microamp 96 well full plate cover  (Thermo Fisher)


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    Name:
    MicroAmp 96 Well Full Plate Cover
    Description:
    The Applied Biosystems MicroAmp 96 Well Full Plate Cover enables you to seal up to 96 wells in MicroAmp Tray Retainer Set or Reaction Plates to prevent evaporation during thermal cycling of precious samples The durable design will allow for continuous use with high quality results • Convenient cost saving alternative to MicroAmp Caps • Patented one piece design for convenience • Guaranteed reusable up to five times Note See user s manual or package insert for limited label license and trademark information For Research Use Only Not for use in diagnostics procedures
    Catalog Number:
    n8010550
    Price:
    None
    Applications:
    PCR|PCR & Real-Time PCR|PCR Tubes, Plates & Accessories
    Category:
    Lab Supplies Plastics Glassware
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    Structured Review

    Thermo Fisher microamp 96 well full plate cover
    The Applied Biosystems MicroAmp 96 Well Full Plate Cover enables you to seal up to 96 wells in MicroAmp Tray Retainer Set or Reaction Plates to prevent evaporation during thermal cycling of precious samples The durable design will allow for continuous use with high quality results • Convenient cost saving alternative to MicroAmp Caps • Patented one piece design for convenience • Guaranteed reusable up to five times Note See user s manual or package insert for limited label license and trademark information For Research Use Only Not for use in diagnostics procedures
    https://www.bioz.com/result/microamp 96 well full plate cover/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    microamp 96 well full plate cover - by Bioz Stars, 2020-04
    99/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Insight into the Salivary Transcriptome and Proteome of Dipetalogaster maxima
    Article Snippet: The reaction was carried out in MicroAmp 96-well PCR plates (Applied Biosystems, Inc., Fullerton, CA, USA) using FastStart PCR Master (Roche Molecular Biochemicals, Indianapolis, IN, USA) on a GeneAmp® PCR system 9700 (Perkin Elmer Corp.). .. The PCR conditions were: 1 hold of 75°C for 3 min, 1 hold of 94°C for 4 min, 33 cycles of 94°C for 1 min, 49°C for 1 min, and 72°C for 1 min 20 s. The amplified products were analyzed on a 1.2% agarose/EtBr gel. cDNA library clones (2880 clones) were PCR amplified, and those showing a single band were selected for sequencing.

    Article Title: An Insight Into the Sialotranscriptome of Triatoma rubida (Hemiptera: Heteroptera)
    Article Snippet: The reaction was carried out in MicroAmp 96-well PCR plates (Applied Biosystems, Inc., Fullerton, CA) using FastStart PCR Master (Roche Molecular Biochemicals, Indianapolis, IN) on a GeneAmp PCR system 9700 (Perkin Elmer Corp.). .. The PCR conditions were: one hold of 75°C for 3 min, one hold of 94°C for 4 min, 30 cycles of 94°C for 1 min, 49°C for 1 min, and 72°C for 1 min 20 s. The amplified products were analyzed on a 1.2% agarose/EtBr gel. cDNA library clones (1,880 clones) were PCR amplified, and those showing a single band were selected for sequencing.

    Negative Control:

    Article Title: Mechanisms of Heme Utilization by Francisella tularensis
    Article Snippet: A negative control was prepared by excluding the enzyme from the cDNA synthesis (control 1). .. The reactions were performed in MicroAmp 96-well plate (Applied Biosystems) capped with MicroAmp optical adhesive seal.

    Article Title: The 58-Kilodalton Major Virulence Factor of Francisella tularensis Is Required for Efficient Utilization of Iron
    Article Snippet: A negative control was prepared by excluding the enzyme from the cDNA synthesis. .. The reactions were performed in a MicroAmp 96-well plate (Applied Biosystems) capped with MicroAmp optical adhesive seal.

    Article Title: Iron Content Differs between Francisella tularensis Subspecies tularensis and Subspecies holarctica Strains and Correlates to Their Susceptibility to H2O2-Induced Killing ▿-Induced Killing ▿ †
    Article Snippet: A negative control was prepared by excluding the enzyme from the cDNA synthesis. .. The reactions were performed in a MicroAmp 96-well plate (Applied Biosystems) capped with MicroAmp optical adhesive seal.

    Amplification:

    Article Title: Patients with Incompetent Valves in Chronic Venous Insufficiency Show Increased Systematic Lipid Peroxidation and Cellular Oxidative Stress Markers
    Article Snippet: .. For this, 5 μ l of each sample was mixed 1/20 with 10 μ l of iQ™ SYBR® Green Supermix (Bio-Rad Laboratories), 1 μ l of forward primer, 1 μ l of reverse primer (reverse primer), and 3 μ l of DNase and RNase-free water in a MicroAmp® 96-well plate (Thermo Fisher Scientific), for a total reaction volume of 20 μ l. Fluorescence detection was performed at the end of each repetition cycle (amplification) and at each step of the dissociation curve. ..

    Article Title: Insight into the Salivary Transcriptome and Proteome of Dipetalogaster maxima
    Article Snippet: The reaction was carried out in MicroAmp 96-well PCR plates (Applied Biosystems, Inc., Fullerton, CA, USA) using FastStart PCR Master (Roche Molecular Biochemicals, Indianapolis, IN, USA) on a GeneAmp® PCR system 9700 (Perkin Elmer Corp.). .. The PCR conditions were: 1 hold of 75°C for 3 min, 1 hold of 94°C for 4 min, 33 cycles of 94°C for 1 min, 49°C for 1 min, and 72°C for 1 min 20 s. The amplified products were analyzed on a 1.2% agarose/EtBr gel. cDNA library clones (2880 clones) were PCR amplified, and those showing a single band were selected for sequencing.

    Article Title: Preparation of Protein Samples for NMR Structure, Function, and Small Molecule Screening Studies
    Article Snippet: .. The PCR plate is covered with a MicroAmp™ 96-Well Full Plate Cover (Applied Biosystems) and transferred to a Veriti® (Applied Biosystems) PCR thermocycler for amplification. ..

    Article Title: Implication of the PI3K/Akt/mTOR Pathway in the Process of Incompetent Valves in Patients with Chronic Venous Insufficiency and the Relationship with Aging
    Article Snippet: .. To this end, 5 μ L of each sample, previously diluted 1/20 in nuclease-free water, was mixed with 10 μ L of iQ™ SYBR® Green Supermix (Bio-Rad Laboratories), 1 μ L forward primer, 1 μ L Μl of reverse primer, and 3 μ L of DNase and RNase-free water in a MicroAmp® 96-well plate (Applied Biosystems-Life Technologies), for a total reaction volume of 20 μ L. Fluorescence detection is performed at the end of each repeat (amplification) cycle and at each step of the dissociation curve. ..

    Article Title: An Insight Into the Sialotranscriptome of Triatoma rubida (Hemiptera: Heteroptera)
    Article Snippet: The reaction was carried out in MicroAmp 96-well PCR plates (Applied Biosystems, Inc., Fullerton, CA) using FastStart PCR Master (Roche Molecular Biochemicals, Indianapolis, IN) on a GeneAmp PCR system 9700 (Perkin Elmer Corp.). .. The PCR conditions were: one hold of 75°C for 3 min, one hold of 94°C for 4 min, 30 cycles of 94°C for 1 min, 49°C for 1 min, and 72°C for 1 min 20 s. The amplified products were analyzed on a 1.2% agarose/EtBr gel. cDNA library clones (1,880 clones) were PCR amplified, and those showing a single band were selected for sequencing.

    Article Title: Implication of the PI3K/Akt/mTOR Pathway in the Process of Incompetent Valves in Patients with Chronic Venous Insufficiency and the Relationship with Aging
    Article Snippet: .. To this end, 5 μ L of each sample, previously diluted 1/20 in nuclease-free water, was mixed with 10 μ L of iQ™ SYBR® Green Supermix (Bio-Rad Laboratories), 1 μ L forward primer, 1 μ L Μl of reverse primer, and 3 μ L of DNase and RNase-free water in a MicroAmp® 96-well plate (Applied Biosystems-Life Technologies), for a total reaction volume of 20 μ L. Fluorescence detection is performed at the end of each repeat (amplification) cycle and at each step of the dissociation curve. ..

    Fluorescence:

    Article Title: Patients with Incompetent Valves in Chronic Venous Insufficiency Show Increased Systematic Lipid Peroxidation and Cellular Oxidative Stress Markers
    Article Snippet: .. For this, 5 μ l of each sample was mixed 1/20 with 10 μ l of iQ™ SYBR® Green Supermix (Bio-Rad Laboratories), 1 μ l of forward primer, 1 μ l of reverse primer (reverse primer), and 3 μ l of DNase and RNase-free water in a MicroAmp® 96-well plate (Thermo Fisher Scientific), for a total reaction volume of 20 μ l. Fluorescence detection was performed at the end of each repetition cycle (amplification) and at each step of the dissociation curve. ..

    Article Title: Implication of the PI3K/Akt/mTOR Pathway in the Process of Incompetent Valves in Patients with Chronic Venous Insufficiency and the Relationship with Aging
    Article Snippet: .. To this end, 5 μ L of each sample, previously diluted 1/20 in nuclease-free water, was mixed with 10 μ L of iQ™ SYBR® Green Supermix (Bio-Rad Laboratories), 1 μ L forward primer, 1 μ L Μl of reverse primer, and 3 μ L of DNase and RNase-free water in a MicroAmp® 96-well plate (Applied Biosystems-Life Technologies), for a total reaction volume of 20 μ L. Fluorescence detection is performed at the end of each repeat (amplification) cycle and at each step of the dissociation curve. ..

    Article Title: Implication of the PI3K/Akt/mTOR Pathway in the Process of Incompetent Valves in Patients with Chronic Venous Insufficiency and the Relationship with Aging
    Article Snippet: .. To this end, 5 μ L of each sample, previously diluted 1/20 in nuclease-free water, was mixed with 10 μ L of iQ™ SYBR® Green Supermix (Bio-Rad Laboratories), 1 μ L forward primer, 1 μ L Μl of reverse primer, and 3 μ L of DNase and RNase-free water in a MicroAmp® 96-well plate (Applied Biosystems-Life Technologies), for a total reaction volume of 20 μ L. Fluorescence detection is performed at the end of each repeat (amplification) cycle and at each step of the dissociation curve. ..

    Synthesized:

    Article Title: Mechanisms of Heme Utilization by Francisella tularensis
    Article Snippet: The RNA was quantified by Nanodrop (Thermo Fisher Scientific, Wilmington, DE, USA). cDNA was synthesized from 1 μg of the extracted RNA using RNIscript (BioRad) according to the protocol provided by the manufacturer. .. The reactions were performed in MicroAmp 96-well plate (Applied Biosystems) capped with MicroAmp optical adhesive seal.

    Article Title: The 58-Kilodalton Major Virulence Factor of Francisella tularensis Is Required for Efficient Utilization of Iron
    Article Snippet: The RNA was quantified by Nanodrop (Thermo Fisher Scientific, Wilmington, DE). cDNA was synthesized from 1 μg of the extracted RNA using iscript (Bio-Rad) according to the protocol provided by the manufacturer. .. The reactions were performed in a MicroAmp 96-well plate (Applied Biosystems) capped with MicroAmp optical adhesive seal.

    Transferring:

    Article Title: Preparation of Protein Samples for NMR Structure, Function, and Small Molecule Screening Studies
    Article Snippet: An ABI thermocycler compatible 96-well PCR plate is chilled (4°C) on a temperature controlled BioRobot 8000 slot, and the eight-channel pipette head transfers 46 μL of an appropriate PCR reaction mix (dNTPs, high-fidelity thermostable DNA polymerase and buffer, template DNA, and nuclease free H2 O) to each well in a 96-well PCR plate. .. The PCR plate is covered with a MicroAmp™ 96-Well Full Plate Cover (Applied Biosystems) and transferred to a Veriti® (Applied Biosystems) PCR thermocycler for amplification.

    Sequencing:

    Article Title: Mechanisms of Heme Utilization by Francisella tularensis
    Article Snippet: RT-PCR was performed in the ABI Prism 7900HT Sequence Detection System (Applied Biosystems, Foster City, CA, USA) using the SYBR green I PCR kit (Applied Biosystems) as recommended by the manufacturer. .. The reactions were performed in MicroAmp 96-well plate (Applied Biosystems) capped with MicroAmp optical adhesive seal.

    Article Title: The 58-Kilodalton Major Virulence Factor of Francisella tularensis Is Required for Efficient Utilization of Iron
    Article Snippet: RT-PCR was performed with the ABI Prism 7900HT sequence detection system (Applied Biosystems, Foster City, CA) using the SYBR green I PCR kit (Applied Biosystems) as recommended by the manufacturer. .. The reactions were performed in a MicroAmp 96-well plate (Applied Biosystems) capped with MicroAmp optical adhesive seal.

    Article Title: Insight into the Salivary Transcriptome and Proteome of Dipetalogaster maxima
    Article Snippet: Paragraph title: Sequencing of the Dm cDNA Library ... The reaction was carried out in MicroAmp 96-well PCR plates (Applied Biosystems, Inc., Fullerton, CA, USA) using FastStart PCR Master (Roche Molecular Biochemicals, Indianapolis, IN, USA) on a GeneAmp® PCR system 9700 (Perkin Elmer Corp.).

    Article Title: An Insight Into the Sialotranscriptome of Triatoma rubida (Hemiptera: Heteroptera)
    Article Snippet: Paragraph title: Sequencing of the cDNA Library ... The reaction was carried out in MicroAmp 96-well PCR plates (Applied Biosystems, Inc., Fullerton, CA) using FastStart PCR Master (Roche Molecular Biochemicals, Indianapolis, IN) on a GeneAmp PCR system 9700 (Perkin Elmer Corp.).

    Article Title: Iron Content Differs between Francisella tularensis Subspecies tularensis and Subspecies holarctica Strains and Correlates to Their Susceptibility to H2O2-Induced Killing ▿-Induced Killing ▿ †
    Article Snippet: RT-PCR was performed in an ABI Prism 7900HT sequence detection system (Applied Biosystems, Foster City, CA) using a SYBR green I PCR kit (Applied Biosystems), as recommended by the manufacturer. .. The reactions were performed in a MicroAmp 96-well plate (Applied Biosystems) capped with MicroAmp optical adhesive seal.

    Article Title: An Insight into the Sialotranscriptome of Triatoma matogrossensis, a Kissing Bug Associated with Fogo Selvagem in South America
    Article Snippet: Paragraph title: Sequencing of the T. matogrossensis cDNA library. ... The reaction was carried out in MicroAmp 96-well PCR plates (Applied Biosystems, Fullerton, CA) using FastStart PCR Master (Roche Molecular Biochemicals, Indianapolis, IN) on a GeneAmp PCR system 9700 (Perkin Elmer Corp.).

    Quantitative RT-PCR:

    Article Title: Patients with Incompetent Valves in Chronic Venous Insufficiency Show Increased Systematic Lipid Peroxidation and Cellular Oxidative Stress Markers
    Article Snippet: Paragraph title: 2.2. RT-qPCR ... For this, 5 μ l of each sample was mixed 1/20 with 10 μ l of iQ™ SYBR® Green Supermix (Bio-Rad Laboratories), 1 μ l of forward primer, 1 μ l of reverse primer (reverse primer), and 3 μ l of DNase and RNase-free water in a MicroAmp® 96-well plate (Thermo Fisher Scientific), for a total reaction volume of 20 μ l. Fluorescence detection was performed at the end of each repetition cycle (amplification) and at each step of the dissociation curve.

    Concentration Assay:

    Article Title: Cellular and Biophysical Pipeline for the Screening of Peroxisome Proliferator-Activated Receptor Beta/Delta Agonists: Avoiding False Positives
    Article Snippet: Qualitative TSA used 10 μ M of hPPARß/ δ LBD, 5x SYPRO® Orange (Sigma Aldrich) in buffer containing 20 mM HEPES pH 7.5, 200 mM NaCl, and 5% glycerol, and 30 μ M (~0.01 μ g/mL) of commercial agonists (1 protein : 3 ligand) or 140 μ g/mL of extracts from the tested library were added in a Microamp 96-well plate (Applied Biosystems). .. Quantitative TSA was performed in same conditions, varying ligand/extract concentrations (ligand concentrations were 0.3, 0.5, 1, 3, 5, 10, 30, and 50 μ M; extract concentration were 5, 10, 30, 50, 100, 300, and 500 μ g/mL).

    Article Title: Preparation of Protein Samples for NMR Structure, Function, and Small Molecule Screening Studies
    Article Snippet: Briefly, PLIMS-generated 96-well primer plates are provided by the vendor (Eurofin MWG Operon) at 50 μM concentration. .. The PCR plate is covered with a MicroAmp™ 96-Well Full Plate Cover (Applied Biosystems) and transferred to a Veriti® (Applied Biosystems) PCR thermocycler for amplification.

    Article Title: Cellular and Biophysical Pipeline for the Screening of Peroxisome Proliferator-Activated Receptor Beta/Delta Agonists: Avoiding False Positives
    Article Snippet: Qualitative TSA used 10 μ M of hPPARß/δ LBD, 5x SYPRO® Orange (Sigma Aldrich) in buffer containing 20 mM HEPES pH 7.5, 200 mM NaCl, and 5% glycerol, and 30 μ M (~0.01 μ g/mL) of commercial agonists (1 protein : 3 ligand) or 140 μ g/mL of extracts from the tested library were added in a Microamp 96-well plate (Applied Biosystems). .. Quantitative TSA was performed in same conditions, varying ligand/extract concentrations (ligand concentrations were 0.3, 0.5, 1, 3, 5, 10, 30, and 50 μ M; extract concentration were 5, 10, 30, 50, 100, 300, and 500 μ g/mL).

    SYBR Green Assay:

    Article Title: Mechanisms of Heme Utilization by Francisella tularensis
    Article Snippet: Each reaction contained 12.5 μl of the SYBR green mix, 250 nM of forward and reverse primers, 5 μl of a cDNA and the total volume was adjusted with water to 25 μl. .. The reactions were performed in MicroAmp 96-well plate (Applied Biosystems) capped with MicroAmp optical adhesive seal.

    Article Title: The 58-Kilodalton Major Virulence Factor of Francisella tularensis Is Required for Efficient Utilization of Iron
    Article Snippet: Each reaction mixture contained 12.5 μl of the SYBR green mix, 250 nM of forward and reverse primers, and 5 μl of a cDNA, and the total volume was adjusted with water to 25 μl. .. The reactions were performed in a MicroAmp 96-well plate (Applied Biosystems) capped with MicroAmp optical adhesive seal.

    Article Title: Patients with Incompetent Valves in Chronic Venous Insufficiency Show Increased Systematic Lipid Peroxidation and Cellular Oxidative Stress Markers
    Article Snippet: .. For this, 5 μ l of each sample was mixed 1/20 with 10 μ l of iQ™ SYBR® Green Supermix (Bio-Rad Laboratories), 1 μ l of forward primer, 1 μ l of reverse primer (reverse primer), and 3 μ l of DNase and RNase-free water in a MicroAmp® 96-well plate (Thermo Fisher Scientific), for a total reaction volume of 20 μ l. Fluorescence detection was performed at the end of each repetition cycle (amplification) and at each step of the dissociation curve. ..

    Article Title: Implication of the PI3K/Akt/mTOR Pathway in the Process of Incompetent Valves in Patients with Chronic Venous Insufficiency and the Relationship with Aging
    Article Snippet: .. To this end, 5 μ L of each sample, previously diluted 1/20 in nuclease-free water, was mixed with 10 μ L of iQ™ SYBR® Green Supermix (Bio-Rad Laboratories), 1 μ L forward primer, 1 μ L Μl of reverse primer, and 3 μ L of DNase and RNase-free water in a MicroAmp® 96-well plate (Applied Biosystems-Life Technologies), for a total reaction volume of 20 μ L. Fluorescence detection is performed at the end of each repeat (amplification) cycle and at each step of the dissociation curve. ..

    Article Title: Iron Content Differs between Francisella tularensis Subspecies tularensis and Subspecies holarctica Strains and Correlates to Their Susceptibility to H2O2-Induced Killing ▿-Induced Killing ▿ †
    Article Snippet: Each reaction mixture contained 12.5 μl of the SYBR green mix, 250 nM forward and reverse primers, and 5 μl of cDNA, and the total volume was adjusted to 25 μl with water. .. The reactions were performed in a MicroAmp 96-well plate (Applied Biosystems) capped with MicroAmp optical adhesive seal.

    Article Title: Implication of the PI3K/Akt/mTOR Pathway in the Process of Incompetent Valves in Patients with Chronic Venous Insufficiency and the Relationship with Aging
    Article Snippet: .. To this end, 5 μ L of each sample, previously diluted 1/20 in nuclease-free water, was mixed with 10 μ L of iQ™ SYBR® Green Supermix (Bio-Rad Laboratories), 1 μ L forward primer, 1 μ L Μl of reverse primer, and 3 μ L of DNase and RNase-free water in a MicroAmp® 96-well plate (Applied Biosystems-Life Technologies), for a total reaction volume of 20 μ L. Fluorescence detection is performed at the end of each repeat (amplification) cycle and at each step of the dissociation curve. ..

    Polymerase Chain Reaction:

    Article Title: Mechanisms of Heme Utilization by Francisella tularensis
    Article Snippet: RT-PCR was performed in the ABI Prism 7900HT Sequence Detection System (Applied Biosystems, Foster City, CA, USA) using the SYBR green I PCR kit (Applied Biosystems) as recommended by the manufacturer. .. The reactions were performed in MicroAmp 96-well plate (Applied Biosystems) capped with MicroAmp optical adhesive seal.

    Article Title: The 58-Kilodalton Major Virulence Factor of Francisella tularensis Is Required for Efficient Utilization of Iron
    Article Snippet: RT-PCR was performed with the ABI Prism 7900HT sequence detection system (Applied Biosystems, Foster City, CA) using the SYBR green I PCR kit (Applied Biosystems) as recommended by the manufacturer. .. The reactions were performed in a MicroAmp 96-well plate (Applied Biosystems) capped with MicroAmp optical adhesive seal.

    Article Title: Cellular and Biophysical Pipeline for the Screening of Peroxisome Proliferator-Activated Receptor Beta/Delta Agonists: Avoiding False Positives
    Article Snippet: Qualitative TSA used 10 μ M of hPPARß/ δ LBD, 5x SYPRO® Orange (Sigma Aldrich) in buffer containing 20 mM HEPES pH 7.5, 200 mM NaCl, and 5% glycerol, and 30 μ M (~0.01 μ g/mL) of commercial agonists (1 protein : 3 ligand) or 140 μ g/mL of extracts from the tested library were added in a Microamp 96-well plate (Applied Biosystems). .. The experiment was performed at 7500 PCR Real Time System (Applied Biosystems), and measurements were taken from 9°C to 89°C, with a gradient of 1°C per minute, totalizing 80 measurements [ ].

    Article Title: Insight into the Salivary Transcriptome and Proteome of Dipetalogaster maxima
    Article Snippet: .. The reaction was carried out in MicroAmp 96-well PCR plates (Applied Biosystems, Inc., Fullerton, CA, USA) using FastStart PCR Master (Roche Molecular Biochemicals, Indianapolis, IN, USA) on a GeneAmp® PCR system 9700 (Perkin Elmer Corp.). .. The PCR conditions were: 1 hold of 75°C for 3 min, 1 hold of 94°C for 4 min, 33 cycles of 94°C for 1 min, 49°C for 1 min, and 72°C for 1 min 20 s. The amplified products were analyzed on a 1.2% agarose/EtBr gel. cDNA library clones (2880 clones) were PCR amplified, and those showing a single band were selected for sequencing.

    Article Title: Preparation of Protein Samples for NMR Structure, Function, and Small Molecule Screening Studies
    Article Snippet: .. The PCR plate is covered with a MicroAmp™ 96-Well Full Plate Cover (Applied Biosystems) and transferred to a Veriti® (Applied Biosystems) PCR thermocycler for amplification. ..

    Article Title: An Insight Into the Sialotranscriptome of Triatoma rubida (Hemiptera: Heteroptera)
    Article Snippet: .. The reaction was carried out in MicroAmp 96-well PCR plates (Applied Biosystems, Inc., Fullerton, CA) using FastStart PCR Master (Roche Molecular Biochemicals, Indianapolis, IN) on a GeneAmp PCR system 9700 (Perkin Elmer Corp.). .. The PCR conditions were: one hold of 75°C for 3 min, one hold of 94°C for 4 min, 30 cycles of 94°C for 1 min, 49°C for 1 min, and 72°C for 1 min 20 s. The amplified products were analyzed on a 1.2% agarose/EtBr gel. cDNA library clones (1,880 clones) were PCR amplified, and those showing a single band were selected for sequencing.

    Article Title: Iron Content Differs between Francisella tularensis Subspecies tularensis and Subspecies holarctica Strains and Correlates to Their Susceptibility to H2O2-Induced Killing ▿-Induced Killing ▿ †
    Article Snippet: RT-PCR was performed in an ABI Prism 7900HT sequence detection system (Applied Biosystems, Foster City, CA) using a SYBR green I PCR kit (Applied Biosystems), as recommended by the manufacturer. .. The reactions were performed in a MicroAmp 96-well plate (Applied Biosystems) capped with MicroAmp optical adhesive seal.

    Article Title: Cellular and Biophysical Pipeline for the Screening of Peroxisome Proliferator-Activated Receptor Beta/Delta Agonists: Avoiding False Positives
    Article Snippet: Qualitative TSA used 10 μ M of hPPARß/δ LBD, 5x SYPRO® Orange (Sigma Aldrich) in buffer containing 20 mM HEPES pH 7.5, 200 mM NaCl, and 5% glycerol, and 30 μ M (~0.01 μ g/mL) of commercial agonists (1 protein : 3 ligand) or 140 μ g/mL of extracts from the tested library were added in a Microamp 96-well plate (Applied Biosystems). .. The experiment was performed at 7500 PCR Real Time System (Applied Biosystems), and measurements were taken from 9°C to 89°C, with a gradient of 1°C per minute, totalizing 80 measurements [ ].

    Article Title: An Insight into the Sialotranscriptome of Triatoma matogrossensis, a Kissing Bug Associated with Fogo Selvagem in South America
    Article Snippet: .. The reaction was carried out in MicroAmp 96-well PCR plates (Applied Biosystems, Fullerton, CA) using FastStart PCR Master (Roche Molecular Biochemicals, Indianapolis, IN) on a GeneAmp PCR system 9700 (Perkin Elmer Corp.). ..

    Plasmid Preparation:

    Article Title: Insight into the Salivary Transcriptome and Proteome of Dipetalogaster maxima
    Article Snippet: The primers were sequences from the λ TriplEx2 vector and named PT2F1 (5′-AAG TACTCTAGCAATTGTGAGC-3′) and PT2R1 (5′-CTCTTCGCTATTACGCCAGCTG-3′), positioned at the 5′ and 3′ end of the cDNA insert, respectively. .. The reaction was carried out in MicroAmp 96-well PCR plates (Applied Biosystems, Inc., Fullerton, CA, USA) using FastStart PCR Master (Roche Molecular Biochemicals, Indianapolis, IN, USA) on a GeneAmp® PCR system 9700 (Perkin Elmer Corp.).

    Article Title: An Insight Into the Sialotranscriptome of Triatoma rubida (Hemiptera: Heteroptera)
    Article Snippet: The primers were sequences from the λ TriplEx2 vector and named PT2F1 (5′-AAG TACTCTAGCAATTGTGAGC-3′) and PT2R1 (5′-CTCTTCGCTATTACGCCAGCTG-3′), positioned at the 5′ and 3′ end of the cDNA insert, respectively. .. The reaction was carried out in MicroAmp 96-well PCR plates (Applied Biosystems, Inc., Fullerton, CA) using FastStart PCR Master (Roche Molecular Biochemicals, Indianapolis, IN) on a GeneAmp PCR system 9700 (Perkin Elmer Corp.).

    Article Title: An Insight into the Sialotranscriptome of Triatoma matogrossensis, a Kissing Bug Associated with Fogo Selvagem in South America
    Article Snippet: The primers were sequences from the λ TriplEx2 vector and named PT2F1 (5′-AAG TACTCTAGCAATTGTGAGC-3′) and PT2R1 (5′-CTCTTCGCTATTACGCCAGCTG-3′), positioned at the 5′ and 3′ end of the cDNA insert, respectively. .. The reaction was carried out in MicroAmp 96-well PCR plates (Applied Biosystems, Fullerton, CA) using FastStart PCR Master (Roche Molecular Biochemicals, Indianapolis, IN) on a GeneAmp PCR system 9700 (Perkin Elmer Corp.).

    Thermal Shift Assay:

    Article Title: Cellular and Biophysical Pipeline for the Screening of Peroxisome Proliferator-Activated Receptor Beta/Delta Agonists: Avoiding False Positives
    Article Snippet: Paragraph title: 2.7. Thermal Shift Assay (TSA) ... Qualitative TSA used 10 μ M of hPPARß/ δ LBD, 5x SYPRO® Orange (Sigma Aldrich) in buffer containing 20 mM HEPES pH 7.5, 200 mM NaCl, and 5% glycerol, and 30 μ M (~0.01 μ g/mL) of commercial agonists (1 protein : 3 ligand) or 140 μ g/mL of extracts from the tested library were added in a Microamp 96-well plate (Applied Biosystems).

    cDNA Library Assay:

    Article Title: Insight into the Salivary Transcriptome and Proteome of Dipetalogaster maxima
    Article Snippet: Paragraph title: Sequencing of the Dm cDNA Library ... The reaction was carried out in MicroAmp 96-well PCR plates (Applied Biosystems, Inc., Fullerton, CA, USA) using FastStart PCR Master (Roche Molecular Biochemicals, Indianapolis, IN, USA) on a GeneAmp® PCR system 9700 (Perkin Elmer Corp.).

    Article Title: An Insight Into the Sialotranscriptome of Triatoma rubida (Hemiptera: Heteroptera)
    Article Snippet: Paragraph title: Sequencing of the cDNA Library ... The reaction was carried out in MicroAmp 96-well PCR plates (Applied Biosystems, Inc., Fullerton, CA) using FastStart PCR Master (Roche Molecular Biochemicals, Indianapolis, IN) on a GeneAmp PCR system 9700 (Perkin Elmer Corp.).

    Article Title: An Insight into the Sialotranscriptome of Triatoma matogrossensis, a Kissing Bug Associated with Fogo Selvagem in South America
    Article Snippet: Paragraph title: Sequencing of the T. matogrossensis cDNA library. ... The reaction was carried out in MicroAmp 96-well PCR plates (Applied Biosystems, Fullerton, CA) using FastStart PCR Master (Roche Molecular Biochemicals, Indianapolis, IN) on a GeneAmp PCR system 9700 (Perkin Elmer Corp.).

    Labeling:

    Article Title: Insight into the Salivary Transcriptome and Proteome of Dipetalogaster maxima
    Article Snippet: The reaction was carried out in MicroAmp 96-well PCR plates (Applied Biosystems, Inc., Fullerton, CA, USA) using FastStart PCR Master (Roche Molecular Biochemicals, Indianapolis, IN, USA) on a GeneAmp® PCR system 9700 (Perkin Elmer Corp.). .. The PCR products were used as a template for a cycle-sequencing reaction using a DTCS labeling kit (Beckman Coulter, Fullerton, CA, USA).

    Article Title: An Insight Into the Sialotranscriptome of Triatoma rubida (Hemiptera: Heteroptera)
    Article Snippet: The reaction was carried out in MicroAmp 96-well PCR plates (Applied Biosystems, Inc., Fullerton, CA) using FastStart PCR Master (Roche Molecular Biochemicals, Indianapolis, IN) on a GeneAmp PCR system 9700 (Perkin Elmer Corp.). .. The PCR products were used as a template for a cycle-sequencing reaction using a DTCS labeling kit (Beckman Coulter, Fullerton, CA).

    Article Title: An Insight into the Sialotranscriptome of Triatoma matogrossensis, a Kissing Bug Associated with Fogo Selvagem in South America
    Article Snippet: The reaction was carried out in MicroAmp 96-well PCR plates (Applied Biosystems, Fullerton, CA) using FastStart PCR Master (Roche Molecular Biochemicals, Indianapolis, IN) on a GeneAmp PCR system 9700 (Perkin Elmer Corp.). .. The PCR products were used as a template for a cycle-sequencing reaction using the DTCS labeling kit (Beckman Coulter, Inc., Fullerton, CA).

    Expressing:

    Article Title: Mechanisms of Heme Utilization by Francisella tularensis
    Article Snippet: Paragraph title: Real-time PCR analysis of gene expression ... The reactions were performed in MicroAmp 96-well plate (Applied Biosystems) capped with MicroAmp optical adhesive seal.

    Article Title: The 58-Kilodalton Major Virulence Factor of Francisella tularensis Is Required for Efficient Utilization of Iron
    Article Snippet: Paragraph title: Analysis of gene expression. ... The reactions were performed in a MicroAmp 96-well plate (Applied Biosystems) capped with MicroAmp optical adhesive seal.

    Article Title: Patients with Incompetent Valves in Chronic Venous Insufficiency Show Increased Systematic Lipid Peroxidation and Cellular Oxidative Stress Markers
    Article Snippet: Gene expression was normalized using GAPDH as reference gene. .. For this, 5 μ l of each sample was mixed 1/20 with 10 μ l of iQ™ SYBR® Green Supermix (Bio-Rad Laboratories), 1 μ l of forward primer, 1 μ l of reverse primer (reverse primer), and 3 μ l of DNase and RNase-free water in a MicroAmp® 96-well plate (Thermo Fisher Scientific), for a total reaction volume of 20 μ l. Fluorescence detection was performed at the end of each repetition cycle (amplification) and at each step of the dissociation curve.

    Article Title: Preparation of Protein Samples for NMR Structure, Function, and Small Molecule Screening Studies
    Article Snippet: Paragraph title: High-throughput construction of expression plasmids ... The PCR plate is covered with a MicroAmp™ 96-Well Full Plate Cover (Applied Biosystems) and transferred to a Veriti® (Applied Biosystems) PCR thermocycler for amplification.

    Article Title: Implication of the PI3K/Akt/mTOR Pathway in the Process of Incompetent Valves in Patients with Chronic Venous Insufficiency and the Relationship with Aging
    Article Snippet: Paragraph title: 2.5. Genetic Expression ... To this end, 5 μ L of each sample, previously diluted 1/20 in nuclease-free water, was mixed with 10 μ L of iQ™ SYBR® Green Supermix (Bio-Rad Laboratories), 1 μ L forward primer, 1 μ L Μl of reverse primer, and 3 μ L of DNase and RNase-free water in a MicroAmp® 96-well plate (Applied Biosystems-Life Technologies), for a total reaction volume of 20 μ L. Fluorescence detection is performed at the end of each repeat (amplification) cycle and at each step of the dissociation curve.

    Article Title: Iron Content Differs between Francisella tularensis Subspecies tularensis and Subspecies holarctica Strains and Correlates to Their Susceptibility to H2O2-Induced Killing ▿-Induced Killing ▿ †
    Article Snippet: Paragraph title: Analysis of gene expression. ... The reactions were performed in a MicroAmp 96-well plate (Applied Biosystems) capped with MicroAmp optical adhesive seal.

    High Throughput Screening Assay:

    Article Title: Preparation of Protein Samples for NMR Structure, Function, and Small Molecule Screening Studies
    Article Snippet: Paragraph title: High-throughput construction of expression plasmids ... The PCR plate is covered with a MicroAmp™ 96-Well Full Plate Cover (Applied Biosystems) and transferred to a Veriti® (Applied Biosystems) PCR thermocycler for amplification.

    Real-time Polymerase Chain Reaction:

    Article Title: Mechanisms of Heme Utilization by Francisella tularensis
    Article Snippet: Paragraph title: Real-time PCR analysis of gene expression ... The reactions were performed in MicroAmp 96-well plate (Applied Biosystems) capped with MicroAmp optical adhesive seal.

    Article Title: Patients with Incompetent Valves in Chronic Venous Insufficiency Show Increased Systematic Lipid Peroxidation and Cellular Oxidative Stress Markers
    Article Snippet: The qPCR was performed in a StepOnePlus™ System (Thermo Fisher Scientific), and the relative standard curve method was used. .. For this, 5 μ l of each sample was mixed 1/20 with 10 μ l of iQ™ SYBR® Green Supermix (Bio-Rad Laboratories), 1 μ l of forward primer, 1 μ l of reverse primer (reverse primer), and 3 μ l of DNase and RNase-free water in a MicroAmp® 96-well plate (Thermo Fisher Scientific), for a total reaction volume of 20 μ l. Fluorescence detection was performed at the end of each repetition cycle (amplification) and at each step of the dissociation curve.

    Article Title: Implication of the PI3K/Akt/mTOR Pathway in the Process of Incompetent Valves in Patients with Chronic Venous Insufficiency and the Relationship with Aging
    Article Snippet: The qPCR was performed on a StepOnePlus™ System (Applied Biosystems-Life Technologies), using the relative standard curve method. .. To this end, 5 μ L of each sample, previously diluted 1/20 in nuclease-free water, was mixed with 10 μ L of iQ™ SYBR® Green Supermix (Bio-Rad Laboratories), 1 μ L forward primer, 1 μ L Μl of reverse primer, and 3 μ L of DNase and RNase-free water in a MicroAmp® 96-well plate (Applied Biosystems-Life Technologies), for a total reaction volume of 20 μ L. Fluorescence detection is performed at the end of each repeat (amplification) cycle and at each step of the dissociation curve.

    Article Title: Implication of the PI3K/Akt/mTOR Pathway in the Process of Incompetent Valves in Patients with Chronic Venous Insufficiency and the Relationship with Aging
    Article Snippet: The qPCR was performed on a StepOnePlus™ System (Applied Biosystems-Life Technologies), using the relative standard curve method. .. To this end, 5 μ L of each sample, previously diluted 1/20 in nuclease-free water, was mixed with 10 μ L of iQ™ SYBR® Green Supermix (Bio-Rad Laboratories), 1 μ L forward primer, 1 μ L Μl of reverse primer, and 3 μ L of DNase and RNase-free water in a MicroAmp® 96-well plate (Applied Biosystems-Life Technologies), for a total reaction volume of 20 μ L. Fluorescence detection is performed at the end of each repeat (amplification) cycle and at each step of the dissociation curve.

    Recombinant:

    Article Title: Insight into the Salivary Transcriptome and Proteome of Dipetalogaster maxima
    Article Snippet: Recombinant (white) plaques were randomly picked up and transferred to 96-well MicroTest ™ U-bottom plates (BD BioScience, San José, CA, USA) containing 75 μl of H2 O per well. .. The reaction was carried out in MicroAmp 96-well PCR plates (Applied Biosystems, Inc., Fullerton, CA, USA) using FastStart PCR Master (Roche Molecular Biochemicals, Indianapolis, IN, USA) on a GeneAmp® PCR system 9700 (Perkin Elmer Corp.).

    Article Title: An Insight Into the Sialotranscriptome of Triatoma rubida (Hemiptera: Heteroptera)
    Article Snippet: Recombinant (white) plaques were randomly picked up and transferred to 96-well MicroTest U-bottom plates (BD BioScience, San José, CA) containing 75 μ l of H2 O per well. .. The reaction was carried out in MicroAmp 96-well PCR plates (Applied Biosystems, Inc., Fullerton, CA) using FastStart PCR Master (Roche Molecular Biochemicals, Indianapolis, IN) on a GeneAmp PCR system 9700 (Perkin Elmer Corp.).

    Article Title: An Insight into the Sialotranscriptome of Triatoma matogrossensis, a Kissing Bug Associated with Fogo Selvagem in South America
    Article Snippet: Recombinant (white) plaques were randomly picked and transferred to 96-well MICROTEST U-bottom plates (BD BioSciences, San José, CA) containing 75 μL of H2 O per well. .. The reaction was carried out in MicroAmp 96-well PCR plates (Applied Biosystems, Fullerton, CA) using FastStart PCR Master (Roche Molecular Biochemicals, Indianapolis, IN) on a GeneAmp PCR system 9700 (Perkin Elmer Corp.).

    Derivative Assay:

    Article Title: Preparation of Protein Samples for NMR Structure, Function, and Small Molecule Screening Studies
    Article Snippet: The PCR plate is covered with a MicroAmp™ 96-Well Full Plate Cover (Applied Biosystems) and transferred to a Veriti® (Applied Biosystems) PCR thermocycler for amplification. .. An annealing temperature step increase after 10 rounds of amplification (57-60 °C) is included for the final 25 cycles to adjust for the increased stability derived from the added recombination sites base pairs ( ).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Mechanisms of Heme Utilization by Francisella tularensis
    Article Snippet: RT-PCR was performed in the ABI Prism 7900HT Sequence Detection System (Applied Biosystems, Foster City, CA, USA) using the SYBR green I PCR kit (Applied Biosystems) as recommended by the manufacturer. .. The reactions were performed in MicroAmp 96-well plate (Applied Biosystems) capped with MicroAmp optical adhesive seal.

    Article Title: The 58-Kilodalton Major Virulence Factor of Francisella tularensis Is Required for Efficient Utilization of Iron
    Article Snippet: RT-PCR was performed with the ABI Prism 7900HT sequence detection system (Applied Biosystems, Foster City, CA) using the SYBR green I PCR kit (Applied Biosystems) as recommended by the manufacturer. .. The reactions were performed in a MicroAmp 96-well plate (Applied Biosystems) capped with MicroAmp optical adhesive seal.

    Article Title: Iron Content Differs between Francisella tularensis Subspecies tularensis and Subspecies holarctica Strains and Correlates to Their Susceptibility to H2O2-Induced Killing ▿-Induced Killing ▿ †
    Article Snippet: RT-PCR was performed in an ABI Prism 7900HT sequence detection system (Applied Biosystems, Foster City, CA) using a SYBR green I PCR kit (Applied Biosystems), as recommended by the manufacturer. .. The reactions were performed in a MicroAmp 96-well plate (Applied Biosystems) capped with MicroAmp optical adhesive seal.

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  • 99
    Thermo Fisher microamp 96 well plate
    Microamp 96 Well Plate, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/microamp 96 well plate/product/Thermo Fisher
    Average 99 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    microamp 96 well plate - by Bioz Stars, 2020-04
    99/100 stars
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