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microarray data analysis software expressionist  (Genedata Inc)
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Genedata Inc microarray data analysis software expressionist
Genes showing differential expression between FDKs from donors with atopic dermatitis (AD) and from non-atopic controls (Non-AD) by <t>microarray</t> analysis, as determined by comparison analysis using a permutation test. The log 2 ratio (the Cy5 to Cy3 fluorescence intensity using the same Cy3-labelled probe, consisting of a mixture of NHEK extracts and Universal Human Reference RNA, as a reference) was used as a expression level. For comparative studies of gene expression between AD-FDKs and Non-AD-FDKs, a permutation test was performed (number of permutations: 500) and 827 genes were extracted. In this figure, 11 genes that exhibit a difference of at least 2-fold and meet the criterion p < 0.05 are visualized as the tree after hierarchical clustering (Distance; pearson uncentered, Linkage; complete).
Microarray Data Analysis Software Expressionist, supplied by Genedata Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Genes showing differential expression between FDKs from donors with atopic dermatitis (AD) and from non-atopic controls (Non-AD) by microarray analysis, as determined by comparison analysis using a permutation test. The log 2 ratio (the Cy5 to Cy3 fluorescence intensity using the same Cy3-labelled probe, consisting of a mixture of NHEK extracts and Universal Human Reference RNA, as a reference) was used as a expression level. For comparative studies of gene expression between AD-FDKs and Non-AD-FDKs, a permutation test was performed (number of permutations: 500) and 827 genes were extracted. In this figure, 11 genes that exhibit a difference of at least 2-fold and meet the criterion p < 0.05 are visualized as the tree after hierarchical clustering (Distance; pearson uncentered, Linkage; complete).

Journal: International Journal of Molecular Sciences

Article Title: Transcriptional Analysis of Hair Follicle-Derived Keratinocytes from Donors with Atopic Dermatitis Reveals Enhanced Induction of IL32 Gene by IFN-γ

doi: 10.3390/ijms14023215

Figure Lengend Snippet: Genes showing differential expression between FDKs from donors with atopic dermatitis (AD) and from non-atopic controls (Non-AD) by microarray analysis, as determined by comparison analysis using a permutation test. The log 2 ratio (the Cy5 to Cy3 fluorescence intensity using the same Cy3-labelled probe, consisting of a mixture of NHEK extracts and Universal Human Reference RNA, as a reference) was used as a expression level. For comparative studies of gene expression between AD-FDKs and Non-AD-FDKs, a permutation test was performed (number of permutations: 500) and 827 genes were extracted. In this figure, 11 genes that exhibit a difference of at least 2-fold and meet the criterion p < 0.05 are visualized as the tree after hierarchical clustering (Distance; pearson uncentered, Linkage; complete).

Article Snippet: Probes with poor-quality signals (signal/noise < 1.2) and those with null data at a frequency of more than 35% in all samples were filtered out using the microarray data analysis software Expressionist (GeneData AG, Basel, Switzerland); after this procedure, 13,722 probes remained.

Techniques: Quantitative Proteomics, Microarray, Comparison, Fluorescence, Expressing, Gene Expression