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electrode array microarray format  (CombiMatrix)

 
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    CombiMatrix electrode array microarray format
    (A) Representative images of an aptamer <t>microarray</t> incubated with labeled IgE. The eight identical subarrays were individually yet simultaneously interrogated with decreasing concentrations of IgE (100 nM to 0.1 nM). Insets (30 nM IgE) demonstrate discrete features of uniform size and intensity. (B,C) Subarrays incubated with 10 nM IgE from two separate experiments were used to analyze intra- and inter-slide data correlation (B and C, respectively). Both correlation analyses demonstrated a high degree of reproducibility, with r 2 values greater than 0.990. The slope of the correlation trend is indicative of relative fluorescence intensities. Slide 2 was washed less stringently than slide 1, hence the higher fluorescence intensities of its features are reflected in a slope of 1.84 (vs. 1.04 in the intraslide comparison where all wash conditions were constant). Averaging of triplicate data points (c, red circles) effectively eliminated outliers seen in the plot of individual data points (black circles). Average percent coefficient of variability (%CV) values for intra- and interslide subarray comparisons were determined to be 5.76% and 6.86%, respectively (see ).
    Electrode Array Microarray Format, supplied by CombiMatrix, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/microarray+electrodes/microelectrode+array/pmc02444025-135-3-2
    Average 90 stars, based on 1 article reviews
    electrode array microarray format - by Bioz Stars, 2026-10
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    Images

    1) Product Images from "Massively Parallel Interrogation of Aptamer Sequence, Structure and Function"

    Article Title: Massively Parallel Interrogation of Aptamer Sequence, Structure and Function

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0002720

    (A) Representative images of an aptamer microarray incubated with labeled IgE. The eight identical subarrays were individually yet simultaneously interrogated with decreasing concentrations of IgE (100 nM to 0.1 nM). Insets (30 nM IgE) demonstrate discrete features of uniform size and intensity. (B,C) Subarrays incubated with 10 nM IgE from two separate experiments were used to analyze intra- and inter-slide data correlation (B and C, respectively). Both correlation analyses demonstrated a high degree of reproducibility, with r 2 values greater than 0.990. The slope of the correlation trend is indicative of relative fluorescence intensities. Slide 2 was washed less stringently than slide 1, hence the higher fluorescence intensities of its features are reflected in a slope of 1.84 (vs. 1.04 in the intraslide comparison where all wash conditions were constant). Averaging of triplicate data points (c, red circles) effectively eliminated outliers seen in the plot of individual data points (black circles). Average percent coefficient of variability (%CV) values for intra- and interslide subarray comparisons were determined to be 5.76% and 6.86%, respectively (see ).
    Figure Legend Snippet: (A) Representative images of an aptamer microarray incubated with labeled IgE. The eight identical subarrays were individually yet simultaneously interrogated with decreasing concentrations of IgE (100 nM to 0.1 nM). Insets (30 nM IgE) demonstrate discrete features of uniform size and intensity. (B,C) Subarrays incubated with 10 nM IgE from two separate experiments were used to analyze intra- and inter-slide data correlation (B and C, respectively). Both correlation analyses demonstrated a high degree of reproducibility, with r 2 values greater than 0.990. The slope of the correlation trend is indicative of relative fluorescence intensities. Slide 2 was washed less stringently than slide 1, hence the higher fluorescence intensities of its features are reflected in a slope of 1.84 (vs. 1.04 in the intraslide comparison where all wash conditions were constant). Averaging of triplicate data points (c, red circles) effectively eliminated outliers seen in the plot of individual data points (black circles). Average percent coefficient of variability (%CV) values for intra- and interslide subarray comparisons were determined to be 5.76% and 6.86%, respectively (see ).

    Techniques Used: Microarray, Incubation, Labeling, Fluorescence

    Related Articles

    Microarray:

    Article Title: Microarray having a base cleavable linker
    Article Snippet: .. An example of an electrode microarray is a CombiMatrix CustomArrayTM 12 k, which has over 12,000 electrodes and an electrode density of approximately 17,778 electrodes per square centimeter. ..

    Article Title: Microarray having a base cleavable linker
    Article Snippet: .. An example of an electrode microarray is a CombiMatrix CustomArrayTM 12K, which has over 12,000 electrodes and an electrode density of approximately 17,778 electrodes per square centimeter. ..

    Article Title: Process and apparatus for measuring binding events on a microarray of electrodes
    Article Snippet: Preferably, the electrode microarray is a CombiMatrix Corporation CUSTOMARRAY12KTM (approximately 12,000 electrodes per square centimeter). .. Alternatively, the electrode microarray is a CombiMatrix Corporation CUSTOMARRAY902TM (approximately 1,000 electrodes per square centimeter). .. Alternatively, the electrode microarray is a CombiMatrix Corporation CUSTOMARRAY902TM (approximately 1,000 electrodes per square centimeter).

    Article Title: Electrochemical deblocking using a hydrazine derivative
    Article Snippet: .. In this example, the electrode microarray used was a commercial microarray, CombiMatrix CustomArray. ..

    Article Title: Massively Parallel Interrogation of Aptamer Sequence, Structure and Function
    Article Snippet: .. Indeed, the Combimatrix electrode array microarray format has the potential to be used much like the Agilent arrays were used here but with an electronic readout. ..

    Article Title: Advances in Molecular Diagnostic Approaches for Biothreat Agents
    Article Snippet: .. The integrated multiplexed microarray biosensor CombiMatrix ElectraSense microarray detects Y. pestis , B. anthracis , and SEB. ..

    Article Title: Enzyme-amplified redox microarray detection process
    Article Snippet: .. Specifically, FIG. 12 shows amperometric detection of rabbit IgG bound to selected electrodes on a microarray biochip containing electrodes and a polyvinyl alcohol porous matrix (Combimatrix Corporation, Mukilteo, Wash.). ..



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    Image Search Results


    (A) Representative images of an aptamer microarray incubated with labeled IgE. The eight identical subarrays were individually yet simultaneously interrogated with decreasing concentrations of IgE (100 nM to 0.1 nM). Insets (30 nM IgE) demonstrate discrete features of uniform size and intensity. (B,C) Subarrays incubated with 10 nM IgE from two separate experiments were used to analyze intra- and inter-slide data correlation (B and C, respectively). Both correlation analyses demonstrated a high degree of reproducibility, with r 2 values greater than 0.990. The slope of the correlation trend is indicative of relative fluorescence intensities. Slide 2 was washed less stringently than slide 1, hence the higher fluorescence intensities of its features are reflected in a slope of 1.84 (vs. 1.04 in the intraslide comparison where all wash conditions were constant). Averaging of triplicate data points (c, red circles) effectively eliminated outliers seen in the plot of individual data points (black circles). Average percent coefficient of variability (%CV) values for intra- and interslide subarray comparisons were determined to be 5.76% and 6.86%, respectively (see ).

    Journal: PLoS ONE

    Article Title: Massively Parallel Interrogation of Aptamer Sequence, Structure and Function

    doi: 10.1371/journal.pone.0002720

    Figure Lengend Snippet: (A) Representative images of an aptamer microarray incubated with labeled IgE. The eight identical subarrays were individually yet simultaneously interrogated with decreasing concentrations of IgE (100 nM to 0.1 nM). Insets (30 nM IgE) demonstrate discrete features of uniform size and intensity. (B,C) Subarrays incubated with 10 nM IgE from two separate experiments were used to analyze intra- and inter-slide data correlation (B and C, respectively). Both correlation analyses demonstrated a high degree of reproducibility, with r 2 values greater than 0.990. The slope of the correlation trend is indicative of relative fluorescence intensities. Slide 2 was washed less stringently than slide 1, hence the higher fluorescence intensities of its features are reflected in a slope of 1.84 (vs. 1.04 in the intraslide comparison where all wash conditions were constant). Averaging of triplicate data points (c, red circles) effectively eliminated outliers seen in the plot of individual data points (black circles). Average percent coefficient of variability (%CV) values for intra- and interslide subarray comparisons were determined to be 5.76% and 6.86%, respectively (see ).

    Article Snippet: Indeed, the Combimatrix electrode array microarray format has the potential to be used much like the Agilent arrays were used here but with an electronic readout.

    Techniques: Microarray, Incubation, Labeling, Fluorescence