mgcl2 cacl2  (Millipore)


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  • 99
    Name:
    Dulbecco s Phosphate Buffered Saline
    Description:

    Catalog Number:
    d8537
    Price:
    None
    Applications:
    DPBS is a balanced salt solution (BSS) used for the handling and culturing of mammalian cells. DPBS is used to to irrigate, wash, and dilute mammalian cells. Phosphate buffering maintains the pH in the physiological range. Calcium and magnesium facilitate cell binding and clumping. DPBS without these ions can be used to wash and rinse suspended cells.
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    Structured Review

    Millipore mgcl2 cacl2

    https://www.bioz.com/result/mgcl2 cacl2/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mgcl2 cacl2 - by Bioz Stars, 2020-09
    99/100 stars

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    Related Articles

    Modification:

    Article Title: Adipose-derived mesenchymal stem cells from liposuction and resected fat are feasible sources for regenerative medicine
    Article Snippet: .. Materials and reagents Cell culture medium (Dulbecco’s modified eagle medium, 4500 mg/l glucose, 0.584 g/l l -glutamine, DMEM), fetal bovine serum (FBS), penicillin/streptomycin (P/S), trypsin–EDTA, and Dulbecco’s phosphate buffered saline (DPBS) were purchased from Sigma-Aldrich (St. Louis, Missouri, USA). .. Collagenase Type II and trypan blue were purchased from Biochrom/Merck (Darmstadt, Germany).

    Incubation:

    Article Title: Destabilization of spindle assembly checkpoint causes aneuploidy during meiosis II in murine post-ovulatory aged oocytes
    Article Snippet: .. This was followed by incubation in Alexa Fluor® 594-conjugated anti-goat IgG secondary antibody (1:500 dilution, Invitrogen) for 60 min. After washing thrice with D-PBS, the oocytes were incubated with mouse anti-α-Tubulin monoclonal antibody (1:500 dilution, Sigma) as a primary antibody for 60 min at room temperature, washed thrice with D-PBS, then incubated in fluorescein isothiocyanate (FITC)-conjugated anti-mouse IgG (1:100 dilution, Sigma) as a secondary antibody for 1 h at room temperature. .. Oocyte chromosomes were stained with 0.1 μg/ml 4',6-diamidino-2-phenylindole (DAPI, Sigma) for 30 min at 4°C.

    Article Title: Norwalk Virus-Like Particle Hemagglutination by Binding to H Histo-Blood Group Antigens
    Article Snippet: .. Neuraminidase from Arthrobacter ureafaciens (6 μU; Sigma) in Dulbecco's PBS (D-PBS) (Invitrogen) was combined with the RBCs and incubated for 60 min at 37°C. l -(Toslylamido-2-phenyl)ethyl chloromethyl ketone-treated trypsin (1.25 ng; Sigma) in D-PBS was added to the RBCs and incubated for 30 min at 37°C. .. Trypsin activity was stopped by adding 0.5 μg of soybean trypsin inhibitor (Sigma) to the reaction mixture.

    Cell Culture:

    Article Title: Adipose-derived mesenchymal stem cells from liposuction and resected fat are feasible sources for regenerative medicine
    Article Snippet: .. Materials and reagents Cell culture medium (Dulbecco’s modified eagle medium, 4500 mg/l glucose, 0.584 g/l l -glutamine, DMEM), fetal bovine serum (FBS), penicillin/streptomycin (P/S), trypsin–EDTA, and Dulbecco’s phosphate buffered saline (DPBS) were purchased from Sigma-Aldrich (St. Louis, Missouri, USA). .. Collagenase Type II and trypan blue were purchased from Biochrom/Merck (Darmstadt, Germany).

    Purification:

    Article Title: Gelatin Type A from Porcine Skin Used as Co-Initiator in a Radical Photo-Initiating System
    Article Snippet: .. Materials Gelatin (type A, Vetec reagent grade powder, gel strength = 300 g Bloom, Mn = 50,000–100,000) from porcine skin, poly(ethylene glycol) diacrylate (PEGDA, Mn = 700 g mol−1 ), camphorquinone (CQ, 97%) and Dulbecco′s phosphate buffered saline (DPBS, pH 7.0–7.3) were purchased from Sigma-Aldrich (Milano, Italy) and used as received without further purification. .. Deionized water (DIH2 O) was obtained from a reverse osmosis (RO) purification system.

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  • 99
    Millipore binding buffer
    Binding Buffer, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1259 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/binding buffer/product/Millipore
    Average 99 stars, based on 1259 article reviews
    Price from $9.99 to $1999.99
    binding buffer - by Bioz Stars, 2020-09
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    90
    Millipore cytokine homogenate buffer
    Leptin and <t>cytokine</t> levels (IL-6 and TNF- α ) after 4 and 6 weeks on the diets. Consistent with the weight gain and epididymal fat pad weights, leptin levels were significantly higher after 4 and 6 weeks in the HAGE-HF group compared to the other groups. Also consistent with the liver histology and presence of inflammation after 6 weeks on the diet, IL-6 and TNF- α levels were significantly higher in the HAGE-HF group after 6 weeks on the diet.
    Cytokine Homogenate Buffer, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cytokine homogenate buffer/product/Millipore
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cytokine homogenate buffer - by Bioz Stars, 2020-09
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    90
    Millipore annexin buffer
    Apoptosis, loss of ΔΨ m, and ROS production are caspase dependent. HeLa cells were treated with actinomycin D or UV (as indicated) in the presence or absence of 50 μM of zVAD-fmk, harvested, and stained with <t>annexin</t> V–FITC and PI to assess cell death, TMRE to measure ΔΨm, and 2-HE to measure ROS production, and then analyzed by flow cytometry.
    Annexin Buffer, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 90 stars, based on 8 article reviews
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    85
    Millipore oocyte ringers hco3
    Apoptosis, loss of ΔΨ m, and ROS production are caspase dependent. HeLa cells were treated with actinomycin D or UV (as indicated) in the presence or absence of 50 μM of zVAD-fmk, harvested, and stained with <t>annexin</t> V–FITC and PI to assess cell death, TMRE to measure ΔΨm, and 2-HE to measure ROS production, and then analyzed by flow cytometry.
    Oocyte Ringers Hco3, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/oocyte ringers hco3/product/Millipore
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    oocyte ringers hco3 - by Bioz Stars, 2020-09
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    Image Search Results


    Leptin and cytokine levels (IL-6 and TNF- α ) after 4 and 6 weeks on the diets. Consistent with the weight gain and epididymal fat pad weights, leptin levels were significantly higher after 4 and 6 weeks in the HAGE-HF group compared to the other groups. Also consistent with the liver histology and presence of inflammation after 6 weeks on the diet, IL-6 and TNF- α levels were significantly higher in the HAGE-HF group after 6 weeks on the diet.

    Journal: BioMed Research International

    Article Title: Advanced Glycation End Products Induce Obesity and Hepatosteatosis in CD-1 Wild-Type Mice

    doi: 10.1155/2016/7867852

    Figure Lengend Snippet: Leptin and cytokine levels (IL-6 and TNF- α ) after 4 and 6 weeks on the diets. Consistent with the weight gain and epididymal fat pad weights, leptin levels were significantly higher after 4 and 6 weeks in the HAGE-HF group compared to the other groups. Also consistent with the liver histology and presence of inflammation after 6 weeks on the diet, IL-6 and TNF- α levels were significantly higher in the HAGE-HF group after 6 weeks on the diet.

    Article Snippet: The remaining liver was washed with cold HBSS and suspended in cytokine homogenate buffer (150 mM NaCl, 15 mM Tris, 1 mM CaCl2 ·2H2 O, and 1 mM MgCl2 ·6H2 O, adjusted to pH 7.4) plus 100x protease inhibitor cocktail 1 (Calbiochem, La Jolla, California) to a total volume of 10 mL and homogenized on ice using a Polytron® Homogenizer (Kinematica Inc., Bohemia, NY).

    Techniques:

    Apoptosis, loss of ΔΨ m, and ROS production are caspase dependent. HeLa cells were treated with actinomycin D or UV (as indicated) in the presence or absence of 50 μM of zVAD-fmk, harvested, and stained with annexin V–FITC and PI to assess cell death, TMRE to measure ΔΨm, and 2-HE to measure ROS production, and then analyzed by flow cytometry.

    Journal: The Journal of Cell Biology

    Article Title: Caspase-mediated loss of mitochondrial function and generation of reactive oxygen species during apoptosis

    doi: 10.1083/jcb.200208089

    Figure Lengend Snippet: Apoptosis, loss of ΔΨ m, and ROS production are caspase dependent. HeLa cells were treated with actinomycin D or UV (as indicated) in the presence or absence of 50 μM of zVAD-fmk, harvested, and stained with annexin V–FITC and PI to assess cell death, TMRE to measure ΔΨm, and 2-HE to measure ROS production, and then analyzed by flow cytometry.

    Article Snippet: The pellet was resuspended in 30 μl of annexin buffer (Hepes 10 mM, NaCl 150 mM, KCl 5 mM, MgCl2 1 mM, CaCl2 1.8 mM) and then divided into three groups: one third each for ΔΨm measurement (as described above), ROS measurement using 2 μM of 2-HE in MIB buffer, and cell death measurement using annexin V–FITC (Calbiochem).

    Techniques: Staining, Flow Cytometry, Cytometry