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Becton Dickinson microtest
Microtest, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 88 stars, based on 1 article reviews
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Article Title: Trypanosoma cruzi: Synergistic cytotoxicity of multiple amphipathic anti-microbial peptides to T. cruzi and potential bacterial hosts
Article Snippet: One microliter of the peptide dilutions at 100× concentrations were added to the wells of sterile 96-well “Microtest” assay dishes (Becton Dickinson) in triplicate.

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    Becton Dickinson pdms micropost array membrane mpam
    Live-cell subcellular measurement of cell stiffness using a microengineered stretchable <t>micropost</t> array membrane <t>(mPAM)</t> and a cell stretching device (CSD). ( a ) Schematic of the CSD and its implementation for stretching the mPAM and thus single cells adhered
    Pdms Micropost Array Membrane Mpam, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pdms micropost array membrane mpam/product/Becton Dickinson
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pdms micropost array membrane mpam - by Bioz Stars, 2020-07
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    Becton Dickinson microtiter wells
    Binding of 125 I-labeled CS6 protein to serial dilutions of pure glycosphingolipids in <t>microtiter</t> wells. The assay was performed as described in the Materials and methods section. The results from one representative experiment out of three is shown.
    Microtiter Wells, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 92/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/microtiter wells/product/Becton Dickinson
    Average 92 stars, based on 27 article reviews
    Price from $9.99 to $1999.99
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    Live-cell subcellular measurement of cell stiffness using a microengineered stretchable micropost array membrane (mPAM) and a cell stretching device (CSD). ( a ) Schematic of the CSD and its implementation for stretching the mPAM and thus single cells adhered

    Journal: Integrative biology : quantitative biosciences from nano to macro

    Article Title: Live-cell subcellular measurement of cell stiffness using a microengineered stretchable micropost array membrane

    doi: 10.1039/c2ib20134h

    Figure Lengend Snippet: Live-cell subcellular measurement of cell stiffness using a microengineered stretchable micropost array membrane (mPAM) and a cell stretching device (CSD). ( a ) Schematic of the CSD and its implementation for stretching the mPAM and thus single cells adhered

    Article Snippet: The stretchable PDMS micropost array membrane (mPAM) was fabricated by spin-coating PDMS prepolymer (10:1 w / w ratio between the PDMS base monomer and curing agent) on the lids of Petri dishes (100 mm diameter; BD Falcon, Franklin Lakes, NJ) at 500 rpm for 1 min to obtain a 100 μm thick PDMS membrane, followed by baking the Petri dish lids at 60°C for 48 hr.

    Techniques:

    Subcellular measurement of cell stiffness for single fixed vascular smooth muscle cells (VSMCs). ( a ) Representative single fixed and stained VSMCs adhered on the PDMS micropost array before ( left ) and after ( right ) a 9% static equibiaxial mPAM stretch.

    Journal: Integrative biology : quantitative biosciences from nano to macro

    Article Title: Live-cell subcellular measurement of cell stiffness using a microengineered stretchable micropost array membrane

    doi: 10.1039/c2ib20134h

    Figure Lengend Snippet: Subcellular measurement of cell stiffness for single fixed vascular smooth muscle cells (VSMCs). ( a ) Representative single fixed and stained VSMCs adhered on the PDMS micropost array before ( left ) and after ( right ) a 9% static equibiaxial mPAM stretch.

    Article Snippet: The stretchable PDMS micropost array membrane (mPAM) was fabricated by spin-coating PDMS prepolymer (10:1 w / w ratio between the PDMS base monomer and curing agent) on the lids of Petri dishes (100 mm diameter; BD Falcon, Franklin Lakes, NJ) at 500 rpm for 1 min to obtain a 100 μm thick PDMS membrane, followed by baking the Petri dish lids at 60°C for 48 hr.

    Techniques: Staining

    Binding of 125 I-labeled CS6 protein to serial dilutions of pure glycosphingolipids in microtiter wells. The assay was performed as described in the Materials and methods section. The results from one representative experiment out of three is shown.

    Journal: PLoS ONE

    Article Title: Sulfatide Recognition by Colonization Factor Antigen CS6 from Enterotoxigenic Escherichia coli

    doi: 10.1371/journal.pone.0004487

    Figure Lengend Snippet: Binding of 125 I-labeled CS6 protein to serial dilutions of pure glycosphingolipids in microtiter wells. The assay was performed as described in the Materials and methods section. The results from one representative experiment out of three is shown.

    Article Snippet: In short, serial dilutions (each dilution in triplicate) of pure glycosphingolipids in methanol were applied in microtiter wells (Falcon 3911, Becton Dickinson Labware, Oxnard, CA).

    Techniques: Binding Assay, Labeling

    Effects of incubation of CS6 protein and recombinant CS6-expressing E. coli with dextran and dextran sulfate. Radiolabeled CS6 protein and recombinant CS6-expressing bacteria were incubated with dextran or dextran sulfate (0.01–1 mg/ml) for 1 h at room temperature. Thereafter the suspensions were utilized in the chromatogram binding assay or the microtiter well assay as described under “ Materials and Methods ”. Autoradiograms obtained by binding of 35 S-labeled E. coli TOP10-CS6 strain incubated with dextran (A), and with dextran sulfate (B). Autoradiography was for 12 h. The lanes were: Lane 1, sulfatide (SO 3 -3Galβ1Cer), 4 µg; Lane 2, sulfatide, 2 µg; Lane 3, sulfatide, 1 µg. Binding of 125 I-labeled CS6 protein, and 125 I-labeled CS6 protein incubated with dextran sulfate, to pure glycosphingolipids in microtiter wells (C).

    Journal: PLoS ONE

    Article Title: Sulfatide Recognition by Colonization Factor Antigen CS6 from Enterotoxigenic Escherichia coli

    doi: 10.1371/journal.pone.0004487

    Figure Lengend Snippet: Effects of incubation of CS6 protein and recombinant CS6-expressing E. coli with dextran and dextran sulfate. Radiolabeled CS6 protein and recombinant CS6-expressing bacteria were incubated with dextran or dextran sulfate (0.01–1 mg/ml) for 1 h at room temperature. Thereafter the suspensions were utilized in the chromatogram binding assay or the microtiter well assay as described under “ Materials and Methods ”. Autoradiograms obtained by binding of 35 S-labeled E. coli TOP10-CS6 strain incubated with dextran (A), and with dextran sulfate (B). Autoradiography was for 12 h. The lanes were: Lane 1, sulfatide (SO 3 -3Galβ1Cer), 4 µg; Lane 2, sulfatide, 2 µg; Lane 3, sulfatide, 1 µg. Binding of 125 I-labeled CS6 protein, and 125 I-labeled CS6 protein incubated with dextran sulfate, to pure glycosphingolipids in microtiter wells (C).

    Article Snippet: In short, serial dilutions (each dilution in triplicate) of pure glycosphingolipids in methanol were applied in microtiter wells (Falcon 3911, Becton Dickinson Labware, Oxnard, CA).

    Techniques: Incubation, Recombinant, Expressing, Binding Assay, Labeling, Autoradiography

    Binding of TN-R 160 and TN-R 180 to polar GLs, as determined by solid phase ligand-binding assay. The fraction of brain polar GLs ( br. GLs , 2 μg/ml in PBS) or Sulf , GalC , galactosyl diglyceride ( GDG ), psychosine ( psy. ), GM1 , and sphingosine ( sphi. , all at 1 μg/ml in PBS) were coated into wells of microtiter plates, and the binding of biotinylated TN-R was determined after 2 hr of incubation at 37°C ( A ). B , Immobilized Sulf (at 0.5 μg/ml in 100% ethanol) was incubated with increasing amounts of TN-R 160 or TN-R 180 (2–50 μg/ml). Bound protein was detected by a subsequent incubation with polyclonal antibodies to TN-R (2 hr at 37°C), and antibody binding was visualized by using HRP-conjugated goat anti-rabbit IgG and ABTS (2,2-azino-di-[3-ethylbenzthiazoline sulfonate(6)]; Boehringer Mannheim). Background binding to BSA was subtracted from the values obtained for the binding to different GLs. The maximal absorbance at 405 nm for the binding of each TN-R isoform to Sulf was set as 1.0. Values represent the mean ± SD of three ( A ) or two ( B ) experiments performed in triplicate.

    Journal: The Journal of Neuroscience

    Article Title: Tenascin-R Is an Intrinsic Autocrine Factor for Oligodendrocyte Differentiation and Promotes Cell Adhesion by a SulfatideMediated Mechanism

    doi: 10.1523/JNEUROSCI.17-12-04642.1997

    Figure Lengend Snippet: Binding of TN-R 160 and TN-R 180 to polar GLs, as determined by solid phase ligand-binding assay. The fraction of brain polar GLs ( br. GLs , 2 μg/ml in PBS) or Sulf , GalC , galactosyl diglyceride ( GDG ), psychosine ( psy. ), GM1 , and sphingosine ( sphi. , all at 1 μg/ml in PBS) were coated into wells of microtiter plates, and the binding of biotinylated TN-R was determined after 2 hr of incubation at 37°C ( A ). B , Immobilized Sulf (at 0.5 μg/ml in 100% ethanol) was incubated with increasing amounts of TN-R 160 or TN-R 180 (2–50 μg/ml). Bound protein was detected by a subsequent incubation with polyclonal antibodies to TN-R (2 hr at 37°C), and antibody binding was visualized by using HRP-conjugated goat anti-rabbit IgG and ABTS (2,2-azino-di-[3-ethylbenzthiazoline sulfonate(6)]; Boehringer Mannheim). Background binding to BSA was subtracted from the values obtained for the binding to different GLs. The maximal absorbance at 405 nm for the binding of each TN-R isoform to Sulf was set as 1.0. Values represent the mean ± SD of three ( A ) or two ( B ) experiments performed in triplicate.

    Article Snippet: Wells of microtiter plates (Falcon 3912; Becton Dickinson, Heidelberg, Germany) were coated with 100 μl/well of each GL (at a concentration of 0.5 or 1 μg/ml) for 1 hr at 37°C.

    Techniques: Binding Assay, Ligand Binding Assay, Incubation

    Quantification of attached biomass using a microtiter plate biofilm assay. The adherence of wild-type NZ131 and codY , covRS , and codY - covRS mutant strains to microtiter plates was quantified with crystal violet staining. Data presented are the averages and standard deviations of results from three independent experiments.

    Journal: Journal of Bacteriology

    Article Title: Counteractive Balancing of Transcriptome Expression Involving CodY and CovRS in Streptococcus pyogenes ▿ ▿ †

    doi: 10.1128/JB.00061-11

    Figure Lengend Snippet: Quantification of attached biomass using a microtiter plate biofilm assay. The adherence of wild-type NZ131 and codY , covRS , and codY - covRS mutant strains to microtiter plates was quantified with crystal violet staining. Data presented are the averages and standard deviations of results from three independent experiments.

    Article Snippet: In brief, microtiter wells (Falcon Microtest 96; Becton Dickinson) were inoculated from overnight C medium cultures diluted 1:60 in fresh C medium.

    Techniques: Biofilm Production Assay, Mutagenesis, Staining