mgso4  (Millipore)


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  • 91
    Name:
    Sucrose
    Description:

    Catalog Number:
    s2395
    Price:
    None
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    Structured Review

    Millipore mgso4
    Sucrose

    https://www.bioz.com/result/mgso4/product/Millipore
    Average 91 stars, based on 164 article reviews
    Price from $9.99 to $1999.99
    mgso4 - by Bioz Stars, 2020-07
    91/100 stars

    Images

    1) Product Images from "Insights into the Molecular Basis of L-Form Formation and Survival in Escherichia coli"

    Article Title: Insights into the Molecular Basis of L-Form Formation and Survival in Escherichia coli

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0007316

    Comparison of L-form E. coli and classical E. coli morphologies. A E. coli colony on L-form induction media (LIM) exhibiting typical “fried egg” morphology. B Classical E. coli colony on Brain Heart Infusion (BHI) agar. C E. coli colony on BHI+ 10% Sucrose and 0.125% MgSO4 (BHI+ sucrose control media). D E. coli penicillin G mutant colony on BHI+ Pen G. E Phase contrast of rod-shaped E. coli cells within a classical colony. Scale bar, 3 µm. F Phase contrast of coccoid cells within E. coli L-form colony agar squash. Scale bar, 3 µm. G Individual coccoid cells in soft agar LIM. Scale bar, 10 µm. H Transmission electron microscopy (TEM) photo of a coccoid cell within an L-form colony. Scale bar, 200 nm. All images are by Hoffman modulation and scale bars are 100 µm unless specified.
    Figure Legend Snippet: Comparison of L-form E. coli and classical E. coli morphologies. A E. coli colony on L-form induction media (LIM) exhibiting typical “fried egg” morphology. B Classical E. coli colony on Brain Heart Infusion (BHI) agar. C E. coli colony on BHI+ 10% Sucrose and 0.125% MgSO4 (BHI+ sucrose control media). D E. coli penicillin G mutant colony on BHI+ Pen G. E Phase contrast of rod-shaped E. coli cells within a classical colony. Scale bar, 3 µm. F Phase contrast of coccoid cells within E. coli L-form colony agar squash. Scale bar, 3 µm. G Individual coccoid cells in soft agar LIM. Scale bar, 10 µm. H Transmission electron microscopy (TEM) photo of a coccoid cell within an L-form colony. Scale bar, 200 nm. All images are by Hoffman modulation and scale bars are 100 µm unless specified.

    Techniques Used: Mutagenesis, Transmission Assay, Electron Microscopy, Transmission Electron Microscopy

    Related Articles

    In Vivo:

    Article Title: JAK2 is dispensable for maintenance of JAK2 mutant B-cell acute lymphoblastic leukemias
    Article Snippet: .. For shRNA induction in vivo, mice received Dox-supplemented drinking water (2 mg/mL with 2% sucrose; Sigma-Aldrich) and 625 mg/kg food (Specialty Feeds) for the specified time points. .. Transplanted mice were monitored weekly for leukemia development by weekly analysis of circulating leukemia cells (by flow cytometry) and assessment of total WBCs (Sysmex hematology analyzer, Sysmex Corporation) from peripheral blood.

    Article Title: The aged niche disrupts muscle stem cell quiescence
    Article Snippet: .. IN-VIVO CELL DIVISION ANALYSIS To assess cell proliferation and label retention character aged and adult C57BL/6, Ctrl, Spry1flx/flx and Spry1OX/OX mice were fed BrdU (Sigma) (0.5 mg/ml supplemented with 5% sucrose) continuously for 6 weeks. .. For label retention studies, Spry1flx/flx mice were given 3 daily intraperitoneal (IP) injections of Tmx (diluted in corn oil (Sigma)) following BrdU loading and placed on regular drinking water to chase label.

    shRNA:

    Article Title: JAK2 is dispensable for maintenance of JAK2 mutant B-cell acute lymphoblastic leukemias
    Article Snippet: .. For shRNA induction in vivo, mice received Dox-supplemented drinking water (2 mg/mL with 2% sucrose; Sigma-Aldrich) and 625 mg/kg food (Specialty Feeds) for the specified time points. .. Transplanted mice were monitored weekly for leukemia development by weekly analysis of circulating leukemia cells (by flow cytometry) and assessment of total WBCs (Sysmex hematology analyzer, Sysmex Corporation) from peripheral blood.

    Caspase Assay:

    Article Title: Independent roles of the priming and the triggering of the NLRP3 inflammasome in the heart
    Article Snippet: .. Briefly, proteins were extracted from frozen hearts and spleens using RIPA buffer (Sigma Aldrich, St Louis, MO, USA) and were diluted in caspase assay buffer (31% sucrose, 3.1% HEPES, and 0.31% CHAPS; Sigma Aldrich). .. The activity was measured using a fluorometric substrate YVAD-AFC (Enzo Life Sciences, Farmingdale, NY, USA), after subtraction of background signal in the presence of the caspase-1 inhibitor YVAD-CHO (Enzo Life Sciences).

    Mouse Assay:

    Article Title: JAK2 is dispensable for maintenance of JAK2 mutant B-cell acute lymphoblastic leukemias
    Article Snippet: .. For shRNA induction in vivo, mice received Dox-supplemented drinking water (2 mg/mL with 2% sucrose; Sigma-Aldrich) and 625 mg/kg food (Specialty Feeds) for the specified time points. .. Transplanted mice were monitored weekly for leukemia development by weekly analysis of circulating leukemia cells (by flow cytometry) and assessment of total WBCs (Sysmex hematology analyzer, Sysmex Corporation) from peripheral blood.

    Article Title: The aged niche disrupts muscle stem cell quiescence
    Article Snippet: .. IN-VIVO CELL DIVISION ANALYSIS To assess cell proliferation and label retention character aged and adult C57BL/6, Ctrl, Spry1flx/flx and Spry1OX/OX mice were fed BrdU (Sigma) (0.5 mg/ml supplemented with 5% sucrose) continuously for 6 weeks. .. For label retention studies, Spry1flx/flx mice were given 3 daily intraperitoneal (IP) injections of Tmx (diluted in corn oil (Sigma)) following BrdU loading and placed on regular drinking water to chase label.

    Incubation:

    Article Title: Light-sheet microscopy imaging of a whole cleared rat brain with Thy1-GFP transgene
    Article Snippet: .. Next for further clearing, samples were incubated in CUBIC Reagent 2 (50 wt% sucrose, 25 wt% urea, 10 wt% 2,20,20′-nitrilotriethanol (#90279, Sigma), and 0.1% (vol/vol) Triton X-100 for 1 week (whole brain) or 2 days in RT. .. Light-sheet imaging of CUBIC-cleared samples was performed using microscopy immersion oil (Cargille FF, RI 1.479) as the mounting solution.

    other:

    Article Title: Insights into the Molecular Basis of L-Form Formation and Survival in Escherichia coli
    Article Snippet: Undiluted along with serial tenfold dilutions of cells were spread (100 µl) or spotted (10 µl) onto L-form induction media (LIM) which consisted of brain heart infusion broth (BHI) Becton Dickinson (BD) supplemented with 1% agar (BD), 10% sucrose, 0.125% MgSO4 , and 6000 µg (10,000 units )/ml of Penicillin G (Sigma).

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  • 97
    Millipore spermine nonoate
    Effects of nitrosative stress on the viability of C. jejuni . Growing cultures of C. jejuni NCTC 11168 (▴) and CJCGB01 (○) were challenged with the indicated concentrations of GSNO (a), SNP (b), and spermine <t>NONOate</t> (c) for 1 h and then
    Spermine Nonoate, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/spermine nonoate/product/Millipore
    Average 97 stars, based on 16 article reviews
    Price from $9.99 to $1999.99
    spermine nonoate - by Bioz Stars, 2020-07
    97/100 stars
      Buy from Supplier

    Image Search Results


    Effects of nitrosative stress on the viability of C. jejuni . Growing cultures of C. jejuni NCTC 11168 (▴) and CJCGB01 (○) were challenged with the indicated concentrations of GSNO (a), SNP (b), and spermine NONOate (c) for 1 h and then

    Journal: Journal of Bacteriology

    Article Title: Role of an Inducible Single-Domain Hemoglobin in Mediating Resistance to Nitric Oxide and Nitrosative Stress in Campylobacter jejuni and Campylobacter coli

    doi: 10.1128/JB.186.16.5332-5341.2004

    Figure Lengend Snippet: Effects of nitrosative stress on the viability of C. jejuni . Growing cultures of C. jejuni NCTC 11168 (▴) and CJCGB01 (○) were challenged with the indicated concentrations of GSNO (a), SNP (b), and spermine NONOate (c) for 1 h and then

    Article Snippet: In contrast, spermine NONOate directly liberates NO with a specified half-life (230 min; Calbiochem data) and can be used to study the effect of NO per se.

    Techniques:

    Effect of spermine nitric oxide donor on intracellular signalling. Skeletal muscle strips from seven healthy participants were incubated in the absence (basal) or presence of spermine NONOate (Spe-NO, nitric oxide donor; 5 mmol/l) or insulin (120 nmol/l). a Representative immunoblots of protein phosphorylation and abundance. GAPDH, glyceraldehyde-3-phosphate dehydrogenase. Bar graphs show quantification for ( b ) pAkt (Ser 473 ), ( c ) pTBC1D1/D4, ( d ) pGSK3α/β (Ser 21/9 ) and ( e ) pCaMK II (Thr 286 ). Results ( b – e ) are mean ± SEM arbitrary units. ** p

    Journal: Diabetologia

    Article Title: Nitric oxide increases cyclic GMP levels, AMP-activated protein kinase (AMPK)?1-specific activity and glucose transport in human skeletal muscle

    doi: 10.1007/s00125-010-1716-x

    Figure Lengend Snippet: Effect of spermine nitric oxide donor on intracellular signalling. Skeletal muscle strips from seven healthy participants were incubated in the absence (basal) or presence of spermine NONOate (Spe-NO, nitric oxide donor; 5 mmol/l) or insulin (120 nmol/l). a Representative immunoblots of protein phosphorylation and abundance. GAPDH, glyceraldehyde-3-phosphate dehydrogenase. Bar graphs show quantification for ( b ) pAkt (Ser 473 ), ( c ) pTBC1D1/D4, ( d ) pGSK3α/β (Ser 21/9 ) and ( e ) pCaMK II (Thr 286 ). Results ( b – e ) are mean ± SEM arbitrary units. ** p

    Article Snippet: Thus, muscle strips were exposed to either spermine NONOate or insulin for a total of 60 min. At the end of the incubation protocol, skeletal muscle specimens were blotted of excess fluid, snap-frozen in liquid nitrogen and stored at −80°C until further analysis.

    Techniques: Incubation, Western Blot

    Effect of the guanylate cyclase inhibitor LY-83583 on spermine NONOate induced-glycogen synthesis and AMPK phosphorylation. L6 myotubes were treated with spermine NONOate (Spe-NO, nitric oxide donor; 20 µmol/l) and incubated in the absence or presence of LY-835883 (10 µmol/l). Glycogen synthesis ( n = 5–8) ( a ) and AMPK Thr 172 phosphorylation ( n = 5–6) ( b ) were determined. Results are reported as mean ± SEM. Glucose incorporation into glycogen is expressed as percentage of basal. c Protein content of the AMPK-α1 and AMPK-α2 subunits was determined in L6 myotubes. *** p

    Journal: Diabetologia

    Article Title: Nitric oxide increases cyclic GMP levels, AMP-activated protein kinase (AMPK)?1-specific activity and glucose transport in human skeletal muscle

    doi: 10.1007/s00125-010-1716-x

    Figure Lengend Snippet: Effect of the guanylate cyclase inhibitor LY-83583 on spermine NONOate induced-glycogen synthesis and AMPK phosphorylation. L6 myotubes were treated with spermine NONOate (Spe-NO, nitric oxide donor; 20 µmol/l) and incubated in the absence or presence of LY-835883 (10 µmol/l). Glycogen synthesis ( n = 5–8) ( a ) and AMPK Thr 172 phosphorylation ( n = 5–6) ( b ) were determined. Results are reported as mean ± SEM. Glucose incorporation into glycogen is expressed as percentage of basal. c Protein content of the AMPK-α1 and AMPK-α2 subunits was determined in L6 myotubes. *** p

    Article Snippet: Thus, muscle strips were exposed to either spermine NONOate or insulin for a total of 60 min. At the end of the incubation protocol, skeletal muscle specimens were blotted of excess fluid, snap-frozen in liquid nitrogen and stored at −80°C until further analysis.

    Techniques: Incubation

    Effect of spermine NONOate and insulin on glucose metabolism and intracellular signalling in L6 myotubes. Myotubes were treated with spermine NONOate (Spe-NO, nitric oxide donor; 20 µmol/l), in the absence (white bars) or presence (black bars) of insulin. a Glucose incorporation into glycogen ( n = 13–15) and ( b ) glucose uptake ( n = 10–15) were determined. Results are expressed as mean ± SEM percentage of basal values. Signal transduction was also determined for ( c ) pAMPK (Thr 172 ), ( d ) pAkt (Ser 473 ), ( e ) pACC (Ser 227 ) and ( f ) pTBC1D1/D4 ( n = 5–7). Results are mean ± SEM arbitrary units. * p

    Journal: Diabetologia

    Article Title: Nitric oxide increases cyclic GMP levels, AMP-activated protein kinase (AMPK)?1-specific activity and glucose transport in human skeletal muscle

    doi: 10.1007/s00125-010-1716-x

    Figure Lengend Snippet: Effect of spermine NONOate and insulin on glucose metabolism and intracellular signalling in L6 myotubes. Myotubes were treated with spermine NONOate (Spe-NO, nitric oxide donor; 20 µmol/l), in the absence (white bars) or presence (black bars) of insulin. a Glucose incorporation into glycogen ( n = 13–15) and ( b ) glucose uptake ( n = 10–15) were determined. Results are expressed as mean ± SEM percentage of basal values. Signal transduction was also determined for ( c ) pAMPK (Thr 172 ), ( d ) pAkt (Ser 473 ), ( e ) pACC (Ser 227 ) and ( f ) pTBC1D1/D4 ( n = 5–7). Results are mean ± SEM arbitrary units. * p

    Article Snippet: Thus, muscle strips were exposed to either spermine NONOate or insulin for a total of 60 min. At the end of the incubation protocol, skeletal muscle specimens were blotted of excess fluid, snap-frozen in liquid nitrogen and stored at −80°C until further analysis.

    Techniques: Transduction

    Effect of spermine nitric oxide donor on glucose transport, cGMP content and AMPK activity in human skeletal muscle. Skeletal muscle strips from seven healthy men were incubated in the absence (basal) or presence of spermine NONOate (Spe-NO, nitric oxide donor; 5 mmol/l) or insulin (120 nmol/l). Glucose transport ( a ), intracellular cyclic GMP (cGMP) content ( b ), and both AMPK-α1 ( c ) and AMPK-α2 ( d ) isoform-specific activity were determined. e AMPK-α1 and AMPK-α2 ( f ) isoform-specific activity in skeletal muscle from healthy men ( n = 2) in response to acute exercise. Results are expressed as mean ± SEM. * p

    Journal: Diabetologia

    Article Title: Nitric oxide increases cyclic GMP levels, AMP-activated protein kinase (AMPK)?1-specific activity and glucose transport in human skeletal muscle

    doi: 10.1007/s00125-010-1716-x

    Figure Lengend Snippet: Effect of spermine nitric oxide donor on glucose transport, cGMP content and AMPK activity in human skeletal muscle. Skeletal muscle strips from seven healthy men were incubated in the absence (basal) or presence of spermine NONOate (Spe-NO, nitric oxide donor; 5 mmol/l) or insulin (120 nmol/l). Glucose transport ( a ), intracellular cyclic GMP (cGMP) content ( b ), and both AMPK-α1 ( c ) and AMPK-α2 ( d ) isoform-specific activity were determined. e AMPK-α1 and AMPK-α2 ( f ) isoform-specific activity in skeletal muscle from healthy men ( n = 2) in response to acute exercise. Results are expressed as mean ± SEM. * p

    Article Snippet: Thus, muscle strips were exposed to either spermine NONOate or insulin for a total of 60 min. At the end of the incubation protocol, skeletal muscle specimens were blotted of excess fluid, snap-frozen in liquid nitrogen and stored at −80°C until further analysis.

    Techniques: Activity Assay, Incubation