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Millipore mgso4
Mgso4, supplied by Millipore, used in various techniques. Bioz Stars score: 98/100, based on 447 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mgso4/product/Millipore
Average 98 stars, based on 447 article reviews
Price from $9.99 to $1999.99
mgso4 - by Bioz Stars, 2020-01
98/100 stars

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Centrifugation:

Article Title: Structure of the Alternative Complex III in a supercomplex with cytochrome oxidase
Article Snippet: Membrane preparation and protein purification Cells grown overnight were collected by centrifugation (14,000 × g for 10 min). .. The cell pellet from 12 L of culture (~2.5 g/L) was resuspended in ~200 ml of 20 mM Tris-HCl buffer, pH 8 (buffer A) with 5 mM MgSO4 , DNAse I (Sigma) and a protease inhibitor cocktail (Sigma).

Amplification:

Article Title: Rapid Detection of Pseudomonas aeruginosa and Acinetobacter baumannii Harboring blaVIM-2, blaIMP-1 and blaOXA-23 Genes by Using Loop-Mediated Isothermal Amplification Methods
Article Snippet: The primer sets (shown in ) amplified 147 bp (89-235), 187 bp (116-302), and 191 bp (136-326) target sequences of bla VIM-2 , bla IMP-1 , and bla OXA-23 between the F2 and B2 regions, respectively ( ). .. To determine the optimal concentration of MgSO4 , 10× LAMP buffer was made as described previously [ ] containing various concentrations of MgSO4 (Sigma-Aldrich Co., St. Louis, MO, USA).

SYBR Green Assay:

Article Title: Detection of Bar Transgenic Sugarcane with a Rapid and Visual Loop-Mediated Isothermal Amplification Assay
Article Snippet: The initial LAMP was carried out in a 25.0 μL mixture containing 2.5 μL 10 × ThermoPol Reaction Buffer [New England Biolabs, Massachusetts, USA, including 20.0 mM Tris-HCl (pH 8.8), 10.0 mM KCl, 2.0 mM MgSO4 , 10.0 mM (NH4 )2 SO4 , 0.1% Triton X-100], 3.75 mM MgSO4 (50.0 mM, Sigma-Aldrich Inc., St. Louis, USA), 0.8 μM each FIP and BIP, 0.2 μM each F3 and B3, 0.4 μM each LF and LB, 1.4 mM dNTPs (10.0 mM, TaKaRa Biotechnology Co., Ltd., Dalian, China), 8.0 U Bst DNA polymerase large fragment (New England Biolabs, Massachusetts, USA) and a specified amount of sugarcane genomic DNA or plasmid 1Ac0229. .. Real-time LAMP reaction assays were performed on an ABI 7500 System (Applied Biosystems, Foster, USA) using final volumes of 25.0 μL, consisting of 0.5 × SYBR Green I (Bio-tek Co., Ltd., Beijing, China) except the LAMP reaction mixture mentioned above with the following conditions in isotherm model and FAM: 65°C for 70 min.

Incubation:

Article Title: Detection of Bar Transgenic Sugarcane with a Rapid and Visual Loop-Mediated Isothermal Amplification Assay
Article Snippet: The initial LAMP was carried out in a 25.0 μL mixture containing 2.5 μL 10 × ThermoPol Reaction Buffer [New England Biolabs, Massachusetts, USA, including 20.0 mM Tris-HCl (pH 8.8), 10.0 mM KCl, 2.0 mM MgSO4 , 10.0 mM (NH4 )2 SO4 , 0.1% Triton X-100], 3.75 mM MgSO4 (50.0 mM, Sigma-Aldrich Inc., St. Louis, USA), 0.8 μM each FIP and BIP, 0.2 μM each F3 and B3, 0.4 μM each LF and LB, 1.4 mM dNTPs (10.0 mM, TaKaRa Biotechnology Co., Ltd., Dalian, China), 8.0 U Bst DNA polymerase large fragment (New England Biolabs, Massachusetts, USA) and a specified amount of sugarcane genomic DNA or plasmid 1Ac0229. .. The mixture was incubated at 65°C for 60 min, followed by heating at 80°C for 5 min to inactivate the Bst DNA enzyme and terminate the reaction.

Article Title: Residual Detergent Detection Method for Nondestructive Cytocompatibility Evaluation of Decellularized Whole Lung Scaffolds
Article Snippet: Following a 5, 15, 30, or 60 min incubation period at room temperature, 150 μL of the bottom chloroform layer was extracted ( ; Supplementary Data are available online at ) and the absorbance was measured in a Synergy HT Multi-Detection Microplate Reader (Biotek Instruments, Winooski, VT). .. To analyze the effect of other ions and reagents commonly utilized during decellularization protocols, , , , , , , , , we determined the SDC concentration in the presence of a range of Triton X-100 (0–1%), 2 mM CaCl2 (Sigma-Aldrich), 1.3 mM MgSO4 (Sigma-Aldrich), a solution of both 2 mM CaCl2 (Sigma-Aldrich) and 1.3 mM MgSO4 (Sigma-Aldrich), or 1 M NaCl (Fisher Scientific, Hampton, NH).

Article Title: Crosstalk between lysine methylation and phosphorylation of ATG16L1 dictates the apoptosis of hypoxia/reoxygenation-induced cardiomyocytes
Article Snippet: Setd7 -null and Setd7 wild-type mouse embryonic fibroblasts (MEFs) were incubated in DMEM supplemented with 1 × nonessential amino acids (Sigma-Aldrich, D6046), sodium pyruvate (110 mg/l), 10% fetal bovine serum, and 1% penicillin–streptomycin. .. Briefly, whole hearts from 2- to 5-d-old rats were isolated and rinsed in a balanced salt solution (137 mM NaCl, 0.338 mM NaH2 PO4 , 1.26 mM CaCl2 , 0.6 U/mL of pancreatin [Sigma-Aldrich, P7545], 5.5 mM MgSO4 , 5.3 mM KCl, 4.17 mM NaHCO3 , 0.8 mM MgSO4 , 0.44 mM KH2 PO4 , pH 7.3-7.4), with collagenase (95 U/mL; Sigma-Aldrich, C0130) 5 times.

Article Title: Respiratory Syncytial Virus-Infected Mesenchymal Stem Cells Regulate Immunity via Interferon Beta and Indoleamine-2,3-Dioxygenase
Article Snippet: Viral pellet was washed and resuspended in precooled buffer: 0.1M MgSO4 , 150mM NaCl and 50mM HEPES (Sigma-Aldrich, St. Louis, MO, USA). .. Cells were grown to confluence in 24-well plates, washed and incubated for 90 minutes with serial dilutions of purified virus stock in Opti-MEM with 2% FBS.

Infection:

Article Title: Aspergillus fumigatus Inhibits Pseudomonas aeruginosa in Co-culture: Implications of a Mutually Antagonistic Relationship on Virulence and Inflammation in the CF Airway
Article Snippet: Paragraph title: Virulence of Co-colonizing Pathogens in the Galleria mellonella Acute Infection Model ... P. aeruginosa strains were cultured in LB broth for 24 h at 37°C, diluted to OD600nm 0.6, pelleted and resuspended and 10-fold serial dilutions prepared of the neat sample (2 × 107 CFU/ml) in 10 mM MgSO4 (Sigma, Wicklow, Ireland) and 50 μl aliquots spread on blood agar to confirm the bioburden.

Article Title: Respiratory Syncytial Virus-Infected Mesenchymal Stem Cells Regulate Immunity via Interferon Beta and Indoleamine-2,3-Dioxygenase
Article Snippet: For visualization of viral infection in lentiviral-transduced cells a recombinant green fluorescent protein-expressing RSV was used (generously provided by Dr. Mark Peeples, Nationwide Children’s Hospital, Columbus, OH) [ ]. .. Viral pellet was washed and resuspended in precooled buffer: 0.1M MgSO4 , 150mM NaCl and 50mM HEPES (Sigma-Aldrich, St. Louis, MO, USA).

Expressing:

Article Title: Respiratory Syncytial Virus-Infected Mesenchymal Stem Cells Regulate Immunity via Interferon Beta and Indoleamine-2,3-Dioxygenase
Article Snippet: Respiratory syncytial virus strain and PFU assay The strain of RSV used in all except the lentiviral experiments was a recombinant A2 strain expressing a red fluorescent marker, mKate2, as well as the F protein from the clinical strain Line 19 (rA2-KL19F) [ , ]. .. Viral pellet was washed and resuspended in precooled buffer: 0.1M MgSO4 , 150mM NaCl and 50mM HEPES (Sigma-Aldrich, St. Louis, MO, USA).

Derivative Assay:

Article Title: Crosstalk between lysine methylation and phosphorylation of ATG16L1 dictates the apoptosis of hypoxia/reoxygenation-induced cardiomyocytes
Article Snippet: H9c2 cardiomyocytes derived from rat myocardium and HEK293T were obtained (ATCC, CRL-1446, CRL-3216) and cultured in DMEM with 10% fetal bovine serum without penicillin/streptomycin. .. Briefly, whole hearts from 2- to 5-d-old rats were isolated and rinsed in a balanced salt solution (137 mM NaCl, 0.338 mM NaH2 PO4 , 1.26 mM CaCl2 , 0.6 U/mL of pancreatin [Sigma-Aldrich, P7545], 5.5 mM MgSO4 , 5.3 mM KCl, 4.17 mM NaHCO3 , 0.8 mM MgSO4 , 0.44 mM KH2 PO4 , pH 7.3-7.4), with collagenase (95 U/mL; Sigma-Aldrich, C0130) 5 times.

Protease Inhibitor:

Article Title: Structure of the Alternative Complex III in a supercomplex with cytochrome oxidase
Article Snippet: .. The cell pellet from 12 L of culture (~2.5 g/L) was resuspended in ~200 ml of 20 mM Tris-HCl buffer, pH 8 (buffer A) with 5 mM MgSO4 , DNAse I (Sigma) and a protease inhibitor cocktail (Sigma). ..

Cell Culture:

Article Title: Aspergillus fumigatus Inhibits Pseudomonas aeruginosa in Co-culture: Implications of a Mutually Antagonistic Relationship on Virulence and Inflammation in the CF Airway
Article Snippet: .. P. aeruginosa strains were cultured in LB broth for 24 h at 37°C, diluted to OD600nm 0.6, pelleted and resuspended and 10-fold serial dilutions prepared of the neat sample (2 × 107 CFU/ml) in 10 mM MgSO4 (Sigma, Wicklow, Ireland) and 50 μl aliquots spread on blood agar to confirm the bioburden. ..

Article Title: Crosstalk between lysine methylation and phosphorylation of ATG16L1 dictates the apoptosis of hypoxia/reoxygenation-induced cardiomyocytes
Article Snippet: Primary neonatal rat ventricular cardiomyocytes (NRVCs) were isolated and cultured as described previously. .. Briefly, whole hearts from 2- to 5-d-old rats were isolated and rinsed in a balanced salt solution (137 mM NaCl, 0.338 mM NaH2 PO4 , 1.26 mM CaCl2 , 0.6 U/mL of pancreatin [Sigma-Aldrich, P7545], 5.5 mM MgSO4 , 5.3 mM KCl, 4.17 mM NaHCO3 , 0.8 mM MgSO4 , 0.44 mM KH2 PO4 , pH 7.3-7.4), with collagenase (95 U/mL; Sigma-Aldrich, C0130) 5 times.

Imaging:

Article Title: Synchronous long-term oscillations in a synthetic gene circuit
Article Snippet: .. Cell preparation E. coli strains were grown overnight in LB with appropriate antibiotics and diluted 1:100 ~2–3 hours before the beginning of the experiments in imaging media, consisting of M9 salts, 10% (v/v) LB, 0.2% (w/v) glucose, 2 mM MgSO4 , 0.1 mM CaCl2 , 1.5 μ M thiamine hydrochloride and 0.85 g/L Pluronic F-108 (Sigma Aldrich, included as a passivating agent). ..

Polymerase Chain Reaction:

Article Title: Rapid Detection of Pseudomonas aeruginosa and Acinetobacter baumannii Harboring blaVIM-2, blaIMP-1 and blaOXA-23 Genes by Using Loop-Mediated Isothermal Amplification Methods
Article Snippet: To determine the optimal concentration of MgSO4 , 10× LAMP buffer was made as described previously [ ] containing various concentrations of MgSO4 (Sigma-Aldrich Co., St. Louis, MO, USA). .. Formation of a white precipitate (magnesium pyrophosphate [Mg2 P2 O7 ]) after centrifuging the PCR tubes at 15,328g for 2 min was a criterion for a positive LAMP reaction.

Injection:

Article Title: Aspergillus fumigatus Inhibits Pseudomonas aeruginosa in Co-culture: Implications of a Mutually Antagonistic Relationship on Virulence and Inflammation in the CF Airway
Article Snippet: Virulence of Co-colonizing Pathogens in the Galleria mellonella Acute Infection Model In order to establish bioburden to be injected, 10-fold serial dilutions of A. fumigatus strains were prepared in PBS and conidia counted on MEA plates. .. P. aeruginosa strains were cultured in LB broth for 24 h at 37°C, diluted to OD600nm 0.6, pelleted and resuspended and 10-fold serial dilutions prepared of the neat sample (2 × 107 CFU/ml) in 10 mM MgSO4 (Sigma, Wicklow, Ireland) and 50 μl aliquots spread on blood agar to confirm the bioburden.

Recombinant:

Article Title: Bad Phages in Good Bacteria: Role of the Mysterious orf63 of λ and Shiga Toxin-Converting Φ24B Bacteriophages
Article Snippet: Media and growth conditions All in vivo experiments were performed in LB liquid medium (Sigma—Aldrich) supplemented with 10 mM MgSO4 (phage λ or phage λΔorf63 ) or with 10 mM MgSO4 and 10 mM CaCl2 (phage Φ24B or phage Φ24B Δorf63 ), and with 50 μg/ml ampicillin (if necessary) (Sigma—Aldrich). .. To stimulate Orf63 protein production from the recombinant pUC18 derivatives, overnight bacterial cultures were diluted 1:100 in fresh LB medium and treated with IPTG (A & A Biotechnology) to a final concentration of 1 mM.

Article Title: Respiratory Syncytial Virus-Infected Mesenchymal Stem Cells Regulate Immunity via Interferon Beta and Indoleamine-2,3-Dioxygenase
Article Snippet: For visualization of viral infection in lentiviral-transduced cells a recombinant green fluorescent protein-expressing RSV was used (generously provided by Dr. Mark Peeples, Nationwide Children’s Hospital, Columbus, OH) [ ]. .. Viral pellet was washed and resuspended in precooled buffer: 0.1M MgSO4 , 150mM NaCl and 50mM HEPES (Sigma-Aldrich, St. Louis, MO, USA).

In Vivo:

Article Title: Bad Phages in Good Bacteria: Role of the Mysterious orf63 of λ and Shiga Toxin-Converting Φ24B Bacteriophages
Article Snippet: .. Media and growth conditions All in vivo experiments were performed in LB liquid medium (Sigma—Aldrich) supplemented with 10 mM MgSO4 (phage λ or phage λΔorf63 ) or with 10 mM MgSO4 and 10 mM CaCl2 (phage Φ24B or phage Φ24B Δorf63 ), and with 50 μg/ml ampicillin (if necessary) (Sigma—Aldrich). .. To stimulate Orf63 protein production from the recombinant pUC18 derivatives, overnight bacterial cultures were diluted 1:100 in fresh LB medium and treated with IPTG (A & A Biotechnology) to a final concentration of 1 mM.

Isolation:

Article Title: Regeneration and Experimental Orthotopic Transplantation of a Bioengineered Kidney
Article Snippet: .. Isolated kidney experiments To assess in vitro kidney function, we perfused single native, regenerated, and decellularized kidneys with 0.22 μm-filtered (Fisher) Krebs-Henseleit (KH) solution containing: NaHCO3 (25.0 mM), NaCl (118 mM), KCl (4.7 mM), MgSO4 (1.2 mM), NaH2 PO4 (1.2 mM), CaCl2 (1.2 mM), BSA (5.0 g/dL), D-glucose (100 mg/dL), urea (12 mg/dL), creatinine (20 mg/dL), (Sigma Aldrich). .. We added amino acids glycine (750 mg/L), L-alanine (890 mg/L), L-asparagine (1,320 mg/L), L-aspartic acid (1330 mg/L), L-glutamic acid (1470 mg/L), L-proline (1150 mg/L), and L-serine (1050 mg/L) prior to testing (Invitrogen).

Article Title: Crosstalk between lysine methylation and phosphorylation of ATG16L1 dictates the apoptosis of hypoxia/reoxygenation-induced cardiomyocytes
Article Snippet: .. Briefly, whole hearts from 2- to 5-d-old rats were isolated and rinsed in a balanced salt solution (137 mM NaCl, 0.338 mM NaH2 PO4 , 1.26 mM CaCl2 , 0.6 U/mL of pancreatin [Sigma-Aldrich, P7545], 5.5 mM MgSO4 , 5.3 mM KCl, 4.17 mM NaHCO3 , 0.8 mM MgSO4 , 0.44 mM KH2 PO4 , pH 7.3-7.4), with collagenase (95 U/mL; Sigma-Aldrich, C0130) 5 times. .. Then the pellet was resuspended in buffer A (horse serum [Si gma-Aldrich, H1270]:M199 [Sigma-Aldrich, M-4530] mixed 4:1, streptomycin [100 µg/mL], fetal calf serum [5%; Sigma-Aldrich, F7524], and penicillin [100 U/mL]).

Microelectrode Array:

Article Title: Aspergillus fumigatus Inhibits Pseudomonas aeruginosa in Co-culture: Implications of a Mutually Antagonistic Relationship on Virulence and Inflammation in the CF Airway
Article Snippet: Virulence of Co-colonizing Pathogens in the Galleria mellonella Acute Infection Model In order to establish bioburden to be injected, 10-fold serial dilutions of A. fumigatus strains were prepared in PBS and conidia counted on MEA plates. .. P. aeruginosa strains were cultured in LB broth for 24 h at 37°C, diluted to OD600nm 0.6, pelleted and resuspended and 10-fold serial dilutions prepared of the neat sample (2 × 107 CFU/ml) in 10 mM MgSO4 (Sigma, Wicklow, Ireland) and 50 μl aliquots spread on blood agar to confirm the bioburden.

Purification:

Article Title: Respiratory Syncytial Virus-Infected Mesenchymal Stem Cells Regulate Immunity via Interferon Beta and Indoleamine-2,3-Dioxygenase
Article Snippet: Viral stocks were prepared by propagating the virus in HEp-2 cells (ATCC, Manassas, VA, USA) and purification by ultracentrifugation in a 30% glycerol, 0.1M MgSO4 and 50mM HEPES solution for 3 hours at 24,000×g. .. Viral pellet was washed and resuspended in precooled buffer: 0.1M MgSO4 , 150mM NaCl and 50mM HEPES (Sigma-Aldrich, St. Louis, MO, USA).

Protein Purification:

Article Title: Structure of the Alternative Complex III in a supercomplex with cytochrome oxidase
Article Snippet: Paragraph title: Membrane preparation and protein purification ... The cell pellet from 12 L of culture (~2.5 g/L) was resuspended in ~200 ml of 20 mM Tris-HCl buffer, pH 8 (buffer A) with 5 mM MgSO4 , DNAse I (Sigma) and a protease inhibitor cocktail (Sigma).

Bioburden Testing:

Article Title: Aspergillus fumigatus Inhibits Pseudomonas aeruginosa in Co-culture: Implications of a Mutually Antagonistic Relationship on Virulence and Inflammation in the CF Airway
Article Snippet: .. P. aeruginosa strains were cultured in LB broth for 24 h at 37°C, diluted to OD600nm 0.6, pelleted and resuspended and 10-fold serial dilutions prepared of the neat sample (2 × 107 CFU/ml) in 10 mM MgSO4 (Sigma, Wicklow, Ireland) and 50 μl aliquots spread on blood agar to confirm the bioburden. ..

Plasmid Preparation:

Article Title: Detection of Bar Transgenic Sugarcane with a Rapid and Visual Loop-Mediated Isothermal Amplification Assay
Article Snippet: .. The initial LAMP was carried out in a 25.0 μL mixture containing 2.5 μL 10 × ThermoPol Reaction Buffer [New England Biolabs, Massachusetts, USA, including 20.0 mM Tris-HCl (pH 8.8), 10.0 mM KCl, 2.0 mM MgSO4 , 10.0 mM (NH4 )2 SO4 , 0.1% Triton X-100], 3.75 mM MgSO4 (50.0 mM, Sigma-Aldrich Inc., St. Louis, USA), 0.8 μM each FIP and BIP, 0.2 μM each F3 and B3, 0.4 μM each LF and LB, 1.4 mM dNTPs (10.0 mM, TaKaRa Biotechnology Co., Ltd., Dalian, China), 8.0 U Bst DNA polymerase large fragment (New England Biolabs, Massachusetts, USA) and a specified amount of sugarcane genomic DNA or plasmid 1Ac0229. .. The mixture was incubated at 65°C for 60 min, followed by heating at 80°C for 5 min to inactivate the Bst DNA enzyme and terminate the reaction.

In Vitro:

Article Title: Regeneration and Experimental Orthotopic Transplantation of a Bioengineered Kidney
Article Snippet: .. Isolated kidney experiments To assess in vitro kidney function, we perfused single native, regenerated, and decellularized kidneys with 0.22 μm-filtered (Fisher) Krebs-Henseleit (KH) solution containing: NaHCO3 (25.0 mM), NaCl (118 mM), KCl (4.7 mM), MgSO4 (1.2 mM), NaH2 PO4 (1.2 mM), CaCl2 (1.2 mM), BSA (5.0 g/dL), D-glucose (100 mg/dL), urea (12 mg/dL), creatinine (20 mg/dL), (Sigma Aldrich). .. We added amino acids glycine (750 mg/L), L-alanine (890 mg/L), L-asparagine (1,320 mg/L), L-aspartic acid (1330 mg/L), L-glutamic acid (1470 mg/L), L-proline (1150 mg/L), and L-serine (1050 mg/L) prior to testing (Invitrogen).

Evaporation:

Article Title: Residual Detergent Detection Method for Nondestructive Cytocompatibility Evaluation of Decellularized Whole Lung Scaffolds
Article Snippet: To analyze the effect of other ions and reagents commonly utilized during decellularization protocols, , , , , , , , , we determined the SDC concentration in the presence of a range of Triton X-100 (0–1%), 2 mM CaCl2 (Sigma-Aldrich), 1.3 mM MgSO4 (Sigma-Aldrich), a solution of both 2 mM CaCl2 (Sigma-Aldrich) and 1.3 mM MgSO4 (Sigma-Aldrich), or 1 M NaCl (Fisher Scientific, Hampton, NH). .. To analyze the effect of other ions and reagents commonly utilized during decellularization protocols, , , , , , , , , we determined the SDC concentration in the presence of a range of Triton X-100 (0–1%), 2 mM CaCl2 (Sigma-Aldrich), 1.3 mM MgSO4 (Sigma-Aldrich), a solution of both 2 mM CaCl2 (Sigma-Aldrich) and 1.3 mM MgSO4 (Sigma-Aldrich), or 1 M NaCl (Fisher Scientific, Hampton, NH).

Concentration Assay:

Article Title: Lipid Production From Waste Materials in Seawater-Based Medium by the Yeast Yarrowia lipolytica
Article Snippet: An inoculum of 0.2 L was introduced into the bioreactor containing crude glycerol medium: fresh water, 150 g/L crude glycerol (187.5 g/L of 80% crude glycerol), 4.61 g/L (NH4 )2 SO4 in C/N ratio 60, 1 g/L yeast extract, 1 g/L MgSO4 × 7H2 O (FW-CG medium) or seawater, 150 g/L crude glycerol (187.5 g/L of 80% crude glycerol), 4.61 g/L (NH4 )2 SO4 in C/N ratio 60, 1 g/L yeast extract, 1 g/L MgSO4 × 7H2 O and 36 g/L of sea salts (Sigma-Aldrich) (SW-CG medium). .. The stirrer speed was adjusted to 800 rpm, and the dissolved oxygen concentration was maintained at 25 ± 5%.

Article Title: Bad Phages in Good Bacteria: Role of the Mysterious orf63 of λ and Shiga Toxin-Converting Φ24B Bacteriophages
Article Snippet: Media and growth conditions All in vivo experiments were performed in LB liquid medium (Sigma—Aldrich) supplemented with 10 mM MgSO4 (phage λ or phage λΔorf63 ) or with 10 mM MgSO4 and 10 mM CaCl2 (phage Φ24B or phage Φ24B Δorf63 ), and with 50 μg/ml ampicillin (if necessary) (Sigma—Aldrich). .. To stimulate Orf63 protein production from the recombinant pUC18 derivatives, overnight bacterial cultures were diluted 1:100 in fresh LB medium and treated with IPTG (A & A Biotechnology) to a final concentration of 1 mM.

Article Title: Residual Detergent Detection Method for Nondestructive Cytocompatibility Evaluation of Decellularized Whole Lung Scaffolds
Article Snippet: .. To analyze the effect of other ions and reagents commonly utilized during decellularization protocols, , , , , , , , , we determined the SDC concentration in the presence of a range of Triton X-100 (0–1%), 2 mM CaCl2 (Sigma-Aldrich), 1.3 mM MgSO4 (Sigma-Aldrich), a solution of both 2 mM CaCl2 (Sigma-Aldrich) and 1.3 mM MgSO4 (Sigma-Aldrich), or 1 M NaCl (Fisher Scientific, Hampton, NH). .. To assess the effect of PBS on assay performance, SDC and SDS standards were prepared in PBS in addition to DI water.

Article Title: Rapid Detection of Pseudomonas aeruginosa and Acinetobacter baumannii Harboring blaVIM-2, blaIMP-1 and blaOXA-23 Genes by Using Loop-Mediated Isothermal Amplification Methods
Article Snippet: .. To determine the optimal concentration of MgSO4 , 10× LAMP buffer was made as described previously [ ] containing various concentrations of MgSO4 (Sigma-Aldrich Co., St. Louis, MO, USA). ..

Marker:

Article Title: Respiratory Syncytial Virus-Infected Mesenchymal Stem Cells Regulate Immunity via Interferon Beta and Indoleamine-2,3-Dioxygenase
Article Snippet: Respiratory syncytial virus strain and PFU assay The strain of RSV used in all except the lentiviral experiments was a recombinant A2 strain expressing a red fluorescent marker, mKate2, as well as the F protein from the clinical strain Line 19 (rA2-KL19F) [ , ]. .. Viral pellet was washed and resuspended in precooled buffer: 0.1M MgSO4 , 150mM NaCl and 50mM HEPES (Sigma-Aldrich, St. Louis, MO, USA).

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    Millipore magnesium sulfate
    Magnesium Sulfate, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 57 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/magnesium sulfate/product/Millipore
    Average 90 stars, based on 57 article reviews
    Price from $9.99 to $1999.99
    magnesium sulfate - by Bioz Stars, 2020-01
    90/100 stars
      Buy from Supplier

    90
    Millipore magnesium sulfate heptahydrate
    Magnesium Sulfate Heptahydrate, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/magnesium sulfate heptahydrate/product/Millipore
    Average 90 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    magnesium sulfate heptahydrate - by Bioz Stars, 2020-01
    90/100 stars
      Buy from Supplier

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