mgcl2  (Jena Bioscience)


Bioz Verified Symbol Jena Bioscience is a verified supplier
Bioz Manufacturer Symbol Jena Bioscience manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95
    Name:
    dNTP Bundle
    Description:
    dNTP Bundle contains four separate solutions of ultrapure dATP dCTP dGTP and dTTP supplied as clear aqueous solutions pH 8 5 dNTP || Cat No || cap color dATP || NU 1001 || red dCTP || NU 1002 || blue dGTP || NU 1003 || yellow dTTP || NU 1004 || green
    Catalog Number:
    nu-1005l
    Price:
    218.24
    Purity:
    ≥ 99 % (HPLC)
    Category:
    Nucleotides Nucleosides
    Buy from Supplier


    Structured Review

    Jena Bioscience mgcl2
    dNTP Bundle contains four separate solutions of ultrapure dATP dCTP dGTP and dTTP supplied as clear aqueous solutions pH 8 5 dNTP || Cat No || cap color dATP || NU 1001 || red dCTP || NU 1002 || blue dGTP || NU 1003 || yellow dTTP || NU 1004 || green
    https://www.bioz.com/result/mgcl2/product/Jena Bioscience
    Average 95 stars, based on 75 article reviews
    Price from $9.99 to $1999.99
    mgcl2 - by Bioz Stars, 2020-09
    95/100 stars

    Images

    Related Articles

    Polymerase Chain Reaction:

    Article Title: Maternal DNA lineages at the gate of Europe in the 10th century AD
    Article Snippet: .. PCR reactions were set up as follows: 2–4 μl DNA extract, 1.25 U MangoTaq™ DNA Polymerase (Bioline Reagents Ltd, London, UK), 1x MangoTaq™ Colored Reaction Buffer, 2.5 mM MgCl2 , 0.2 mM dNTP mix, 0.5 μM each primer, and PCR-grade water (Jena Bioscience, Jena, Germany) up to 25 μl. .. The applied thermocycling conditions were: initial denaturation at 95°C for 5 min, 35 amplification cycles consisting of three steps (denaturation at 95°C for 30 sec, annealing at 46–56°C depending on the primer pair, for 30 sec and extension at 72°C for 30 sec) and final elongation at 72°C for 5 min. PCR products were checked on 1.5% agarose gel and then purified using FavorPrep GEL/ PCR Purification Kit (Favorgen Biotech Corp., Pingtung, Taiwan), according to the manufacturer’s protocol.

    Article Title: Detection, Virulence Gene Assessment and Antibiotic Resistance Pattern of O157 Enterohemorrhagic Escherichia coli in Tabriz, Iran
    Article Snippet: .. The final concentrations of the reaction components were as follows: 2 ng of DNA template, 1x PCR buffer, 0.2 mM of each dNTP, 2 mM of MgCl2 , 0.2 µM of each primer, and 1 U of Taq DNA polymerase (Jena Bioscience, Germany). ..

    Sequencing:

    Article Title: PolDIP2 interacts with human PrimPol and enhances its DNA polymerase activities
    Article Snippet: .. DNA primase assays Reactions were assembled in buffer containing 10 mM Bis–Tris–propane–HCl (pH 7.0), 10 mM MgCl2 , and 100 μM DTT, supplemented with 2 μM PrimPol, 250 μM dNTPs, 2.5 μM FAM dNTPs (dATP, dCTP, dUTP) (Jena-Biosciences), 1 μM single-stranded template (sequence 7, Supplementary Table S1), and varying concentrations of PolDIP2 or GST (as indicated on individual figures). .. Reactions were incubated at 37°C for 15 min before quenching in binding-washing (B-W) buffer (10 mM Tris–HCl pH 7.5, 500 mM NaCl, 10 mM EDTA).

    Incubation:

    Article Title: Repriming by PrimPol is critical for DNA replication restart downstream of lesions and chain-terminating nucleosides
    Article Snippet: .. Briefly, PrimPol (1 μM) was incubated at 37°C for 15 mins in 20 μl reactions volumes containing 10 mM Bis-Tris-Propane-HCl pH 7.0, 10 mM MgCl2 , 1 mM DTT, 250 μM dNTPs or rNTPs, and 2.5 μM FAM dNTPs (dATP, dCTP, dUTP) (Jena-Biosciences). .. Reactions were quenched with binding-washing buffer (B-W) buffer (10 mM Tris-HCl pH 7.5, 500 mM NaCl, 10 mM EDTA) and supplemented with ∼20 μl streptavidin coated beads.

    Amplification:

    Article Title: In Vitro Bioactivity of Methanolic Extracts from Amphipterygium adstringens (Schltdl.) Schiede ex Standl., Chenopodium ambrosioides L., Cirsium mexicanum DC., Eryngium carlinae F. Delaroche, and Pithecellobium dulce (Roxb.) Benth. Used in Traditional Medicine in Mexico
    Article Snippet: .. Amplification reactions were performed in a final volume of 20 μ l containing 20 mM Tris-HCl (pH 8.4), 50 mM KCl, 2 mM MgCl2 , 0.2 mM dNTPs mix, 1 μ M each primer, 10 ng of genomic DNA and 2 units of Taq DNA polymerase (Jena Bioscience, Jena, Germany). .. Amplifications were performed in a Multigene Thermocycler (Labnet International, Edison, USA) using the program: 1 step at 94°C for 5 min, 35 cycles consisting of 30 s at 94°C, 1 min at 52°C, and 1 min at 72°C and a final extension step of 5 min at 72°C.

    other:

    Article Title: De novo design of a synthetic riboswitch that regulates transcription termination
    Article Snippet: Chemicals Oligonucleotides were purchased from biomers.net, and dNTP were obtained from Jena Biosciences.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93
    Jena Bioscience maba atp
    MANF inhibits nucleotide exchange and <t>ATP-induced</t> substrate release from BiP. a Representative plot of fluorescence against time of pre-formed complexes of <t>MABA-ADP</t> and BiP NBD (1.25 µM) challenged at t = 0 with buffer solution containing ATP (125 µM) and MANF or its SAP domain (200 µM). b Bar diagram of MABA-ADP release rates from BiP NBD in the presence of MANF, the isolated SAP or SAPLIP domain or the indicated mutant forms of MANF. Bars represent mean values ± SD from three to five independent experiments (**** P
    Maba Atp, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/maba atp/product/Jena Bioscience
    Average 93 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    maba atp - by Bioz Stars, 2020-09
    93/100 stars
      Buy from Supplier

    95
    Jena Bioscience atp
    Eight structural motifs of Flavivirus NS3 helicases are highly conserved. The structural Motifs (I, Ia, II, III, IV, IVa, V, VI) are located between the <t>ATP</t> binding pocket and <t>RNA</t> binding cleft. Each Motif is highlighted as follows: Motif I in dark green; Motif Ia in blue; Motif II in orange; Motif III in royal blue; Motif IV in purple; Motif IVa in magenta; Motif V in lime green; Motif VI in red.
    Atp, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 95/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/atp/product/Jena Bioscience
    Average 95 stars, based on 19 article reviews
    Price from $9.99 to $1999.99
    atp - by Bioz Stars, 2020-09
    95/100 stars
      Buy from Supplier

    93
    Jena Bioscience fluorescein 12 atp
    Fluorescence polarization assay development and validation. A. Chemical structure of <t>8[(4-amino)butyl]-amino-ATP-MNT</t> (MABA-ATP). B. Chemical structure of <t>fluorescein-12-ATP</t> (F12-ATP). C. 0.5 μM MABA-ATP and 5 nM F12-ATP were titrated against various concentrations of GST-HEPN. D. 0.5 μM MABA-ATP and 5 nM F12-ATP and 120 nM / 7 μM GST-HEPN were titrated against various concentrations of GTP to verify that the probes bind to HEPN at its nucleotide-binding site.
    Fluorescein 12 Atp, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fluorescein 12 atp/product/Jena Bioscience
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fluorescein 12 atp - by Bioz Stars, 2020-09
    93/100 stars
      Buy from Supplier

    Image Search Results


    MANF inhibits nucleotide exchange and ATP-induced substrate release from BiP. a Representative plot of fluorescence against time of pre-formed complexes of MABA-ADP and BiP NBD (1.25 µM) challenged at t = 0 with buffer solution containing ATP (125 µM) and MANF or its SAP domain (200 µM). b Bar diagram of MABA-ADP release rates from BiP NBD in the presence of MANF, the isolated SAP or SAPLIP domain or the indicated mutant forms of MANF. Bars represent mean values ± SD from three to five independent experiments (**** P

    Journal: Nature Communications

    Article Title: MANF antagonizes nucleotide exchange by the endoplasmic reticulum chaperone BiP

    doi: 10.1038/s41467-019-08450-4

    Figure Lengend Snippet: MANF inhibits nucleotide exchange and ATP-induced substrate release from BiP. a Representative plot of fluorescence against time of pre-formed complexes of MABA-ADP and BiP NBD (1.25 µM) challenged at t = 0 with buffer solution containing ATP (125 µM) and MANF or its SAP domain (200 µM). b Bar diagram of MABA-ADP release rates from BiP NBD in the presence of MANF, the isolated SAP or SAPLIP domain or the indicated mutant forms of MANF. Bars represent mean values ± SD from three to five independent experiments (**** P

    Article Snippet: Nucleotide release kinetics of the fluorescent nucleotide analogue MABA-ADP (Jena Bioscience, cat. # NU-893-MNT) and nucleotide binding of MABA-ATP (Jena Bioscience, cat. # NU-806-MNT) were performed in a Perkin Elmer LS55 fluorescence spectrometer instrument (excitation 360 nm, emission 420 nm).

    Techniques: Fluorescence, Isolation, Mutagenesis

    Eight structural motifs of Flavivirus NS3 helicases are highly conserved. The structural Motifs (I, Ia, II, III, IV, IVa, V, VI) are located between the ATP binding pocket and RNA binding cleft. Each Motif is highlighted as follows: Motif I in dark green; Motif Ia in blue; Motif II in orange; Motif III in royal blue; Motif IV in purple; Motif IVa in magenta; Motif V in lime green; Motif VI in red.

    Journal: bioRxiv

    Article Title: Motif V acts as a Regulator of Energy Transduction Between the Flavivirus NS3 ATPase and RNA Binding Cleft

    doi: 10.1101/842088

    Figure Lengend Snippet: Eight structural motifs of Flavivirus NS3 helicases are highly conserved. The structural Motifs (I, Ia, II, III, IV, IVa, V, VI) are located between the ATP binding pocket and RNA binding cleft. Each Motif is highlighted as follows: Motif I in dark green; Motif Ia in blue; Motif II in orange; Motif III in royal blue; Motif IV in purple; Motif IVa in magenta; Motif V in lime green; Motif VI in red.

    Article Snippet: RNA Helicase Unwinding Activity Assay For the ATP dependent helicase assay( , ), all reactions were performed in 96-well black microplates (Thermo Scientific) in a final volume of 120 μL and they contained 50 nM NS3h, 0.05 mM TCEP, 0.01% Tween20, 5 μg/μL BSA, 1.25 mM MgCl2 , 25 mM MOPS (pH 6.5), 5 nM RNA complex, and varying concentrations of ATP (Jena Bioscience) ranging from 1 µM to 1 mM.

    Techniques: Binding Assay, RNA Binding Assay

    ATP dependency of ATP waiting times measured by GNP tracking and fluorescent ATP experiments. a A plot of the ATP binding rate against ATP concentrations. The ATP binding rate was fit to a straight line with a slope of 0.0038 ± 0.0001 s −1 nM −1 and an intercept of 0.17 ± 0.07 s −1 . The ATP binding rates indicated by green were calculated from the GNP tracking experiments. The ATP binding rates indicated by red were calculated from the fluorescent ATP turnover 53 , 54 . See Methods for details. Error bars indicate standard deviations. The points were obtained by the cumulative frequency plots in Supplementary Fig. 6 . Data were obtained from at least three independent experiments for each ATP concentration. b A schematic of the ATP waiting time measurement and a representative time course of the fluorescence intensity. A scattering image of GNP was used to decide the position of the myosin head. At the position, the time course of the fluorescence intensity from Cy3-labeled ATP (Cy3ATP) was monitored. Each spike indicates a binding event of Cy3ATP to a myosin head. The time between spikes corresponds to ATP waiting time.

    Journal: Communications Biology

    Article Title: Direct visualization of human myosin II force generation using DNA origami-based thick filaments

    doi: 10.1038/s42003-019-0683-0

    Figure Lengend Snippet: ATP dependency of ATP waiting times measured by GNP tracking and fluorescent ATP experiments. a A plot of the ATP binding rate against ATP concentrations. The ATP binding rate was fit to a straight line with a slope of 0.0038 ± 0.0001 s −1 nM −1 and an intercept of 0.17 ± 0.07 s −1 . The ATP binding rates indicated by green were calculated from the GNP tracking experiments. The ATP binding rates indicated by red were calculated from the fluorescent ATP turnover 53 , 54 . See Methods for details. Error bars indicate standard deviations. The points were obtained by the cumulative frequency plots in Supplementary Fig. 6 . Data were obtained from at least three independent experiments for each ATP concentration. b A schematic of the ATP waiting time measurement and a representative time course of the fluorescence intensity. A scattering image of GNP was used to decide the position of the myosin head. At the position, the time course of the fluorescence intensity from Cy3-labeled ATP (Cy3ATP) was monitored. Each spike indicates a binding event of Cy3ATP to a myosin head. The time between spikes corresponds to ATP waiting time.

    Article Snippet: In the fluorescent ATP experiments, Cy3-labeled 2′/3′-O-(2-aminoethyl-carbamoyl)-adenosine-5′-triphosphate (Jena Bioscience) was added to the imaging buffer instead of ATP.

    Techniques: Binding Assay, Concentration Assay, Fluorescence, Labeling

    Fluorescence polarization assay development and validation. A. Chemical structure of 8[(4-amino)butyl]-amino-ATP-MNT (MABA-ATP). B. Chemical structure of fluorescein-12-ATP (F12-ATP). C. 0.5 μM MABA-ATP and 5 nM F12-ATP were titrated against various concentrations of GST-HEPN. D. 0.5 μM MABA-ATP and 5 nM F12-ATP and 120 nM / 7 μM GST-HEPN were titrated against various concentrations of GTP to verify that the probes bind to HEPN at its nucleotide-binding site.

    Journal: PLoS ONE

    Article Title: High-Throughput Screening for Ligands of the HEPN Domain of Sacsin

    doi: 10.1371/journal.pone.0137298

    Figure Lengend Snippet: Fluorescence polarization assay development and validation. A. Chemical structure of 8[(4-amino)butyl]-amino-ATP-MNT (MABA-ATP). B. Chemical structure of fluorescein-12-ATP (F12-ATP). C. 0.5 μM MABA-ATP and 5 nM F12-ATP were titrated against various concentrations of GST-HEPN. D. 0.5 μM MABA-ATP and 5 nM F12-ATP and 120 nM / 7 μM GST-HEPN were titrated against various concentrations of GTP to verify that the probes bind to HEPN at its nucleotide-binding site.

    Article Snippet: Fluorescently labeled ATP probes for FP assays N -methylanthraniloyl 8[(4-amino)butyl]-amino-ATP (MABA-ATP) and fluorescein-12-ATP (F12-ATP) were purchased from Jena Bioscience and PerkinElmer, respectively.

    Techniques: Fluorescence, Binding Assay