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whole mouse genome microarray comparisons  (Agilent technologies)


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    Agilent technologies whole mouse genome microarray comparisons
    Whole Mouse Genome Microarray Comparisons, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/microarray+comparison/pm21320871-199-1-0
    Average 86 stars, based on 1 article reviews
    whole mouse genome microarray comparisons - by Bioz Stars, 2026-09
    86/100 stars

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    other:

    Article Title: Mass Spectrometry Imaging Reveals Abnormalities in Cardiolipin Composition and Distribution in Astrocytoma Tumor Tissues.
    Article Snippet: Comparison of expression levels was restricted to the following primary adult tumors from the CGGA dataset [32] containing mRNA microarray data collected on the Agilent Whole Human Genome (Array) platform: astrocytoma, anaplastic astrocytoma, and glioblastoma.

    Article Title: Identification of Molecular Markers Associated with Prostate Cancer Subtypes: An Integrative Bioinformatics Approach
    Article Snippet: In particular, the comparison of metastatic versus primary PCa and mCRPC versus primary PCa have been performed using the following 8 datasets from Gene Expression Omnibus (GEO) portal: GSE3325 (expression profiling by array: Platform GLP570 Affymetrix Human Genome U133 Plus 2.0 Array) [ ], GSE3933 (expression profiling by array: Platform GPL2695) [ ], GSE68882 (GPL91 [HG_U95A] Affymetrix Human Genome U95A Array) [ ], GSE32269 (GPL96 [HG-U133A] Affymetrix Human Genome U133A Array) [ ], GSE6811 (GPL4747YN Human 36 K (sets 1–8)) [ ], GSE70770 (GPL10558 Illumina HumanHT-12 V4.0 expression beadchip) [ ], GSE6752 (GPL2891 GE Healthcare/Amersham Biosciences CodeLinkTM UniSet Human 20 K I Bioarray) [ ] and GSE35988 (GPL9075 Agilent-014698 Human Genome CGH Microarray 105A (G4412A)) [ ].

    Article Title: Prenatal Alcohol Exposure Impairs the Placenta–Cortex Transcriptomic Signature, Leading to Dysregulation of Angiogenic Pathways
    Article Snippet: One-color comparative hybridization was performed using Whole Mouse Genome Oligo 4_44K Microarray (G2519F-014868, Agilent Technologies, Les Ulis, France) to compare gene expression profiling. cRNAs were synthesized from 100 ng total RNA, labeled using a Quick Amp Labeling Kit (Agilent Technologies), and hybridized on microarrays at 65 °C for 17 h. Raw hybridization data, evaluated on every probe 5 μm-sized array, using a DNA microarray scanner G2565CA (Agilent Technologies), were extracted with Feature Extraction Software 10.5.1.1 (Agilent Technologies), then transferred to Genespring ® (GX 12.6 software, Agilent Technologies) for data processing (normalization) and data mining ( B).

    Microarray:

    Article Title: Deciphering potential vascularization factors of on-chip co-cultured hiPSC-derived cerebral organoids.
    Article Snippet: The dye incorporation efficiency was detected using a NanoDrop One/One spectrophotometer (Thermo Fisher Scientific). .. Agilent whole human genome microarray comparisons of CO outer layers were performed for co-cultured organoids prior to detecting the vascular bed angiogenic response (d28, control), during visible angiogenic response (d31), and inhibited angiogenesis (d35), producing two independent comparison groups: d31 (angiogenesis) versus d28 (control) and d35 (inhibited angiogenesis) versus d28 (control) (Fig. 1). ..

    Article Title: High‐grade bladder cancer cells secrete extracellular vesicles containing miRNA‐146a‐5p and promotes angiogenesis
    Article Snippet: Then, 0.60 μg of Cy3 dye‐labelled cRNA was hybridized onto a SurePrint G3 Human GE v2 8 × 60K (Agilent Technologies; design ID: 072363). .. After scanning the microarray chip using an Agilent microarray DNA scanner (G4900DA), the intensity values were quantified using Agilent Feature Extraction software version 11.5.1.1. .. Normalization was performed using Agilent GeneSpring software version 14.9.

    Article Title: Assessing the Link between Diabetic Metabolic Dysregulation and Breast Cancer Progression
    Article Snippet: Finally, the labelled cRNA samples were fragmented at 60 °C and hybridized onto an Agilent Human Gene Expression Microarray 8 × 60 K. The Gene Expression Hybridization kit (Agilent Technologies, in situ Hybridization kit, Part Number 5190-0404) fragmented the labelled cRNA. .. Next, hybridization was performed in Agilent’s Surehyb Chambers at 65 °C for 16 h, after which they were washed using Agilent Gene Expression wash buffers (Agilent Technologies, Part Number 5188-5327) and scanned using the Agilent Microarray Scanner (Agilent Technologies, Part Number G2600D). .. Agilent Feature Extraction software (v11.5.1.1) extracted raw data from the scanned images and fed them into the Agilent GeneSpring GX (v14.5) software for analysis.

    Article Title: Potential therapeutic implications of histidine catabolism by the gut microbiota in NAFLD patients with morbid obesity
    Article Snippet: The amounts of cRNA and dye that were incorporated were measured by an ND-1000 spectrophotometer (NanoDrop Technologies). .. Hybridization of the Agilent Whole Human Genome Oligo Microarrays 4 × 44K was done following the Agilent 60-mer oligo microarray processing protocol using the Agilent Gene Expression Hybridization Kit. .. The fluorescence signals of the hybridized Agilent microarrays were detected using Agilent’s Microarray Scanner after washing with Agilent Gene Expression Wash Buffer twice and with acetonitrile once.

    Article Title: Notch Is Not Involved in Physioxia-Mediated Stem Cell Maintenance in Midbrain Neural Stem Cells
    Article Snippet: Hybridi-zation to whole mouse genome microarray gene expression chips (Gene ST 1.0 Arrays, Affymetrix) was performed according to manufacturer’s instructions. .. Microarray chips were then immediately scanned using an Agilent microarray confocal laser scanner. .. Microarray data analysis was performed with the Agilent GeneSpring GX11 software.

    Control:

    Article Title: Deciphering potential vascularization factors of on-chip co-cultured hiPSC-derived cerebral organoids.
    Article Snippet: The dye incorporation efficiency was detected using a NanoDrop One/One spectrophotometer (Thermo Fisher Scientific). .. Agilent whole human genome microarray comparisons of CO outer layers were performed for co-cultured organoids prior to detecting the vascular bed angiogenic response (d28, control), during visible angiogenic response (d31), and inhibited angiogenesis (d35), producing two independent comparison groups: d31 (angiogenesis) versus d28 (control) and d35 (inhibited angiogenesis) versus d28 (control) (Fig. 1). ..

    Comparison:

    Article Title: Deciphering potential vascularization factors of on-chip co-cultured hiPSC-derived cerebral organoids.
    Article Snippet: The dye incorporation efficiency was detected using a NanoDrop One/One spectrophotometer (Thermo Fisher Scientific). .. Agilent whole human genome microarray comparisons of CO outer layers were performed for co-cultured organoids prior to detecting the vascular bed angiogenic response (d28, control), during visible angiogenic response (d31), and inhibited angiogenesis (d35), producing two independent comparison groups: d31 (angiogenesis) versus d28 (control) and d35 (inhibited angiogenesis) versus d28 (control) (Fig. 1). ..

    Extraction:

    Article Title: High‐grade bladder cancer cells secrete extracellular vesicles containing miRNA‐146a‐5p and promotes angiogenesis
    Article Snippet: Then, 0.60 μg of Cy3 dye‐labelled cRNA was hybridized onto a SurePrint G3 Human GE v2 8 × 60K (Agilent Technologies; design ID: 072363). .. After scanning the microarray chip using an Agilent microarray DNA scanner (G4900DA), the intensity values were quantified using Agilent Feature Extraction software version 11.5.1.1. .. Normalization was performed using Agilent GeneSpring software version 14.9.

    Software:

    Article Title: High‐grade bladder cancer cells secrete extracellular vesicles containing miRNA‐146a‐5p and promotes angiogenesis
    Article Snippet: Then, 0.60 μg of Cy3 dye‐labelled cRNA was hybridized onto a SurePrint G3 Human GE v2 8 × 60K (Agilent Technologies; design ID: 072363). .. After scanning the microarray chip using an Agilent microarray DNA scanner (G4900DA), the intensity values were quantified using Agilent Feature Extraction software version 11.5.1.1. .. Normalization was performed using Agilent GeneSpring software version 14.9.

    Hybridization:

    Article Title: Assessing the Link between Diabetic Metabolic Dysregulation and Breast Cancer Progression
    Article Snippet: Finally, the labelled cRNA samples were fragmented at 60 °C and hybridized onto an Agilent Human Gene Expression Microarray 8 × 60 K. The Gene Expression Hybridization kit (Agilent Technologies, in situ Hybridization kit, Part Number 5190-0404) fragmented the labelled cRNA. .. Next, hybridization was performed in Agilent’s Surehyb Chambers at 65 °C for 16 h, after which they were washed using Agilent Gene Expression wash buffers (Agilent Technologies, Part Number 5188-5327) and scanned using the Agilent Microarray Scanner (Agilent Technologies, Part Number G2600D). .. Agilent Feature Extraction software (v11.5.1.1) extracted raw data from the scanned images and fed them into the Agilent GeneSpring GX (v14.5) software for analysis.

    Article Title: Potential therapeutic implications of histidine catabolism by the gut microbiota in NAFLD patients with morbid obesity
    Article Snippet: The amounts of cRNA and dye that were incorporated were measured by an ND-1000 spectrophotometer (NanoDrop Technologies). .. Hybridization of the Agilent Whole Human Genome Oligo Microarrays 4 × 44K was done following the Agilent 60-mer oligo microarray processing protocol using the Agilent Gene Expression Hybridization Kit. .. The fluorescence signals of the hybridized Agilent microarrays were detected using Agilent’s Microarray Scanner after washing with Agilent Gene Expression Wash Buffer twice and with acetonitrile once.

    Gene Expression:

    Article Title: Assessing the Link between Diabetic Metabolic Dysregulation and Breast Cancer Progression
    Article Snippet: Finally, the labelled cRNA samples were fragmented at 60 °C and hybridized onto an Agilent Human Gene Expression Microarray 8 × 60 K. The Gene Expression Hybridization kit (Agilent Technologies, in situ Hybridization kit, Part Number 5190-0404) fragmented the labelled cRNA. .. Next, hybridization was performed in Agilent’s Surehyb Chambers at 65 °C for 16 h, after which they were washed using Agilent Gene Expression wash buffers (Agilent Technologies, Part Number 5188-5327) and scanned using the Agilent Microarray Scanner (Agilent Technologies, Part Number G2600D). .. Agilent Feature Extraction software (v11.5.1.1) extracted raw data from the scanned images and fed them into the Agilent GeneSpring GX (v14.5) software for analysis.

    Article Title: Potential therapeutic implications of histidine catabolism by the gut microbiota in NAFLD patients with morbid obesity
    Article Snippet: The amounts of cRNA and dye that were incorporated were measured by an ND-1000 spectrophotometer (NanoDrop Technologies). .. Hybridization of the Agilent Whole Human Genome Oligo Microarrays 4 × 44K was done following the Agilent 60-mer oligo microarray processing protocol using the Agilent Gene Expression Hybridization Kit. .. The fluorescence signals of the hybridized Agilent microarrays were detected using Agilent’s Microarray Scanner after washing with Agilent Gene Expression Wash Buffer twice and with acetonitrile once.



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    Image Search Results


    Confirmation of cDNA-Array data by quantitative RT-PCT

    Journal: BMC Cancer

    Article Title: Metastatic canine mammary carcinomas can be identified by a gene expression profile that partly overlaps with human breast cancer profiles

    doi: 10.1186/1471-2407-10-618

    Figure Lengend Snippet: Confirmation of cDNA-Array data by quantitative RT-PCT

    Article Snippet: To supplement the gene annotations of the differentially expressed genes with functional information, BLAST search and Affymetrix-provided human to canine microarray comparisons were used to map canine genes to their human equivalents as has been shown before [ ].

    Techniques: Microarray

    Figure 1. Study design. Carotid plaques (n=34) from asymptomatic and symptomatic patients were sectioned at 2 levels and 3 cores drilled from each level (A) to construct 2 tissue microarray (TMA) blocks with 204 cores in total (B). Based on symptomatic vs asymptom- atic microarray comparisons generated for n=127 plaques (C), highly upregulated genes were chosen for the study (D; in red). Several genes already known to be associated with atherosclerosis were also included (D; in black). TMAs were stained by immunohistochemistry (IHC) with antibodies toward human proteins of interest, and tissue sections were scored (0, 1, 2, or 3) for semiquantitative grading of staining intensity (E). Significantly differentially expressed proteins were validated further by IHC on individual plaques (F).

    Journal: Arteriosclerosis, Thrombosis, and Vascular Biology

    Article Title: Profiling of Atherosclerotic Lesions by Gene and Tissue Microarrays Reveals PCSK6 as a Novel Protease in Unstable Carotid Atherosclerosis

    doi: 10.1161/atvbaha.113.301743

    Figure Lengend Snippet: Figure 1. Study design. Carotid plaques (n=34) from asymptomatic and symptomatic patients were sectioned at 2 levels and 3 cores drilled from each level (A) to construct 2 tissue microarray (TMA) blocks with 204 cores in total (B). Based on symptomatic vs asymptom- atic microarray comparisons generated for n=127 plaques (C), highly upregulated genes were chosen for the study (D; in red). Several genes already known to be associated with atherosclerosis were also included (D; in black). TMAs were stained by immunohistochemistry (IHC) with antibodies toward human proteins of interest, and tissue sections were scored (0, 1, 2, or 3) for semiquantitative grading of staining intensity (E). Significantly differentially expressed proteins were validated further by IHC on individual plaques (F).

    Article Snippet: From the list of highly upregulated genes obtained by microarray comparisons between symptomatic and asymptomatic carotid lesions, candidates with relatively restricted tissue distribution in humans were chosen for further analysis (based on the Human Protein Atlas).

    Techniques: Construct, Microarray, Generated, Staining, Immunohistochemistry

    Figure 4. PCSK6 is the most significantly upregulated proprotein convertase (PC) in symptomatic carotid plaques. Analy- ses of n=127 microarray profiles between carotid plaques (CP) and normal controls (iliac arteries [IA]) show significant down- regulation of Furin and PCSK5 in plaque tissue, upregulation of PCSK6 and PCSK7, whereas PCSK9 and PCSK2 did not exhibit significant variation in expression (A). Posi- tive correlation was found between expres- sion of PCSK6 and PCSK9, PCSK2 and PCSK7, whereas negative was observed with PCSK5 (B). Several PCSK6 isoforms recognized by different Affymetrix probes were significantly upregulated in plaques compared with controls, but most highly the isoform coding for secreted protein (C). This isoform was the only one with signifi- cantly higher expression in comparison between symptomatic (s) and asymptom- atic (as) plaques (D). Results were con- firmed by quantitative real-time polymerase chain reaction (qRT-PCR) analyses for expression of secreted PCSK6 isoform in a separate cohort of n=233 carotid plaques (E and F). Micorarray data expressed as log2 value and qRT-PCR data as fold change compared with control. Data in A, C, and D show mean with SD, whereas median with interquartile range is depicted in E and F. ER indicates endoplasmatic reticulum.

    Journal: Arteriosclerosis, Thrombosis, and Vascular Biology

    Article Title: Profiling of Atherosclerotic Lesions by Gene and Tissue Microarrays Reveals PCSK6 as a Novel Protease in Unstable Carotid Atherosclerosis

    doi: 10.1161/atvbaha.113.301743

    Figure Lengend Snippet: Figure 4. PCSK6 is the most significantly upregulated proprotein convertase (PC) in symptomatic carotid plaques. Analy- ses of n=127 microarray profiles between carotid plaques (CP) and normal controls (iliac arteries [IA]) show significant down- regulation of Furin and PCSK5 in plaque tissue, upregulation of PCSK6 and PCSK7, whereas PCSK9 and PCSK2 did not exhibit significant variation in expression (A). Posi- tive correlation was found between expres- sion of PCSK6 and PCSK9, PCSK2 and PCSK7, whereas negative was observed with PCSK5 (B). Several PCSK6 isoforms recognized by different Affymetrix probes were significantly upregulated in plaques compared with controls, but most highly the isoform coding for secreted protein (C). This isoform was the only one with signifi- cantly higher expression in comparison between symptomatic (s) and asymptom- atic (as) plaques (D). Results were con- firmed by quantitative real-time polymerase chain reaction (qRT-PCR) analyses for expression of secreted PCSK6 isoform in a separate cohort of n=233 carotid plaques (E and F). Micorarray data expressed as log2 value and qRT-PCR data as fold change compared with control. Data in A, C, and D show mean with SD, whereas median with interquartile range is depicted in E and F. ER indicates endoplasmatic reticulum.

    Article Snippet: From the list of highly upregulated genes obtained by microarray comparisons between symptomatic and asymptomatic carotid lesions, candidates with relatively restricted tissue distribution in humans were chosen for further analysis (based on the Human Protein Atlas).

    Techniques: Microarray, Expressing, Comparison, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Control