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Precisionary microtome
Microtome, supplied by Precisionary, used in various techniques. Bioz Stars score: 95/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/microtome/product/Precisionary
Average 95 stars, based on 3 article reviews
Price from $9.99 to $1999.99
microtome - by Bioz Stars, 2020-04
95/100 stars

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Related Articles

Electroporation:

Article Title: Mouse Thalamic Differentiation: Gli-Dependent Pattern and Gli-Independent Prepattern
Article Snippet: Paragraph title: In utero electroporation ... The embryonic brains were dissected out, sectioned into 75 μm thick sections with a vibrating microtome (Compresstome VF-300, Precisionary Instruments Inc., Greenville, NC, USA) and observed and photographed with a Zeiss confocal microscope.

Centrifugation:

Article Title: Development of an Ex Vivo Tissue Platform To Study the Human Lung Response to Coxiella burnetii
Article Snippet: Ammonium chloride was neutralized by addition of culture medium and removed by centrifugation. .. Sections were then cored with an 8-mm-diameter tool, and tissue was sectioned into 750-μm precision-cut lung slices (PCLS) using a vibrating microtome (Compresstome; Precisionary Instruments).

Agarose Gel Electrophoresis:

Article Title: Experimental data suggesting that inflammation mediated rat liver mitochondrial dysfunction results from secondary hypoxia rather than from direct effects of inflammatory mediators
Article Snippet: Cylindrical tissue cores of 6 mm diameter were punched from the left lateral liver lobe and embedded in 3% agarose gel. .. Slices of 250 μm thickness were cut using a microtome (compresstome VF-300, Precisionary Instruments Inc., Greenville, NC, USA) at room temperature.

Plasmid Preparation:

Article Title: Axonal and subcellular labeling using modified rabies viral vectors
Article Snippet: Histology and imaging 6–7 days following vector injection, mice were perfused transcardially with 4% paraformaldehyde in phosphate-buffered saline. .. Brains were postfixed in 4% paraformaldehyde overnight at 4°C and cut into 50 um coronal sections using a vibrating microtome (VF-700; Precisionary Instruments, San Jose, CA).

Cell Culture:

Article Title: Development of an Ex Vivo Tissue Platform To Study the Human Lung Response to Coxiella burnetii
Article Snippet: Paragraph title: Bacteria and eukaryotic cell culture. ... Sections were then cored with an 8-mm-diameter tool, and tissue was sectioned into 750-μm precision-cut lung slices (PCLS) using a vibrating microtome (Compresstome; Precisionary Instruments).

Article Title: Infection of Primary Human Alveolar Macrophages Alters Staphylococcus aureus Toxin Production and Activity
Article Snippet: .. To obtain human precision cut lung slices (hPCLS), lungs were infused with low-melting-temperature agarose and allowed to solidify at 4°C. hPCLS were obtained by coring two-inch tissue sections into 8-mm-by-750-μm-diameter slices using a microtome (Compresstome; Precisionary Instruments). hPCLS were cultured in DMEM/F-12 with 10% FBS and the antibiotic/antimycotic mixture described above with shaking at 37°C and 5% CO2 . hPCLS were incubated for 3 days and then transferred to antibiotic/antimycotic-free medium for 24 h prior to infection. .. S. aureus MSSA (USA200 UAMS-1) and MRSA (USA300 LAC) strains were cultured in TSB or CCY media for 18 h until the stationary phase was reached. mCherry- and GFP-expressing S. aureus strains were constructed using methodology from Boles et al. ( ) using a plasmid constitutively expressing GFP under the control of a sarA promoter.

Article Title: Infection of Primary Human Alveolar Macrophages Alters Staphylococcus aureus Toxin Production and Activity
Article Snippet: .. To obtain human precision cut lung slices (hPCLS), lungs were infused with low-melting-temperature agarose and allowed to solidify at 4°C. hPCLS were obtained by coring two-inch tissue sections into 8-mm-by-750-μm-diameter slices using a microtome (Compresstome; Precisionary Instruments). hPCLS were cultured in DMEM/F-12 with 10% FBS and the antibiotic/antimycotic mixture described above with shaking at 37°C and 5% CO2 . hPCLS were incubated for 3 days and then transferred to antibiotic/antimycotic-free medium for 24 h prior to infection. .. S. aureus MSSA (USA200 UAMS-1) and MRSA (USA300 LAC) strains were cultured in TSB or CCY media for 18 h until the stationary phase was reached. mCherry- and GFP-expressing S. aureus strains were constructed using methodology from Boles et al. ( ) using a plasmid constitutively expressing GFP under the control of a sarA promoter.

Article Title: Characterization of Early Stages of Human Alveolar Infection by the Q Fever Agent Coxiella burnetii
Article Snippet: .. Human precision-cut lung slices (hPCLS) (diameter, 8 mm; thickness, 750 μm) were obtained using a microtome (Compresstome; Precisionary Instruments) to section cores from 1-in-thick lung slices. hPCLS were cultured at 37°C under 5% CO2 in DMEM/F12 medium containing 10% FBS, penicillin (50 U/ml), streptomycin sulfate (50 μg/ml), gentamicin sulfate (50 μg/ml), and amphotericin B (0.25 μg/ml). .. The antibiotic/antimycotic-containing medium was replaced with DMEM/F12 medium containing 10% FBS 24 h prior to infection with mCherry-expressing C. burnetii .

Article Title: Characterization of Early Stages of Human Alveolar Infection by the Q Fever Agent Coxiella burnetii
Article Snippet: .. Human precision-cut lung slices (hPCLS) (diameter, 8 mm; thickness, 750 μm) were obtained using a microtome (Compresstome; Precisionary Instruments) to section cores from 1-in-thick lung slices. hPCLS were cultured at 37°C under 5% CO2 in DMEM/F12 medium containing 10% FBS, penicillin (50 U/ml), streptomycin sulfate (50 μg/ml), gentamicin sulfate (50 μg/ml), and amphotericin B (0.25 μg/ml). .. The antibiotic/antimycotic-containing medium was replaced with DMEM/F12 medium containing 10% FBS 24 h prior to infection with mCherry-expressing C. burnetii .

Microscopy:

Article Title: Development of an Ex Vivo Tissue Platform To Study the Human Lung Response to Coxiella burnetii
Article Snippet: Cells were allowed to adhere to tissue culture dishes for 2 h, and then nonadherent cells were removed by washing, leaving a > 95% population of hAMs as confirmed by immunofluorescence microscopy (data not shown). .. Sections were then cored with an 8-mm-diameter tool, and tissue was sectioned into 750-μm precision-cut lung slices (PCLS) using a vibrating microtome (Compresstome; Precisionary Instruments).

Article Title: Mouse Thalamic Differentiation: Gli-Dependent Pattern and Gli-Independent Prepattern
Article Snippet: .. The embryonic brains were dissected out, sectioned into 75 μm thick sections with a vibrating microtome (Compresstome VF-300, Precisionary Instruments Inc., Greenville, NC, USA) and observed and photographed with a Zeiss confocal microscope. .. Statistics Statistical assessment of the electroporation data was performed with Prism 5 software (GraphPad Software, San Diego, CA, USA).

Mouse Assay:

Article Title: KCNH2-3.1 expression impairs cognition and alters neuronal function in a model of molecular pathology associated with schizophrenia
Article Snippet: Medial Prefrontal Whole-Cell Recording Control NSE-tTA (KCNH2 −/+) and KCNH2 -3.1 transgenic (KCNH2 +/+) mice at ages of postnatal days of 50–72 were used in this study. .. The brains were quickly removed after isoflurane anesthesia, and 300-μm thick coronal slices containing frontal cortex were cut on a vibrating microtome (VF-200 Microtome, Precisionary Instruments, Greenville, NC).

Article Title: Mouse Thalamic Differentiation: Gli-Dependent Pattern and Gli-Independent Prepattern
Article Snippet: In short, pregnant mice at E12.5 were anesthetized with a mixture of Halothane (Isoflurane, Baxter) and oxygen (0.5 l/min) administered with a Komesaroff Anaesthetic Machine. .. The embryonic brains were dissected out, sectioned into 75 μm thick sections with a vibrating microtome (Compresstome VF-300, Precisionary Instruments Inc., Greenville, NC, USA) and observed and photographed with a Zeiss confocal microscope.

Article Title: Axonal and subcellular labeling using modified rabies viral vectors
Article Snippet: Histology and imaging 6–7 days following vector injection, mice were perfused transcardially with 4% paraformaldehyde in phosphate-buffered saline. .. Brains were postfixed in 4% paraformaldehyde overnight at 4°C and cut into 50 um coronal sections using a vibrating microtome (VF-700; Precisionary Instruments, San Jose, CA).

Article Title: Integrated Organotypic Slice Cultures and RT-QuIC (OSCAR) Assay: Implications for Translational Discovery in Protein Misfolding Diseases
Article Snippet: .. In brief, brain slices were prepared from 9- to 12-day-old mouse pups from either wild-type (WT, C57BL/6) or transgenic prion-overexpressing or knockout mice (Tga 20 or prnp−/− , respectively) using a microtome (Compresstome™ VF-300, Precisionary Instruments). ..

Slice Preparation:

Article Title: Experimental data suggesting that inflammation mediated rat liver mitochondrial dysfunction results from secondary hypoxia rather than from direct effects of inflammatory mediators
Article Snippet: The liver was extracted and placed into ice cold custodiol solution (Koehler Chemie, Bensheim, Germany) until slice preparation. .. Slices of 250 μm thickness were cut using a microtome (compresstome VF-300, Precisionary Instruments Inc., Greenville, NC, USA) at room temperature.

Transgenic Assay:

Article Title: KCNH2-3.1 expression impairs cognition and alters neuronal function in a model of molecular pathology associated with schizophrenia
Article Snippet: Medial Prefrontal Whole-Cell Recording Control NSE-tTA (KCNH2 −/+) and KCNH2 -3.1 transgenic (KCNH2 +/+) mice at ages of postnatal days of 50–72 were used in this study. .. The brains were quickly removed after isoflurane anesthesia, and 300-μm thick coronal slices containing frontal cortex were cut on a vibrating microtome (VF-200 Microtome, Precisionary Instruments, Greenville, NC).

Article Title: Integrated Organotypic Slice Cultures and RT-QuIC (OSCAR) Assay: Implications for Translational Discovery in Protein Misfolding Diseases
Article Snippet: .. In brief, brain slices were prepared from 9- to 12-day-old mouse pups from either wild-type (WT, C57BL/6) or transgenic prion-overexpressing or knockout mice (Tga 20 or prnp−/− , respectively) using a microtome (Compresstome™ VF-300, Precisionary Instruments). ..

Incubation:

Article Title: Development of an Ex Vivo Tissue Platform To Study the Human Lung Response to Coxiella burnetii
Article Snippet: Sections were then cored with an 8-mm-diameter tool, and tissue was sectioned into 750-μm precision-cut lung slices (PCLS) using a vibrating microtome (Compresstome; Precisionary Instruments). .. PCLS were incubated with shaking in DMEM-F12 containing antibiotics and antimycotics at 37°C and 5% CO2 .

Article Title: KCNH2-3.1 expression impairs cognition and alters neuronal function in a model of molecular pathology associated with schizophrenia
Article Snippet: The brains were quickly removed after isoflurane anesthesia, and 300-μm thick coronal slices containing frontal cortex were cut on a vibrating microtome (VF-200 Microtome, Precisionary Instruments, Greenville, NC). .. The slices were initially incubated at 35°C in Ringer solution (ACSF bubbled with 95% O2 and 5% CO2) containing (in mM) 124 NaCl, 2.5 KCl, 1.25 NaH2 PO4 , 2 CaCl2 , 1 MgSO4 , 26 NaHCO3 and 10 glucose, pH 7.4, and then kept at room temperature.

Article Title: Infection of Primary Human Alveolar Macrophages Alters Staphylococcus aureus Toxin Production and Activity
Article Snippet: .. To obtain human precision cut lung slices (hPCLS), lungs were infused with low-melting-temperature agarose and allowed to solidify at 4°C. hPCLS were obtained by coring two-inch tissue sections into 8-mm-by-750-μm-diameter slices using a microtome (Compresstome; Precisionary Instruments). hPCLS were cultured in DMEM/F-12 with 10% FBS and the antibiotic/antimycotic mixture described above with shaking at 37°C and 5% CO2 . hPCLS were incubated for 3 days and then transferred to antibiotic/antimycotic-free medium for 24 h prior to infection. .. S. aureus MSSA (USA200 UAMS-1) and MRSA (USA300 LAC) strains were cultured in TSB or CCY media for 18 h until the stationary phase was reached. mCherry- and GFP-expressing S. aureus strains were constructed using methodology from Boles et al. ( ) using a plasmid constitutively expressing GFP under the control of a sarA promoter.

Article Title: Infection of Primary Human Alveolar Macrophages Alters Staphylococcus aureus Toxin Production and Activity
Article Snippet: .. To obtain human precision cut lung slices (hPCLS), lungs were infused with low-melting-temperature agarose and allowed to solidify at 4°C. hPCLS were obtained by coring two-inch tissue sections into 8-mm-by-750-μm-diameter slices using a microtome (Compresstome; Precisionary Instruments). hPCLS were cultured in DMEM/F-12 with 10% FBS and the antibiotic/antimycotic mixture described above with shaking at 37°C and 5% CO2 . hPCLS were incubated for 3 days and then transferred to antibiotic/antimycotic-free medium for 24 h prior to infection. .. S. aureus MSSA (USA200 UAMS-1) and MRSA (USA300 LAC) strains were cultured in TSB or CCY media for 18 h until the stationary phase was reached. mCherry- and GFP-expressing S. aureus strains were constructed using methodology from Boles et al. ( ) using a plasmid constitutively expressing GFP under the control of a sarA promoter.

Article Title: Characterization of Early Stages of Human Alveolar Infection by the Q Fever Agent Coxiella burnetii
Article Snippet: Low-melting-temperature agarose was instilled into postmortem human lungs, which were then incubated at 4°C for solidification. .. Human precision-cut lung slices (hPCLS) (diameter, 8 mm; thickness, 750 μm) were obtained using a microtome (Compresstome; Precisionary Instruments) to section cores from 1-in-thick lung slices. hPCLS were cultured at 37°C under 5% CO2 in DMEM/F12 medium containing 10% FBS, penicillin (50 U/ml), streptomycin sulfate (50 μg/ml), gentamicin sulfate (50 μg/ml), and amphotericin B (0.25 μg/ml).

Article Title: Characterization of Early Stages of Human Alveolar Infection by the Q Fever Agent Coxiella burnetii
Article Snippet: Low-melting-temperature agarose was instilled into postmortem human lungs, which were then incubated at 4°C for solidification. .. Human precision-cut lung slices (hPCLS) (diameter, 8 mm; thickness, 750 μm) were obtained using a microtome (Compresstome; Precisionary Instruments) to section cores from 1-in-thick lung slices. hPCLS were cultured at 37°C under 5% CO2 in DMEM/F12 medium containing 10% FBS, penicillin (50 U/ml), streptomycin sulfate (50 μg/ml), gentamicin sulfate (50 μg/ml), and amphotericin B (0.25 μg/ml).

Article Title: Experimental data suggesting that inflammation mediated rat liver mitochondrial dysfunction results from secondary hypoxia rather than from direct effects of inflammatory mediators
Article Snippet: Slices of 250 μm thickness were cut using a microtome (compresstome VF-300, Precisionary Instruments Inc., Greenville, NC, USA) at room temperature. .. Slices were incubated in ice cold Ringer solution (Fresenius Kabi, Graz, Austria) for 25 min in order to wash out intracellular compounds released due to the preparation procedure.

Infection:

Article Title: Development of an Ex Vivo Tissue Platform To Study the Human Lung Response to Coxiella burnetii
Article Snippet: Sections were then cored with an 8-mm-diameter tool, and tissue was sectioned into 750-μm precision-cut lung slices (PCLS) using a vibrating microtome (Compresstome; Precisionary Instruments). .. PCLS and hAMs were cultured in antibiotic- and antimycotic-containing medium for 3 days, and then antibiotic- and antimycotic-free medium was added and samples incubated for an additional 24 h before infection to ensure absence of contamination.

Article Title: Infection of Primary Human Alveolar Macrophages Alters Staphylococcus aureus Toxin Production and Activity
Article Snippet: .. To obtain human precision cut lung slices (hPCLS), lungs were infused with low-melting-temperature agarose and allowed to solidify at 4°C. hPCLS were obtained by coring two-inch tissue sections into 8-mm-by-750-μm-diameter slices using a microtome (Compresstome; Precisionary Instruments). hPCLS were cultured in DMEM/F-12 with 10% FBS and the antibiotic/antimycotic mixture described above with shaking at 37°C and 5% CO2 . hPCLS were incubated for 3 days and then transferred to antibiotic/antimycotic-free medium for 24 h prior to infection. .. S. aureus MSSA (USA200 UAMS-1) and MRSA (USA300 LAC) strains were cultured in TSB or CCY media for 18 h until the stationary phase was reached. mCherry- and GFP-expressing S. aureus strains were constructed using methodology from Boles et al. ( ) using a plasmid constitutively expressing GFP under the control of a sarA promoter.

Article Title: Infection of Primary Human Alveolar Macrophages Alters Staphylococcus aureus Toxin Production and Activity
Article Snippet: .. To obtain human precision cut lung slices (hPCLS), lungs were infused with low-melting-temperature agarose and allowed to solidify at 4°C. hPCLS were obtained by coring two-inch tissue sections into 8-mm-by-750-μm-diameter slices using a microtome (Compresstome; Precisionary Instruments). hPCLS were cultured in DMEM/F-12 with 10% FBS and the antibiotic/antimycotic mixture described above with shaking at 37°C and 5% CO2 . hPCLS were incubated for 3 days and then transferred to antibiotic/antimycotic-free medium for 24 h prior to infection. .. S. aureus MSSA (USA200 UAMS-1) and MRSA (USA300 LAC) strains were cultured in TSB or CCY media for 18 h until the stationary phase was reached. mCherry- and GFP-expressing S. aureus strains were constructed using methodology from Boles et al. ( ) using a plasmid constitutively expressing GFP under the control of a sarA promoter.

Article Title: Characterization of Early Stages of Human Alveolar Infection by the Q Fever Agent Coxiella burnetii
Article Snippet: Human precision-cut lung slices (hPCLS) (diameter, 8 mm; thickness, 750 μm) were obtained using a microtome (Compresstome; Precisionary Instruments) to section cores from 1-in-thick lung slices. hPCLS were cultured at 37°C under 5% CO2 in DMEM/F12 medium containing 10% FBS, penicillin (50 U/ml), streptomycin sulfate (50 μg/ml), gentamicin sulfate (50 μg/ml), and amphotericin B (0.25 μg/ml). .. The antibiotic/antimycotic-containing medium was replaced with DMEM/F12 medium containing 10% FBS 24 h prior to infection with mCherry-expressing C. burnetii .

Article Title: Characterization of Early Stages of Human Alveolar Infection by the Q Fever Agent Coxiella burnetii
Article Snippet: Human precision-cut lung slices (hPCLS) (diameter, 8 mm; thickness, 750 μm) were obtained using a microtome (Compresstome; Precisionary Instruments) to section cores from 1-in-thick lung slices. hPCLS were cultured at 37°C under 5% CO2 in DMEM/F12 medium containing 10% FBS, penicillin (50 U/ml), streptomycin sulfate (50 μg/ml), gentamicin sulfate (50 μg/ml), and amphotericin B (0.25 μg/ml). .. The antibiotic/antimycotic-containing medium was replaced with DMEM/F12 medium containing 10% FBS 24 h prior to infection with mCherry-expressing C. burnetii .

In Utero:

Article Title: Mouse Thalamic Differentiation: Gli-Dependent Pattern and Gli-Independent Prepattern
Article Snippet: Paragraph title: In utero electroporation ... The embryonic brains were dissected out, sectioned into 75 μm thick sections with a vibrating microtome (Compresstome VF-300, Precisionary Instruments Inc., Greenville, NC, USA) and observed and photographed with a Zeiss confocal microscope.

Knock-Out:

Article Title: Integrated Organotypic Slice Cultures and RT-QuIC (OSCAR) Assay: Implications for Translational Discovery in Protein Misfolding Diseases
Article Snippet: .. In brief, brain slices were prepared from 9- to 12-day-old mouse pups from either wild-type (WT, C57BL/6) or transgenic prion-overexpressing or knockout mice (Tga 20 or prnp−/− , respectively) using a microtome (Compresstome™ VF-300, Precisionary Instruments). ..

Mass Spectrometry:

Article Title: Mouse Thalamic Differentiation: Gli-Dependent Pattern and Gli-Independent Prepattern
Article Snippet: Electric pulses were administered with a CUY21 electroporator (Nepagene, Japan; 5 square-wave pulses, 50 V, 50 ms on/950 ms off) and 5 mm tweezer electrodes. .. The embryonic brains were dissected out, sectioned into 75 μm thick sections with a vibrating microtome (Compresstome VF-300, Precisionary Instruments Inc., Greenville, NC, USA) and observed and photographed with a Zeiss confocal microscope.

Modification:

Article Title: Development of an Ex Vivo Tissue Platform To Study the Human Lung Response to Coxiella burnetii
Article Snippet: Cells were resuspended in Dulbecco's modified Eagle's medium-F12 (DMEM-F12) containing 10% fetal bovine serum (FBS), penicillin (50 U/ml), streptomycin sulfate (50 μg/ml), gentamicin sulfate (50 μg/ml), and amphotericin B (0.25 μg/ml) (Invitrogen). .. Sections were then cored with an 8-mm-diameter tool, and tissue was sectioned into 750-μm precision-cut lung slices (PCLS) using a vibrating microtome (Compresstome; Precisionary Instruments).

Article Title: Infection of Primary Human Alveolar Macrophages Alters Staphylococcus aureus Toxin Production and Activity
Article Snippet: BAL fluid was centrifuged, and red blood cells were lysed with 0.86% ammonium chloride at 10-min intervals and then neutralized with culture medium (Dulbecco’s modified Eagle’s medium F-12; DMEM/F-12) containing 10% fetal bovine serum (FBS), penicillin (50 U/ml), streptomycin sulfate (50 μg/ml), gentamicin sulfate (50 μg/ml), and amphotericin B (0.25 μg/ml) (Invitrogen). .. To obtain human precision cut lung slices (hPCLS), lungs were infused with low-melting-temperature agarose and allowed to solidify at 4°C. hPCLS were obtained by coring two-inch tissue sections into 8-mm-by-750-μm-diameter slices using a microtome (Compresstome; Precisionary Instruments). hPCLS were cultured in DMEM/F-12 with 10% FBS and the antibiotic/antimycotic mixture described above with shaking at 37°C and 5% CO2 . hPCLS were incubated for 3 days and then transferred to antibiotic/antimycotic-free medium for 24 h prior to infection.

Article Title: Infection of Primary Human Alveolar Macrophages Alters Staphylococcus aureus Toxin Production and Activity
Article Snippet: BAL fluid was centrifuged, and red blood cells were lysed with 0.86% ammonium chloride at 10-min intervals and then neutralized with culture medium (Dulbecco’s modified Eagle’s medium F-12; DMEM/F-12) containing 10% fetal bovine serum (FBS), penicillin (50 U/ml), streptomycin sulfate (50 μg/ml), gentamicin sulfate (50 μg/ml), and amphotericin B (0.25 μg/ml) (Invitrogen). .. To obtain human precision cut lung slices (hPCLS), lungs were infused with low-melting-temperature agarose and allowed to solidify at 4°C. hPCLS were obtained by coring two-inch tissue sections into 8-mm-by-750-μm-diameter slices using a microtome (Compresstome; Precisionary Instruments). hPCLS were cultured in DMEM/F-12 with 10% FBS and the antibiotic/antimycotic mixture described above with shaking at 37°C and 5% CO2 . hPCLS were incubated for 3 days and then transferred to antibiotic/antimycotic-free medium for 24 h prior to infection.

Injection:

Article Title: Mouse Thalamic Differentiation: Gli-Dependent Pattern and Gli-Independent Prepattern
Article Snippet: The uterus was exposed through laparotomy and the DNA solution (1 μg/μl) was injected with a glass micropipette in the ventricular system of the brain of each embryo through the uterine wall. .. The embryonic brains were dissected out, sectioned into 75 μm thick sections with a vibrating microtome (Compresstome VF-300, Precisionary Instruments Inc., Greenville, NC, USA) and observed and photographed with a Zeiss confocal microscope.

Article Title: Axonal and subcellular labeling using modified rabies viral vectors
Article Snippet: Histology and imaging 6–7 days following vector injection, mice were perfused transcardially with 4% paraformaldehyde in phosphate-buffered saline. .. Brains were postfixed in 4% paraformaldehyde overnight at 4°C and cut into 50 um coronal sections using a vibrating microtome (VF-700; Precisionary Instruments, San Jose, CA).

Immunofluorescence:

Article Title: Development of an Ex Vivo Tissue Platform To Study the Human Lung Response to Coxiella burnetii
Article Snippet: Cells were allowed to adhere to tissue culture dishes for 2 h, and then nonadherent cells were removed by washing, leaving a > 95% population of hAMs as confirmed by immunofluorescence microscopy (data not shown). .. Sections were then cored with an 8-mm-diameter tool, and tissue was sectioned into 750-μm precision-cut lung slices (PCLS) using a vibrating microtome (Compresstome; Precisionary Instruments).

Blocking Assay:

Article Title: Integrated Organotypic Slice Cultures and RT-QuIC (OSCAR) Assay: Implications for Translational Discovery in Protein Misfolding Diseases
Article Snippet: In brief, brain slices were prepared from 9- to 12-day-old mouse pups from either wild-type (WT, C57BL/6) or transgenic prion-overexpressing or knockout mice (Tga 20 or prnp−/− , respectively) using a microtome (Compresstome™ VF-300, Precisionary Instruments). .. The agar was quickly solidified by clasping the specimen tube with a chilling block, and then the specimen tube was inserted into the slicing reservoir filled with freshly prepared, ice-cold Gey’s balanced salt solution supplemented with the excitotoxic antagonist, kynurenic acid (GBSSK).

Imaging:

Article Title: Axonal and subcellular labeling using modified rabies viral vectors
Article Snippet: Paragraph title: Histology and imaging ... Brains were postfixed in 4% paraformaldehyde overnight at 4°C and cut into 50 um coronal sections using a vibrating microtome (VF-700; Precisionary Instruments, San Jose, CA).

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    Precisionary microtome
    Microtome, supplied by Precisionary, used in various techniques. Bioz Stars score: 95/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 95 stars, based on 3 article reviews
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    Precisionary compresstome vf 700 microtome sectioning
    Compresstome Vf 700 Microtome Sectioning, supplied by Precisionary, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Precisionary compresstome vf 300 vibrating microtome
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