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Olympus microtome
Microtome, supplied by Olympus, used in various techniques. Bioz Stars score: 98/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 98 stars, based on 3 article reviews
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microtome - by Bioz Stars, 2020-03
98/100 stars

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Incubation:

Article Title: Heritable endogenous gene regulation in plants with designed polydactyl zinc finger transcription factors
Article Snippet: Freshly excised floral tissues were immersed immediately in GUS staining solution containing 0.25 mM 5-bromo-4-chloro-3-indolyl β-D-glucuronide cyclohexylammonium salt (X-glu, Rose Scientific, Edmonton, AB, Canada) in 50 mM sodium phosphate buffer (pH 7.3), and then incubated 20 h at 37°C. .. Stained tissues were dehydrated in an ethanol series and photographed before infiltration with Histo-Clear (International Diagnostics, Atlanta), and paraffin-cast specimen blocks ( ) were sectioned on a microtome (Microm HM315, Mikron) and stained in situ with 0.1% aqueous Safranin-O for 5–60 s. Stained surfaces of specimen blocks were treated with immersion oil and visualized under a dissecting microscope (Olympus, New Hyde Park, NY).

Article Title: Dopamine neuron glutamate cotransmission evokes a delayed excitation in lateral dorsal striatal cholinergic interneurons
Article Snippet: .. Coronal sections of the striatum were cut, 300 µm thick, with a vibrating microtome (VT1200S, Leica), incubated in high glucose ACSF at room temperature for at least 1 hr for recovery, then transferred to the recording chamber (submerged, 500 µl volume) on the stage of an upright microscope (BX61WI, Olympus), continuously perfused with standard ACSF (in mM: 125 NaCl, 2.5 KCl, 25 NaHCO3 , 1.25 NaH2 PO4 , 2 CaCl2 , 1 MgCl2 and 25 glucose, pH 7.4) saturated with carbogen. .. ChR2-EYFP, D2-EGFP or ChAT-eGFP expression was confirmed by field illumination with a 470 nm LED; D1-tdTomato expression was confirmed with 530 nm LED illumination (DC4100, Thorlabs).

Article Title: Apolipoprotein AIV Requires Cholecystokinin and Vagal Nerves to Suppress Food Intake
Article Snippet: The frozen brain was sliced with a microtome (cut 4055; Olympus, Tokyo, Japan) and brain sections (30 μm) were stained with diluted c-Fos Ab-5 (1:30,000; Calbiochem, Gibbstown, NJ) and biotinylated goat antirabbit secondary antibody (dilution 1:200) in normal goat serum plus 0.3% Triton X-100 buffer. .. The sections were incubated with horseradish peroxidase avidin-biotin complex (dilution 1:200) and developed by diaminobenzidine with nickel sulfate.

Article Title: A Drosophila Protein Family Implicated in Pheromone Perception Is Related to Tay-Sachs GM2-Activator Protein *
Article Snippet: Samples were sectioned on a microtome (Olympus Cut 4060), collected on Superfrost plus microslides (Fisher Scientific), and dried at 39 °C overnight. .. Slides were incubated for 4 h at room temperature with guinea pig anti-CheB42a antibody ( ) at a 1:200 dilution, and when applicable, rat anti-PBPRP2 ( ) at a 1:300 dilution for 4 h at room temperature, followed by three washes in PBT.

Expressing:

Article Title: Heritable endogenous gene regulation in plants with designed polydactyl zinc finger transcription factors
Article Snippet: Paragraph title: GUS Histochemical Staining and Analysis of Expression Pattern. ... Stained tissues were dehydrated in an ethanol series and photographed before infiltration with Histo-Clear (International Diagnostics, Atlanta), and paraffin-cast specimen blocks ( ) were sectioned on a microtome (Microm HM315, Mikron) and stained in situ with 0.1% aqueous Safranin-O for 5–60 s. Stained surfaces of specimen blocks were treated with immersion oil and visualized under a dissecting microscope (Olympus, New Hyde Park, NY).

Article Title: Dopamine neuron glutamate cotransmission evokes a delayed excitation in lateral dorsal striatal cholinergic interneurons
Article Snippet: Coronal sections of the striatum were cut, 300 µm thick, with a vibrating microtome (VT1200S, Leica), incubated in high glucose ACSF at room temperature for at least 1 hr for recovery, then transferred to the recording chamber (submerged, 500 µl volume) on the stage of an upright microscope (BX61WI, Olympus), continuously perfused with standard ACSF (in mM: 125 NaCl, 2.5 KCl, 25 NaHCO3 , 1.25 NaH2 PO4 , 2 CaCl2 , 1 MgCl2 and 25 glucose, pH 7.4) saturated with carbogen. .. ChR2-EYFP, D2-EGFP or ChAT-eGFP expression was confirmed by field illumination with a 470 nm LED; D1-tdTomato expression was confirmed with 530 nm LED illumination (DC4100, Thorlabs).

Modification:

Article Title: A Drosophila Protein Family Implicated in Pheromone Perception Is Related to Tay-Sachs GM2-Activator Protein *
Article Snippet: Immunochemistry —Our immunohistochemistry protocol was modified from Ref. . .. Samples were sectioned on a microtome (Olympus Cut 4060), collected on Superfrost plus microslides (Fisher Scientific), and dried at 39 °C overnight.

Immunohistochemistry:

Article Title: Accumulation of Amyloid Precursor Protein in the Mitochondrial Import Channels of Human Alzheimer’s Disease Brain Is Associated with Mitochondrial Dysfunction
Article Snippet: Paragraph title: Triple immunohistochemistry. ... Paraffin-embedded tissues were cut into 10 μm serial sections using a microtome (Olympus Optical).

Article Title: A Drosophila Protein Family Implicated in Pheromone Perception Is Related to Tay-Sachs GM2-Activator Protein *
Article Snippet: Immunochemistry —Our immunohistochemistry protocol was modified from Ref. . .. Samples were sectioned on a microtome (Olympus Cut 4060), collected on Superfrost plus microslides (Fisher Scientific), and dried at 39 °C overnight.

Article Title: Unsaturated Fatty Acids Revert Diet-Induced Hypothalamic Inflammation in Obesity
Article Snippet: Paragraph title: Immunohistochemistry and histology ... After inclusion, 4 mm sections were obtained on a microtome (CUT model 445, Olympus).

Activation Assay:

Article Title: Apolipoprotein AIV Requires Cholecystokinin and Vagal Nerves to Suppress Food Intake
Article Snippet: Paragraph title: Neuronal activation in the hindbrain ... The frozen brain was sliced with a microtome (cut 4055; Olympus, Tokyo, Japan) and brain sections (30 μm) were stained with diluted c-Fos Ab-5 (1:30,000; Calbiochem, Gibbstown, NJ) and biotinylated goat antirabbit secondary antibody (dilution 1:200) in normal goat serum plus 0.3% Triton X-100 buffer.

Light Microscopy:

Article Title: Study of hepatotoxicity and oxidative stress in male Swiss-Webster mice exposed to functionalized multi-walled carbon nanotubes
Article Snippet: The liver specimens were processed and embedded in paraffin, sectioned at 0.1 μm using microtome (Olympus CUT 4055E, USA) and stained with hematoxylin and eosin (H & E). .. According to the method described by [ ] the morphology of liver was evaluated at 1000× magnification using Axiovert S 100, inverted light microscope (Carl Zeiss Micro Imaging Inc, Thornwood, NY, USA).

Article Title: Liposomal paclitaxel induces fewer hematopoietic and cardiovascular complications than bioequivalent doses of Taxol
Article Snippet: Sections (size, 4 µ m) were cut with a microtome (Olympus Cut 4055; Olympus Corporation, Tokyo, Japan) and mounted on slides. .. Finally, the stained sections were dehydrated and mounted with Permount (Thermo Fisher Scientific, Inc.), and examined by light microscopy.

Article Title: Effects of limited access dressing in chronic wounds: A biochemical and histological study
Article Snippet: .. Serial sections of 5-μm thickness were cut using microtome (Leica RM2255), stained with haematoxylin-eosin[ ] and examined under light microscopy (Olympus PM20). .. Statistical analysis Statistical analysis was performed using Student's t -test using the SPSS software 15th version package.

Article Title: Progesterone Induces Mucosal Immunity in a Rodent Model of Human Taeniosis by Taenia solium
Article Snippet: Once the blocks were solidified after 24 h at room temperature, duodenum tissue was cross-sectioned in thin 4 μM slices, by using a microtome (Microtome Olympus Cut 4060, USA). .. Evaluation of inflammatory infiltrate degree on H & E-stained duodenum sections was performed under a light microscope using 400 X magnification.

Article Title: Liposomal paclitaxel induces fewer hematopoietic and cardiovascular complications than bioequivalent doses of Taxol
Article Snippet: Sections (size, 4 µ m) were cut with a microtome (Olympus Cut 4055; Olympus Corporation, Tokyo, Japan) and mounted on slides. .. Finally, the stained sections were dehydrated and mounted with Permount (Thermo Fisher Scientific, Inc.), and examined by light microscopy.

Imaging:

Article Title: Study of hepatotoxicity and oxidative stress in male Swiss-Webster mice exposed to functionalized multi-walled carbon nanotubes
Article Snippet: The liver specimens were processed and embedded in paraffin, sectioned at 0.1 μm using microtome (Olympus CUT 4055E, USA) and stained with hematoxylin and eosin (H & E). .. According to the method described by [ ] the morphology of liver was evaluated at 1000× magnification using Axiovert S 100, inverted light microscope (Carl Zeiss Micro Imaging Inc, Thornwood, NY, USA).

Article Title: Preparation and Evaluation of Hot-Melt Extruded Patient-Centric Ketoprofen Mini-Tablets
Article Snippet: .. The chemical imaging samples were embedded in melted paraffin (Paraplast X-TRA® , Sigma-Aldrich, St. Louis, MO, USA) and reduced to 5-μm thickness using a microtome (Olympus America Inc., Center Valley, PA, USA) to allow IR light to penetrate it and reflect out. .. Chemical imaging was utilized to visually approximate drug homogeneity and interactions.

Injection:

Article Title: Dopamine neuron glutamate cotransmission evokes a delayed excitation in lateral dorsal striatal cholinergic interneurons
Article Snippet: Coronal sections of the striatum were cut, 300 µm thick, with a vibrating microtome (VT1200S, Leica), incubated in high glucose ACSF at room temperature for at least 1 hr for recovery, then transferred to the recording chamber (submerged, 500 µl volume) on the stage of an upright microscope (BX61WI, Olympus), continuously perfused with standard ACSF (in mM: 125 NaCl, 2.5 KCl, 25 NaHCO3 , 1.25 NaH2 PO4 , 2 CaCl2 , 1 MgCl2 and 25 glucose, pH 7.4) saturated with carbogen. .. In DATIREScre :Ai32 mice, ChIs were identified visually by large soma size, confirmed by spontaneous firing, shallow resting membrane potentials (around −60 mV) and voltage sag by −400 pA current injection (700 msec duration) ( ).

Article Title: Apolipoprotein AIV Requires Cholecystokinin and Vagal Nerves to Suppress Food Intake
Article Snippet: In the SDA study, SDA and SHAM rats (three to four rats per group) were fasted for 17 h and killed after an ip injection of either saline (0.2 ml) or rat recombinant apo AIV at 100 μg/kg in 0.2 ml saline. .. The frozen brain was sliced with a microtome (cut 4055; Olympus, Tokyo, Japan) and brain sections (30 μm) were stained with diluted c-Fos Ab-5 (1:30,000; Calbiochem, Gibbstown, NJ) and biotinylated goat antirabbit secondary antibody (dilution 1:200) in normal goat serum plus 0.3% Triton X-100 buffer.

Article Title: Liposomal paclitaxel induces fewer hematopoietic and cardiovascular complications than bioequivalent doses of Taxol
Article Snippet: For blood biochemistry examination, 24 h after the last injection, whole blood was collected in BD Microtainer® tubes (BD Biosciences) and was centrifuged at 3,000 × g for 10 min at 4°C to obtain plasma samples. .. Sections (size, 4 µ m) were cut with a microtome (Olympus Cut 4055; Olympus Corporation, Tokyo, Japan) and mounted on slides.

Article Title: Liposomal paclitaxel induces fewer hematopoietic and cardiovascular complications than bioequivalent doses of Taxol
Article Snippet: For blood biochemistry examination, 24 h after the last injection, whole blood was collected in BD Microtainer® tubes (BD Biosciences) and was centrifuged at 3,000 × g for 10 min at 4°C to obtain plasma samples. .. Sections (size, 4 µ m) were cut with a microtome (Olympus Cut 4055; Olympus Corporation, Tokyo, Japan) and mounted on slides.

Recombinant:

Article Title: Apolipoprotein AIV Requires Cholecystokinin and Vagal Nerves to Suppress Food Intake
Article Snippet: In the SDA study, SDA and SHAM rats (three to four rats per group) were fasted for 17 h and killed after an ip injection of either saline (0.2 ml) or rat recombinant apo AIV at 100 μg/kg in 0.2 ml saline. .. The frozen brain was sliced with a microtome (cut 4055; Olympus, Tokyo, Japan) and brain sections (30 μm) were stained with diluted c-Fos Ab-5 (1:30,000; Calbiochem, Gibbstown, NJ) and biotinylated goat antirabbit secondary antibody (dilution 1:200) in normal goat serum plus 0.3% Triton X-100 buffer.

Histopathology:

Article Title: Study of hepatotoxicity and oxidative stress in male Swiss-Webster mice exposed to functionalized multi-walled carbon nanotubes
Article Snippet: Paragraph title: Histopathology Evaluation of tissue ... The liver specimens were processed and embedded in paraffin, sectioned at 0.1 μm using microtome (Olympus CUT 4055E, USA) and stained with hematoxylin and eosin (H & E).

In Vivo:

Article Title: Liposomal paclitaxel induces fewer hematopoietic and cardiovascular complications than bioequivalent doses of Taxol
Article Snippet: Paragraph title: In vivo toxicity assay ... Sections (size, 4 µ m) were cut with a microtome (Olympus Cut 4055; Olympus Corporation, Tokyo, Japan) and mounted on slides.

Fluorescence:

Article Title: Stable Liposome in Cosmetic Platforms for Transdermal Folic acid delivery for fortification and treatment of micronutrient deficiencies
Article Snippet: .. Transverse sections of the treated area of skin were sliced using microtome (~10 µm thickness) to generate XZ-planar optical cross-sections and scanned under a confocal laser scanning microscope (Olympus IX 81 with FV 500) for fluorescence signals with an argon laser beam with excitation at 488 nm and emission at 560 nm for Rhodamine-B . ..

AST Assay:

Article Title: Liposomal paclitaxel induces fewer hematopoietic and cardiovascular complications than bioequivalent doses of Taxol
Article Snippet: Plasma samples were supplied to Taiwan Mouse Clinic to determine blood Ca2+ concentration, alanine aminotransferase (ALT), aspartate aminotransferase (AST), total bilirubin (TBIL), blood urea nitrogen (BUN) and uric acid (UA) using the Fuji Dri-Chem 4000i (Fujifilm, Tokyo, Japan). .. Sections (size, 4 µ m) were cut with a microtome (Olympus Cut 4055; Olympus Corporation, Tokyo, Japan) and mounted on slides.

Article Title: Liposomal paclitaxel induces fewer hematopoietic and cardiovascular complications than bioequivalent doses of Taxol
Article Snippet: Plasma samples were supplied to Taiwan Mouse Clinic to determine blood Ca2+ concentration, alanine aminotransferase (ALT), aspartate aminotransferase (AST), total bilirubin (TBIL), blood urea nitrogen (BUN) and uric acid (UA) using the Fuji Dri-Chem 4000i (Fujifilm, Tokyo, Japan). .. Sections (size, 4 µ m) were cut with a microtome (Olympus Cut 4055; Olympus Corporation, Tokyo, Japan) and mounted on slides.

Avidin-Biotin Assay:

Article Title: Apolipoprotein AIV Requires Cholecystokinin and Vagal Nerves to Suppress Food Intake
Article Snippet: The frozen brain was sliced with a microtome (cut 4055; Olympus, Tokyo, Japan) and brain sections (30 μm) were stained with diluted c-Fos Ab-5 (1:30,000; Calbiochem, Gibbstown, NJ) and biotinylated goat antirabbit secondary antibody (dilution 1:200) in normal goat serum plus 0.3% Triton X-100 buffer. .. The sections were incubated with horseradish peroxidase avidin-biotin complex (dilution 1:200) and developed by diaminobenzidine with nickel sulfate.

Microscopy:

Article Title: Study of hepatotoxicity and oxidative stress in male Swiss-Webster mice exposed to functionalized multi-walled carbon nanotubes
Article Snippet: The liver specimens were processed and embedded in paraffin, sectioned at 0.1 μm using microtome (Olympus CUT 4055E, USA) and stained with hematoxylin and eosin (H & E). .. The stained tissues were visualized under the microscope and evaluated for the changes in morphology of MWCNTs exposed liver compared with the control.

Article Title: Heritable endogenous gene regulation in plants with designed polydactyl zinc finger transcription factors
Article Snippet: .. Stained tissues were dehydrated in an ethanol series and photographed before infiltration with Histo-Clear (International Diagnostics, Atlanta), and paraffin-cast specimen blocks ( ) were sectioned on a microtome (Microm HM315, Mikron) and stained in situ with 0.1% aqueous Safranin-O for 5–60 s. Stained surfaces of specimen blocks were treated with immersion oil and visualized under a dissecting microscope (Olympus, New Hyde Park, NY). .. The target sequence of the designed AP3 -specific ZFP AP3 is an 18-bp sequence located in the AP3 promoter region, at −112 to −95 relative to the ATG codon (Fig. A ).

Article Title: Dopamine neuron glutamate cotransmission evokes a delayed excitation in lateral dorsal striatal cholinergic interneurons
Article Snippet: .. Coronal sections of the striatum were cut, 300 µm thick, with a vibrating microtome (VT1200S, Leica), incubated in high glucose ACSF at room temperature for at least 1 hr for recovery, then transferred to the recording chamber (submerged, 500 µl volume) on the stage of an upright microscope (BX61WI, Olympus), continuously perfused with standard ACSF (in mM: 125 NaCl, 2.5 KCl, 25 NaHCO3 , 1.25 NaH2 PO4 , 2 CaCl2 , 1 MgCl2 and 25 glucose, pH 7.4) saturated with carbogen. .. ChR2-EYFP, D2-EGFP or ChAT-eGFP expression was confirmed by field illumination with a 470 nm LED; D1-tdTomato expression was confirmed with 530 nm LED illumination (DC4100, Thorlabs).

Article Title: Tamoxifen Treatment in Hamsters Induces Protection during Taeniosis by Taenia solium
Article Snippet: After this time, all of the tissues were embedded in paraffin for being posterior cross-sectioned in thin 4 μ M slices, by using a microtome (Microtome Olympus Cut 4060, USA). .. Sections were stained with hematoxylin-eosin for evaluating the inflammatory infiltrate degree on each sample, considered as number of polymorphonuclear leukocytes per ten microvilli, using an optical microscope at 40 and 100x magnification (Nikon Microphot-FXA Microscope).

Article Title: Preparation and Evaluation of Hot-Melt Extruded Patient-Centric Ketoprofen Mini-Tablets
Article Snippet: Chemical imaging was conducted using Cary 620 FTIR Microscope (Agilent Technologies, Santa Clara, CA, USA) equipped with a 64 × 64 pixel focal plane array (FPA) with and without a germanium micro-ATR. .. The chemical imaging samples were embedded in melted paraffin (Paraplast X-TRA® , Sigma-Aldrich, St. Louis, MO, USA) and reduced to 5-μm thickness using a microtome (Olympus America Inc., Center Valley, PA, USA) to allow IR light to penetrate it and reflect out.

Mouse Assay:

Article Title: Dopamine neuron glutamate cotransmission evokes a delayed excitation in lateral dorsal striatal cholinergic interneurons
Article Snippet: After confirmation of full anesthesia, mice were decapitated and brains quickly removed in ice-cold high-glucose artificial cerebrospinal fluid (ACSF) (in mM: 75 NaCl, 2.5 KCl, 26 NaHCO3 , 1.25 NaH2 PO4 , 0.7 CaCl2 , 2 MgCl2 and 100 glucose, pH 7.4) saturated with mixture of 95% O2% and 5% CO2 (carbogen). .. Coronal sections of the striatum were cut, 300 µm thick, with a vibrating microtome (VT1200S, Leica), incubated in high glucose ACSF at room temperature for at least 1 hr for recovery, then transferred to the recording chamber (submerged, 500 µl volume) on the stage of an upright microscope (BX61WI, Olympus), continuously perfused with standard ACSF (in mM: 125 NaCl, 2.5 KCl, 25 NaHCO3 , 1.25 NaH2 PO4 , 2 CaCl2 , 1 MgCl2 and 25 glucose, pH 7.4) saturated with carbogen.

Article Title: Apolipoprotein AIV Requires Cholecystokinin and Vagal Nerves to Suppress Food Intake
Article Snippet: In the CCK1R study, 17 h-fasted WT mice (four mice per group) received two ip injections of saline (0.1 ml) + saline (0.1 ml), saline (0.1 ml) + apo AIV (200 μg/kg in 0.1 ml saline), or lorglumide (0.3 mg/kg in 0.1 ml saline) + AIV (200 μg/kg in 0.1 ml saline). .. The frozen brain was sliced with a microtome (cut 4055; Olympus, Tokyo, Japan) and brain sections (30 μm) were stained with diluted c-Fos Ab-5 (1:30,000; Calbiochem, Gibbstown, NJ) and biotinylated goat antirabbit secondary antibody (dilution 1:200) in normal goat serum plus 0.3% Triton X-100 buffer.

Software:

Article Title: Unsaturated Fatty Acids Revert Diet-Induced Hypothalamic Inflammation in Obesity
Article Snippet: After inclusion, 4 mm sections were obtained on a microtome (CUT model 445, Olympus). .. The mean area of adipocytes (average surface area of 30 randomly sorted adipocytes, per animal) was determined by the Imagelab Analysis software (Imagelab, Modena, Italy, v. 2.4).

Article Title: Stable Liposome in Cosmetic Platforms for Transdermal Folic acid delivery for fortification and treatment of micronutrient deficiencies
Article Snippet: Transverse sections of the treated area of skin were sliced using microtome (~10 µm thickness) to generate XZ-planar optical cross-sections and scanned under a confocal laser scanning microscope (Olympus IX 81 with FV 500) for fluorescence signals with an argon laser beam with excitation at 488 nm and emission at 560 nm for Rhodamine-B . .. The intensity of the permeated depth was detected using Fluoview software.

In Situ:

Article Title: Heritable endogenous gene regulation in plants with designed polydactyl zinc finger transcription factors
Article Snippet: .. Stained tissues were dehydrated in an ethanol series and photographed before infiltration with Histo-Clear (International Diagnostics, Atlanta), and paraffin-cast specimen blocks ( ) were sectioned on a microtome (Microm HM315, Mikron) and stained in situ with 0.1% aqueous Safranin-O for 5–60 s. Stained surfaces of specimen blocks were treated with immersion oil and visualized under a dissecting microscope (Olympus, New Hyde Park, NY). .. The target sequence of the designed AP3 -specific ZFP AP3 is an 18-bp sequence located in the AP3 promoter region, at −112 to −95 relative to the ATG codon (Fig. A ).

Laser-Scanning Microscopy:

Article Title: Stable Liposome in Cosmetic Platforms for Transdermal Folic acid delivery for fortification and treatment of micronutrient deficiencies
Article Snippet: .. Transverse sections of the treated area of skin were sliced using microtome (~10 µm thickness) to generate XZ-planar optical cross-sections and scanned under a confocal laser scanning microscope (Olympus IX 81 with FV 500) for fluorescence signals with an argon laser beam with excitation at 488 nm and emission at 560 nm for Rhodamine-B . ..

Concentration Assay:

Article Title: Liposomal paclitaxel induces fewer hematopoietic and cardiovascular complications than bioequivalent doses of Taxol
Article Snippet: Plasma samples were supplied to Taiwan Mouse Clinic to determine blood Ca2+ concentration, alanine aminotransferase (ALT), aspartate aminotransferase (AST), total bilirubin (TBIL), blood urea nitrogen (BUN) and uric acid (UA) using the Fuji Dri-Chem 4000i (Fujifilm, Tokyo, Japan). .. Sections (size, 4 µ m) were cut with a microtome (Olympus Cut 4055; Olympus Corporation, Tokyo, Japan) and mounted on slides.

Article Title: Liposomal paclitaxel induces fewer hematopoietic and cardiovascular complications than bioequivalent doses of Taxol
Article Snippet: Plasma samples were supplied to Taiwan Mouse Clinic to determine blood Ca2+ concentration, alanine aminotransferase (ALT), aspartate aminotransferase (AST), total bilirubin (TBIL), blood urea nitrogen (BUN) and uric acid (UA) using the Fuji Dri-Chem 4000i (Fujifilm, Tokyo, Japan). .. Sections (size, 4 µ m) were cut with a microtome (Olympus Cut 4055; Olympus Corporation, Tokyo, Japan) and mounted on slides.

Cell Counting:

Article Title: Liposomal paclitaxel induces fewer hematopoietic and cardiovascular complications than bioequivalent doses of Taxol
Article Snippet: A total of 1 h after the last injection, whole blood, which was collected in BD Microtainer® blood collection tubes (BD Biosciences) was supplied to Taiwan Mouse Clinic (Taipei, Taiwan) for blood cell count using the IDEXX ProCyte Dx Hematology Analyzer (IDEXX Laboratories, Inc., Westbrook, MA, USA). .. Sections (size, 4 µ m) were cut with a microtome (Olympus Cut 4055; Olympus Corporation, Tokyo, Japan) and mounted on slides.

Article Title: Liposomal paclitaxel induces fewer hematopoietic and cardiovascular complications than bioequivalent doses of Taxol
Article Snippet: A total of 1 h after the last injection, whole blood, which was collected in BD Microtainer® blood collection tubes (BD Biosciences) was supplied to Taiwan Mouse Clinic (Taipei, Taiwan) for blood cell count using the IDEXX ProCyte Dx Hematology Analyzer (IDEXX Laboratories, Inc., Westbrook, MA, USA). .. Sections (size, 4 µ m) were cut with a microtome (Olympus Cut 4055; Olympus Corporation, Tokyo, Japan) and mounted on slides.

Staining:

Article Title: Th2-Associated Alternative Kupffer Cell Activation Promotes Liver Fibrosis without Inducing Local Inflammation
Article Snippet: Once the blocks were solidified after 24 h at room temperature, the liver tissue was cross-sectioned in 4 μM slices using a microtome (Microtome Olympus Cut 4060, USA). .. Liver sections were separately stained with hematoxylin-eosin or Sirius red for evaluation of the inflammatory infiltrate and liver fibrosis, respectively.

Article Title: Study of hepatotoxicity and oxidative stress in male Swiss-Webster mice exposed to functionalized multi-walled carbon nanotubes
Article Snippet: .. The liver specimens were processed and embedded in paraffin, sectioned at 0.1 μm using microtome (Olympus CUT 4055E, USA) and stained with hematoxylin and eosin (H & E). .. The stained tissues were visualized under the microscope and evaluated for the changes in morphology of MWCNTs exposed liver compared with the control.

Article Title: Heritable endogenous gene regulation in plants with designed polydactyl zinc finger transcription factors
Article Snippet: .. Stained tissues were dehydrated in an ethanol series and photographed before infiltration with Histo-Clear (International Diagnostics, Atlanta), and paraffin-cast specimen blocks ( ) were sectioned on a microtome (Microm HM315, Mikron) and stained in situ with 0.1% aqueous Safranin-O for 5–60 s. Stained surfaces of specimen blocks were treated with immersion oil and visualized under a dissecting microscope (Olympus, New Hyde Park, NY). .. The target sequence of the designed AP3 -specific ZFP AP3 is an 18-bp sequence located in the AP3 promoter region, at −112 to −95 relative to the ATG codon (Fig. A ).

Article Title: Apolipoprotein AIV Requires Cholecystokinin and Vagal Nerves to Suppress Food Intake
Article Snippet: .. The frozen brain was sliced with a microtome (cut 4055; Olympus, Tokyo, Japan) and brain sections (30 μm) were stained with diluted c-Fos Ab-5 (1:30,000; Calbiochem, Gibbstown, NJ) and biotinylated goat antirabbit secondary antibody (dilution 1:200) in normal goat serum plus 0.3% Triton X-100 buffer. .. The sections were incubated with horseradish peroxidase avidin-biotin complex (dilution 1:200) and developed by diaminobenzidine with nickel sulfate.

Article Title: Tamoxifen Treatment in Hamsters Induces Protection during Taeniosis by Taenia solium
Article Snippet: After this time, all of the tissues were embedded in paraffin for being posterior cross-sectioned in thin 4 μ M slices, by using a microtome (Microtome Olympus Cut 4060, USA). .. Sections were stained with hematoxylin-eosin for evaluating the inflammatory infiltrate degree on each sample, considered as number of polymorphonuclear leukocytes per ten microvilli, using an optical microscope at 40 and 100x magnification (Nikon Microphot-FXA Microscope).

Article Title: Accumulation of Amyloid Precursor Protein in the Mitochondrial Import Channels of Human Alzheimer’s Disease Brain Is Associated with Mitochondrial Dysfunction
Article Snippet: Paraffin-embedded tissues were cut into 10 μm serial sections using a microtome (Olympus Optical). .. Serial sections from frontal cortex [Broddman’s area 10 (BM10)] were blocked in PBS buffer (containing 0.05% Triton X-100 and 5% donkey serum) and further sequentially stained overnight at 4°C with mouse monoclonal antibodies raised to the N-terminal 18–38 amino acids of APP (APP Nt Ab) (Calbiochem, La Jolla, CA) and rabbit polyclonal anti- translocase of the outer mitochondrial membrane 40 (TOM40) (Santa Cruz Biotechnology, Santa Cruz, CA).

Article Title: Liposomal paclitaxel induces fewer hematopoietic and cardiovascular complications than bioequivalent doses of Taxol
Article Snippet: Sections (size, 4 µ m) were cut with a microtome (Olympus Cut 4055; Olympus Corporation, Tokyo, Japan) and mounted on slides. .. The slides then were subjected to hematoxylin and eosin staining.

Article Title: Effects of limited access dressing in chronic wounds: A biochemical and histological study
Article Snippet: .. Serial sections of 5-μm thickness were cut using microtome (Leica RM2255), stained with haematoxylin-eosin[ ] and examined under light microscopy (Olympus PM20). .. Statistical analysis Statistical analysis was performed using Student's t -test using the SPSS software 15th version package.

Article Title: Unsaturated Fatty Acids Revert Diet-Induced Hypothalamic Inflammation in Obesity
Article Snippet: After inclusion, 4 mm sections were obtained on a microtome (CUT model 445, Olympus). .. Epididymal fat sections were stained with hematoxylin and eosin (Merck).

Article Title: Liposomal paclitaxel induces fewer hematopoietic and cardiovascular complications than bioequivalent doses of Taxol
Article Snippet: Sections (size, 4 µ m) were cut with a microtome (Olympus Cut 4055; Olympus Corporation, Tokyo, Japan) and mounted on slides. .. The slides then were subjected to hematoxylin and eosin staining.

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    Olympus microdome array
    Scanning electron microscope (SEM) images of ( a ) <t>microdome</t> cavities on GC mold and ( b ) microdome array on aluminum (Al) substrate fabricated by DMF with a temperature of 645 °C and a pressure of 2 MPa.
    Microdome Array, supplied by Olympus, used in various techniques. Bioz Stars score: 84/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Scanning electron microscope (SEM) images of ( a ) <t>microdome</t> cavities on GC mold and ( b ) microdome array on aluminum (Al) substrate fabricated by <t>DMF</t> with a temperature of 645 °C and a pressure of 2 MPa.
    Dmf Microdome Al Substrate, supplied by Olympus, used in various techniques. Bioz Stars score: 84/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Olympus microtome
    Scanning electron microscope (SEM) images of ( a ) <t>microdome</t> cavities on GC mold and ( b ) microdome array on aluminum (Al) substrate fabricated by <t>DMF</t> with a temperature of 645 °C and a pressure of 2 MPa.
    Microtome, supplied by Olympus, used in various techniques. Bioz Stars score: 98/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/microtome/product/Olympus
    Average 98 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    microtome - by Bioz Stars, 2020-03
    98/100 stars
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    80
    Olympus cut 4055 microtome
    Scanning electron microscope (SEM) images of ( a ) <t>microdome</t> cavities on GC mold and ( b ) microdome array on aluminum (Al) substrate fabricated by <t>DMF</t> with a temperature of 645 °C and a pressure of 2 MPa.
    Cut 4055 Microtome, supplied by Olympus, used in various techniques. Bioz Stars score: 80/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cut 4055 microtome/product/Olympus
    Average 80 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    cut 4055 microtome - by Bioz Stars, 2020-03
    80/100 stars
      Buy from Supplier

    Image Search Results


    Scanning electron microscope (SEM) images of ( a ) microdome cavities on GC mold and ( b ) microdome array on aluminum (Al) substrate fabricated by DMF with a temperature of 645 °C and a pressure of 2 MPa.

    Journal: Micromachines

    Article Title: Direct Metal Forming of a Microdome Structure with a Glassy Carbon Mold for Enhanced Boiling Heat Transfer

    doi: 10.3390/mi9080376

    Figure Lengend Snippet: Scanning electron microscope (SEM) images of ( a ) microdome cavities on GC mold and ( b ) microdome array on aluminum (Al) substrate fabricated by DMF with a temperature of 645 °C and a pressure of 2 MPa.

    Article Snippet: The measured diameter and pitch of the microdome array on the Al substrate obtained from the SEM image were exactly the same as those of the GC mold. shows the three-dimensional surface profiles of (a) the GC mold and (b) the fabricated DMF microdome Al substrate obtained by a laser confocal microscope (OLS-4100, Olympus.

    Techniques: Microscopy

    Three-dimensional surface profiles of ( a ) a GC mold and ( b ) a DMF microdome Al substrate obtained by laser confocal microscope.

    Journal: Micromachines

    Article Title: Direct Metal Forming of a Microdome Structure with a Glassy Carbon Mold for Enhanced Boiling Heat Transfer

    doi: 10.3390/mi9080376

    Figure Lengend Snippet: Three-dimensional surface profiles of ( a ) a GC mold and ( b ) a DMF microdome Al substrate obtained by laser confocal microscope.

    Article Snippet: The measured diameter and pitch of the microdome array on the Al substrate obtained from the SEM image were exactly the same as those of the GC mold. shows the three-dimensional surface profiles of (a) the GC mold and (b) the fabricated DMF microdome Al substrate obtained by a laser confocal microscope (OLS-4100, Olympus.

    Techniques: Microscopy

    Comparison of the boiling curves of three DMF microdome Al substrates and a bare Al substrate.

    Journal: Micromachines

    Article Title: Direct Metal Forming of a Microdome Structure with a Glassy Carbon Mold for Enhanced Boiling Heat Transfer

    doi: 10.3390/mi9080376

    Figure Lengend Snippet: Comparison of the boiling curves of three DMF microdome Al substrates and a bare Al substrate.

    Article Snippet: The measured diameter and pitch of the microdome array on the Al substrate obtained from the SEM image were exactly the same as those of the GC mold. shows the three-dimensional surface profiles of (a) the GC mold and (b) the fabricated DMF microdome Al substrate obtained by a laser confocal microscope (OLS-4100, Olympus.

    Techniques:

    Scanning electron microscope (SEM) images of ( a ) microdome cavities on GC mold and ( b ) microdome array on aluminum (Al) substrate fabricated by DMF with a temperature of 645 °C and a pressure of 2 MPa.

    Journal: Micromachines

    Article Title: Direct Metal Forming of a Microdome Structure with a Glassy Carbon Mold for Enhanced Boiling Heat Transfer

    doi: 10.3390/mi9080376

    Figure Lengend Snippet: Scanning electron microscope (SEM) images of ( a ) microdome cavities on GC mold and ( b ) microdome array on aluminum (Al) substrate fabricated by DMF with a temperature of 645 °C and a pressure of 2 MPa.

    Article Snippet: The measured diameter and pitch of the microdome array on the Al substrate obtained from the SEM image were exactly the same as those of the GC mold. shows the three-dimensional surface profiles of (a) the GC mold and (b) the fabricated DMF microdome Al substrate obtained by a laser confocal microscope (OLS-4100, Olympus.

    Techniques: Microscopy

    Three-dimensional surface profiles of ( a ) a GC mold and ( b ) a DMF microdome Al substrate obtained by laser confocal microscope.

    Journal: Micromachines

    Article Title: Direct Metal Forming of a Microdome Structure with a Glassy Carbon Mold for Enhanced Boiling Heat Transfer

    doi: 10.3390/mi9080376

    Figure Lengend Snippet: Three-dimensional surface profiles of ( a ) a GC mold and ( b ) a DMF microdome Al substrate obtained by laser confocal microscope.

    Article Snippet: The measured diameter and pitch of the microdome array on the Al substrate obtained from the SEM image were exactly the same as those of the GC mold. shows the three-dimensional surface profiles of (a) the GC mold and (b) the fabricated DMF microdome Al substrate obtained by a laser confocal microscope (OLS-4100, Olympus.

    Techniques: Microscopy

    Comparison of the boiling curves of three DMF microdome Al substrates and a bare Al substrate.

    Journal: Micromachines

    Article Title: Direct Metal Forming of a Microdome Structure with a Glassy Carbon Mold for Enhanced Boiling Heat Transfer

    doi: 10.3390/mi9080376

    Figure Lengend Snippet: Comparison of the boiling curves of three DMF microdome Al substrates and a bare Al substrate.

    Article Snippet: The measured diameter and pitch of the microdome array on the Al substrate obtained from the SEM image were exactly the same as those of the GC mold. shows the three-dimensional surface profiles of (a) the GC mold and (b) the fabricated DMF microdome Al substrate obtained by a laser confocal microscope (OLS-4100, Olympus.

    Techniques: