micromonospora species  (DSMZ)

Journal: Marine Drugs

Article Title: A New Micromonospora Strain with Antibiotic Activity Isolated from the Microbiome of a Mid-Atlantic Deep-Sea Sponge

doi: 10.3390/md19020105

Figure Lengend Snippet: HPLC-ELSD chromatogram of the crude extract of Micromonospora sp. 28ISP2-46 T .

Article Snippet: The genomes of the type Ia Micromonospora species were annotated using antiSMASH 5.0 [ ] and manually inspected for BGCs with significant similarity to each BGC of strain 28ISP2-46 T . For the group Ia Micromonospora species, GenBank assembly accession numbers are as follows: Micromonospora aurantiaca ATCC 27,029 (GCA_000145235.1), Micromonospora auratinigra DSM 44,815 (GCA_900089595.1), Micromonospora chaiyaphumensis DSM 45,246 (GCA_900091435.1), Micromonospora chalcea DSM 43,026 (GCA_002926165.1), Micromonospora chersina DSM 44,151 (GCA_900091475.1), Micromonospora humi DSM 45,647 (GCA_900090105.1), Micromonospora marina DSM 45,555 (GCA_900091565.1), Micromonospora sediminicola DSM 45,794 (GCA_900089585.1) and Micromonospora tulbaghiae CNY-010 (GCA_003612775.1).

Techniques:

Journal: Marine Drugs

Article Title: A New Micromonospora Strain with Antibiotic Activity Isolated from the Microbiome of a Mid-Atlantic Deep-Sea Sponge

doi: 10.3390/md19020105

Figure Lengend Snippet: Phylogenetic tree for strain 28ISP2-46 T and the other group IA Micromonospora species generated using the TYGS and drawn with iTOL. The tree was constructed using FastME 2.1.6.1 from GBDP distances calculated from genome sequences. The branch lengths are scaled in terms of GBDP distance formula d5. The numbers above branches are GBDP pseudo-bootstrap support values from 100 replications, with an average branch support of 98.4% and a delta statistic of 0.135. The tree was rooted using Micromonospora pallida as the outgroup.

Article Snippet: The genomes of the type Ia Micromonospora species were annotated using antiSMASH 5.0 [ ] and manually inspected for BGCs with significant similarity to each BGC of strain 28ISP2-46 T . For the group Ia Micromonospora species, GenBank assembly accession numbers are as follows: Micromonospora aurantiaca ATCC 27,029 (GCA_000145235.1), Micromonospora auratinigra DSM 44,815 (GCA_900089595.1), Micromonospora chaiyaphumensis DSM 45,246 (GCA_900091435.1), Micromonospora chalcea DSM 43,026 (GCA_002926165.1), Micromonospora chersina DSM 44,151 (GCA_900091475.1), Micromonospora humi DSM 45,647 (GCA_900090105.1), Micromonospora marina DSM 45,555 (GCA_900091565.1), Micromonospora sediminicola DSM 45,794 (GCA_900089585.1) and Micromonospora tulbaghiae CNY-010 (GCA_003612775.1).

Techniques: Generated, Construct

Journal: Marine Drugs

Article Title: A New Micromonospora Strain with Antibiotic Activity Isolated from the Microbiome of a Mid-Atlantic Deep-Sea Sponge

doi: 10.3390/md19020105

Figure Lengend Snippet: Biosynthetic gene clusters identified in the genome of strain 28ISP2-46 T using AntiSMASH.

Article Snippet: The genomes of the type Ia Micromonospora species were annotated using antiSMASH 5.0 [ ] and manually inspected for BGCs with significant similarity to each BGC of strain 28ISP2-46 T . For the group Ia Micromonospora species, GenBank assembly accession numbers are as follows: Micromonospora aurantiaca ATCC 27,029 (GCA_000145235.1), Micromonospora auratinigra DSM 44,815 (GCA_900089595.1), Micromonospora chaiyaphumensis DSM 45,246 (GCA_900091435.1), Micromonospora chalcea DSM 43,026 (GCA_002926165.1), Micromonospora chersina DSM 44,151 (GCA_900091475.1), Micromonospora humi DSM 45,647 (GCA_900090105.1), Micromonospora marina DSM 45,555 (GCA_900091565.1), Micromonospora sediminicola DSM 45,794 (GCA_900089585.1) and Micromonospora tulbaghiae CNY-010 (GCA_003612775.1).

Techniques:

Journal: Marine Drugs

Article Title: A New Micromonospora Strain with Antibiotic Activity Isolated from the Microbiome of a Mid-Atlantic Deep-Sea Sponge

doi: 10.3390/md19020105

Figure Lengend Snippet: ( a ) ClusterBLAST hits for the kosinostatin cluster. Homologues of kst genes are coloured as follows: kstA genes red, kstB genes yellow, kstC genes green, kstD genes blue, kstRg genes black, kstRs genes white, and genes uninvolved in kosinostatin biosynthesis grey. A black line at the end of a cluster indicates that it is close to the end of a contig. Gene sizes not to scale. The NCBI protein IDs for first and last coloured genes of each cluster are as follows: Micromonospora sp. TP-A0468 AFJ52719.1 and AFJ52701.1; Micromonospora sp. 28ISP2-46 T QLQ36639.1 and QLQ36600.1; N. valliformis WP_017579213.1 and WP_017579258.1; N. alkaliphila WP_017604198.1 and WP_017604153.1; Streptomyces sp. SM8 PKA38649.1 and PKA38729.1; Streptomyces sp. ScaeMP-6W SCE31237.1 and SCE31955.1; A. bangkokensis OLR89622.1 and OLR89598.1; M. haikouensis SCF11188.1 and SCF11278.1. ( b ) The same gene clusters rearranged for ease of comparison. Coloured as 6a.

Article Snippet: The genomes of the type Ia Micromonospora species were annotated using antiSMASH 5.0 [ ] and manually inspected for BGCs with significant similarity to each BGC of strain 28ISP2-46 T . For the group Ia Micromonospora species, GenBank assembly accession numbers are as follows: Micromonospora aurantiaca ATCC 27,029 (GCA_000145235.1), Micromonospora auratinigra DSM 44,815 (GCA_900089595.1), Micromonospora chaiyaphumensis DSM 45,246 (GCA_900091435.1), Micromonospora chalcea DSM 43,026 (GCA_002926165.1), Micromonospora chersina DSM 44,151 (GCA_900091475.1), Micromonospora humi DSM 45,647 (GCA_900090105.1), Micromonospora marina DSM 45,555 (GCA_900091565.1), Micromonospora sediminicola DSM 45,794 (GCA_900089585.1) and Micromonospora tulbaghiae CNY-010 (GCA_003612775.1).

Techniques: Comparison

Journal: Marine Drugs

Article Title: A New Micromonospora Strain with Antibiotic Activity Isolated from the Microbiome of a Mid-Atlantic Deep-Sea Sponge

doi: 10.3390/md19020105

Figure Lengend Snippet: HPLC-ELSD chromatogram of the crude extract of Micromonospora sp. 28ISP2-46 T .

Article Snippet: The genomes of the type Ia Micromonospora species were annotated using antiSMASH 5.0 [ ] and manually inspected for BGCs with significant similarity to each BGC of strain 28ISP2-46 T . For the group Ia Micromonospora species, GenBank assembly accession numbers are as follows: Micromonospora aurantiaca ATCC 27,029 (GCA_000145235.1), Micromonospora auratinigra DSM 44,815 (GCA_900089595.1), Micromonospora chaiyaphumensis DSM 45,246 (GCA_900091435.1), Micromonospora chalcea DSM 43,026 (GCA_002926165.1), Micromonospora chersina DSM 44,151 (GCA_900091475.1), Micromonospora humi DSM 45,647 (GCA_900090105.1), Micromonospora marina DSM 45,555 (GCA_900091565.1), Micromonospora sediminicola DSM 45,794 (GCA_900089585.1) and Micromonospora tulbaghiae CNY-010 (GCA_003612775.1).

Techniques:

Journal: Marine Drugs

Article Title: A New Micromonospora Strain with Antibiotic Activity Isolated from the Microbiome of a Mid-Atlantic Deep-Sea Sponge

doi: 10.3390/md19020105

Figure Lengend Snippet: Phylogenetic tree for strain 28ISP2-46 T and the other group IA Micromonospora species generated using the TYGS and drawn with iTOL. The tree was constructed using FastME 2.1.6.1 from GBDP distances calculated from genome sequences. The branch lengths are scaled in terms of GBDP distance formula d5. The numbers above branches are GBDP pseudo-bootstrap support values from 100 replications, with an average branch support of 98.4% and a delta statistic of 0.135. The tree was rooted using Micromonospora pallida as the outgroup.

Article Snippet: The genomes of the type Ia Micromonospora species were annotated using antiSMASH 5.0 [ ] and manually inspected for BGCs with significant similarity to each BGC of strain 28ISP2-46 T . For the group Ia Micromonospora species, GenBank assembly accession numbers are as follows: Micromonospora aurantiaca ATCC 27,029 (GCA_000145235.1), Micromonospora auratinigra DSM 44,815 (GCA_900089595.1), Micromonospora chaiyaphumensis DSM 45,246 (GCA_900091435.1), Micromonospora chalcea DSM 43,026 (GCA_002926165.1), Micromonospora chersina DSM 44,151 (GCA_900091475.1), Micromonospora humi DSM 45,647 (GCA_900090105.1), Micromonospora marina DSM 45,555 (GCA_900091565.1), Micromonospora sediminicola DSM 45,794 (GCA_900089585.1) and Micromonospora tulbaghiae CNY-010 (GCA_003612775.1).

Techniques: Generated, Construct

Journal: Marine Drugs

Article Title: A New Micromonospora Strain with Antibiotic Activity Isolated from the Microbiome of a Mid-Atlantic Deep-Sea Sponge

doi: 10.3390/md19020105

Figure Lengend Snippet: Biosynthetic gene clusters identified in the genome of strain 28ISP2-46 T using AntiSMASH.

Article Snippet: The genomes of the type Ia Micromonospora species were annotated using antiSMASH 5.0 [ ] and manually inspected for BGCs with significant similarity to each BGC of strain 28ISP2-46 T . For the group Ia Micromonospora species, GenBank assembly accession numbers are as follows: Micromonospora aurantiaca ATCC 27,029 (GCA_000145235.1), Micromonospora auratinigra DSM 44,815 (GCA_900089595.1), Micromonospora chaiyaphumensis DSM 45,246 (GCA_900091435.1), Micromonospora chalcea DSM 43,026 (GCA_002926165.1), Micromonospora chersina DSM 44,151 (GCA_900091475.1), Micromonospora humi DSM 45,647 (GCA_900090105.1), Micromonospora marina DSM 45,555 (GCA_900091565.1), Micromonospora sediminicola DSM 45,794 (GCA_900089585.1) and Micromonospora tulbaghiae CNY-010 (GCA_003612775.1).

Techniques:

Journal: Marine Drugs

Article Title: A New Micromonospora Strain with Antibiotic Activity Isolated from the Microbiome of a Mid-Atlantic Deep-Sea Sponge

doi: 10.3390/md19020105

Figure Lengend Snippet: ( a ) ClusterBLAST hits for the kosinostatin cluster. Homologues of kst genes are coloured as follows: kstA genes red, kstB genes yellow, kstC genes green, kstD genes blue, kstRg genes black, kstRs genes white, and genes uninvolved in kosinostatin biosynthesis grey. A black line at the end of a cluster indicates that it is close to the end of a contig. Gene sizes not to scale. The NCBI protein IDs for first and last coloured genes of each cluster are as follows: Micromonospora sp. TP-A0468 AFJ52719.1 and AFJ52701.1; Micromonospora sp. 28ISP2-46 T QLQ36639.1 and QLQ36600.1; N. valliformis WP_017579213.1 and WP_017579258.1; N. alkaliphila WP_017604198.1 and WP_017604153.1; Streptomyces sp. SM8 PKA38649.1 and PKA38729.1; Streptomyces sp. ScaeMP-6W SCE31237.1 and SCE31955.1; A. bangkokensis OLR89622.1 and OLR89598.1; M. haikouensis SCF11188.1 and SCF11278.1. ( b ) The same gene clusters rearranged for ease of comparison. Coloured as 6a.

Article Snippet: The genomes of the type Ia Micromonospora species were annotated using antiSMASH 5.0 [ ] and manually inspected for BGCs with significant similarity to each BGC of strain 28ISP2-46 T . For the group Ia Micromonospora species, GenBank assembly accession numbers are as follows: Micromonospora aurantiaca ATCC 27,029 (GCA_000145235.1), Micromonospora auratinigra DSM 44,815 (GCA_900089595.1), Micromonospora chaiyaphumensis DSM 45,246 (GCA_900091435.1), Micromonospora chalcea DSM 43,026 (GCA_002926165.1), Micromonospora chersina DSM 44,151 (GCA_900091475.1), Micromonospora humi DSM 45,647 (GCA_900090105.1), Micromonospora marina DSM 45,555 (GCA_900091565.1), Micromonospora sediminicola DSM 45,794 (GCA_900089585.1) and Micromonospora tulbaghiae CNY-010 (GCA_003612775.1).

Techniques: Comparison

Journal: Frontiers in Microbiology

Article Title: Exploring the Potential of Antibiotic Production From Rare Actinobacteria by Whole-Genome Sequencing and Guided MS/MS Analysis

doi: 10.3389/fmicb.2020.01540

Figure Lengend Snippet: The endophytic actinobacterial community identified based on the partial 16S rRNA sequences.

Article Snippet: The isolate sp.01 showing 99.28% sequence similarity with a reference rare actinobacterial species Micromonospora aurantiaca ATCC 27029 T was selected for whole genome sequencing.

Techniques:

Journal: Frontiers in Microbiology

Article Title: Exploring the Potential of Antibiotic Production From Rare Actinobacteria by Whole-Genome Sequencing and Guided MS/MS Analysis

doi: 10.3389/fmicb.2020.01540

Figure Lengend Snippet: Phylogenetic tree of the 14 actinobacterial strains isolated from mangrove leaves. The tree was constructed with the neighbor-joining method having the number of bootstrap replications set to 1000.

Article Snippet: The isolate sp.01 showing 99.28% sequence similarity with a reference rare actinobacterial species Micromonospora aurantiaca ATCC 27029 T was selected for whole genome sequencing.

Techniques: Isolation, Construct