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Becton Dickinson microtest plates
Microtest Plates, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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Enzyme-linked Immunosorbent Assay:

Article Title: Defensins Potentiate a Neutralizing Antibody Response to Enteric Viral Infection
Article Snippet: .. Anti-OVA antibodies were quantified by ELISA using microtest plates (BD Biosciences) incubated overnight with 50 μl/well of 125 μg/mL OVA in coating buffer (0.1 M Na2 CO3 , 0.1M NaHCO3 , pH 9.6) or coating buffer alone. .. Wells were blocked with ELISA block (5% nonfat dry milk, 0.05% Tween-20, 0.025% NaN3 , in PBS) for 1 h at 37°C, and heat inactivated (56°C for 30 min) serum diluted 20-fold into ELISA block was incubated overnight at 4°C in OVA-coated and control uncoated wells.

Incubation:

Article Title: Defensins Potentiate a Neutralizing Antibody Response to Enteric Viral Infection
Article Snippet: .. Anti-OVA antibodies were quantified by ELISA using microtest plates (BD Biosciences) incubated overnight with 50 μl/well of 125 μg/mL OVA in coating buffer (0.1 M Na2 CO3 , 0.1M NaHCO3 , pH 9.6) or coating buffer alone. .. Wells were blocked with ELISA block (5% nonfat dry milk, 0.05% Tween-20, 0.025% NaN3 , in PBS) for 1 h at 37°C, and heat inactivated (56°C for 30 min) serum diluted 20-fold into ELISA block was incubated overnight at 4°C in OVA-coated and control uncoated wells.

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  • 85
    Becton Dickinson polyvinyl chloride pvc plates
    Effect of azithromycin, clarithromycin, and moxifloxacin on the ability of M. avium to form <t>biofilm.</t> Bacteria were seeded onto a <t>PVC</t> plate and incubated with a subinhibitory concentration of azithromycin (8 μg/ml), clarithromycin (1 μg/ml), or moxifloxacin (1 μg/ml) for 14 days. Biofilm establishment was determined as described in the text. *, P
    Polyvinyl Chloride Pvc Plates, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Becton Dickinson microtiter plates
    Binding of TN-R 160 and TN-R 180 to polar GLs, as determined by solid phase ligand-binding assay. The fraction of brain polar GLs ( br. GLs , 2 μg/ml in PBS) or Sulf , GalC , galactosyl diglyceride ( GDG ), psychosine ( psy. ), GM1 , and sphingosine ( sphi. , all at 1 μg/ml in PBS) were coated into wells of <t>microtiter</t> plates, and the binding of biotinylated TN-R was determined after 2 hr of incubation at 37°C ( A ). B , Immobilized Sulf (at 0.5 μg/ml in 100% ethanol) was incubated with increasing amounts of TN-R 160 or TN-R 180 (2–50 μg/ml). Bound protein was detected by a subsequent incubation with polyclonal antibodies to TN-R (2 hr at 37°C), and antibody binding was visualized by using HRP-conjugated goat anti-rabbit IgG and ABTS (2,2-azino-di-[3-ethylbenzthiazoline sulfonate(6)]; Boehringer Mannheim). Background binding to BSA was subtracted from the values obtained for the binding to different GLs. The maximal absorbance at 405 nm for the binding of each TN-R isoform to Sulf was set as 1.0. Values represent the mean ± SD of three ( A ) or two ( B ) experiments performed in triplicate.
    Microtiter Plates, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 92/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Becton Dickinson static microtiter plate culture system
    P. aeruginosa PAO1 forms BLS within the ASM+. After 48 h of growth at 37°C under 20% EO 2 /static conditions, PAO1/pMRP9-1 developed BLS that were confined to the ASM+ and not attached to the surface of the <t>microtiter</t> plate. The composition of the ASM+ and the bacterial inoculation are described in Methods. The gelatinous mass containing the BLS was visualized in situ by CLSM. ( A ) CLSM micrograph of the PAO1/pMRP9-1 BLS; magnification, 10X; bar, 200.00 nm. ( B ) 3-D image analysis revealing the architecture of the BLS shown in (A); box, 800.00 pixels (px) W x 600 px H; bar, 100 px. ( C ) CLSM micrograph of the well bottom after the removal of the gelatinous mass showing no attached bacteria or biofilm (the scattered fluorescence observed is due to autofluorescing debris).
    Static Microtiter Plate Culture System, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 85/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Becton Dickinson 96 well flat bottomed microtiter plate
    Effect of in vitro maturation of mononuclear phagocytes on H 2 O 2 secretion stimulated by substrate-bound oxLDL or native LDL. Macrophages, 0–15 d in culture, were plated and incubated for 2 h at 37°C in <t>96-well</t> flat-bottomed <t>microtiter</t> plates precoated with 5–10 μg of oxLDL or native LDL, and H 2 O 2 secretion was measured, all as described for Fig. 3 A . Data are the average ± SEM of two to five experiments done in triplicate.
    96 Well Flat Bottomed Microtiter Plate, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Effect of azithromycin, clarithromycin, and moxifloxacin on the ability of M. avium to form biofilm. Bacteria were seeded onto a PVC plate and incubated with a subinhibitory concentration of azithromycin (8 μg/ml), clarithromycin (1 μg/ml), or moxifloxacin (1 μg/ml) for 14 days. Biofilm establishment was determined as described in the text. *, P

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: A Subinhibitory Concentration of Clarithromycin Inhibits Mycobacterium avium Biofilm Formation

    doi: 10.1128/AAC.48.12.4907-4910.2004

    Figure Lengend Snippet: Effect of azithromycin, clarithromycin, and moxifloxacin on the ability of M. avium to form biofilm. Bacteria were seeded onto a PVC plate and incubated with a subinhibitory concentration of azithromycin (8 μg/ml), clarithromycin (1 μg/ml), or moxifloxacin (1 μg/ml) for 14 days. Biofilm establishment was determined as described in the text. *, P

    Article Snippet: Briefly, well-developed biofilm colonies were removed from the polyvinyl chloride (PVC) plates (Falcon 3911, Microtest III flexible assay plate; Becton Dickinson, San Diego, Calif.) and resuspended in 5 ml of HBSS.

    Techniques: Incubation, Concentration Assay

    Scanning electron micrograph of M. avium 101 biofilm. Bacteria were seeded on a PVC plate (10 8 ).

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: A Subinhibitory Concentration of Clarithromycin Inhibits Mycobacterium avium Biofilm Formation

    doi: 10.1128/AAC.48.12.4907-4910.2004

    Figure Lengend Snippet: Scanning electron micrograph of M. avium 101 biofilm. Bacteria were seeded on a PVC plate (10 8 ).

    Article Snippet: Briefly, well-developed biofilm colonies were removed from the polyvinyl chloride (PVC) plates (Falcon 3911, Microtest III flexible assay plate; Becton Dickinson, San Diego, Calif.) and resuspended in 5 ml of HBSS.

    Techniques:

    Binding of TN-R 160 and TN-R 180 to polar GLs, as determined by solid phase ligand-binding assay. The fraction of brain polar GLs ( br. GLs , 2 μg/ml in PBS) or Sulf , GalC , galactosyl diglyceride ( GDG ), psychosine ( psy. ), GM1 , and sphingosine ( sphi. , all at 1 μg/ml in PBS) were coated into wells of microtiter plates, and the binding of biotinylated TN-R was determined after 2 hr of incubation at 37°C ( A ). B , Immobilized Sulf (at 0.5 μg/ml in 100% ethanol) was incubated with increasing amounts of TN-R 160 or TN-R 180 (2–50 μg/ml). Bound protein was detected by a subsequent incubation with polyclonal antibodies to TN-R (2 hr at 37°C), and antibody binding was visualized by using HRP-conjugated goat anti-rabbit IgG and ABTS (2,2-azino-di-[3-ethylbenzthiazoline sulfonate(6)]; Boehringer Mannheim). Background binding to BSA was subtracted from the values obtained for the binding to different GLs. The maximal absorbance at 405 nm for the binding of each TN-R isoform to Sulf was set as 1.0. Values represent the mean ± SD of three ( A ) or two ( B ) experiments performed in triplicate.

    Journal: The Journal of Neuroscience

    Article Title: Tenascin-R Is an Intrinsic Autocrine Factor for Oligodendrocyte Differentiation and Promotes Cell Adhesion by a SulfatideMediated Mechanism

    doi: 10.1523/JNEUROSCI.17-12-04642.1997

    Figure Lengend Snippet: Binding of TN-R 160 and TN-R 180 to polar GLs, as determined by solid phase ligand-binding assay. The fraction of brain polar GLs ( br. GLs , 2 μg/ml in PBS) or Sulf , GalC , galactosyl diglyceride ( GDG ), psychosine ( psy. ), GM1 , and sphingosine ( sphi. , all at 1 μg/ml in PBS) were coated into wells of microtiter plates, and the binding of biotinylated TN-R was determined after 2 hr of incubation at 37°C ( A ). B , Immobilized Sulf (at 0.5 μg/ml in 100% ethanol) was incubated with increasing amounts of TN-R 160 or TN-R 180 (2–50 μg/ml). Bound protein was detected by a subsequent incubation with polyclonal antibodies to TN-R (2 hr at 37°C), and antibody binding was visualized by using HRP-conjugated goat anti-rabbit IgG and ABTS (2,2-azino-di-[3-ethylbenzthiazoline sulfonate(6)]; Boehringer Mannheim). Background binding to BSA was subtracted from the values obtained for the binding to different GLs. The maximal absorbance at 405 nm for the binding of each TN-R isoform to Sulf was set as 1.0. Values represent the mean ± SD of three ( A ) or two ( B ) experiments performed in triplicate.

    Article Snippet: Wells of microtiter plates (Falcon 3912; Becton Dickinson, Heidelberg, Germany) were coated with 100 μl/well of each GL (at a concentration of 0.5 or 1 μg/ml) for 1 hr at 37°C.

    Techniques: Binding Assay, Ligand Binding Assay, Incubation

    P. aeruginosa PAO1 forms BLS within the ASM+. After 48 h of growth at 37°C under 20% EO 2 /static conditions, PAO1/pMRP9-1 developed BLS that were confined to the ASM+ and not attached to the surface of the microtiter plate. The composition of the ASM+ and the bacterial inoculation are described in Methods. The gelatinous mass containing the BLS was visualized in situ by CLSM. ( A ) CLSM micrograph of the PAO1/pMRP9-1 BLS; magnification, 10X; bar, 200.00 nm. ( B ) 3-D image analysis revealing the architecture of the BLS shown in (A); box, 800.00 pixels (px) W x 600 px H; bar, 100 px. ( C ) CLSM micrograph of the well bottom after the removal of the gelatinous mass showing no attached bacteria or biofilm (the scattered fluorescence observed is due to autofluorescing debris).

    Journal: BMC Microbiology

    Article Title: Characterization of biofilm-like structures formed by Pseudomonas aeruginosa in a synthetic mucus medium

    doi: 10.1186/1471-2180-12-181

    Figure Lengend Snippet: P. aeruginosa PAO1 forms BLS within the ASM+. After 48 h of growth at 37°C under 20% EO 2 /static conditions, PAO1/pMRP9-1 developed BLS that were confined to the ASM+ and not attached to the surface of the microtiter plate. The composition of the ASM+ and the bacterial inoculation are described in Methods. The gelatinous mass containing the BLS was visualized in situ by CLSM. ( A ) CLSM micrograph of the PAO1/pMRP9-1 BLS; magnification, 10X; bar, 200.00 nm. ( B ) 3-D image analysis revealing the architecture of the BLS shown in (A); box, 800.00 pixels (px) W x 600 px H; bar, 100 px. ( C ) CLSM micrograph of the well bottom after the removal of the gelatinous mass showing no attached bacteria or biofilm (the scattered fluorescence observed is due to autofluorescing debris).

    Article Snippet: Static microtiter plate culture system for development of the BLS Bacteria were grown in a static microtiter plate culture system using sterile 24-well polystyrene plates (Falcon; BD, Franklin Lakes, NJ) [ , ].

    Techniques: In Situ, Confocal Laser Scanning Microscopy, Fluorescence

    Effect of in vitro maturation of mononuclear phagocytes on H 2 O 2 secretion stimulated by substrate-bound oxLDL or native LDL. Macrophages, 0–15 d in culture, were plated and incubated for 2 h at 37°C in 96-well flat-bottomed microtiter plates precoated with 5–10 μg of oxLDL or native LDL, and H 2 O 2 secretion was measured, all as described for Fig. 3 A . Data are the average ± SEM of two to five experiments done in triplicate.

    Journal: The Journal of Experimental Medicine

    Article Title: Complementary Roles for Scavenger Receptor A and CD36 of Human Monocyte-derived Macrophages in Adhesion to Surfaces Coated with Oxidized Low-Density Lipoproteins and in Secretion of H2O2

    doi:

    Figure Lengend Snippet: Effect of in vitro maturation of mononuclear phagocytes on H 2 O 2 secretion stimulated by substrate-bound oxLDL or native LDL. Macrophages, 0–15 d in culture, were plated and incubated for 2 h at 37°C in 96-well flat-bottomed microtiter plates precoated with 5–10 μg of oxLDL or native LDL, and H 2 O 2 secretion was measured, all as described for Fig. 3 A . Data are the average ± SEM of two to five experiments done in triplicate.

    Article Snippet: Macrophages, cultured for the periods indicated in the figure legends, were washed, labeled with calcein/ AM for 40 min as described , washed three times with KRBG-A, and resuspended at 2.5 × 105 cells/ml in KRBG-A containing 5 mM EDTA, and 100 μl of this suspension was added to each well of a 96-well flat-bottomed microtiter plate (Falcon; Becton Dickinson Labware , Lincoln Park, NJ) which had been precoated with 5 μg oxLDL, acLDL, or native LDL per well, as described above.

    Techniques: In Vitro, Incubation

    Effect of catalase on detection of H 2 O 2 secreted by macrophages on oxLDL-coated surfaces. Macrophages (2 × 10 5 cells in 100 μl KRBG-A), cultured for 3–5 d, were plated on precoated surfaces and incubated in KRBG-A containing scopoletin/HRP and 1,800 U/ml catalase ( cat. ) for 2 h at 37°C. Each well of a 96-well microtiter plate was precoated with 10 μg of native LDL or oxLDL, as indicated. H 2 O 2 was measured as described for Fig. 3 A . Each point is the mean ± SEM nmoles H 2 O 2 from four experiments, each done in triplicate.

    Journal: The Journal of Experimental Medicine

    Article Title: Complementary Roles for Scavenger Receptor A and CD36 of Human Monocyte-derived Macrophages in Adhesion to Surfaces Coated with Oxidized Low-Density Lipoproteins and in Secretion of H2O2

    doi:

    Figure Lengend Snippet: Effect of catalase on detection of H 2 O 2 secreted by macrophages on oxLDL-coated surfaces. Macrophages (2 × 10 5 cells in 100 μl KRBG-A), cultured for 3–5 d, were plated on precoated surfaces and incubated in KRBG-A containing scopoletin/HRP and 1,800 U/ml catalase ( cat. ) for 2 h at 37°C. Each well of a 96-well microtiter plate was precoated with 10 μg of native LDL or oxLDL, as indicated. H 2 O 2 was measured as described for Fig. 3 A . Each point is the mean ± SEM nmoles H 2 O 2 from four experiments, each done in triplicate.

    Article Snippet: Macrophages, cultured for the periods indicated in the figure legends, were washed, labeled with calcein/ AM for 40 min as described , washed three times with KRBG-A, and resuspended at 2.5 × 105 cells/ml in KRBG-A containing 5 mM EDTA, and 100 μl of this suspension was added to each well of a 96-well flat-bottomed microtiter plate (Falcon; Becton Dickinson Labware , Lincoln Park, NJ) which had been precoated with 5 μg oxLDL, acLDL, or native LDL per well, as described above.

    Techniques: Cell Culture, Incubation

    Surface-bound, but not soluble, oxLDL stimulates H 2 O 2 secretion by macrophages. ( A ) Macrophages (2 × 10 5 cells in 100 μl KRBG-A), 4–7 d in culture, were plated and maintained in each well of 96-well microtiter plates for up to 3 h at 37°C in KRBG-A containing scopoletin/HRP. Each well was precoated with 10 μg of oxLDL or native LDL, or the medium contained 10 μg/100 μl oxLDL or native LDL. H 2 O 2 was measured as described in Materials and Methods. Data reported are the average ± SEM for H 2 O 2 secreted in three experiments done in triplicate. Statistical significance at the levels of * P

    Journal: The Journal of Experimental Medicine

    Article Title: Complementary Roles for Scavenger Receptor A and CD36 of Human Monocyte-derived Macrophages in Adhesion to Surfaces Coated with Oxidized Low-Density Lipoproteins and in Secretion of H2O2

    doi:

    Figure Lengend Snippet: Surface-bound, but not soluble, oxLDL stimulates H 2 O 2 secretion by macrophages. ( A ) Macrophages (2 × 10 5 cells in 100 μl KRBG-A), 4–7 d in culture, were plated and maintained in each well of 96-well microtiter plates for up to 3 h at 37°C in KRBG-A containing scopoletin/HRP. Each well was precoated with 10 μg of oxLDL or native LDL, or the medium contained 10 μg/100 μl oxLDL or native LDL. H 2 O 2 was measured as described in Materials and Methods. Data reported are the average ± SEM for H 2 O 2 secreted in three experiments done in triplicate. Statistical significance at the levels of * P

    Article Snippet: Macrophages, cultured for the periods indicated in the figure legends, were washed, labeled with calcein/ AM for 40 min as described , washed three times with KRBG-A, and resuspended at 2.5 × 105 cells/ml in KRBG-A containing 5 mM EDTA, and 100 μl of this suspension was added to each well of a 96-well flat-bottomed microtiter plate (Falcon; Becton Dickinson Labware , Lincoln Park, NJ) which had been precoated with 5 μg oxLDL, acLDL, or native LDL per well, as described above.

    Techniques:

    Effect of amount of surface-bound oxLDL on H 2 O 2 secretion by macrophages. Macrophages (2 × 10 5 in 100 μl KRBG-A), 4–7 d in culture, were plated and incubated for 2 h at 37°C in each well of 96-well microtiter plates precoated as indicated with 0.1–10 μg oxLDL. H 2 O 2 secretion was measured using scopoletin/HRP, as described for Fig. 3 A . Each point is the average ± SEM nmoles H 2 O 2 secreted in two to five experiments, each experiment done in triplicate.

    Journal: The Journal of Experimental Medicine

    Article Title: Complementary Roles for Scavenger Receptor A and CD36 of Human Monocyte-derived Macrophages in Adhesion to Surfaces Coated with Oxidized Low-Density Lipoproteins and in Secretion of H2O2

    doi:

    Figure Lengend Snippet: Effect of amount of surface-bound oxLDL on H 2 O 2 secretion by macrophages. Macrophages (2 × 10 5 in 100 μl KRBG-A), 4–7 d in culture, were plated and incubated for 2 h at 37°C in each well of 96-well microtiter plates precoated as indicated with 0.1–10 μg oxLDL. H 2 O 2 secretion was measured using scopoletin/HRP, as described for Fig. 3 A . Each point is the average ± SEM nmoles H 2 O 2 secreted in two to five experiments, each experiment done in triplicate.

    Article Snippet: Macrophages, cultured for the periods indicated in the figure legends, were washed, labeled with calcein/ AM for 40 min as described , washed three times with KRBG-A, and resuspended at 2.5 × 105 cells/ml in KRBG-A containing 5 mM EDTA, and 100 μl of this suspension was added to each well of a 96-well flat-bottomed microtiter plate (Falcon; Becton Dickinson Labware , Lincoln Park, NJ) which had been precoated with 5 μg oxLDL, acLDL, or native LDL per well, as described above.

    Techniques: Incubation

    Variation in H 2 O 2 secretion of macrophages from different donors plated on surfaces coated with oxLDL versus native LDL. Macrophages (2 × 10 5 in KRBG-A), 3–7 d in culture, were plated and incubated for 2 h at 37°C in 96-well microtiter plates precoated with 10 μg of oxLDL or native LDL. Each point is the average nmoles H 2 O 2 secreted in 19 experiments done in triplicate. The correlation line was calculated using the Origin 4.10 linear regression program.

    Journal: The Journal of Experimental Medicine

    Article Title: Complementary Roles for Scavenger Receptor A and CD36 of Human Monocyte-derived Macrophages in Adhesion to Surfaces Coated with Oxidized Low-Density Lipoproteins and in Secretion of H2O2

    doi:

    Figure Lengend Snippet: Variation in H 2 O 2 secretion of macrophages from different donors plated on surfaces coated with oxLDL versus native LDL. Macrophages (2 × 10 5 in KRBG-A), 3–7 d in culture, were plated and incubated for 2 h at 37°C in 96-well microtiter plates precoated with 10 μg of oxLDL or native LDL. Each point is the average nmoles H 2 O 2 secreted in 19 experiments done in triplicate. The correlation line was calculated using the Origin 4.10 linear regression program.

    Article Snippet: Macrophages, cultured for the periods indicated in the figure legends, were washed, labeled with calcein/ AM for 40 min as described , washed three times with KRBG-A, and resuspended at 2.5 × 105 cells/ml in KRBG-A containing 5 mM EDTA, and 100 μl of this suspension was added to each well of a 96-well flat-bottomed microtiter plate (Falcon; Becton Dickinson Labware , Lincoln Park, NJ) which had been precoated with 5 μg oxLDL, acLDL, or native LDL per well, as described above.

    Techniques: Incubation