Structured Review

Leica Microsystems microtome
Microtome, supplied by Leica Microsystems, used in various techniques. Bioz Stars score: 99/100, based on 278 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/microtome/product/Leica Microsystems
Average 99 stars, based on 278 article reviews
Price from $9.99 to $1999.99
microtome - by Bioz Stars, 2020-03
99/100 stars

Images

Related Articles

Immunostaining:

Article Title: Penfluridol: an antipsychotic agent suppresses metastatic tumor growth in triple negative breast cancer by inhibiting integrin signaling axis
Article Snippet: Paragraph title: Immunostaining of brain sections ... Briefly, fixed brains were dehydrated, embedded in paraffin and sectioned into 5µm thick sections using microtome (Leica Microsystems Inc., Buffalo Grove, IL).

Article Title: Beneficial Effects of Remifentanil Against Excitotoxic Brain Damage in Newborn Mice
Article Snippet: Slices (7-μm thick) immediately adjacent to the site of the ic injection were cut on a microtome (Leica 2035 Biocut, Leica Microsystems, Wetzlar, Germany). .. Immunostaining of the astrocyte marker GFAP was performed using a goat anti-GFAP (sc-6170, dilution 1:200, Santa Cruz Biotechnology, Dallas, TX, USA) successively and an Alexa Fluor® 594 donkey anti-goat IgG (A-11058, dilution 1:400, Invitrogen, Carlsbad, CA, USA).

Article Title: Deficiency of Schnurri-2, an MHC Enhancer Binding Protein, Induces Mild Chronic Inflammation in the Brain and Confers Molecular, Neuronal, and Behavioral Phenotypes Related to Schizophrenia
Article Snippet: The brain samples were mounted in Tissue-Tek (Miles, Elkhart, IN), frozen, and cut into 50-μm-thick coronal sections using a microtome (CM1850, Leica Microsystems, Wetzlar, Germany). .. For immunostaining, the cryostat sections were incubated at 4 °C for 18 h with the following primary antibodies: calbindin (mouse monoclonal antibody 300 and rabbit polyclonal antibody D-28k; Swant, Bellinzona, Switzerland), calretinin (mouse monoclonal antibody 6B3 and rabbit polyclonal antibody 7699/4; Swant), GAD67 (MAB5406; Millipore, Temecula, CA), mouse anti-CNPase monoclonal (C5922; Sigma-Aldrich, St Louis, MO), GFAP (G9269; Sigma-Aldrich), p22 phox (sc-20781; Santa Cruz Biotechnology, San Diego, CA), goat polyclonal antibody for parvalbumin (PVG-214; Swant).

Incubation:

Article Title: Membrane Properties and the Balance between Excitation and Inhibition Control Gamma-Frequency Oscillations Arising from Feedback Inhibition
Article Snippet: Coronal slices were cut to a thickness of 400 µm with a vibrating microtome (VT1200; Leica Microsystems). .. Slices were then incubated at room temperature (∼22°C) in oxygenated aCSF for 60 min. After the incubation period, slices were moved to the stage of a custom-built microscope equipped with brightfield and two-photon fluorescence optics.

Article Title: Intramyocardially Transplanted Neonatal Cardiomyocytes (NCMs) Show Structural and Electrophysiological Maturation and Integration and Dose-Dependently Stabilize Function of Infarcted Rat Hearts
Article Snippet: Briefly, ventricles were embedded in 4% low-melt agarose (Carl Roth, Karlsruhe, Germany) suspended in Tyrode's solution (composition in mmol/L: NaCl 136, KCl 5.4, NaH2 PO4 0.33, MgCl2 1, d -glucose 10, HEPES 5, 2,3-butanedione monoxime 30; pH 7.4 adjusted with NaOH; Sigma-Aldrich), and 200-μm-thick short-axis slices were cut with a microtome (Leica VT1000S; Leica Microsystems, Wetzlar, Germany). .. Slices were incubated in DMEM at 37°C with 95% O2 and 5% CO2 for another 30 min. Electrophysiological measurements were performed at 37°C and pH 7.4 in culture medium aerated with carbogen.

Article Title: Penfluridol: an antipsychotic agent suppresses metastatic tumor growth in triple negative breast cancer by inhibiting integrin signaling axis
Article Snippet: Briefly, fixed brains were dehydrated, embedded in paraffin and sectioned into 5µm thick sections using microtome (Leica Microsystems Inc., Buffalo Grove, IL). .. Antigens were unmasked by boiling the sections in 10 mM sodium citrate buffer (pH 6.0) and the sections were washed and incubated in 3% hydrogen peroxide solution.

Article Title: Radixin inhibition decreases adult neural progenitor cell migration and proliferation in vitro and in vivo
Article Snippet: Sagittal sections were cut at 25 μm and coronal sections were cut at 40 μm on a sliding microtome (Leica Microsystems, Wetzlar, Germany) followed by immunofluorescence. .. Sections were blocked for 30 min in 3% normal donkey serum (Jackson ImmunoResearch, West Grove, PA, USA) in 0.1% Triton X-100, and then incubated for 48 h at 4°C in primary antibodies; monoclonal rabbit anti-radixin (Abcam, Cambridge, MA, USA), mouse anti-radixin (Abnova, Taipei City, Taiwan), rabbit anti-phosphorylated ezrin/radixin/moesin (Cell signaling, Danvers, MA, USA), rabbit anti-phosphorylated histone H3 (PHH3, Millipore, Billerica, MA, USA), mouse IgM anti-PSA-NCAM (Chemicon International/Millipore, Billerica, MA, USA).

Article Title: S6K1 in the Central Nervous System Regulates Energy Expenditure via MC4R/CRH Pathways in Response to Deprivation of an Essential Amino Acid
Article Snippet: .. In brief, brain coronal sections of 25 μm were cut using a frozen microtome (Leica Microsystems, Bensheim, Germany) and incubated with primary antibody (anti–p-S6 [Ser235/236]; Cell Signaling Technology), and pictures were taken by using an Olympus BX61 microscope (Olympus, Tokyo, Japan). .. Immunofluorescence staining.

Article Title: Deficiency of Schnurri-2, an MHC Enhancer Binding Protein, Induces Mild Chronic Inflammation in the Brain and Confers Molecular, Neuronal, and Behavioral Phenotypes Related to Schizophrenia
Article Snippet: The brain samples were mounted in Tissue-Tek (Miles, Elkhart, IN), frozen, and cut into 50-μm-thick coronal sections using a microtome (CM1850, Leica Microsystems, Wetzlar, Germany). .. For immunostaining, the cryostat sections were incubated at 4 °C for 18 h with the following primary antibodies: calbindin (mouse monoclonal antibody 300 and rabbit polyclonal antibody D-28k; Swant, Bellinzona, Switzerland), calretinin (mouse monoclonal antibody 6B3 and rabbit polyclonal antibody 7699/4; Swant), GAD67 (MAB5406; Millipore, Temecula, CA), mouse anti-CNPase monoclonal (C5922; Sigma-Aldrich, St Louis, MO), GFAP (G9269; Sigma-Aldrich), p22 phox (sc-20781; Santa Cruz Biotechnology, San Diego, CA), goat polyclonal antibody for parvalbumin (PVG-214; Swant).

Activity Assay:

Article Title: Membrane Properties and the Balance between Excitation and Inhibition Control Gamma-Frequency Oscillations Arising from Feedback Inhibition
Article Snippet: Coronal slices were cut to a thickness of 400 µm with a vibrating microtome (VT1200; Leica Microsystems). .. Slices were bathed in standard aCSF with 10 µM CNQX and 50 µM picrotoxin added in some experiments to block synaptic activity.

Transplantation Assay:

Article Title: Intramyocardially Transplanted Neonatal Cardiomyocytes (NCMs) Show Structural and Electrophysiological Maturation and Integration and Dose-Dependently Stabilize Function of Infarcted Rat Hearts
Article Snippet: To study electrical integration and electrophysiological properties of transplanted NCMs, noninfarcted recipients were sacrificed 3 or 6 days after intramyocardial transplantation. .. Briefly, ventricles were embedded in 4% low-melt agarose (Carl Roth, Karlsruhe, Germany) suspended in Tyrode's solution (composition in mmol/L: NaCl 136, KCl 5.4, NaH2 PO4 0.33, MgCl2 1, d -glucose 10, HEPES 5, 2,3-butanedione monoxime 30; pH 7.4 adjusted with NaOH; Sigma-Aldrich), and 200-μm-thick short-axis slices were cut with a microtome (Leica VT1000S; Leica Microsystems, Wetzlar, Germany).

Western Blot:

Article Title: Beneficial Effects of Remifentanil Against Excitotoxic Brain Damage in Newborn Mice
Article Snippet: Paragraph title: GFAP Immunohistochemistry and Western Blot ... Slices (7-μm thick) immediately adjacent to the site of the ic injection were cut on a microtome (Leica 2035 Biocut, Leica Microsystems, Wetzlar, Germany).

Magnetic Resonance Imaging:

Article Title: Multi-modal imaging and analysis in the search for iron-based magnetoreceptors in the honeybee Apis mellifera
Article Snippet: Scanning electron microscopy and microanalysis A single eclosed and 25 day old abdomen was selected from those imaged using MRI and prepared for scanning electron microscopy (SEM) and X-ray microanalysis (i.e. energy dispersive spectroscopy, EDS). .. Final orientations were obtained by mounting the samples in a microtome (EM UC6, Leica Microsystems) and trimming away resin with a glass knife.

TUNEL Assay:

Article Title: MicroRNA-325-3p protects the heart after myocardial infarction by inhibiting RIPK3 and programmed necrosis in mice
Article Snippet: Paragraph title: Histopathological examination and TUNEL staining ... The paraffin blocks were cut into 4 μm sections using a microtome (Leica M650; Leica Microsystems GmbH, Wetzlar, Germany), and the sections were stained with hematoxylin and eosin (H & E, Solarbio) for histopathology and Masson’s trichrome (Sigma-Aldrich, St. Louis, MO, USA) for fibrosis.

Electron Microscopy:

Article Title: Multi-modal imaging and analysis in the search for iron-based magnetoreceptors in the honeybee Apis mellifera
Article Snippet: Paragraph title: Scanning electron microscopy and microanalysis ... Final orientations were obtained by mounting the samples in a microtome (EM UC6, Leica Microsystems) and trimming away resin with a glass knife.

Immunohistochemistry:

Article Title: Stress Reaction in Outer Segments of Photoreceptors after Blue Light Irradiation
Article Snippet: Paragraph title: Immunohistochemistry ... Sagittal sections of the eyecups were cut at 7 µm with a microtome (RM2065; Leica Microsystems, Wetzlar, Germany).

Article Title: Beneficial Effects of Remifentanil Against Excitotoxic Brain Damage in Newborn Mice
Article Snippet: Paragraph title: GFAP Immunohistochemistry and Western Blot ... Slices (7-μm thick) immediately adjacent to the site of the ic injection were cut on a microtome (Leica 2035 Biocut, Leica Microsystems, Wetzlar, Germany).

Article Title: S6K1 in the Central Nervous System Regulates Energy Expenditure via MC4R/CRH Pathways in Response to Deprivation of an Essential Amino Acid
Article Snippet: Paragraph title: Immunohistochemistry staining. ... In brief, brain coronal sections of 25 μm were cut using a frozen microtome (Leica Microsystems, Bensheim, Germany) and incubated with primary antibody (anti–p-S6 [Ser235/236]; Cell Signaling Technology), and pictures were taken by using an Olympus BX61 microscope (Olympus, Tokyo, Japan).

Article Title: Deficiency of Schnurri-2, an MHC Enhancer Binding Protein, Induces Mild Chronic Inflammation in the Brain and Confers Molecular, Neuronal, and Behavioral Phenotypes Related to Schizophrenia
Article Snippet: Paragraph title: Immunohistochemistry ... The brain samples were mounted in Tissue-Tek (Miles, Elkhart, IN), frozen, and cut into 50-μm-thick coronal sections using a microtome (CM1850, Leica Microsystems, Wetzlar, Germany).

Article Title: Exosomes Secreted from Human-Induced Pluripotent Stem Cell-Derived Mesenchymal Stem Cells Prevent Osteonecrosis of the Femoral Head by Promoting Angiogenesis
Article Snippet: Paragraph title: 2.4. Histological and immunohistochemical (IHC) analysis ... The embedded samples were cut into 5 μm sections with a microtome (Leica Microsystems, Wetzlar, Germany).

Light Microscopy:

Article Title: MicroRNA-325-3p protects the heart after myocardial infarction by inhibiting RIPK3 and programmed necrosis in mice
Article Snippet: The paraffin blocks were cut into 4 μm sections using a microtome (Leica M650; Leica Microsystems GmbH, Wetzlar, Germany), and the sections were stained with hematoxylin and eosin (H & E, Solarbio) for histopathology and Masson’s trichrome (Sigma-Aldrich, St. Louis, MO, USA) for fibrosis. .. The paraffin blocks were cut into 4 μm sections using a microtome (Leica M650; Leica Microsystems GmbH, Wetzlar, Germany), and the sections were stained with hematoxylin and eosin (H & E, Solarbio) for histopathology and Masson’s trichrome (Sigma-Aldrich, St. Louis, MO, USA) for fibrosis.

Article Title: Exosomes Secreted from Human-Induced Pluripotent Stem Cell-Derived Mesenchymal Stem Cells Prevent Osteonecrosis of the Femoral Head by Promoting Angiogenesis
Article Snippet: The embedded samples were cut into 5 μm sections with a microtome (Leica Microsystems, Wetzlar, Germany). .. Sections were stained with hematoxylin and eosin (HE) and visualized under a light microscope (Leica Microsystems).

Imaging:

Article Title: Multi-modal imaging and analysis in the search for iron-based magnetoreceptors in the honeybee Apis mellifera
Article Snippet: Final orientations were obtained by mounting the samples in a microtome (EM UC6, Leica Microsystems) and trimming away resin with a glass knife. .. For SEM imaging and EDS analysis, samples were examined in a field emission SEM (1555VP, Zeiss) fitted with an X-ray analytical system (Oxford Instruments, X-Max 80 SDD) with thin window detector.

Injection:

Article Title: Beneficial Effects of Remifentanil Against Excitotoxic Brain Damage in Newborn Mice
Article Snippet: .. Slices (7-μm thick) immediately adjacent to the site of the ic injection were cut on a microtome (Leica 2035 Biocut, Leica Microsystems, Wetzlar, Germany). .. Immunostaining of the astrocyte marker GFAP was performed using a goat anti-GFAP (sc-6170, dilution 1:200, Santa Cruz Biotechnology, Dallas, TX, USA) successively and an Alexa Fluor® 594 donkey anti-goat IgG (A-11058, dilution 1:400, Invitrogen, Carlsbad, CA, USA).

Article Title: Dedicated C‐fibre viscerosensory pathways to central nucleus of the amygdala
Article Snippet: Briefly, adult rats (344 ± 11 g) were deeply anaesthetized with isoflurane (5%) and the brainstem was removed 10–30 days following amygdala tracer injection. .. Slices were cut with a sapphire knife (Delaware Diamond Knives, Wilmington, DE, USA) mounted in a vibrating microtome (VT1000S; Leica Microsystems Inc., Bannockburn, IL, USA).

Immunofluorescence:

Article Title: Radixin inhibition decreases adult neural progenitor cell migration and proliferation in vitro and in vivo
Article Snippet: .. Sagittal sections were cut at 25 μm and coronal sections were cut at 40 μm on a sliding microtome (Leica Microsystems, Wetzlar, Germany) followed by immunofluorescence. ..

Histopathology:

Article Title: MicroRNA-325-3p protects the heart after myocardial infarction by inhibiting RIPK3 and programmed necrosis in mice
Article Snippet: .. The paraffin blocks were cut into 4 μm sections using a microtome (Leica M650; Leica Microsystems GmbH, Wetzlar, Germany), and the sections were stained with hematoxylin and eosin (H & E, Solarbio) for histopathology and Masson’s trichrome (Sigma-Aldrich, St. Louis, MO, USA) for fibrosis. .. The histology of the myocardium was examined with light microscopy (Olympus, Tokyo, Japan).

Fluorescence:

Article Title: Membrane Properties and the Balance between Excitation and Inhibition Control Gamma-Frequency Oscillations Arising from Feedback Inhibition
Article Snippet: Coronal slices were cut to a thickness of 400 µm with a vibrating microtome (VT1200; Leica Microsystems). .. Slices were then incubated at room temperature (∼22°C) in oxygenated aCSF for 60 min. After the incubation period, slices were moved to the stage of a custom-built microscope equipped with brightfield and two-photon fluorescence optics.

Labeling:

Article Title: Membrane Properties and the Balance between Excitation and Inhibition Control Gamma-Frequency Oscillations Arising from Feedback Inhibition
Article Snippet: Tissue preparation Coronal sections of neocortex were prepared from 15- to 28-day old G42 mice in which GABAergic, parvalbumin-positive interneurons are fluorescently labeled with GFP. .. Coronal slices were cut to a thickness of 400 µm with a vibrating microtome (VT1200; Leica Microsystems).

Article Title: Transient Hypoxemia Chronically Disrupts Maturation of Preterm Fetal Ovine Subplate Neuron Arborization and Activity
Article Snippet: The hemisphere destined for morphometric studies was subdivided into two equivalent coronal blocks (labeled A and B, where A is most anterior), and immersed in 4% PFA in 0.1 m phosphate buffer (pH 7.4, 4°C). .. B-blocks were removed from fixative 3 h after immersion and were cut into 200 μm coronal slices using a vibrating microtome (VT1000S, Leica Microsystems).

Mouse Assay:

Article Title: Membrane Properties and the Balance between Excitation and Inhibition Control Gamma-Frequency Oscillations Arising from Feedback Inhibition
Article Snippet: G42 transgenic mice were obtained from The Jackson Laboratory ( http://www.jax.org ). .. Coronal slices were cut to a thickness of 400 µm with a vibrating microtome (VT1200; Leica Microsystems).

Article Title: Deficiency of Schnurri-2, an MHC Enhancer Binding Protein, Induces Mild Chronic Inflammation in the Brain and Confers Molecular, Neuronal, and Behavioral Phenotypes Related to Schizophrenia
Article Snippet: Immunohistochemistry Mice were deeply anesthetized with chloral hydrate (245 mg/kg, i.p.) and transcardially perfused with 4% paraformaldehyde in 0.1 M phosphate buffer, pH 7.4. .. The brain samples were mounted in Tissue-Tek (Miles, Elkhart, IN), frozen, and cut into 50-μm-thick coronal sections using a microtome (CM1850, Leica Microsystems, Wetzlar, Germany).

Transgenic Assay:

Article Title: Membrane Properties and the Balance between Excitation and Inhibition Control Gamma-Frequency Oscillations Arising from Feedback Inhibition
Article Snippet: G42 transgenic mice were obtained from The Jackson Laboratory ( http://www.jax.org ). .. Coronal slices were cut to a thickness of 400 µm with a vibrating microtome (VT1200; Leica Microsystems).

Blocking Assay:

Article Title: Membrane Properties and the Balance between Excitation and Inhibition Control Gamma-Frequency Oscillations Arising from Feedback Inhibition
Article Snippet: Coronal slices were cut to a thickness of 400 µm with a vibrating microtome (VT1200; Leica Microsystems). .. Slices were bathed in standard aCSF with 10 µM CNQX and 50 µM picrotoxin added in some experiments to block synaptic activity.

Article Title: Dedicated C‐fibre viscerosensory pathways to central nucleus of the amygdala
Article Snippet: The medulla was rapidly cooled and trimmed rostral and caudal to yield a brainstem block centreed on obex. .. Slices were cut with a sapphire knife (Delaware Diamond Knives, Wilmington, DE, USA) mounted in a vibrating microtome (VT1000S; Leica Microsystems Inc., Bannockburn, IL, USA).

Article Title: Multi-modal imaging and analysis in the search for iron-based magnetoreceptors in the honeybee Apis mellifera
Article Snippet: Final orientations were obtained by mounting the samples in a microtome (EM UC6, Leica Microsystems) and trimming away resin with a glass knife. .. Block surfaces were polished using a diamond knife (45° Histo, Diatome), then mounted onto aluminium stubs with carbon paste and coated with 20 nm carbon with an evaporative coater (Speedivac 12E6, Edwards High Vacuum Ltd, UK).

Article Title: Transient Hypoxemia Chronically Disrupts Maturation of Preterm Fetal Ovine Subplate Neuron Arborization and Activity
Article Snippet: This block has been analyzed extensively by us in prior studies ( ; ) because of the predilection for cerebral WM injury at this level both in our fetal sheep preparations and in human preterm infants. .. B-blocks were removed from fixative 3 h after immersion and were cut into 200 μm coronal slices using a vibrating microtome (VT1000S, Leica Microsystems).

Spectroscopy:

Article Title: Multi-modal imaging and analysis in the search for iron-based magnetoreceptors in the honeybee Apis mellifera
Article Snippet: Scanning electron microscopy and microanalysis A single eclosed and 25 day old abdomen was selected from those imaged using MRI and prepared for scanning electron microscopy (SEM) and X-ray microanalysis (i.e. energy dispersive spectroscopy, EDS). .. Final orientations were obtained by mounting the samples in a microtome (EM UC6, Leica Microsystems) and trimming away resin with a glass knife.

Chloramphenicol Acetyltransferase Assay:

Article Title: Effect of crocin on cardiac antioxidants, and hemodynamic parameters after injuries induced by hepatic ischemia-reperfusion in rats
Article Snippet: Determination of the activity of antioxidants The activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPX ) in heart’s homogenate were determined according to the following method. .. Seventy two hour after fixation, samples were dehydrated using graded alcohol, embedded in paraffin, cut into 5 µm sections by a microtome (Leica RM 2125, Leica Microsystems Nussloch GmbH, Germany), and then stained with Hematoxylin and Eosin ( ).

Microscopy:

Article Title: Membrane Properties and the Balance between Excitation and Inhibition Control Gamma-Frequency Oscillations Arising from Feedback Inhibition
Article Snippet: Coronal slices were cut to a thickness of 400 µm with a vibrating microtome (VT1200; Leica Microsystems). .. Slices were then incubated at room temperature (∼22°C) in oxygenated aCSF for 60 min. After the incubation period, slices were moved to the stage of a custom-built microscope equipped with brightfield and two-photon fluorescence optics.

Article Title: Cell type-specific tonic GABA signaling in the rat central amygdala is selectively altered by acute and chronic ethanol
Article Snippet: Brains were cut into transverse sections (300 μm) on a vibrating microtome (Leica VT1000S, Leica Microsystems, Buffalo Grove, IL) and placed in an oxygenated (95% O2 /5% CO2 ) artificial cerebrospinal fluid (aCSF) solution composed of the following (in mM): NaCl 120; KCl 2.5; EGTA 5; CaCl2 2 MgCl2 1; NaH2 PO4 1.2; NaHCO3 26; Glucose 1.75; HEPES 5. .. Following equilibration, a single slice was transferred to a recording chamber mounted on the stage of an upright microscope (Olympus BX50WI).

Article Title: S6K1 in the Central Nervous System Regulates Energy Expenditure via MC4R/CRH Pathways in Response to Deprivation of an Essential Amino Acid
Article Snippet: .. In brief, brain coronal sections of 25 μm were cut using a frozen microtome (Leica Microsystems, Bensheim, Germany) and incubated with primary antibody (anti–p-S6 [Ser235/236]; Cell Signaling Technology), and pictures were taken by using an Olympus BX61 microscope (Olympus, Tokyo, Japan). .. Immunofluorescence staining.

In Situ:

Article Title: MicroRNA-325-3p protects the heart after myocardial infarction by inhibiting RIPK3 and programmed necrosis in mice
Article Snippet: The paraffin blocks were cut into 4 μm sections using a microtome (Leica M650; Leica Microsystems GmbH, Wetzlar, Germany), and the sections were stained with hematoxylin and eosin (H & E, Solarbio) for histopathology and Masson’s trichrome (Sigma-Aldrich, St. Louis, MO, USA) for fibrosis. .. The heart sections were also used to assess myocardial cell apoptosis through an in situ terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) assay (Roche Diagnostics, Mannheim, Germany).

Sampling:

Article Title: Transient Hypoxemia Chronically Disrupts Maturation of Preterm Fetal Ovine Subplate Neuron Arborization and Activity
Article Snippet: B-blocks were removed from fixative 3 h after immersion and were cut into 200 μm coronal slices using a vibrating microtome (VT1000S, Leica Microsystems). .. Sections were collected at equivalent intervals across the B-block to ensure systematic sampling across the entire block.

Slice Preparation:

Article Title: Cell type-specific tonic GABA signaling in the rat central amygdala is selectively altered by acute and chronic ethanol
Article Snippet: Paragraph title: Brain slice preparation ... Brains were cut into transverse sections (300 μm) on a vibrating microtome (Leica VT1000S, Leica Microsystems, Buffalo Grove, IL) and placed in an oxygenated (95% O2 /5% CO2 ) artificial cerebrospinal fluid (aCSF) solution composed of the following (in mM): NaCl 120; KCl 2.5; EGTA 5; CaCl2 2 MgCl2 1; NaH2 PO4 1.2; NaHCO3 26; Glucose 1.75; HEPES 5.

Article Title: Dedicated C‐fibre viscerosensory pathways to central nucleus of the amygdala
Article Snippet: Paragraph title: Brainstem slice preparation ... Slices were cut with a sapphire knife (Delaware Diamond Knives, Wilmington, DE, USA) mounted in a vibrating microtome (VT1000S; Leica Microsystems Inc., Bannockburn, IL, USA).

End Labeling:

Article Title: MicroRNA-325-3p protects the heart after myocardial infarction by inhibiting RIPK3 and programmed necrosis in mice
Article Snippet: The paraffin blocks were cut into 4 μm sections using a microtome (Leica M650; Leica Microsystems GmbH, Wetzlar, Germany), and the sections were stained with hematoxylin and eosin (H & E, Solarbio) for histopathology and Masson’s trichrome (Sigma-Aldrich, St. Louis, MO, USA) for fibrosis. .. The heart sections were also used to assess myocardial cell apoptosis through an in situ terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) assay (Roche Diagnostics, Mannheim, Germany).

Marker:

Article Title: Beneficial Effects of Remifentanil Against Excitotoxic Brain Damage in Newborn Mice
Article Snippet: Slices (7-μm thick) immediately adjacent to the site of the ic injection were cut on a microtome (Leica 2035 Biocut, Leica Microsystems, Wetzlar, Germany). .. Immunostaining of the astrocyte marker GFAP was performed using a goat anti-GFAP (sc-6170, dilution 1:200, Santa Cruz Biotechnology, Dallas, TX, USA) successively and an Alexa Fluor® 594 donkey anti-goat IgG (A-11058, dilution 1:400, Invitrogen, Carlsbad, CA, USA).

Staining:

Article Title: MicroRNA-325-3p protects the heart after myocardial infarction by inhibiting RIPK3 and programmed necrosis in mice
Article Snippet: .. The paraffin blocks were cut into 4 μm sections using a microtome (Leica M650; Leica Microsystems GmbH, Wetzlar, Germany), and the sections were stained with hematoxylin and eosin (H & E, Solarbio) for histopathology and Masson’s trichrome (Sigma-Aldrich, St. Louis, MO, USA) for fibrosis. .. The histology of the myocardium was examined with light microscopy (Olympus, Tokyo, Japan).

Article Title: Effect of crocin on cardiac antioxidants, and hemodynamic parameters after injuries induced by hepatic ischemia-reperfusion in rats
Article Snippet: .. Seventy two hour after fixation, samples were dehydrated using graded alcohol, embedded in paraffin, cut into 5 µm sections by a microtome (Leica RM 2125, Leica Microsystems Nussloch GmbH, Germany), and then stained with Hematoxylin and Eosin ( ). ..

Article Title: S6K1 in the Central Nervous System Regulates Energy Expenditure via MC4R/CRH Pathways in Response to Deprivation of an Essential Amino Acid
Article Snippet: Paragraph title: Immunohistochemistry staining. ... In brief, brain coronal sections of 25 μm were cut using a frozen microtome (Leica Microsystems, Bensheim, Germany) and incubated with primary antibody (anti–p-S6 [Ser235/236]; Cell Signaling Technology), and pictures were taken by using an Olympus BX61 microscope (Olympus, Tokyo, Japan).

Article Title: Multi-modal imaging and analysis in the search for iron-based magnetoreceptors in the honeybee Apis mellifera
Article Snippet: Abdomens were dehydrated using a graded series of EtOH as described for PB staining and then infiltrated with epoxy resin (Procure 812, Proscitech) mixed with 100% EtOH at ratios of 1 : 3, 1 : 1, 3 : 1 and then 2× 100% resin, for approximately 24 h each on a rotator. .. Final orientations were obtained by mounting the samples in a microtome (EM UC6, Leica Microsystems) and trimming away resin with a glass knife.

Article Title: Exosomes Secreted from Human-Induced Pluripotent Stem Cell-Derived Mesenchymal Stem Cells Prevent Osteonecrosis of the Femoral Head by Promoting Angiogenesis
Article Snippet: The embedded samples were cut into 5 μm sections with a microtome (Leica Microsystems, Wetzlar, Germany). .. Sections were stained with hematoxylin and eosin (HE) and visualized under a light microscope (Leica Microsystems).

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 80
    Leica Microsystems sledge cryo microtome
    Imaging cardiovascular geometries with <t>cryo-EFIC.</t> a) The cryo-EFIC machine and its components. b) Sample EFIC image slice of a newborn mouse, demonstrating that there were contrasts between different tissues to distinguish the organs. Int - intestines; c) Demonstration of segmentation: rendered image of the blood volumes (gray) of a newborn mouse, with the aorta and its head and neck arterial branches set apart in red. d) Segmented great arterial geometries of wildtype fetal mice over stages E14.5 to E18.5 compared to that of the newborn mouse. IVC – inferior vena cavae; Li – liver; Lu – lungs, RA - right ventricle; RV – right ventricle; Sp – spine; AAo: ascending aorta; DAo: descending aorta; PT: pulmonary trunk; Duc: ductus arteriosus; Innom: innominate; LCC: left common carotid; LSC: left subclavian.
    Sledge Cryo Microtome, supplied by Leica Microsystems, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sledge cryo microtome/product/Leica Microsystems
    Average 80 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sledge cryo microtome - by Bioz Stars, 2020-03
    80/100 stars
      Buy from Supplier

    99
    Leica Microsystems microtome
    Imaging cardiovascular geometries with <t>cryo-EFIC.</t> a) The cryo-EFIC machine and its components. b) Sample EFIC image slice of a newborn mouse, demonstrating that there were contrasts between different tissues to distinguish the organs. Int - intestines; c) Demonstration of segmentation: rendered image of the blood volumes (gray) of a newborn mouse, with the aorta and its head and neck arterial branches set apart in red. d) Segmented great arterial geometries of wildtype fetal mice over stages E14.5 to E18.5 compared to that of the newborn mouse. IVC – inferior vena cavae; Li – liver; Lu – lungs, RA - right ventricle; RV – right ventricle; Sp – spine; AAo: ascending aorta; DAo: descending aorta; PT: pulmonary trunk; Duc: ductus arteriosus; Innom: innominate; LCC: left common carotid; LSC: left subclavian.
    Microtome, supplied by Leica Microsystems, used in various techniques. Bioz Stars score: 99/100, based on 278 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/microtome/product/Leica Microsystems
    Average 99 stars, based on 278 article reviews
    Price from $9.99 to $1999.99
    microtome - by Bioz Stars, 2020-03
    99/100 stars
      Buy from Supplier

    Image Search Results


    Imaging cardiovascular geometries with cryo-EFIC. a) The cryo-EFIC machine and its components. b) Sample EFIC image slice of a newborn mouse, demonstrating that there were contrasts between different tissues to distinguish the organs. Int - intestines; c) Demonstration of segmentation: rendered image of the blood volumes (gray) of a newborn mouse, with the aorta and its head and neck arterial branches set apart in red. d) Segmented great arterial geometries of wildtype fetal mice over stages E14.5 to E18.5 compared to that of the newborn mouse. IVC – inferior vena cavae; Li – liver; Lu – lungs, RA - right ventricle; RV – right ventricle; Sp – spine; AAo: ascending aorta; DAo: descending aorta; PT: pulmonary trunk; Duc: ductus arteriosus; Innom: innominate; LCC: left common carotid; LSC: left subclavian.

    Journal: PLoS ONE

    Article Title: Characterizaton of the Vessel Geometry, Flow Mechanics and Wall Shear Stress in the Great Arteries of Wildtype Prenatal Mouse

    doi: 10.1371/journal.pone.0086878

    Figure Lengend Snippet: Imaging cardiovascular geometries with cryo-EFIC. a) The cryo-EFIC machine and its components. b) Sample EFIC image slice of a newborn mouse, demonstrating that there were contrasts between different tissues to distinguish the organs. Int - intestines; c) Demonstration of segmentation: rendered image of the blood volumes (gray) of a newborn mouse, with the aorta and its head and neck arterial branches set apart in red. d) Segmented great arterial geometries of wildtype fetal mice over stages E14.5 to E18.5 compared to that of the newborn mouse. IVC – inferior vena cavae; Li – liver; Lu – lungs, RA - right ventricle; RV – right ventricle; Sp – spine; AAo: ascending aorta; DAo: descending aorta; PT: pulmonary trunk; Duc: ductus arteriosus; Innom: innominate; LCC: left common carotid; LSC: left subclavian.

    Article Snippet: The EFIC machine consisted of a sledge cryo-microtome (CM3500 Cryopolycut, Leica Microsystems GmbH., Germany) custom attached to a mercury lamp fluorescence microscope (MZ16 FA Fluorescent Stereomicroscope, Leica Microsystems GmbH., Germany).

    Techniques: Imaging, Mouse Assay