a Microtubules, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e254a microtubules/product/Thermo Fisher
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    e254a microtubules - by Bioz Stars, 2020-09
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    80
    Thermo Fisher fluorescently labelled microtubules
    Anillin slides actin filaments to maximize their overlap. ( A ) Schematic representation of the experimental setup. The asterisk denotes components used only in experiments with immobilised filaments. ( B ) Intensity-inverted kymograph showing single anillin-GFP molecules diffusing along an actin filament. ( C ) Fluorescence micrograph (top) showing three short mobile actin filaments (indicated by green arrowheads) crosslinked by anillin-GFP (not visualized) to long, sparsely <t>fluorescently</t> labelled, immobilised actin filaments (see Movie S1). Kymographs (bottom) show the diffusion of mobile actin filaments from the micrograph above along the immobilised filaments. ( D ) Time-lapse micrographs (top) and a kymograph (bottom) showing anillin-driven sliding of a mobile actin filament (bright) along an immobilised filament (dim), increasing the overlap between the two actin filaments (see Movie S2). Green and magenta arrowheads indicate the ends of the immobilised and mobile filaments, respectively. This experiment was repeated 5 times (8 events observed) with similar results. ( E ) Time-lapse micrographs showing anillin-driven sliding of two mobile actin filaments along each other, increasing the overlap between the two actin filaments (see Movie S3). Arrowheads indicate the filaments’ ends. This experiment was repeated 15 times (24 events observed) with similar results. ( F ) A typical time trace of the overlap expansion (green dots) reaching an equilibrium value (magenta line represents an exponential fit (Methods) to the data). ( G ) Velocity of the overlap expansion decreases with increasing overlap length. Green points represent an exemplary event, magenta line is a linear fit to the data.
    Fluorescently Labelled Microtubules, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 80/100, based on 0 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fluorescently labelled microtubules/product/Thermo Fisher
    Average 80 stars, based on 1 article reviews
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    Image Search Results


    Transfer frequency (TF) of each plasmid in the 1:1 mating (upper panels) and 1:2 mating (lower panels) assays of liquid mating. Plasmid-harboring strains of Pseudomonas putida (A) or P. resinovorans (B) were used as donors. As the recipient strain(s), P. putida (or P. resinovorans ) and both strains were used in 1:1 and 1:2 mating assays, respectively. Cell mixtures were incubated in microtubes containing LB for 3 h at 30°C to allow mating. Bars show the mean TFs (transconjugants/donor) calculated from triplicate assays (shown by white diamonds). White bars show TFs of plasmids to P. putida , and black bars show TFs of plasmids to P. resinovorans . All experiments were performed twice, and their reproducibility was confirmed. Asterisks indicate significant differences between two conditions as assessed by Student's t test ( P

    Journal: mSphere

    Article Title: Conjugative Selectivity of Plasmids Is Affected by Coexisting Recipient Candidates

    doi: 10.1128/mSphere.00490-18

    Figure Lengend Snippet: Transfer frequency (TF) of each plasmid in the 1:1 mating (upper panels) and 1:2 mating (lower panels) assays of liquid mating. Plasmid-harboring strains of Pseudomonas putida (A) or P. resinovorans (B) were used as donors. As the recipient strain(s), P. putida (or P. resinovorans ) and both strains were used in 1:1 and 1:2 mating assays, respectively. Cell mixtures were incubated in microtubes containing LB for 3 h at 30°C to allow mating. Bars show the mean TFs (transconjugants/donor) calculated from triplicate assays (shown by white diamonds). White bars show TFs of plasmids to P. putida , and black bars show TFs of plasmids to P. resinovorans . All experiments were performed twice, and their reproducibility was confirmed. Asterisks indicate significant differences between two conditions as assessed by Student's t test ( P

    Article Snippet: Donor and recipient cells were mixed in 2-ml microtubes sealed with a gas-permeable adhesive seal (Thermo Fisher Scientific, Waltham, MA, USA) and incubated for 1, 3, or 16 h at 30°C for liquid mating.

    Techniques: Plasmid Preparation, Incubation

    Characterization of EB3 binding to wildtype and GTPase deficient human microtubules. ( a ) Coomassie Blue-stained SDS gel of purified recombinant mGFP-EB3 and Alexa647-labeled SNAP-EB3 (Alexa647-EB3). ( b, c ) Comparative iSCAT/TIRF microscopy images of 20 nM mGFP-EB3 binding to ( b ) unlabeled E254A microtubules (E254A MTs) and ( c ) wildtype microtubules polymerized in the presence of GMPCPP (WT GMPCPP MTs), attached to a Kin1 rigor surface. EB3 binds strongly to E254A microtubules, but not to GMPCPP microtubules. ( d ) Comparative TIRF microscopy images of 25 nM mGFP-EB3 binding to unlabeled E254A microtubules polymerized in the presence of GTP (E254A GTP MTs, ) or in the presence of GMPCPP (E254A GMPCPP MTs). Microtubules were attached to a Kin1 rigor surface. EB3 binds strongly to E254A microtubules polymerized in the presence of GTP, but not to E254A microtubules polymerized in the presence of GMPCPP. Two different exposure conditions (50 ms and 2000 ms) are shown for GMPCPP-bound E254A microtubules to visualize the faintly bound mGFP-EB3. ( e ) TIRF microscopy images of 500 nM mGFP-TPX2 micro binding to a mixture of fluorescently labeled GMPCPP-bound porcine brain microtubules (porcine brain GMPCPP MTs, magenta) and unlabeled GTP-bound E254A microtubules (E254A MTs). mGFP-TPX2 micro binds strongly to GMPCPP microtubules (cyan arrowheads) and faintly to GTP-bound E254A microtubules (yellow arrowheads). ( f ) EB3 washout experiment: TIRF microscopy images of mGFP-EB3 at 2.5 nM bound to unlabeled E254A microtubules (E254A MTs) immobilized on a Kin1 rigor surface before (left) and after (right) washing out mGFP-EB3 from the flow chamber. ( g ) Steady-state bleaching curves for 2.5 nM mGFP-EB3 bound to Kin1 rigor surface-immobilized E254A microtubules (E254A MTs), imaged with a 60 ms exposure time and different frame intervals, as indicated. Dashed magenta lines are a fit to the data (Materials and methods) revealing a dwell time of mGFP-EB3 molecules of 83 s (and bleaching times of 45 s, 83 s, and 171 s for imaging with a time lapse of 60 ms (single molecule imaging condition), 100 ms, and 200 ms, respectively). The obtained dwell time and bleaching time for the 60 ms time lapse are consistent with the measured apparent mean dwell time of 1/ (0.029 s −1 ) = 34 s for single mGFP-EB3 molecules on E254A microtubules ( Figure 2k ). This single molecule dwell time is affected by bleaching. Its inverse value is expected to be the sum of the inverse real dwell time and the inverse bleaching time obtained from the steady state bleaching experiment here in ( g ), which is the case. Scale bars as indicated, time is in min:sec.

    Journal: eLife

    Article Title: The speed of GTP hydrolysis determines GTP cap size and controls microtubule stability

    doi: 10.7554/eLife.51992

    Figure Lengend Snippet: Characterization of EB3 binding to wildtype and GTPase deficient human microtubules. ( a ) Coomassie Blue-stained SDS gel of purified recombinant mGFP-EB3 and Alexa647-labeled SNAP-EB3 (Alexa647-EB3). ( b, c ) Comparative iSCAT/TIRF microscopy images of 20 nM mGFP-EB3 binding to ( b ) unlabeled E254A microtubules (E254A MTs) and ( c ) wildtype microtubules polymerized in the presence of GMPCPP (WT GMPCPP MTs), attached to a Kin1 rigor surface. EB3 binds strongly to E254A microtubules, but not to GMPCPP microtubules. ( d ) Comparative TIRF microscopy images of 25 nM mGFP-EB3 binding to unlabeled E254A microtubules polymerized in the presence of GTP (E254A GTP MTs, ) or in the presence of GMPCPP (E254A GMPCPP MTs). Microtubules were attached to a Kin1 rigor surface. EB3 binds strongly to E254A microtubules polymerized in the presence of GTP, but not to E254A microtubules polymerized in the presence of GMPCPP. Two different exposure conditions (50 ms and 2000 ms) are shown for GMPCPP-bound E254A microtubules to visualize the faintly bound mGFP-EB3. ( e ) TIRF microscopy images of 500 nM mGFP-TPX2 micro binding to a mixture of fluorescently labeled GMPCPP-bound porcine brain microtubules (porcine brain GMPCPP MTs, magenta) and unlabeled GTP-bound E254A microtubules (E254A MTs). mGFP-TPX2 micro binds strongly to GMPCPP microtubules (cyan arrowheads) and faintly to GTP-bound E254A microtubules (yellow arrowheads). ( f ) EB3 washout experiment: TIRF microscopy images of mGFP-EB3 at 2.5 nM bound to unlabeled E254A microtubules (E254A MTs) immobilized on a Kin1 rigor surface before (left) and after (right) washing out mGFP-EB3 from the flow chamber. ( g ) Steady-state bleaching curves for 2.5 nM mGFP-EB3 bound to Kin1 rigor surface-immobilized E254A microtubules (E254A MTs), imaged with a 60 ms exposure time and different frame intervals, as indicated. Dashed magenta lines are a fit to the data (Materials and methods) revealing a dwell time of mGFP-EB3 molecules of 83 s (and bleaching times of 45 s, 83 s, and 171 s for imaging with a time lapse of 60 ms (single molecule imaging condition), 100 ms, and 200 ms, respectively). The obtained dwell time and bleaching time for the 60 ms time lapse are consistent with the measured apparent mean dwell time of 1/ (0.029 s −1 ) = 34 s for single mGFP-EB3 molecules on E254A microtubules ( Figure 2k ). This single molecule dwell time is affected by bleaching. Its inverse value is expected to be the sum of the inverse real dwell time and the inverse bleaching time obtained from the steady state bleaching experiment here in ( g ), which is the case. Scale bars as indicated, time is in min:sec.

    Article Snippet: To test the effect of GMPCPP on mGFP-EB3 binding to E254A microtubules, the E254A tubulin was buffer exchanged from GTP-containing storage buffer to BRB80 containing 0.5 mM GMPCPP using ZebaSpin Desalting Columns (ThermoScientific) and allowed to equilibrate for 30 min in ice.

    Techniques: Binding Assay, Staining, SDS-Gel, Purification, Recombinant, Labeling, Microscopy, Imaging

    Anillin slides actin filaments to maximize their overlap. ( A ) Schematic representation of the experimental setup. The asterisk denotes components used only in experiments with immobilised filaments. ( B ) Intensity-inverted kymograph showing single anillin-GFP molecules diffusing along an actin filament. ( C ) Fluorescence micrograph (top) showing three short mobile actin filaments (indicated by green arrowheads) crosslinked by anillin-GFP (not visualized) to long, sparsely fluorescently labelled, immobilised actin filaments (see Movie S1). Kymographs (bottom) show the diffusion of mobile actin filaments from the micrograph above along the immobilised filaments. ( D ) Time-lapse micrographs (top) and a kymograph (bottom) showing anillin-driven sliding of a mobile actin filament (bright) along an immobilised filament (dim), increasing the overlap between the two actin filaments (see Movie S2). Green and magenta arrowheads indicate the ends of the immobilised and mobile filaments, respectively. This experiment was repeated 5 times (8 events observed) with similar results. ( E ) Time-lapse micrographs showing anillin-driven sliding of two mobile actin filaments along each other, increasing the overlap between the two actin filaments (see Movie S3). Arrowheads indicate the filaments’ ends. This experiment was repeated 15 times (24 events observed) with similar results. ( F ) A typical time trace of the overlap expansion (green dots) reaching an equilibrium value (magenta line represents an exponential fit (Methods) to the data). ( G ) Velocity of the overlap expansion decreases with increasing overlap length. Green points represent an exemplary event, magenta line is a linear fit to the data.

    Journal: bioRxiv

    Article Title: Anillin propels myosin-independent constriction of actin rings

    doi: 10.1101/2020.01.22.915256

    Figure Lengend Snippet: Anillin slides actin filaments to maximize their overlap. ( A ) Schematic representation of the experimental setup. The asterisk denotes components used only in experiments with immobilised filaments. ( B ) Intensity-inverted kymograph showing single anillin-GFP molecules diffusing along an actin filament. ( C ) Fluorescence micrograph (top) showing three short mobile actin filaments (indicated by green arrowheads) crosslinked by anillin-GFP (not visualized) to long, sparsely fluorescently labelled, immobilised actin filaments (see Movie S1). Kymographs (bottom) show the diffusion of mobile actin filaments from the micrograph above along the immobilised filaments. ( D ) Time-lapse micrographs (top) and a kymograph (bottom) showing anillin-driven sliding of a mobile actin filament (bright) along an immobilised filament (dim), increasing the overlap between the two actin filaments (see Movie S2). Green and magenta arrowheads indicate the ends of the immobilised and mobile filaments, respectively. This experiment was repeated 5 times (8 events observed) with similar results. ( E ) Time-lapse micrographs showing anillin-driven sliding of two mobile actin filaments along each other, increasing the overlap between the two actin filaments (see Movie S3). Arrowheads indicate the filaments’ ends. This experiment was repeated 15 times (24 events observed) with similar results. ( F ) A typical time trace of the overlap expansion (green dots) reaching an equilibrium value (magenta line represents an exponential fit (Methods) to the data). ( G ) Velocity of the overlap expansion decreases with increasing overlap length. Green points represent an exemplary event, magenta line is a linear fit to the data.

    Article Snippet: Fluorescently-labelled microtubules were polymerized from 4 mg/ml porcine tubulin (80% unlabelled and 20% Alexa Fluor 647 NHS ester-labelled; Thermo Fisher Scientific) for 2 h at 37 °C in BRB80 (80 mM PIPES, 1 mM EGTA, 1 mM MgCl2 , pH 6.9) supplemented with 1 mM MgCl2 and 1 mM GMPCPP (Jena Bioscience, Jena, Germany).

    Techniques: Fluorescence, Diffusion-based Assay