mgcl2 cacl2  (Millipore)


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  • 95
    Name:
    Dulbecco s Phosphate Buffered Saline
    Description:

    Catalog Number:
    d8537
    Price:
    None
    Applications:
    DPBS is a balanced salt solution (BSS) used for the handling and culturing of mammalian cells. DPBS is used to to irrigate, wash, and dilute mammalian cells. Phosphate buffering maintains the pH in the physiological range. Calcium and magnesium facilitate cell binding and clumping. DPBS without these ions can be used to wash and rinse suspended cells.
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    Structured Review

    Millipore mgcl2 cacl2

    https://www.bioz.com/result/mgcl2 cacl2/product/Millipore
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mgcl2 cacl2 - by Bioz Stars, 2020-02
    95/100 stars

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    Related Articles

    Immunohistochemistry:

    Article Title: Development of a low-pressure microtargeting biolistic device for transfection of retinal explants
    Article Snippet: Immunofluorescence assays Immunohistochemistry was used to probe additional aspects of explant health. .. At the conclusion of experiments, explants were rinsed once in Dulbecco’s PBS (#D8537; Sigma-Aldrich Co. LLC) and fixed with 4% paraformaldehyde in PBS containing 4% sucrose for 1 h at room temperature.

    Centrifugation:

    Article Title: Increased prefrontal cortex interleukin-2 protein levels and shift in the peripheral T cell population in progressive supranuclear palsy patients
    Article Snippet: Briefly, blood was diluted 1:1 in Dulbecco’s PBS (DPBS; Sigma-Aldrich; #D8537) containing 2 mM EDTA (Sigma-Aldrich; #E9884) and 1:5 in a Ficoll-Paque PLUS gradient (GE Healthcare Life Science; #17144003). .. On the day of analysis, PBMCs were thawed at 37 °C for 10 min before washing in 10% FCS in RPMI-1640 followed by centrifugation at 200 g for 10 min. 200,000 cells were added to a well on a round bottom plate.

    Article Title: The novel toluidine sulphonamide EL102 shows pre-clinical in vitro and in vivo activity against prostate cancer and circumvents MDR1 resistance
    Article Snippet: Cell suspensions were combined with the medium in the appropriate 15-ml tubes, and cell pellets were collected by centrifugation at 1000 × g at 4 °C for 5 min using soft acceleration. .. Pellets were resuspended in 500 μ l ice-cold dPBS (Sigma, #D8537) and transferred to labelled 1.5-ml tubes.

    Article Title: Radiosensitivity in breast cancer assessed by the Comet and micronucleus assays
    Article Snippet: Blood sampling and isolation of cells PBMCs were separated from the heparinised blood samples by density-gradient centrifugation using Ficoll–Histopaque 1077 (Sigma 1077-1, Deisenhofen, Germany) according to the manufacturer's instructions. .. Peripheral blood mononuclear cells were washed twice with Ca2+ - and Mg2+ -free physiological phosphate-buffered saline (PBS; Sigma D-8537) and finally resuspended in the freezing medium containing RPMI 1640 (Sigma R-8758), 10% foetal calf serum and 10% dimethyl sulphoxide, and stored frozen in a liquid nitrogen until analysis.

    Purification:

    Article Title: Influence of environmental conditions on the attenuation of ricin toxin on surfaces
    Article Snippet: Testing was conducted with a single lot of commercially prepared purified ricin toxin (Cat. No. L-1090, Ricin communis agglutinin II, certified as 5 mg/mL protein concentration; Vector Laboratories, Burlingame, CA), which was stored at 2–8°C and used as received. .. Whole castor beans were de-husked and homogenized into a slurry, precipitated from the solution, dialyzed, and rinsed with sterile PBS (Cat #D8537 Sigma-Aldrich, St. Louis, MO).

    Cell Cycle Assay:

    Article Title: Downregulation of endometrial mesenchymal marker SUSD2 causes cell senescence and cell death in endometrial carcinoma cells
    Article Snippet: Paragraph title: Cell cycle analysis ... The supernatant was discarded and 1 ml of -20°C ice-cold ethanol (#20821.330, VWR Chemicals, USA): PBS (#D8537, Sigma) mixture (3:1) was added to the pellet during vortexing.

    Article Title: The novel toluidine sulphonamide EL102 shows pre-clinical in vitro and in vivo activity against prostate cancer and circumvents MDR1 resistance
    Article Snippet: Paragraph title: Sub-G1 and cell cycle analysis by flow cytometry ... Pellets were resuspended in 500 μ l ice-cold dPBS (Sigma, #D8537) and transferred to labelled 1.5-ml tubes.

    Isolation:

    Article Title: Toll-Like Receptor Ligands and Interferon-γ Synergize for Induction of Antitumor M1 Macrophages
    Article Snippet: The isolated bone marrow was passed through a cell strainer with 70-µm pores (#CLS431751-50EA, Sigma-Aldrich, St. Louis, MO, USA) and cultured in 15 mm non-tissue culture treated dishes (#734-2359, VWR, Radnos, PA, USA) in RPMI 1640 medium containing 30% L929-derived conditioned medium. .. Macrophages were harvested by incubation for 30 min at 4°C with cold PBS without CaCl2 and MgCl2 (#D8537, Sigma-Aldrich).

    Article Title: Radiosensitivity in breast cancer assessed by the Comet and micronucleus assays
    Article Snippet: Paragraph title: Blood sampling and isolation of cells ... Peripheral blood mononuclear cells were washed twice with Ca2+ - and Mg2+ -free physiological phosphate-buffered saline (PBS; Sigma D-8537) and finally resuspended in the freezing medium containing RPMI 1640 (Sigma R-8758), 10% foetal calf serum and 10% dimethyl sulphoxide, and stored frozen in a liquid nitrogen until analysis.

    Protein Concentration:

    Article Title: Influence of environmental conditions on the attenuation of ricin toxin on surfaces
    Article Snippet: Testing was conducted with a single lot of commercially prepared purified ricin toxin (Cat. No. L-1090, Ricin communis agglutinin II, certified as 5 mg/mL protein concentration; Vector Laboratories, Burlingame, CA), which was stored at 2–8°C and used as received. .. Whole castor beans were de-husked and homogenized into a slurry, precipitated from the solution, dialyzed, and rinsed with sterile PBS (Cat #D8537 Sigma-Aldrich, St. Louis, MO).

    Flow Cytometry:

    Article Title: Increased prefrontal cortex interleukin-2 protein levels and shift in the peripheral T cell population in progressive supranuclear palsy patients
    Article Snippet: Paragraph title: Flow cytometry ... Briefly, blood was diluted 1:1 in Dulbecco’s PBS (DPBS; Sigma-Aldrich; #D8537) containing 2 mM EDTA (Sigma-Aldrich; #E9884) and 1:5 in a Ficoll-Paque PLUS gradient (GE Healthcare Life Science; #17144003).

    Article Title: Downregulation of endometrial mesenchymal marker SUSD2 causes cell senescence and cell death in endometrial carcinoma cells
    Article Snippet: The supernatant was discarded and 1 ml of -20°C ice-cold ethanol (#20821.330, VWR Chemicals, USA): PBS (#D8537, Sigma) mixture (3:1) was added to the pellet during vortexing. .. The mixture was kept at -20°C overnight, the next day washed with PBS again, 250 μl PI mix containing 50 μg/ml PI (#P4864, Sigma) and 100 μg/ml RNase A (#R4642, Sigma) were added, incubated for 30 min at 37°C, and subjected to flow cytometry for cell cycle and death analysis.

    Article Title: Toll-Like Receptor Ligands and Interferon-γ Synergize for Induction of Antitumor M1 Macrophages
    Article Snippet: Macrophages were harvested by incubation for 30 min at 4°C with cold PBS without CaCl2 and MgCl2 (#D8537, Sigma-Aldrich). .. The purity of the cells was 99% when analyzed by flow cytometry using the macrophage markers CD11b (#101219, BioLegend, San Diego, CA, USA) and F4/80 (#123116, BioLegend), and the cells were then referred to as bone marrow derived macrophages (BMDMs).

    Article Title: The novel toluidine sulphonamide EL102 shows pre-clinical in vitro and in vivo activity against prostate cancer and circumvents MDR1 resistance
    Article Snippet: Paragraph title: Sub-G1 and cell cycle analysis by flow cytometry ... Pellets were resuspended in 500 μ l ice-cold dPBS (Sigma, #D8537) and transferred to labelled 1.5-ml tubes.

    Blocking Assay:

    Article Title: Development of a low-pressure microtargeting biolistic device for transfection of retinal explants
    Article Snippet: At the conclusion of experiments, explants were rinsed once in Dulbecco’s PBS (#D8537; Sigma-Aldrich Co. LLC) and fixed with 4% paraformaldehyde in PBS containing 4% sucrose for 1 h at room temperature. .. Retinas were then rinsed in PBS and blocked for 1 h in blocking solution (PBS containing 0.5% Triton-X 100, 1% BSA, and 10% normal goat serum).

    Mouse Assay:

    Article Title: Toll-Like Receptor Ligands and Interferon-γ Synergize for Induction of Antitumor M1 Macrophages
    Article Snippet: Femurs and tibiae of the hind legs from 8- to 12-week-old male and female C57BL/6NRj mice were harvested and flushed with RPMI 1640 medium containing 10% FBS under sterile conditions. .. Macrophages were harvested by incubation for 30 min at 4°C with cold PBS without CaCl2 and MgCl2 (#D8537, Sigma-Aldrich).

    Cytometry:

    Article Title: Increased prefrontal cortex interleukin-2 protein levels and shift in the peripheral T cell population in progressive supranuclear palsy patients
    Article Snippet: Paragraph title: Flow cytometry ... Briefly, blood was diluted 1:1 in Dulbecco’s PBS (DPBS; Sigma-Aldrich; #D8537) containing 2 mM EDTA (Sigma-Aldrich; #E9884) and 1:5 in a Ficoll-Paque PLUS gradient (GE Healthcare Life Science; #17144003).

    Article Title: Downregulation of endometrial mesenchymal marker SUSD2 causes cell senescence and cell death in endometrial carcinoma cells
    Article Snippet: The supernatant was discarded and 1 ml of -20°C ice-cold ethanol (#20821.330, VWR Chemicals, USA): PBS (#D8537, Sigma) mixture (3:1) was added to the pellet during vortexing. .. The mixture was kept at -20°C overnight, the next day washed with PBS again, 250 μl PI mix containing 50 μg/ml PI (#P4864, Sigma) and 100 μg/ml RNase A (#R4642, Sigma) were added, incubated for 30 min at 37°C, and subjected to flow cytometry for cell cycle and death analysis.

    Article Title: Toll-Like Receptor Ligands and Interferon-γ Synergize for Induction of Antitumor M1 Macrophages
    Article Snippet: Macrophages were harvested by incubation for 30 min at 4°C with cold PBS without CaCl2 and MgCl2 (#D8537, Sigma-Aldrich). .. The purity of the cells was 99% when analyzed by flow cytometry using the macrophage markers CD11b (#101219, BioLegend, San Diego, CA, USA) and F4/80 (#123116, BioLegend), and the cells were then referred to as bone marrow derived macrophages (BMDMs).

    Article Title: The novel toluidine sulphonamide EL102 shows pre-clinical in vitro and in vivo activity against prostate cancer and circumvents MDR1 resistance
    Article Snippet: Paragraph title: Sub-G1 and cell cycle analysis by flow cytometry ... Pellets were resuspended in 500 μ l ice-cold dPBS (Sigma, #D8537) and transferred to labelled 1.5-ml tubes.

    Concentration Assay:

    Article Title: A rapid, gentle and scalable method for dissociation and fluorescent sorting of imaginal disc cells for mRNA sequencing
    Article Snippet: Collect the discs in a second glass dish containing Dulbecco's PBS (Sigma #D8537-500ML) placed on ice, up to 100 discs per dish. .. Add 100 μL TrypLE undiluted from its 10x working concentration.

    Incubation:

    Article Title: Development of a low-pressure microtargeting biolistic device for transfection of retinal explants
    Article Snippet: At the conclusion of experiments, explants were rinsed once in Dulbecco’s PBS (#D8537; Sigma-Aldrich Co. LLC) and fixed with 4% paraformaldehyde in PBS containing 4% sucrose for 1 h at room temperature. .. Tissues were incubated overnight in primary antibody solution (PBS containing 0.3% Triton-X 100, 0.5% BSA, and 10% normal goat serum) containing primary antibodies against green fluorescent protein (GFP; 1:1,500; Abcam, Cambridge, MA), glial fibrillary acidic protein (GFAP; 1:1,000; Millipore, Billerica, MA), or Smi-31 (1:1,000; Covance, Princeton, NJ), as indicated.

    Article Title: Thrombin modifies growth, proliferation and apoptosis of human colon organoids: a protease‐activated receptor 1‐ and protease‐activated receptor 4‐dependent mechanism) Thrombin modifies growth, proliferation and apoptosis of human colon organoids: a protease‐activated receptor 1‐ and protease‐activated receptor 4‐dependent mechanism
    Article Snippet: Tissue obtained was opened longitudinally and washed with Dulbecco's PBS (DPBS; Sigma‐Aldrich #D8537, Saint‐Quentin‐Fallavier, France). .. Then, the mucosa was removed and incubated in an antibiotic solution (gentamicin Sigma‐Aldrich #G1397, Saint‐Quentin‐Fallavier, France, 1000×; normocin InvivoGen #ANT‐NR‐1, Toulouse, France, 500×; and fungizone Gibco #15290‐018, Illkirch, France, 100×) for 5 min at room temperature (RT), four times.

    Article Title: Downregulation of endometrial mesenchymal marker SUSD2 causes cell senescence and cell death in endometrial carcinoma cells
    Article Snippet: The supernatant was discarded and 1 ml of -20°C ice-cold ethanol (#20821.330, VWR Chemicals, USA): PBS (#D8537, Sigma) mixture (3:1) was added to the pellet during vortexing. .. The mixture was kept at -20°C overnight, the next day washed with PBS again, 250 μl PI mix containing 50 μg/ml PI (#P4864, Sigma) and 100 μg/ml RNase A (#R4642, Sigma) were added, incubated for 30 min at 37°C, and subjected to flow cytometry for cell cycle and death analysis.

    Article Title: Assessment of the efficacy of different procedures that remove and disassemble alpha-synuclein, tau and A-beta fibrils from laboratory material and surfaces
    Article Snippet: Aggregation of protein into amyloid fibrils α-Syn was incubated in buffer A to obtain the fibrillar polymorph “fibrils” (50 mM Tris–HCl, pH 7.5, 150 mM KCl), in buffer B for “ribbons” (5 mM Tris–HCl, pH 7.5), in buffer C for “fibril-65” (20 mM MES pH6,5, 150 mM NaCl) and in buffer D for “fibril-91” (20 mM KPO4 pH9.1), at 37 °C under continuous shaking in an Eppendorf Thermomixer set at 600 r.p.m for 4–7 days , . .. Aß fibrillation was achieved at 500 µM in PBS (Sigma, #D8537) at 37 °C under continuous shaking in an Eppendorf Thermomixer set at 600 r.p.m for 4 days.

    Article Title: Toll-Like Receptor Ligands and Interferon-γ Synergize for Induction of Antitumor M1 Macrophages
    Article Snippet: .. Macrophages were harvested by incubation for 30 min at 4°C with cold PBS without CaCl2 and MgCl2 (#D8537, Sigma-Aldrich). .. Macrophages were then flushed off the plate, collected and kept frozen in aliquots at −150°C in FBS with 10% dimethyl sulfoxide (#0231-500 ml, VWR) for future experiments.

    Article Title: Thrombin modifies growth, proliferation and apoptosis of human colon organoids: a protease‐activated receptor 1‐ and protease‐activated receptor 4‐dependent mechanism) Thrombin modifies growth, proliferation and apoptosis of human colon organoids: a protease‐activated receptor 1‐ and protease‐activated receptor 4‐dependent mechanism
    Article Snippet: Organoid cultures Tissue obtained was opened longitudinally and washed with Dulbecco's PBS (DPBS; Sigma‐Aldrich #D8537, Saint‐Quentin‐Fallavier, France). .. Then, the mucosa was removed and incubated in an antibiotic solution (gentamicin Sigma‐Aldrich #G1397, Saint‐Quentin‐Fallavier, France, 1000×; normocin InvivoGen #ANT‐NR‐1, Toulouse, France, 500×; and fungizone Gibco #15290‐018, Illkirch, France, 100×) for 5 min at room temperature (RT), four times.

    Cell Culture:

    Article Title: Crossing the blood-brain-barrier with nanoligand drug carriers self-assembled from a phage display peptide
    Article Snippet: Dulbecco/Vogt Modified Eagle's Minimal Essential Medium (DMEM) (#D0819), basic fibroblast growth factor (#F0291), gelatin solution (#G1393), hydrocortisone (#H-0888), insulin (#I-1882) and phosphate buffered saline (PBS) (#D8537) were purchased from Sigma-Aldrich Co. LLC. (Seelze, Germany). .. HEPES (#S11-001) was from PAA Cell Culture Company (Pasching, Austria).

    Article Title: Toll-Like Receptor Ligands and Interferon-γ Synergize for Induction of Antitumor M1 Macrophages
    Article Snippet: The bone marrow cells were cultured for 5 days, after which non-adherent, cells were washed off using phosphate buffered saline (PBS, # D8662, Sigma-Aldrich) and the adherent macrophages were cultured for 1 more day. .. Macrophages were harvested by incubation for 30 min at 4°C with cold PBS without CaCl2 and MgCl2 (#D8537, Sigma-Aldrich).

    Sampling:

    Article Title: Radiosensitivity in breast cancer assessed by the Comet and micronucleus assays
    Article Snippet: Paragraph title: Blood sampling and isolation of cells ... Peripheral blood mononuclear cells were washed twice with Ca2+ - and Mg2+ -free physiological phosphate-buffered saline (PBS; Sigma D-8537) and finally resuspended in the freezing medium containing RPMI 1640 (Sigma R-8758), 10% foetal calf serum and 10% dimethyl sulphoxide, and stored frozen in a liquid nitrogen until analysis.

    Modification:

    Article Title: Crossing the blood-brain-barrier with nanoligand drug carriers self-assembled from a phage display peptide
    Article Snippet: .. Dulbecco/Vogt Modified Eagle's Minimal Essential Medium (DMEM) (#D0819), basic fibroblast growth factor (#F0291), gelatin solution (#G1393), hydrocortisone (#H-0888), insulin (#I-1882) and phosphate buffered saline (PBS) (#D8537) were purchased from Sigma-Aldrich Co. LLC. (Seelze, Germany). .. Foetal bovine serum (FBS) (#16000044) and penicillin/streptomycin (#15140122) were obtained from Gibco-BRL, Carlsbad Life Technologies (CA, USA).

    Chloramphenicol Acetyltransferase Assay:

    Article Title: Spheroid-Formation (Colonosphere) Assay for in Vitro Assessment and Expansion of Stem Cells in Colon Cancer
    Article Snippet: .. Phosphate buffered saline (PBS) (CatD8537), Poly-L-lysine (Cat#P4707), RPMI-1640 (Roswell Park Memorial Institute) (Cat#R0883), FBS (Fetal Bovine Serum) (Cat#F7524) all purchased from Sigma Aldrich. .. Preparation of Poly-L-Lysine-Coated Coverslips in Sterile 6-well Cell Culture Plate Immerse your sterile uncoated coverslips of 22 × 26 mm thickness in 70 % ethanol for 30 min. Wash coverslips twice in 1 ml sterile phosphate buffered saline (without calcium/magnesium chloride) (PBS) for 5 min each.

    Recombinant:

    Article Title: Crossing the blood-brain-barrier with nanoligand drug carriers self-assembled from a phage display peptide
    Article Snippet: Dulbecco/Vogt Modified Eagle's Minimal Essential Medium (DMEM) (#D0819), basic fibroblast growth factor (#F0291), gelatin solution (#G1393), hydrocortisone (#H-0888), insulin (#I-1882) and phosphate buffered saline (PBS) (#D8537) were purchased from Sigma-Aldrich Co. LLC. (Seelze, Germany). .. Recombinant human EGF (AF-100-15) was from PeproTech EC Ltd. (Hamburg, Germany).

    Immunofluorescence:

    Article Title: Development of a low-pressure microtargeting biolistic device for transfection of retinal explants
    Article Snippet: Paragraph title: Immunofluorescence assays ... At the conclusion of experiments, explants were rinsed once in Dulbecco’s PBS (#D8537; Sigma-Aldrich Co. LLC) and fixed with 4% paraformaldehyde in PBS containing 4% sucrose for 1 h at room temperature.

    Derivative Assay:

    Article Title: Influence of environmental conditions on the attenuation of ricin toxin on surfaces
    Article Snippet: In addition, a crude preparation of the toxin was extracted from whole castor beans (Vector Laboratories, Inc.), and was prepared in the laboratory using methods derived from the scientific literature [ ]. .. Whole castor beans were de-husked and homogenized into a slurry, precipitated from the solution, dialyzed, and rinsed with sterile PBS (Cat #D8537 Sigma-Aldrich, St. Louis, MO).

    Article Title: Toll-Like Receptor Ligands and Interferon-γ Synergize for Induction of Antitumor M1 Macrophages
    Article Snippet: Paragraph title: Generation of Bone Marrow Derived Macrophages ... Macrophages were harvested by incubation for 30 min at 4°C with cold PBS without CaCl2 and MgCl2 (#D8537, Sigma-Aldrich).

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  • 79
    Millipore cytokine homogenate buffer
    Leptin and <t>cytokine</t> levels (IL-6 and TNF- α ) after 4 and 6 weeks on the diets. Consistent with the weight gain and epididymal fat pad weights, leptin levels were significantly higher after 4 and 6 weeks in the HAGE-HF group compared to the other groups. Also consistent with the liver histology and presence of inflammation after 6 weeks on the diet, IL-6 and TNF- α levels were significantly higher in the HAGE-HF group after 6 weeks on the diet.
    Cytokine Homogenate Buffer, supplied by Millipore, used in various techniques. Bioz Stars score: 79/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cytokine homogenate buffer/product/Millipore
    Average 79 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cytokine homogenate buffer - by Bioz Stars, 2020-02
    79/100 stars
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    83
    Millipore annexin buffer
    Apoptosis, loss of ΔΨ m, and ROS production are caspase dependent. HeLa cells were treated with actinomycin D or UV (as indicated) in the presence or absence of 50 μM of zVAD-fmk, harvested, and stained with <t>annexin</t> V–FITC and PI to assess cell death, TMRE to measure ΔΨm, and 2-HE to measure ROS production, and then analyzed by flow cytometry.
    Annexin Buffer, supplied by Millipore, used in various techniques. Bioz Stars score: 83/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/annexin buffer/product/Millipore
    Average 83 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    annexin buffer - by Bioz Stars, 2020-02
    83/100 stars
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    78
    Millipore oocyte ringers hco3
    Apoptosis, loss of ΔΨ m, and ROS production are caspase dependent. HeLa cells were treated with actinomycin D or UV (as indicated) in the presence or absence of 50 μM of zVAD-fmk, harvested, and stained with <t>annexin</t> V–FITC and PI to assess cell death, TMRE to measure ΔΨm, and 2-HE to measure ROS production, and then analyzed by flow cytometry.
    Oocyte Ringers Hco3, supplied by Millipore, used in various techniques. Bioz Stars score: 78/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/oocyte ringers hco3/product/Millipore
    Average 78 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    oocyte ringers hco3 - by Bioz Stars, 2020-02
    78/100 stars
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    99
    Millipore pbm
    Apoptosis, loss of ΔΨ m, and ROS production are caspase dependent. HeLa cells were treated with actinomycin D or UV (as indicated) in the presence or absence of 50 μM of zVAD-fmk, harvested, and stained with <t>annexin</t> V–FITC and PI to assess cell death, TMRE to measure ΔΨm, and 2-HE to measure ROS production, and then analyzed by flow cytometry.
    Pbm, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pbm/product/Millipore
    Average 99 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    pbm - by Bioz Stars, 2020-02
    99/100 stars
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    Image Search Results


    Leptin and cytokine levels (IL-6 and TNF- α ) after 4 and 6 weeks on the diets. Consistent with the weight gain and epididymal fat pad weights, leptin levels were significantly higher after 4 and 6 weeks in the HAGE-HF group compared to the other groups. Also consistent with the liver histology and presence of inflammation after 6 weeks on the diet, IL-6 and TNF- α levels were significantly higher in the HAGE-HF group after 6 weeks on the diet.

    Journal: BioMed Research International

    Article Title: Advanced Glycation End Products Induce Obesity and Hepatosteatosis in CD-1 Wild-Type Mice

    doi: 10.1155/2016/7867852

    Figure Lengend Snippet: Leptin and cytokine levels (IL-6 and TNF- α ) after 4 and 6 weeks on the diets. Consistent with the weight gain and epididymal fat pad weights, leptin levels were significantly higher after 4 and 6 weeks in the HAGE-HF group compared to the other groups. Also consistent with the liver histology and presence of inflammation after 6 weeks on the diet, IL-6 and TNF- α levels were significantly higher in the HAGE-HF group after 6 weeks on the diet.

    Article Snippet: The remaining liver was washed with cold HBSS and suspended in cytokine homogenate buffer (150 mM NaCl, 15 mM Tris, 1 mM CaCl2 ·2H2 O, and 1 mM MgCl2 ·6H2 O, adjusted to pH 7.4) plus 100x protease inhibitor cocktail 1 (Calbiochem, La Jolla, California) to a total volume of 10 mL and homogenized on ice using a Polytron® Homogenizer (Kinematica Inc., Bohemia, NY).

    Techniques:

    Apoptosis, loss of ΔΨ m, and ROS production are caspase dependent. HeLa cells were treated with actinomycin D or UV (as indicated) in the presence or absence of 50 μM of zVAD-fmk, harvested, and stained with annexin V–FITC and PI to assess cell death, TMRE to measure ΔΨm, and 2-HE to measure ROS production, and then analyzed by flow cytometry.

    Journal: The Journal of Cell Biology

    Article Title: Caspase-mediated loss of mitochondrial function and generation of reactive oxygen species during apoptosis

    doi: 10.1083/jcb.200208089

    Figure Lengend Snippet: Apoptosis, loss of ΔΨ m, and ROS production are caspase dependent. HeLa cells were treated with actinomycin D or UV (as indicated) in the presence or absence of 50 μM of zVAD-fmk, harvested, and stained with annexin V–FITC and PI to assess cell death, TMRE to measure ΔΨm, and 2-HE to measure ROS production, and then analyzed by flow cytometry.

    Article Snippet: The pellet was resuspended in 30 μl of annexin buffer (Hepes 10 mM, NaCl 150 mM, KCl 5 mM, MgCl2 1 mM, CaCl2 1.8 mM) and then divided into three groups: one third each for ΔΨm measurement (as described above), ROS measurement using 2 μM of 2-HE in MIB buffer, and cell death measurement using annexin V–FITC (Calbiochem).

    Techniques: Staining, Flow Cytometry, Cytometry