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Comprehensive <t>microarray</t> polymer profiling (CoMPP) from Arabidopsis thaliana wild type and ARAK1‐OE seedlings grown on 3 mM L‐Ara. Analysis of cell wall composition by carbohydrate profiling is shown. Alcohol‐insoluble residue (AIR) samples were isolated from roots of wild type (WT) and ARAK1‐OE mutants growing for 14 days on 0.5× MS‐agar plates supplemented with 3 mM L‐Ara. Cell wall components were extracted from AIR samples with diaminocyclo‐hexane‐tetra‐acetic acid (CDTA) and NaOH. Blue colors indicate increased intensity of respective antibody binding according to the legend.
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Comprehensive <t>microarray</t> polymer profiling (CoMPP) from Arabidopsis thaliana wild type and ARAK1‐OE seedlings grown on 3 mM L‐Ara. Analysis of cell wall composition by carbohydrate profiling is shown. Alcohol‐insoluble residue (AIR) samples were isolated from roots of wild type (WT) and ARAK1‐OE mutants growing for 14 days on 0.5× MS‐agar plates supplemented with 3 mM L‐Ara. Cell wall components were extracted from AIR samples with diaminocyclo‐hexane‐tetra‐acetic acid (CDTA) and NaOH. Blue colors indicate increased intensity of respective antibody binding according to the legend.
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Comprehensive <t>microarray</t> polymer profiling (CoMPP) from Arabidopsis thaliana wild type and ARAK1‐OE seedlings grown on 3 mM L‐Ara. Analysis of cell wall composition by carbohydrate profiling is shown. Alcohol‐insoluble residue (AIR) samples were isolated from roots of wild type (WT) and ARAK1‐OE mutants growing for 14 days on 0.5× MS‐agar plates supplemented with 3 mM L‐Ara. Cell wall components were extracted from AIR samples with diaminocyclo‐hexane‐tetra‐acetic acid (CDTA) and NaOH. Blue colors indicate increased intensity of respective antibody binding according to the legend.
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Comprehensive <t>microarray</t> polymer profiling (CoMPP) from Arabidopsis thaliana wild type and ARAK1‐OE seedlings grown on 3 mM L‐Ara. Analysis of cell wall composition by carbohydrate profiling is shown. Alcohol‐insoluble residue (AIR) samples were isolated from roots of wild type (WT) and ARAK1‐OE mutants growing for 14 days on 0.5× MS‐agar plates supplemented with 3 mM L‐Ara. Cell wall components were extracted from AIR samples with diaminocyclo‐hexane‐tetra‐acetic acid (CDTA) and NaOH. Blue colors indicate increased intensity of respective antibody binding according to the legend.
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Comprehensive <t>microarray</t> polymer profiling (CoMPP) from Arabidopsis thaliana wild type and ARAK1‐OE seedlings grown on 3 mM L‐Ara. Analysis of cell wall composition by carbohydrate profiling is shown. Alcohol‐insoluble residue (AIR) samples were isolated from roots of wild type (WT) and ARAK1‐OE mutants growing for 14 days on 0.5× MS‐agar plates supplemented with 3 mM L‐Ara. Cell wall components were extracted from AIR samples with diaminocyclo‐hexane‐tetra‐acetic acid (CDTA) and NaOH. Blue colors indicate increased intensity of respective antibody binding according to the legend.
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microarray printer sciflexarrayer s12  (SCIENION)
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Comprehensive <t>microarray</t> polymer profiling (CoMPP) from Arabidopsis thaliana wild type and ARAK1‐OE seedlings grown on 3 mM L‐Ara. Analysis of cell wall composition by carbohydrate profiling is shown. Alcohol‐insoluble residue (AIR) samples were isolated from roots of wild type (WT) and ARAK1‐OE mutants growing for 14 days on 0.5× MS‐agar plates supplemented with 3 mM L‐Ara. Cell wall components were extracted from AIR samples with diaminocyclo‐hexane‐tetra‐acetic acid (CDTA) and NaOH. Blue colors indicate increased intensity of respective antibody binding according to the legend.
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Comprehensive <t>microarray</t> polymer profiling (CoMPP) from Arabidopsis thaliana wild type and ARAK1‐OE seedlings grown on 3 mM L‐Ara. Analysis of cell wall composition by carbohydrate profiling is shown. Alcohol‐insoluble residue (AIR) samples were isolated from roots of wild type (WT) and ARAK1‐OE mutants growing for 14 days on 0.5× MS‐agar plates supplemented with 3 mM L‐Ara. Cell wall components were extracted from AIR samples with diaminocyclo‐hexane‐tetra‐acetic acid (CDTA) and NaOH. Blue colors indicate increased intensity of respective antibody binding according to the legend.
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sciflexarrayer s3 microarray printer  (SCIENION)
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Comprehensive <t>microarray</t> polymer profiling (CoMPP) from Arabidopsis thaliana wild type and ARAK1‐OE seedlings grown on 3 mM L‐Ara. Analysis of cell wall composition by carbohydrate profiling is shown. Alcohol‐insoluble residue (AIR) samples were isolated from roots of wild type (WT) and ARAK1‐OE mutants growing for 14 days on 0.5× MS‐agar plates supplemented with 3 mM L‐Ara. Cell wall components were extracted from AIR samples with diaminocyclo‐hexane‐tetra‐acetic acid (CDTA) and NaOH. Blue colors indicate increased intensity of respective antibody binding according to the legend.
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nanoprint® microarray printer  (Arrayit Corporation)
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Arrayit Corporation nanoprint® microarray printer
Comprehensive <t>microarray</t> polymer profiling (CoMPP) from Arabidopsis thaliana wild type and ARAK1‐OE seedlings grown on 3 mM L‐Ara. Analysis of cell wall composition by carbohydrate profiling is shown. Alcohol‐insoluble residue (AIR) samples were isolated from roots of wild type (WT) and ARAK1‐OE mutants growing for 14 days on 0.5× MS‐agar plates supplemented with 3 mM L‐Ara. Cell wall components were extracted from AIR samples with diaminocyclo‐hexane‐tetra‐acetic acid (CDTA) and NaOH. Blue colors indicate increased intensity of respective antibody binding according to the legend.
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Comprehensive microarray polymer profiling (CoMPP) from Arabidopsis thaliana wild type and ARAK1‐OE seedlings grown on 3 mM L‐Ara. Analysis of cell wall composition by carbohydrate profiling is shown. Alcohol‐insoluble residue (AIR) samples were isolated from roots of wild type (WT) and ARAK1‐OE mutants growing for 14 days on 0.5× MS‐agar plates supplemented with 3 mM L‐Ara. Cell wall components were extracted from AIR samples with diaminocyclo‐hexane‐tetra‐acetic acid (CDTA) and NaOH. Blue colors indicate increased intensity of respective antibody binding according to the legend.

Journal: Plant Direct

Article Title: Arabinokinase Limits the Flux of Arabinose Into Nucleotide Sugars to Prevent Toxicity

doi: 10.1002/pld3.70094

Figure Lengend Snippet: Comprehensive microarray polymer profiling (CoMPP) from Arabidopsis thaliana wild type and ARAK1‐OE seedlings grown on 3 mM L‐Ara. Analysis of cell wall composition by carbohydrate profiling is shown. Alcohol‐insoluble residue (AIR) samples were isolated from roots of wild type (WT) and ARAK1‐OE mutants growing for 14 days on 0.5× MS‐agar plates supplemented with 3 mM L‐Ara. Cell wall components were extracted from AIR samples with diaminocyclo‐hexane‐tetra‐acetic acid (CDTA) and NaOH. Blue colors indicate increased intensity of respective antibody binding according to the legend.

Article Snippet: Arrays were printed as distinct dots onto nitrocellulose membranes with an ArrayJet Sprint microarray printer (ArrayJet, Roslin, UK) and probed with ~40 cell wall probes, including antibodies and carbohydrate binding modules (Table ).

Techniques: Microarray, Polymer, Residue, Isolation, Binding Assay