Journal: Journal of Clinical Microbiology

Article Title: Proteome microarray-guided identification of mycobacterial antigens and ELISA-based peptide mapping for improved serological detection of Mycobacterium bovis infection in European badgers

doi: 10.1128/jcm.01260-25

Figure Lengend Snippet: Mean microarray antigen spot intensities from TB-free badger serum samples at two time points following experimental infection. White bars represent TB-free sera; gray bars represent TB-infected sera collected at ( a ) 8 weeks and ( b ) 12–13 weeks post-infection. Mean microarray spot intensities (log 2 -normalized) are shown with 95% confidence interval error bars. Paired-sample t -tests were carried out comparing pre- and post-infection mean spot intensities. Antigens were ranked by BH-adjusted P -values, and the 10 most differential antigens (defined as those with the lowest BH-adjusted P -values) for each comparison group are shown. Significance levels: ns: P ≥ 0.05; *: P < 0.05; **: P < 0.01; and ***: P < 0.001.

Article Snippet: Translated proteins and IVTT controls were printed onto custom nitrocellulose-coated microarray slides (Grace Bio-Labs, Bend, OR, USA) using a GeneMachines OmniGrid Accent Microarray Printer.

Techniques: Microarray, Infection, Comparison

Journal: Journal of Clinical Microbiology

Article Title: Proteome microarray-guided identification of mycobacterial antigens and ELISA-based peptide mapping for improved serological detection of Mycobacterium bovis infection in European badgers

doi: 10.1128/jcm.01260-25

Figure Lengend Snippet: Mean microarray antigen spot intensities from TB-infected and BCG-vaccinated badger serum samples. Gray bars represent sera from TB-infected badgers collected 12–13 weeks after experimental infection; white bars represent sera from badgers that received ( a ) a single BCG vaccination or ( b ) two BCG vaccinations. Mean spot intensities (log₂-normalized) are shown with 95% confidence interval error bars. Independent-samples t -tests were used to compare antigen intensities between TB-infected and BCG-vaccinated groups. Antigens were ranked by BH-adjusted P -values, and the 10 most differential antigens (defined as those with the lowest BH-adjusted P -values) for each comparison group are shown; ***: P < 0.001.

Article Snippet: Translated proteins and IVTT controls were printed onto custom nitrocellulose-coated microarray slides (Grace Bio-Labs, Bend, OR, USA) using a GeneMachines OmniGrid Accent Microarray Printer.

Techniques: Microarray, Infection, Comparison

Journal: Cell Death Discovery

Article Title: Targeting GPX2 to disrupt lipid homeostasis and enhance cisplatin sensitivity in diffuse gastric cancer

doi: 10.1038/s41420-025-02771-8

Figure Lengend Snippet: A Analysis of GPX2 expression in normal versus tumor tissues using the TCGA-STAD and GTEx databases. B GPX2 expression in normal and tumor tissues from the ACRG dataset. C Determination of GPX2 protein levels in gastric cancer cell lines via Western blot analysis. D Measurement of GPX2 protein levels in tumor tissues and adjacent non-tumor tissues from DGC patients using Western blot. E Immunohistochemical staining of GPX2 in tissue microarray composed of cancerous and adjacent non-cancerous tissues from 160 gastric cancer patients. Scale bars = 50 μm or 200 μm. F Distribution of the difference in immunohistochemistry (IHC) scores (ΔIRS = IRST–IRSN) for GPX2 in tissue microarrays. G Kaplan–Meier survival curves for gastric cancer patients with high and low GPX2 expression. H Univariate Cox regression analysis assessing the prognostic significance of GPX2 expression. I Time-dependent AUC curve of GPX2.

Article Snippet: The microarray slides were processed and stained by Servicebio (Wuhan, China), following a standardized staining protocol.

Techniques: Expressing, Western Blot, Immunohistochemical staining, Staining, Microarray, Immunohistochemistry