micro bio spin 6 column  (Bio-Rad)

 
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    Name:
    Micro Bio Spin 6 Columns
    Description:
    Pkg of 25 prepacked spin columns with Bio Gel P 6 in Tris buffer sample volume 10 75 μl 6 000 MW limit includes 50 collection tubes education use only
    Catalog Number:
    7326221EDU
    Price:
    None
    Category:
    Protein Expression and Purification Series
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    Structured Review

    Bio-Rad micro bio spin 6 column
    Micro Bio Spin 6 Columns
    Pkg of 25 prepacked spin columns with Bio Gel P 6 in Tris buffer sample volume 10 75 μl 6 000 MW limit includes 50 collection tubes education use only
    https://www.bioz.com/result/micro bio spin 6 column/product/Bio-Rad
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    micro bio spin 6 column - by Bioz Stars, 2020-04
    99/100 stars

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    Related Articles

    Filtration:

    Article Title: Dynamic active site protection by the M. tuberculosis protein tyrosine phosphatase PtpB lid domain
    Article Snippet: The labeled protein was separated from free dyes using a Superdex 75 gel filtration column (HR-10/30, GE Healthcare) in 20 mM tris (hydroxymethyl)aminomethane (adjusted to pH 7.5 using HCl, abbreviated Tris-HCl) and 100 mM NaCl. .. To remove PO4 from the sample, the protein was separated four times on Micro Bio-Spin™ 6 columns (BioRad) equilibrated in 20 mM Tris-HCl pH 7.5, 100 mM NaCl.

    Article Title: Bacteriophage Xp10 anti-termination factor p7 induces forward translocation by host RNA polymerase
    Article Snippet: .. Electrophoretic mobility shift assay DNA substrates were 5′ end-labelled with with γ[32 P]ATP and PNK, and unincorporated γ[32 P]ATP was removed by gel filtration on Micro-BioSpin 6 columns (BioRad). .. Radiolabelled DNA were mixed with single strand binding protein (SSB) (Sigma-Aldrich), p7 or the storage buffer, incubated for 10 min at 30°C and resolved by 6% native PAGE (0.5× TBE) at room temperature.

    Article Title: Direct observation of TALE protein dynamics reveals a two-state search mechanism
    Article Snippet: When necessary, the protein was further purified using 16/600 200 pg gel filtration column (GE Healthcare) using 50 mM phosphate buffer pH 7–8.4 (depending on the predicted protein isoelectric point), 500 mM NaCl. .. The labelled proteins were diluted with 400 μl fluorescence anisotropy buffer (20 μM Tris-HCl, pH 7.5, 100 mM Nacl, 0.5 mM EDTA (Fisher)) and purified from unreacted Cy3 by two consecutive passages through Micro Bio-spin 6 columns (Bio-Rad) following the manufacturer's instructions.

    Article Title: Discovery of Dengue Virus NS4B Inhibitors
    Article Snippet: Paragraph title: Micro BioSpin column gel filtration assay. ... Micro BioSpin 6 columns (Bio-Rad) capable of separating free ligands from bound ligands were used to measure the binding of 3 H-labeled compound 14a to purified proteins.

    Incubation:

    Article Title: CheY3 of Borrelia burgdorferi Is the Key Response Regulator Essential for Chemotaxis and Forms a Long-Lived Phosphorylated Intermediate ▿
    Article Snippet: .. Micro Bio-Spin6 columns were obtained from Bio-Rad; all other reagents were obtained from Sigma-Aldrich Chemical Co. rCheA1 and rCheA2 (2 μM) were incubated in 50 mM Tris (pH 8.5)–50 mM KCl,–5 mM MgCl2 –0.3 mM ATP–1 μCi [γ-32 P]ATP (6,000 Ci/mmol)/μl for 30 min. ..

    Article Title: Bacteriophage Xp10 anti-termination factor p7 induces forward translocation by host RNA polymerase
    Article Snippet: Electrophoretic mobility shift assay DNA substrates were 5′ end-labelled with with γ[32 P]ATP and PNK, and unincorporated γ[32 P]ATP was removed by gel filtration on Micro-BioSpin 6 columns (BioRad). .. Radiolabelled DNA were mixed with single strand binding protein (SSB) (Sigma-Aldrich), p7 or the storage buffer, incubated for 10 min at 30°C and resolved by 6% native PAGE (0.5× TBE) at room temperature.

    Article Title: Direct observation of TALE protein dynamics reveals a two-state search mechanism
    Article Snippet: The labelled proteins were diluted with 400 μl fluorescence anisotropy buffer (20 μM Tris-HCl, pH 7.5, 100 mM Nacl, 0.5 mM EDTA (Fisher)) and purified from unreacted Cy3 by two consecutive passages through Micro Bio-spin 6 columns (Bio-Rad) following the manufacturer's instructions. .. Purified TALE–biotin was conjugated to the streptavidin-coated Qdot705 (Invitrogen) by incubation with 10-fold molar excess Qdots for 15 min.

    Article Title: Discovery of Dengue Virus NS4B Inhibitors
    Article Snippet: Micro BioSpin 6 columns (Bio-Rad) capable of separating free ligands from bound ligands were used to measure the binding of 3 H-labeled compound 14a to purified proteins. .. A binding mixture (20 μl) containing 20 mM sodium phosphate (pH 6.5), 0.1% 1-myristoyl-2-hydroxy- sn -glycero-3-[phospho-Rac-(1-glycerol)] (LMPG), 1 mM dithiothreitol (DTT), 150 mM NaCl, 5 to 50 μM NS4B, and 100 to 500 μM 3 H-labeled compound 14a was incubated at room temperature for 10 min, adsorbed to a Micro BioSpin column, and centrifuged for 1 min.

    Activity Assay:

    Article Title: Dynamic active site protection by the M. tuberculosis protein tyrosine phosphatase PtpB lid domain
    Article Snippet: To remove PO4 from the sample, the protein was separated four times on Micro Bio-Spin™ 6 columns (BioRad) equilibrated in 20 mM Tris-HCl pH 7.5, 100 mM NaCl. .. Neither ESI-MS on a quadrupole time-of-flight mass spectrometer nor MALDI-MS were able to detect unlabeled or singly labeled proteins, thus the labeling efficiency was assumed to be 100% for the purposes of enzymatic activity and bulk FRET calculations.

    Infection:

    Article Title: Antisense Transcription in Gammaretroviruses as a Mechanism of Insertional Activation of Host Genes ▿
    Article Snippet: NIH 3T3 cells were either not infected or infected with replication-competent SL3-3 or Akv1-99 (EGFP) MLVs and cultured for 2 weeks. .. Residual nucleotides were removed with Micro Bio-Spin 6 columns (Bio-Rad) before being used for hybridization with buffers and conditions described in reference .

    Mass Spectrometry:

    Article Title: Subunit Interactions within the Carbon-Phosphorus Lyase Complex from Escherichia coli
    Article Snippet: ESI MS measurements were acquired with a ThermoScientific Exactive Plus EMR. .. The protein complexes were buffered exchanged into 50 mM ammonium acetate (pH 6.8) prior to analysis using Bio-Rad Micro Bio-Spin® columns.

    Article Title: Hsp70 Oligomerization Is Mediated by an Interaction between the Interdomain Linker and the Substrate-Binding Domain
    Article Snippet: .. Mass spectrometry Prior to MS analysis, samples were buffer exchanged into 150 mM ammonium acetate, pH 7.4, using Micro Bio-spin 6 columns (Bio-Rad, Digilab Division, Cambridge, MA, USA). .. In the case of the substrate binding experiments, proteins were analyzed in the presence of a 1: 1 molar ratio of the NR peptide; this substrate concentration, lower than in the SEC experiments, was chosen to ensure adequate resolution of the various charge state series, which is critical for unambiguous assignment of substrate-bound states.

    Article Title: Dynamic active site protection by the M. tuberculosis protein tyrosine phosphatase PtpB lid domain
    Article Snippet: To remove PO4 from the sample, the protein was separated four times on Micro Bio-Spin™ 6 columns (BioRad) equilibrated in 20 mM Tris-HCl pH 7.5, 100 mM NaCl. .. Neither ESI-MS on a quadrupole time-of-flight mass spectrometer nor MALDI-MS were able to detect unlabeled or singly labeled proteins, thus the labeling efficiency was assumed to be 100% for the purposes of enzymatic activity and bulk FRET calculations.

    Article Title: Structural disorder and induced folding within two cereal, ABA stress and ripening (ASR) proteins
    Article Snippet: As a control, we first measured ADH to confirm that the MS parameters used were suitable to maintain non-covalent complexes. .. Prior to analysis, all proteins were buffer-exchanged into 250 mM ammonium acetate, pH 8.0 using Micro Bio-SpinTM 6 Columns (Bio-Rad).

    Article Title: Structures of DPAGT1 Explain Glycosylation Disease Mechanisms and Advance TB Antibiotic Design
    Article Snippet: .. Native mass spectrometry For native mass spectrometry analysis purified DPAGT1 protein was diluted to 10 μM protein concentration using 200 mM ammonium acetate supplemented with 0.16% OGNG solution followed by buffer exchanged into 200 mM ammonium acetate, 0.16% OGNG using a micro biospin column (Micro Bio-Spin 6, Bio-Rad). .. Native MS experiments were conducted using a Q Exactive instrument (Thermo Fisher, Germany) with modifications for high-mass transmission optimization.

    Hybridization:

    Article Title: Antisense Transcription in Gammaretroviruses as a Mechanism of Insertional Activation of Host Genes ▿
    Article Snippet: .. Residual nucleotides were removed with Micro Bio-Spin 6 columns (Bio-Rad) before being used for hybridization with buffers and conditions described in reference . ..

    Flow Cytometry:

    Article Title: Structural disorder and induced folding within two cereal, ABA stress and ripening (ASR) proteins
    Article Snippet: Prior to analysis, all proteins were buffer-exchanged into 250 mM ammonium acetate, pH 8.0 using Micro Bio-SpinTM 6 Columns (Bio-Rad). .. The capillary voltage was set to 1.8–2.1 kV, the source temperature was 30 °C, the sampling cone was set to 150 V, the source offset to 150 V, and the trap gas flow to 4 mL/min.

    Northern Blot:

    Article Title: Antisense Transcription in Gammaretroviruses as a Mechanism of Insertional Activation of Host Genes ▿
    Article Snippet: Paragraph title: Northern blotting. ... Residual nucleotides were removed with Micro Bio-Spin 6 columns (Bio-Rad) before being used for hybridization with buffers and conditions described in reference .

    Cell Culture:

    Article Title: Antisense Transcription in Gammaretroviruses as a Mechanism of Insertional Activation of Host Genes ▿
    Article Snippet: NIH 3T3 cells were either not infected or infected with replication-competent SL3-3 or Akv1-99 (EGFP) MLVs and cultured for 2 weeks. .. Residual nucleotides were removed with Micro Bio-Spin 6 columns (Bio-Rad) before being used for hybridization with buffers and conditions described in reference .

    other:

    Article Title: The glyceraldehyde-3-phosphate dehydrogenase GapDH of Corynebacterium diphtheriae is redox-controlled by protein S-mycothiolation under oxidative stress
    Article Snippet: Excess of DTT was removed by desalting with Micro Biospin 6 columns.

    Protein Concentration:

    Article Title: Structures of DPAGT1 Explain Glycosylation Disease Mechanisms and Advance TB Antibiotic Design
    Article Snippet: .. Native mass spectrometry For native mass spectrometry analysis purified DPAGT1 protein was diluted to 10 μM protein concentration using 200 mM ammonium acetate supplemented with 0.16% OGNG solution followed by buffer exchanged into 200 mM ammonium acetate, 0.16% OGNG using a micro biospin column (Micro Bio-Spin 6, Bio-Rad). .. Native MS experiments were conducted using a Q Exactive instrument (Thermo Fisher, Germany) with modifications for high-mass transmission optimization.

    Injection:

    Article Title: Subunit Interactions within the Carbon-Phosphorus Lyase Complex from Escherichia coli
    Article Snippet: The protein complexes were buffered exchanged into 50 mM ammonium acetate (pH 6.8) prior to analysis using Bio-Rad Micro Bio-Spin® columns. .. Important instrument parameters used were: FT resolution was set to 17,500, S-Lens RF = 200, S-Lens = 42 V, Skimmer = 15 V, ion injection time was limited to 200 ms, AGC target set to 1,000,000, and 40 microscans were acquired.

    Binding Assay:

    Article Title: Hsp70 Oligomerization Is Mediated by an Interaction between the Interdomain Linker and the Substrate-Binding Domain
    Article Snippet: Mass spectrometry Prior to MS analysis, samples were buffer exchanged into 150 mM ammonium acetate, pH 7.4, using Micro Bio-spin 6 columns (Bio-Rad, Digilab Division, Cambridge, MA, USA). .. In the case of the substrate binding experiments, proteins were analyzed in the presence of a 1: 1 molar ratio of the NR peptide; this substrate concentration, lower than in the SEC experiments, was chosen to ensure adequate resolution of the various charge state series, which is critical for unambiguous assignment of substrate-bound states.

    Article Title: Bacteriophage Xp10 anti-termination factor p7 induces forward translocation by host RNA polymerase
    Article Snippet: Electrophoretic mobility shift assay DNA substrates were 5′ end-labelled with with γ[32 P]ATP and PNK, and unincorporated γ[32 P]ATP was removed by gel filtration on Micro-BioSpin 6 columns (BioRad). .. Radiolabelled DNA were mixed with single strand binding protein (SSB) (Sigma-Aldrich), p7 or the storage buffer, incubated for 10 min at 30°C and resolved by 6% native PAGE (0.5× TBE) at room temperature.

    Article Title: Discovery of Dengue Virus NS4B Inhibitors
    Article Snippet: .. Micro BioSpin 6 columns (Bio-Rad) capable of separating free ligands from bound ligands were used to measure the binding of 3 H-labeled compound 14a to purified proteins. .. A binding mixture (20 μl) containing 20 mM sodium phosphate (pH 6.5), 0.1% 1-myristoyl-2-hydroxy- sn -glycero-3-[phospho-Rac-(1-glycerol)] (LMPG), 1 mM dithiothreitol (DTT), 150 mM NaCl, 5 to 50 μM NS4B, and 100 to 500 μM 3 H-labeled compound 14a was incubated at room temperature for 10 min, adsorbed to a Micro BioSpin column, and centrifuged for 1 min.

    Nucleic Acid Electrophoresis:

    Article Title: Direct observation of TALE protein dynamics reveals a two-state search mechanism
    Article Snippet: The purified ( > 90% purity by SDS–polyacrylamide gel electrophoresis) TAL effector proteins were buffer exchanged using Amicon Ultra 0.5 ml centrifugal units (EMD Millipore) and concentrated to 100–300 μM in 30 μl of labelling buffer (250 mM potassium phosphate pH 6, 500 mM KCl (Fisher), 5 mM dithiothreitol (Roche)). .. The labelled proteins were diluted with 400 μl fluorescence anisotropy buffer (20 μM Tris-HCl, pH 7.5, 100 mM Nacl, 0.5 mM EDTA (Fisher)) and purified from unreacted Cy3 by two consecutive passages through Micro Bio-spin 6 columns (Bio-Rad) following the manufacturer's instructions.

    Fluorescence:

    Article Title: Direct observation of TALE protein dynamics reveals a two-state search mechanism
    Article Snippet: .. The labelled proteins were diluted with 400 μl fluorescence anisotropy buffer (20 μM Tris-HCl, pH 7.5, 100 mM Nacl, 0.5 mM EDTA (Fisher)) and purified from unreacted Cy3 by two consecutive passages through Micro Bio-spin 6 columns (Bio-Rad) following the manufacturer's instructions. ..

    Isolation:

    Article Title: Antisense Transcription in Gammaretroviruses as a Mechanism of Insertional Activation of Host Genes ▿
    Article Snippet: Total RNA was isolated as described above, and 10 μg was subjected to Northern blot analyses using capillary transfer under the conditions described in reference . .. Residual nucleotides were removed with Micro Bio-Spin 6 columns (Bio-Rad) before being used for hybridization with buffers and conditions described in reference .

    Transmission Assay:

    Article Title: Structures of DPAGT1 Explain Glycosylation Disease Mechanisms and Advance TB Antibiotic Design
    Article Snippet: Native mass spectrometry For native mass spectrometry analysis purified DPAGT1 protein was diluted to 10 μM protein concentration using 200 mM ammonium acetate supplemented with 0.16% OGNG solution followed by buffer exchanged into 200 mM ammonium acetate, 0.16% OGNG using a micro biospin column (Micro Bio-Spin 6, Bio-Rad). .. Native MS experiments were conducted using a Q Exactive instrument (Thermo Fisher, Germany) with modifications for high-mass transmission optimization.

    Size-exclusion Chromatography:

    Article Title: Hsp70 Oligomerization Is Mediated by an Interaction between the Interdomain Linker and the Substrate-Binding Domain
    Article Snippet: Mass spectrometry Prior to MS analysis, samples were buffer exchanged into 150 mM ammonium acetate, pH 7.4, using Micro Bio-spin 6 columns (Bio-Rad, Digilab Division, Cambridge, MA, USA). .. In the case of the substrate binding experiments, proteins were analyzed in the presence of a 1: 1 molar ratio of the NR peptide; this substrate concentration, lower than in the SEC experiments, was chosen to ensure adequate resolution of the various charge state series, which is critical for unambiguous assignment of substrate-bound states.

    Labeling:

    Article Title: Dynamic active site protection by the M. tuberculosis protein tyrosine phosphatase PtpB lid domain
    Article Snippet: Paragraph title: PtpB Labeling ... To remove PO4 from the sample, the protein was separated four times on Micro Bio-Spin™ 6 columns (BioRad) equilibrated in 20 mM Tris-HCl pH 7.5, 100 mM NaCl.

    Purification:

    Article Title: Structures of DPAGT1 Explain Glycosylation Disease Mechanisms and Advance TB Antibiotic Design
    Article Snippet: .. Native mass spectrometry For native mass spectrometry analysis purified DPAGT1 protein was diluted to 10 μM protein concentration using 200 mM ammonium acetate supplemented with 0.16% OGNG solution followed by buffer exchanged into 200 mM ammonium acetate, 0.16% OGNG using a micro biospin column (Micro Bio-Spin 6, Bio-Rad). .. Native MS experiments were conducted using a Q Exactive instrument (Thermo Fisher, Germany) with modifications for high-mass transmission optimization.

    Article Title: Direct observation of TALE protein dynamics reveals a two-state search mechanism
    Article Snippet: .. The labelled proteins were diluted with 400 μl fluorescence anisotropy buffer (20 μM Tris-HCl, pH 7.5, 100 mM Nacl, 0.5 mM EDTA (Fisher)) and purified from unreacted Cy3 by two consecutive passages through Micro Bio-spin 6 columns (Bio-Rad) following the manufacturer's instructions. ..

    Article Title: Discovery of Dengue Virus NS4B Inhibitors
    Article Snippet: .. Micro BioSpin 6 columns (Bio-Rad) capable of separating free ligands from bound ligands were used to measure the binding of 3 H-labeled compound 14a to purified proteins. .. A binding mixture (20 μl) containing 20 mM sodium phosphate (pH 6.5), 0.1% 1-myristoyl-2-hydroxy- sn -glycero-3-[phospho-Rac-(1-glycerol)] (LMPG), 1 mM dithiothreitol (DTT), 150 mM NaCl, 5 to 50 μM NS4B, and 100 to 500 μM 3 H-labeled compound 14a was incubated at room temperature for 10 min, adsorbed to a Micro BioSpin column, and centrifuged for 1 min.

    Protein Purification:

    Article Title: Direct observation of TALE protein dynamics reveals a two-state search mechanism
    Article Snippet: Paragraph title: Protein purification and labelling ... The labelled proteins were diluted with 400 μl fluorescence anisotropy buffer (20 μM Tris-HCl, pH 7.5, 100 mM Nacl, 0.5 mM EDTA (Fisher)) and purified from unreacted Cy3 by two consecutive passages through Micro Bio-spin 6 columns (Bio-Rad) following the manufacturer's instructions.

    Electrophoretic Mobility Shift Assay:

    Article Title: Bacteriophage Xp10 anti-termination factor p7 induces forward translocation by host RNA polymerase
    Article Snippet: .. Electrophoretic mobility shift assay DNA substrates were 5′ end-labelled with with γ[32 P]ATP and PNK, and unincorporated γ[32 P]ATP was removed by gel filtration on Micro-BioSpin 6 columns (BioRad). .. Radiolabelled DNA were mixed with single strand binding protein (SSB) (Sigma-Aldrich), p7 or the storage buffer, incubated for 10 min at 30°C and resolved by 6% native PAGE (0.5× TBE) at room temperature.

    Sampling:

    Article Title: Structural disorder and induced folding within two cereal, ABA stress and ripening (ASR) proteins
    Article Snippet: Prior to analysis, all proteins were buffer-exchanged into 250 mM ammonium acetate, pH 8.0 using Micro Bio-SpinTM 6 Columns (Bio-Rad). .. The capillary voltage was set to 1.8–2.1 kV, the source temperature was 30 °C, the sampling cone was set to 150 V, the source offset to 150 V, and the trap gas flow to 4 mL/min.

    Concentration Assay:

    Article Title: Hsp70 Oligomerization Is Mediated by an Interaction between the Interdomain Linker and the Substrate-Binding Domain
    Article Snippet: Mass spectrometry Prior to MS analysis, samples were buffer exchanged into 150 mM ammonium acetate, pH 7.4, using Micro Bio-spin 6 columns (Bio-Rad, Digilab Division, Cambridge, MA, USA). .. In the case of the substrate binding experiments, proteins were analyzed in the presence of a 1: 1 molar ratio of the NR peptide; this substrate concentration, lower than in the SEC experiments, was chosen to ensure adequate resolution of the various charge state series, which is critical for unambiguous assignment of substrate-bound states.

    Article Title: Screening of candidate substrates and coupling ions of transporters by thermostability shift assays
    Article Snippet: Lipids were re-hydrated in 20 mM MES pH 6.5, 50 mM NaCl and, when required, compound was added to a final concentration of 20 mM from a 200 mM stock. .. Bio-beads were removed by passage of the sample through empty micro-bio spin columns (Bio-Rad, Hemel Hempstead, UK).

    Variant Assay:

    Article Title: Dynamic active site protection by the M. tuberculosis protein tyrosine phosphatase PtpB lid domain
    Article Snippet: Each PtpB cysteine variant was labeled simultaneously with the dyes Alexa Fluor® C2 maleimide 555 (FRET donor dye, Invitrogen) and Alexa Fluor® C2 maleimide 647 (FRET acceptor, Invitrogen). .. To remove PO4 from the sample, the protein was separated four times on Micro Bio-Spin™ 6 columns (BioRad) equilibrated in 20 mM Tris-HCl pH 7.5, 100 mM NaCl.

    Clear Native PAGE:

    Article Title: Bacteriophage Xp10 anti-termination factor p7 induces forward translocation by host RNA polymerase
    Article Snippet: Electrophoretic mobility shift assay DNA substrates were 5′ end-labelled with with γ[32 P]ATP and PNK, and unincorporated γ[32 P]ATP was removed by gel filtration on Micro-BioSpin 6 columns (BioRad). .. Radiolabelled DNA were mixed with single strand binding protein (SSB) (Sigma-Aldrich), p7 or the storage buffer, incubated for 10 min at 30°C and resolved by 6% native PAGE (0.5× TBE) at room temperature.

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  • 99
    Bio-Rad micro biospin 6 columns
    Binding of 3 H-labeled compound 14a to recombinant NS4B. (A) SDS-PAGE analysis of recombinant DENV-2 NS4A and NS4B proteins. Full-length NS4A and the N-terminal 125 amino acids of DENV-2 NS4B were expressed and purified by using an E. coli ). The molecular masses (kilodaltons) of protein standards (marker) are indicated on the left and right. (B) Gel filtration analysis of binding of 3 H-labeled compound 14a to the NS4B protein. 3 H-labeled compound 14a was mixed with the indicated proteins and centrifuged through a Micro <t>BioSpin</t> 6 gel filtration column. Protein-bound 3 H-labeled compound-14a was quantitated in the eluent by using a beta scintillation counter (PerkinElmer Life Sciences). NS4A served as a negative control. Analysis of each data point was carried out in triplicate. ***, P
    Micro Biospin 6 Columns, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/micro biospin 6 columns/product/Bio-Rad
    Average 99 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    micro biospin 6 columns - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    99
    Bio-Rad size exclusion micro bio spin p 6 pre packed column
    Gel-filtration analysis of AF and HMAF-inactivated TrxR. TrxR (80 nM) was first incubated with NADPH (100 μM) at 25 °C for 10 min followed by the addition of test compounds and further incubation for 2 h at 25 °C (A, AF, 3.3, 6.7, 16.7, 33.3, 66.7 μM; B, HMAF, 0.13, 0.27, 0.53, 1.33, 2.67 μM). Unbound compound was removed by Micro Bio-Spin™ P-6 pre-packed size exclusion columns. The residual activity was measured with DTNB assay described in the experimental details.
    Size Exclusion Micro Bio Spin P 6 Pre Packed Column, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/size exclusion micro bio spin p 6 pre packed column/product/Bio-Rad
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    size exclusion micro bio spin p 6 pre packed column - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    92
    Bio-Rad ns1 glycoprotein micro bio spin p 6 gel columns
    Gel-filtration analysis of AF and HMAF-inactivated TrxR. TrxR (80 nM) was first incubated with NADPH (100 μM) at 25 °C for 10 min followed by the addition of test compounds and further incubation for 2 h at 25 °C (A, AF, 3.3, 6.7, 16.7, 33.3, 66.7 μM; B, HMAF, 0.13, 0.27, 0.53, 1.33, 2.67 μM). Unbound compound was removed by Micro Bio-Spin™ P-6 pre-packed size exclusion columns. The residual activity was measured with DTNB assay described in the experimental details.
    Ns1 Glycoprotein Micro Bio Spin P 6 Gel Columns, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ns1 glycoprotein micro bio spin p 6 gel columns/product/Bio-Rad
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ns1 glycoprotein micro bio spin p 6 gel columns - by Bioz Stars, 2020-04
    92/100 stars
      Buy from Supplier

    Image Search Results


    Binding of 3 H-labeled compound 14a to recombinant NS4B. (A) SDS-PAGE analysis of recombinant DENV-2 NS4A and NS4B proteins. Full-length NS4A and the N-terminal 125 amino acids of DENV-2 NS4B were expressed and purified by using an E. coli ). The molecular masses (kilodaltons) of protein standards (marker) are indicated on the left and right. (B) Gel filtration analysis of binding of 3 H-labeled compound 14a to the NS4B protein. 3 H-labeled compound 14a was mixed with the indicated proteins and centrifuged through a Micro BioSpin 6 gel filtration column. Protein-bound 3 H-labeled compound-14a was quantitated in the eluent by using a beta scintillation counter (PerkinElmer Life Sciences). NS4A served as a negative control. Analysis of each data point was carried out in triplicate. ***, P

    Journal: Journal of Virology

    Article Title: Discovery of Dengue Virus NS4B Inhibitors

    doi: 10.1128/JVI.00855-15

    Figure Lengend Snippet: Binding of 3 H-labeled compound 14a to recombinant NS4B. (A) SDS-PAGE analysis of recombinant DENV-2 NS4A and NS4B proteins. Full-length NS4A and the N-terminal 125 amino acids of DENV-2 NS4B were expressed and purified by using an E. coli ). The molecular masses (kilodaltons) of protein standards (marker) are indicated on the left and right. (B) Gel filtration analysis of binding of 3 H-labeled compound 14a to the NS4B protein. 3 H-labeled compound 14a was mixed with the indicated proteins and centrifuged through a Micro BioSpin 6 gel filtration column. Protein-bound 3 H-labeled compound-14a was quantitated in the eluent by using a beta scintillation counter (PerkinElmer Life Sciences). NS4A served as a negative control. Analysis of each data point was carried out in triplicate. ***, P

    Article Snippet: Micro BioSpin 6 columns (Bio-Rad) capable of separating free ligands from bound ligands were used to measure the binding of 3 H-labeled compound 14a to purified proteins.

    Techniques: Binding Assay, Labeling, Recombinant, SDS Page, Purification, Marker, Filtration, Negative Control

    Transfer of phosphate from CheA1- 32 P (top panels) and CheA2- 32 P (bottom panels) to CheY3. CheA1 and CheA2 were autophosphorylated with [ 32 P]ATP, and unincorporated [ 32 P]ATP was removed by centrifugation on Bio-Spin6 columns. Recovered CheA1- 32 P and CheA2-

    Journal: Journal of Bacteriology

    Article Title: CheY3 of Borrelia burgdorferi Is the Key Response Regulator Essential for Chemotaxis and Forms a Long-Lived Phosphorylated Intermediate ▿

    doi: 10.1128/JB.00362-11

    Figure Lengend Snippet: Transfer of phosphate from CheA1- 32 P (top panels) and CheA2- 32 P (bottom panels) to CheY3. CheA1 and CheA2 were autophosphorylated with [ 32 P]ATP, and unincorporated [ 32 P]ATP was removed by centrifugation on Bio-Spin6 columns. Recovered CheA1- 32 P and CheA2-

    Article Snippet: Micro Bio-Spin6 columns were obtained from Bio-Rad; all other reagents were obtained from Sigma-Aldrich Chemical Co. rCheA1 and rCheA2 (2 μM) were incubated in 50 mM Tris (pH 8.5)–50 mM KCl,–5 mM MgCl2 –0.3 mM ATP–1 μCi [γ-32 P]ATP (6,000 Ci/mmol)/μl for 30 min.

    Techniques: Centrifugation

    Gel-filtration analysis of AF and HMAF-inactivated TrxR. TrxR (80 nM) was first incubated with NADPH (100 μM) at 25 °C for 10 min followed by the addition of test compounds and further incubation for 2 h at 25 °C (A, AF, 3.3, 6.7, 16.7, 33.3, 66.7 μM; B, HMAF, 0.13, 0.27, 0.53, 1.33, 2.67 μM). Unbound compound was removed by Micro Bio-Spin™ P-6 pre-packed size exclusion columns. The residual activity was measured with DTNB assay described in the experimental details.

    Journal: Chemical research in toxicology

    Article Title: Susceptibility of the antioxidant selenoenyzmes thioredoxin reductase and glutathione peroxidase to alkylation-mediated inhibition by anticancer acylfulvenes

    doi: 10.1021/tx2000152

    Figure Lengend Snippet: Gel-filtration analysis of AF and HMAF-inactivated TrxR. TrxR (80 nM) was first incubated with NADPH (100 μM) at 25 °C for 10 min followed by the addition of test compounds and further incubation for 2 h at 25 °C (A, AF, 3.3, 6.7, 16.7, 33.3, 66.7 μM; B, HMAF, 0.13, 0.27, 0.53, 1.33, 2.67 μM). Unbound compound was removed by Micro Bio-Spin™ P-6 pre-packed size exclusion columns. The residual activity was measured with DTNB assay described in the experimental details.

    Article Snippet: After the 2 h reaction period, unbound drug was removed by gel-filtration with a size-exclusion Micro Bio-Spin™ P-6 pre-packed column (containing 10 mM Tris-HCl buffer, pH 7.4, with 0.02% sodium azide (w/v)) according to the Bio-Rad’s protocols.

    Techniques: Filtration, Incubation, Activity Assay, DTNB Assay