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Leica Biosystems microtome
Microtome, supplied by Leica Biosystems, used in various techniques. Bioz Stars score: 99/100, based on 62 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/microtome/product/Leica Biosystems
Average 99 stars, based on 62 article reviews
Price from $9.99 to $1999.99
microtome - by Bioz Stars, 2020-03
99/100 stars

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Related Articles

Histopathology:

Article Title: Comparative Study on Pulmonary Toxicity in Mice Induced by Exposure to Unflavoured and Apple- and Strawberry-Flavoured Tobacco Waterpipe Smoke
Article Snippet: Paragraph title: 2.4. Histopathology ... Sections of 5 μ m (7 sections per lung) were cut with a microtome (RM2125 RTS, Leica Biosystems, Nussloch, Germany) and stained with hematoxylin and eosin [ , ].

Article Title: Anti-obesity and fatty liver-preventing activities of Lonicera caerulea in high-fat diet-fed mice
Article Snippet: Paragraph title: Histopathology ... Following embedding of the organs in paraffin using an automated tissue processor (Shandon Citadel 2000; Thermo Fisher Scientific, Inc.) and embedding center (Shandon Histocentre 3; Thermo Fisher Scientific, Inc.), 3-4-µ m serial sections were prepared using a microtome (RM2255; Leica Biosystems, Wetzlar, Germany).

Electron Microscopy:

Article Title: Histological and Immunofluorescent Analysis of a Large Tributary of the Great Saphenous Vein Treated with a 1920 nm Endovenous Laser: Preliminary Findings
Article Snippet: A microtome (Reichert 2040 Microtome, Leica Biosystems, Milton Keynes, UK) was then used to cut 5 μm sections and these were placed onto Superfrost slides. .. Images were taken at ×40, ×100, and ×200 magnifications using an EVOS microscope (Electron Microscopy Sciences, Hatfield, PA, USA).

Immunohistochemistry:

Article Title: Interleukin-1β signaling in fenestrated capillaries is sufficient to trigger sickness responses in mice
Article Snippet: .. Immunohistochemistry (IHC) Cryopreserved frozen brains were sectioned at 30 μm and collected in three serial sets on a sliding microtome (Leica Biosystems, Buffalo Grove, Illinois; SM2000R) equipped with a freezing stage (Physitemp Instruments, Clifton, New Jersey; BFS-5MP). .. Brain sections were first washed with three changes of PBS then pretreated by quenching with 1% glycine (Sigma-Aldrich, Cat#G8898) in PBS and de-fixing in 0.05% sodium borohydride (Sigma-Aldrich, Cat#71321) in PBS, with three PBS washes between pretreatments.

Article Title: Tau Accumulation, Altered Phosphorylation, and Missorting Promote Neurodegeneration in Glaucoma
Article Snippet: Paragraph title: Retina and optic nerve immunohistochemistry. ... Some eyes were embedded in paraffin for the generation of thin retinal cross sections (4 μm) using a microtome (Leica Biosystems).

Software:

Article Title: Spondias purpurea L. (Anacardiaceae): Antioxidant and Antiulcer Activities of the Leaf Hexane Extract
Article Snippet: Ultrathin sections (4 μ m) were obtained using a microtome (model RM 2245, Leica Biosystems GmbH, Nussloch, Germany) and subjected to hematoxylin and eosin (HE) staining. .. Two slides were prepared for each animal, and all of them were examined under a light microscope (model DM500, Leica Biosystems GmbH, Nussloch, Germany), photographed using Leica camera (model EC3, Leica Biosystems GmbH, Nussloch, Germany) attached to the microscope, and then histopathologically analyzed using Leica Application Suite (LAS) EZ microscope software (Leica Biosystems GmbH, Nussloch, Germany) [ , ].

Article Title: Comparative Study on Pulmonary Toxicity in Mice Induced by Exposure to Unflavoured and Apple- and Strawberry-Flavoured Tobacco Waterpipe Smoke
Article Snippet: Sections of 5 μ m (7 sections per lung) were cut with a microtome (RM2125 RTS, Leica Biosystems, Nussloch, Germany) and stained with hematoxylin and eosin [ , ]. .. The morphometric analysis of neutrophil polymorphs and macrophages in lung tissues was carried out using ImageJ software ( http://rsbweb.nih.gov/ij/ ).

Chloramphenicol Acetyltransferase Assay:

Article Title: Interleukin-1β signaling in fenestrated capillaries is sufficient to trigger sickness responses in mice
Article Snippet: Immunohistochemistry (IHC) Cryopreserved frozen brains were sectioned at 30 μm and collected in three serial sets on a sliding microtome (Leica Biosystems, Buffalo Grove, Illinois; SM2000R) equipped with a freezing stage (Physitemp Instruments, Clifton, New Jersey; BFS-5MP). .. Brain sections were first washed with three changes of PBS then pretreated by quenching with 1% glycine (Sigma-Aldrich, CatG8898) in PBS and de-fixing in 0.05% sodium borohydride (Sigma-Aldrich, Cat#71321) in PBS, with three PBS washes between pretreatments.

Next-Generation Sequencing:

Article Title: Interleukin-1β signaling in fenestrated capillaries is sufficient to trigger sickness responses in mice
Article Snippet: Immunohistochemistry (IHC) Cryopreserved frozen brains were sectioned at 30 μm and collected in three serial sets on a sliding microtome (Leica Biosystems, Buffalo Grove, Illinois; SM2000R) equipped with a freezing stage (Physitemp Instruments, Clifton, New Jersey; BFS-5MP). .. Sections were then blocked in 5% normal goat serum (NGS; Sigma-Aldrich) and 1% hydrogen peroxide in PBS containing 0.3% triton X-100 (Tx; Sigma-Aldrich, Cat#X100) for 30 min at room temperature.

Labeling:

Article Title: Tau Accumulation, Altered Phosphorylation, and Missorting Promote Neurodegeneration in Glaucoma
Article Snippet: Some eyes were embedded in paraffin for the generation of thin retinal cross sections (4 μm) using a microtome (Leica Biosystems). .. Fluorescent labeling was observed using an Axioskop 2 Plus Microscope (Carl Zeiss) or a confocal microscope (Leica Microsystems).

Mouse Assay:

Article Title: Hochu-Ekki-To Improves Motor Function in an Amyotrophic Lateral Sclerosis Animal Model
Article Snippet: For hematoxylin and eosin (H & E) staining, the gastrocnemius muscle of the mice was fixed in 4% paraformaldehyde at 4 °C before embedding in paraffin. .. The tissues were cut into transverse sections (5 μm thick) using a microtome (Leica biosystems, IL, USA) and mounted on glass slides.

Light Microscopy:

Article Title: Spondias purpurea L. (Anacardiaceae): Antioxidant and Antiulcer Activities of the Leaf Hexane Extract
Article Snippet: Ultrathin sections (4 μ m) were obtained using a microtome (model RM 2245, Leica Biosystems GmbH, Nussloch, Germany) and subjected to hematoxylin and eosin (HE) staining. .. Two slides were prepared for each animal, and all of them were examined under a light microscope (model DM500, Leica Biosystems GmbH, Nussloch, Germany), photographed using Leica camera (model EC3, Leica Biosystems GmbH, Nussloch, Germany) attached to the microscope, and then histopathologically analyzed using Leica Application Suite (LAS) EZ microscope software (Leica Biosystems GmbH, Nussloch, Germany) [ , ].

Article Title: Isoalantolactone suppresses LPS-induced inflammation by inhibiting TRAF6 ubiquitination and alleviates acute lung injury
Article Snippet: The fixed lung was dehydrated, embedded in paraffin and then sectioned into 5 μm slicesusing a microtome (RM2235; Leica Biosystems, Wetzlar, Germany). .. Pathological changes were observed using a light microscope (RX51; Olympus Optical Co. Ltd, Tokyo, Japan) at ×200 magnification after hematoxylin and eosin (H & E) staining.

Article Title: Comparative Study on Pulmonary Toxicity in Mice Induced by Exposure to Unflavoured and Apple- and Strawberry-Flavoured Tobacco Waterpipe Smoke
Article Snippet: Sections of 5 μ m (7 sections per lung) were cut with a microtome (RM2125 RTS, Leica Biosystems, Nussloch, Germany) and stained with hematoxylin and eosin [ , ]. .. The stained sections were mounted on slides and evaluated blindly under a light microscope with a 40× objective lens by the histopathologist (SA) who contributed in this study.

Article Title: Anti-obesity and fatty liver-preventing activities of Lonicera caerulea in high-fat diet-fed mice
Article Snippet: Following embedding of the organs in paraffin using an automated tissue processor (Shandon Citadel 2000; Thermo Fisher Scientific, Inc.) and embedding center (Shandon Histocentre 3; Thermo Fisher Scientific, Inc.), 3-4-µ m serial sections were prepared using a microtome (RM2255; Leica Biosystems, Wetzlar, Germany). .. Representative tissue sections were stained with hematoxylin and eosin (H & E) and examined under a light microscope (Eclipse 80i; Nikon Corporation, Tokyo, Japan).

Concentration Assay:

Article Title: Spondias purpurea L. (Anacardiaceae): Antioxidant and Antiulcer Activities of the Leaf Hexane Extract
Article Snippet: Immediately, they were washed in buffer, dehydrated in an increasing alcohol concentration series, and fixed in historesin glycol methacrylate (Historesin Leica, Leica Biosystems GmbH, Nussloch, Germany). .. Ultrathin sections (4 μ m) were obtained using a microtome (model RM 2245, Leica Biosystems GmbH, Nussloch, Germany) and subjected to hematoxylin and eosin (HE) staining.

Incubation:

Article Title: Interleukin-1β signaling in fenestrated capillaries is sufficient to trigger sickness responses in mice
Article Snippet: Immunohistochemistry (IHC) Cryopreserved frozen brains were sectioned at 30 μm and collected in three serial sets on a sliding microtome (Leica Biosystems, Buffalo Grove, Illinois; SM2000R) equipped with a freezing stage (Physitemp Instruments, Clifton, New Jersey; BFS-5MP). .. Sections were incubated with primary antibodies overnight shaking at 4 °C in PBS containing 1% bovine serum albumin (Sigma-Aldrich, Cat#A2153) and 0.3% Tx.

Article Title: DNAJC14 Ameliorates Inner Ear Degeneration in the DFNB4 Mouse Model
Article Snippet: The paraffin blocks were sliced into 5-μm-thick sections in the midmodiolar plane using a microtome (Leica Biosystems, Nußloch, Germany). .. After incubation in tris-sodium citrate at 95°C for antigen retrieval, the tissues were blocked with 10% donkey serum and incubated with target-specific primary and secondary antibodies at 4°C overnight.

Article Title: Tau Accumulation, Altered Phosphorylation, and Missorting Promote Neurodegeneration in Glaucoma
Article Snippet: Some eyes were embedded in paraffin for the generation of thin retinal cross sections (4 μm) using a microtome (Leica Biosystems). .. The following primary antibodies were added to retinal or optic nerve sections in blocking solution and incubated overnight at 4°C, as described previously ( ; ): total tau (2–10 μg/ml; K9JA, Dako); total tau (1:1000; Tau 46, New England BioLabs); choline acetyltransferase (ChAT; 1 μg/ml; Millipore); calbindin (1:1000; Swant); protein kinase C α (PKCα; 3 μg/ml; Enzo Life Sciences); neurofilament H (NF-H; 10 μg/ml; Sternberger Monoclonals); and/or tubulin isoform βIII (TUJ1; 2.5 μg/ml; Sigma-Aldrich).

Confocal Laser Scanning Microscopy:

Article Title: Evaluation of dentinal tubule occlusion and depth of penetration of nano-hydroxyapatite derived from chicken eggshell powder with and without addition of sodium fluoride: An in vitro study
Article Snippet: .. The remaining ten discs of the experimental groups were sectioned longitudinally in the center using a microtome (Leica SP1600, Leica Biosystems Nussloch GmbH, Germany) to evaluate the depth of penetration using CLSM (LSM 510 META, Carl Zeiss MicroImaging GmbH, Jena, Germany). ..

Microscopy:

Article Title: Spondias purpurea L. (Anacardiaceae): Antioxidant and Antiulcer Activities of the Leaf Hexane Extract
Article Snippet: Ultrathin sections (4 μ m) were obtained using a microtome (model RM 2245, Leica Biosystems GmbH, Nussloch, Germany) and subjected to hematoxylin and eosin (HE) staining. .. Two slides were prepared for each animal, and all of them were examined under a light microscope (model DM500, Leica Biosystems GmbH, Nussloch, Germany), photographed using Leica camera (model EC3, Leica Biosystems GmbH, Nussloch, Germany) attached to the microscope, and then histopathologically analyzed using Leica Application Suite (LAS) EZ microscope software (Leica Biosystems GmbH, Nussloch, Germany) [ , ].

Article Title: Epigallocatechin Gallate-Modified Gelatins with Different Compositions Alter the Quality of Regenerated Bones
Article Snippet: Thin sections (6 μm in thickness) were prepared by using microtome (RM2255, Leica Biosystems, Wetzlar, Germany) and stained with Villanueva Goldner stain for the V.Goldner staining. .. Stained sections were captured by using a BZ-9000 digital microscope.

Article Title: Histological and Immunofluorescent Analysis of a Large Tributary of the Great Saphenous Vein Treated with a 1920 nm Endovenous Laser: Preliminary Findings
Article Snippet: A microtome (Reichert 2040 Microtome, Leica Biosystems, Milton Keynes, UK) was then used to cut 5 μm sections and these were placed onto Superfrost slides. .. Images were taken at ×40, ×100, and ×200 magnifications using an EVOS microscope (Electron Microscopy Sciences, Hatfield, PA, USA).

Article Title: DNAJC14 Ameliorates Inner Ear Degeneration in the DFNB4 Mouse Model
Article Snippet: The paraffin blocks were sliced into 5-μm-thick sections in the midmodiolar plane using a microtome (Leica Biosystems, Nußloch, Germany). .. The samples were then mounted with mounting solution (Sigma-Aldrich) and viewed under an LSM780 confocal microscope (Zeiss, Jena, Germany).

Article Title: Tau Accumulation, Altered Phosphorylation, and Missorting Promote Neurodegeneration in Glaucoma
Article Snippet: Some eyes were embedded in paraffin for the generation of thin retinal cross sections (4 μm) using a microtome (Leica Biosystems). .. Fluorescent labeling was observed using an Axioskop 2 Plus Microscope (Carl Zeiss) or a confocal microscope (Leica Microsystems).

Article Title: Genetic Screening for Mutants with Altered Seminal Root Numbers in Hexaploid Wheat Using a High-Throughput Root Phenotyping Platform
Article Snippet: Then, embryos were cleared in xylene, embedded in paraffin and sectioned (5 μM) using a microtome (Leica Biosystems, Germany). .. A stereo microscope (SZX16, Olympus, Tokyo, Japan) was used for imaging.

Staining:

Article Title: Spondias purpurea L. (Anacardiaceae): Antioxidant and Antiulcer Activities of the Leaf Hexane Extract
Article Snippet: .. Ultrathin sections (4 μ m) were obtained using a microtome (model RM 2245, Leica Biosystems GmbH, Nussloch, Germany) and subjected to hematoxylin and eosin (HE) staining. .. Two slides were prepared for each animal, and all of them were examined under a light microscope (model DM500, Leica Biosystems GmbH, Nussloch, Germany), photographed using Leica camera (model EC3, Leica Biosystems GmbH, Nussloch, Germany) attached to the microscope, and then histopathologically analyzed using Leica Application Suite (LAS) EZ microscope software (Leica Biosystems GmbH, Nussloch, Germany) [ , ].

Article Title: Epigallocatechin Gallate-Modified Gelatins with Different Compositions Alter the Quality of Regenerated Bones
Article Snippet: .. Thin sections (6 μm in thickness) were prepared by using microtome (RM2255, Leica Biosystems, Wetzlar, Germany) and stained with Villanueva Goldner stain for the V.Goldner staining. .. Stained sections were captured by using a BZ-9000 digital microscope.

Article Title: Isoalantolactone suppresses LPS-induced inflammation by inhibiting TRAF6 ubiquitination and alleviates acute lung injury
Article Snippet: The fixed lung was dehydrated, embedded in paraffin and then sectioned into 5 μm slicesusing a microtome (RM2235; Leica Biosystems, Wetzlar, Germany). .. Pathological changes were observed using a light microscope (RX51; Olympus Optical Co. Ltd, Tokyo, Japan) at ×200 magnification after hematoxylin and eosin (H & E) staining.

Article Title: Comparative Study on Pulmonary Toxicity in Mice Induced by Exposure to Unflavoured and Apple- and Strawberry-Flavoured Tobacco Waterpipe Smoke
Article Snippet: .. Sections of 5 μ m (7 sections per lung) were cut with a microtome (RM2125 RTS, Leica Biosystems, Nussloch, Germany) and stained with hematoxylin and eosin [ , ]. .. The stained sections were mounted on slides and evaluated blindly under a light microscope with a 40× objective lens by the histopathologist (SA) who contributed in this study.

Article Title: Anti-obesity and fatty liver-preventing activities of Lonicera caerulea in high-fat diet-fed mice
Article Snippet: Following embedding of the organs in paraffin using an automated tissue processor (Shandon Citadel 2000; Thermo Fisher Scientific, Inc.) and embedding center (Shandon Histocentre 3; Thermo Fisher Scientific, Inc.), 3-4-µ m serial sections were prepared using a microtome (RM2255; Leica Biosystems, Wetzlar, Germany). .. Representative tissue sections were stained with hematoxylin and eosin (H & E) and examined under a light microscope (Eclipse 80i; Nikon Corporation, Tokyo, Japan).

Article Title: Histological and Immunofluorescent Analysis of a Large Tributary of the Great Saphenous Vein Treated with a 1920 nm Endovenous Laser: Preliminary Findings
Article Snippet: A microtome (Reichert 2040 Microtome, Leica Biosystems, Milton Keynes, UK) was then used to cut 5 μm sections and these were placed onto Superfrost slides. .. The slides were stained with haematoxylin and eosin (H & E) and Martius Scarlet Blue (MSB).

Article Title: DNAJC14 Ameliorates Inner Ear Degeneration in the DFNB4 Mouse Model
Article Snippet: For paraffin sectioning and H & E staining, serial dehydration of the tissues was performed with ethanol and xylene. .. The paraffin blocks were sliced into 5-μm-thick sections in the midmodiolar plane using a microtome (Leica Biosystems, Nußloch, Germany).

Article Title: Genetic Screening for Mutants with Altered Seminal Root Numbers in Hexaploid Wheat Using a High-Throughput Root Phenotyping Platform
Article Snippet: Then, embryos were cleared in xylene, embedded in paraffin and sectioned (5 μM) using a microtome (Leica Biosystems, Germany). .. Cross sections were de-paraffinized with histoclear, rehydrated and stained with Harris Hematoxylin.

Article Title: Nutritional secondary hyperparathyroidism in two ponies
Article Snippet: Fifteen-µm serial sections were cut in the frontal plane using a circular diamond-coated Leica SP1600 saw microtome (Leica Biosystems), polished under running water, and air dried for 24 hours in an incubator at 37°C. .. Nondecalcified sections were stained using 1% toluidine blue (pH 3.7).

Article Title: Hochu-Ekki-To Improves Motor Function in an Amyotrophic Lateral Sclerosis Animal Model
Article Snippet: For hematoxylin and eosin (H & E) staining, the gastrocnemius muscle of the mice was fixed in 4% paraformaldehyde at 4 °C before embedding in paraffin. .. The tissues were cut into transverse sections (5 μm thick) using a microtome (Leica biosystems, IL, USA) and mounted on glass slides.

Activated Clotting Time Assay:

Article Title: Histological and Immunofluorescent Analysis of a Large Tributary of the Great Saphenous Vein Treated with a 1920 nm Endovenous Laser: Preliminary Findings
Article Snippet: One section was untreated, to act as a control, and the other four were treated with the 1920 nm EVL at 4W, with four different pull back speeds; 5, 10, 15, and 20 s/cm. .. A microtome (Reichert 2040 Microtome, Leica Biosystems, Milton Keynes, UK) was then used to cut 5 μm sections and these were placed onto Superfrost slides.

other:

Article Title: Effects of Curcumin (Curcuma longa) on Learning and Spatial Memory as Well as Cell Proliferation and Neuroblast Differentiation in Adult and Aged Mice by Upregulating Brain-Derived Neurotrophic Factor and CREB Signaling
Article Snippet: Sections 3 μm thick were serially cut using a microtome (Leica, RM2245; Leica Biosystems, Newcastle upon Tyne, United Kingdom), and they were mounted onto silane-coated slides.

Immunofluorescence:

Article Title: DNAJC14 Ameliorates Inner Ear Degeneration in the DFNB4 Mouse Model
Article Snippet: Paragraph title: Whole-Mount Immunofluorescence ... The paraffin blocks were sliced into 5-μm-thick sections in the midmodiolar plane using a microtome (Leica Biosystems, Nußloch, Germany).

Blocking Assay:

Article Title: Tau Accumulation, Altered Phosphorylation, and Missorting Promote Neurodegeneration in Glaucoma
Article Snippet: Some eyes were embedded in paraffin for the generation of thin retinal cross sections (4 μm) using a microtome (Leica Biosystems). .. The following primary antibodies were added to retinal or optic nerve sections in blocking solution and incubated overnight at 4°C, as described previously ( ; ): total tau (2–10 μg/ml; K9JA, Dako); total tau (1:1000; Tau 46, New England BioLabs); choline acetyltransferase (ChAT; 1 μg/ml; Millipore); calbindin (1:1000; Swant); protein kinase C α (PKCα; 3 μg/ml; Enzo Life Sciences); neurofilament H (NF-H; 10 μg/ml; Sternberger Monoclonals); and/or tubulin isoform βIII (TUJ1; 2.5 μg/ml; Sigma-Aldrich).

Imaging:

Article Title: Epigallocatechin Gallate-Modified Gelatins with Different Compositions Alter the Quality of Regenerated Bones
Article Snippet: Paragraph title: 4.7. V.Goldner Staining and FTIR Imaging ... Thin sections (6 μm in thickness) were prepared by using microtome (RM2255, Leica Biosystems, Wetzlar, Germany) and stained with Villanueva Goldner stain for the V.Goldner staining.

Article Title: Genetic Screening for Mutants with Altered Seminal Root Numbers in Hexaploid Wheat Using a High-Throughput Root Phenotyping Platform
Article Snippet: Then, embryos were cleared in xylene, embedded in paraffin and sectioned (5 μM) using a microtome (Leica Biosystems, Germany). .. A stereo microscope (SZX16, Olympus, Tokyo, Japan) was used for imaging.

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    Leica Biosystems leica vt1200s vibrating blade microtome
    A. <t>Leica</t> <t>VT1200S</t> vibrating blade microtome with stage pre-chilled with ice. B. Two transverse slices (300 μm) that were cut using the microtome, and the remaining tissue block.
    Leica Vt1200s Vibrating Blade Microtome, supplied by Leica Biosystems, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/leica vt1200s vibrating blade microtome/product/Leica Biosystems
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    leica vt1200s vibrating blade microtome - by Bioz Stars, 2020-03
    86/100 stars
      Buy from Supplier

    99
    Leica Biosystems microtome
    A. <t>Leica</t> <t>VT1200S</t> vibrating blade microtome with stage pre-chilled with ice. B. Two transverse slices (300 μm) that were cut using the microtome, and the remaining tissue block.
    Microtome, supplied by Leica Biosystems, used in various techniques. Bioz Stars score: 99/100, based on 62 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/microtome/product/Leica Biosystems
    Average 99 stars, based on 62 article reviews
    Price from $9.99 to $1999.99
    microtome - by Bioz Stars, 2020-03
    99/100 stars
      Buy from Supplier

    Image Search Results


    A. Leica VT1200S vibrating blade microtome with stage pre-chilled with ice. B. Two transverse slices (300 μm) that were cut using the microtome, and the remaining tissue block.

    Journal: Bio-protocol

    Article Title: Measurement of Inositol Triphosphate Levels from Rat Hippocampal Slices

    doi:

    Figure Lengend Snippet: A. Leica VT1200S vibrating blade microtome with stage pre-chilled with ice. B. Two transverse slices (300 μm) that were cut using the microtome, and the remaining tissue block.

    Article Snippet: Scissors (Fine science tools, catalog numbers: 14000-12 and 14060-10) Forceps (Stoelting, catalog numbers: 52104-37 and 52104-35) Hemostat (Fine science tools, catalog number: 13008-12) Spatula (Thermo Fisher Scientific, catalog number: ) Accuspin micro 17R microcentrifuge (Thermo Fisher Scientific, catalog number: 13-100-676) Biophotometer (Eppendorf AG, catalog number: 6133000010) Leica VT1200S vibrating blade microtome (Leica Biosystems Nussloch GmbH, catalog number: 1491200S001) Isotemp 105 water bath (Thermo Fisher Scientific, catalog number: S63077Q) Qsonica Q500 sonicator (Thermo Fisher Scientific, catalog number: 15-338-282) Synergy 4 multimode microplate reader (BioTek Instruments) Vapor pressure osmometer 5520 (Wescor) Accumet AB15 plus pH meter (Thermo Fisher Scientific, catalog number: 13-636-AB15PC) Rotator (Labline Instruments) Micropipettes (2–20 μl, 20–200 μl and 100–1,000 μl) (Capitol Scientific, Eppendorf, catalog numbers: 3120000038, 3120000054 and 3120000062) Glass plate (Electron Microscopy Sciences, catalog number: 70312-22) Glass staining dish (Newcomer supply, catalog number: 6816A) Coarse fritted glass bubbler (Corning, catalog number: 39533-12EC)

    Techniques: Blocking Assay

    A. Perfused rat brain on a glass plate on ice. The dashed lines indicate where cuts are made and numbers indicate the order of the cuts. B. Blocked brain with the two hemispheres separated. C. One hemisphere of the brain mounted on the mounting disk of a Leica VT1200S vibrating microtome.

    Journal: Bio-protocol

    Article Title: Measurement of Inositol Triphosphate Levels from Rat Hippocampal Slices

    doi:

    Figure Lengend Snippet: A. Perfused rat brain on a glass plate on ice. The dashed lines indicate where cuts are made and numbers indicate the order of the cuts. B. Blocked brain with the two hemispheres separated. C. One hemisphere of the brain mounted on the mounting disk of a Leica VT1200S vibrating microtome.

    Article Snippet: Scissors (Fine science tools, catalog numbers: 14000-12 and 14060-10) Forceps (Stoelting, catalog numbers: 52104-37 and 52104-35) Hemostat (Fine science tools, catalog number: 13008-12) Spatula (Thermo Fisher Scientific, catalog number: ) Accuspin micro 17R microcentrifuge (Thermo Fisher Scientific, catalog number: 13-100-676) Biophotometer (Eppendorf AG, catalog number: 6133000010) Leica VT1200S vibrating blade microtome (Leica Biosystems Nussloch GmbH, catalog number: 1491200S001) Isotemp 105 water bath (Thermo Fisher Scientific, catalog number: S63077Q) Qsonica Q500 sonicator (Thermo Fisher Scientific, catalog number: 15-338-282) Synergy 4 multimode microplate reader (BioTek Instruments) Vapor pressure osmometer 5520 (Wescor) Accumet AB15 plus pH meter (Thermo Fisher Scientific, catalog number: 13-636-AB15PC) Rotator (Labline Instruments) Micropipettes (2–20 μl, 20–200 μl and 100–1,000 μl) (Capitol Scientific, Eppendorf, catalog numbers: 3120000038, 3120000054 and 3120000062) Glass plate (Electron Microscopy Sciences, catalog number: 70312-22) Glass staining dish (Newcomer supply, catalog number: 6816A) Coarse fritted glass bubbler (Corning, catalog number: 39533-12EC)

    Techniques: