Structured Review

Sarstedt microtube
Procedure for blood sampling in lightly restrained cynomolgus monkeys. (A) The lateral side of the heel is visualized. (B) After local disinfection and puncture with a 21-gauge needle, blood (50 µL) is collected in a <t>microtube</t> precoated with lithium
Microtube, supplied by Sarstedt, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/microtube/product/Sarstedt
Average 92 stars, based on 1 article reviews
Price from $9.99 to $1999.99
microtube - by Bioz Stars, 2020-09
92/100 stars

Images

1) Product Images from "Miniaturized Blood Sampling Techniques to Benefit Reduction in Mice and Refinement in Nonhuman Primates: Applications to Bioanalysis in Toxicity Studies with Antibody–Drug Conjugates"

Article Title: Miniaturized Blood Sampling Techniques to Benefit Reduction in Mice and Refinement in Nonhuman Primates: Applications to Bioanalysis in Toxicity Studies with Antibody–Drug Conjugates

Journal: Journal of the American Association for Laboratory Animal Science : JAALAS

doi:

Procedure for blood sampling in lightly restrained cynomolgus monkeys. (A) The lateral side of the heel is visualized. (B) After local disinfection and puncture with a 21-gauge needle, blood (50 µL) is collected in a microtube precoated with lithium
Figure Legend Snippet: Procedure for blood sampling in lightly restrained cynomolgus monkeys. (A) The lateral side of the heel is visualized. (B) After local disinfection and puncture with a 21-gauge needle, blood (50 µL) is collected in a microtube precoated with lithium

Techniques Used: Sampling

Related Articles

High Performance Liquid Chromatography:

Article Title: Synthesis of Fluorescence Turn-On DNA Hybridization Probe using the DEAtC 2’-deoxycytidine Analogue
Article Snippet: .. 5′- O -Dimethoxytrityl- N 2 -isobutyryl-2′-deoxyguanosine, 3′- O -succinyl-long chain alkylamino-CPG (LCAA-CPG) support (500 Å pore size, 43 μmol/g, Glen Research) Standard DNA phosphoramidites (dried over activated 3Å molecular sieves at least 16 hrs): N 6 -Benzoyl-2′-deoxyadenosine phosphoramidite (Bz-dA, ChemGenes) N 4 -Acetyl-2′-deoxycytidine phosphoramidite (Ac-dC, Glen Research) N 2 -Isobutyryl-2′-deoxyguanosine phosphoramidite (iBu-dG, ChemGenes) Thymidine phosphoramidite (T, Glen Research) Phosphoramidite 8 (see ) Reagents for solid-phase oligonucleotide synthesis: Activator solution: 1 H -tetrazole in anhydrous acetonitrile (Glen Research) Cap A: Acetic anhydride (Alfa Aesar):2,6-lutidine (Acros):THF (1:1:8) Cap B: 16% 1-methylimidazole (Alfa Aesar) in THF (v/v) Oxidizing solution: 0.03 M I2 (Alfa Aesar) in THF/pyridine/H2 O (75:20:2) Deblocking Mix: 3% trichloroacetic acid in CH2 Cl2 Anhydrous acetonitrile wash (CH3 CN, Glen Research) Conc. aq. ammonia (28 – 30%, Sigma-Aldrich) Acetic Acid (Fisher Scientific, HPLC grade) 3 M sodium acetate (EM Science) 5 M sodium perchlorate (GFS Chemicals, HPLC grade) Acetone (EMD, HPLC grade) Ethanol (200 Proof, Pharmco-Aaper) Mobile phase A: 0.05 M triethylammonium acetate (TEAA, Glen Research), pH 7 Mobile phase B: 25% H2 O in acetonitrile ZipTipC18 Pipette Tips (Millipore) Matrix solution for MALDI-MS: 10 mg 2,4,6-trihydroxyacetophenone (THAP, Matrix Scientific) in 1:1 MeCN:H2 O (1 mL), 230-mg ammonium citrate, dibasic (Chem Impex) in 10 mL H2 O, mix 8:1 THAP:ammonium citrate Expedite 8909 Nucleic Acid Synthesis System Empty synthesis column, 0.2 μmol scale (Glen Research) 1- and 2- mL plastic syringes 1.5 mL microtube with o-ring cap (Sarstedt) 55 °C incubation block (Eppendorf ThermoStat Plus) 1.7 mL microcentrifuge tubes (Sorenson Bioscience) Speedvac evaporator (Labconco refrigerated centrivap concentrator, Model 7310021, or equivalent) Thermo Fisher Vanquish UHPLC system equipped with: Hypersil Gold C18 column (5 μm, 4.6 × 150 mm, Thermo Fisher) Fraction Collector (Dionex UltiMate 3000) 1. ..

Amplification:

Article Title: Performance of the TruGene Human Immunodeficiency Virus Type 1 Genotyping Kit and OpenGene DNA Sequencing System on Clinical Samples Diluted to Approximately 100 Copies per Milliliter
Article Snippet: .. Viruses in the diluted sample were only when PCR amplification failed concentrated: one milliliter was centrifuged at 23,500 × g and 4°C for 75 min in a 2-ml microtube (PN 72.693.005; Sarstedt Inc., Newton, NC), and 800 μl of supernatant was removed. .. The manual extraction of 140 μl of original, diluted, or diluted-centrifuged samples was performed using the materials and Mini Spin protocol provided by the QIAamp viral RNA minikit (QIAGEN Inc., Valencia, CA) and absolute ethanol (catalog no. E7023; Sigma-Aldrich, St. Louis, MO).

Transferring:

Article Title: Synthesis of Fluorescence Turn-On DNA Hybridization Probe using the DEAtC 2’-deoxycytidine Analogue
Article Snippet: .. 5′- O -Dimethoxytrityl- N 2 -isobutyryl-2′-deoxyguanosine, 3′- O -succinyl-long chain alkylamino-CPG (LCAA-CPG) support (500 Å pore size, 43 μmol/g, Glen Research) Standard DNA phosphoramidites (dried over activated 3Å molecular sieves at least 16 hrs): N 6 -Benzoyl-2′-deoxyadenosine phosphoramidite (Bz-dA, ChemGenes) N 4 -Acetyl-2′-deoxycytidine phosphoramidite (Ac-dC, Glen Research) N 2 -Isobutyryl-2′-deoxyguanosine phosphoramidite (iBu-dG, ChemGenes) Thymidine phosphoramidite (T, Glen Research) Phosphoramidite 8 (see ) Reagents for solid-phase oligonucleotide synthesis: Activator solution: 1 H -tetrazole in anhydrous acetonitrile (Glen Research) Cap A: Acetic anhydride (Alfa Aesar):2,6-lutidine (Acros):THF (1:1:8) Cap B: 16% 1-methylimidazole (Alfa Aesar) in THF (v/v) Oxidizing solution: 0.03 M I2 (Alfa Aesar) in THF/pyridine/H2 O (75:20:2) Deblocking Mix: 3% trichloroacetic acid in CH2 Cl2 Anhydrous acetonitrile wash (CH3 CN, Glen Research) Conc. aq. ammonia (28 – 30%, Sigma-Aldrich) Acetic Acid (Fisher Scientific, HPLC grade) 3 M sodium acetate (EM Science) 5 M sodium perchlorate (GFS Chemicals, HPLC grade) Acetone (EMD, HPLC grade) Ethanol (200 Proof, Pharmco-Aaper) Mobile phase A: 0.05 M triethylammonium acetate (TEAA, Glen Research), pH 7 Mobile phase B: 25% H2 O in acetonitrile ZipTipC18 Pipette Tips (Millipore) Matrix solution for MALDI-MS: 10 mg 2,4,6-trihydroxyacetophenone (THAP, Matrix Scientific) in 1:1 MeCN:H2 O (1 mL), 230-mg ammonium citrate, dibasic (Chem Impex) in 10 mL H2 O, mix 8:1 THAP:ammonium citrate Expedite 8909 Nucleic Acid Synthesis System Empty synthesis column, 0.2 μmol scale (Glen Research) 1- and 2- mL plastic syringes 1.5 mL microtube with o-ring cap (Sarstedt) 55 °C incubation block (Eppendorf ThermoStat Plus) 1.7 mL microcentrifuge tubes (Sorenson Bioscience) Speedvac evaporator (Labconco refrigerated centrivap concentrator, Model 7310021, or equivalent) Thermo Fisher Vanquish UHPLC system equipped with: Hypersil Gold C18 column (5 μm, 4.6 × 150 mm, Thermo Fisher) Fraction Collector (Dionex UltiMate 3000) 1. ..

Blocking Assay:

Article Title: Synthesis of Fluorescence Turn-On DNA Hybridization Probe using the DEAtC 2’-deoxycytidine Analogue
Article Snippet: .. 5′- O -Dimethoxytrityl- N 2 -isobutyryl-2′-deoxyguanosine, 3′- O -succinyl-long chain alkylamino-CPG (LCAA-CPG) support (500 Å pore size, 43 μmol/g, Glen Research) Standard DNA phosphoramidites (dried over activated 3Å molecular sieves at least 16 hrs): N 6 -Benzoyl-2′-deoxyadenosine phosphoramidite (Bz-dA, ChemGenes) N 4 -Acetyl-2′-deoxycytidine phosphoramidite (Ac-dC, Glen Research) N 2 -Isobutyryl-2′-deoxyguanosine phosphoramidite (iBu-dG, ChemGenes) Thymidine phosphoramidite (T, Glen Research) Phosphoramidite 8 (see ) Reagents for solid-phase oligonucleotide synthesis: Activator solution: 1 H -tetrazole in anhydrous acetonitrile (Glen Research) Cap A: Acetic anhydride (Alfa Aesar):2,6-lutidine (Acros):THF (1:1:8) Cap B: 16% 1-methylimidazole (Alfa Aesar) in THF (v/v) Oxidizing solution: 0.03 M I2 (Alfa Aesar) in THF/pyridine/H2 O (75:20:2) Deblocking Mix: 3% trichloroacetic acid in CH2 Cl2 Anhydrous acetonitrile wash (CH3 CN, Glen Research) Conc. aq. ammonia (28 – 30%, Sigma-Aldrich) Acetic Acid (Fisher Scientific, HPLC grade) 3 M sodium acetate (EM Science) 5 M sodium perchlorate (GFS Chemicals, HPLC grade) Acetone (EMD, HPLC grade) Ethanol (200 Proof, Pharmco-Aaper) Mobile phase A: 0.05 M triethylammonium acetate (TEAA, Glen Research), pH 7 Mobile phase B: 25% H2 O in acetonitrile ZipTipC18 Pipette Tips (Millipore) Matrix solution for MALDI-MS: 10 mg 2,4,6-trihydroxyacetophenone (THAP, Matrix Scientific) in 1:1 MeCN:H2 O (1 mL), 230-mg ammonium citrate, dibasic (Chem Impex) in 10 mL H2 O, mix 8:1 THAP:ammonium citrate Expedite 8909 Nucleic Acid Synthesis System Empty synthesis column, 0.2 μmol scale (Glen Research) 1- and 2- mL plastic syringes 1.5 mL microtube with o-ring cap (Sarstedt) 55 °C incubation block (Eppendorf ThermoStat Plus) 1.7 mL microcentrifuge tubes (Sorenson Bioscience) Speedvac evaporator (Labconco refrigerated centrivap concentrator, Model 7310021, or equivalent) Thermo Fisher Vanquish UHPLC system equipped with: Hypersil Gold C18 column (5 μm, 4.6 × 150 mm, Thermo Fisher) Fraction Collector (Dionex UltiMate 3000) 1. ..

Oligonucleotide Synthesis:

Article Title: Synthesis of Fluorescence Turn-On DNA Hybridization Probe using the DEAtC 2’-deoxycytidine Analogue
Article Snippet: .. 5′- O -Dimethoxytrityl- N 2 -isobutyryl-2′-deoxyguanosine, 3′- O -succinyl-long chain alkylamino-CPG (LCAA-CPG) support (500 Å pore size, 43 μmol/g, Glen Research) Standard DNA phosphoramidites (dried over activated 3Å molecular sieves at least 16 hrs): N 6 -Benzoyl-2′-deoxyadenosine phosphoramidite (Bz-dA, ChemGenes) N 4 -Acetyl-2′-deoxycytidine phosphoramidite (Ac-dC, Glen Research) N 2 -Isobutyryl-2′-deoxyguanosine phosphoramidite (iBu-dG, ChemGenes) Thymidine phosphoramidite (T, Glen Research) Phosphoramidite 8 (see ) Reagents for solid-phase oligonucleotide synthesis: Activator solution: 1 H -tetrazole in anhydrous acetonitrile (Glen Research) Cap A: Acetic anhydride (Alfa Aesar):2,6-lutidine (Acros):THF (1:1:8) Cap B: 16% 1-methylimidazole (Alfa Aesar) in THF (v/v) Oxidizing solution: 0.03 M I2 (Alfa Aesar) in THF/pyridine/H2 O (75:20:2) Deblocking Mix: 3% trichloroacetic acid in CH2 Cl2 Anhydrous acetonitrile wash (CH3 CN, Glen Research) Conc. aq. ammonia (28 – 30%, Sigma-Aldrich) Acetic Acid (Fisher Scientific, HPLC grade) 3 M sodium acetate (EM Science) 5 M sodium perchlorate (GFS Chemicals, HPLC grade) Acetone (EMD, HPLC grade) Ethanol (200 Proof, Pharmco-Aaper) Mobile phase A: 0.05 M triethylammonium acetate (TEAA, Glen Research), pH 7 Mobile phase B: 25% H2 O in acetonitrile ZipTipC18 Pipette Tips (Millipore) Matrix solution for MALDI-MS: 10 mg 2,4,6-trihydroxyacetophenone (THAP, Matrix Scientific) in 1:1 MeCN:H2 O (1 mL), 230-mg ammonium citrate, dibasic (Chem Impex) in 10 mL H2 O, mix 8:1 THAP:ammonium citrate Expedite 8909 Nucleic Acid Synthesis System Empty synthesis column, 0.2 μmol scale (Glen Research) 1- and 2- mL plastic syringes 1.5 mL microtube with o-ring cap (Sarstedt) 55 °C incubation block (Eppendorf ThermoStat Plus) 1.7 mL microcentrifuge tubes (Sorenson Bioscience) Speedvac evaporator (Labconco refrigerated centrivap concentrator, Model 7310021, or equivalent) Thermo Fisher Vanquish UHPLC system equipped with: Hypersil Gold C18 column (5 μm, 4.6 × 150 mm, Thermo Fisher) Fraction Collector (Dionex UltiMate 3000) 1. ..

Incubation:

Article Title: Synthesis of Fluorescence Turn-On DNA Hybridization Probe using the DEAtC 2’-deoxycytidine Analogue
Article Snippet: .. 5′- O -Dimethoxytrityl- N 2 -isobutyryl-2′-deoxyguanosine, 3′- O -succinyl-long chain alkylamino-CPG (LCAA-CPG) support (500 Å pore size, 43 μmol/g, Glen Research) Standard DNA phosphoramidites (dried over activated 3Å molecular sieves at least 16 hrs): N 6 -Benzoyl-2′-deoxyadenosine phosphoramidite (Bz-dA, ChemGenes) N 4 -Acetyl-2′-deoxycytidine phosphoramidite (Ac-dC, Glen Research) N 2 -Isobutyryl-2′-deoxyguanosine phosphoramidite (iBu-dG, ChemGenes) Thymidine phosphoramidite (T, Glen Research) Phosphoramidite 8 (see ) Reagents for solid-phase oligonucleotide synthesis: Activator solution: 1 H -tetrazole in anhydrous acetonitrile (Glen Research) Cap A: Acetic anhydride (Alfa Aesar):2,6-lutidine (Acros):THF (1:1:8) Cap B: 16% 1-methylimidazole (Alfa Aesar) in THF (v/v) Oxidizing solution: 0.03 M I2 (Alfa Aesar) in THF/pyridine/H2 O (75:20:2) Deblocking Mix: 3% trichloroacetic acid in CH2 Cl2 Anhydrous acetonitrile wash (CH3 CN, Glen Research) Conc. aq. ammonia (28 – 30%, Sigma-Aldrich) Acetic Acid (Fisher Scientific, HPLC grade) 3 M sodium acetate (EM Science) 5 M sodium perchlorate (GFS Chemicals, HPLC grade) Acetone (EMD, HPLC grade) Ethanol (200 Proof, Pharmco-Aaper) Mobile phase A: 0.05 M triethylammonium acetate (TEAA, Glen Research), pH 7 Mobile phase B: 25% H2 O in acetonitrile ZipTipC18 Pipette Tips (Millipore) Matrix solution for MALDI-MS: 10 mg 2,4,6-trihydroxyacetophenone (THAP, Matrix Scientific) in 1:1 MeCN:H2 O (1 mL), 230-mg ammonium citrate, dibasic (Chem Impex) in 10 mL H2 O, mix 8:1 THAP:ammonium citrate Expedite 8909 Nucleic Acid Synthesis System Empty synthesis column, 0.2 μmol scale (Glen Research) 1- and 2- mL plastic syringes 1.5 mL microtube with o-ring cap (Sarstedt) 55 °C incubation block (Eppendorf ThermoStat Plus) 1.7 mL microcentrifuge tubes (Sorenson Bioscience) Speedvac evaporator (Labconco refrigerated centrivap concentrator, Model 7310021, or equivalent) Thermo Fisher Vanquish UHPLC system equipped with: Hypersil Gold C18 column (5 μm, 4.6 × 150 mm, Thermo Fisher) Fraction Collector (Dionex UltiMate 3000) 1. ..

other:

Article Title: Engineering of Yarrowia lipolytica for production of astaxanthin
Article Snippet: 2.5.1 Carotenoids extraction At the end of cultivation, 1 mL of the cultivation broth was transferred into a 2 mL microtube (Sarstedt) for carotenoids extraction.

Polymerase Chain Reaction:

Article Title: Performance of the TruGene Human Immunodeficiency Virus Type 1 Genotyping Kit and OpenGene DNA Sequencing System on Clinical Samples Diluted to Approximately 100 Copies per Milliliter
Article Snippet: .. Viruses in the diluted sample were only when PCR amplification failed concentrated: one milliliter was centrifuged at 23,500 × g and 4°C for 75 min in a 2-ml microtube (PN 72.693.005; Sarstedt Inc., Newton, NC), and 800 μl of supernatant was removed. .. The manual extraction of 140 μl of original, diluted, or diluted-centrifuged samples was performed using the materials and Mini Spin protocol provided by the QIAamp viral RNA minikit (QIAGEN Inc., Valencia, CA) and absolute ethanol (catalog no. E7023; Sigma-Aldrich, St. Louis, MO).

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 88
    Sarstedt polypropylene microtube
    Procedure for blood sampling in lightly restrained cynomolgus monkeys. (A) The lateral side of the heel is visualized. (B) After local disinfection and puncture with a 21-gauge needle, blood (50 µL) is collected in a <t>microtube</t> precoated with lithium
    Polypropylene Microtube, supplied by Sarstedt, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polypropylene microtube/product/Sarstedt
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    polypropylene microtube - by Bioz Stars, 2020-09
    88/100 stars
      Buy from Supplier

    92
    Sarstedt microtube
    Procedure for blood sampling in lightly restrained cynomolgus monkeys. (A) The lateral side of the heel is visualized. (B) After local disinfection and puncture with a 21-gauge needle, blood (50 µL) is collected in a <t>microtube</t> precoated with lithium
    Microtube, supplied by Sarstedt, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/microtube/product/Sarstedt
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    microtube - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    88
    Sarstedt screw cap microtube
    Procedure for blood sampling in lightly restrained cynomolgus monkeys. (A) The lateral side of the heel is visualized. (B) After local disinfection and puncture with a 21-gauge needle, blood (50 µL) is collected in a <t>microtube</t> precoated with lithium
    Screw Cap Microtube, supplied by Sarstedt, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/screw cap microtube/product/Sarstedt
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    screw cap microtube - by Bioz Stars, 2020-09
    88/100 stars
      Buy from Supplier

    Image Search Results


    Procedure for blood sampling in lightly restrained cynomolgus monkeys. (A) The lateral side of the heel is visualized. (B) After local disinfection and puncture with a 21-gauge needle, blood (50 µL) is collected in a microtube precoated with lithium

    Journal: Journal of the American Association for Laboratory Animal Science : JAALAS

    Article Title: Miniaturized Blood Sampling Techniques to Benefit Reduction in Mice and Refinement in Nonhuman Primates: Applications to Bioanalysis in Toxicity Studies with Antibody–Drug Conjugates

    doi:

    Figure Lengend Snippet: Procedure for blood sampling in lightly restrained cynomolgus monkeys. (A) The lateral side of the heel is visualized. (B) After local disinfection and puncture with a 21-gauge needle, blood (50 µL) is collected in a microtube precoated with lithium

    Article Snippet: An exact volume of 5 µL of plasma was withdrawn by using a single-channel digital electronic pipette (0.2 to 10 μL; Proline, Biohit, Dourdan, France) under magnification and transferred into a polypropylene microtube of 0.5 mL (Sarstedt) prefilled with 495 µL of a buffer solution (0.05% Tween 20 and 0.5% BSA in PBS).

    Techniques: Sampling

    Procedure for blood sampling in lightly restrained cynomolgus monkeys. (A) The lateral side of the heel is visualized. (B) After local disinfection and puncture with a 21-gauge needle, blood (50 µL) is collected in a microtube precoated with lithium

    Journal: Journal of the American Association for Laboratory Animal Science : JAALAS

    Article Title: Miniaturized Blood Sampling Techniques to Benefit Reduction in Mice and Refinement in Nonhuman Primates: Applications to Bioanalysis in Toxicity Studies with Antibody–Drug Conjugates

    doi:

    Figure Lengend Snippet: Procedure for blood sampling in lightly restrained cynomolgus monkeys. (A) The lateral side of the heel is visualized. (B) After local disinfection and puncture with a 21-gauge needle, blood (50 µL) is collected in a microtube precoated with lithium

    Article Snippet: Blood was collected in a 50-µL microtube precoated with lithium heparin (Minivette POCT, Sarstedt), flushed into a 100-µL conical microinsert, and placed in a 1.5-mL screwcap glass vial, as described earlier in part 1.

    Techniques: Sampling