micrococcal nuclease mnase  (Millipore)


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    Structured Review

    Millipore micrococcal nuclease mnase
    Consensus GGUG-containing RNA oligonucleotide promotes the inhibitory effect of TLS on CBP/p300 HAT activities a , Co-immunoprecipitation (IP) of p300 and TLS from <t>RNase</t> A-treated HeLa cells. b, P300 HAT activity was measured using micrococcal nuclease <t>(MNase)</t> or DNase I pre-treated GST and GST-TLS in the presence of GGUG- or CCUC-oligonucleotide. * p
    Micrococcal Nuclease Mnase, supplied by Millipore, used in various techniques. Bioz Stars score: 98/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/micrococcal nuclease mnase/product/Millipore
    Average 98 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    micrococcal nuclease mnase - by Bioz Stars, 2020-05
    98/100 stars

    Images

    1) Product Images from "Induced ncRNAs Allosterically Modify RNA Binding Proteins in cis to Inhibit Transcription"

    Article Title: Induced ncRNAs Allosterically Modify RNA Binding Proteins in cis to Inhibit Transcription

    Journal: Nature

    doi: 10.1038/nature06992

    Consensus GGUG-containing RNA oligonucleotide promotes the inhibitory effect of TLS on CBP/p300 HAT activities a , Co-immunoprecipitation (IP) of p300 and TLS from RNase A-treated HeLa cells. b, P300 HAT activity was measured using micrococcal nuclease (MNase) or DNase I pre-treated GST and GST-TLS in the presence of GGUG- or CCUC-oligonucleotide. * p
    Figure Legend Snippet: Consensus GGUG-containing RNA oligonucleotide promotes the inhibitory effect of TLS on CBP/p300 HAT activities a , Co-immunoprecipitation (IP) of p300 and TLS from RNase A-treated HeLa cells. b, P300 HAT activity was measured using micrococcal nuclease (MNase) or DNase I pre-treated GST and GST-TLS in the presence of GGUG- or CCUC-oligonucleotide. * p

    Techniques Used: HAT Assay, Immunoprecipitation, Activity Assay

    2) Product Images from "Histone ADP-Ribosylation Facilitates Gene Transcription by Directly Remodeling Nucleosomes"

    Article Title: Histone ADP-Ribosylation Facilitates Gene Transcription by Directly Remodeling Nucleosomes

    Journal: Molecular and Cellular Biology

    doi: 10.1128/MCB.06667-11

    MNase-accessible chromatin contains ADPr-histones. (A) Control and LPS-stimulated macrophages were subjected to the PARP-1 enzymatic assay and were then immediately fixed with formaldehyde. (Top and center) Chromatin was isolated and independently digested
    Figure Legend Snippet: MNase-accessible chromatin contains ADPr-histones. (A) Control and LPS-stimulated macrophages were subjected to the PARP-1 enzymatic assay and were then immediately fixed with formaldehyde. (Top and center) Chromatin was isolated and independently digested

    Techniques Used: Enzymatic Assay, Isolation

    3) Product Images from "Genetic Inactivation of ATRX Leads to a Decrease in the Amount of Telomeric Cohesin and Level of Telomere Transcription in Human Glioma Cells"

    Article Title: Genetic Inactivation of ATRX Leads to a Decrease in the Amount of Telomeric Cohesin and Level of Telomere Transcription in Human Glioma Cells

    Journal: Molecular and Cellular Biology

    doi: 10.1128/MCB.01317-14

    shRNA-mediated inactivation of ATRX does not alter subtelomeric chromatin accessibility to MNase. (A) Chromatin isolated from 8-MG-BA glioma cells in which ATRX had been inactivated (shATRX) or not (shscrambled [shSCR]) was digested with MNase for the indicated times. (Left gel) Ethidium bromide (EtBr) staining of bulk chromatin. (Right gel) Southern blot with subtelomeric probe. (Far right) Quantification of the data. The signals obtained for mononucleosomes were normalized to the total signals measured for each time point (EtBr or Southern blot). (B) Chromatin samples from shATRX or shSCR 8-MG-BA cells were digested for 5 min with the indicated amounts of MNase (milliunits per microgram of DNA). (Far right) Quantification of the data.
    Figure Legend Snippet: shRNA-mediated inactivation of ATRX does not alter subtelomeric chromatin accessibility to MNase. (A) Chromatin isolated from 8-MG-BA glioma cells in which ATRX had been inactivated (shATRX) or not (shscrambled [shSCR]) was digested with MNase for the indicated times. (Left gel) Ethidium bromide (EtBr) staining of bulk chromatin. (Right gel) Southern blot with subtelomeric probe. (Far right) Quantification of the data. The signals obtained for mononucleosomes were normalized to the total signals measured for each time point (EtBr or Southern blot). (B) Chromatin samples from shATRX or shSCR 8-MG-BA cells were digested for 5 min with the indicated amounts of MNase (milliunits per microgram of DNA). (Far right) Quantification of the data.

    Techniques Used: shRNA, Isolation, Staining, Southern Blot

    4) Product Images from "PARP1 enhances inflammatory cytokine expression by alteration of promoter chromatin structure in microglia"

    Article Title: PARP1 enhances inflammatory cytokine expression by alteration of promoter chromatin structure in microglia

    Journal: Brain and Behavior

    doi: 10.1002/brb3.239

    LPS stimulation leads to PARP1 enzymatic activation and histone ADP-ribosylation in microglia. (A) BV2 microglial cells were stimulated with LPS for the indicated times and subsequently subjected to the PARP enzymatic activity assay. Protein was extracted with nonionic detergent and analyzed by NuPAGE, autoradiography and immunoblot (A, C and E). (B) Graphical representation of three experiments as in A. Bars indicate SEM (C) BV2 microglial cells were stimulated with LPS for the indicated times and subjected to the PARP enzymatic activity assay. Histones were isolated by acid extraction of nuclei and analyzed as in A. PJ34 (10 μ mol/L) was added 1 h prior to stimulation (lane 5). (D) Graphical representation of three experiments as in C. Bars indicate SEM (E) LPS-stimulated BV2 microglia were subjected to biochemical fractionation and the chromatin fraction was digested with MNase. Digested chromatin was layered on a 10–40% sucrose gradient and centrifuged overnight at 137,000 g . Equal volumes of each fraction were analyzed. (F) rPARP1 and rPARP2 were incubated in the presence of assembled nucleosomes at a molar ratio of 2:1 in the presence of 100 nM 32 P-NAD + . Reactions were analyzed by NuPAGE and autoradiography.
    Figure Legend Snippet: LPS stimulation leads to PARP1 enzymatic activation and histone ADP-ribosylation in microglia. (A) BV2 microglial cells were stimulated with LPS for the indicated times and subsequently subjected to the PARP enzymatic activity assay. Protein was extracted with nonionic detergent and analyzed by NuPAGE, autoradiography and immunoblot (A, C and E). (B) Graphical representation of three experiments as in A. Bars indicate SEM (C) BV2 microglial cells were stimulated with LPS for the indicated times and subjected to the PARP enzymatic activity assay. Histones were isolated by acid extraction of nuclei and analyzed as in A. PJ34 (10 μ mol/L) was added 1 h prior to stimulation (lane 5). (D) Graphical representation of three experiments as in C. Bars indicate SEM (E) LPS-stimulated BV2 microglia were subjected to biochemical fractionation and the chromatin fraction was digested with MNase. Digested chromatin was layered on a 10–40% sucrose gradient and centrifuged overnight at 137,000 g . Equal volumes of each fraction were analyzed. (F) rPARP1 and rPARP2 were incubated in the presence of assembled nucleosomes at a molar ratio of 2:1 in the presence of 100 nM 32 P-NAD + . Reactions were analyzed by NuPAGE and autoradiography.

    Techniques Used: Activation Assay, Enzyme Activity Assay, Autoradiography, Isolation, Fractionation, Incubation

    Related Articles

    Sonication:

    Article Title: Interaction of the Warsaw breakage syndrome DNA helicase DDX11 with the replication fork-protection factor Timeless promotes sister chromatid cohesion
    Article Snippet: .. Samples were subjected to sonication on ice using a Branson digital sonifier model SSE-1 (10 cycles consisting of 10-s impulses at an output 10% followed by 20-s intervals) followed by incubation for 20 min at 37°C in the presence of micrococcal nuclease (2 units/sample; Sigma, cat. N3755) and CaCl2 (at 10 mM). .. Insoluble material was removed by centrifugation at 16,000 g for 30 min.

    Incubation:

    Article Title: Centromere DNA Destabilizes H3 Nucleosomes to Promote CENP-A Deposition during the Cell Cycle
    Article Snippet: .. Chromatin was solubilized by incubation with 2 units of Micrococcal nuclease (Sigma, N3755) for 10 min at 37°C. ..

    Article Title: Interaction of the Warsaw breakage syndrome DNA helicase DDX11 with the replication fork-protection factor Timeless promotes sister chromatid cohesion
    Article Snippet: .. Samples were subjected to sonication on ice using a Branson digital sonifier model SSE-1 (10 cycles consisting of 10-s impulses at an output 10% followed by 20-s intervals) followed by incubation for 20 min at 37°C in the presence of micrococcal nuclease (2 units/sample; Sigma, cat. N3755) and CaCl2 (at 10 mM). .. Insoluble material was removed by centrifugation at 16,000 g for 30 min.

    Purification:

    Article Title: DNA-PK is activated by nucleosomes and phosphorylates H2AX within the nucleosomes in an acetylation-dependent manner
    Article Snippet: .. To prepare hyperacetylated oligonucleosomes, the chromatin of butyrate-treated HeLa cells was digested with 3 U/ml of micrococcal nuclease (Sigma), and hyperacetylated oligonucleosomes were Mg2+ -fractionated as previously described , and subjected to a glycerol gradient purification. .. The extent of acetylation of purified oligonucleosomes was determined by acid urea gel analysis and western blot with specific antibodies.

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  • 98
    Millipore micrococcal nuclease mnase
    Micrococcal Nuclease Mnase, supplied by Millipore, used in various techniques. Bioz Stars score: 98/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/micrococcal nuclease mnase/product/Millipore
    Average 98 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    micrococcal nuclease mnase - by Bioz Stars, 2020-05
    98/100 stars
      Buy from Supplier

    85
    Millipore mnase digested chip dna
    Scheme of the EBV genome and experimental design for the analyses of pre-RC and SNS zones as well as mapping of MR profiles. (A) Scheme of the circular EBV genome. In addition to the latent origins oriP (blue box) and the 14-kbp “Raji origin” (blue line), the lytic origin (oriLyt) is shown. The latent EBV nuclear antigens 1, 2, 3A–C, EBNA-LP genes (turquoise), and LMP1 and -2 (purple) are depicted, including their transcripts and promoters. The EBER1 and -2 and the miRNAs regions (BART and BARF) are indicated (green lines). The Raji genome harbors two deletions (red Δ, nt 86,000–89,000 and 163,978–166,635). These regions do not produce array signals in comparison to the reference strain type I used for the design of the EBV microarray. (B) Chart of the experimental set up to map pre-RC zones (left), <t>MNase</t> profiles (central), and SNS <t>DNA</t> (right). (C) Cell cycle phases of logarithmically growing Raji cells were separated by centrifugal elutriation. The DNA content of the different fractions was determined by FACS analysis (top, I–VI). The FACS profiles of one out of three experiments are shown. The quality of coprecipitated DNA was determined by quantitative PCR. The histograms show the mean values of three independent Orc2 (bottom left) and Mcm3 (bottom right) immunoprecipitations. The enrichments of Orc2 (red bars) and Mcm3 enrichments (blue bars) at the DS region are shown. The black bars indicate the enrichments of Orc2 and Mcm3 to a reference site. Error bars indicate mean ± SEM.
    Mnase Digested Chip Dna, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mnase digested chip dna/product/Millipore
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mnase digested chip dna - by Bioz Stars, 2020-05
    85/100 stars
      Buy from Supplier

    98
    Millipore mnase
    Scheme of the EBV genome and experimental design for the analyses of pre-RC and SNS zones as well as mapping of MR profiles. (A) Scheme of the circular EBV genome. In addition to the latent origins oriP (blue box) and the 14-kbp “Raji origin” (blue line), the lytic origin (oriLyt) is shown. The latent EBV nuclear antigens 1, 2, 3A–C, EBNA-LP genes (turquoise), and LMP1 and -2 (purple) are depicted, including their transcripts and promoters. The EBER1 and -2 and the miRNAs regions (BART and BARF) are indicated (green lines). The Raji genome harbors two deletions (red Δ, nt 86,000–89,000 and 163,978–166,635). These regions do not produce array signals in comparison to the reference strain type I used for the design of the EBV microarray. (B) Chart of the experimental set up to map pre-RC zones (left), <t>MNase</t> profiles (central), and SNS <t>DNA</t> (right). (C) Cell cycle phases of logarithmically growing Raji cells were separated by centrifugal elutriation. The DNA content of the different fractions was determined by FACS analysis (top, I–VI). The FACS profiles of one out of three experiments are shown. The quality of coprecipitated DNA was determined by quantitative PCR. The histograms show the mean values of three independent Orc2 (bottom left) and Mcm3 (bottom right) immunoprecipitations. The enrichments of Orc2 (red bars) and Mcm3 enrichments (blue bars) at the DS region are shown. The black bars indicate the enrichments of Orc2 and Mcm3 to a reference site. Error bars indicate mean ± SEM.
    Mnase, supplied by Millipore, used in various techniques. Bioz Stars score: 98/100, based on 93 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mnase/product/Millipore
    Average 98 stars, based on 93 article reviews
    Price from $9.99 to $1999.99
    mnase - by Bioz Stars, 2020-05
    98/100 stars
      Buy from Supplier

    Image Search Results


    Scheme of the EBV genome and experimental design for the analyses of pre-RC and SNS zones as well as mapping of MR profiles. (A) Scheme of the circular EBV genome. In addition to the latent origins oriP (blue box) and the 14-kbp “Raji origin” (blue line), the lytic origin (oriLyt) is shown. The latent EBV nuclear antigens 1, 2, 3A–C, EBNA-LP genes (turquoise), and LMP1 and -2 (purple) are depicted, including their transcripts and promoters. The EBER1 and -2 and the miRNAs regions (BART and BARF) are indicated (green lines). The Raji genome harbors two deletions (red Δ, nt 86,000–89,000 and 163,978–166,635). These regions do not produce array signals in comparison to the reference strain type I used for the design of the EBV microarray. (B) Chart of the experimental set up to map pre-RC zones (left), MNase profiles (central), and SNS DNA (right). (C) Cell cycle phases of logarithmically growing Raji cells were separated by centrifugal elutriation. The DNA content of the different fractions was determined by FACS analysis (top, I–VI). The FACS profiles of one out of three experiments are shown. The quality of coprecipitated DNA was determined by quantitative PCR. The histograms show the mean values of three independent Orc2 (bottom left) and Mcm3 (bottom right) immunoprecipitations. The enrichments of Orc2 (red bars) and Mcm3 enrichments (blue bars) at the DS region are shown. The black bars indicate the enrichments of Orc2 and Mcm3 to a reference site. Error bars indicate mean ± SEM.

    Journal: The Journal of Cell Biology

    Article Title: Open chromatin structures regulate the efficiencies of pre-RC formation and replication initiation in Epstein-Barr virus

    doi: 10.1083/jcb.201109105

    Figure Lengend Snippet: Scheme of the EBV genome and experimental design for the analyses of pre-RC and SNS zones as well as mapping of MR profiles. (A) Scheme of the circular EBV genome. In addition to the latent origins oriP (blue box) and the 14-kbp “Raji origin” (blue line), the lytic origin (oriLyt) is shown. The latent EBV nuclear antigens 1, 2, 3A–C, EBNA-LP genes (turquoise), and LMP1 and -2 (purple) are depicted, including their transcripts and promoters. The EBER1 and -2 and the miRNAs regions (BART and BARF) are indicated (green lines). The Raji genome harbors two deletions (red Δ, nt 86,000–89,000 and 163,978–166,635). These regions do not produce array signals in comparison to the reference strain type I used for the design of the EBV microarray. (B) Chart of the experimental set up to map pre-RC zones (left), MNase profiles (central), and SNS DNA (right). (C) Cell cycle phases of logarithmically growing Raji cells were separated by centrifugal elutriation. The DNA content of the different fractions was determined by FACS analysis (top, I–VI). The FACS profiles of one out of three experiments are shown. The quality of coprecipitated DNA was determined by quantitative PCR. The histograms show the mean values of three independent Orc2 (bottom left) and Mcm3 (bottom right) immunoprecipitations. The enrichments of Orc2 (red bars) and Mcm3 enrichments (blue bars) at the DS region are shown. The black bars indicate the enrichments of Orc2 and Mcm3 to a reference site. Error bars indicate mean ± SEM.

    Article Snippet: Southern blotting 500 ng of sonicated and MNase-digested ChIP DNA or nascent strand DNA were separated on a 1.0% TAE gel and transferred to membrane (Immobilon Ny+; EMD Millipore).

    Techniques: Microarray, FACS, Real-time Polymerase Chain Reaction