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Carl Zeiss microtome
Microtome, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/microtome/product/Carl Zeiss
Average 93 stars, based on 7 article reviews
Price from $9.99 to $1999.99
microtome - by Bioz Stars, 2020-04
93/100 stars

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Related Articles

Electron Microscopy:

Article Title: Expression of Matrix Metalloprotease-2-Cleaved Laminin-5 in Breast Remodeling Stimulated by Sex Steroids
Article Snippet: .. Mammary gland explants were either paraffin-embedded, sectioned, and analyzed by scanning electron microscopy (stereoscan 360 scanning electron microscope at an accelerating voltage of 10 kV) or snap-frozen in liquid nitrogen, embedded in OCT compound (Miles Laboratories Inc., Naperville, IL), cut into 5-μm sections with a microtome (model HM 505E, Carl Zeiss, Oberkochen, Germany), and stained with antibodies. .. Whole-mount staining of mammary glands was performed as described.

Immunohistochemistry:

Article Title: Characterization and in ovo vascularization of a 3D-printed hydroxyapatite scaffold with different extracellular matrix coatings under perfusion culture
Article Snippet: Paragraph title: Immunohistochemistry and histological staining ... After washing in 1× PBS, samples were paraffin-embedded (TPC 15DUO , Medite TBS88 Paraffin embedding system cool unit, Switzerland) and sectioned (7 µm thick) by means of a microtome (Zeiss HYRAX M55).

Article Title: Expression of Matrix Metalloprotease-2-Cleaved Laminin-5 in Breast Remodeling Stimulated by Sex Steroids
Article Snippet: Paragraph title: Electron Scanning Microscopy, Histology, and Immunohistochemistry ... Mammary gland explants were either paraffin-embedded, sectioned, and analyzed by scanning electron microscopy (stereoscan 360 scanning electron microscope at an accelerating voltage of 10 kV) or snap-frozen in liquid nitrogen, embedded in OCT compound (Miles Laboratories Inc., Naperville, IL), cut into 5-μm sections with a microtome (model HM 505E, Carl Zeiss, Oberkochen, Germany), and stained with antibodies.

Article Title: Lamin-B1 is a senescence-associated biomarker in clear-cell renal cell carcinoma
Article Snippet: Paragraph title: Immunohistochemistry ... In the next step tissues were cut into 2 µm thick slices using a microtome (Hyrax M55; Carl Zeiss AG).

Inverted Microscopy:

Article Title: Characterization and in ovo vascularization of a 3D-printed hydroxyapatite scaffold with different extracellular matrix coatings under perfusion culture
Article Snippet: After washing in 1× PBS, samples were paraffin-embedded (TPC 15DUO , Medite TBS88 Paraffin embedding system cool unit, Switzerland) and sectioned (7 µm thick) by means of a microtome (Zeiss HYRAX M55). .. Histology samples were observed under an Olympus CKX41 inverted microscope (n =4 for each condition).

Electroporation:

Article Title: A genetically-encoded chloride and pH sensor for dissociating ion dynamics in the nervous system
Article Snippet: Following electroporation the uterine horns were replaced and the dam allowed to recover and litter as normal. .. Coronal cortical slices (350–400 μm thickness) were cut using a vibrating microtome (Microm HM650V, Carl Zeiss Ltd) and slices were maintained in an interface chamber between humidified carbogen gas (95% O2 , 5% CO2 ) and ACSF (at 20–25°C).

Software:

Article Title: Effects of Polylactide Copolymer Implants and Platelet-Rich Plasma on Bone Regeneration within a Large Calvarial Defect in Sheep
Article Snippet: After decalcification, slices were rinsed with 70% ethanol and cut into 5 μ m sections using a microtome (Hyrax M55, Zeiss, Germany). .. The specimens were examined at 200x under an Olympus BX53 Digital Upright Microscope (Olympus Deutschland GmbH, Hamburg, Germany) and representative digital images were analyzed by Image-Pro Plus 6.0 software (Media Cybernetics, Inc., Rockville, USA).

Transferring:

Article Title: A genetically-encoded chloride and pH sensor for dissociating ion dynamics in the nervous system
Article Snippet: Each embryo was injected through the uterine wall with 0.5–1 ul ClopHensorN plasmid (2 ug/ul) in PBS with 0.03% fast green (Sigma) using a thin-walled glass pipette (WPI) pulled to a ~50 μm tip. .. Coronal cortical slices (350–400 μm thickness) were cut using a vibrating microtome (Microm HM650V, Carl Zeiss Ltd) and slices were maintained in an interface chamber between humidified carbogen gas (95% O2 , 5% CO2 ) and ACSF (at 20–25°C).

Transfection:

Article Title: A genetically-encoded chloride and pH sensor for dissociating ion dynamics in the nervous system
Article Snippet: Paragraph title: Slice preparation and DNA transfection ... Coronal cortical slices (350–400 μm thickness) were cut using a vibrating microtome (Microm HM650V, Carl Zeiss Ltd) and slices were maintained in an interface chamber between humidified carbogen gas (95% O2 , 5% CO2 ) and ACSF (at 20–25°C).

Blocking Assay:

Article Title: Lamin-B1 is a senescence-associated biomarker in clear-cell renal cell carcinoma
Article Snippet: In the next step tissues were cut into 2 µm thick slices using a microtome (Hyrax M55; Carl Zeiss AG). .. Briefly, following heat-induced antigen retrieval, which was comprised of initial heating at 94–98°C for 5 min, slides were washed with the kit's washing buffer, rehydrated in descending alcohol series with ethanol concentrations ranging from 50–100% and xylene, and blocked with the kit's blocking solution (Envision Flex Peroxidase-Blocking Reagent) at room temperature for 5 min. Tissue microarray slides were stained with a polyclonal anti-LMNB1 rabbit antiserum (1:1,000) for 30 min at room temperature (Abcam; cat. no. ab16048).

Article Title: Imaging Calcium Responses in GFP-tagged Neurons of Hypothalamic Mouse Brain Slices
Article Snippet: Glue the brain with the cut section on the baseplate of the microtome (Zeiss, Hyrax V50) using low amounts of a fast curing high performance cyanoacrylate adhesive superglue (Loctite 406; Figure 1A ). .. In addition, glue a 1 cm3 agar gel block (see 1.5) at the ventral side of the brain (see 'v' in Figure 2B ) onto the baseplate of the microtome (Zeiss, Hyrax V50) to support and fix the brain while slicing.

Mouse Assay:

Article Title: Neuroprotection, Recovery of Function and Endogenous Neurogenesis in Traumatic Spinal Cord Injury Following Transplantation of Activated Adipose Tissue
Article Snippet: The cords were then sectioned by means of a microtome (Zeiss, Oberkochen, Germany) cutting sections 18 µm in thickness. .. This analysis was performed on 3 animals per experimental group: 1) lesioned mice, 2) lesioned mice transplanted with LS, and 3) lesioned mice transplanted with MALS.

Article Title: Loss of Tenomodulin Results in Reduced Self-Renewal and Augmented Senescence of Tendon Stem/Progenitor Cells
Article Snippet: .. Detection of p16, p21, and p53 on tendon tissue sections Paraffin Achilles tendon sections (7 μm thick) from 6-month-old mice were collected with Microtome (Hyrax M55; Carl Zeiss) and treated with 0.2% hyaluronidase/PBS for 30 min. After washing, the sections were blocked with the Mouse-on-Mouse kit (Vector Laboratories) and then incubated with primary mouse antibodies against p16 (Santa Cruz Biotechnology), p21 (BD Biosciences), and p53 (Abcam) overnight at 4°C. .. Secondary Alexa Flour 546 antibody (Life Technology) was applied for 1 h at room temperature, followed by DAPI staining for 5 min. Fluorescent images were taken with the Axiocam MRm camera on the Observer Z1 microscope (Carl Zeiss).

Article Title: A genetically-encoded chloride and pH sensor for dissociating ion dynamics in the nervous system
Article Snippet: At postnatal day 21 the mice were killed and the brain rapidly removed and placed in ice-cold (0 to +4°C) artificial cerebro-spinal fluid (ACSF), bubbled with 95% O2 /5% CO2 . .. Coronal cortical slices (350–400 μm thickness) were cut using a vibrating microtome (Microm HM650V, Carl Zeiss Ltd) and slices were maintained in an interface chamber between humidified carbogen gas (95% O2 , 5% CO2 ) and ACSF (at 20–25°C).

Light Microscopy:

Article Title: The Vacuolar Manganese Transporter MTP8 Determines Tolerance to Iron Deficiency-Induced Chlorosis in Arabidopsis 1The Vacuolar Manganese Transporter MTP8 Determines Tolerance to Iron Deficiency-Induced Chlorosis in Arabidopsis 1 [OPEN]
Article Snippet: .. Five-micrometer cross sections of stained roots were obtained by cutting the fixed and resin-embedded samples using a microtome (Ultracut; Leica) and observed with a light microscope (Axioskop; Carl Zeiss). .. Chlorophyll concentrations were determined by extracting shoot samples with spectrophotometric grade N , N ′-dimethyl formamide (Sigma-Aldrich) at 4°C for 48 h. The absorbance at 647 and 664 nm was measured in extracts according to .

Microarray:

Article Title: Lamin-B1 is a senescence-associated biomarker in clear-cell renal cell carcinoma
Article Snippet: In the next step tissues were cut into 2 µm thick slices using a microtome (Hyrax M55; Carl Zeiss AG). .. Briefly, following heat-induced antigen retrieval, which was comprised of initial heating at 94–98°C for 5 min, slides were washed with the kit's washing buffer, rehydrated in descending alcohol series with ethanol concentrations ranging from 50–100% and xylene, and blocked with the kit's blocking solution (Envision Flex Peroxidase-Blocking Reagent) at room temperature for 5 min. Tissue microarray slides were stained with a polyclonal anti-LMNB1 rabbit antiserum (1:1,000) for 30 min at room temperature (Abcam; cat. no. ab16048).

Incubation:

Article Title: Characterization and in ovo vascularization of a 3D-printed hydroxyapatite scaffold with different extracellular matrix coatings under perfusion culture
Article Snippet: After fixation, samples were decalcified by incubation in a solution with 7 % (w/v) EDTA (7×104 µg/ml) and 10% (w/v) sucrose (105 µg/ml) at 37°C, 5% CO2 on an orbital shaker for 8-10 days. .. After washing in 1× PBS, samples were paraffin-embedded (TPC 15DUO , Medite TBS88 Paraffin embedding system cool unit, Switzerland) and sectioned (7 µm thick) by means of a microtome (Zeiss HYRAX M55).

Article Title: Loss of Tenomodulin Results in Reduced Self-Renewal and Augmented Senescence of Tendon Stem/Progenitor Cells
Article Snippet: .. Detection of p16, p21, and p53 on tendon tissue sections Paraffin Achilles tendon sections (7 μm thick) from 6-month-old mice were collected with Microtome (Hyrax M55; Carl Zeiss) and treated with 0.2% hyaluronidase/PBS for 30 min. After washing, the sections were blocked with the Mouse-on-Mouse kit (Vector Laboratories) and then incubated with primary mouse antibodies against p16 (Santa Cruz Biotechnology), p21 (BD Biosciences), and p53 (Abcam) overnight at 4°C. .. Secondary Alexa Flour 546 antibody (Life Technology) was applied for 1 h at room temperature, followed by DAPI staining for 5 min. Fluorescent images were taken with the Axiocam MRm camera on the Observer Z1 microscope (Carl Zeiss).

Article Title: Lamin-B1 is a senescence-associated biomarker in clear-cell renal cell carcinoma
Article Snippet: In the next step tissues were cut into 2 µm thick slices using a microtome (Hyrax M55; Carl Zeiss AG). .. For Ki-67, sections were incubated with Flex monoclonal Mouse anti-human Ki-67 Clone MIB-1 (dilution: ready to use solution; EnVision FLEX; Agilent Technologies, Inc.; cat. no. IR626) at room temperature for 20 min according to the manufacturer's protocol.

Article Title: Cell-specific expression of tryptophan decarboxylase and 10-hydroxygeraniol oxidoreductase, key genes involved in camptothecin biosynthesis in Camptotheca acuminata Decne (Nyssaceae)
Article Snippet: The paraffin-embedded samples were sectioned (8-10 μm) using a microtome (Zeiss). .. The sections were then briefly rinsed in 0.05 M Tris/HCl, pH 7.6, and incubated with 0.5 ml of proteinase K (1 μg ml-1 ) in 0.05 M Tris-HCl, pH 7.6, for 25 minutes at 37°C.

Article Title: The Vacuolar Manganese Transporter MTP8 Determines Tolerance to Iron Deficiency-Induced Chlorosis in Arabidopsis 1The Vacuolar Manganese Transporter MTP8 Determines Tolerance to Iron Deficiency-Induced Chlorosis in Arabidopsis 1 [OPEN]
Article Snippet: Samples were incubated at 37°C in a GUS reaction buffer containing 0.4 mg mL−1 5-bromo-4-chloro-3-indolyl-β- d -glucuronide, 50 m m sodium phosphate (pH 7.2), and 0.5 m m ferrocyanide. .. Five-micrometer cross sections of stained roots were obtained by cutting the fixed and resin-embedded samples using a microtome (Ultracut; Leica) and observed with a light microscope (Axioskop; Carl Zeiss).

Microscopy:

Article Title: Characterization and in ovo vascularization of a 3D-printed hydroxyapatite scaffold with different extracellular matrix coatings under perfusion culture
Article Snippet: Pictures of entire scaffolds on a glass slide were taken using an Olympus Laser Confocal Scanning Microscope FV1000D spectral type. .. After washing in 1× PBS, samples were paraffin-embedded (TPC 15DUO , Medite TBS88 Paraffin embedding system cool unit, Switzerland) and sectioned (7 µm thick) by means of a microtome (Zeiss HYRAX M55).

Article Title: Expression of Matrix Metalloprotease-2-Cleaved Laminin-5 in Breast Remodeling Stimulated by Sex Steroids
Article Snippet: .. Mammary gland explants were either paraffin-embedded, sectioned, and analyzed by scanning electron microscopy (stereoscan 360 scanning electron microscope at an accelerating voltage of 10 kV) or snap-frozen in liquid nitrogen, embedded in OCT compound (Miles Laboratories Inc., Naperville, IL), cut into 5-μm sections with a microtome (model HM 505E, Carl Zeiss, Oberkochen, Germany), and stained with antibodies. .. Whole-mount staining of mammary glands was performed as described.

Article Title: Loss of Tenomodulin Results in Reduced Self-Renewal and Augmented Senescence of Tendon Stem/Progenitor Cells
Article Snippet: Detection of p16, p21, and p53 on tendon tissue sections Paraffin Achilles tendon sections (7 μm thick) from 6-month-old mice were collected with Microtome (Hyrax M55; Carl Zeiss) and treated with 0.2% hyaluronidase/PBS for 30 min. After washing, the sections were blocked with the Mouse-on-Mouse kit (Vector Laboratories) and then incubated with primary mouse antibodies against p16 (Santa Cruz Biotechnology), p21 (BD Biosciences), and p53 (Abcam) overnight at 4°C. .. Secondary Alexa Flour 546 antibody (Life Technology) was applied for 1 h at room temperature, followed by DAPI staining for 5 min. Fluorescent images were taken with the Axiocam MRm camera on the Observer Z1 microscope (Carl Zeiss).

Article Title: Effects of Polylactide Copolymer Implants and Platelet-Rich Plasma on Bone Regeneration within a Large Calvarial Defect in Sheep
Article Snippet: After decalcification, slices were rinsed with 70% ethanol and cut into 5 μ m sections using a microtome (Hyrax M55, Zeiss, Germany). .. The specimens were examined at 200x under an Olympus BX53 Digital Upright Microscope (Olympus Deutschland GmbH, Hamburg, Germany) and representative digital images were analyzed by Image-Pro Plus 6.0 software (Media Cybernetics, Inc., Rockville, USA).

Mass Spectrometry:

Article Title: A genetically-encoded chloride and pH sensor for dissociating ion dynamics in the nervous system
Article Snippet: Paddle electrodes (Nepagene, CUY650) were used to deliver five 50 ms, 42 V pulses at 1 Hz from a square pulse generator (BTX, ECM 830). .. Coronal cortical slices (350–400 μm thickness) were cut using a vibrating microtome (Microm HM650V, Carl Zeiss Ltd) and slices were maintained in an interface chamber between humidified carbogen gas (95% O2 , 5% CO2 ) and ACSF (at 20–25°C).

Sequencing:

Article Title: Chronic Multiple Sclerosis Lesions: Characterization with High-Field-Strength MR Imaging
Article Snippet: A three-dimensional multiecho gradient-echo sequence was performed with the following parameters: echo times, 8.7, 25.2, 41.7, and 58.2 msec; repetition time, 200 msec; spatial resolution, 0.2-mm isotropic; and flip angle = 20°. .. Small segments (2 × 4 × 3 mm3 each) were cut in 10-μm-thick serial slices with a microtome (Carl Zeiss MicroImaging, Thornwood, NY) and mounted onto Superfrost glass slides (Thermo Fisher Scientific, Waltham, Mass).

Staining:

Article Title: Characterization and in ovo vascularization of a 3D-printed hydroxyapatite scaffold with different extracellular matrix coatings under perfusion culture
Article Snippet: Paragraph title: Immunohistochemistry and histological staining ... After washing in 1× PBS, samples were paraffin-embedded (TPC 15DUO , Medite TBS88 Paraffin embedding system cool unit, Switzerland) and sectioned (7 µm thick) by means of a microtome (Zeiss HYRAX M55).

Article Title: Expression of Matrix Metalloprotease-2-Cleaved Laminin-5 in Breast Remodeling Stimulated by Sex Steroids
Article Snippet: .. Mammary gland explants were either paraffin-embedded, sectioned, and analyzed by scanning electron microscopy (stereoscan 360 scanning electron microscope at an accelerating voltage of 10 kV) or snap-frozen in liquid nitrogen, embedded in OCT compound (Miles Laboratories Inc., Naperville, IL), cut into 5-μm sections with a microtome (model HM 505E, Carl Zeiss, Oberkochen, Germany), and stained with antibodies. .. Whole-mount staining of mammary glands was performed as described.

Article Title: Loss of Tenomodulin Results in Reduced Self-Renewal and Augmented Senescence of Tendon Stem/Progenitor Cells
Article Snippet: Detection of p16, p21, and p53 on tendon tissue sections Paraffin Achilles tendon sections (7 μm thick) from 6-month-old mice were collected with Microtome (Hyrax M55; Carl Zeiss) and treated with 0.2% hyaluronidase/PBS for 30 min. After washing, the sections were blocked with the Mouse-on-Mouse kit (Vector Laboratories) and then incubated with primary mouse antibodies against p16 (Santa Cruz Biotechnology), p21 (BD Biosciences), and p53 (Abcam) overnight at 4°C. .. Secondary Alexa Flour 546 antibody (Life Technology) was applied for 1 h at room temperature, followed by DAPI staining for 5 min. Fluorescent images were taken with the Axiocam MRm camera on the Observer Z1 microscope (Carl Zeiss).

Article Title: Lamin-B1 is a senescence-associated biomarker in clear-cell renal cell carcinoma
Article Snippet: In the next step tissues were cut into 2 µm thick slices using a microtome (Hyrax M55; Carl Zeiss AG). .. Briefly, following heat-induced antigen retrieval, which was comprised of initial heating at 94–98°C for 5 min, slides were washed with the kit's washing buffer, rehydrated in descending alcohol series with ethanol concentrations ranging from 50–100% and xylene, and blocked with the kit's blocking solution (Envision Flex Peroxidase-Blocking Reagent) at room temperature for 5 min. Tissue microarray slides were stained with a polyclonal anti-LMNB1 rabbit antiserum (1:1,000) for 30 min at room temperature (Abcam; cat. no. ab16048).

Article Title: "Heavy Eye" Syndrome in the Absence of High Myopia: A Connective Tissue Degeneration in Elderly Strabismic Patients
Article Snippet: As previously described, the whole orbits were sectioned into 10 micron thick sections by a microtome (HM325, Carl-Zeiss, Thornwood, NY) after tissue processing, including formalin fixation, decalcification, dehydration, and embedding in paraffin ( ). .. Masson trichrome stain was used to differentially color collagen (blue) and muscle (red).

Article Title: Effects of Polylactide Copolymer Implants and Platelet-Rich Plasma on Bone Regeneration within a Large Calvarial Defect in Sheep
Article Snippet: After decalcification, slices were rinsed with 70% ethanol and cut into 5 μ m sections using a microtome (Hyrax M55, Zeiss, Germany). .. Microscopic specimens were stained with Masson's trichrome.

Article Title: Chronic Multiple Sclerosis Lesions: Characterization with High-Field-Strength MR Imaging
Article Snippet: Small segments (2 × 4 × 3 mm3 each) were cut in 10-μm-thick serial slices with a microtome (Carl Zeiss MicroImaging, Thornwood, NY) and mounted onto Superfrost glass slides (Thermo Fisher Scientific, Waltham, Mass). .. Perls staining for iron and standard Luxol fast blue staining for myelin, as well as immunochemical antibody staining for ferritin, were performed on consecutive slices, and the results were compared with MR images.

Article Title: The Vacuolar Manganese Transporter MTP8 Determines Tolerance to Iron Deficiency-Induced Chlorosis in Arabidopsis 1The Vacuolar Manganese Transporter MTP8 Determines Tolerance to Iron Deficiency-Induced Chlorosis in Arabidopsis 1 [OPEN]
Article Snippet: .. Five-micrometer cross sections of stained roots were obtained by cutting the fixed and resin-embedded samples using a microtome (Ultracut; Leica) and observed with a light microscope (Axioskop; Carl Zeiss). .. Chlorophyll concentrations were determined by extracting shoot samples with spectrophotometric grade N , N ′-dimethyl formamide (Sigma-Aldrich) at 4°C for 48 h. The absorbance at 647 and 664 nm was measured in extracts according to .

Injection:

Article Title: Neuroprotection, Recovery of Function and Endogenous Neurogenesis in Traumatic Spinal Cord Injury Following Transplantation of Activated Adipose Tissue
Article Snippet: The animals were then allowed to recover and perfused 10 days after the tracer injection. .. The cords were then sectioned by means of a microtome (Zeiss, Oberkochen, Germany) cutting sections 18 µm in thickness.

Article Title: A genetically-encoded chloride and pH sensor for dissociating ion dynamics in the nervous system
Article Snippet: Each embryo was injected through the uterine wall with 0.5–1 ul ClopHensorN plasmid (2 ug/ul) in PBS with 0.03% fast green (Sigma) using a thin-walled glass pipette (WPI) pulled to a ~50 μm tip. .. Coronal cortical slices (350–400 μm thickness) were cut using a vibrating microtome (Microm HM650V, Carl Zeiss Ltd) and slices were maintained in an interface chamber between humidified carbogen gas (95% O2 , 5% CO2 ) and ACSF (at 20–25°C).

Slice Preparation:

Article Title: A genetically-encoded chloride and pH sensor for dissociating ion dynamics in the nervous system
Article Snippet: Paragraph title: Slice preparation and DNA transfection ... Coronal cortical slices (350–400 μm thickness) were cut using a vibrating microtome (Microm HM650V, Carl Zeiss Ltd) and slices were maintained in an interface chamber between humidified carbogen gas (95% O2 , 5% CO2 ) and ACSF (at 20–25°C).

In Situ Hybridization:

Article Title: Cell-specific expression of tryptophan decarboxylase and 10-hydroxygeraniol oxidoreductase, key genes involved in camptothecin biosynthesis in Camptotheca acuminata Decne (Nyssaceae)
Article Snippet: Paragraph title: In situ hybridization ... The paraffin-embedded samples were sectioned (8-10 μm) using a microtome (Zeiss).

Plasmid Preparation:

Article Title: A genetically-encoded chloride and pH sensor for dissociating ion dynamics in the nervous system
Article Snippet: Each embryo was injected through the uterine wall with 0.5–1 ul ClopHensorN plasmid (2 ug/ul) in PBS with 0.03% fast green (Sigma) using a thin-walled glass pipette (WPI) pulled to a ~50 μm tip. .. Coronal cortical slices (350–400 μm thickness) were cut using a vibrating microtome (Microm HM650V, Carl Zeiss Ltd) and slices were maintained in an interface chamber between humidified carbogen gas (95% O2 , 5% CO2 ) and ACSF (at 20–25°C).

Imaging:

Article Title: A genetically-encoded chloride and pH sensor for dissociating ion dynamics in the nervous system
Article Snippet: Coronal cortical slices (350–400 μm thickness) were cut using a vibrating microtome (Microm HM650V, Carl Zeiss Ltd) and slices were maintained in an interface chamber between humidified carbogen gas (95% O2 , 5% CO2 ) and ACSF (at 20–25°C). .. After recovering for at least 1 h, the slices were mounted on coverslips (coated with 0.1% poly-L-lysine in ultrapure H2 O) before being transferred to the recording chamber for imaging.

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  • 93
    Carl Zeiss microtma slides
    Effects of doxorubicin (DOX) treatment(s) on cell viability, tumor size and caspase or Ki67 expression in glioblastoma and non-tumor (normal) spheroid tissues. Cleaved caspase 3 and Ki67 expression in tumour cells were measured by first segmentation in the GFP channel followed by thresholding in the caspase/ki67 channel facilitating the measurement of these end-points (both expressed as percentages of caspase/Ki67 positive cells relative to total cells) differentially in tumour cells and normal neuronal cells (see materials and methods section 4.8). Panel (A) shows effects of DOX treatments (0.025–0.5 uM) on cell viability (relative to control) as measured by the resazurin assay. Results are means ± SD, n = 3. Panel (B) shows effects of DOX treatment (0.025–0.3 uM) on the total number of non-tumor (normal neuronal) cells and the total number of glioblastoma cells in the gBS spheroid sections. Values are expressed as percentages of the total numbers of cells (normal + tumour) in the spheroid sections. Panel (C) shows effects of DOX treatment (0.025–0.3 uM) on the number of cleaved caspase 3 positive glioblastoma cells and non-tumor (normal neuronal) cells in representative dual immunofluorescence (GFP/cleaved caspase 3) stained gBS sections from the <t>microTMA:</t> a - control; b - 0.025 uM DOX; c - 0.05 uM DOX; d - 0.1 uM DOX; e - 0.3 uM DOX. Panel (D) shows the number of cleaved caspase 3 positive tumour (glioblastoma) cells and non-tumour cells in the spheroid sections expressed as a percentage of the total cells in the spheroid section. Values are expressed as percentages of the total numbers of glioblastoma or normal neuronal cells in the spheroid sections. Panel (E) shows effects of doxorubicin (DOX) treatment (0.025–0.3 uM) on the number of Ki67 positive glioblastoma cells and neuronal cells in representative dual immunofluorescence (GFP/Ki67) stained gBS sections from the microTMA: a - control; b - 0.025 uM DOX; c - 0.05 uM DOX; d - 0.1 uM DOX; e - 0.3 uM DOX. Panel (F) shows the number of Ki67 positive tumor cells and normal cells in the spheroid sections. Values are expressed as percentages of the total numbers of cells (normal + tumour) in the spheroid sections. Box plots show the median (black horizontal line) and the upper and lower quartiles (ends of the boxes); n = 32. Open circles show outliers > 1.5 times the interquartile range away from the upper and lower quartiles. ***Significantly different from control p
    Microtma Slides, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/microtma slides/product/Carl Zeiss
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    microtma slides - by Bioz Stars, 2020-04
    93/100 stars
      Buy from Supplier

    92
    Carl Zeiss 3view microtome
    (A) Conventional setup of the <t>3View</t> system. The software SmartSEM (Carl Zeiss Microscopy, Cambridge, United Kingdom), running on the EM Server PC, controls the scanning electron microscope (SEM). The software DigitalMicrograph (Gatan), running on a support PC, controls the 3View hardware (diamond knife and motorized stage). DigitalMicrograph can also indirectly control the SEM via SmartSEM . (B) SBEMimage interacts with SmartSEM through a proprietary remote API, provided by Carl Zeiss Microscopy, and with DigitalMicrograph through a custom-written communication script. SBEMimage can thus control all relevant low-level functions of the SBEM system and exert full control over the acquisition process.
    3view Microtome, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/3view microtome/product/Carl Zeiss
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    3view microtome - by Bioz Stars, 2020-04
    92/100 stars
      Buy from Supplier

    93
    Carl Zeiss microtome
    (A) Conventional setup of the <t>3View</t> system. The software SmartSEM (Carl Zeiss Microscopy, Cambridge, United Kingdom), running on the EM Server PC, controls the scanning electron microscope (SEM). The software DigitalMicrograph (Gatan), running on a support PC, controls the 3View hardware (diamond knife and motorized stage). DigitalMicrograph can also indirectly control the SEM via SmartSEM . (B) SBEMimage interacts with SmartSEM through a proprietary remote API, provided by Carl Zeiss Microscopy, and with DigitalMicrograph through a custom-written communication script. SBEMimage can thus control all relevant low-level functions of the SBEM system and exert full control over the acquisition process.
    Microtome, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/microtome/product/Carl Zeiss
    Average 93 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    microtome - by Bioz Stars, 2020-04
    93/100 stars
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    Effects of doxorubicin (DOX) treatment(s) on cell viability, tumor size and caspase or Ki67 expression in glioblastoma and non-tumor (normal) spheroid tissues. Cleaved caspase 3 and Ki67 expression in tumour cells were measured by first segmentation in the GFP channel followed by thresholding in the caspase/ki67 channel facilitating the measurement of these end-points (both expressed as percentages of caspase/Ki67 positive cells relative to total cells) differentially in tumour cells and normal neuronal cells (see materials and methods section 4.8). Panel (A) shows effects of DOX treatments (0.025–0.5 uM) on cell viability (relative to control) as measured by the resazurin assay. Results are means ± SD, n = 3. Panel (B) shows effects of DOX treatment (0.025–0.3 uM) on the total number of non-tumor (normal neuronal) cells and the total number of glioblastoma cells in the gBS spheroid sections. Values are expressed as percentages of the total numbers of cells (normal + tumour) in the spheroid sections. Panel (C) shows effects of DOX treatment (0.025–0.3 uM) on the number of cleaved caspase 3 positive glioblastoma cells and non-tumor (normal neuronal) cells in representative dual immunofluorescence (GFP/cleaved caspase 3) stained gBS sections from the microTMA: a - control; b - 0.025 uM DOX; c - 0.05 uM DOX; d - 0.1 uM DOX; e - 0.3 uM DOX. Panel (D) shows the number of cleaved caspase 3 positive tumour (glioblastoma) cells and non-tumour cells in the spheroid sections expressed as a percentage of the total cells in the spheroid section. Values are expressed as percentages of the total numbers of glioblastoma or normal neuronal cells in the spheroid sections. Panel (E) shows effects of doxorubicin (DOX) treatment (0.025–0.3 uM) on the number of Ki67 positive glioblastoma cells and neuronal cells in representative dual immunofluorescence (GFP/Ki67) stained gBS sections from the microTMA: a - control; b - 0.025 uM DOX; c - 0.05 uM DOX; d - 0.1 uM DOX; e - 0.3 uM DOX. Panel (F) shows the number of Ki67 positive tumor cells and normal cells in the spheroid sections. Values are expressed as percentages of the total numbers of cells (normal + tumour) in the spheroid sections. Box plots show the median (black horizontal line) and the upper and lower quartiles (ends of the boxes); n = 32. Open circles show outliers > 1.5 times the interquartile range away from the upper and lower quartiles. ***Significantly different from control p

    Journal: Scientific Reports

    Article Title: A Human iPSC-derived 3D platform using primary brain cancer cells to study drug development and personalized medicine

    doi: 10.1038/s41598-018-38130-0

    Figure Lengend Snippet: Effects of doxorubicin (DOX) treatment(s) on cell viability, tumor size and caspase or Ki67 expression in glioblastoma and non-tumor (normal) spheroid tissues. Cleaved caspase 3 and Ki67 expression in tumour cells were measured by first segmentation in the GFP channel followed by thresholding in the caspase/ki67 channel facilitating the measurement of these end-points (both expressed as percentages of caspase/Ki67 positive cells relative to total cells) differentially in tumour cells and normal neuronal cells (see materials and methods section 4.8). Panel (A) shows effects of DOX treatments (0.025–0.5 uM) on cell viability (relative to control) as measured by the resazurin assay. Results are means ± SD, n = 3. Panel (B) shows effects of DOX treatment (0.025–0.3 uM) on the total number of non-tumor (normal neuronal) cells and the total number of glioblastoma cells in the gBS spheroid sections. Values are expressed as percentages of the total numbers of cells (normal + tumour) in the spheroid sections. Panel (C) shows effects of DOX treatment (0.025–0.3 uM) on the number of cleaved caspase 3 positive glioblastoma cells and non-tumor (normal neuronal) cells in representative dual immunofluorescence (GFP/cleaved caspase 3) stained gBS sections from the microTMA: a - control; b - 0.025 uM DOX; c - 0.05 uM DOX; d - 0.1 uM DOX; e - 0.3 uM DOX. Panel (D) shows the number of cleaved caspase 3 positive tumour (glioblastoma) cells and non-tumour cells in the spheroid sections expressed as a percentage of the total cells in the spheroid section. Values are expressed as percentages of the total numbers of glioblastoma or normal neuronal cells in the spheroid sections. Panel (E) shows effects of doxorubicin (DOX) treatment (0.025–0.3 uM) on the number of Ki67 positive glioblastoma cells and neuronal cells in representative dual immunofluorescence (GFP/Ki67) stained gBS sections from the microTMA: a - control; b - 0.025 uM DOX; c - 0.05 uM DOX; d - 0.1 uM DOX; e - 0.3 uM DOX. Panel (F) shows the number of Ki67 positive tumor cells and normal cells in the spheroid sections. Values are expressed as percentages of the total numbers of cells (normal + tumour) in the spheroid sections. Box plots show the median (black horizontal line) and the upper and lower quartiles (ends of the boxes); n = 32. Open circles show outliers > 1.5 times the interquartile range away from the upper and lower quartiles. ***Significantly different from control p

    Article Snippet: Automated Scanning and Multispectral Image analysis of microTMA slides As the manual image acquisition process on the Zeiss LSM 710 confocal (above) was laborious we explored the possibility of automating the process using a PerkinElmer Vectra Polaris scanner (Vectra Polaris software version 1.0.6).

    Techniques: Expressing, Resazurin Assay, Immunofluorescence, Staining

    Construction and image analysis of a spheroid tissue microarray (microTMA). Fixed spheroids were embedded in a microTMA mold prior to paraffin embedding and sectioning on a microtome. TMA sections were then immunofluorescence stained and imaged using confocal scanning followed by dearraying of the spheroid images and image analysis (see material and methods section 4.8). ( A ) Schematic showing construction and processing of the microTMA for high-throughput histology and image analysis of 3D spheroid cultures. ( B ) Image analysis process to quantify tumor size and the number of cleaved caspase 3 or Ki67 positive tumor cells or normal neuronal cells. a dual immunofluorescence stained section showing GFP positive cells (red) and Ki67 positive cells (green), blue cells (DAPI) are normal (non-tumor cells); b - spectral unmixing and removal of autofluorescence; c, d - segmentation in the red channel to define tumor and normal cells/areas; e–h: segmentation in the green channel to define Ki67 or caspase positive cells, which are then counted in the tumor- and normal-cell regions.

    Journal: Scientific Reports

    Article Title: A Human iPSC-derived 3D platform using primary brain cancer cells to study drug development and personalized medicine

    doi: 10.1038/s41598-018-38130-0

    Figure Lengend Snippet: Construction and image analysis of a spheroid tissue microarray (microTMA). Fixed spheroids were embedded in a microTMA mold prior to paraffin embedding and sectioning on a microtome. TMA sections were then immunofluorescence stained and imaged using confocal scanning followed by dearraying of the spheroid images and image analysis (see material and methods section 4.8). ( A ) Schematic showing construction and processing of the microTMA for high-throughput histology and image analysis of 3D spheroid cultures. ( B ) Image analysis process to quantify tumor size and the number of cleaved caspase 3 or Ki67 positive tumor cells or normal neuronal cells. a dual immunofluorescence stained section showing GFP positive cells (red) and Ki67 positive cells (green), blue cells (DAPI) are normal (non-tumor cells); b - spectral unmixing and removal of autofluorescence; c, d - segmentation in the red channel to define tumor and normal cells/areas; e–h: segmentation in the green channel to define Ki67 or caspase positive cells, which are then counted in the tumor- and normal-cell regions.

    Article Snippet: Automated Scanning and Multispectral Image analysis of microTMA slides As the manual image acquisition process on the Zeiss LSM 710 confocal (above) was laborious we explored the possibility of automating the process using a PerkinElmer Vectra Polaris scanner (Vectra Polaris software version 1.0.6).

    Techniques: Microarray, Immunofluorescence, Staining, High Throughput Screening Assay

    (A) Conventional setup of the 3View system. The software SmartSEM (Carl Zeiss Microscopy, Cambridge, United Kingdom), running on the EM Server PC, controls the scanning electron microscope (SEM). The software DigitalMicrograph (Gatan), running on a support PC, controls the 3View hardware (diamond knife and motorized stage). DigitalMicrograph can also indirectly control the SEM via SmartSEM . (B) SBEMimage interacts with SmartSEM through a proprietary remote API, provided by Carl Zeiss Microscopy, and with DigitalMicrograph through a custom-written communication script. SBEMimage can thus control all relevant low-level functions of the SBEM system and exert full control over the acquisition process.

    Journal: Frontiers in Neural Circuits

    Article Title: SBEMimage: Versatile Acquisition Control Software for Serial Block-Face Electron Microscopy

    doi: 10.3389/fncir.2018.00054

    Figure Lengend Snippet: (A) Conventional setup of the 3View system. The software SmartSEM (Carl Zeiss Microscopy, Cambridge, United Kingdom), running on the EM Server PC, controls the scanning electron microscope (SEM). The software DigitalMicrograph (Gatan), running on a support PC, controls the 3View hardware (diamond knife and motorized stage). DigitalMicrograph can also indirectly control the SEM via SmartSEM . (B) SBEMimage interacts with SmartSEM through a proprietary remote API, provided by Carl Zeiss Microscopy, and with DigitalMicrograph through a custom-written communication script. SBEMimage can thus control all relevant low-level functions of the SBEM system and exert full control over the acquisition process.

    Article Snippet: SBEMimage is currently designed to operate a 3View microtome combined with a ZEISS Merlin microscope.

    Techniques: Software, Microscopy