Structured Review

Tecan Systems microplate reader
gga-miR-16-5p enhances IBDV-induced release of cytochrome c and activation of caspase-9 and caspase-3. (A and B) gga-miR-16-5p enhances IBDV-induced release of cytochrome c . DF-1 cells were transfected with the indicated miRNAs or miRNA controls. Twenty-four hours after transfection, cells were mock infected or infected with IBDV at an MOI of 0.1. At the indicated time points (24 and 48 h) after IBDV infection, cytosolic proteins were prepared and subjected to Western blot analysis for the measurement of cytochrome c in the cytosol of cells. The band densities of cytochrome c shown in panel A were quantitated by densitometry. The relative levels of cytochrome c were calculated as follows: band density of cytochrome c /that of β-actin. (C and D) gga-miR-16-5p enhances IBDV-induced activation of caspase-9 and caspase-3. DF-1 cells were treated as described for panel A, and at indicated time points after IBDV infection, the activities of caspase-9 and caspase-3 were measured at 405 nm with a <t>microplate</t> reader using the substrates LEHD-pNA (synthetic caspase-9 substrate) and DEVD-pNA (synthetic caspase-3 substrate). (E and F) Inhibition of caspase-3 suppresses IBDV replication. DF-1 cells were treated with 20 μM caspase-3 inhibitor (Z-DEVD-FMK) or dimethyl sulfoxide (DMSO) as a control for 2 h, followed by infection with IBDV at an MOI of 0.1. After 2 h, the cells were retreated with caspase-3 inhibitor or dimethyl sulfoxide as a control in fresh DMEM. At the indicated time points (12, 24, 48, and 72 h) post-IBDV infection, the viral titers in the cell cultures or supernatants were determined by TCID 50 analysis. The significance of the differences between caspase-3 inhibitor-treated cells and controls in terms of viral growth was determined by ANOVA ( P
Microplate Reader, supplied by Tecan Systems, used in various techniques. Bioz Stars score: 99/100, based on 1406 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/microplate reader/product/Tecan Systems
Average 99 stars, based on 1406 article reviews
Price from $9.99 to $1999.99
microplate reader - by Bioz Stars, 2020-09
99/100 stars

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1) Product Images from "Epigenetic Upregulation of Chicken MicroRNA-16-5p Expression in DF-1 Cells following Infection with Infectious Bursal Disease Virus (IBDV) Enhances IBDV-Induced Apoptosis and Viral Replication"

Article Title: Epigenetic Upregulation of Chicken MicroRNA-16-5p Expression in DF-1 Cells following Infection with Infectious Bursal Disease Virus (IBDV) Enhances IBDV-Induced Apoptosis and Viral Replication

Journal: Journal of Virology

doi: 10.1128/JVI.01724-19

gga-miR-16-5p enhances IBDV-induced release of cytochrome c and activation of caspase-9 and caspase-3. (A and B) gga-miR-16-5p enhances IBDV-induced release of cytochrome c . DF-1 cells were transfected with the indicated miRNAs or miRNA controls. Twenty-four hours after transfection, cells were mock infected or infected with IBDV at an MOI of 0.1. At the indicated time points (24 and 48 h) after IBDV infection, cytosolic proteins were prepared and subjected to Western blot analysis for the measurement of cytochrome c in the cytosol of cells. The band densities of cytochrome c shown in panel A were quantitated by densitometry. The relative levels of cytochrome c were calculated as follows: band density of cytochrome c /that of β-actin. (C and D) gga-miR-16-5p enhances IBDV-induced activation of caspase-9 and caspase-3. DF-1 cells were treated as described for panel A, and at indicated time points after IBDV infection, the activities of caspase-9 and caspase-3 were measured at 405 nm with a microplate reader using the substrates LEHD-pNA (synthetic caspase-9 substrate) and DEVD-pNA (synthetic caspase-3 substrate). (E and F) Inhibition of caspase-3 suppresses IBDV replication. DF-1 cells were treated with 20 μM caspase-3 inhibitor (Z-DEVD-FMK) or dimethyl sulfoxide (DMSO) as a control for 2 h, followed by infection with IBDV at an MOI of 0.1. After 2 h, the cells were retreated with caspase-3 inhibitor or dimethyl sulfoxide as a control in fresh DMEM. At the indicated time points (12, 24, 48, and 72 h) post-IBDV infection, the viral titers in the cell cultures or supernatants were determined by TCID 50 analysis. The significance of the differences between caspase-3 inhibitor-treated cells and controls in terms of viral growth was determined by ANOVA ( P
Figure Legend Snippet: gga-miR-16-5p enhances IBDV-induced release of cytochrome c and activation of caspase-9 and caspase-3. (A and B) gga-miR-16-5p enhances IBDV-induced release of cytochrome c . DF-1 cells were transfected with the indicated miRNAs or miRNA controls. Twenty-four hours after transfection, cells were mock infected or infected with IBDV at an MOI of 0.1. At the indicated time points (24 and 48 h) after IBDV infection, cytosolic proteins were prepared and subjected to Western blot analysis for the measurement of cytochrome c in the cytosol of cells. The band densities of cytochrome c shown in panel A were quantitated by densitometry. The relative levels of cytochrome c were calculated as follows: band density of cytochrome c /that of β-actin. (C and D) gga-miR-16-5p enhances IBDV-induced activation of caspase-9 and caspase-3. DF-1 cells were treated as described for panel A, and at indicated time points after IBDV infection, the activities of caspase-9 and caspase-3 were measured at 405 nm with a microplate reader using the substrates LEHD-pNA (synthetic caspase-9 substrate) and DEVD-pNA (synthetic caspase-3 substrate). (E and F) Inhibition of caspase-3 suppresses IBDV replication. DF-1 cells were treated with 20 μM caspase-3 inhibitor (Z-DEVD-FMK) or dimethyl sulfoxide (DMSO) as a control for 2 h, followed by infection with IBDV at an MOI of 0.1. After 2 h, the cells were retreated with caspase-3 inhibitor or dimethyl sulfoxide as a control in fresh DMEM. At the indicated time points (12, 24, 48, and 72 h) post-IBDV infection, the viral titers in the cell cultures or supernatants were determined by TCID 50 analysis. The significance of the differences between caspase-3 inhibitor-treated cells and controls in terms of viral growth was determined by ANOVA ( P

Techniques Used: Activation Assay, Transfection, Infection, Western Blot, Inhibition

2) Product Images from "Osteoking improves OP rat by enhancing HSP90-β expression"

Article Title: Osteoking improves OP rat by enhancing HSP90-β expression

Journal: International Journal of Molecular Medicine

doi: 10.3892/ijmm.2020.4529

Inhibition of HSP90-β decreases calcium deposition. The alizarin red staining intensities in osteoblasts was measured using a microplate reader. The values are expressed as the mean ± standard deviation (n=6). * P
Figure Legend Snippet: Inhibition of HSP90-β decreases calcium deposition. The alizarin red staining intensities in osteoblasts was measured using a microplate reader. The values are expressed as the mean ± standard deviation (n=6). * P

Techniques Used: Inhibition, Staining, Standard Deviation

3) Product Images from "Effects of a Protein Kinase Inhibitor on Sperm Motility in the Japanese Quail"

Article Title: Effects of a Protein Kinase Inhibitor on Sperm Motility in the Japanese Quail

Journal: The Journal of Poultry Science

doi: 10.2141/jpsa.0160079

Measurement of various sperm parameters in the presence of BisII. Intracellular pH (pH i ) (A), intracellular Ca 2+ concentration (B) and mitochondrial activity (C) were measured by spectrofluorometry. cAMP (D), ATP (E) and ATPase activity (F) in the sperm lysates were assayed using a microplate reader. Values are means±SD of 3 independent experiments. No significant difference was found between CONT (vehicle alone) and BisII (1 µ M) in all parameters tested. In panel B, calcium ionophore, A23187 (1 µ M) was added as a positive control. In panel C, uncoupler CCCP (10 µ M) was included as a negative control.
Figure Legend Snippet: Measurement of various sperm parameters in the presence of BisII. Intracellular pH (pH i ) (A), intracellular Ca 2+ concentration (B) and mitochondrial activity (C) were measured by spectrofluorometry. cAMP (D), ATP (E) and ATPase activity (F) in the sperm lysates were assayed using a microplate reader. Values are means±SD of 3 independent experiments. No significant difference was found between CONT (vehicle alone) and BisII (1 µ M) in all parameters tested. In panel B, calcium ionophore, A23187 (1 µ M) was added as a positive control. In panel C, uncoupler CCCP (10 µ M) was included as a negative control.

Techniques Used: Concentration Assay, Activity Assay, Positive Control, Negative Control

4) Product Images from "Downregulation of testosterone production through luteinizing hormone receptor regulation in male rats exposed to 17α-ethynylestradiol"

Article Title: Downregulation of testosterone production through luteinizing hormone receptor regulation in male rats exposed to 17α-ethynylestradiol

Journal: Scientific Reports

doi: 10.1038/s41598-020-58125-0

EE2 treatment reduces testosterone release in normal rat primary Leydig cells. EE2 inhibits testosterone release in vitro . ( A ) RIA for analyzing the concentration of testosterone in normal rat primary Leydig cells. Cells (1 × 10 5 cells per tube) were treated with EE2 (0.1–1000 nM) in the absence or presence of hCG (0.05 IU/ml) for 1 h. At the end of the incubation, culture media were collected for testosterone RIA. ( B ) Cell survival assay. Rat primary Leydig cells (1 × 10 5 cells per well) were seeded in a 96-well microplate and rested for 12 h. Subsequently, cells were treated with EE2 at different concentrations (0.1–10,000 nM) for 1 h. Then, the survival rate was measured by the MTT assay. Data represent means ± SEM (n = 5). * P
Figure Legend Snippet: EE2 treatment reduces testosterone release in normal rat primary Leydig cells. EE2 inhibits testosterone release in vitro . ( A ) RIA for analyzing the concentration of testosterone in normal rat primary Leydig cells. Cells (1 × 10 5 cells per tube) were treated with EE2 (0.1–1000 nM) in the absence or presence of hCG (0.05 IU/ml) for 1 h. At the end of the incubation, culture media were collected for testosterone RIA. ( B ) Cell survival assay. Rat primary Leydig cells (1 × 10 5 cells per well) were seeded in a 96-well microplate and rested for 12 h. Subsequently, cells were treated with EE2 at different concentrations (0.1–10,000 nM) for 1 h. Then, the survival rate was measured by the MTT assay. Data represent means ± SEM (n = 5). * P

Techniques Used: In Vitro, Concentration Assay, Incubation, Clonogenic Cell Survival Assay, MTT Assay

5) Product Images from "Effects of a Protein Kinase Inhibitor on Sperm Motility in the Japanese Quail"

Article Title: Effects of a Protein Kinase Inhibitor on Sperm Motility in the Japanese Quail

Journal: The Journal of Poultry Science

doi: 10.2141/jpsa.0160079

Measurement of various sperm parameters in the presence of BisII. Intracellular pH (pH i ) (A), intracellular Ca 2+ concentration (B) and mitochondrial activity (C) were measured by spectrofluorometry. cAMP (D), ATP (E) and ATPase activity (F) in the sperm lysates were assayed using a microplate reader. Values are means±SD of 3 independent experiments. No significant difference was found between CONT (vehicle alone) and BisII (1 µ M) in all parameters tested. In panel B, calcium ionophore, A23187 (1 µ M) was added as a positive control. In panel C, uncoupler CCCP (10 µ M) was included as a negative control.
Figure Legend Snippet: Measurement of various sperm parameters in the presence of BisII. Intracellular pH (pH i ) (A), intracellular Ca 2+ concentration (B) and mitochondrial activity (C) were measured by spectrofluorometry. cAMP (D), ATP (E) and ATPase activity (F) in the sperm lysates were assayed using a microplate reader. Values are means±SD of 3 independent experiments. No significant difference was found between CONT (vehicle alone) and BisII (1 µ M) in all parameters tested. In panel B, calcium ionophore, A23187 (1 µ M) was added as a positive control. In panel C, uncoupler CCCP (10 µ M) was included as a negative control.

Techniques Used: Concentration Assay, Activity Assay, Positive Control, Negative Control

Related Articles

Enzyme-linked Immunosorbent Assay:

Article Title: Downregulation of testosterone production through luteinizing hormone receptor regulation in male rats exposed to 17α-ethynylestradiol
Article Snippet: .. The optical density was measured by a microplate reader (TECAN Sunrise ELISA Reader, Männedorf, Switzerland) at 570 nm and 630 nm as the reference wavelength. .. Radioimmunoassay (RIA) of testosterone The concentrations of testosterone in the culture medium and plasma were determined by RIA as previously described .

Incubation:

Article Title: Different Types of Coagulase Are Associated With 28-Day Mortality in Patients With Staphylococcus aureus Bloodstream Infections
Article Snippet: .. For improved quantification, 150 μl 33% acetic acid (AnalaR Normapur, Prolabo®, VWR International®, USA) was placed in each well, and plates were incubated at 37°C and 50% humidity for 1 h. Plates were measured using a microplate reader (Sunrise, Tecan, Switzerland) at 595 nm with a reference measurement at 405 nm. ..

Article Title: Myoviridae phage PDX kills enteroaggregative Escherichia coli without human microbiome dysbiosis
Article Snippet: .. The plates were incubated at 37 °C with orbital shaking for 12 h and OD at 600 nm was measured using a Microtiter Plate Reader (Sunrise, Tecan Group Ltd, Austria) at 30 min intervals. ..

Spectrophotometry:

Article Title: Possible Factors Influencing the Seroprevalence of Dengue among Residents of the Forest Fringe Areas of Peninsular Malaysia
Article Snippet: .. The absorbance was read at 450/620 nm using a Tecan Sunrise spectrophotometer (Mannedorf, Switzerland). ..

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